1. Adipokine vaspin maintains angiogenesis and neurological function during cerebral ischemia-reperfusion via suppressing endoplasmic reticulum stress.
- Author
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Yan W, He X, Wang G, Hu G, and Cui B
- Subjects
- Animals, Humans, Male, Mice, Apoptosis drug effects, Disease Models, Animal, Endothelial Cells metabolism, Endothelial Cells drug effects, Infarction, Middle Cerebral Artery metabolism, Infarction, Middle Cerebral Artery prevention & control, Neovascularization, Physiologic drug effects, Adipokines administration & dosage, Adipokines metabolism, Adipokines pharmacology, Angiogenesis drug effects, Angiogenesis metabolism, Brain Ischemia drug therapy, Brain Ischemia metabolism, Endoplasmic Reticulum Stress drug effects, Reperfusion Injury metabolism, Reperfusion Injury prevention & control, Serpins metabolism, Serpins pharmacology
- Abstract
Introduction: Visceral adipose tissue-derived serine protease inhibitor (vaspin) is an adipokine. It has been reported that decreased serum vaspin levels are significantly associated with stroke severity and prognosis., Objective: This article aims to explore the theoretical feasibility of vaspin supplementation for cerebral ischemia-reperfusion (I/R) injury., Methods: The I/R mouse models were constructed by the middle cerebral artery occlusion (MCAO) method, and the effects of vaspin on cerebral infarction, neurological function, angiogenesis and endoplasmic reticulum (ER) stress were explored. To verify the mediation of ER stress in the regulation of vaspin, human brain microvascular endothelial cells (HBMECs) were subjected to ER stress agonist tunicamycin in vitro. The impacts of vaspin and tunicamycin on oxygen glucose deprivation/ recovery (OGD/R)-induced cell viability, apoptosis, and angiogenesis were examined., Results: Vaspin inhibited blood-brain barrier breakdown and infarction occurred in the brain tissue of the I/R mice. Vaspin also enhanced cerebral neovascularization and reduced the apoptosis. Additional tunicamycin increased the apoptosis of HBMECs and inhibited angiogenesis, reversing the protective effect of vaspin on cells., Conclusion: Together, this study reveals that vaspin supplementation reduces cerebral infarction and works against neurological dysfunction. It maintains the survival and angiogenesis capacity of HBMECs by inhibiting ER stress.
- Published
- 2024
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