1. A Microfluidic Device for Rapid Screening of E. coli O157:H7 Based on IFAST and ATP Bioluminescence Assay for Water Analysis.
- Author
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Ngamsom B, Truyts A, Fourie L, Kumar S, Tarn MD, Iles A, Moodley K, Land KJ, and Pamme N
- Subjects
- Escherichia coli O157 metabolism, Firefly Luciferin chemistry, Firefly Luciferin metabolism, Lab-On-A-Chip Devices, Light, Luciferases metabolism, Luminescent Measurements instrumentation, Surface Tension, Adenosine Triphosphate metabolism, Escherichia coli O157 isolation & purification, Luminescent Measurements methods, Wastewater microbiology
- Abstract
We present a simple microfluidic system for rapid screening of Escherichia coli (E. coli) O157:H7 employing the specificity of immunomagnetic separation (IMS) via immiscible filtration assisted by surface tension (IFAST), and the sensitivity of the subsequent adenosine triphosphate (ATP) assay by the bioluminescence luciferin/luciferase reaction. The developed device was capable of detecting E. coli O157:H7 from just 6 colony forming units (CFU) in 1 mL spiked buffer within 20 min. When tested with wastewater discharged effluent samples, without pre-concentration, the device demonstrated the ability to detect 10
4 CFU per mL seeded; suggesting great potential for point-of-need microbiological water quality monitoring., (© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)- Published
- 2017
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