A Microfluidic Device for Rapid Screening of E. coli O157:H7 Based on IFAST and ATP Bioluminescence Assay for Water Analysis.

Authors :: Ngamsom B
Truyts A
Fourie L
Kumar S
Tarn MD
Iles A
Moodley K
Land KJ
Pamme N
Source :: Chemistry (Weinheim an der Bergstrasse, Germany) [Chemistry] 2017 Sep 18; Vol. 23 (52), pp. 12754-12757. Date of Electronic Publication: 2017 Aug 18.
Publication Year :: 2017
Abstract: We present a simple microfluidic system for rapid screening of Escherichia coli (E. coli) O157:H7 employing the specificity of immunomagnetic separation (IMS) via immiscible filtration assisted by surface tension (IFAST), and the sensitivity of the subsequent adenosine triphosphate (ATP) assay by the bioluminescence luciferin/luciferase reaction. The developed device was capable of detecting E. coli O157:H7 from just 6 colony forming units (CFU) in 1 mL spiked buffer within 20 min. When tested with wastewater discharged effluent samples, without pre-concentration, the device demonstrated the ability to detect 10 <superscript>4</superscript> CFU per mL seeded; suggesting great potential for point-of-need microbiological water quality monitoring.<br /> (© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Subjects :: Escherichia coli O157 metabolism
Firefly Luciferin chemistry
Firefly Luciferin metabolism
Lab-On-A-Chip Devices
Light
Luciferases metabolism
Luminescent Measurements instrumentation
Surface Tension
Adenosine Triphosphate metabolism
Escherichia coli O157 isolation & purification
Luminescent Measurements methods
Wastewater microbiology

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