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Made available in DSpace on 2018-11-26T17:42:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-11-01 Locomotion issues in broiler production may decrease performance (carcass yield and traits) and lead to high financial losses. This study evaluates the addition of glucosaminoglycans in broiler diets to minimize the lack of proper bone development and joint weakening. The experiment was conducted using 2,160 broilers randomly distributed in a factorial pattern (3 x 3) using 3 levels of glucosamine sulfate (0, 0.12, and 0.24%) and 3 levels of chondroitin sulfate addition (0, 0.08, and 0.16%). Eight repetitions were used for each treatment, distributed in 72 pens with 30 broilers each. There was a quadratic effect on feed conversion for broilers from 1 to 42 d old (P = 0.0123) for the addition of chondroitin, and better feed conversion was obtained by adding 0.08% of chondroitin. The relative tibia weight, the width of the proximal epiphysis and diaphysis presented a linear increased effect in broilers at 42 d old. An interaction was found between the amount of chondroitin x glucosamine and the number of chondrocytes in the proximal cartilage of the tibia (P = 0.0072). There was a quadratic effect of glucosamine levels (P = 0.0107) in the birds that had received the 0.16% addition of chondroitin, and the presence of 0.18% glucosamine increased the number chondrocytes in the cartilage of broilers. These results provide the first evidence that broilers may benefit from increased dietary chondroitin sulfate. These results indicate that the addition of glucosamine and chondroitin sulfates in broiler feed rations might alleviate leg conditions and decrease financial losses in the broiler industry. Brazil Univ, Descalvado, SP, Brazil Sao Paulo State Univ, Dept Morphol & Anim Physiol, Jaboticabal, SP, Brazil Fed Univ Grande Dourados, Coll Agr Sci, Dourados, MS, Brazil Sao Paulo State Univ, Dept Bioengn, Ribeirao Preto, SP, Brazil Sao Paulo State Univ, Dept Morphol & Anim Physiol, Jaboticabal, SP, Brazil Sao Paulo State Univ, Dept Bioengn, Ribeirao Preto, SP, Brazil

Made available in DSpace on 2019-10-04T12:39:12Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-05-04 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) 1. This study analysed whether in ovo injection of ascorbic acid before incubation and at high incubation temperature influenced blood characteristics and performance in broilers reared in different temperature conditions.2. A total of 3,000 fertile eggs from broiler breeders (Cobb (R)) were randomly divided into three incubation treatments: no ascorbic acid injection and egg incubation at 37.5 degrees C (control); no ascorbic acid injection and egg incubation at 39 degrees C; in ovo ascorbic acid injection prior to incubation (6 mu g AA/100 mu l water) and egg incubation at 39 degrees C.3. Male chicks hatched from the three incubation treatments were submitted to three distinct rearing temperatures (control, cold and hot) from the third week of age onwards (540 chicks were divided into 6 treatments with 5 replicates per treatment).4. Measurements at 42d showed that, after egg incubation at 39 degrees C, the haematocrit, haemoglobin values, ionised calcium and glucose concentrations were increased and base excess values were reduced. However, in ovo injection of ascorbic acid normalised all these parameters.5. Partial CO2 and O-2 pressure were higher with increased rearing temperature. Blood pH was lower when eggs were incubated at 39 degrees C and injected with ascorbic acid. In ovo injection of ascorbic acid induced leucocytosis due to lymphocytosis and heterophilia, restored basophils rate and led to monocytopoenia. Leucocytosis was triggered by hot rearing temperature due to lymphocytosis, eosinophilia and heterophilia.6. The results obtained in this study showed that in ovo injection of ascorbic acid before incubation may serve as a long-term stimulator and modulator of the broiler immune system, and that high incubation temperatures induce adaptations in the electrolytic balance, minimising or avoiding the occurrence of respiratory alkalosis under hot rearing temperature. Brazil Univ, Anim Prod Master Program, Descalvado, SP, Brazil Sao Paulo State Univ, Fac Agron & Vet Sci, Dept Anim Morphol & Physiol, Jaboticabal, SP, Brazil Sao Paulo State Univ, Fac Agron & Vet Sci, Dept Anim Morphol & Physiol, Jaboticabal, SP, Brazil FAPESP: 2010/01923-7 FAPESP: 2010/15280-0

Made available in DSpace on 2018-11-26T17:55:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-09-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) For the production and commercialization of ornamental fish species, it is indispensable to collect biometric data that facilitate the selection of animals for trade and genetic improvement of the stock. However, during the handling process, fish receive more stress if proper anesthetics are not used. Thus, application of appropriate anesthetics is an important tool for minimizing stress in animals. The objective of this study was to determine the effective concentrations of benzocaine, eugenol, and menthol for achieving anesthesia in Freshwater Angelfish Pterophyllum scalare and to develop induction and recovery response curves for different concentrations of these anesthetics. In total, 75 fish were exposed to five concentrations of the three anesthetics in a completely randomized design: benzocaine at 60, 85, 110, 135, and 160mg/L; eugenol at 40, 80, 120, 160, and 200mg/L; and menthol at 50, 75, 150, 200, and 250mg/L. Each concentration (5fish/concentration) consisted of five replicates, with each replicate represented by a single fish. The results indicated that the tested substances met the criteria of anesthetic efficiency. The effective concentrations of benzocaine, eugenol, and menthol for the anesthesia of Freshwater Angelfish were identified as 89.25, 90.6, and 92.1mg/L, respectively. Sao Paulo State Univ, Aquaculture Ctr, Via Acesso Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, Brazil Brazil Univ, Campus Fernandopolis,Estr Projetada F-1 S-N, BR-15600000 Fernandopolis, SP, Brazil Sao Paulo State Univ, Aquaculture Ctr, Via Acesso Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, Brazil FAPESP: 2016/11940-2 FAPESP: 2013/25761-4

Made available in DSpace on 2018-11-26T17:16:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-10-01. Added 1 bitstream(s) on 2021-07-15T15:01:35Z : No. of bitstreams: 1 S1516-635X2016000600041.pdf: 244221 bytes, checksum: 7af79ecb06a75851f7e834f2a94cff5b (MD5) Adequate environmental temperature control is essential for incubation efficiency. Layer breeder eggs (n=360) were weighed and distributed in a completely randomized experimental design with two treatments, consisting of two incubation temperatures (T1=37.5 degrees C, control; and T2=39.0 degrees C, hot), with two incubators per temperature, and 90 eggs per incubator, totaling 360 eggs. Hatchability, embryo mortality, and chick cloacal and body surface temperatures were not affected by incubation temperature. Eggs incubated at the hot temperature presented greater egg mass loss and higher eggshell conductance than those incubated at the control temperature. Layer chicks derived from eggs incubated at control temperature showed greater absolute weight, yolk-free egg mass, and heavier hearts than those from eggs submitted to heat stress during incubation. The control group presented lower base excess and ionized calcium blood levels. Incubating eggs at temperatures higher than those recommended compromises body and heart development of layer chicks and negatively affects blood ionized calcium availability, and therefore, bone mineralization during embryo development. Efficient temperature control during the incubation of fertile eggs is essential to obtain good quality layer chicks. Brazil Univ, BR-13690970 Descalvado, SP, Brazil Sao Paulo State Univ, Dept Morphol & Anim Physiol, Anim Sci, BR-14884900 Jaboticabal, SP, Brazil Fed Univ Grande Dourados, Postdoctoral Program Agribusiness, Coll Agr Sci, BR-79804970 Dourados, MS, Brazil Sao Paulo State Univ, Dept Morphol & Anim Physiol, BR-14884900 Jaboticabal, SP, Brazil Fed Univ Grande Dourados, Coll Agr Sci, BR-79804970 Dourados, MS, Brazil Sao Paulo State Univ, Dept Morphol & Anim Physiol, Anim Sci, BR-14884900 Jaboticabal, SP, Brazil Sao Paulo State Univ, Dept Morphol & Anim Physiol, BR-14884900 Jaboticabal, SP, Brazil