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To describe a method of measuring the molar cusp deformation using micro-computed tomography (micro-CT), the propagation of enamel cracks using transillumination, and the effects of hygroscopic expansion after incremental and bulk-filling resin composite restorations.Twenty human molars received standardized Class II mesio-occlusal-distal cavity preparations. They were restored with either a bulk-fill resin composite, X-tra fil (XTRA), or a conventional resin composite, Filtek Z100 (Z100). The resin composites were tested for post-gel shrinkage using a strain gauge method. Cusp deformation (CD) was evaluated using the images obtained using a micro-CT protocol and using a strain-gauge method. Enamel cracks were detected using transillumination.The post-gel shrinkage of Z100 was higher than XTRA (P 0.001). The amount of cusp deformation produced using Z100 was higher compared to XTRA, irrespective of the measurement method used (P 0.001). The thinner lingual cusp always had a higher CD than the buccal cusp, irrespective of the measurement method (P 0.001). A positive correlation (r = 0.78) was found between cusp deformation measured by micro-CT or by the strain-gauge method. After hygroscopic expansion of the resin composite, the cusp displacement recovered around 85% (P 0.001). After restoration, Z100 produced more cracks than XTRA (P = 0.012).Micro-CT was an effective method for evaluating the cusp deformation. Transillumination was effective for detecting enamel cracks. There were fewer negative effects of polymerization shrinkage in bulk-fill resin restorations using XTRA than for the conventional incremental filling technique using conventional composite resin Z100.Shrinkage and cusp deformation are directly related to the formation of enamel cracks. Cusp deformation and crack propagation may increase the risk of tooth fracture.

CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico FAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais This in vitro study aimed to assess the effects of different pretreatments used to adapt glass-fiber posts cemented to root canals with different resin cements, regarding porosity percentage and bond strength. Twelve bovine incisor roots were prepared with Largo drills. After post space preparation, the specimens were randomized into two types of pretreatment groups (n = 3): water-wet bonding and ethanol-wet bonding. After the post were cemented, the roots were stored in 100% humidity at room temperature for 7 days. The samples were scanned by microcomputed tomography (micro-CT). Images were reconstructed by NRecon software, and CTAn software was used to analyze the porosity percentage (%) at the luting interface. Evaluation of the push-out bond strength was performed by serially cutting the roots, and submitting the slices to testing. Additionally, the resin cement post-gel shrinkage values (%) were measured using the strain-gauge method (n = 10). Data were analyzed by two-way ANOVA, Tukey's test and Student's t test (a = 0.05). The roots prepared with ethanol-wet bonding using RelyX™ U200 had significantly lower porosity in the coronal and apical thirds (p < 0.05). The group prepared with ethanol-wet bonding using RelyX™ ARC presented better bond strength results in the coronal and apical thirds (p < 0.05). RelyX™ ARC (0.97%) produced a higher post-gel shrinkage value than RelyX™ U200 (0.77%). Canals pretreated with ethanol-wet bonding presented better outcomes in regard to porosity percentage and push-out bond strength

The aim of this study was to evaluate the effect of magnification during post space preparation on root cleanness and on fiber post bond strength. Methods: Thirty human central upper incisors with similar root canal in size and shape were selected, decoronated to 15 mm and endodontically filled. The teeth were assigned into 3 groups (n=10), according to the method of magnification during post space preparation inspection: Control, using naked eye; loupe, using a dental surgical 3x magnifying glass; surgical microscope, using a 6x surgical microscope. The roots were scanned by using micro-CT before and after post space preparation for residue remnants evaluation. Fiber posts were cemented using self-adhesive resin cement (Rely X U200, 3M-ESPE). Two 1-mm-thick slices from the cervical, medium and apical thirds were submitted to a push-out test (PBS). Failures modes were classified. PBS data were analyzed by using two-way ANOVA with repeated measurement and the Tukey test. The significance level was set at 5%. The method of visualization had no effect on PBS (p=0.556). The cervical region had higher values than apical region irrespective of the inspection method (p=0.012). Adhesive failure between the resin cement and dentin was the prevalent failure mode for all groups. Micro-CT analysis showed no difference on root cleanness into the root canal after post space preparation. The use of magnification devices as loupe and microscope while performing post space do not improve the PBS and did not affect sealer remain of decoronated anterior teeth. Resumo Avaliar o efeito do método de visualização durante o preparo do espaço do pino sobre o remanescente de material obturador endodôntico e sobre a resistência adesiva do pino de fibra de vidro. Trinta incisivos centrais superiores humanos com canal radicular circular foram selecionados, foram desobstruídos a 15 mm e obturados. Os dentes foram divididos em 3 grupos (n=10), de acordo com o método de ampliação utilizado para inspeção do preparo: Controle, usando olho nu; lupa, usando uma lupa cirúrgica 3x cirúrgica; microscópio cirúrgico, usando um microscópio cirúrgico 6x. As raízes foram digitalizadas usando micro-CT antes e após a preparação do espaço para avaliação de resíduos remanescentes. Os pinos de fibra foram cimentados com cimento resinoso autoadesivo (RelyX U200, 3M-ESPE). Duas fatias de 1 mm de espessura dos terços cervical, médio e apical foram submetidas ao teste push-out (PBS). Os padrões de falhas foram classificados. Os dados de PBS foram analisados usando análise de variância em dois fatores com medição repetida e o teste de Tukey. O nível de significância foi estabelecido em 5%. Resultados: O método de visualização não teve efeito no PBS (p=0,556). A região cervical apresentou valores maiores que a região apical, independentemente do método de inspeção (p=0,012). A falha adesiva entre o cimento resinoso e a dentina foi o modo de falha prevalente para todos os grupos. A análise de micro-CT não mostrou diferença na limpeza da raiz no canal radicular após a preparação do espaço do pino. Conclusão: O uso de dispositivos de ampliação como lupas e microscópios durante a realização de preparo não melhora o PBS e não afetou a permanência do material obturador remanescente de dentes anteriores.

The aim of this study was to evaluate the biomechanical properties expressed by shrinkage stress, cuspal strain, fracture strength and failure mode in molars with large class II mesio-occlusal-distal restorations. Sixty-four human caries-free third molars were selected and distributed randomly into four groups: Z100 restorative material (Z100), Tetric N-Ceram Bulk-Fill (TNC), Filtek Bulk-Fill (FBF) and Aura Ultra Universal (ABF). The bulk-fill materials were inserted in one singular bulk increment and the conventional composite resin in three ones. Polymerisation shrinkage stress was evaluated by optical Fibre Bragg Gratings (FBG) sensors (n = 6). The cuspal deformation was measured using an extensometer during three moments: restorative procedure, axial compressive loading and at fracture (n = 10). The fracture strength was evaluated on a universal machine. The failure mode was analysed by Scanning Electron Microscopy (SEM). Data were analysed using one-way ANOVA tests with Tukey's posthoc test (α = 5%). Data of the failure mode were submitted to a likelihood ratio chi-square test. Z100 presented the highest mean value for the shrinkage stress (p 0.05) by FBG evaluation, whereas bulk-fill resin groups presented low polymerisation stress mean value, especially the TNC (p 0.05). The cuspal deformation test showed that Z100 presented a significant difference mean value compared to the other groups (p 0.01) during the restoration and compressive axial force; however, load until the fracture presented a difference only between TNC and FBF (p 0.05). The fracture strength of TNC was statistically different from Z100 (p 0.01). The failure mode was not statistically different in all the groups (p 0.05). Bulk-fill composites promoted less polymerisation shrinkage stress than conventional microhybrid composite during and after the light curing process in class II posterior resin composite restorations.

Background/aim Custom-fitted mouthguards are devices used to prevent dental injuries. The aim of this study was to verify the influence of the antagonist contact on the stresses and strains of the anterior teeth, shock absorption and displacement of EVA custom-fitted mouthguards during a horizontal impact. Materials and methods Finite element models of human maxillary central incisors with and without a mouthguard for different occlusion conditions (with and without antagonist contact) were created based on tomography. A nonlinear dynamic impact analysis using the single-step Houbolt method was performed in which a rigid object hit the model at 1 m s-1 . Strain and stress were evaluated by means of Von Mises and Critical modified Von Mises criterion and shock absorption during impact were calculated as well as the mouthguard displacement. Results The model without mouthguard and without antagonist contact showed the highest stress and strain values at the enamel and dentin in the tooth crown on impact compared to the model without mouthguard and with antagonist contact. Mouthguard presence reduced the stress and strain values regardless of the occlusion condition. The mouthguard displacement decreased with the mandibular antagonist contact. Conclusions Mouthguards are efficient at decreasing the stress and strain values on the tooth in front of an impact reaching more than 90% of shock absorption. A mouthguard with balanced occlusion and maximum number of contacts with mandibular anterior teeth should be considered because it reduces mouthguard displacement.

SUMMARY Objectives To evaluate the effect of the restorative protocol on cuspal strain, fracture resistance, residual stress, and mechanical properties of restorative materials in endodontically treated molars. Methods Forty-five molars received mesio-occlusal-distal (MOD) Class II preparations and endodontic treatment followed by direct restorations using three restorative protocols: composite resin (CR) only (Filtek Supreme, 3M-ESPE), resin modified glass ionomer cement in combination with CR (Vitremer, 3M-ESPE in pulp chamber and Filtek Supreme in MOD cavity), conventional glass ionomer cement in combination with composite resin (CGI-CR) (Ketac Fil, 3M-ESPE in pulp chamber and Filtek Supreme in MOD cavity). Cuspal strain was measured using strain gauges, and fracture resistance was tested with an occlusal load. Elastic modulus (EM) and Vickers hardness (VH) of the restorative materials were determined at different depths using dynamic microhardness indentation. Curing shrinkage was measured using the strain gauge technique. The restorative protocols were also simulated in finite element analysis (FEA). The shrinkage strain, cuspal strain, EM, VH, and fracture resistance data were statistically analyzed using split-plot analysis of variance and Tukey test (p=0.05). Residual shrinkage stresses were expressed in modified von Mises equivalent stresses. Results Shrinkage strain values (in volume %) were Ketac Fil (0.08±0.01) < Vitremer (0.18±0.01) < Filtek Supreme (0.54±0.03). Cuspal strain was higher and fracture resistance was lower when using CR only compared with the techniques that used glass ionomer. The EM and VH of the materials in the pulp chamber were significantly lower for glass ionomer. The FEA showed that using CR only resulted in higher residual stresses in enamel and root dentin close to the pulp chamber than the combinations with glass ionomers (RMGI-CR and CGI-CR). Conclusions The choice of restorative protocol significantly affected the biomechanical behavior of endodontically treated molars. Using glass ionomer to fill the pulp chamber is recommended when endodontically treated molars receive direct composite restorations because it reduces cuspal strain and increases fracture resistance.

Objective This study aimed to evaluate the effect of the cavity preparation and ceramic type on the stress distribution, tooth strain, fracture resistance and fracture mode of human molar teeth restored with onlays. Material and Methods Forty-eight molars were divided into four groups (n=12) with assorted combinations of two study factors: BL- conventional onlay preparation with boxes made from leucite ceramic (IPS-Empress CAD, Ivoclar Vivadent); NBL- conservative onlay preparation without boxes made from leucite ceramic; BD- conventional onlay preparation with boxes made from lithium disilicate glass ceramic (IPS e.max CAD, Ivoclar Vivadent); NBL- conservative onlay preparation with boxes made from lithium disilicate glass ceramic cuspal deformation (µS) was measured at 100 N and at maximum fracture load using strain gauge. Fracture resistance (N) was measured using a compression test, and the fracture mode was recorded. Finite element analysis was used to evaluate the stress distribution by modified von Mises stress criteria. The tooth strain and fracture resistance data were analyzed using the Tukey test and two-way ANOVA, and the fracture mode was analyzed by the chi-square test (α=0.05). Results The leucite ceramic resulted in higher tooth deformation at 100 N and lower tooth deformation at the maximum fracture load than the lithium disilicate ceramic (P

Posterior composite restorations may be negatively affected by acidic and colored drinks. Little information is available about the effect of acidic drinks on bulk-fill composite resins.The purpose of this in vitro study was to investigate the effect of acidic drinks on the different properties of conventional and bulk-fill composite resins.A conventional composite resin and 2 representative bulk-fill composite resins were used to prepare disk-shaped specimens. The degree of conversion (DC) was monitored by spectroscopy (n=5). The specimens were divided into 5 groups: control, artificial saliva, acai juice, red wine, and Coca-Cola and were maintained for 30 days of challenge (3 periods of 15 min/d). Surface topography parameters were measured by interferometry (n=5). Elastic modulus (E) and Vickers hardness (VH) were determined by microhardness dynamic indentation (n=5). Diametral tensile strength (DTS) was determined at 0.5 mm/min (n=5). Shade matching was evaluated by using the Vitapan Classic Shade Guide. The DC, Sa, Ssk, Sku, Sdq, and DTS were statistically analyzed using 2-way ANOVA, followed by the Tukey HSD tests. E and VH were statistically analyzed by 3-way ANOVA, followed by Tukey HSD tests (α=.05). Shade matching was analyzed with the Kruskal-Wallis test.DC values were similar for the immediate evaluation and after 24 hours, regardless of the composite resin (P.05). All surface topography parameters increased significantly after the acidic drinks and were greatest for Coca-Cola. The E, VH, and DTS decreased significantly for all composite resins tested (P.05). Acai juice and red wine produced more surface staining than Coca-Cola.Acidic drinks negatively influenced the physical and mechanical properties of conventional and bulk-fill composite resins.

SUMMARY Objectives: To compare radiopacity and porosity as expressed by the presence of voids in restorations carried out using bulk-fill and incremental filling techniques to restore large mesio-occlusal-distal (MOD) cavities. Methods: Fifty-five molars with MOD preparations were incrementally filled with Filtek Z-350XT (Z350XT) or bulk-fill composite: Filtek Bulk Fill/Z-350XT (FBF/Z350XT), Venus Bulk Fill/Charisma Diamond (VBF/CHA), SDR/Esthet-X HD (SDR/EST-X), Tetric EvoCeram Bulk Fill (TEC). Digital radiographic images (Vistascan scanner) were taken of restored molars and analyzed at the gingival and isthmus floors. Radiodensity measurements were performed using standardized points symmetrically distributed over each region of composite and tooth structure. Three calibrated evaluators visually assessed the presence of voids. Confidence intervals were calculated, and data were analyzed using analysis of variance and χ2 tests. Results: TEC and VBF/CHA showed significantly higher radiodensities, while the lowest values were observed for FBF/Z350XT and Z350XT. Radiodensity at the cervical regions tended to be greater than that found at the isthmus floor. The lowest incidence of voids was found for VBF/CHA, whereas the incremental insertion technique resulted in the highest rate of voids. Conclusion: Bulk-fill composite resin demonstrated an adequate level of radiodensity and a reduced presence of voids compared with the incremental filling technique.

SUMMARY Objectives: To evaluate the effect of flowable bulk-fill or conventional composite resin on bond strength and stress distribution in flat or mesio-occlusal-distal (MOD) cavity preparations using the microtensile bond strength (μTBS) test. Methods: Forty human molars were divided into two groups and received either standardized MOD or flat cavity preparations. Restorations were made using the conventional composite resin Z350 (Filtek Z350XT, 3M-ESPE, St Paul, MN, USA) or flowable bulk-fill (FBF) composite resin (Filtek Bulk Fill Flowable, 3M-ESPE). Postgel shrinkage was measured using the strain gauge technique (n=10). The Z350 buildup was made in two increments of 2.0 mm, and the FBF was made in a single increment of 4.0 mm. Six rectangular sticks were obtained for each tooth, and each section was used for μTBS testing at 1.0 mm/min. Polymerization shrinkage was modeled using postgel shrinkage data. The μTBS data were analyzed statistically using a two-way analysis of variance (ANOVA), and the postgel shrinkage data were analyzed using a one-way ANOVA with Tukey post hoc test. The failure modes were analyzed using a chi-square test (α=0.05). Results: Our results show that both the type of cavity preparation and the composite resin used affect the bond strength and stress distribution. The Z350 composite resin had a higher postgel shrinkage than the FBF composite resin. The μTBS of the MOD preparation was influenced by the type of composite resin used. Irrespective of composite resin, flat cavity preparations resulted in higher μTBS than MOD preparations (p Conclusions: FBF composite resin had lower shrinkage stress than Z350 conventional composite resin. The μTBS of the MOD preparation was influenced by the composite resin type. Flat cavity preparations had no influence on stress and μTBS. However, for MOD preparation, composite resin with higher shrinkage stress resulted in lower μTBS values.

SUMMARY Objectives: To analyze the effect of pulp-capping materials and resin composite light activation on strain and temperature development in the pulp and on the interfacial integrity at the pulpal floor/pulp-capping materials in large molar class II cavities. Methods: Forty extracted molars received large mesio-occlusal-distal (MOD) cavity bur preparation with 1.0 mm of dentin remaining at the pulp floor. Four pulp-capping materials (self-etching adhesive system, Clearfil SE Bond [CLE], Kuraray), two light-curing calcium hydroxide cements (BioCal [BIO], Biodinâmica, and Ultra-Blend Plus [ULT], Ultradent), and a resin-modified glass ionomer cement– (Vitrebond [VIT], 3M ESPE) were applied on the pulpal floor. The cavities were incrementally restored with resin composite (Filtek Z350 XT, 3M ESPE). Thermocouple (n=10) and strain gauge (n=10) were placed inside the pulp chamber in contact with the top of the pulpal floor to detect temperature changes and dentin strain during light curing of the pulp-capping materials and during resin composite restoration. Exotherm was calculated by subtracting postcure from polymerization temperature (n=10). Interface integrity at the pulpal floor was investigated using micro-CT (SkyScan 1272, Bruker). The degree of cure of capping materials was calculated using the Fourier transform infrared and attenuated total reflectance cell. Data were analyzed using one-way analysis of variance followed by the Tukey test (α=0.05). Results: Pulpal dentin strains (μs) during light curing of CLE were higher than for other pulp-capping materials (p Conclusions: Light curing of pulp-capping materials caused deformation of pulpal dentin and increased pulpal temperature in large MOD cavities. Shrinkage of the resin composite restoration caused debonding of BIO from the pulpal floor.

Objectives: To evaluate the effects of direct composite resin without a post or with one or two fiberglass posts on the restoration of severely compromised endodontically treated molars. Methods and Materials: Forty-five molars with 2 mm of “remaining tooth structure” were divided into three groups: Wfgp, restored with Filtek Z350XT without a fiberglass post; 1fgp, restored with Z350XT with one fiberglass post in the distal root canal; and 2fgp, restored with Z350XT with two fiberglass posts, one in the distal root canal and the other in the mesial-buccal root canal. The teeth were load cycled. Tooth remaining strain was measured using strain gauges (n=10) at two moments: TrSt-100 N, during 100 N occlusal loading, and TrSt-Fr, at fracture load. Fracture resistance was calculated, and fracture mode was classified. The elastic modulus and Vickers hardness were calculated using dynamic indentation (n=5). Stress distribution was analyzed by three-dimensional finite element analysis. Results: The use of two fiberglass posts resulted in lower fracture resistance than was noted in the groups with one fiberglass post and without fiberglass posts. The lingual surface of the remaining tooth had higher strain values than the buccal surface, regardless of the restorative technique and moment of evaluation. The absence of a fiberglass post resulted in significantly higher strain values and more irreparable fracture modes than were noted in the other groups. The use of one fiberglass post had a better strain/fracture resistance ratio. Stresses were concentrated in the occlusal portion of the post and in the furcation region. The presence of one fiberglass post resulted in better stress distribution in the entire distal root dentin, reducing stress on the critical areas. Conclusions: The use of one fiberglass post for restoring molars with direct composite resin resulted in higher fracture resistance than did the use of two fiberglass posts; it also resulted in better tooth remaining strain and stress distribution and more reparable fracture modes than were seen in the group without a fiberglass post.

SUMMARYNoncarious cervical lesions (NCCLs) are characterized by a loss of dental structure at the cementoenamel junction (CEJ) caused by stress, biocorrosion, and attrition. Variations in occlusal loading can promote different stress and strain patterns on the CEJ. Restoration of NCCLs is part of lesion management; however, there is still no conclusive restorative protocol for NCCLs. This study aimed to evaluate the stress and strain distribution of maxillary premolars with NCCLs according to three factors: 1) restorative technique; 2) direction of occlusal loading; and 3) mechanical fatigue. Three-dimensional (3D) finite element analysis (FEA) and strain gauge testing were used to assess stress and strain, respectively. 3D-FEA orthotropic, linear, and elastic models were generated: sound tooth (SO); unrestored NCCL; or NCCL restored with glass ionomer; flowable composite resin; nanofilled composite resin (CR); lithium disilicate ceramic; and nanofilled composite resin core associated with a lithium disilicate laminate (CL). A 150-N compressive static load was applied in two conditions: axially in both cusps (Al); and at a 45° angle to the long axis of the tooth applied to the palatine cusp (Ol). For the experimental tests, specimens were treated as described previously, and one strain gauge was attached to the buccal surface of each tooth to record tooth strains before and after cyclic loading (200,000 cycles, 50 N). FEA showed that the association of NCCL and Ol resulted in higher stress values. CR and CL restorations showed the closest biomechanical behavior to SO for both loading types. Loaded Al or Ol specimens showed higher strain values after mechanical fatigue. Lower stress and strain were observed with Al when compared with Ol. The restoration of NCCLs with composite resin only or associated with ceramic laminates seems to be the best approach because the results for those groups were similar in biomechanical behaviors to sound teeth.

SUMMARY Objective: The aim of this study was to investigate the effect of delayed photo-activation on elastic modulus, Knoop hardness, and post-gel shrinkage of dual cure resin cements and how this affects residual shrinkage stresses in posterior teeth restored with ceramic inlays. Methods and Materials: Four self-adhesive (RelyX Unicem, 3M ESPE; GCem, GC; MonoCem, Shofu; and seT, SDI) and two conventional (RelyX ARC, 3M ESPE; and AllCem, FGM) dual cure resin cements for cementing posterior ceramic inlays were tested. Strain gauge and indentation tests were used to measure the post-gel shrinkage (Shr), elastic modulus (E), and Knoop hardness (KHN) when photo-activated immediately and 3 and 5 minutes after placement (n=10). Shr, E, and KHN results were analyzed using two-way analysis of variance followed by Tukey honestly significant difference post hoc tests (α=0.05). The experimentally determined properties were applied in a finite element analysis of a leucite ceramic inlay (Empress CAD, Ivoclar Vivadent) cemented in a premolar. Modified von Mises stresses were evaluated at the occlusal margins and cavity floor. Results: Shr, E, and KHN varied significantly among the resin cements (p Conclusions: KHN and E values varied among the different resin cements. Residual shrinkage stress levels decreased with increasing photo-activation delay with all resin cements.