1. Optimization of Fermentation Conditions for Cell Envelope Proteinase Produced by Lactobacillus Plantarum LP69
- Author
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Zemin Liu, Guanli Du, Chi Zhang, Xiangyun Li, Ni Lei, Jili Cao, and He Chen
- Subjects
0303 health sciences ,030309 nutrition & dietetics ,Chemistry ,food and beverages ,04 agricultural and veterinary sciences ,TP368-456 ,lactobacillus plantarum ,040401 food science ,Food processing and manufacture ,Industrial and Manufacturing Engineering ,03 medical and health sciences ,0404 agricultural biotechnology ,Biochemistry ,fermentation optimization ,Fermentation ,orthogonal experiment ,Cell envelope ,cell-envelope proteinases ,Food Science - Abstract
Cell-envelope proteinases (CEPs) can hydrolyze casein into functional peptides, which is beneficial to the health of the host. The single factor experiment screened out that the optimal conditions for CEP production by Lactobacillus plantarum LP69 were 37°C, 20h, initial pH of 7, and optimal inoculation amount of 5%. The best conditions for this experiment were obtained by orthogonal experiment: time 22h, temperature 39°C, initial pH value of 6, and inoculation amount of 5%. Under this culture condition, the target protease activity of Lactobacillus plantarum LP69 reached 22.31±0.82U/mL, the protein content was 19.07±0.36mg/mL, and the specific activity was 1.17±0.06U/mg. The specific activity significantly increased by 15.8% compared with the control (p
- Published
- 2021