1. Prolonged DNA hydrolysis in water: A study on DNA stability
- Author
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Barbara Bertoglio, Carolina Cantoni, Francesca Procopio, Carlo Previderè, Solange Sorçaburu-Cigliero, Irena Zupanič Pajnič, Paola Pitacco, Elisa Recchia, Paolo Fattorini, Pierangela Grignani, Giorgio Marrubini, Serena Bonin, Fattorini, P, Marrubini, G, Bonin, S, Bertoglio, B, Grignani, P, Recchia, E, Pitacco, P, Procopio, F, Cantoni, C, Pajnič, Iz, Sorçaburu-Cigliero, S, and Previdere, C.
- Subjects
0301 basic medicine ,Degraded samples ,Multidisciplinary ,Chromatography ,Parafilm ,DNA hydrolysis ,DNA quantification ,lcsh:Computer applications to medicine. Medical informatics ,qPCR ,DNA hydrolysi ,03 medical and health sciences ,Hydrolysis ,chemistry.chemical_compound ,030104 developmental biology ,chemistry ,DNA stability ,Ultrapure water ,lcsh:R858-859.7 ,Degraded sample ,lcsh:Science (General) ,DNA ,lcsh:Q1-390 - Abstract
This work provides a protocol for the in vitro production of damaged DNA samples. In particular, heat-mediated hydrolysis of the samples at 70 °C in ultrapure water was performed in 1.7 mL Eppendorf tubes sealed by Parafilm for 0–36 h. The chemical/physical features of the resulting samples are described. After normalization of the qPCR data, these were compared with those obtained from samples treated for 0–10 h in a previous study. Keywords: qPCR, Degraded samples, DNA quantification, DNA hydrolysis
- Published
- 2018