Prolonged DNA hydrolysis in water: A study on DNA stability

Authors :: Barbara Bertoglio
Carolina Cantoni
Francesca Procopio
Carlo Previderè
Solange Sorçaburu-Cigliero
Irena Zupanič Pajnič
Paola Pitacco
Elisa Recchia
Paolo Fattorini
Pierangela Grignani
Giorgio Marrubini
Serena Bonin
Fattorini, P
Marrubini, G
Bonin, S
Bertoglio, B
Grignani, P
Recchia, E
Pitacco, P
Procopio, F
Cantoni, C
Pajnič, Iz
Sorçaburu-Cigliero, S
Previdere, C.
Source :: Data in Brief, Vol 20, Iss, Pp 1237-1243 (2018)
Publication Year :: 2018
Abstract: This work provides a protocol for the in vitro production of damaged DNA samples. In particular, heat-mediated hydrolysis of the samples at 70 °C in ultrapure water was performed in 1.7 mL Eppendorf tubes sealed by Parafilm for 0–36 h. The chemical/physical features of the resulting samples are described. After normalization of the qPCR data, these were compared with those obtained from samples treated for 0–10 h in a previous study. Keywords: qPCR, Degraded samples, DNA quantification, DNA hydrolysis

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