1. Pore-forming toxins as tools for polymer analytics: From sizing to sequencing
- Author
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Gerhard Baaken, Ekaterina Zaitseva, Manuela Pastoriza-Gallego, Jan C. Behrends, Hadjer Ouldali, Tobias Ensslen, Mazdak Afshar Bakshloo, Fabien Piguet, Monasadat Talarimoghari, Abdelghani Oukhaled, Laboratoire Analyse, Modélisation et Matériaux pour la Biologie et l'Environnement (LAMBE - UMR 8587), and Université d'Évry-Val-d'Essonne (UEVE)-Institut de Chimie du CNRS (INC)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-CY Cergy Paris Université (CY)
- Subjects
chemistry.chemical_classification ,0303 health sciences ,Resistive touchscreen ,Analyte ,Materials science ,030303 biophysics ,Nanotechnology ,Polymer ,Sizing ,03 medical and health sciences ,Nanopore ,chemistry.chemical_compound ,chemistry ,Molecule ,[PHYS.PHYS.PHYS-CHEM-PH]Physics [physics]/Physics [physics]/Chemical Physics [physics.chem-ph] ,Nanopore sequencing ,Ethylene glycol ,ComputingMilieux_MISCELLANEOUS - Abstract
We report here on the nanopore resistive pulse sensing (Np-RPS) method, involving pore-forming toxins as tools for polymer analytics at single molecule level. Np-RPS is an electrical method for the label-free detection of single molecules. A molecule interacting with the pore causes a change of the electrical resistance of the pore, called a resistive pulse, associated with a measurable transient current blockade. The features of the blockades, in particular their depth and duration, contain information on the molecular properties of the analyte. We first revisit the history of Np-RPS, then we discuss the effect of the configuration of the molecule/nanopore interaction on the molecular information that can be extracted from the signal, illustrated in two different regimes that either favor molecular sequencing or molecular sizing. Specifically, we focus on the sizing regime and on the use of two different pore-forming toxins, staphylococcal α-hemolysin (αHL) and aerolysin (AeL) nanopores, for the characterization of water-soluble polymers (poly-(ethylene glycol), (PEG)), homopeptides, and heteropeptides. We discuss how nanopore sizing of polymers could be envisioned as a new approach for peptide/protein sequencing.
- Published
- 2021