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Electrophysiology on Channel-Forming Proteins in Artificial Lipid Bilayers: Next-Generation Instrumentation for Multiple Recordings in Parallel
- Source :
- Patch Clamp Electrophysiology ISBN: 9781071608173
- Publication Year :
- 2020
- Publisher :
- Springer US, 2020.
-
Abstract
- Artificial lipid bilayers have been used for several decades to study channel-forming pores and ion channels in membranes. Until recently, the classical two-chamber setups have been primarily used for studying the biophysical properties of pore forming proteins. Within the last 10 years, instruments for automated lipid bilayer measurements have been developed and are now commercially available. This chapter focuses on protein purification and reconstitution of channel-forming proteins into lipid bilayers using a classic setup and on the commercially available systems, the Orbit mini and Orbit 16.
- Subjects :
- 0303 health sciences
03 medical and health sciences
Electrophysiology
Membrane
Materials science
Nanotechnology
02 engineering and technology
Instrumentation (computer programming)
021001 nanoscience & nanotechnology
0210 nano-technology
Lipid bilayer
Ion channel
030304 developmental biology
Subjects
Details
- ISBN :
- 978-1-07-160817-3
- ISBNs :
- 9781071608173
- Database :
- OpenAIRE
- Journal :
- Patch Clamp Electrophysiology ISBN: 9781071608173
- Accession number :
- edsair.doi...........704a2a2a539f3314f369caebb9fe5200