1. Inflammation, vitamin D and dendritic cell precursors in chronic kidney disease
- Author
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Stephanie Titze, Sybille Franke, Anna Koettgen, Matthias Schmid, Barbara Bärthlein, Martin Busch, Daniel Kretzschmar, Atilla Yilmaz, Jennifer Nadal, Katharina Paul, and Gunter Wolf
- Subjects
Male ,medicine.medical_specialty ,Myeloid ,Immunology ,030232 urology & nephrology ,Renal function ,Cell Count ,Inflammation ,Comorbidity ,030204 cardiovascular system & hematology ,Flow cytometry ,03 medical and health sciences ,0302 clinical medicine ,Risk Factors ,Internal medicine ,Diabetes mellitus ,Vitamin D and neurology ,medicine ,Humans ,Immunology and Allergy ,Renal Insufficiency, Chronic ,Vitamin D ,Aged ,medicine.diagnostic_test ,business.industry ,Stem Cells ,Dendritic Cells ,Original Articles ,Dendritic cell ,Middle Aged ,medicine.disease ,C-Reactive Protein ,Endocrinology ,medicine.anatomical_structure ,Female ,medicine.symptom ,business ,Biomarkers ,Kidney disease - Abstract
Summary Decreased blood dendritic cell precursors (DCP) count is linked with atherosclerotic disease, while reduction of circulating DCP is also seen in patients with chronic kidney disease (CKD). As poor vitamin D status could be linked to a compromised innate immune response, we hypothesized that vitamin D status might be involved in the decrease in circulating DCP in CKD. Moreover, the potential role of inflammation was considered. Circulating myeloid (mDCP), plasmacytoid (pDCP) and total DCP (tDCP) were analysed using flow cytometry in 287 patients with CKD stage 3. Serum 25(OH)D and 1,25(OH)2D levels were measured using enzyme-linked immunosorbent assays (ELISA), interleukin (IL)-6, IL-10 and tumour necrosis factor (TNF)-α using cytometric bead array, C-reactive protein (CRP) using a high-sensitivity (hs) ELISA. Contrary to our hypothesis, there was no association between vitamin D levels and DCP, although their number was decreased significantly in CKD (P
- Published
- 2016