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57 results on '"Laurence Raehm"'

1. Synthesis of Cyclen‐Functionalized Ethenylene‐Based Periodic Mesoporous Organosilica Nanoparticles and Metal‐Ion Adsorption Studies

Author

Gulaim A. Seisenbaeva, Clarence Charnay, Hao Li, Roser Pleixats, Laurence Raehm, Ani Vardanyan, Jean-Olivier Durand, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Swedish University of Agricultural Sciences (SLU), and Universitat Autònoma de Barcelona (UAB)

Subjects
[CHIM.ORGA]Chemical Sciences/Organic chemistry, Renewable Energy, Sustainability and the Environment, Chemistry, Energy Engineering and Power Technology, 02 engineering and technology, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, 0104 chemical sciences, Biomaterials, chemistry.chemical_compound, Mesoporous organosilica, Adsorption, Cyclen, Transition metal, Desorption, Polymer chemistry, Materials Chemistry, Click chemistry, Nanorod, Other Chemistry Topics, 0210 nano-technology, Selectivity
Abstract

International audience; The preparation of two cyclens both possessing two triethoxysilyl groups through click chemistry is described. These two cyclens were incorporated into bis(triethoxysilyl)ethenylene‐based periodic mesoporous organosilica nanoparticles (PMO NPs) at different proportions of bis(triethoxysilyl)ethenylene/cyclens (90/10, 75/25). The obtained nanorods were analyzed with different techniques and showed high specific surface areas at low proportion of cyclens. The nanorods containing free amino groups of cyclen were then used for Ni(II) and Co(II) removal from model solutions. The kinetics and isotherms of adsorption of Ni(II) and Co(II) were determined, and the materials showed high uptake of metals (up to 3.9 mmol ⋅ g−1). They demonstrated pronounced selectivity in separation of rare earth elements from late transition metals, e. g. Ni(II) and Co(II) by adsorption and even more so by controlled desorption..

Published
2020

2. Organosilica Nanoparticles for Gemcitabine Monophosphate Delivery in Cancer Cells

Author

Saher Rahmani, Jelena Budimir, Morgane Daurat, Yannick Guari, Laurence Raehm, Peter Hesemann, Sébastien Richeter, Clarence Charnay, Roza Bouchal, Magali Gary-Bobo, Nadir Bettache, Alia Akrout, Christophe Nguyen, Jean-Olivier Durand, Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Centre de recherche en Biologie Cellulaire (CRBM), and Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
gemcitabine monophosphate, organosilica nanoparticles, drug delivery, cancer, ionosilica, Renewable Energy, Sustainability and the Environment, Chemistry, Energy Engineering and Power Technology, Nanoparticle, Cancer, 02 engineering and technology, 010402 general chemistry, 021001 nanoscience & nanotechnology, medicine.disease, 01 natural sciences, Gemcitabine, 0104 chemical sciences, Biomaterials, Cancer cell, Drug delivery, Materials Chemistry, medicine, Cancer research, [CHIM.COOR]Chemical Sciences/Coordination chemistry, 0210 nano-technology, ComputingMilieux_MISCELLANEOUS, medicine.drug
Abstract

Organosilica nanoparticles hold great promise for nanomedicine applications. These nanoparticles are synthesized from polytrialkoxysilylated precursors without any silica source. In this work we present two kinds of organosilica nanoparticles with either amine or ammonium walls constituting their structure. Both types of nanoparticles are very efficient for gemcitabine monophosphate delivery, a small hydrophilic anticancer drug whose encapsulation is still a challenge. The nanoparticles are endocytosed by MCF-7 breast cancer cells as monitored by confocal microscopy. They are efficient and lead to 60% cancer cell death.

Published
2019

3. Synthesis of triethoxysilylated cyclen derivatives, grafting on magnetic mesoporous silica nanoparticles and application to metal ion adsorption

Author

Erwan Oliviero, Jean-Olivier Durand, Roser Pleixats, Clarence Charnay, Gulaim A. Seisenbaeva, Hao Li, Laurence Raehm, Mathilde Ménard, Ani Vardanyan, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Swedish University of Agricultural Sciences (SLU), Departament de Química [Barcelona] (UAB), and Universitat Autònoma de Barcelona (UAB)

Subjects
Solid-state chemistry, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Chemistry, General Chemical Engineering, Metal ions in aqueous solution, Nanoparticle, 02 engineering and technology, General Chemistry, Mesoporous silica, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, 0104 chemical sciences, chemistry.chemical_compound, Adsorption, Cyclen, Materials Chemistry, Click chemistry, Surface modification, 0210 nano-technology, Nuclear chemistry
Abstract

International audience; The synthesis through click chemistry of triethoxysilylated cyclen derivative-based ligands is described. Different methods were used such as the copper catalyzed Huisgen's reaction, or thiol-ene reaction for the functionalization of the cyclen scaffold with azidopropyltriethoxysilane or mercaptopropyltriethoxysilane, respectively. These ligands were then grafted on magnetic mesoporous silica nanoparticles (MMSN) for extraction and separation of Ni(II) and Co(II) metal ions from model solutions. The bare and ligand-modified MMSN materials revealed high adsorption capacity (1.0-2.13 mmol g À1) and quick adsorption kinetics, achieving over 80% of the total capacity in 1-2 hours.

Published
2021

4. Nanodiamonds for bioapplications, recent developments

Author

Rabah Boukherroub, Nicolas Bondon, Laurence Raehm, Clarence Charnay, Jean-Olivier Durand, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM), and Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Computer science, Biomedical Engineering, Antineoplastic Agents, Nanotechnology, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Nanodiamonds, Drug Delivery Systems, Anti-Infective Agents, Drug Development, Animals, Humans, [CHIM]Chemical Sciences, General Materials Science, Particle Size, Biomedicine, Drug Carriers, Wound Healing, business.industry, General Chemistry, General Medicine, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, 0104 chemical sciences, 3. Good health, Nanomedicine, 0210 nano-technology, business
Abstract

International audience; The world of biomedical research is in constant evolution, requiring more and more conditions and norms through pre-clinic and clinic studies. Nanodiamonds (NDs) with exceptional optical, thermal and mechanical properties emerged on the global scientific scene and recently gained more attention in biomedicine and bioanalysis fields. Many problematics have been deliberated to better understand their in vitro and in vivo efficiency and compatibility. Light was shed on their synthesis, modification and purification steps, as well as particle size and surface properties in order to find the most suitable operating conditions. In this review, we present the latest advances of NDs use in bioapplications. A large variety of subjects including anticancer and antimicrobial systems, wound healing and tissue engineering management tools, but also bioimaging and labeling probes are tackled. The key information resulting from these recent works were evidenced to make an overview of the potential features of NDs, with a special look on emerging therapeutic and diagnosis combinations.

Published
2020

5. Fluorescent periodic mesoporous organosilica nanoparticles dual-functionalized via click chemistry for two-photon photodynamic therapy in cells

Author

Marie Maynadier, Laurence Raehm, Jean-Olivier Durand, Xavier Cattoën, Marcel Garcia, Maxime Klausen, Sébastien Picard, Dina Aggad, Guillaume Clermont, Chiara Mauriello Jimenez, Mireille Blanchard-Desce, Jonas G. Croissant, Emilie Genin, Michel Wong Chi Man, Magali Gary-Bobo, Olivier Mongin, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1 (UB)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Optique et Matériaux (NEEL - OPTIMA), Institut Néel (NEEL), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Optique et Matériaux (OPTIMA )

Subjects
Fluorophore, Materials science, Singlet oxygen, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Biomedical Engineering, Nanoparticle, 02 engineering and technology, General Chemistry, General Medicine, 010402 general chemistry, 021001 nanoscience & nanotechnology, Photochemistry, 01 natural sciences, Fluorescence, 0104 chemical sciences, chemistry.chemical_compound, Mesoporous organosilica, chemistry, Click chemistry, General Materials Science, Photosensitizer, 0210 nano-technology, Mesoporous material, ComputingMilieux_MISCELLANEOUS
Abstract

The synthesis of ethenylene-based periodic mesoporous organosilica nanoparticles for two-photon imaging and photodynamic therapy of breast cancer cells is described. A dedicated two-photon absorbing fluorophore possessing four triethoxysilyl groups and having large two-photon absorption in the near IR region, and azidopropyltriethoxysilane were incorporated into the structure. The mesoporous nanoparticles of 100 nm diameter were further functionalized by means of click chemistry with a propargylated fluorescent bromo-quinoline photosensitizer able to generate singlet oxygen. The photophysical properties and two-photon absorption properties of the nanoparticles were investigated evidencing complementary contribution of the two dyes. Both dyes contribute to the two-photon absorption response of the mesoporous nanoparticles while efficient FRET from the two-photon fluorophore to the quinoline sensitizer is observed. The dual-functionalized nanoparticles were incubated with MCF-7 breast cancer cells. Two-photon confocal imaging demonstrated the endocytosis of the nanoparticles within cancer cells. Moreover, brief two-photon irradiation (3 scans of 1.57 s) at 760 nm at high laser power (3 W) was shown to induce 40% of cancer cell death demonstrating the potential of the dual-functionalized mesoporous organosilica nanoparticles for two-photon photodynamic therapy.

Published
2020

6. Influence of the synthetic method on the properties of two-photon-sensitive mesoporous silica nanoparticles

Author

Marie Maynadier, Laurence Raehm, Clarence Charnay, Michel Wong Chi Man, Vanja Stojanovic, Xavier Cattoën, Magali Gary-Bobo, Olivier Mongin, Jonas G. Croissant, Jean-Olivier Durand, Mireille Blanchard-Desce, Marcel Garcia, Vincent Hugues, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1 (UB)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Optique et Matériaux (NEEL - OPTIMA), Institut Néel (NEEL), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), NanoMedSyn (NMS), ANR-10-NANO-0022,MECHANANO,Nanomachines mécanisées pour l'activation à deux photons(2010), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Optique et Matériaux (OPTIMA), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), and NanoMedSyn

Subjects
cross-section, Materials science, Mesoporous silica nanoparticles, Biomedical Engineering, Nanoparticle, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, chemistry.chemical_compound, Two-photon excitation microscopy, Bromide, Organic chemistry, General Materials Science, Photosensitizer, Porosity, two-photon, Aqueous medium, [CHIM.ORGA]Chemical Sciences/Organic chemistry, imaging, General Chemistry, General Medicine, Mesoporous silica, 021001 nanoscience & nanotechnology, 0104 chemical sciences, chemistry, Chemical engineering, Spatial dispersion, cancer cells, 0210 nano-technology
Abstract

International audience; Herein we report the modulation of the properties of mesoporous silica nanoparticles (NPs) via various synthetic approaches. Three types of elaborations were compared, one in aqueous media at 25 °C, and the other two at 80 °C in water or in a water–ethanol mixture. For all these methods, an alkoxysilylated two-photon photosensitizer (2PS) was co-condensed with tetraethylorthosilicate (TEOS) in the presence of cetyltrimethylammonium bromide (CTAB), leading to five two-photon-sensitive mesoporous silica (M2PS) NPs. The M2PS NP porous structure could be tuned from radial to worm-like and MCM-41 types of organization. Besides, the 2PS precursor spatial dispersion was found to be highly dependent on both the 2PS initial concentration and the elaboration process. As a result, two-photon properties were modulated by the choice of the synthesis, the best results being found in aqueous media at 25 or 80 °C. Finally, the M2PS NPs were used for in vitro two-photon imaging of cancer cells.

Published
2020

7. Periodic Mesoporous Organosilica Nanoparticles with BOC Group, towards HIFU Responsive Agents

Author

Roser Pleixats, Clarence Charnay, Chantal Pichon, Laurence Raehm, Hao Li, Anthony Delalande, Jean-Olivier Durand, Carolina Gascó, Patrick Midoux, Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Department of Chemistry, and Universitat Autònoma de Barcelona (UAB)

Subjects
CO2, Formic Acid Esters, Contrast Media, Pharmaceutical Science, Nanoparticle, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Article, Analytical Chemistry, lcsh:QD241-441, lcsh:Organic chemistry, Drug Discovery, Humans, Molecule, cancer, [CHIM]Chemical Sciences, Organosilicon Compounds, Physical and Theoretical Chemistry, Cancer, Drug Carriers, Nanotubes, Chemistry, Organic Chemistry, periodic mesoporous organosilica nanoparticles, HIFU, Carbon Dioxide, Silicon Dioxide, 021001 nanoscience & nanotechnology, Combinatorial chemistry, 3. Good health, 0104 chemical sciences, Mesoporous organosilica, Nanomedicine, Polymerization, Chemistry (miscellaneous), Click chemistry, Nanoparticles, Molecular Medicine, Click Chemistry, Nanorod, Periodic mesoporous organosilica nanoparticles, 0210 nano-technology, Mesoporous material, Porosity
Abstract

Periodic Mesoporous Organosilica Nanoparticles (PMONPs) are nanoparticles of high interest for nanomedicine applications. These nanoparticles are not composed of silica (SiO2). They belong to hybrid organic&ndash, inorganic systems. We considered using these nanoparticles for CO2 release as a contrast agent for High Intensity Focused Ultrasounds (HIFU). Three molecules (P1&ndash, P3) possessing two to four triethoxysilyl groups were synthesized through click chemistry. These molecules possess a tert-butoxycarbonyl (BOC) group whose cleavage in water at 90&ndash, 100 &deg, C releases CO2. Bis(triethoxysilyl)ethylene E was mixed with the molecules Pn (or not for P3) at a proportion of 90/10 to 75/25, and the polymerization triggered by the sol-gel procedure led to PMONPs. PMONPs were characterized by different techniques, and nanorods of 200&ndash, 300 nm were obtained. These nanorods were porous at a proportion of 90/10, but non-porous at 75/25. Alternatively, molecules P3 alone led to mesoporous nanoparticles of 100 nm diameter. The BOC group was stable, but it was cleaved at pH 1 in boiling water. Molecules possessing a BOC group were successfully used for the preparation of nanoparticles for CO2 release. The BOC group was stable and we did not observe release of CO2 under HIFU at lysosomal pH of 5.5. The pH needed to be adjusted to 1 in boiling water to cleave the BOC group. Nevertheless, the concept is interesting for HIFU theranostic agents.

Published
2020

8. Preparation and characterization of novel mixed periodic mesoporous organosilica nanoparticles

Author

Hao Li, Clarence Charnay, Roser Pleixats, Laurence Raehm, Jean-Olivier Durand, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), and Universitat Autònoma de Barcelona (UAB)

Subjects
Thermogravimetric analysis, Materials science, Nanoparticle, 02 engineering and technology, 010402 general chemistry, sol-gel process, lcsh:Technology, 01 natural sciences, Article, Nanomaterials, Dynamic light scattering, 5dialkoxybenzoates, [CHIM]Chemical Sciences, General Materials Science, Sol-gel process, lcsh:Microscopy, lcsh:QC120-168.85, Sol-gel, disilylated tert-butyl 3,5-dialkoxybenzoates, lcsh:QH201-278.5, lcsh:T, periodic mesoporous organosilica nanoparticles, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Mesoporous organosilica, Disilylated tert -butyl 3,5-dialkoxybenzoates, lcsh:TA1-2040, disilylated tert-butyl 3, lcsh:Descriptive and experimental mechanics, Nanorod, lcsh:Electrical engineering. Electronics. Nuclear engineering, Periodic mesoporous organosilica nanoparticles, lcsh:Engineering (General). Civil engineering (General), 0210 nano-technology, Mesoporous material, lcsh:TK1-9971, Nuclear chemistry
Abstract

We report herein the preparation of mixed periodic mesoporous organosilica nanoparticles (E-Pn 75/25 and 90/10 PMO NPs) by sol-gel co-condensation of E-1,2-bis(triethoxysilyl)ethylene ((E)-BTSE or E) with previously synthesized disilylated tert-butyl 3,5-dialkoxybenzoates bearing either sulfide (precursor P1) or carbamate (precursor P2) functionalities in the linker. The syntheses were performed with cetyltrimethylammonium bromide (CTAB) as template in the presence of sodium hydroxide in water at 80 &deg, C. The nanomaterials have been characterized by Transmission Electron Microscopy (TEM), nitrogen-sorption measurements (BET), Dynamic Light Scattering (DLS), zeta-potential, Thermogravimetric Analysis (TGA), FTIR, 13C CP MAS NMR and small angle X-ray diffraction (p-XRD). All the nanomaterials were obtained as mesoporous rodlike-shape nanoparticles. Remarkably, E-Pn 90/10 PMO NPs presented high specific surface areas ranging from 700 to 970 m2g-1, comparable or even higher than pure E PMO nanorods. Moreover, XRD analyses showed an organized porosity for E-P1 90/10 PMO NPs typical for a hexagonal 2D symmetry. The other materials showed a worm-like mesoporosity.

Published
2020

9. Encapsulation of Upconversion Nanoparticles in Periodic Mesoporous Organosilicas

Author

Clarence Charnay, Christophe Nguyen, Dina Aggad, Manuel Ocaña, Magali Gary-Bobo, Rachid Mahiou, Lamiaa M. A. Ali, Erwan Oliveiro, Damien Boyer, Ana Isabel Becerro Nieto, Jean-Olivier Durand, Laurence Raehm, Nadège Francolon, Daniel González-Mancebo, Chiara Mauriello Jimenez, Saher Rahmani, Institut de Chimie de Clermont-Ferrand - Clermont Auvergne (ICCF), Sigma CLERMONT (Sigma CLERMONT)-Université Clermont Auvergne (UCA)-Centre National de la Recherche Scientifique (CNRS), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Instituto de Ciencia de Materiales de Sevilla (ICMSE), Universidad de Sevilla / University of Sevilla-Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Institut de Chimie de Clermont-Ferrand (ICCF), SIGMA Clermont (SIGMA Clermont)-Institut de Chimie du CNRS (INC)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Agencia Estatal de Investigación (España), Agence Nationale de la Recherche (France), Ministerio de Ciencia, Innovación y Universidades (España), European Commission, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Universidad de Sevilla-Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), and Clot, Eric

Subjects
Materials science, Condensation polymer, Pharmaceutical Science, Nanoparticle, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Article, Analytical Chemistry, lcsh:QD241-441, Fluorides, Drug Delivery Systems, lcsh:Organic chemistry, TheoryofComputation_ANALYSISOFALGORITHMSANDPROBLEMCOMPLEXITY, [CHIM] Chemical Sciences, Drug Discovery, Technology, Pharmaceutical, cancer, [CHIM]Chemical Sciences, Organosilicon Compounds, Yttrium, Physical and Theoretical Chemistry, Ytterbium, ComputingMilieux_MISCELLANEOUS, Cancer, upconversion, Ethane, Nanotubes, Hydrolysis, Organic Chemistry, periodic mesoporous organosilica nanoparticles, 021001 nanoscience & nanotechnology, Photon upconversion, 0104 chemical sciences, Mesoporous organosilica, Nanomedicine, Chemical engineering, Chemistry (miscellaneous), Drug delivery, Molecular Medicine, Nanoparticles, Nanorod, Periodic mesoporous organosilica nanoparticles, 0210 nano-technology, Mesoporous material, Upconversion, Erbium
Abstract

1) Background: Nanomedicine has recently emerged as a promising field, particularly for cancer theranostics. In this context, nanoparticles designed for imaging and therapeutic applications are of interest. We, therefore, studied the encapsulation of upconverting nanoparticles in mesoporous organosilica nanoparticles. Indeed, mesoporous organosilica nanoparticles have been shown to be very efficient for drug delivery, and upconverting nanoparticles are interesting for near-infrared and X-ray computed tomography imaging, depending on the matrix used. (2) Methods: Two different upconverting-based nanoparticles were synthesized with Yb3+-Er3+ as the upconverting system and NaYF4 or BaLuF5 as the matrix. The encapsulation of these nanoparticles was studied through the sol-gel procedure with bis(triethoxysilyl)ethylene and bis(triethoxysilyl)ethane in the presence of CTAB. (3) Results: with bis(triethoxysilyl)ethylene, BaLuF5: Yb3+-Er3+, nanoparticles were not encapsulated, but anchored on the surface of the obtained mesoporous nanorods BaLuF5: Yb3+-Er3+@Ethylene. With bis(triethoxysilyl)ethane, BaLuF5: Yb3+-Er3+ and NaYF4: Yb3+-Er3+nanoparticles were encapsulated in the mesoporous cubic structure leading to BaLuF5: Yb3+-Er3+@Ethane and NaYF4: Yb3+-Er3+@Ethane, respectively. (4) Conclusions: upconversion nanoparticles were located on the surface of mesoporous nanorods obtained by hydrolysis polycondensation of bis(triethoxysilyl)ethylene, whereas encapsulation occurred with bis(triethoxysilyl)ethane. The later nanoparticles NaYF4: Yb3+-Er3+@Ethane or BaLuF5: Yb3+-Er3+@Ethane were promising for applications with cancer cell imaging or X-ray-computed tomography respectively., This research was funded by ANR NanoptPDT. SR thanks the Erasmus Mundus program for a grant. This work has been supported by the Spanish Ministry of Science, Innovation and Universities (RTI2018-094426-B-I00).

Published
2019

10. Gemcitabine Delivery and Photodynamic Therapy in Cancer Cells via Porphyrin-Ethylene-Based Periodic Mesoporous Organosilica Nanoparticles

Author

Jean-Olivier Durand, Sébastien Richeter, Marie Maynadier, Jonas G. Croissant, Laure Lichon, Makhlouf Boufatit, Marcel Garcia, Laurence Raehm, Chiara Mauriello Jimenez, Danielle Laurencin, Dina Aggad, Soraya Dib, Shahad Alsaiari, Magali Gary-Bobo, and Niveen M. Khashab

Subjects
Materials science, Membrane permeability, medicine.medical_treatment, Energy Engineering and Power Technology, Nanotechnology, Photodynamic therapy, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Gemcitabine Hydrochloride, Biomaterials, chemistry.chemical_compound, Materials Chemistry, medicine, Photosensitizer, Renewable Energy, Sustainability and the Environment, 021001 nanoscience & nanotechnology, Porphyrin, Gemcitabine, 0104 chemical sciences, Mesoporous organosilica, chemistry, Cancer cell, Cancer research, 0210 nano-technology, medicine.drug
Abstract

Gemcitabine hydrochloride is an FDA-approved chemotherapeutic drug used in the treatment of various cancers. Several drawbacks of gemcitabine including its short in vivo half-life of 8-17 min associated with a rapid excretion by the kidneys and its poor membrane permeability have inspired research on a nanodelivery approach. In this study, we report ethylene-based periodic mesoporous organosilica nanoparticles (PMOs) for photodynamic therapy and the autonomous delivery of gemcitabine in cancer cells. Porphyrins were used as photosensitizers and were localized in the walls of the PMOs while a high loading capacity of gemcitabine was observed in the porous structure. Depending on the nature of the photosensitizer, and its aggregation state, we were able to perform one or two-photon photodynamic therapy. Two-photon excited photodynamic therapy combined with gemcitabine delivery led to a synergy and a very efficient cancer cell killing.

Published
2017

11. Porphyrin- or phthalocyanine-bridged silsesquioxane nanoparticles for two-photon photodynamic therapy or photoacoustic imaging

Author

Vefa Ahsen, Dina Aggad, Jean-Luc Coll, Serkan Alpugan, Michel Wong Chi Man, Marcel Garcia, Marie Maynadier, Laurence Raehm, Deniz Kutlu Tarakci, Philippe Maillard, Fabienne Dumoulin, Clarence Charnay, Jean-Olivier Durand, Maxime Henry, Magali Gary-Bobo, Xavier Cattoën, Véronique Josserand, Chiara Mauriello-Jimenez, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Optique et Matériaux (OPTIMA ), Institut Néel (NEEL), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Department of chemistry - Gebze Technical University, Gebze Teknik Üniversitesi [Gebze], Chimie, Modélisation et Imagerie pour la Biologie [Orsay], Institut de Chimie du CNRS (INC)-Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Curie [Paris], and NanoMedSyn

Subjects
Materials science, [CHIM.ORGA]Chemical Sciences/Organic chemistry, medicine.medical_treatment, Nanoparticle, Photodynamic therapy, [CHIM.MATE]Chemical Sciences/Material chemistry, 02 engineering and technology, 010402 general chemistry, 021001 nanoscience & nanotechnology, Photochemistry, 01 natural sciences, Porphyrin, Fluorescence, Silsesquioxane, 0104 chemical sciences, 3. Good health, chemistry.chemical_compound, chemistry, Two-photon excitation microscopy, Excited state, Phthalocyanine, medicine, General Materials Science, 0210 nano-technology, ComputingMilieux_MISCELLANEOUS
Abstract

Porphyrin- or phthalocyanine-bridged silsesquioxane nanoparticles (BSPOR and BSPHT) were prepared. Their endocytosis in MCF-7 cancer cells was shown with two-photon excited fluorescence (TPEF) imaging. With two-photon excited photodynamic therapy (TPE-PDT), BSPOR was more phototoxic than BSPHT, which in contrast displayed a very high signal for photoacoustic imaging in mice.

Published
2017

12. Efficient Photodynamic Therapy of Prostate Cancer Cells through an Improved Targeting of the Cation-Independent Mannose 6-Phosphate Receptor

Author

Marcel Garcia, Jean-Olivier Durand, Marie Maynadier, Elise Bouffard, Laurence Raehm, Khaled El Cheikh, Chiara Mauriello Jimenez, Christophe Nguyen, Alain Morère, Magali Gary-Bobo, Ilaria Basile, Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), and NanoMedSyn

Subjects
Male, mannose 6-phosphate analogues, medicine.medical_treatment, Mannose, Photodynamic therapy, 02 engineering and technology, 010402 general chemistry, Endocytosis, 01 natural sciences, Receptor, IGF Type 2, Catalysis, lcsh:Chemistry, Inorganic Chemistry, chemistry.chemical_compound, Prostate cancer, Cell Line, Tumor, binding affinity, medicine, Humans, [CHIM]Chemical Sciences, Physical and Theoretical Chemistry, Receptor, mesoporous silica nanoparticles, lcsh:QH301-705.5, Molecular Biology, Spectroscopy, Mannosephosphates, Communication, Organic Chemistry, Prostatic Neoplasms, General Medicine, Mesoporous silica, Silicon Dioxide, 021001 nanoscience & nanotechnology, medicine.disease, 0104 chemical sciences, Computer Science Applications, Cation-independent mannose-6 phosphate receptor, lcsh:Biology (General), lcsh:QD1-999, Photochemotherapy, chemistry, photodynamic therapy, Cancer research, Nanoparticles, 0210 nano-technology
Abstract

This article belongs to the Special Issue Insights into Photodynamic TherapyThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).; International audience; The aim of the present work is the development of highly efficient targeting molecules to specifically address mesoporous silica nanoparticles (MSNs) designed for the photodynamic therapy (PDT) of prostate cancer. We chose the strategy to develop a novel compound that allows the improvement of the targeting of the cation-independent mannose 6-phosphate receptor, which is overexpressed in prostate cancer. This original sugar, a dimannoside-carboxylate (M6C-Man) grafted on the surface of MSN for PDT applications, leads to a higher endocytosis and thus increases the efficacy of MSNs. View Full-TextKeywords: mannose 6-phosphate analogues; binding affinity; mesoporous silica nanoparticles; photodynamic therapy

Published
2019

13. Porphyrin‐based bridged silsesquioxane nanoparticles for targeted two‐photon photodynamic therapy of zebrafish xenografted with human tumor

Author

Magali Gary-Bobo, Denis Durand, Makhlouf Boufatit, Soraya Dib, Jean-Olivier Durand, Ahmed Lakrafi, Alain Morère, Sofia Dominguez Gil, Vincent Chaleix, Dina Aggad, Nadir Bettache, Karim Bouchmella, Michel Wong Chi Man, Vincent Sol, Laurence Raehm, Guillaume Hery, Xavier Cattoën, Khaled El Cheikh, Chiara Mauriello Jimenez, Christophe Nguyen, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), CHU Pontchaillou [Rennes], Laboratoire de Chimie des Substances Naturelles (LCSN), Université de Limoges (UNILIM)-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503), Université des Sciences et de la Technologie Houari Boumediene [Alger] (USTHB), Optique et Matériaux (OPTIMA ), Institut Néel (NEEL), and Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])

Subjects
Cancer Research, Porphyrins, medicine.medical_treatment, Nanoparticle, Breast Neoplasms, Photodynamic therapy, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, 02 engineering and technology, [CHIM.INOR]Chemical Sciences/Inorganic chemistry, 010402 general chemistry, 01 natural sciences, Theranostic Nanomedicine, chemistry.chemical_compound, Two-photon excitation microscopy, In vivo, Cell Line, Tumor, medicine, Animals, Humans, Zebrafish, two-photon excitation, Photosensitizing Agents, bridged silsesquioxane nanoparticles, Lasers, human tumor targeting, Original Articles, Silanes, 021001 nanoscience & nanotechnology, Xenograft Model Antitumor Assays, Porphyrin, 3. Good health, 0104 chemical sciences, Microscopy, Fluorescence, Multiphoton, photodynamic therapy, Photochemotherapy, Oncology, chemistry, Injections, Intravenous, Cancer cell, Click chemistry, Biophysics, Nanoparticles, Nanomedicine, Female, 0210 nano-technology
Abstract

International audience; Background: Bridged silsesquioxane nanoparticles (BSNs) recently described represent a new class of nanoparticles exhibiting versatile applications and particularly a strong potential for nanomedicine.Aims: In this work, we describe the synthesis of BSNs from an octasilylated functional porphyrin precursor (PORBSNs) efficiently obtained through a click reaction. These innovative and very small-sized nanoparticles were functionalized with PEG and mannose (PORBSNs-mannose) in order to target breast tumors in vivo. Methods and Results: The structure of these nanoparticles is constituted of porphyrins J aggregates that allow two-photon spatiotemporal excitation of the nanoparticles. The therapeutic potential of such photoactivable nanoparticles was first studied in vitro, in human breast cancer cells in culture and then in vivo on zebrafish embryos bearing human tumors. These animal models were intravenously injected with 5 nL of a solution containing PORBSNs-mannose. An hour and half after the injection of photoactivable and targeted nanoparticles, the tumor areas were excited for few seconds with a two-photon beam induced focused laser. We observed strong tumor size decrease, with the involvement of apoptosis pathway activation.onclusion: We demonstrated the high targeting, imaging, and therapeutic potential of PORBSNs-mannose injected in the blood stream of zebrafish xenografted with human tumors.

Published
2019

14. Large Pore Mesoporous Silica and Organosilica Nanoparticles for Pepstatin A Delivery in Breast Cancer Cells

Author

Laurence Raehm, Laure Lichon, Morgane Daurat, Clarence Charnay, Marcel Garcia, Dina Aggad, Magali Gary-Bobo, Jean-Olivier Durand, Mylene Sejalon, Eric Vivès, Jelena Budimir, Saher Rahmani, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Centre de recherche en Biologie Cellulaire (CRBM), Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), and Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Dispersity, Pharmaceutical Science, Nanoparticle, Peptide, Breast Neoplasms, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Article, pepstatin A, Analytical Chemistry, lcsh:QD241-441, chemistry.chemical_compound, Drug Delivery Systems, lcsh:Organic chemistry, Cell Line, Tumor, Drug Discovery, Pepstatins, Humans, cancer, [CHIM]Chemical Sciences, mesoporous silica nanoparticles, mesoporous organosilica nanoparticles, Physical and Theoretical Chemistry, chemistry.chemical_classification, Chemistry, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Organic Chemistry, Mesoporous silica, 021001 nanoscience & nanotechnology, Silicon Dioxide, 0104 chemical sciences, 3. Good health, Membrane, Chemical engineering, Chemistry (miscellaneous), Cancer cell, Molecular Medicine, Nanomedicine, Nanoparticles, Female, 0210 nano-technology, Porosity, Pepstatin
Abstract

International audience; (1) Background: Nanomedicine has recently emerged as a new area of research, particularly to fight cancer. In this field, we were interested in the vectorization of pepstatin A, a peptide which does not cross cell membranes, but which is a potent inhibitor of cathepsin D, an aspartic protease particularly overexpressed in breast cancer. (2) Methods: We studied two kinds of nanoparticles. For pepstatin A delivery, mesoporous silica nanoparticles with large pores (LPMSNs) and hollow organosilica nanoparticles (HOSNPs) obtained through the sol-gel procedure were used. The nanoparticles were loaded with pepstatin A, and then the nanoparticles were incubated with cancer cells. (3) Results: LPMSNs were monodisperse with 100 nm diameter. HOSNPs were more polydisperse with diameters below 100 nm. Good loading capacities were obtained for both types of nanoparticles. The nanoparticles were endocytosed in cancer cells, and HOSNPs led to the best results for cancer cell killing. (4) Conclusions: Mesoporous silica-based nanoparticles with large pores or cavities are promising for nanomedicine applications with peptides.

Published
2019

15. Degradable Hollow Organosilica Nanoparticles for Antibacterial Activity

Author

Jean-Olivier Durand, Philippe Trens, Saher Rahmani, Karim Bouchmella, Laurence Raehm, Mateus Borba Cardoso, Clarence Charnay, Jelena Budimir, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), University of Göttingen - Georg-August-Universität Göttingen, Laboratório Nacional de Luz Sìncrotron (LNLS), and Centro Nacional de Pesquisa em Energia e Materiais (CNPEM)

Subjects
Chemistry, Methylamine, General Chemical Engineering, Dispersity, Nanoparticle, 02 engineering and technology, General Chemistry, [CHIM.MATE]Chemical Sciences/Material chemistry, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, 010402 general chemistry, 021001 nanoscience & nanotechnology, organosilica nanoparticles, antibacterial activity, 01 natural sciences, 0104 chemical sciences, lcsh:Chemistry, chemistry.chemical_compound, lcsh:QD1-999, 0210 nano-technology, Antibacterial activity, ComputingMilieux_MISCELLANEOUS, Nuclear chemistry
Abstract

The synthesis of hollow organosilica nanoparticles is described from bis triethoxysilylpropyldisulfide and bis(trimethoxylsilylpropyl)methylamine. The nanoparticles were monodisperse with a mean diameter of 90 nm, and the adsorption−desorption properties of these nanoparticles were investigated with nitrogen at 77 K, n-hexane, and water. The nanoparticles were nonporous at 77 K as shown by N2 sorption analyses. n-Hexane interacted reversibly through physisorption with the nanoparticles, whereas water showed a strong nonreversible interaction, probably because of chemisorption with nitrogen atom sites. Amoxicillin antibiotic was loaded with these nanoparticles and the loading capacity was 30%. Ag+ ions were also adsorbed with amoxicillin, and the multifunctional nanoparticles were efficient in inhibiting bacterial growth with a synergy between the antibiotic delivery and Ag+ release.

Published
2019

16. Mesoporous silica nanoparticles in recent photodynamic therapy applications

Author

Jean-Olivier Durand, Rabah Boukherroub, Sébastien Richeter, Laurence Raehm, Alexandre Barras, Sumeyra Bayir, Sabine Szunerits, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Department of Chemistry,Gebze Technical UniversityP. K.:141, 41400 Gebze, Kocaeli (Turkey), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM), and Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Materials science, Surface Properties, medicine.medical_treatment, Nanoparticle, Nanotechnology, Photodynamic therapy, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Alzheimer Disease, Neoplasms, medicine, [CHIM]Chemical Sciences, Physical and Theoretical Chemistry, Particle Size, Photosensitizing Agents, Bacteria, Mesoporous silica, 021001 nanoscience & nanotechnology, Atherosclerosis, Silicon Dioxide, 0104 chemical sciences, 3. Good health, Photochemotherapy, Nanoparticles, 0210 nano-technology, Porosity
Abstract

International audience; In this review, the use of mesoporous silica nanoparticles for photodynamic therapy (PDT) applications is described for the year 2017. Since the pioneering work in 2009, nanosystems involving mesoporous silica nanoparticles have gained in complexity with a sophisticated core–shell system able to perform multi-imaging and multi-therapies, not only for cancer diseases but also for anti-microbial therapy, atherosclerosis, or Alzheimer disease. Near-infrared, excitation light based on up-converting systems, X-rays or persistent luminescent systems are described for deeper tissue treatments.

Published
2018

17. Nanodiamond–PMO for two-photon PDT and drug delivery

Author

Rabah Boukherroub, Vanja Stojanovic, Yolanda Galàn Rubio, Ouahiba Hocine, Marcel Garcia, Dina Aggad, J.O. Durand, Nikola Knezevic, Chiara Mauriello Jimenez, Magali Gary-Bobo, Florina Teodorescu, Marie Maynadier, Laurence Raehm, Martina Seric, Sabine Szunerits, Jonas G. Croissant, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), NanoMedSyn, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM), and NanoMedSyn (NMS)

Subjects
Materials science, Biomedical Engineering, Nanoparticle, Nanotechnology, 02 engineering and technology, 010402 general chemistry, Photochemistry, 01 natural sciences, Two-photon excitation microscopy, medicine, General Materials Science, Doxorubicin, Nanodiamond, ComputingMilieux_MISCELLANEOUS, chemistry.chemical_classification, Reactive oxygen species, [CHIM.ORGA]Chemical Sciences/Organic chemistry, General Chemistry, General Medicine, 021001 nanoscience & nanotechnology, 0104 chemical sciences, 3. Good health, Mesoporous organosilica, chemistry, Drug delivery, Cancer cell, 0210 nano-technology, medicine.drug
Abstract

In this article, we highlight the properties of nanodiamonds (ND), which were encapsulated in periodic mesoporous organosilica nanoparticles (PMO) and were able to generate reactive oxygen species for photodynamic applications upon two-photon excitation (TPE). The ND@PMO nanoparticles were characterized by various techniques and were then loaded with the anti-cancer drug doxorubicin. The release of the drug was pH sensitive and a synergistic cancer cell killing effect was observed when cancer cells were incubated with doxorubicin-loaded ND@PMO and irradiated with two-photon excitation at 800 nm.

Published
2016

18. Hollow Organosilica Nanoparticles for Drug Delivery

Author

Christophe Nguyen, Saher Rahmani, Jelena Budimir, Alia Akrout, Morgane Daurat, Clarence Charnay, Magali Gary-Bobo, Dina Aggad, Laurence Raehm, Anastasia Godefroy, Hanene Largot, Jean-Olivier Durand, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), and Nanomedsyn

Subjects
Nanoparticle, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Gemcitabine Hydrochloride, medicine, [CHIM]Chemical Sciences, Microemulsion, skin and connective tissue diseases, Chemistry, digestive, oral, and skin physiology, technology, industry, and agriculture, General Chemistry, 021001 nanoscience & nanotechnology, Uniform size, Gemcitabine, 0104 chemical sciences, Methotrexate, MCF-7 breast cancer cells, Organosilica Nanoparticles, Drug delivery, Breast cancer cells, 0210 nano-technology, medicine.drug, Nuclear chemistry
Abstract

International audience; The sol‐gel synthesis of hollow organosilica nanoparticles incorporating amino groups with uniform size are described. These nanoparticles were successfully prepared via a microemulsion method. Then, the hollow nanoparticles were loaded with gemcitabine hydrochloride or methotrexate and studied in MCF‐7 breast cancer cells.

Published
2018

19. Chick chorioallantoic membrane assay as an in vivo model to study the effect of nanoparticle-based anticancer drugs in ovarian cancer

Author

Jonas G. Croissant, Altagracia Conteras, Laurence Raehm, Tammy Yik, Kotaro Matsumoto, Fuyuhiko Tamanoi, Niveen M. Khashab, Laura E. Ratliff, Tan Le Hoang Doan, Carlotta A. Glackin, Yevhen Fatieiev, Shirleen I. Simargi, Chiara Mauriello Jimenez, Sophia Allaf Shahin, Binh Thanh Vu, Jean-Olivier Durand, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier ( ICGM ICMMM ), Université Montpellier 1 ( UM1 ) -Université Montpellier 2 - Sciences et Techniques ( UM2 ) -Ecole Nationale Supérieure de Chimie de Montpellier ( ENSCM ) -Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ), Microbiology, Immunology and Molecular Genetics ( UCLA ), University of California at Los Angeles [Los Angeles] ( UCLA ), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Microbiology, Immunology and Molecular Genetics (UCLA), University of California [Los Angeles] (UCLA), and University of California-University of California

Subjects
0301 basic medicine, 02 engineering and technology, Chick Embryo, Chorioallantoic Membrane, Models, Nanotechnology, ComputingMilieux_MISCELLANEOUS, Cancer, Ovarian Neoplasms, Drug Carriers, Multidisciplinary, Tumor, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Chemistry, 021001 nanoscience & nanotechnology, Ovarian Cancer, 3. Good health, Chorioallantoic membrane, 5.1 Pharmaceuticals, Drug delivery, embryonic structures, Medicine, Biological Assay, Female, Development of treatments and therapeutic interventions, 0210 nano-technology, Biotechnology, medicine.drug, Stromal cell, Science, Bioengineering, Models, Biological, Article, Cell Line, 03 medical and health sciences, Targeted therapies, Rare Diseases, In vivo, [ CHIM.ORGA ] Chemical Sciences/Organic chemistry, Cell Line, Tumor, medicine, Animals, Humans, Doxorubicin, medicine.disease, Biological, Orphan Drug, 030104 developmental biology, Cell culture, Cancer research, Nanoparticles, Ovarian cancer
Abstract

New therapy development is critically needed for ovarian cancer. We used the chicken egg CAM assay to evaluate efficacy of anticancer drug delivery using recently developed biodegradable PMO (periodic mesoporous organosilica) nanoparticles. Human ovarian cancer cells were transplanted onto the CAM membrane of fertilized eggs, resulting in rapid tumor formation. The tumor closely resembles cancer patient tumor and contains extracellular matrix as well as stromal cells and extensive vasculature. PMO nanoparticles loaded with doxorubicin were injected intravenously into the chicken egg resulting in elimination of the tumor. No significant damage to various organs in the chicken embryo occurred. In contrast, injection of free doxorubicin caused widespread organ damage, even when less amount was administered. The lack of toxic effect of nanoparticle loaded doxorubicin was associated with specific delivery of doxorubicin to the tumor. Furthermore, we observed excellent tumor accumulation of the nanoparticles. Lastly, a tumor could be established in the egg using tumor samples from ovarian cancer patients and that our nanoparticles were effective in eliminating the tumor. These results point to the remarkable efficacy of our nanoparticle based drug delivery system and suggests the value of the chicken egg tumor model for testing novel therapies for ovarian cancer., がんの個別化医療を可能にする患者癌由来の鶏卵モデル. 京都大学プレスリリース. 2018-06-06.

Published
2018

20. Porous Porphyrin-Based Organosilica Nanoparticles for NIR Two-Photon Photodynamic Therapy and Gene Delivery in Zebrafish

Author

Nadir Bettache, Jonas G. Croissant, Vincent Chaleix, Michel Wong Chi Man, Mireille Rossel, Xavier Cattoën, Karen Tresfield, Niveen M. Khashab, Danielle Laurencin, Sébastien Richeter, Rachid Sougrat, Sébastien Clément, Jean-Olivier Durand, Chiara Mauriello Jimenez, Dina Aggad, Magali Gary-Bobo, Nicolas Cubedo, Erwan Oliviero, Marcel Garcia, Vincent Sol, Shahad Alsaiari, Marie Maynadier, Laurence Raehm, Manuel A. Roldan-Gutierrez, Clarence Charnay, Dorothée Berthomieu, Dalaver H. Anjum, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Mécanismes moléculaires dans les démences neurodégénératives (MMDN), Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), King Abdullah University of Science and Technology (KAUST), KAUST Catalysis Center (KCC), King Abdullah University of Sciences & Technologie, Optique et Matériaux (OPTIMA ), Institut Néel (NEEL), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), NanoMedSyn, Laboratoire de Chimie des Substances Naturelles (LCSN), Université de Limoges (UNILIM)-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503), Dynamique moléculaire des interactions membranaires (DMIM), and Centre National de la Recherche Scientifique (CNRS)-Université Montpellier 2 - Sciences et Techniques (UM2)

Subjects
Materials science, medicine.medical_treatment, Confocal, Nanoparticle, Photodynamic therapy, Nanotechnology, 02 engineering and technology, Gene delivery, 010402 general chemistry, 01 natural sciences, Biomaterials, chemistry.chemical_compound, Two-photon excitation microscopy, Electrochemistry, medicine, Zebrafish, Multiphoton imaging, ComputingMilieux_MISCELLANEOUS, biology, [CHIM.ORGA]Chemical Sciences/Organic chemistry, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, Condensed Matter Physics, biology.organism_classification, Porphyrin, 0104 chemical sciences, Electronic, Optical and Magnetic Materials, chemistry, 0210 nano-technology
Abstract

C.M.J. and D.A. contributed equally to this work. The grant “Chercheur d'Avenir Languedoc-Roussillon” attributed to M.G.-B. and the ANR nanoptPDT are gratefully acknowledged. The authors thank L. Lichon for technical assistance. DFT calculations were performed using HPC resources from the CINES (projects x2016087394 and x2015087394), and from the CALMIP (Grant 2016-[P16042]). The authors thank MRI (Montpellier RIO Imaging platform) for confocal and multiphoton imaging facilities.

Published
2018

21. Two-Photon-Excited Silica and Organosilica Nanoparticles for Spatiotemporal Cancer Treatment

Author

Laurence Raehm, Jean-Olivier Durand, Jeffrey I. Zink, Jonas G. Croissant, Chemical and Biological Engineering [Albuquerque], The University of New Mexico [Albuquerque], Center for Micro-Engineered Materials [Albuquerque] (CMEM), Department of Chemistry and Biochemistry, University of California Los Angeles, University of California [Los Angeles] (UCLA), University of California-University of California, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), and Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC)

Subjects
medicine.medical_treatment, Biomedical Engineering, Pharmaceutical Science, Nanoparticle, Nanotechnology, Photodynamic therapy, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Nanomaterials, Biomaterials, chemistry.chemical_compound, Drug Delivery Systems, Two-photon excitation microscopy, Neoplasms, medicine, [CHIM]Chemical Sciences, Humans, Chemistry, Singlet oxygen, Mesoporous silica, 021001 nanoscience & nanotechnology, Silicon Dioxide, 3. Good health, 0104 chemical sciences, Cancer treatment, Photochemotherapy, Drug delivery, Nanoparticles, 0210 nano-technology, Porosity
Abstract

International audience; Coherent two‐photon‐excited (TPE) therapy in the near‐infrared (NIR) provides safer cancer treatments than current therapies lacking spatial and temporal selectivities because it is characterized by a 3D spatial resolution of 1 µm3 and very low scattering. In this review, the principle of TPE and its significance in combination with organosilica nanoparticles (NPs) are introduced and then studies involving the design of pioneering TPE‐NIR organosilica nanomaterials are discussed for bioimaging, drug delivery, and photodynamic therapy. Organosilica nanoparticles and their rich and well‐established chemistry, tunable composition, porosity, size, and morphology provide ideal platforms for minimal side‐effect therapies via TPE‐NIR. Mesoporous silica and organosilica nanoparticles endowed with high surface areas can be functionalized to carry hydrophobic and biologically unstable two‐photon absorbers for drug delivery and diagnosis. Currently, most light‐actuated clinical therapeutic applications with NPs involve photodynamic therapy by singlet oxygen generation, but low photosensitizing efficiencies, tumor resistance, and lack of spatial resolution limit their applicability. On the contrary, higher photosensitizing yields, versatile therapies, and a unique spatial resolution are available with engineered two‐photon‐sensitive organosilica particles that selectively impact tumors while healthy tissues remain untouched. Patients suffering pathologies such as retinoblastoma, breast, and skin cancers will greatly benefit from TPE‐NIR ultrasensitive diagnosis and therapy.

Published
2017

22. Hybrid Particles

Author

Laurence Raehm, Jean-Olivier Durand, and Nikola Ž. Knežević

Subjects
chemistry.chemical_classification, chemistry, Biomolecule, Nanotechnology, 02 engineering and technology, 010402 general chemistry, 021001 nanoscience & nanotechnology, 0210 nano-technology, 01 natural sciences, 0104 chemical sciences, Nanomaterials
Published
2017

23. Biodegradable Ethylene-Bis(Propyl)Disulfide-Based Periodic Mesoporous Organosilica Nanorods and Nanospheres for Efficient In-Vitro Drug Delivery

Author

Jean-Olivier Durand, Michel Wong Chi Man, Jonas G. Croissant, Audrey Gallud, Philippe Trens, Marie Maynadier, Xavier Cattoën, Laurence Raehm, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), and Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)

Subjects
Nanostructure, Materials science, Ethylene, Cell Survival, Nanoparticle, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Ethylene bis, chemistry.chemical_compound, medicine, Humans, Organic chemistry, General Materials Science, Doxorubicin, Disulfides, Drug Carriers, Antibiotics, Antineoplastic, Nanotubes, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Mechanical Engineering, Ethylenes, Hydrogen-Ion Concentration, Silicon Dioxide, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Mesoporous organosilica, Chemical engineering, chemistry, Mechanics of Materials, Drug delivery, MCF-7 Cells, Nanorod, 0210 nano-technology, Porosity, Nanospheres, medicine.drug
Abstract

International audience; Periodic mesoporous organosilica nanorods and nanospheres are synthesized from 1,4-bis(triethoxysilyl)ethylene and bis(3-ethoxysilylpropyl)disulfide. The nanosystems present the long-range order of the hexagonal nanostructure. They are degraded in simulated physiological conditions. The loading and release of doxorubicin with these nanosystems are both pH dependent. These nanoparticles are endocytosed by breast cancer cells and are very efficient for doxorubicin delivery in these cells.

Published
2014

24. Front Cover: Organosilica Nanoparticles for Gemcitabine Monophosphate Delivery in Cancer Cells (ChemNanoMat 7/2019)

Author

Saher Rahmani, Clarence Charnay, Yannick Guari, Laurence Raehm, Morgane Daurat, Magali Gary-Bobo, Christophe Nguyen, Peter Hesemann, Roza Bouchal, Jelena Budimir, Alia Akrout, Nadir Bettache, Jean-Olivier Durand, Sébastien Richeter, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), and Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC)

Subjects
Renewable Energy, Sustainability and the Environment, Chemistry, Energy Engineering and Power Technology, Nanoparticle, Cancer, 02 engineering and technology, 010402 general chemistry, 021001 nanoscience & nanotechnology, medicine.disease, 01 natural sciences, Gemcitabine, 0104 chemical sciences, Biomaterials, Front cover, Cancer cell, Drug delivery, Materials Chemistry, Cancer research, medicine, [CHIM]Chemical Sciences, 0210 nano-technology, ComputingMilieux_MISCELLANEOUS, medicine.drug
Abstract

International audience

Published
2019

25. Mesoporous silicon nanoparticles for targeted two-photon theranostics of prostate cancer

Author

Vanja Stojanovic, Alain Morère, Nikola Knezevic, Frédérique Cunin, Elise Bouffard, Sébastien Richeter, Arnaud Chaix, Philippe Maillard, Khaled El Cheikh, Dina Aggad, Magali Gary-Bobo, Marcel Garcia, Jean-Olivier Durand, Marie Maynadier, Laurence Raehm, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Nanomedsyn, Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), NanoMedSyn, Distributed Programming Laboratory (LPD), Ecole Polytechnique Fédérale de Lausanne (EPFL), and Institut Curie, Research Center, PSL Research University

Subjects
Materials science, Silicon, medicine.medical_treatment, [SDV]Life Sciences [q-bio], Biomedical Engineering, Nanoparticle, chemistry.chemical_element, Photodynamic therapy, Nanotechnology, 02 engineering and technology, [CHIM.INOR]Chemical Sciences/Inorganic chemistry, 010402 general chemistry, Porous silicon, 01 natural sciences, Prostate cancer, Two-photon excitation microscopy, medicine, [CHIM]Chemical Sciences, General Materials Science, technology, industry, and agriculture, General Chemistry, General Medicine, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, medicine.disease, equipment and supplies, 0104 chemical sciences, chemistry, 0210 nano-technology, Mesoporous material
Abstract

International audience; A novel non-toxic porous silicon nanoparticle grafted with a mannose-6-phosphate analogue and applicable in 2-photon imaging and photodynamic therapy was specifically designed for targeting prostate cancer cells.

Published
2016

26. Multifunctional Gold-Mesoporous Silica Nanocomposites for Enhanced Two-Photon Imaging and Therapy of Cancer Cells

Author

Jonas G. Croissant, Mireille Blanchard-Desce, Christian Qi, Michel Wong Chi Man, Marcel Garcia, Magali Gary-Bobo, Olivier Mongin, Jean-Olivier Durand, Xavier Cattoën, Marie Maynadier, Laurence Raehm, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), NanoMedSyn (NMS), Optique et Matériaux (NEEL - OPTIMA), Institut Néel (NEEL), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1 (UB)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), NanoMedSyn, Optique et Matériaux (OPTIMA ), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), and Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Université Sciences et Technologies - Bordeaux 1-Université Montesquieu - Bordeaux 4-Institut de Chimie du CNRS (INC)

Subjects
Materials science, Nanoparticle, Nanotechnology, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Biochemistry, Genetics and Molecular Biology (miscellaneous), Biochemistry, mesoporous silica nanoparticles, lcsh:QH301-705.5, Molecular Biology, Original Research, two-photon, Nanocomposite, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Bioengineering and Biotechnology, Mesoporous silica, Mesoporous Silica Nanoparticles, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Mesoporous organosilica, lcsh:Biology (General), organic-inorganic, photodynamic therapy, Colloidal gold, gold nanoparticles, Nanomedicine, Nanocarriers, 0210 nano-technology, Mesoporous material
Abstract

International audience; Three dimensional sub-micron resolution has made two-photon nanomedicine a very promising medical tool for cancer treatment since current techniques cause significant side effects for lack of spatial selectivity. Two-photon-excited (TPE) photodynamic therapy (PDT) has been achieved via mesoporous nanoscaffolds, but the efficiency of the treatment could still be improved. Herein, we demonstrate the enhancement of the treatment efficiency via gold-mesoporous organosilica nanocomposites for TPE-PDT in cancer cells when compared to mesoporous organosilica particles. We performed the first comparative study of the influence of the shape and spatial position of gold nanoparticles (AuNPs) with mesoporous silica nanoparticles (MSN) functionalized with thiol groups and doped with a two-photon electron donor (2PS). The resulting multifunctional nanocarriers displayed TPE-fluorescence and were imaged inside cells. Furthermore, mesoporous organosilica NPs decorated gold nanospheres (AuNSs) induced 63 percent of selective killing on MCF-7 breast cancer cells. This study thus provides insights for the design of more effective multifunctional two-photon-sensitive nanocomposites via AuNPs for biomedical applications.

Published
2016

27. 20-nm-sized mesoporous silica nanoparticles with porphyrin photosensitizers for in vitro photodynamic therapy

Author

Vanja Stojanovic, Alain Morère, Magali Gary-Bobo, Philippe Maillard, Philippe Arnoux, Valentin Saunier, Chiara Mauriello Jimenez, David Warther, Nadir Bettache, Marie Maynadier, Laurence Raehm, Marcel Garcia, Jean-Olivier Durand, Céline Frochot, Yolanda Galàn Rubio, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Laboratoire Réactions et Génie des Procédés (LRGP), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), and Institut Curie [Paris]

Subjects
Materials science, medicine.medical_treatment, Nanoparticle, Photodynamic therapy, 02 engineering and technology, Mesoporous, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, 010402 general chemistry, 01 natural sciences, Biomaterials, Porphyrin, chemistry.chemical_compound, Pulmonary surfactant, PDT, Bromide, Materials Chemistry, medicine, General Chemistry, [CHIM.MATE]Chemical Sciences/Material chemistry, Mesoporous silica, 021001 nanoscience & nanotechnology, Condensed Matter Physics, Silica nanoparticles, 0104 chemical sciences, Electronic, Optical and Magnetic Materials, chemistry, silica, Ceramics and Composites, Surface modification, functionalization, nanoparticles, 0210 nano-technology, Mesoporous material, Nuclear chemistry
Abstract

International audience; We report the synthesis of 20-nm-sized mesoporous silica nanoparticles encapsulating three different porphyrin photosensitizers (PS). Synthesized by the sol–gel method, in the presence of cetyltrimethylammonium bromide surfactant and tetraorthosilicate as silica source, we developed those nanoparticles with the aim of treating cancer cells using photodynamic therapy. The colloidal stability, due to the PEG-silane chain grafted on the surface,was demonstrated at 37 C in cell culture medium. Then, nanoparticles were functionalized by silylated squarate mannose and used in MCF-7 breast cancer cells for one-photon therapy. Promising results were obtained after 5 h of incubation.

Published
2016

28. Functionalisation of mesoporous silica nanoparticles with 3-isocynatopropyltrichlorosilane

Author

Wassim El Malti, Laurence Raehm, Jean-Olivier Durand, Mireille Blanchard-Desce, Olivier Mongin, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Chimie et Photonique Moléculaires (CPM), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS)

Subjects
animal structures, [CHIM.ORGA]Chemical Sciences/Organic chemistry, General Chemical Engineering, Nanoparticle, 02 engineering and technology, General Chemistry, Mesoporous silica, 010402 general chemistry, 021001 nanoscience & nanotechnology, Grafting, 01 natural sciences, Isocyanate, 0104 chemical sciences, chemistry.chemical_compound, chemistry, Nucleophile, Covalent bond, Polymer chemistry, Urea, Organic chemistry, Reactivity (chemistry), sense organs, 0210 nano-technology, ComputingMilieux_MISCELLANEOUS
Abstract

The functionalisation of Mesoporous Silica Nanoparticles (MSN) with the isocyanate group was carried out. The excellent reactivity of 3-isocynanatopropyltrichlorosilane allowed its grafting on the surface of MSN in mild conditions. Further reaction with different nucleophiles bearing primary amino groups led to the formation of a urea linkage and thus the covalent grafting of the nucleophiles to the MSN surface.

Published
2011

29. Photodynamic Therapy: Porous Porphyrin-Based Organosilica Nanoparticles for NIR Two-Photon Photodynamic Therapy and Gene Delivery in Zebrafish (Adv. Funct. Mater. 21/2018)

Author

Marcel Garcia, Nicolas Cubedo, Rachid Sougrat, Vincent Sol, Jean-Olivier Durand, Erwan Oliviero, Magali Gary-Bobo, Vincent Chaleix, Danielle Laurencin, Mireille Rossel, Karen Tresfield, Niveen M. Khashab, Xavier Cattoën, Nadir Bettache, Dorothée Berthomieu, Sébastien Clément, Chiara Mauriello Jimenez, Manuel A. Roldan-Gutierrez, Sébastien Richeter, Dina Aggad, Jonas G. Croissant, Michel Wong Chi Man, Marie Maynadier, Laurence Raehm, Dalaver H. Anjum, Shahad Alsaiari, and Clarence Charnay

Subjects
Materials science, biology, medicine.medical_treatment, Nanoparticle, Photodynamic therapy, 02 engineering and technology, Gene delivery, 010402 general chemistry, 021001 nanoscience & nanotechnology, Condensed Matter Physics, biology.organism_classification, 01 natural sciences, Porphyrin, 0104 chemical sciences, Electronic, Optical and Magnetic Materials, Biomaterials, chemistry.chemical_compound, Two-photon excitation microscopy, chemistry, Electrochemistry, Zebrafish embryo, medicine, Biophysics, 0210 nano-technology, Zebrafish
Published
2018

30. Surface Modifications of Love Acoustic Waves Sensors for Chemical and Biological Detection

Author

Rémi Desmet, Marc Rolland, Corinne Dejous, Dominique Rebière, Oleg Melnyk, Jean Olivier Durand, Marc Cretin, Michel Persin, Laurent Fertier, Laurence Raehm, Institut Européen des membranes (IEM), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM), Laboratoire de l'intégration, du matériau au système (IMS), Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Institut de biologie de Lille - IBL (IBLI), Université de Lille, Sciences et Technologies-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Centre National de la Recherche Scientifique (CNRS)-Université de Lille, Droit et Santé, Institut de Biologie, Centre National de la Recherche Scientifique (CNRS), and UMR 8525

Subjects
Materials science, Acoustics, 02 engineering and technology, 01 natural sciences, Surface Functionalization, chemistry.chemical_compound, SELF-ASSEMBLED MONOLAYERS, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, BINDING, 0103 physical sciences, Molecule, Chelation, Electrical and Electronic Engineering, SILICON, ComputingMilieux_MISCELLANEOUS, SH-SAW, AGENT, Thin Films, 010302 applied physics, Semicarbazide, [CHIM.ORGA]Chemical Sciences/Organic chemistry, PEPTIDES, TRANSDUCERS, 021001 nanoscience & nanotechnology, Combinatorial chemistry, Atomic and Molecular Physics, and Optics, ARRAYS, Transducer, chemistry, Thiourea, Covalent bond, Chemisorption, DENSITY, FUNCTIONALIZATION, Surface modification, METAL IONS, 0210 nano-technology
Abstract

The surface functionalization of guided-SH-SAW (Shear Horizontal-Surface Acoustic Wave) transducers was carried out to prepare layers for heavy metals chelation and antibodies recognition. For the preparation of the chemical sensors, a thiourea molecule acting as a chelating agent for heavy metals was grafted on the top-layer of the transducer. This covalent immobilization was obtained by amide bonds through primary amine groups immobilized on the device by the chemisorption of the 3-aminopropyltriethoxysilane (APTES). The thiourea modified transducer was inserted in a microfluidic analysis system to test its sensitivity for cadmium ions in liquid media. In the aim to develop bio-chemical sensors based on antigen/antibody recognition, a functionalization way based on SAMs of semicarbazide functions leading to alpha-oxo-semicarbazone bonds with antigenic peptides is described. The real-time immobilization of the peptide on the surface was observed for the first time and the antibody recognition was successfully monitored. Based on these results, the gravimetric determination by guided-SH-SAW transducers functionalized by the semicarbazide/alpha-oxo-aldehyde chemistry is a promising complementary route for antibodies analysis.

Published
2009

31. Love wave immunosensor for antibody recognition using an innovative semicarbazide surface functionalization

Author

Corinne Dejous, Oleg Melnyk, Jean-Olivier Durand, Laurent Fertier, Céline Zimmermann, Marc Cretin, Rémi Desmet, Laurence Raehm, Dominique Rebière, Marc Rolland, Institut Européen des membranes (IEM), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Institut de biologie de Lille - IBL (IBLI), Université de Lille, Sciences et Technologies-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Centre National de la Recherche Scientifique (CNRS)-Université de Lille, Droit et Santé, Laboratoire de l'intégration, du matériau au système (IMS), and Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS)

Subjects
medicine.drug_class, Nanotechnology, 02 engineering and technology, 010402 general chemistry, Monoclonal antibody, 01 natural sciences, chemistry.chemical_compound, Materials Chemistry, Fluorescence microscope, medicine, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, Electrical and Electronic Engineering, Instrumentation, ComputingMilieux_MISCELLANEOUS, Semicarbazide, biology, Atomic force microscopy, Metals and Alloys, Acoustic sensor, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, Condensed Matter Physics, 0104 chemical sciences, 3. Good health, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Love wave, chemistry, biology.protein, Surface modification, Antibody, 0210 nano-technology
Abstract

A new novel and easy functionalization route of a Love wave acoustic sensor based on the α-oxo-semicarbazone bond is described. The interest is firstly to observe in real-time the immobilization of the peptide on the semicarbazide surface of the transducer and secondly to monitor the specific binding of antibodies. Site-specific immobilization of antigenic-peptides as well as binding of murine monoclonal antibodies has been shown by gravimetric measurements. A tetramethylrhodamine-labeled goat antibody directed against murine antibodies was used to further characterize the biomolecular interactions by fluorescence microscopy and surface analysis (by AFM). Our data show that the gravimetric monitoring developed from the prepared Love wave immunosensor is a promising alternative route to characterize chemical and biomolecular events.

Published
2009

32. Porphyrin-functionalized mesoporous organosilica nanoparticles for two-photon imaging of cancer cells and drug delivery

Author

Chiara Mauriello-Jimenez, Michel Wong Chi Man, Vincent Sol, Marcel Garcia, Julien Vergnaud, Jonas G. Croissant, Xavier Cattoën, Vincent Chaleix, Magali Gary-Bobo, Marie Maynadier, Laurence Raehm, Jean-Olivier Durand, Médicaments Photoactivables - Photochimiothérapie (PHOTOMED), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), NanoMedSyn, Optique et Matériaux (OPTIMA), Institut Néel (NEEL), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie des Substances Naturelles (LCSN), and Université de Limoges (UNILIM)-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503)

Subjects
Materials science, Biomedical Engineering, Nanoparticle, Nanotechnology, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, chemistry.chemical_compound, polycyclic compounds, medicine, General Materials Science, Doxorubicin, ComputingMilieux_MISCELLANEOUS, [CHIM.ORGA]Chemical Sciences/Organic chemistry, General Chemistry, General Medicine, 021001 nanoscience & nanotechnology, Porphyrin, Combinatorial chemistry, 0104 chemical sciences, Mesoporous organosilica, chemistry, Covalent bond, Drug delivery, Cancer cell, 0210 nano-technology, Derivative (chemistry), medicine.drug
Abstract

The synthesis of a zinc porphyrin derivative possessing eight triethoxysilyl groups was performed through a CuAAC-click reaction. This porphyrin was covalently entrapped in ethenylene-bridged mesoporous organosilica nanoparticles which efficiently allowed performing doxorubicin delivery and two-photon imaging of breast cancer cells.

Published
2015

33. Mixed Periodic Mesoporous Organosilica Nanoparticles and Core−Shell Systems, Application to in Vitro Two-Photon Imaging, Therapy, and Drug Delivery

Author

Audrey Gallud, Laurence Raehm, Xavier Cattoën, Mireille Blanchard-Desce, Jonas G. Croissant, Michel Wong Chi Man, Marcel Garcia, Jean-Olivier Durand, Vincent Hugues, Marie Maynadier, Damien Salles, Magali Gary-Bobo, Olivier Mongin, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Institut des Sciences Chimiques de Rennes (ISCR), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Institut des Sciences Moléculaires (ISM), Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Université Sciences et Technologies - Bordeaux 1-Université Montesquieu - Bordeaux 4-Institut de Chimie du CNRS (INC), Optique et Matériaux (OPTIMA), Institut Néel (NEEL), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), ANR P2N 2010, ANR-10-NANO-0022,MECHANANO,Nanomachines mécanisées pour l'activation à deux photons(2010), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1 (UB)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Optique et Matériaux (NEEL - OPTIMA), and Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)

Subjects
Materials science, General Chemical Engineering, Nanoparticle, Nanotechnology, 02 engineering and technology, General Chemistry, [CHIM.MATE]Chemical Sciences/Material chemistry, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, In vitro, 3. Good health, 0104 chemical sciences, Mesoporous organosilica, Two-photon excitation microscopy, Phenylene, Drug delivery, Materials Chemistry, Nanomedicine, [CHIM]Chemical Sciences, Photosensitizer, 0210 nano-technology
Abstract

International audience; In this work, we describe the synthesis of new Mixed Periodic Mesoporous Organosilica Nanoparticles (MPMO NPs), combining the co-condensation of a tetra-trialkoxysilylated twophoton photosensitizer with bis-(triethoxysilyl)phenylene or ethylene. Novel gold core-MPMO shell systems are also described. The MPMO NPs are analyzed and characterized by multiple techniques, and are very efficient for anti-cancer drug delivery combined with two-photon therapy in MCF-7 breast cancer cells, leading down to 76% cancer cell death. MPMO NPs are thus very promising for nanomedicine applications.

Published
2014

34. Functionalized mesoporous silica nanoparticles for one and two-photon photodynamic therapy

Author

Marcel Garcia, Alain Morère, Magali Gary-Bobo, J.O. Durand, Mireille Blanchard-Desce, Laurence Raehm, and Ph. Maillard

Subjects
Materials science, medicine.medical_treatment, Biophysics, Nanoparticle, Nanotechnology, Photodynamic therapy, 02 engineering and technology, Dermatology, Mesoporous silica, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, 0104 chemical sciences, Oncology, Two-photon excitation microscopy, medicine, Pharmacology (medical), 0210 nano-technology
Published
2017

35. Click approaches in sol-gel chemistry

Author

Kristýna Bürglová, Jonas G. Croissant, Jana Hodačová, Sylvie Begin-Colin, Laurence Raehm, J.O. Durand, Olivia de los Cobos, Xavier Cattoën, Achraf Noureddine, Benoit P. Pichon, Michel Wong Chi Man, Fabrice Rossignol, Martine Lejeune, Nirmalya Moitra, Delphine Toulemon, Optique et Matériaux (OPTIMA), Institut Néel (NEEL), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Institute of Chemical Technology [Prague] (ICT), Axe 1 : procédés céramiques, Science des Procédés Céramiques et de Traitements de Surface (SPCTS), Université de Limoges (UNILIM)-Ecole Nationale Supérieure de Céramique Industrielle (ENSCI)-Institut des Procédés Appliqués aux Matériaux (IPAM), Université de Limoges (UNILIM)-Université de Limoges (UNILIM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Limoges (UNILIM)-Ecole Nationale Supérieure de Céramique Industrielle (ENSCI)-Institut des Procédés Appliqués aux Matériaux (IPAM), Université de Limoges (UNILIM)-Université de Limoges (UNILIM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut de Physique et Chimie des Matériaux de Strasbourg (IPCMS), Université de Strasbourg (UNISTRA)-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, and Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)

Subjects
Materials science, Nanoparticle, Nanotechnology, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Organosilanes, Biomaterials, Monolayer, Materials Chemistry, Sol-gel, Click chemistry, General Chemistry, [CHIM.MATE]Chemical Sciences/Material chemistry, Mesoporous silica, 021001 nanoscience & nanotechnology, Condensed Matter Physics, Cycloaddition, 0104 chemical sciences, Electronic, Optical and Magnetic Materials, Ceramics and Composites, Surface modification, Magnetic nanoparticles, Nanoparticles, 0210 nano-technology, CuAAC
Abstract

International audience; The combination of the copper-catalyzed alkyneazide cycloaddition (CuAAC) reactionwith sol-gel processing enables the versatile preparation of sol-gel materials under different shapes with targeted functionalities through a diversity-oriented approach. In this account, the development of the CuAAC reaction under anhydrous conditions for the synthesis of sol-gel precursors and for the assembling of magnetic nanoparticles on self-assembled monolayers is related, as well as the use of the classical CuAAC methodologies for the functionalization of mesoporous silica nanoparticles and microdots arrays. Coupling CuAAC and Sol-Gel will result in simplified preparations of multifunctional materials with controlled morphologies.

Published
2014

36. Small sized mesoporous silica nanoparticles functionalized with mannose for retinoblastoma cell imaging

Author

Jean-Olivier Durand, Alain Morère, Audrey Gallud, Marcel Garcia, Marie Maynadier, Laurence Raehm, David Warther, Corine Gérardin, Khaled El Cheikh, Chiara Mauriello Jimenez, Magali Gary-Bobo, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), and Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)

Subjects
General Chemical Engineering, Cell, Nanoparticle, Mannose, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Suspension (chemistry), chemistry.chemical_compound, medicine, Organic chemistry, ComputingMilieux_MISCELLANEOUS, Retinoblastoma, [CHIM.ORGA]Chemical Sciences/Organic chemistry, General Chemistry, Mesoporous silica, 021001 nanoscience & nanotechnology, medicine.disease, Silane, 0104 chemical sciences, 3. Good health, medicine.anatomical_structure, chemistry, Chemical engineering, Surface modification, 0210 nano-technology
Abstract

We report the study of the functionalization of small sized MSNs with mannose. Classical procedures which were efficient with 100 nm diameter MSNs led to aggregation of small sized MSNs. Therefore, we grafted long chain amino silane and PEG-silane on the surface of the MSNs and reacted squarate mannose with NH2 groups. Although the MSN suspension was stable in EtOH, immediate aggregation was noticed in PBS which is attributed to remaining amino groups on the surface of the MSNs. We therefore grafted silylated mannose and PEG-silane on the MSN surface. Depending on the conditions, reversible aggregation was observed in PBS when heating to 37 °C and cooling to RT, or stable suspensions were obtained. The stable mannose-functionalized MSN suspensions were used for the imaging of retinoblastoma cells.

Published
2014

37. Versatile heavy metals removal via magnetic mesoporous nanocontainers

Author

Jérôme Long, Jonas G. Croissant, Laurence Raehm, Makhlouf Boufatit, Soroya Dib, J.O. Durand, Siham Chelouaou, F. Sadi-Hassaine, Clarence Charnay, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), and Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC)

Subjects
Materials science, [CHIM.ORGA]Chemical Sciences/Organic chemistry, General Chemical Engineering, Metal ions in aqueous solution, Inorganic chemistry, Iron oxide, Magnetic separation, 02 engineering and technology, General Chemistry, Mesoporous silica, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, 0104 chemical sciences, chemistry.chemical_compound, Adsorption, chemistry, Surface modification, 0210 nano-technology, Porosity, Mesoporous material, ComputingMilieux_MISCELLANEOUS
Abstract

Versatile heavy metal ions removal is performed via iron oxide core mesoporous silica shell nanocontainers functionalized with diethylene triamine pentaacetic acid. Magnetic separation from aqueous media is obtained for a dozen of the most toxic heavy metal ions with remarkable efficiencies. Furthermore, this study demonstrates enhancement of the adsorption capacities with the combination of porous and surface functionalization of the nanocontainers.

Published
2014

38. Two-Photon-Triggered Drug Delivery in Cancer Cells Using Nanoimpellers

Author

Jean-Olivier Durand, Marie Maynadier, Laurence Raehm, Jeffrey I. Zink, Jeff L. Nyalosaso, Mireille Blanchard-Desce, Magali Gary-Bobo, Marcel Garcia, Audrey Gallud, Olivier Mongin, Jie Lu, Harmel Peindy N'Dongo, Nathalie Cheminet, Derrick Tarn, Clarence Charnay, Françoise Serein-Spirau, Thibaut Jarrosson, Tania M. Guardado-Alvarez, Jonas G. Croissant, Gaelle Derrien, Fuyuhiko Tamanoi, Chemical and Biological Engineering [Albuquerque], The University of New Mexico [Albuquerque], Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie de Clermont-Ferrand - Clermont Auvergne (ICCF), Sigma CLERMONT (Sigma CLERMONT)-Université Clermont Auvergne (UCA)-Centre National de la Recherche Scientifique (CNRS), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Synthèse et électrosynthèse organiques (SESO), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), Institut de recherche en cancérologie de Montpellier (IRCM - U896 Inserm - UM1), CRLCC Val d'Aurelle - Paul Lamarque-Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 1 (UM1), Department of Chemistry, Massachusetts Institute of Technology (MIT), Microbiology, Immunology and Molecular Genetics (UCLA), University of California [Los Angeles] (UCLA), University of California-University of California, Department of Chemistry and Biochemistry, Exotic Materials Institute, University of California, Los Angeles (UCLA), Department of Chemistry and Biochemistry, University of California Los Angeles, Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Université Montpellier 1 (UM1)-CRLCC Val d'Aurelle - Paul Lamarque-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), University of California (UC)-University of California (UC), ANR-10-NANO-0022,MECHANANO,Nanomachines mécanisées pour l'activation à deux photons(2010), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Institut des Sciences Chimiques de Rennes (ISCR), Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Rennes-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES), Chimie et Photonique Moléculaires (CPM), Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Centre National de la Recherche Scientifique (CNRS), Nyalosaso, Jeff, and NANOTECHNOLOGIES ET NANOSYSTEMES - Nanomachines mécanisées pour l'activation à deux photons - - MECHANANO2010 - ANR-10-NANO-0022 - P2N - VALID

Subjects
Fluorophore, Stereochemistry, [CHIM.THER] Chemical Sciences/Medicinal Chemistry, [SDV.CAN]Life Sciences [q-bio]/Cancer, 02 engineering and technology, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, mesoporous materials, 010402 general chemistry, 01 natural sciences, Catalysis, Article, chemistry.chemical_compound, Drug Delivery Systems, Two-photon excitation microscopy, [SDV.CAN] Life Sciences [q-bio]/Cancer, Neoplasms, medicine, cancer, Humans, ComputingMilieux_MISCELLANEOUS, [CHIM.MATE] Chemical Sciences/Material chemistry, two-photon excitation, Chemistry, 010405 organic chemistry, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Cancer, General Chemistry, General Medicine, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, medicine.disease, [CHIM.ORGA] Chemical Sciences/Organic chemistry, 0104 chemical sciences, 3. Good health, Förster resonance energy transfer, azobenzene, Azobenzene, Drug delivery, Cancer cell, drug delivery, Biophysics, Nanoparticles, 0210 nano-technology, Azo Compounds, Camptothecin, medicine.drug
Abstract

International audience; A therapy of cancer cells: Two-photon-triggered camptothecin delivery (see picture) with nanoimpellers was studied in MCF-7 breast cancer cells. A fluorophore with a high two-photon absorption cross-section was first incorporated in the nanoimpellers. Fluorescence resonance energy transfer (FRET) from the fluorophore to the azobenzene moiety was demonstrated.

Published
2013

39. Nanoparticules de silice mésoporeuse optimisées pour la fluorescence à deux photons

Author

Laurence Raehm, Mireille Blanchard-Desce, Jean-Olivier Durand, Nicolas Nerambourg, Monique Smaïhi, Catherine Dubernet, Valérie Lebret, Martinus H. V. Werts, Corine Gérardin, and Delphine Méthy-Gonnod

Subjects
02 engineering and technology, General Medicine, 010402 general chemistry, 021001 nanoscience & nanotechnology, 0210 nano-technology, 01 natural sciences, General Biochemistry, Genetics and Molecular Biology, 0104 chemical sciences
Abstract

Les nanoparticules mesoporeuses ont des proprietes uniques : une grande surface specifique ou une distribution etroite des tailles de pores. Les perspectives d’utilisation sont la creation de nouveaux outils pour le diagnostic precoce. Pour ces potentielles applications biologiques, l’innocuite de ces nanoparticules doit etre etablie.

Published
2009

40. Mannose-functionalized porous silica-coated magnetic nanoparticles for two-photon imaging or PDT of cancer cells

Author

Marine Perrier, Philippe Maillard, Jean-Olivier Durand, Marcel Garcia, Yannick Guari, Mireille Blanchard-Desce, Magali Gary-Bobo, Olivier Mongin, Laurence Raehm, Joulia Larionova, Lenaic Lartigue, Alain Morère, David Brevet, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Conception, synthèse et vectorisation de biomolécules. (CSVB), Université Paris Descartes - Paris 5 (UPD5)-Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1 (UB)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Université Paris Descartes - Paris 5 (UPD5)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris], Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut Curie-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes-Centre National de la Recherche Scientifique (CNRS), and Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Fluorophore, Materials science, Mannose, Nanoparticle, Bioengineering, Nanotechnology, 02 engineering and technology, 010402 general chemistry, Photochemistry, 01 natural sciences, Photodynamic therapy, chemistry.chemical_compound, Moiety, General Materials Science, Photosensitizer, Two-photon imaging, [CHIM.ORGA]Chemical Sciences/Organic chemistry, General Chemistry, Mesoporous silica, 021001 nanoscience & nanotechnology, Condensed Matter Physics, Porphyrin, Atomic and Molecular Physics, and Optics, 0104 chemical sciences, chemistry, Modeling and Simulation, Magnetic nanoparticles, 0210 nano-technology
Abstract

International audience; An original fluorophore engineered for two-photon excitation or a porphyrin derivative were entrapped in the silica shell of magnetic porous silica nanoparticles during the synthesis of the silica moiety without damaging the structure of the organic part. The mild conditions involved allowed obtaining microporous or mesoporous silica magnetic nanoparticles, respectively. Mannose was grafted on the surface of the nanoparticles to target MCF-7 breast cancer cells. The studies of magnetic properties of these hybrid nanoparticles showthat they present a blocking temperature at 190 K. The nano-objects designed with the twophoton fluorophore were efficient for two-photon imaging of MCF-7 cancer cells, whereas the nanoobjects with the photosensitizer efficiently killed cancer cells. The presence of the mannose moiety was demonstrated to improve both imaging and therapy properties.

Published
2013

41. Functionalized Nanoparticles for Drug Delivery, One- and Two-photon Photodynamic Therapy as a Promising Treatment of Retinoblastoma

Author

Mireille Blanchard-Desce, Marie Maynadier, Laurence Raehm, Ilaria Basile, Afitz Da Silva, Marcel Garcia, Simon Fontanel, Cyndie Lemaire, Audrey Gallud, Magali Gary-Bobo, Olivier Mongin, Philippe Maillard, Alain Morère, Jean-Olivier Dur, Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Conception, synthèse et vectorisation de biomolécules. (CSVB), Institut Curie-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5), Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes-Centre National de la Recherche Scientifique (CNRS), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Université Paris Descartes - Paris 5 (UPD5)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris], Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Université Sciences et Technologies - Bordeaux 1-Université Montesquieu - Bordeaux 4-Institut de Chimie du CNRS (INC), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Université Paris Descartes - Paris 5 (UPD5)-Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1 (UB)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Drug, medicine.medical_treatment, media_common.quotation_subject, Photodynamic therapy, 02 engineering and technology, Pharmacology, 03 medical and health sciences, 0302 clinical medicine, Medicine, ComputingMilieux_MISCELLANEOUS, media_common, Chemotherapy, business.industry, Retinoblastoma, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Photosensitizing Agent, 021001 nanoscience & nanotechnology, medicine.disease, eye diseases, 3. Good health, Drug delivery, Cancer cell, 030221 ophthalmology & optometry, Cancer research, 0210 nano-technology, business, Camptothecin, medicine.drug
Abstract

Retinoblastoma is a rare cancer triggered by genetic mutation that forms in the eyes of children. In industrialized countries, 95% of patients are cured by chemotherapy and conservative treatments. However these treatments can increase the risk of secondary tumors in patients with a constitutional alteration of the retinoblastoma gene Rb1. Photodynamic therapy (PDT) represents a therapeutic approach and may reduce the incidence of secondary tumors. PDT is an established cancer treatment based on the light activation of a photosensitizing agent thus generating cytotoxic reactive oxygen species that cause cellular damage. We focused on mesoporous silica nanoparticles (MSN) for one-photon excited PDT combined with drug delivery and carbohydrate targeting applied on retinoblastoma. We demonstrated that bitherapy (camptothecin delivery and PDT) performed with MSN was efficient in inducing retinoblastoma cell death. Alternatively, MSN designed for two-photon excited PDT were also studied and irradiation in near-infrared at low fluency efficiently killed retinoblastoma cancer cells. These data provide new evidences of the potential of functionalized and targeted MSN for treatment of retinoblastoma and could lead to propose a non-invasive therapy with reduced side effects.

Published
2013

42. Non polymeric nanoparticles for photodynamic therapy applications: recent developments

Author

Régis Vanderesse, Jean-Olivier Durand, Hamanou Benachour, Céline Frochot, Marc Verelst, Sébastien Richeter, Muriel Barberi-Heyob, Aymeric Sève, Rima Chouikrat, Laurence Raehm, Laboratoire Réactions et Génie des Procédés (LRGP), Centre National de la Recherche Scientifique (CNRS)-Université de Lorraine (UL), Centre d'élaboration de matériaux et d'études structurales (CEMES), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie de Toulouse (ICT-FR 2599), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut de Chimie du CNRS (INC)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA), Laboratoire de Chimie Physique Macromoléculaire (LCPM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Lorraine (UL), Centre de Recherche en Automatique de Nancy (CRAN), Université Henri Poincaré - Nancy 1 (UHP)-Institut National Polytechnique de Lorraine (INPL)-Centre National de la Recherche Scientifique (CNRS), Centre Alexis Vautrin (CAV), Médicaments Photoactivables - Photochimiothérapie (PHOTOMED), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut de Chimie de Toulouse (ICT), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), and Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
photosensitizer, medicine.medical_treatment, Nanotechnology, Photodynamic therapy, 02 engineering and technology, 010402 general chemistry, chemotherapy, 01 natural sciences, Biochemistry, Magnetics, Nanoparticle, Drug Discovery, medicine, Animals, Humans, cancer, Photosensitizer, radiotherapy, Pharmacology, Titanium, reactive oxygen species, Photosensitizing Agents, Chemistry, Nanotubes, Carbon, nanoplatforms, Organic Chemistry, Cancer, 021001 nanoscience & nanotechnology, Polymeric nanoparticles, medicine.disease, Silicon Dioxide, nanomedicine, polymeric, 3. Good health, 0104 chemical sciences, Cancer treatment, Radiation therapy, Photochemotherapy, photodynamic therapy, Molecular Medicine, Nanomedicine, Nanoparticles, [SDV.IB]Life Sciences [q-bio]/Bioengineering, Gold, Zinc Oxide, 0210 nano-technology
Abstract

International audience; Photodynamic therapy has emerged as an alternative to chemotherapy and radiotherapy for cancer treatment. Nanoparticles have recently been proposed as effective carriers for photosensitizers. Depending on their chemical composition, these can be used for diagnosis and therapy due to the selective accumulation of the photosensitizer in cancer cells in vitro or in tumors in vivo. Multifunctional nanoplatforms combining several applications within the same nano-object emerge as potential important theranostic tools. This review, based on the chemical nature of the nanoparticles will discuss recent advances in the area of non polymeric nanoparticles for photodynamic therapy applications.

Published
2012

43. Cancer Therapy Improvement with Mesoporous Silica Nanoparticles Combining Targeting, Drug Delivery and PDT

Author

Magali Gary-Bobo, Virginie Charasson, Jean-Olivier Durand, Philippe Maillard, David Brevet, Sébastien Richeter, Marcel Garcia, Bernard Loock, Ouahiba Hocine, Alain Morère, Marie Maynadier, Laurence Raehm, Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Conception, synthèse et vectorisation de biomolécules. (CSVB), Université Paris Descartes - Paris 5 (UPD5)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris], Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), and Institut Curie-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)

Subjects
animal structures, Time Factors, medicine.medical_treatment, Chemistry, Pharmaceutical, Drug Compounding, Pharmaceutical Science, Photodynamic therapy, Receptors, Cell Surface, 02 engineering and technology, Endosomes, 010402 general chemistry, 01 natural sciences, law.invention, Confocal microscopy, law, Neoplasms, medicine, Humans, Nanotechnology, Technology, Pharmaceutical, Photosensitizer, ComputingMilieux_MISCELLANEOUS, Drug Carriers, Microscopy, Confocal, Photosensitizing Agents, Cell Death, Chemistry, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Galactose, Mesoporous silica, 021001 nanoscience & nanotechnology, HCT116 Cells, Silicon Dioxide, 0104 chemical sciences, 3. Good health, Biochemistry, Photochemotherapy, Cancer cell, Drug delivery, Biophysics, Nanoparticles, Fluorescein, sense organs, 0210 nano-technology, Drug carrier, Lysosomes, Porosity, Camptothecin, medicine.drug
Abstract

The synthesis of mesoporous silica nanoparticles (MSN) covalently encapsulating fluoresceine or a photosensitizer, functionalized with galactose on the surface is described. Confocal microscopy experiments demonstrated that the uptake of galactose-functionalized MSN by colorectal cancer cells was mediated by galactose receptors leading to the accumulation of the nanoparticles in the endosomal and lysosomal compartments. The MSN functionalized with a photosensitizer and galactose were loaded with the anti-cancer drug camptothecin. Those MSN combining drug delivery and photodynamic therapy were tested on three cancer cell lines and showed a dramatic enhancement of cancer cell death compared to separate treatments.

Published
2012

44. Multifunctionalized mesoporous silica nanoparticles for the in vitro treatment of retinoblastoma: Drug delivery, one and two-photon photodynamic therapy

Author

Audrey Gallud, Marie Maynadier, David Brevet, Alain Morère, Youssef Mir, Cédric Rouxel, Ouahiba Hocine, Magali Gary-Bobo, Olivier Mongin, Afitz Da Silva, Jean-Olivier Durand, Mireille Blanchard-Desce, Philippe Maillard, Bernard Loock, Laurence Raehm, Marcel Garcia, Sébastien Richeter, Laboratoire d'Aquaculture tropicale, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Chimie et Photonique Moléculaires (CPM), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), Conception, synthèse et vectorisation de biomolécules. (CSVB), Institut Curie-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5), Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Paul-Valéry - Montpellier 3 (UM3), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Université Paris Descartes - Paris 5 (UPD5)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris], Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Université Paul-Valéry - Montpellier 3 (UPVM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut de Recherche pour le Développement (IRD [France-Sud]), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Université Paris Descartes - Paris 5 (UPD5)-Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université Paul-Valéry - Montpellier 3 (UPVM)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
animal structures, Retinal Neoplasms, medicine.medical_treatment, Pharmaceutical Science, Nanoparticle, Photodynamic therapy, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Cell Line, Tumor, medicine, Humans, ComputingMilieux_MISCELLANEOUS, Drug Carriers, Retinoblastoma, Chemistry, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Galactose, Mesoporous silica, Silicon Dioxide, 021001 nanoscience & nanotechnology, medicine.disease, Antineoplastic Agents, Phytogenic, Molecular biology, Endocytosis, eye diseases, In vitro, 0104 chemical sciences, 3. Good health, Photochemotherapy, Cancer cell, Drug delivery, Cancer research, Nanoparticles, Camptothecin, 0210 nano-technology, Mannose, medicine.drug
Abstract

In this work, we focused on mesoporous silica nanoparticles (MSN) for one photon excitated photodynamic therapy (OPE-PDT) combined with drug delivery and carbohydrate targeting applied on retinoblastoma, a rare disease of childhood. We demonstrate that bitherapy (camptothecin delivery and photodynamic therapy) performed with MSN on retinoblastoma cancer cells was efficient in inducing cancer cell death. Alternatively MSN designed for two-photon excited photodynamic therapy (TPE-PDT) were also studied and irradiation at low fluence efficiently killed retinoblastoma cancer cells.

Published
2012

45. Hyaluronic acid-functionalized mesoporous silica nanoparticles for efficient photodynamic therapy of cancer cells

Author

Magali Gary-Bobo, Marcel Garcia, Philippe Maillard, Nadia Benkirane-Jessel, Laurence Raehm, Jean-Olivier Durand, David Brevet, Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Biomatériaux et ingénierie tissulaire, Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital Central, Service de Chirurgie Orthopédique (UMR7561 CNRS), Inconnu, Conception, synthèse et vectorisation de biomolécules. (CSVB), Institut Curie-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), and Université Paris Descartes - Paris 5 (UPD5)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]

Subjects
animal structures, medicine.medical_treatment, Biophysics, Photodynamic therapy, Apoptosis, 02 engineering and technology, Dermatology, 010402 general chemistry, Endocytosis, 01 natural sciences, chemistry.chemical_compound, Nanocapsules, Cell Line, Tumor, Hyaluronic acid, medicine, Moiety, Humans, Pharmacology (medical), Hyaluronic Acid, ComputingMilieux_MISCELLANEOUS, biology, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Chemistry, CD44, Mesoporous silica, 021001 nanoscience & nanotechnology, Silicon Dioxide, 0104 chemical sciences, Treatment Outcome, Oncology, Biochemistry, Photochemotherapy, Cell culture, Cancer cell, biology.protein, Cancer research, sense organs, 0210 nano-technology, Colorectal Neoplasms, Porosity
Abstract

Mesoporous silica nanoparticles (MSN) for photodynamic therapy (PDT) were coated with poly-(L-lysine) and hyaluronic acid (HA) by using the layer-by-layer method. HA is able to target cancer cells over-expressing the corresponding CD44 receptor. MSN functionalized with HA (MSN-HA) were more efficient than MSN without the targeting moiety when PDT was performed at low fluence (14 Jcm(-2)) and low dosage of MSN (20 μgmL(-1)) on HCT 116 colorectal cancer cells, known to over-express the CD44 receptor. Incubation of HCT-116 cancer cells with an excess of HA impaired the PDT effect with MSN-HA thus demonstrating that an active endocytosis mechanism was involved in the uptake of MSN-HA by these cells.

Published
2011

46. Facile route to functionalized mesoporous silica nanoparticles by click chemistry

Author

Nirmalya Moitra, Xavier Cattoën, Jean-Olivier Durand, Laurence Raehm, Philippe Trens, Michel Wong Chi Man, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), and Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC)

Subjects
chemistry.chemical_classification, Materials science, Alkyne, Nanoparticle, 02 engineering and technology, General Chemistry, [CHIM.MATE]Chemical Sciences/Material chemistry, Mesoporous silica, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, Combinatorial chemistry, 0104 chemical sciences, symbols.namesake, chemistry.chemical_compound, chemistry, Materials Chemistry, symbols, Click chemistry, Organic chemistry, Surface modification, Azide, Fourier transform infrared spectroscopy, 0210 nano-technology, Raman spectroscopy
Abstract

International audience; Mesoporous silica nanoparticles containing clickable functions (azide or alkyne, 1 or 10 mol%) were synthesized by the sol-gel method directly from molecular precursors using CTAB as structure directing agent. Their structures were discussed on the basis of TEM, DLS, SAXS, N2-sorption analyses, FTIR, Raman and elemental analysis. The success of their functionalization by pyrene derivatives using CuAAC reactions opens new perspectives in the synthesis of functional MSNs for applications as nanomachines.

Published
2011

47. Nanoparticles for photodynamic therapy applications

Author

Laurence Raehm, Muriel Barberi-Heyob, Sébastien Richeter, Céline Frochot, Jean-Olivier Durand, Régis Vanderesse, Laboratoire de Chimie Physique Macromoléculaire (LCPM), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Médicaments Photoactivables - Photochimiothérapie (PHOTOMED), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Réactions et Génie des Procédés (LRGP), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche en Automatique de Nancy (CRAN), Université Henri Poincaré - Nancy 1 (UHP)-Institut National Polytechnique de Lorraine (INPL)-Centre National de la Recherche Scientifique (CNRS), Centre Alexis Vautrin (CAV), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), and Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC)

Subjects
Chemistry, medicine.medical_treatment, Nanoparticle, Photodynamic therapy, Nanotechnology, 02 engineering and technology, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, 0104 chemical sciences, 3. Good health, Radiation therapy, In vivo, Cancer cell, medicine, Nanomedicine, Photosensitizer, [SDV.IB]Life Sciences [q-bio]/Bioengineering, 0210 nano-technology
Abstract

ISBN 978-94-007-1247-8 http://www.springer.com/life+sciences/cell+biology/book/978-94-007-1247-8; Photodynamic therapy has emerged as an alternative to chemo- and radiotherapy for the treatment of certain types of cancer. Nanoparticles have been used to improve the delivery and the efficiency of the photosensitizer as they allow its encapsulation without loss of activity. Using targeting strategies, they can also allow the selective accumulation of the photosensitizer in the cancer tissues. In this review, based on the chemical nature of the nanoparticles, the different methods for their syntheses are described from the pioneering works to the latest achievements. Indeed the nanosystems can be conjugated to a biomolecule or an antibody to target receptors over-expressed in cancer cells and/or angiogenic vascular endothelial cells. Numerous in vivo and in vitro applications have been described. Multifunctional nanoplatforms combining several applications within the same nano-object emerge as potential important theranostic tools.

Published
2011

48. Mannose-Functionalized Mesoporous Silica Nanoparticles for Efficient Two-Photon Photodynamic Therapy of Solid Tumors

Author

Marie Maynadier, Laurence Raehm, Ophélie Vaillant, Jean-Olivier Durand, Cédric Rouxel, Marcel Garcia, Ilaria Basile, Youssef Mir, Magali Gary-Bobo, Olivier Mongin, David Brevet, Mireille Blanchard-Desce, Alain Morère, Institut de recherche en cancérologie de Montpellier (IRCM - U896 Inserm - UM1), Université Montpellier 1 (UM1)-CRLCC Val d'Aurelle - Paul Lamarque-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Chimie et Photonique Moléculaires (CPM), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), controle de la progression des cancers hormonaux-dependants, Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), and Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)

Subjects
medicine.medical_treatment, Mannose, Nanoparticle, Mice, Nude, Photodynamic therapy, Context (language use), Breast Neoplasms, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Catalysis, chemistry.chemical_compound, Mice, Drug Delivery Systems, Two-photon excitation microscopy, Cell Line, Tumor, medicine, Animals, Humans, ComputingMilieux_MISCELLANEOUS, Photosensitizing Agents, 010405 organic chemistry, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Cancer, General Chemistry, General Medicine, Mesoporous silica, medicine.disease, Silicon Dioxide, 021001 nanoscience & nanotechnology, Combinatorial chemistry, Xenograft Model Antitumor Assays, 0104 chemical sciences, Radiation therapy, Mannose-Binding Lectins, chemistry, Photochemotherapy, Nanoparticles, Female, 0210 nano-technology
Abstract

In the context of national systematic screenings for cancer,photodynamic therapy (PDT) has arisen as an alternative tochemo- and radiotherapy for the non-invasive selectivedestruction of small tumors. PDT involves the use of aphotosensitizer which, upon irradiation at specific wave-lengths, in the presence of oxygen, leads to the generation ofcytotoxic species and consequently to irreversible celldamage.

Published
2011

49. Mesoporous Silica Nanoparticles Combining Two-Photon Excited Fluorescence and Magnetic Properties

Author

Kalaivani Thangavel, Laurence Raehm, Elena Chelebaeva, Youssef Mir, Mireille Blanchard-Desce, Yannick Guari, Marc Willinger, Christian Guérin, A. A. Trifonov, Olivier Mongin, Alessandro Lascialfari, Joulia Larionova, Jean-Olivier Durand, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Russian Acad Sci, GA Razunaev Inst Organomet Chem, Russian Academy of Sciences [Moscow] (RAS), Universidade de Aveiro, Istituto di Fisiologia Generale e Chimica Biologica, Università degli Studi di Milano [Milano] (UNIMI), Synthèse et électrosynthèse organiques (SESO), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Università degli Studi di Milano = University of Milan (UNIMI), and Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Materials science, Coordination polymer, Analytical chemistry, Nanoparticle, 02 engineering and technology, General Chemistry, Mesoporous silica, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, 0104 chemical sciences, chemistry.chemical_compound, Chemical engineering, chemistry, Transmission electron microscopy, Materials Chemistry, Magnetic nanoparticles, [CHIM.COOR]Chemical Sciences/Coordination chemistry, 0210 nano-technology, Luminescence, High-resolution transmission electron microscopy, ComputingMilieux_MISCELLANEOUS, Superparamagnetism
Abstract

A new approach to the synthesis of multifunctional nanoparticles was developed by using covalent anchoring of cyano-bridged coordination polymer Ni2+/[Fe(CN)6]3− to the surface of two-photon dye-doped mesoporous silica nanoparticles. The obtained hybrid nanoparticles were studied by infrared (IR) spectroscopy, nitrogen adsorption (BET), X-ray diffraction, transmission electron microscopy (TEM), high resolution transmission electron microscopy (HRTEM), luminescence, and magnetic analysis. The synthesis leads to homogeneously dispersed uni-shaped nanoparticles of around 100 nm in length that are coated with cyano-bridged metallic coordination polymer nanoparticles. These hybrid nanoparticles combine effective two-photon excited fluorescence, porosity, high transverse nuclear relaxivity values (i.e. the magnetic resonance imaging efficiency) and superparamagnetic properties.

Published
2010

50. Silicalites and Mesoporous Silica Nanoparticles for photodynamic therapy

Author

Pierre Couleaud, Sébastien Richeter, Alain Morère, Magali Gary-Bobo, Ouahiba Hocine, Monique Smaïhi, Simon Fontanel, Bernard Loock, Marie Maynadier, Laurence Raehm, Gaelle Derrien, Philippe Maillard, Céline Frochot, Jean-Olivier Durand, Clarence Charnay, Amar Sahmoune, David Brevet, Marcel Garcia, Conception, synthèse et vectorisation de biomolécules. (CSVB), Université Paris Descartes - Paris 5 (UPD5)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris], Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Institut de recherche en cancérologie de Montpellier (IRCM - U896 Inserm - UM1), CRLCC Val d'Aurelle - Paul Lamarque-Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 1 (UM1), Médicaments Photoactivables - Photochimiothérapie (PHOTOMED), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Réactions et Génie des Procédés (LRGP), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Institut Européen des membranes (IEM), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Marie, Nathalie, Institut Curie-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Université Montpellier 1 (UM1)-CRLCC Val d'Aurelle - Paul Lamarque-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS), and Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
medicine.medical_treatment, Pharmaceutical Science, Nanoparticle, Mannose, Photodynamic therapy, Nanotechnology, Breast Neoplasms, Receptors, Cell Surface, 02 engineering and technology, 010402 general chemistry, Endocytosis, 01 natural sciences, chemistry.chemical_compound, Drug Delivery Systems, Cell Line, Tumor, medicine, Humans, Lectins, C-Type, Microparticle, ComputingMilieux_MISCELLANEOUS, Fluorescent Dyes, Microscopy, Confocal, Singlet Oxygen, Chemistry, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Silicates, [CHIM.MATE]Chemical Sciences/Material chemistry, Mesoporous silica, 021001 nanoscience & nanotechnology, Silicon Dioxide, [CHIM.ORGA] Chemical Sciences/Organic chemistry, 3. Good health, 0104 chemical sciences, Mannose-Binding Lectins, Photochemotherapy, Biophysics, Surface modification, Nanoparticles, Female, Fluorescein, 0210 nano-technology, Mesoporous material, Lysosomes, Mannose Receptor
Abstract

International audience; The synthesis of silicalites and Mesoporous Silica Nanoparticles (MSN), which covalently incorporate original water-soluble photosensitizers for PDT applications is described. PDT was performed on MDA-MB-231 breast cancer cells. All the nanoparticles showed significant cell death after irradiation, which was not correlated with 1O2 quantum yield of the nanoparticles. Other parameters are involved and in particular the surface and shape of the nanoparticles which influence the pathway of endocytosis. Functionalization with mannose was necessary to obtain the best results with PDT due to an active endocytosis of mannose-functionalized nanoparticles. The quantity of mannose on the surface should be carefully adjusted as a too high amount of mannose impairs the phototoxicity of the nanoparticles. Fluorescein was also encapsulated in MCM-41 type MSN in order to localize the nanoparticles in the organelles of the cells by confocal microscopy. The MSN were localized in lysosomes after active endocytosis by mannose receptors.

Published
2010

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