1. Screening, Identification and Efficacy Evaluation of Antagonistic Bacteria for Biocontrol of Soft Rot Disease Caused by Dickeya zeae
- Author
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Yang Xue, Jianuan Zhou, Ming Hu, Li Jieling, Lian-Hui Zhang, Guangtao Lu, and Chen Xia
- Subjects
0106 biological sciences ,0301 basic medicine ,Microbiology (medical) ,species identification ,determination of biocontrol effect ,Pseudomonas fluorescens ,medicine.disease_cause ,01 natural sciences ,Microbiology ,Article ,03 medical and health sciences ,Bacterial soft rot ,Virology ,Fusarium oxysporum ,medicine ,lcsh:QH301-705.5 ,Wilt disease ,biology ,Pseudomonas ,food and beverages ,Pathogenic bacteria ,biology.organism_classification ,Antimicrobial ,030104 developmental biology ,lcsh:Biology (General) ,Bacteria ,antagonistic bacterial screening ,010606 plant biology & botany - Abstract
Dickeya zeae is the causal agent of bacterial soft rot disease, with a wide range of hosts all over the world. At present, chemical agents, especially agricultural antibiotics, are commonly used in the prevention and control of bacterial soft rot, causing the emergence of resistant pathogens and therefore increasing the difficulty of disease prevention and control. This study aims to provide a safer and more effective biocontrol method for soft rot disease caused by D. zeae. The spot-on-lawn assay was used to screen antagonistic bacteria, and three strains including SC3, SC11 and 3-10 revealed strong antagonistic effects and were identified as Pseudomonas fluorescens, P. parafulva and Bacillus velezensis, respectively, using multi-locus sequence analysis (MLSA) based on the sequences of 16S rRNA and other housekeeping genes. In vitro antimicrobial activity showed that two Pseudomonas strains SC3 and SC11 were only antagonistic to some pathogenic bacteria, while strain 3-10 had broad-spectrum antimicrobial activity on both pathogenic bacteria and fungi. Evaluation of control efficacy in greenhouse trials showed that they all restrained the occurrence and development of soft rot disease caused by D. zeae MS2 or EC1. Among them, strain SC3 had the most impressive biocontrol efficacy on alleviating the soft rot symptoms on both monocotyledonous and dicotyledonous hosts, and strain 3-10 additionally reduced the occurrence of banana wilt disease caused by Fusarium oxysporum f. sp. cubensis. This is the first report of P. fluorescens, P. parafulva and B. velezensis as potential bio-reagents on controlling soft rot disease caused by D. zeae.
- Published
- 2020
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