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Screening, Identification and Efficacy Evaluation of Antagonistic Bacteria for Biocontrol of Soft Rot Disease Caused by Dickeya zeae
- Source :
- Microorganisms, Volume 8, Issue 5, Microorganisms, Vol 8, Iss 697, p 697 (2020)
- Publication Year :
- 2020
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2020.
-
Abstract
- Dickeya zeae is the causal agent of bacterial soft rot disease, with a wide range of hosts all over the world. At present, chemical agents, especially agricultural antibiotics, are commonly used in the prevention and control of bacterial soft rot, causing the emergence of resistant pathogens and therefore increasing the difficulty of disease prevention and control. This study aims to provide a safer and more effective biocontrol method for soft rot disease caused by D. zeae. The spot-on-lawn assay was used to screen antagonistic bacteria, and three strains including SC3, SC11 and 3-10 revealed strong antagonistic effects and were identified as Pseudomonas fluorescens, P. parafulva and Bacillus velezensis, respectively, using multi-locus sequence analysis (MLSA) based on the sequences of 16S rRNA and other housekeeping genes. In vitro antimicrobial activity showed that two Pseudomonas strains SC3 and SC11 were only antagonistic to some pathogenic bacteria, while strain 3-10 had broad-spectrum antimicrobial activity on both pathogenic bacteria and fungi. Evaluation of control efficacy in greenhouse trials showed that they all restrained the occurrence and development of soft rot disease caused by D. zeae MS2 or EC1. Among them, strain SC3 had the most impressive biocontrol efficacy on alleviating the soft rot symptoms on both monocotyledonous and dicotyledonous hosts, and strain 3-10 additionally reduced the occurrence of banana wilt disease caused by Fusarium oxysporum f. sp. cubensis. This is the first report of P. fluorescens, P. parafulva and B. velezensis as potential bio-reagents on controlling soft rot disease caused by D. zeae.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Microbiology (medical)
species identification
determination of biocontrol effect
Pseudomonas fluorescens
medicine.disease_cause
01 natural sciences
Microbiology
Article
03 medical and health sciences
Bacterial soft rot
Virology
Fusarium oxysporum
medicine
lcsh:QH301-705.5
Wilt disease
biology
Pseudomonas
food and beverages
Pathogenic bacteria
biology.organism_classification
Antimicrobial
030104 developmental biology
lcsh:Biology (General)
Bacteria
antagonistic bacterial screening
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 20762607
- Database :
- OpenAIRE
- Journal :
- Microorganisms
- Accession number :
- edsair.doi.dedup.....4f81d33b108cd99f812408d44fc16df9
- Full Text :
- https://doi.org/10.3390/microorganisms8050697