1. Identification of phenolic compounds in red wine extract samples and zebrafish embryos by HPLC-ESI-LTQ-Orbitrap-MS
- Author
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Mercè Mercader-Martí, Anna Vallverdú-Queralt, Rosa M. Lamuela-Raventós, Nuria Boix, Gemma Sasot, Juan M. Llobet, Alexander Medina-Remón, Jesús Gómez-Catalán, Ester Piqué, Sciences Pour l'Oenologie (SPO), Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), INSA-UB, Nutrition and Food Safety Research Institute, University of Barcelona, Centro de Investigación Biomédica en Red de la Fisiopatología de la Obesidad y Nutrición (ciberobn), Instituto de Salud Carlos III (ISC), GRET-CERETOX and Toxicology Unit, Pharmacology and Therapeutical Chemistry, Pharmacy School, Nutrition and Food Science Department, XaRTA, Pharmacy School, Miguel Torres S.A., Institute of Nutrition and Food Safety (INSA) of the University of Barcelona [FRI2012], INNPRONTA, CICYT [AGL2010-22319-C03, AGL2013-49083-C3-1-R], Instituto de Salud Carlos III, ISCIII (ciberobn) from the Spanish Ministry of Science and Innovation Ministerio de Ciencia e Innovacion (MICINN), Quality Group from Generalitat de Catalunya Generalitat de Catalunya (GC) [2014 SGR 773, SGR 566], Foundation Alfonso Martin Escudero for carrying out research in foreign countries, 'Juan de la Cierva' postdoctoral program from Ministerio de Economia y Competitividad [JCI-2012-13463], Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université Montpellier 1 (UM1)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Instituto de Salud Carlos III [Madrid] (ISC), and Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)
- Subjects
Spectrometry, Mass, Electrospray Ionization ,Identification ,Electrospray ,animal structures ,Zebrafish (ZF) ,030309 nutrition & dietetics ,[SDV]Life Sciences [q-bio] ,Wine ,Mass spectrometry ,01 natural sciences ,High-performance liquid chromatography ,Mass Spectrometry ,Analytical Chemistry ,03 medical and health sciences ,chemistry.chemical_compound ,Phenols ,Animals ,Zebrafish ,Chromatography, High Pressure Liquid ,0303 health sciences ,Orbitrap-MS ,Chromatography ,biology ,010401 analytical chemistry ,Polyphenols ,food and beverages ,Red wine extract ,General Medicine ,biology.organism_classification ,3. Good health ,0104 chemical sciences ,chemistry ,Polyphenol ,Anthocyanin ,embryonic structures ,Ion trap ,Chromatography, Liquid ,Food Science - Abstract
The zebrafish embryo is a highly interesting biological model with applications in different scientific fields, such as biomedicine, pharmacology and toxicology. In this study, we used liquid chromatography/electrospray ionisation-linear ion trap quadrupole-Orbitrap-mass spectrometry (HPLC/ESI-LTQ-Orbitrap-MS) to identify the polyphenol compounds in a red wine extract and zebrafish embryos. Phenolic compounds and anthocyanin metabolites were determined in zebrafish embryos previously exposed to the red wine extract. Compounds were identified by injection in a high-resolution system (LTQ-Orbitrap) using accurate mass measurements in MS, MS(2) and MS(3) modes. To our knowledge, this research constitutes the first comprehensive identification of phenolic compounds in zebrafish by HPLC coupled to high-resolution mass spectrometry.
- Published
- 2015
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