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Cohesion plays a critical role in chromosome segregation in both eukaryotes and E. coli, with defects leading to aneuploidy and in eukaryotes, contributing to cancer. In E. coli, SeqA regulates genomic cohesion and its loss results in segregation defect and the loss of genomic integrity. The impact of growth rate on cell phenotype and the differential proteome profile under varying growth conditions in cohesion mutants of E. coli remains unknown. In this study, we demonstrate that in nutrient-rich media, ∆seqA strain exhibits significantly increased doubling time and lower cell densities compared to wild-type strain. However, no significant differences in cell doubling time or cell density were observed in nutrient-poor media. Differential whole-proteome analysis of fast-growing ∆seqA cells revealed upregulation of genes involved in translation and chemotaxis, while stress-response genes, including those related to envelope stress and DNA damage repair, were downregulated. In contrast, during the stationary phase, differential expression was observed for genes related to protein and nucleotide metabolism. Our data shows that, during faster growth, ∆seqA mutant strains experience increased defects in chromosome segregation and DNA damage. Additionally, the reduced expression of stress-response genes results in a delayed SOS response, which leads to slower growth and increased doubling time. These findings provide valuable insights into the relationship between cohesion, segregation, growth rate and stress responses. [ABSTRACT FROM AUTHOR]

A significant number of the genetic alterations observed in cancer patients lie within nonprotein-coding segments of the genome, including regions coding for long noncoding RNAs (lncRNAs). LncRNAs display aberrant expression in breast cancer (BrCa), but the functional implications of this altered expression remain to be elucidated. By performing transcriptome screen in a triple negative BrCa (TNBC) isogenic 2D and 3D spheroid model, we observed aberrant expression of >1000 lncRNAs during BrCa progression. The chromatin-associated lncRNA MANCR shows elevated expression in metastatic TNBC. MANCR is upregulated in response to cellular stress and modulates DNA repair and cell proliferation. MANCR promotes metastasis as MANCR-depleted cells show reduced cell migration, invasion, and wound healing in vitro, and reduced metastatic lung colonization in xenograft experiments in vivo. Transcriptome analyses reveal that MANCR modulates expression and pre-mRNA splicing of genes, controlling DNA repair and checkpoint response. MANCR promotes the transcription of NET1A, a Rho-GEF that regulates DNA damage checkpoint and metastatic processes in cis, by differential promoter usage. Experiments suggest that MANCR regulates the expression of cancer-associated genes by modulating the association of various transcription factors and RNA-binding proteins. Our results identified the metastasis-promoting activities of MANCR in TNBC by cis-regulation of gene expression. [ABSTRACT FROM AUTHOR]

Introduction Glioblastoma multiforme (GBM) is a devastating form of cancer with a poor prognosis despite available treatments. Managing recurrent GBM remains challenging and lacks guidelines. This study aims to provide practice patterns for managing recurrent GBMs in India. Methods A panel of experts was assembled to develop practice patterns using the Delphi technique. Their responses were analyzed anonymously to ensure impartiality and generate recommendations. The statements were intended to be nonbinding and focused on promoting best practices in the field, without legal or regulatory authority. Results A total of 23 experts participated in the study, providing their opinions on various aspects of managing recurrent GBM. Consensus was achieved on individualized and multidisciplinary management as the preferred approach. Surgery in combination with other treatments was found to impact survival in patients older than 65 years, with re-surgery and adjuvant radiation and chemotherapy being the preferred options. Gadolinium-enhanced magnetic resonance imaging (MRI) brain with spectroscopy and diffusion-weighted imaging was favored. Molecular profiling was considered significant, with O 6 -methylguanine DNA methyltransferase methylation being most relevant. Surgery was recommended for recurrent GBMs, primarily based on Karnofsky's performance score (KPS). Surgical adjuncts such as neuronavigation and intraoperative MRI were considered valuable. Radiation therapy, specifically stereotactic radiosurgery, was recommended for selected cases, while opinions on re-chemotherapy were divided. Palliative care was deemed important. Conclusion This study presents practice patterns for managing recurrent GBM in India, providing standardized recommendations for practice. By implementing these, clinicians can make informed decisions, leading to improved patient outcomes and reduced variability in the management of recurrent GBM. [ABSTRACT FROM AUTHOR]

Sub-optimal wheat productivity in the eastern Indo-Gangetic plain of India can largely be attributed to delayed sowing and the use of short duration varieties. The second week of November is the ideal time for sowing wheat in eastern India, though farmers generally plant later. Late-sowing farmers tend to prefer short-duration varieties, leading to additional yield penalty. To validate the effect of timely sowing and the comparative performance of long- and short-duration varieties, multi-location on-farm trials were conducted continuously over five years starting from 2016–2017. Ten districts were selected to ensure that all the agro-climatic zones of the region were covered. There were five treatments of sowing windows: (T1) 1 to 10 November, (T2) 11–20 November, (T3) 21 to 30 November, (T4) 1–15 December, and (T5) 16–31 December. Varietal performance was compared in T3, T4, and T5, as short-duration varieties are normally sown after 20 November. There is asymmetry in the distribution of samples within treatments and over the years due to the allocation of fields by farmers. Altogether, the trial was conducted at 3735 sites and captured 61 variables, including yield and yield attributing traits. Findings suggested that grain yields of long-duration wheat varieties are better even under late sown scenarios. Dataset:  https://hdl.handle.net/11529/10548817 Dataset License: Creative Commons CC0 Public Domain Dedication [ABSTRACT FROM AUTHOR]

Background: Occipito-cervical fixation (OCF) provides immediate rigid fixation to cranio-vertebral junction (CVJ); however, in current practice, the optimal occipito-cervical fixation method is arguable.Aim: The aim of this study was to test the safety and efficacy of a newly designed inside-outside occipital (OC) plate system for the treatment of cranio-vertebral junction instability.Material and Methods: Thirty-two patients of CVJ instability were treated using this new OC plate system. Safety and efficacy of this new OC plate was evaluated radiologically and clinically.Results: Follow-up period ranged from 9 to 23 months. During the follow-up, no implant failure, recurrent subluxation, or newly developed instability at adjacent levels occurred, except in one patient in whom C2 screw pullout occurred due to trauma. All patients showed a satisfactory fusion at three months follow-up examination.Conclusions: These preliminary results suggest that this OC plate system is a simple, safe, and effective method for providing immediate internal rigid fixation of the CV junction. Long-term results are needed to determine the superiority of this OC plate over other methods of occipital fixation. [ABSTRACT FROM AUTHOR]

Ruthenates provide a comprehensive platform to study a plethora of novel properties, such as quantum magnetism, superconductivity, and magnetic fluctuation mediated metal–insulator transition (MIT). Herein, an overview of quantum mechanical phenomenology in calcium ruthenium oxide with varying compositions is provided. While the stoichiometric composition of CaRuO3 exhibits non‐fermi liquid (FL) behavior with quasi‐criticality, chemically doped compounds depict prominent signatures of quantum magnetic fluctuations at low temperature that in some cases are argued to mediate in metal–insulator transition. In the case of cobalt‐doped CaRuO3, an unusual continuum fluctuation is found to persist deep inside the glassy phase of the material. These observations reflect the richness of the ruthenate research platform in the study of quantum magnetic phenomena of fundamental importance. [ABSTRACT FROM AUTHOR]

Introduction: Functional improvement in lumbar PIVD patients can be assessed either objectively like improvement in SLRT, relief in pain, etc or subjectively using different types of scales. In our study, we have used Revised Oswestry Disability Index (RODI) score, Ronald-Morris disability questionnaire (RDQ), The Back Bournemouth Questionnaire (BQ) to analyse functional outcome in single and double level lumbar PIVD patients pre-operatively and post-operatively. Method: It is a prospective study including 80 patients of lumbar PIVD who failed to respond to conservative treatment. Patients were clinically evaluated and disability scales viz-RODI, RDQ & BQ were recorded. After lumber discectomy, patients were again assessed and scored as per disability scales at 1 month, 6 months and 1 year postoperatively. Result: RODI, RDQ and BQ scores were calculated at pre-operatively and postoperatively 1, 6 and 12 months and statistically analysed. The mean RODI scores at pre-operative and postoperative 1, 6, and 12 months were 72, 18, 10, and 6 respectively. The mean RDQ scores at pre-operative and postoperative 1, 6, and 12 months were 15, 5, 3, and 2 respectively. Similarly, the mean BQ scores at preoperative and postoperative 1, 6, and 12 months were 51, 12, 8, and 4 respectively. Statistically, significant improvement was seen in mean scores of all 3 functional scales and maximum changes were observed after 1 month. Statistically, significant improvements were observed in 54 out of 62 patients (87%). Three questions of BQ related to the patient social and family activities, anxiety and depression were separately compared pre and post-operatively and they showed a statistically significant improvement. Conclusion: Overall 87% of patients had a significant improvement in functional assessment using RODI, RDQ and BQ scales. On comparing single and double level discectomy patients, the functional improvement was similar in follow up of one year. Social and family activities (SFA), depression and anxiety of the patients improved significantly over 1 year. [ABSTRACT FROM AUTHOR]

Geometrically frustrated materials, such as spin ice or kagome lattice, are known to exhibit exotic Hall effect phenomena due to spin chirality. For the first time, Hall effect mechanism is explored in an artificial honeycomb spin ice of Nd–Sn element using Hall probe and polarized neutron reflectivity measurements. In an interesting observation, a strong enhancement in Hall signal at relatively higher temperature of T ∼ 20 K is detected. The effect is attributed to the planar Hall effect due to magnetic moment configuration in spin ice state in low field application. In the antiferromagnetic state of neodymium at low temperature, applied field induced coupling between atomic Nd moments and conduction electrons in underlying lattice causes distinct increment in Hall resistivity at very modest field of H ∼ 0.015 T. The experimental findings suggest the development of a new research vista to study the planar and the field induced Hall effects in artificial spin ice. [ABSTRACT FROM AUTHOR]

Abstract Background Enteric protozoa and sporozoa have emerged as important opportunistic parasites and can cause fatal infections in AIDS patients. The line of treatment being different for them necessitates an accurate and prompt identification of these to avoid empirical treatment. In this study which is the first of its kind from India we did a comprehensive evaluation of different techniques, comparing them on the basis of the attributes like yield, cost, time taken, expertise and infrastructure. For the first time combination of Calcoflour White and DAPI, a nuclear stain, were used to identify Microsporidia spp. Thus, a diagnostic protocol was devised for rapid, sensitive and cost effective identification of the opportunistic enteric protozoa. Results The organisms isolated from the stool samples of the cases (450 HIV patients) were predominantly Cryptosporidium spp., Microsporidia spp. and Cyclospora spp. Interestingly, the control group (200 relatives of the patients who were HIV negative) showed a high incidence (21%) of Cryptosporidium spp. We found a significant increase in the sensitivity of microscopy in detecting Cryptosporidium spp. and Cyclospora spp. after formol ether concentration. Kinyoun's staining was better compared to Modified safranin staining for Cryptosporidium spp. identification. Although ELISA had a sensitivity of 93.25% and specificity of 97% for Cryptosporidium spp. detection, we ranked Kinyoun's staining better than ELISA because it is not affordable to most of our patients. For detecting Cyclospora cayetanensis, autoflourescence was the easiest and most cost effective method followed by Safranin technique. Combination of Calcoflour White stain and DAPI gave good results for the identification of Microsporidia spp. We assessed the above techniques and graded the attributes in the following descending order: cost effectiveness, sensitivity, ease of use and interpretation, time taken for the procedure and batch testing. Conclusion Thus, we conclude that a combination of minimum three procedures should be carried out for the screening of stool specimens of HIV positive patients. Kinyoun's staining should be made mandatory for every diarrheal stool sample from HIV patients. Also every laboratory should assign its own value to the attributes and apply Multiattribute utility theory or the Analytical hierarchy process to decide the most appropriate methodology.

Vitamin D deficiency is a common disorder that is associated with morbidity and mortality in the general population. We conducted a cross-sectional study of 384 children admitted to paediatric intensive care to determine its prevalence and association with severity of illness and outcome in critically ill children. The severity of illness was evaluated using the paediatric risk of mortality score (PRISM III), on admission, at 24 and 48 h. Vitamin D deficiency was observed in 175 children (45.6%) and was associated with higher severity of illness, need for mechanical ventilation and increased mortality. [ABSTRACT FROM AUTHOR]

The synergistic investigation of ground-state magnetic correlation in the singlecrystal heavy fermion compound CeAuSb2 using detailed neutron scattering measurements and density functional calculations is reported. Unlike the previous reports of single antiferromagnetic transition at TN ≈ 6 K, three successive transitions with distinct critical exponents at TN = 5.3, 4.46, and 3.76 K, respectively, are detected in CeAuSb. The low-temperature ground-state magnetic correlation is described by the spin density wave order in the basal plane with propagation wave vector τ = (0.135, 0.135, 0.5) rlu. The spin density wave order arises due to the nesting of hole pockets in the Fermi surface, with parallel surfaces being separated by the experimentally found propagation vector τ. The comprehensive investigation of magnetic ground-state properties is expected to provide new insights in understanding the emerging quantum magnetism in this system, including the debated quantum critical state and magnetic field-induced metamagnetic transitions at low temperature. [ABSTRACT FROM AUTHOR]

The human genome encodes thousands of long noncoding RNA (lncRNA) genes; the function of majority of them is poorly understood. Aberrant expression of a significant number of lncRNAs is observed in various diseases, including cancer. To gain insights into the role of lncRNAs in breast cancer progression, we performed genome-wide transcriptome analyses in an isogenic, triple negative breast cancer (TNBC/basal-like) progression cell lines using a 3D cell culture model. We identified significantly altered expression of 1853 lncRNAs, including ~500 natural antisense transcript (NATs) lncRNAs. A significant number of breast cancer-deregulated NATs displayed co-regulated expression with oncogenic and tumor suppressor protein-coding genes in cis. Further studies on one such NAT, PDCD4-AS1 lncRNA reveal that it positively regulates the expression and activity of the tumor suppressor PDCD4 in mammary epithelial cells. Both PDCD4-AS1 and PDCD4 show reduced expression in TNBC cell lines and in patients, and depletion of PDCD4-AS1 compromise the cellular levels and activity of PDCD4. Further, tumorigenic properties of PDCD4-AS1-depleted TNBC cells were rescued by exogenous expression of PDCD4, implying that PDCD4-AS1 acts upstream of PDCD4. Mechanistically, PDCD4-AS1 stabilizes PDCD4 RNA by forming RNA duplex and controls the interaction between PDCD4 RNA and RNA decay promoting factors such as HuR. Our studies demonstrate crucial roles played by NAT lncRNAs in regulating post-transcriptional gene expression of key oncogenic or tumor suppressor genes, thereby contributing to TNBC progression. [ABSTRACT FROM AUTHOR]

Abstract: Quantum magnetic properties in a geometrically frustrated lattice of spin‐1/2 magnet, such as quantum spin liquid or solid and the associated spin fractionalization, are considered key in developing a new phase of matter. The feasibility of observing the quantum magnetic properties, usually found in geometrically frustrated lattice of spin‐1/2 magnet, in a perovskite material with controlled disorder is demonstrated. It is found that the controlled chemical disorder, due to the chemical substitution of Ru ions by Co‐ions, in a simple perovskite CaRuO3 creates a random prototype configuration of artificial spin‐1/2 that forms dimer pairs between the nearest and further away ions. The localization of the Co impurity in the Ru matrix is analyzed using the Anderson localization formulation. The dimers of artificial spin‐1/2, due to the localization of Co impurities, exhibit singlet‐to‐triplet excitation at low temperature without any ordered spin correlation. The localized gapped excitation evolves into a gapless quasi‐continuum as dimer pairs break and create freely fluctuating fractionalized spins at high temperature. Together, these properties hint at a new quantum magnetic state with strong resemblance to the resonance valence bond system. [ABSTRACT FROM AUTHOR]

Abstract: The magnetic analog of a semiconductor diode, demonstrating unidirectional electrical transport, is a highly desirable functionality for spintronics application, as it can play a dual role as magnetic memory device and logic element. However, creating such a functional material or device with operation ability at room temperature in the absence of any external tuning parameter, for instance a magnetic field, is a challenge till date. In this study, the finding of semiconductor diode‐type rectification in a 2D honeycomb lattice, made of an ultrasmall permalloy magnet with a typical length of ≈12 nm is reported. The unidirectional electrical transport behavior, characterized by the asymmetric colossal enhancement in differential conductivity at a modest current application of ≈10–15 µA, persists to T = 300 K in honeycomb lattice of a moderate thickness of ≈6 nm. Importantly, the unidirectional biasing arises without the application of a magnetic field with an output power, ≈30 nW, by three orders of magnitude smaller than a semiconductor junction diode. Together, these properties provide a new vista for spintronics research. [ABSTRACT FROM AUTHOR]

Abstract: The nature of magnetic correlation at low temperature in two‐dimensional artificial magnetic honeycomb lattice is a strongly debated issue. While theoretical researches suggest that the system will develop a novel zero entropy spin solid state as T → 0 K, a confirmation to this effect in artificial honeycomb lattice of connected elements is lacking. This study reports on the investigation of magnetic correlation in newly designed artificial permalloy honeycomb lattice of ultrasmall elements, with a typical length of ≈12 nm, using neutron scattering measurements and temperature‐dependent micromagnetic simulations. Numerical modeling of the polarized neutron reflectometry data elucidates the temperature‐dependent evolution of spin correlation in this system. As temperature reduces to ≈7 K, the system tends to develop novel spin solid state, manifested by the alternating distribution of magnetic vortex loops of opposite chiralities. Experimental results are complemented by temperature‐dependent micromagnetic simulations that confirm the dominance of spin solid state over local magnetic charge ordered state in the artificial honeycomb lattice with connected elements. These results enable a direct investigation of novel spin solid correlation in the connected honeycomb geometry of 2D artificial structure. [ABSTRACT FROM AUTHOR]

Antiferromagnetic system with stripe magnetic order provides a potential platform for the investigation of possible unconventional superconductivity. Here we report interesting new results that possibly indicate the development of superconductivity in chemically doped CrSb1 + δ. Electrical and magnetic measurements on CrSb1 + δ, with δ varying between 0.05 and 0.1, reveal field-dependent properties that are usually observed in a superconductor. The transition to a possible superconducting state is found to occur at T = 8.8 K. The observation of possible superconducting state in CrSb1 + δ will shed new light on the origin of the unconventional superconductivity in magnetic material with long-range antiferromagnetic stripe order. [ABSTRACT FROM AUTHOR]

The mammalian genome harbors thousands of long noncoding RNA (lncRNA) genes. Recent studies have indicated the involvement of several of these lncRNAs in the regulation of gene expression. lncRNAs play crucial roles in various biological processes ranging from epigenetic gene regulation, transcriptional control, to post-transcriptional regulation. lncRNAs are localized in various subcellular compartments, and major proportion of these are retained in the cell nucleus and could be broadly classified as nuclear-retained lncRNAs (nrRNAs). Based on the identified functions, members of the nrRNAs execute diverse roles, including providing architectural support to the hierarchical subnuclear organization and influencing the recruitment of chromatin modifier factors to specific chromatin sites. In this review, we will summarize the recently described roles of mammalian nrRNAs in controlling gene expression by influencing chromatin organization, transcription, pre-mRNA processing, nuclear organization, and their involvement in disease. [ABSTRACT FROM AUTHOR]

Hexagonal Dy0.5Y0.5MnO3, a multiferroic rare-earth manganite with geometrically frustrated antiferromag- netism, has been investigated with single-crystal neutron diffraction measurements. Below 3.4 K magnetic order is observed on both the Mn (antiferromagnetic) and Dy (ferrimagnetic) sublattices that is identical to that of undiluted hexagonal DyMnO3 at low temperature. The Mn moments undergo a spin reorientation transition between 3.4 K and 10 K, with antiferromagnetic order of the Mn sublattice persisting up to 70 K; the antiferromagnetic order in this phase is distinct from that observed in undiluted (h)DyMnO3, yielding a qualitatively new phase diagram not seen in other hexagonal rare-earth manganites. A magnetic field applied parallel to the crystallographic c axis will drive a transition from the antiferromagnetic phase into the low-temperature ferrimagnetic phase with little hysteresis. [ABSTRACT FROM AUTHOR]

Background: Enteric protozoa and sporozoa have emerged as important opportunistic parasites and can cause fatal infections in AIDS patients. The line of treatment being different for them necessitates an accurate and prompt identification of these to avoid empirical treatment. In this study which is the first of its kind from India we did a comprehensive evaluation of different techniques, comparing them on the basis of the attributes like yield, cost, time taken, expertise and infrastructure. For the first time combination of Calcoflour White and DAPI, a nuclear stain, were used to identify Microsporidia spp. Thus, a diagnostic protocol was devised for rapid, sensitive and cost effective identification of the opportunistic enteric protozoa. Results: The organisms isolated from the stool samples of the cases (450 HIV patients) were predominantly Cryptosporidium spp., Microsporidia spp. and Cyclospora spp. Interestingly, the control group (200 relatives of the patients who were HIV negative) showed a high incidence (21%) of Cryptosporidium spp. We found a significant increase in the sensitivity of microscopy in detecting Cryptosporidium spp. and Cyclospora spp. after formol ether concentration. Kinyoun's staining was better compared to Modified safranin staining for Cryptosporidium spp. identification. Although ELISA had a sensitivity of 93.25% and specificity of 97% for Cryptosporidium spp. detection, we ranked Kinyoun's staining better than ELISA because it is not affordable to most of our patients. For detecting Cyclospora cayetanensis, autoflourescence was the easiest and most cost effective method followed by Safranin technique. Combination of Calcoflour White stain and DAPI gave good results for the identification of Microsporidia spp. We assessed the above techniques and graded the attributes in the following descending order: cost effectiveness, sensitivity, ease of use and interpretation, time taken for the procedure and batch testing. Conclusion: Thus, we conclude that a combination of minimum three procedures should be carried out for the screening of stool specimens of HIV positive patients. Kinyoun's staining should be made mandatory for every diarrheal stool sample from HIV patients. Also every laboratory should assign its own value to the attributes and apply Multiattribute utility theory or the Analytical hierarchy process to decide the most appropriate methodology. [ABSTRACT FROM AUTHOR]

Prostate cancer has a variable clinical course, ranging from curable local disease to lethal metastatic spread. Eradicating metastatic cells is a unique challenge that is rarely met with the available therapies. Thus, targeting prostate cancer cells in earlier disease states is a crucial window of opportunity. Interestingly, cancer cells migrate from their primary site during pre-cancerous and malignant phases to seed secondary organs. These cells, known as disseminated cancer cells (DCCs), may remain dormant for months or decades before activating to form metastases. Bone marrow, a dormancy-permissive site, is the major organ for housed DCCs and eventual metastases in prostate cancer. The dynamic interplay between DCCs and the primary tumor microenvironment (TME), as well as that between DCCs and the secondary organ niche, controls the conversion between states of dormancy and activation. Here, we discuss recent discoveries that have improved our understanding of dormancy signaling and the role of the TME in modulating the epigenetic reprogramming of DCCs. We offer potential strategies to target DCCs in prostate cancer. [ABSTRACT FROM AUTHOR]