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Jiangsu High-Tech Fluorochemical Industrial Park in Changshu City, Jiangsu Province, is the largest fluorochemical industrial park in Asia. The occurrence of per- and polyfluoroalkyl substances (PFASs) in surface water and widespread local plants was investigated in Jiangsu High-Tech Fluorochemical Industrial Park. Thirty-two target PFASs were detected in dissolved-phase, particle-phase and plant samples. The concentrations of total PFASs ranged from 1650 to 8250 ng/L in the dissolved-phase samples, 132 to 6810 ng/g dw in the particle-phase samples and 25.8 to 9460 ng/g dw in different plant tissues. Perfluorohexanoic acid (PFHxA), Perfluorooctanoate acid (PFOA) and 6:2 fluorotelomer carboxylic acid (6:2 FTCA) were predominant PFASs and contributed 80−91% to ΣPFAS in water samples. A total of 67 emerging PFAS were identified in all samples using nontargeted analysis. Typha orientalis showed better accumulation ability, with an average ΣPFAS concentration of 3450 ng/g dw and the highest root concentration factor (RCF) of 171. Typha orientalis, Eichhornia crassipes and Alternanthera sessilis have potential for use in PFAS phytostabilization. [ABSTRACT FROM AUTHOR]

Per- and polyfluoroalkyl substances (PFASs) have attracted significant attention as a emerging environmental contaminant. The biological toxicities and ecological health risks associated with PFASs have emerged as a popular subject for ecotoxicology related research. PFASs have a detrimental impact on individual microbes in the environment. Furthermore, prolonged exposure to PFASs can disrupt the composition, structure and enzyme activity of the microbial community in the environment, disrupting the normal cycling of materials and energy in the ecosystem. This paper reviewed the impact of PFASs on microbial community structure and diversity across various environmental media, namely freshwater, marine and soil. Additionally, it summarizes the effects of enzyme activity and function in response to PFASs. Based on these findings, an assessment of the future direction of ecological risk assessment of PFASs was conducted to provide a theoretical basis and support for unbiased environmental and ecological risk assessment of PFASs. [ABSTRACT FROM AUTHOR]

• Twenty-one PFASs levels in human serum, blood and urine of patients with liver diseases were analyzed. • Higher total PFAS concentrations were detected in hepatocellular carcinoma (HCC) patients than in non-HCC patients. • Significant correlation between PFAS concentrations in patients and their demographic characteristics (including gender, age, and BMI) were found. • Serum PFNA and PFBS concentrations were associated with elevated liver function biomarkers ALP and GGT. Studies have shown that per- and polyfluoroalkyl substances (PFASs) may be hepatotoxic in animals or humans. However, data on clinical epidemiology are very limited. In this study, 21 PFASs were determined in patients with liver diseases, with the highest median concentrations detected in the serum sample (26.7 ng/mL), followed by blood (10.7 ng/mL) and urine (5.02 ng/mL). Higher total PFAS concentrations were found in hepatocellular carcinoma (HCC) patients compared to non-HCC patients, with significant discrepancies in serum and blood samples. Besides, significant correlations were also found among PFAS concentrations and age, gender, body mass index (BMI), and liver function biomarkers levels. For example, PFAS concentrations are significantly higher in males than in females; Several serum PFASs concentrations increase with age and BMI, while the serum perfluorohexane sulfonic acid (PFHxS) concentrations are negatively correlated with age. In addition, multiple regression models adjusted for age, gender and BMI found that increased serum perfluorobutane sulfonic acid (PFBS), perfluoroheptane sulfonic acid (PFHpS) and perfluorohexylphosphonic acid (PFHxPA) conentrations are correlated with elevated alkaline phosphatase (ALP), aspartate aminotransferase (AST), and alpha-fetoprotein (AFP) (p < 0.05). Our results provide epidemiological support for the future study on the potential clinical hepatotoxicity of PFAS. [Display omitted] [ABSTRACT FROM AUTHOR]

In contrast to some low-molar-mass per- and polyfluoroalkyl substances (PFASs), which are well established to be toxic, persistent, bioaccumulative, and mobile, fluoropolymers (FPs) are water-insoluble, safe, bioinert, and durable. These niche high-performance polymers fulfil the 13 polymer-of-low-concern (PLC) criteria in their recommended conditions of use. In addition, more recent innovations (e.g., the use of non-fluorinated surfactants in aqueous radical (co)polymerization of fluoroalkenes) from industrial manufacturers of FPs are highlighted. This review also aims to show how these specialty polymers endowed with outstanding properties are essential (even irreplaceable, since hydrocarbon polymer alternatives used in similar conditions fail) for our daily life (electronics, energy, optics, internet of things, transportation, etc.) and constitute a special family separate from other "conventional" C1–C10 PFASs found everywhere on Earth and its oceans. Furthermore, some information reports on their recycling (e.g., the unzipping depolymerization of polytetrafluoroethylene, PTFE, into TFE), end-of-life FPs, and their risk assessment, circular economy, and regulations. Various studies are devoted to environments involving FPs, though they present a niche volume (with a yearly production of 330,300 t) compared to all plastics (with 460 million t). Complementary to other reviews on PFASs, which lack of such above data, this review presents both fundamental and applied strategies as evidenced by major FP producers. [ABSTRACT FROM AUTHOR]

This study investigates the association between prenatal exposure to per- and polyfluoroalkyl substances (PFASs) and the incidence and frequency of respiratory tract infections (RTIs) in preschool children. We selected 527 mother–infant pairs from Wuhan Healthy Baby Cohort (WHBC), China. Ten PFASs were measured in umbilical cord serum, and we collected data on common RTIs in preschool children aged 4 years through a questionnaire. Associations of single PFASs with the incidence and frequency of RTIs were analyzed via Logistic regression and Poisson regression, while the collective effect was assessed by weighted quantile sum (WQS) regression. Furthermore, stratified and interaction analyses were performed to evaluate if there were sex-specific associations. We found a positive correlation between perfluorododecanoic acid (PFDoDA) and the incidence of tonsillitis, with several PFASs also showing positive associations with its frequency. Moreover, perfluorotridecanoic acid (PFTrDA) showed a positive link with the frequency of common cold. The results of WQS regression revealed that after adjusting for other covariates, PFASs mixture showed a positive association with the incidence of tonsillitis, the frequency of common cold, and episodes. In particular, perfluoroundecanoic acid (PFUnDA), PFDoDA, PFTrDA, perfluorodecanoic acid (PFDA) and 8:2 chlorinated polyfluorinated ether sulfonic acid (8:2 Cl-PFESA) had the most significant impact on this combined effect. The results suggest that both single and mixed exposures to PFASs may cause RTIs in preschool children. However, there was no statistically significant interaction between different PFASs and sex. [ABSTRACT FROM AUTHOR]

Per- and polyfluoroalkyl substances (PFASs) are an emerging group of persistent organic pollutants in aquatic environments with high levels of toxicity and bioaccumulation. The risks posed by PFASs to the environment and health have attracted increasing attention. To remove them from water, advanced oxidation processes (AOPs), with the merits of high efficiency and low cost, are mainly used. Photo/electrocatalytic heterogeneous AOPs, with the assistance of nanostructured catalysts and external energy in the form of light/electricity, have emerged as one of the most powerful techniques, overcoming the difficulty associated with defluorination and achieving the effective and complete degradation of PFASs in water. The structures of photo/electrocatalysts play a critical role in the production of reactive oxygen species, the electron transfer process, and the degradation pathway and its efficiency. Herein, to elucidate the structure–performance relationship, a review of photo/electrocatalysts for the enhanced degradation of PFASs in heterogeneous AOPs, organized according to their composition and nanostructure design, is provided. This review article is mainly focused on (1) the mechanisms and pathways of PFAS degradation by heterogeneous photo/electrocatalytic AOPs, and (2) the structural designs and modifications of photo/electrocatalysts for the enhanced degradation of PFASs by heterogeneous AOPs. Finally, the challenges and prospects for future research into photo/electrocatalysts of heterogeneous AOPs in the field of PFAS remediation are discussed. [ABSTRACT FROM AUTHOR]

A critical examination of the occurrence of PFASs in wastewater, surface water, groundwater, and drinking water is presented. Given the ongoing shift towards substitutes of legacy PFASs and the rising role of China as global leader in PFASs production, background and hotspot concentrations of PFASs in the aquatic environment of China and Western Countries (mostly the E.U., the U.S., and Canada) are reported and critically compared for the first time, with special attention to short-chain perfluoroalkyl acids, novel substitutes like perfluoroether carboxylic and sulphonic acids, and emerging PFASs like 6:2 FTAB. Data on PFASs concentrations in the examined matrices show that i) concentrations of total PFASs and of selected perfluoroalkyl acids (PFAAs) are generally higher in China, except for PFOS, and that ii) short-chain and novel PFASs are replacing their long-chain homologues but are rarely monitored. Recommendations are provided to help prioritize the PFASs to be investigated that can end up in the environment.

Per- and polyfluoroalkyl substances (PFASs) are potentially neurotoxic compounds. Levels of PFASs in cerebrospinal fluid (CSF) could directly reflect their potential harm to the central nervous system. Because of the variety of PFASs and the rarity of CSF, there is an urgent need to establish a rapid online method to detect a broad spectrum of PFASs accurately and simultaneously by consuming a small amount of CSF. In this study, we developed a fast and automated method to analyze 52 PFASs in human CSF samples using online TurboFlow ultra-high-performance liquid chromatography–tandem mass spectrometry. Our method offered excellent matrix-matched standard curve linearity (correlation coefficient > 0.99), good limits of quantitation (MLOQs) (0.01 to 0.08 ng mL−1), satisfactory accuracy (recoveries of 74.6%–119.1%) and precision (relative standard deviations of 1.4%–13.2%), small sample amount consumption (50 μL), and fast analysis time (18 min per sample) without complex sample pretreatment procedures. These are advantageous for the high throughput screening of PFASs in environmental epidemiology studies. Repeated freeze–thaw experiments showed that it was better to perform the analytical process soon as possible after sample collection. The established method was used to analyze PFASs in 60 people. Short-chain PFASs, perfluorobutanoic acid (PFBA), perfluoropentanoic acid (PFPeA), and novel PFASs [sodium 2-(N-ethylperfluorooctane-1-sulfonamido)ethyl phosphate (SAmPAP), perfluoroethylcyclohexanesulfonate (PFECHS), and perfluoro-3, 7-dimethyloctanoic acid (P37DMOA)] were reported in CSF for the first time. PFBA and PFPeA were detected in all samples with mean concentrations of 0.24 and 0.22 ng mL−1, respectively. We also calculated the blood–brain barrier transmission efficiency of PFASs (RPFAS), and the mean RPFBA value was above 1, which indicated that PFBA might transfer from serum to CSF. [ABSTRACT FROM AUTHOR]

The Greenlandic population is highly exposed to persistent organic pollutants (POPs) through the consumption of traditional marine food, including marine mammals. Central to Greenland's economy and cultural identity, the fishing industry employes about 15% of the working population. This study investigated POP exposure, including polychlorinated biphenyls (PCBs), organochlorine pesticides (OCPs), and per- and polyfluoroalkyl substances (PFASs), among seafood processing workers at the Greenlandic west coast. We examined determinants for the POPs including age, smoking habits, ethnicity, and working place. Additionally, we explored the association between POPs and the prevalence of asthma, allergy, and lung function. With samples taken during 2016–2018, the study encompassed 382 workers, primarily of Inuit descent (93%), employed across three large factories located in Nuuk, Sisimiut, and Ilulissat, four smaller factories in settlements (Kangaatsiaq, Ikerasaarsuk, Sarfannguaq, Qeqertarsuaq), and four factory trawlers. Data collected include clinical examinations, questionnaires on ethnicity, occupational exposure status, health indicators, and smoking habits, and serum selenium and POP analyses. We used ANCOVA with adjustment for relevant confounders to assess differences in POPs between groups (e.g. ethnic groups and working place), and multiple linear and logistic regressions were used to assess associations between POPs and lung function, allergy and asthma. Significant differences in POPs were observed among ethnic groups; Faroese workers had the highest concentrations of lipophilic POPs (lipPOPs; PCBs and OCPs), while Inuit workers exhibited highest PFASs. All subsequent analyses were focused on the Inuit workers (n = 337). The PFASs were significantly higher in workers at small factories, followed by large factories and trawlers, whereas no differences were seen for lipPOPs. The differences between the working places were most likely due to differences in lifestyle and diet, but occupational exposures cannot be excluded. LipPOP and PFAS concentrations associated positively with selenium, and PFASs positively associated with lung function. However, upon adjustment of selenium, the associations between PFASs and lung function became non-significant and attenuated towards null. No significant associations were found between POPs and the prevalence of asthma or allergy. Compared to the general population in the same area and period, the seafood processing workers exhibited 2–6 times higher POP levels. The higher exposure level among seafood processing workers, as well as the difference across workplaces, underscore the need for further investigation of environmental and occupational sources of POPs in this population. These findings may contribute to future public health strategies and regulatory measures to reduce POP exposure in Arctic populations. [Display omitted] • Higher POP levels in seafood workers than the general population. • POPs differed by ethnicity; Faroese highest in lipophilic POPs and Inuit highest in PFASs. • PFAS levels higher in workers at small factories than large factories and trawlers. • POP levels were not significantly associated with asthma or allergy. [ABSTRACT FROM AUTHOR]

Experimental evidences have suggested that Per- and polyfluoroalkyl substances (PFASs) were hepatotoxicity, but epidemiologic inconsistencies. There were 1751 participants included in this study after excluding chronic hepatitis, cirrhosis, excessive alcohol drinkers, and those with missing key variables. Totally 30 PFASs were quantified using ultrahigh-pressure liquid chromatography tandem mass spectrometer (UPLC-MS). Metabolic dysfunction-associated steatotic liver disease (MASLD) defined as the presence of hepatic steatosis diagnosed on abdominal B-ultrasound in conjunction with at least one cardiometabolic risk factors (CMRF) and without other discernible cause. After multivariate adjustment, perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), perfluoroalkyl carboxylates (ΣPFCAs), and perfluoroheptanoic acid (PFHpA) were positively associated with the risk of MASLD. Specifically, for each natural log-transformed unit increase in PFOA, PFNA, and ΣPFCAs, the risk of MASLD increased by 27% (95% confidence interval (CI): 1.09–1.48), 10% (95% CI: 0.99–1.23), and 29% (95% CI: 1.09–1.53), respectively. Compared with those in Tertile 1 of PFOA, PFNA, and ΣPFCAs, the risk of MASLD was increased by 35% (95% CI: 1.06–1.71, P trend = 0.019), 46% (95% CI: 1.15–1.85, P trend = 0.0018), and 43% (95% CI: 1.13–1.82, P trend = 0.0032) in Tertile 3, respectively. For PFHpA (detection rate: 14.79%), individuals with PFHpA levels above the detection limit had increased risk of MASLD by 54% (95% CI: 1.17–2.01) compared with those with PFHpA levels below the detection limit. While 8:2 chlorinated polyfluoroethersulfonic acid (8:2 Cl-PFESA) was inversely associated with steatotic liver disease (SLD) combined with 4 or 5 CMRFs (odds ratio per ln-unit = 0.87, 95% CI: 0.77–0.99). Mixed exposure analysis showed PFNA manifested a significant positive effect, while PFUdA had a significant negative effect. No association was found between other PFASs and MASLD prevalence. More prospective studies are needed to validate our conclusions. [Display omitted] • PFOA, PFNA, ΣPFCAs, and PFHpA were positively associated with the risk of MASLD. • The associations were robust in MASLD cases with more serious metabolic disorders. • The 8:2 Cl-PFESA levels were inversely associated with SLD combined with 4 or 5 CMRFs. [ABSTRACT FROM AUTHOR]

Anion exchange resins (AERs) are promising adsorbents for removing perfluoroalkyl acids (PFAAs). However, little is known about competitive effects of specific co-solutes at typical drinking water concentrations. Therefore, this study investigated the adsorption of eight PFAAs (1–7 perfluorinated carbons) on three commercial AERs (A111, M600, PSR2Plus) with different functional groups, and examined competitive effects of chloride and sulphate at varying concentrations. Using regression modelling and the stoichiometric breakthrough model, PFAA breakthroughs in fixed-bed filters were simulated, dependent on the concentration of inorganic anions. Generally, the adsorption of PFAAs increased with increasing number and length of alkyl moieties in the functional resin group in the order of dimethylamines (A111) < dimethylethanolamines (M600) < tributylamines (PSR2Plus). Adsorption on PSR2Plus was much less inhibited by the presence of inorganic anions compared to the other resins. Depending on the water matrix and AER used, the relative residual concentration of perfluorocarboxylates as a function of the number of alkyl carbons in the molecule decreased logarithmically, with adjusted r2 ≥ 0.93 and slopes between −0.25 and −0.65 log units per additional carbon. When sulphate and chloride were present simultaneously, sulphate had a stronger inhibitory effect on the adsorption of PFAAs on all resins. Furthermore, a double logarithmic correlation was found between the adsorption of PFAAs and the concentrations of inorganic anions. The results and procedures presented here can be used by water utilities, scientific consultants, and researchers to facilitate the informed selection of AERs for PFAA adsorption and their practical application in fixed-bed filters. [Display omitted] • Adsorption of ultra-short chain; short chain PFAAs onto anion exchange resins. • PFAA adsorption and inorganic anion concentrations correlate double-logarithmically. • PFAA adsorption inhibited by SO 4 2–/Cl– in the order of A111 > M600 > PSR2Plus. • A tool for resin selection for PFAA removal at specific SO 4 2–/Cl– concentrations. • Predicting stoichiometric PFAA breakthroughs at specific SO 4 2–/Cl– concentrations. [ABSTRACT FROM AUTHOR]

This study investigates the association between prenatal exposure to per- and polyfluoroalkyl substances (PFASs) and the incidence and frequency of respiratory tract infections (RTIs) in preschool children. We selected 527 mother–infant pairs from Wuhan Healthy Baby Cohort (WHBC), China. Ten PFASs were measured in umbilical cord serum, and we collected data on common RTIs in preschool children aged 4 years through a questionnaire. Associations of single PFASs with the incidence and frequency of RTIs were analyzed via Logistic regression and Poisson regression, while the collective effect was assessed by weighted quantile sum (WQS) regression. Furthermore, stratified and interaction analyses were performed to evaluate if there were sex-specific associations. We found a positive correlation between perfluorododecanoic acid (PFDoDA) and the incidence of tonsillitis, with several PFASs also showing positive associations with its frequency. Moreover, perfluorotridecanoic acid (PFTrDA) showed a positive link with the frequency of common cold. The results of WQS regression revealed that after adjusting for other covariates, PFASs mixture showed a positive association with the incidence of tonsillitis, the frequency of common cold, and episodes. In particular, perfluoroundecanoic acid (PFUnDA), PFDoDA, PFTrDA, perfluorodecanoic acid (PFDA) and 8:2 chlorinated polyfluorinated ether sulfonic acid (8:2 Cl-PFESA) had the most significant impact on this combined effect. The results suggest that both single and mixed exposures to PFASs may cause RTIs in preschool children. However, there was no statistically significant interaction between different PFASs and sex.

Bioindicators can provide pollution information with longer temporal duration and larger spatial scale. It is an ideal strategy for long-term monitoring of bioaccumulative contaminants. Bioindicator monitoring has been widely used; however, there were seldom detailed studies about bioindicator methodology in literature. The present study proposed a bioindicator framework suitable for the local conditions of China, including selection of bioindicator species, evaluation of impact factors, and derivation of threshold values using per- and polyfluoroalkyl substances (PFASs) as an example. The criteria that proper bioindicator species should meet and the procedure how the bioindicator species is selected were proposed, under which crucian carp (Carassius auratus) was selected as the local bioindicator for studied PFASs. Several factors which may affect accumulation of contaminants in bioindicators were suggested to produce reliable and comparable results. Derivation method of bioindicator thresholds for ecological risk assessment of aquatic ecosystems was firstly developed. The long-term and short-term ecological thresholds of perfluorooctanesulfonate using crucian carp as bioindicator are 3.329 and 1.402 μg/g wet weight respectively. Using the long-term thresholds derived from chronic toxicity data and the accumulative concentrations of contaminants obtained by bioindicator results, the bioindicator monitoring can be used for long-term ecological risk surveillance. The threshold derivation method can extend the application of bioindicator monitoring from the occurrence study to ecological risk surveillance, which is especially important for China who has made progresses on regular contaminant control and starts to be concerned about the ecological risks of the emerging contaminants. The framework can be used to create national and regional long-term freshwater bioindicator monitoring programs, with the purposes of ecological risk assessment, occurrence and temporal trend study, pollution source identification, international convention fulfillment, retrospective study, etc. The bioindicator framework will benefit the aquatic environmental safety and the hazardous chemical management in China. [ABSTRACT FROM AUTHOR]

Many studies have reported that prenatal exposure to Per - and Polyfluoroalkyl Substances (PFASs) can disrupt immune function. However, little is known about the effects of PFASs on immune molecules. The study analyzed the association between prenatal exposure to mixed and single PFASs and plasma immune molecules in three-year-old children. Ten PFASs were measured in umbilical cord serum, while peripheral blood samples were collected at age three to measure immune molecules. Associations between exposure to individual and combined PFASs and immune molecules were analyzed using Generalized Linear Models and Weighted Quantile Sum (WQS) regression. (1) Interleukin-4 (IL-4) increased by 23.85% (95% CI:2.99,48.94) with each doubling of Perfluorooctanoic Acid (PFOA), and Interleukin-6 (IL-6) increased by 39.07% (95%CI:4.06,85.86) with Perfluorotridecanoic Acid (PFTrDA). Elevated PFOA and Perfluorononanoic Acid (PFNA) were correlated with increases of 34.06% (95% CI: 6.41, 70.28) and 24.41% (95% CI: 0.99, 53.27) in Eotaxin-3, respectively. Additionally, the doubling of Perfluorohexane Sulfonic Acid (PFHxS) was associated with a 9.51% decrease in Periostin (95% CI: −17.84, −0.33). (2) The WQS analysis revealed that mixed PFASs were associated with increased IL-6 (β = 0.37, 95%CI:0.04,0.69), mainly driven by PFTrDA, PFNA, and 8:2 Chlorinated Perfluoroethyl Sulfonamide (8:2 Cl-PFESA). Moreover, mixed PFASs were linked to an increase in Eotaxin-3 (β = 0.32, 95% CI: 0.09,0.55), primarily influenced by PFOA, PFTrDA, and Perfluorododecanoic Acid (PFDoDA). Prenatal PFASs exposure significantly alters the levels of immune molecules in three-year-old children, highlighting the importance of understanding environmental impacts on early immune development. [Display omitted] • PFASs were measured in umbilical cord serum, immune molecules in three-year-old. • Study links prenatal PFASs to plasma immune molecules in healthy children. • First to explore PFASs' effect on Eotaxin-3 and Periostin levels. • Mixed PFASs exposure increases the levels of IL-6 and Eotaxin-3. • Single PFASs exposure increases IL-4, IL-6, Eotaxin-3, and decreases Periostin. [ABSTRACT FROM AUTHOR]

Per- and polyfluoroalkyl substances (PFASs) are artificial persistent organic pollutants ubiquitous in ecosystem, and their bioaccumulation and adverse outcomes in plants have attracted extensive concerns. Here, we review the toxic effects of PFASs encountered by various plants from physiological, biochemical and molecular perspectives. The exposure routes and bioaccumulation of PFASs in plants from contaminated sites are also summarized. The bioaccumulation of PFASs in plants from contaminated sites varied between ng/g and μg/g levels. The 50% inhibition concentration of PFASs for plant growth is often several orders of magnitude higher than the environmentally relevant concentrations (ERCs). ERCs of PFASs rarely lead to obvious phenotypic/physiological damages in plants, but markedly perturb some biological activities at biochemical and molecular scales. PFAS exposure induces the over-generated reactive oxygen species and further damages plant cell structure and organelle functions. A number of biochemical activities in plant cells are perturbed, such as photosynthesis, gene expression, protein synthesis, carbon and nitrogen metabolisms. To restore the desire states of cells exposed to PFASs, plants initiate several detoxifying mechanisms, including enzymatic antioxidants, non-enzymatic antioxidants, metallothionein genes and metabolic reprogramming. Future challenges and opportunities in PFAS phytotoxicity studies are also proposed in the review.

Background: Over the last decade, per- and polyfluoroalkyl substances (PFASs) have become one of the most heavily investigated persistent organohalogen compound class of environmental concern. However, knowledge about their toxicology is still scarce, although PFASs as individual compounds and their industrial mixtures were shown to exert effects on the thyroid hormone system. Methods: In vitro toxicity potency factors were established for thyroid hormone transport disruption potential using the novel TTR-TRβ CALUX® bioassay for major PFASs. We assessed technical PFASs mixtures, including aqueous film-forming foam (AFFF) surfactants and chromium mist suppressants (CMS) applications with and without total oxidizable precursor (TOP) by TTR-TRβ CALUX® assay for their thyroid hormone transport disrupting potential. Results: All PFASs listed in the German guideline for drinking water (German Environment Agency, 2017) affected the T4 binding to TTR, an important plasma thyroid hormone transport protein. For all tested PFASs, potency factors based on PC80 values relative to PFOA could be obtained and ranged between PFBA (0.0018) and PFOS (2.0). Applying in vitro potency factors obtained from the present in vitro TTR-TRβ CALUX® assay study and recently reported in vivo potency factors (Zeilmaker et al., 2018; Bil et al., 2021) on the above-mentioned German guideline for PFAS in drinking water, showed that the cumulative effect-based trigger values (in vivo and in vitro) are comparable (3.0 vs. 2.9 to 4.6 μg PFOA-EQ/l). Additionally, AFFF surfactants and CMS with and without TOP assay were tested. Highest activities were found in the older AFFF surfactants (2013/2014) due to higher PFOS/PFOA levels, which were already substituted with 6:2 FTS in 2019, resulting in much lower PFOA-EQ levels. As expected also the PFOA-EQ levels increased in the samples with TOP treatment compared to the original AFFF surfactants and CMS as confirmed here by biological and chemical PFOA-equivalents (PFOA-EQ) analysis.Additionally, CMS (which have been used in the electroplating chromium industry since the 1950s) as well as PFOS-free, but not PFAS-free fume suppressants (such as Fumetrol® 21) have been tested in the TTR-TRβ CALUX® assay and showed much lower activity levels then the AFFFs, confirmed by the similar potency determination based on chemical PFASs analysis followed by transformation to PFOA-EQ for comparison. The potency factor of 6:2 FTS, which is the main substitute for PFOS in CMS, indicates that it is approximately 100-times less potent as a thyroid hormone disruptor as compared to PFOS. Conclusion: Potency factors based on PC80 values from TTR-TRβ CALUX® relative to PFOA have been developed for major PFASs. In AFFF surfactants and CMS a trend of higher activities with higher amounts of PFOS and PFOA have been found. PFOA and PFOS showed high responses in the TTR-TRβ CALUX® assay and had the largest contributions to the PFOA-EQs in the AFFF surfactants and CMS applications. Using potency factors as determined in the TTR-TRβ CALUX® to convert PFASs assessed by chemical analysis to PFOA-EQ led to comparable results as compared to the results from PFASs measured directly by the TTR-TRβ CALUX® assay. This study supports the claim that semiquantitative effect- and group-based in vitro CALUX bioanalysis tools can be applied effectively to assess industrial products containing complex mixtures with PFAS compounds for which no instrumental analysis are established, and for many compounds where in vitro toxicity data are not yet available.

Per- and polyfluoroalkyl substances (PFASs) are exceptionally stable chemicals due to their strong CF bonds. Nanoscale zero-valent iron (nZVI) particles have the potential to remove and degrade PFASs through redox activity. In this study, we deposited nZVI onto two-dimensional reduced graphene oxide (rGO) nanosheets and tested these synthesized rGO-nZVI nanohybrid (NH) for the treatment of a mixture of short- and long-chain PFASs in water with and without H2O2. All PFASs were removed at a higher efficiency by the rGO-nZVI NH than by the parent materials rGO and nZVI. Notably, the long-chain PFASs were removed at a faster rate than the short-chain PFASs. After a 10 min exposure to the rGO-nZVI NH without H2O2, the long-chain PFASs (perfluorooctane sulfonic acid (PFOS) and perfluorooctanoic acid (PFOA)) were removed by 85 % and 39 %, respectively, while short-chain PFASs (perfluoropentane sulfonic acid and perfluoropentanoic acid) were removed by 19 % and 18 %, respectively. The addition of H2O2 enhanced the PFAS treatment performance by 10–18 %, which can be attributed to the generation of reactive oxygen species by the rGO-nZVI NH. Liquid chromatography high-resolution mass spectrometry analysis confirmed the formation of unique shorter chain and partially defluorinated PFAS-Fe complexes from both PFOS and PFOA.

• High level of PFOA was found in fluoropolymer raw materials and products made in China. • The concentration of PFOA in fluoropolymer raw materials was higher than products. • Hexafluoropropylene oxide oligomers have been heavily used to substitute PFOA in fluoropolymer manufacture in China. • HFPO-TrA, a PFOA substitute, was found in high level in some emulsion samples. Perfluorooctanoic acid (PFOA), its salts, and related compounds were listed as new persistent organic pollutants by the Stockholm Convention in 2019. In this study, the occurrence of residues of PFOA and other per- and polyfluoroalkyl substances (PFASs) in raw materials and fluoropolymer products from the Chinese fluoropolymer industries are reported for the first time. The PFOA concentrations in raw materials and fluoropolymer products were in the range of 6.7 to 1.1 × 106 ng/g, and

Congenital heart disease (CHD) is the most prevalent congenital malformation worldwide, and the association between per- and polyfluoroalkyl substances (PFASs) exposure and CHD in population has only received limited study. Therefore, we conducted a multicenter case-control study to explore the associations between prenatal exposure to individual PFASs, and also a PFAS mixture, and CHD risk, including 185 CHDs and 247 controls in China from 2016 to 2021. Thirteen PFASs in maternal plasma were quantified using liquid chromatography-tandem mass spectrometry. Logistic regression and two multipollutant models (Bayesian kernel machine regression [BKMR] and quantile g-computation [qgcomp]) were used to assess the potential associations between any individual PFAS, and also a PFAS mixture, and CHD risk. After adjusting for potential confounders, logistic regression indicated significant associations between elevated levels of perfluorononanoic acid (odds ratio [OR]= 1.30, 95% confidence intervals [CI]: 1.07–1.58), perfluorodecanoic acid (OR=2.07, 95%CI: 1.32–3.26), and perfluoroundecanoic acid (OR=2.86, 95%CI:1.45–5.65) and CHD risk. The BKMR model and qgcomp approach identified that a significant positive association between the PFAS mixture and risk for CHD. These findings provide essential evidence that there is indeed a health crisis associated with PFASs and that it is linked to CHD. [Display omitted] • Prenatal exposure to individual PFASs and a PFAS mixture was associated with increased risk for CHD. • 6:2 chlorinated polyfluorinated ether sulfonic acid was significantly linked to an elevated risk for complex CHD. • PFAS exposure had a stronger association with CHD in males than in females. [ABSTRACT FROM AUTHOR]

The vast number of chemicals potentially reaching aquatic environment pose a challenge in maintaining the quality of water resources. However, best management practices to improve water quality are typically focused on reducing nutrient transport without assessing how these practices may impact the occurrence of micropollutants. The potential for co-management of nutrients and organic micropollutants exists, but few studies have comprehensively evaluated the suite of contaminants associated with different water quality management practices (riparian zone restoration, stormwater management, etc.). Furthermore, most studies dealing with the determination of micropollutants in environmental samples include only a limited number of target analytes, leaving many contaminants undetected. To address this limitation, there has been a gradual shift in environmental monitoring from using target analysis to either suspect screening analysis (SSA) or non-targeted analysis (NTA), which relies on accurate mass measurements, mass spectral fragmentation patterns, and retention time information obtained using liquid chromatography coupled to high-resolution mass spectrometry. The work presented in this paper focuses on a wide-scope detection of micropollutants in surface water samples from the Potomac River watershed (United States). An in-house database composed of 1039 compounds based on experimental analysis of primary standards was established, and SSA workflow was optimized and applied to determine the presence of micropollutants in surface water. A total of 103 micropollutants were detected in the samples, some of which are contaminants that were not previously monitored and belong to various classes such as pharmaceuticals, personal care products, per-and polyfluoroalkyl substances and other persistent industrial chemicals. The impact of best management practices being implemented for nitrogen and phosphorus reductions were also assessed for their potential to reduce micropollutant transport. This work illustrates the advantages of suspect screening methods to determine a large number of micropollutants in environmental samples and reveals the potential to co-manage a diverse array of micropollutants based on shared transport and transformation mechanisms in watersheds.

Perfluoroalkyl and polyfluoroalkyl substances (PFASs) are used in various fields but raise concerns regarding human health and environmental consequences. Among PFASs, perfluorooctanoic acid (PFOA) and short-chain perfluoroalkyl carboxylic acids (SC PFCAs) are detectable in skin-contact consumer products and have dermal absorption potential. Here, we investigated the effects of dermal exposure to PFOA and SC PFCAs using in vitro and in vivo models. Human skin equivalents were topically treated with 0.25 mM and 2.5 mM PFOA and SC PFCAs (perfluoropentanoic acid, PFPeA; perfluorohexanoic acid, PFHxA; and perfluoroheptanoic acid, PFHpA) for 6 days, and cell viability, interleukin (IL)-1α, oxidative stress markers (malondialdehyde, MDA; and 8-hydroxydeoxyguanosine, 8-OHdG), and histopathology were examined. MDA levels were significantly higher in the PFASs groups than in controls. Compared with SC PFCAs, 2.5 mM PFOA caused more IL-1α (p < 0.001) release, decreased skin thickness and microscopic abnormalities. To evaluate systemic effects, Sprague Dawley (SD) rats were dermally treated with 250 and 1000 mg/kg PFHpA for 2 weeks and clinical and anatomic pathology were assessed. At 1000 mg/kg, 83% of the rats died, with severe ulcerative dermatitis at the application site. Adverse PFHpA-treated systemic changes were observed in the kidney, liver and testes, and histopathologic lesions such as renal tubular necrosis, hepatocellular necrosis, and germ cell degeneration were seen at 250 and 1000 mg/kg. Our study suggests that SC PFCAs have fewer effects on the skin than PFOA, but SC PFCAs can have adverse effects on major organs with systemic exposure at high concentrations. [ABSTRACT FROM AUTHOR]

Background: Puerto Rico has over 200 hazardous waste sites, as well as higher rates of several adverse health outcomes compared to the mainland US. In response to concerns of potential links between environmental contaminant exposure and preterm birth, the Puerto Rico Testsite for Exploring Contamination Threats (PROTECT) birth cohort was formed. Biomarkers of exposure for several contaminants have been found to be elevated in this cohort compared to women of child-bearing age in the National Health and Nutrition Examination Survey (NHANES). However, exposure to persistent organic pollutants (POPs) has not been evaluated.Methods: In this preliminary analysis, we measured four classes of POPs, including perfluoroalkyl substances (PFASs), polybrominated diphenyl ethers (PBDEs), polychlorinated biphenyls (PCBs), and several persistent pesticides in serum samples collected at 16-20 weeks gestation from the first 48 women enrolled in PROTECT. We performed descriptive analyses for each analyte, assessed correlations between analytes using Spearman correlations, and compared serum levels in PROTECT women to levels in NHANES women aged 18-40.Results: Several PFASs were detected in 96-100% of samples, with moderate to strong correlations between most PFASs (range r = 0.44-0.88). BDE47, BDE153, PCB 138-158, PCB153 and p,p'-dichlorodiphenyldichloroethene (p,p'-DDE) were detected in the majority of samples, with strong correlations between PCBs and p,p'-DDE (range r = 0.59-0.74). The median concentration for each analyte was lower than, sometimes by a factor of 4 (e.g. BDE47, p,p'-DDE), the median concentration reported in NHANES women aged 18-40.Conclusion: Although participants in the ongoing PROTECT birth cohort have higher exposure to many environmental contaminants, this preliminary study suggests that they have lower exposure to several POPS, specifically PCBs, OCPs, PFASs, and PBDEs. [ABSTRACT FROM AUTHOR]

Abstract 6:2 fluorotelomer sulfonamidoalkyl betaine (6:2 FTAB) is a major component of aqueous film-forming foams (AFFFs) used for firefighting and is frequently detected, along with one of its suspected transformation products, 6:2 fluorotelomer sulfonate (6:2 FTSA), in terrestrial and aquatic ecosystems impacted by AFFF usage. Biochemical processes underlying bacterial biodegradation of these compounds remain poorly understood due to a lack of pure culture studies. Here, we characterized the water-soluble and volatile breakdown products of 6:2 FTSA and 6:2 FTAB produced using Gordonia sp. strain NB4-1Y cultures over seven days under sulfur-limited conditions. After 168 h, 99.9% of 60 μM 6:2 FTSA was degraded into ten major breakdown products, with a mol% recovery of 88.2, while 70.4% of 60 μM 6:2 FTAB was degraded into ten major breakdown products, with a mol% recovery of 84.7. NB4-1Y uses two pathways for 6:2 FTSA metabolism, with 55 mol% of breakdown products assigned to a major pathway and <1.0 mol% assigned to a minor pathway. This work indicates that rapid transformation of 6:2 FTSA and 6:2 FTAB can be achieved under controlled conditions and improves the bacterial metabolism of these compounds. Graphical abstract Unlabelled Image Highlights • Biotransformation of 6:2 FTAB & 6:2 FTSA was studied under sulfur-limiting conditions. • Gordonia sp. NB4-1Y rapidly metabolized 6:2 FTAB (70.4%) & 6:2 FTSA (99.9%). • 16 metabolites were identified using high resolution Orbitrap mass spectrometry. • Two distinct degradation pathways were utilized by Gordonia sp. NB4-1Y. • Major breakdown products included 6:2 FTCA, 6:2 FTUA, and 5:2 fluorotelomer ketone. [ABSTRACT FROM AUTHOR]

Abstract N-Ethyl perfluorooctane sulfonamide (EtFOSA) is a perfluorooctane sulfonate (PFOS) precursor and the active ingredient in sulfluramid, a pesticide which is used extensively in Brazil for management of leaf cutting ants. Here we investigate the occurrence of EtFOSA, PFOS, and other per- and polyfluoroalkyl substances (PFASs) in soil, eucalyptus leaves, water (ground, riverine, and coastal (estuarine/marine)) and coastal sediment from an agricultural region of Bahia State, Brazil. This area contains a larger number of eucalyptus plantations where sulfluramid is suspected to be applied. Soil, leaves, and coastal water (marine/estuarine) contained ∑PFAS concentrations of up to 5400 pg g−1, 979 pg g−1, and 1020 pg L−1, respectively, with PFAS profiles generally dominated by PFOS and perfluorooctane sulfonamide (FOSA). Coastal sediment contained ∑PFAS concentrations of up to 198 pg g−1, with PFOS, FOSA, and perfluorooctanoic acid (PFOA) being the most frequently observed PFASs. These substances are all potential EtFOSA transformation products, pointing to sulfluramid as a possible source. In riverine water, ∑PFAS concentrations of up to 8930 pg L−1 were observed. PFOS and PFOA were detected in all river water samples. Groundwater also exhibited PFAS contamination (5730 pg L−1 ∑PFASs), likely from sulfluramid use. The observation of other PFASs (e.g. perfluorobutanoic acid) in freshwater suggests that other PFAS sources (in addition to sulfluramid) may be important in this region. Overall, these data support the hypothesis that sulfluramid use contributes to the occurrence of PFASs in the Brazilian environment. Graphical abstract Image 1 Highlights • EtFOSA transformation products (e.g. PFOS, FOSA) were observed in soil, leaves, and marine waters from Brazil. • The remoteness of sampling area points to Sulfluramid use in Brazilian agriculture as a source of PFASs in this environment. • Coastal marine sediment appears to be a major sink for PFASs. [ABSTRACT FROM AUTHOR]

Aims Some persistent organic pollutants (POPs) such as hexachlorobenzene (HCB) and some polychlorinated biphenyls (PCBs) have been shown to interfere with myocardial function and geometry. We therefore investigated if also another group of POPs: per- and polyfluoroalkyl substances (PFASs) were associated with alterations in left ventricular geometry. Methods 801 subjects aged 70 years were investigated in a cross-sectional study within the scope of the Prospective Investigation of the Vasculature in Uppsala Seniors (PIVUS) study. Eight PFASs were detected in >75% of participants´ plasma by ultra-performance liquid chromatograph/tandem mass spectrometry. Left ventricular geometry was determined by echocardiography. Multivariable linear regression was used to investigate the associations between PFASs and left ventricular geometry of the heart after exclusion of subjects with previous myocardial infarction ( n  = 72). Results When adjusting for multiple comparisons, none of the eight PFASs evaluated were significantly related to left ventricular mass. However, perfluorononanoic acid (PFNA), perfluorodecanoic acid (PFDA), and perfluoroundecanoic acid (PFUnDA) were related to relative wall thickness (RWT) in a negative fashion ( p  < 0.0021). Besides being inversely related to RWT, PFNA was also positively related to left ventricular end-diastolic volume (LVEDD) ( p  < 0.0021). These analyses were adjusted for traditional cardiovascular risk factors. Conclusion In this cross-sectional study, several of the PFASs evaluated, especially PFNA, were related to myocardial geometry: a reduction in relative wall thickness and an increase in left ventricular diameter following adjustment for traditional cardiovascular risk factors, suggesting a role for PFASs in cardiac remodeling. [ABSTRACT FROM AUTHOR]

Microplastics (MPs) in the environment are the sink and vector of organic contaminants, including per- and polyfluoroalkyl substances (PFASs). Although PFASs are low- and non-volatile compounds, they have the potential to partition and diffuse from MP into the gas phase in the environmental functions. Herein, the MP-air partition coefficient (K PA) of seven PFASs was measured using a solid-fugacity meter. The PFAS K PA values in two MPs (high-density polyethylene (HDPE) and thermoplastic polyurethane (TPU)) were determined under different times, temperatures, and relative humidities (RH), and a model was developed to predict the PFAS K PA values based on the measured data. The results showed that the K PA of PFASs increased with the prolonged partition time until 90 mins, and higher temperature and RH facilitated the distribution of PFASs in MPs into the air phase, leading to smaller K PA values. Moreover, the derived equation for predicting PFAS log K PA values was robust with 0.79 of an adjusted square of correlation coefficient (R2 adjusted = 0.79) and 0.35 of root mean squared error (RMSE = 0.35). These findings provided the first knowledge for understanding the partition behavior and fate of PFASs in the MP-air microenvironment. [Display omitted] • The K PA of seven PFASs was determined at environmentally relative conditions through a solid-fugacity meter. • The partition of PFASs between MPs and gas was equilibrium when partition time reached 90 mins. • Increasing temperature and RH resulted in the reduction of PFAS K PA. • A derived model based on the measured data could effectively predict the K PA of PFAS. [ABSTRACT FROM AUTHOR]

Per- and polyfluoroalkyl substances (PFASs) have been recently listed as emerging contaminants (ECs) and persistent organic pollutants (POPs) due to their human and environmental health concerns. In the last 10 years, their detection and remediation have progressed significantly. Herein, we critically review recent developments in electrochemical advanced oxidation process (EAOP) towards their remediation. Particular attentions are paid to perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA), which present the main concerns of PFASs at this time. Due to the persistence of those PFASs, other remediation approaches may experience difficulty in degrading them, whilst EAOP has demonstrated success. The fundamentals of EAOP are highlighted and the scale-up application is discussed regarding the future research directions. [ABSTRACT FROM AUTHOR]

The chemical properties of poly- and perfluoroalkyl substances (PFASs) make them widespread for use in a number of industrial and commercial products to confer water and oil-repellency characteristics and to reduce surface tension e.g. in aqueous film-forming foams (AFFFs). Some PFASs, especially perfluoroctane sulfonate, and several perfluoroalkyl carboxylic acids, are known to cause significant human and environmental negative impact. Our knowledge on the content of PFASs in products remains scarce due to limited information available, thus impeding any precise assessment of human exposure and environmental release upon use. This study aimed at analyzing a wide variety of liquid products (n = 194) likely to contain PFASs, including impregnating agents, lubricants, cleansers, polishes, AFFFs and other industrial products. By means of LC- and GC-MS/MS analytical techniques, 24 PFASs (from 41 targeted PFASs) were detected and quantified in 55% of samples. PFAS quantification and profiling was found to be consumer product specific. PFASs were mostly detected in AFFF (90%) and impregnating agents (60%) with mainly ionic and neutral species, respectively. In particular, the fluorotelomer alcohols 6:2, 8:2 and 10:2 FTOHs were detected in 40–50% of impregnating agents. Further investigation by Fast Atom Bombardment Mass Spectrometry (FAB-MS) on a set of AFFF samples allowed the characterization of 8 different PFAS classes as major components in these formulations. Results demonstrated that numerous and diversified PFAS are currently used in specific commercial products, implying significant human exposure and environmental release that necessitate further research concerning their toxicological impact. [ABSTRACT FROM AUTHOR]

2,3,3,3-Tetrafluoro-2-(heptafluoropropoxy)-propanoate was designed to replace perfluorooctanoic acid (PFOA), which has been mostly phased out of U.S. production due to environmental persistence, detectable human, and wildlife serum concentrations, and reports of systemic toxicity. In rodent models, PFOA exposure suppresses T cell-dependent antibody responses (TDAR) and vaccine responses in exposed humans. To determine replacement compound effects on TDAR and related parameters, male and female C57BL/6 mice were gavaged with 0, 1, 10, or 100 mg/kg/day for 28 days. Mice immunized with antigen on day 24 were evaluated for TDAR and splenic lymphocyte subpopulations 5 days later. Serum and urine were collected for test compound concentrations and liver peroxisome proliferation was measured. Relative liver weight at 10 and 100 mg/kg and peroxisome proliferation at 100 mg/kg were increased in both sexes. TDAR was suppressed in females at 100 mg/kg. T lymphocyte numbers were increased in males at 100 mg/kg; B lymphocyte numbers were unchanged in both sexes. Females had less serum accumulation and higher clearance than males, and males had higher urine concentrations than females at all times and doses. While this PFOA-replacement compound appears less potent at suppressing TDAR relative to PFOA, it produces detectable changes in parameters affected by PFOA; further studies are necessary to determine its full immunomodulatory profile and potential synergism with other per- and polyfluoroalkyl substances of environmental concern. [ABSTRACT FROM AUTHOR]

Perfluoroalkyl and polyfluoroalkyl substances (PFASs) are emerging contaminants that have been detected in the environment, biota, and humans. Drinking water is a route of exposure for populations consuming water contaminated by PFAS discharges. This research study reports environmental measurement concentrations, mass flows, and the fate of dozens of PFASs in a river receiving effluents from two fluoropolymer manufacturing facilities. In addition to quantified levels of PFASs using LC- and GC-MS analytical methods, the total amount of unidentified PFASs and precursors was assessed using two complementary analytical methods, absorbable organic fluorine (AOF) determination and oxidative conversion of perfluoroalkyl carboxylic acid (PFCA) precursors. Several dozen samples were collected in the river (water and sediment) during four sampling campaigns. In addition, samples were collected in two well fields and from the outlet of the drinking water treatment plants after chlorination. We estimated that 4295 kg PFHxA, 1487 kg 6:2FTSA, 965 kg PFNA, 307 kg PFUnDA, and 14 kg PFOA were discharged in the river by the two facilities in 2013. High concentrations (up to 176 ng/g dw) of odd long-chain PFASs (PFUnDA and PFTrDA) were found in sediment samples. PFASs were detected in all 15 wells, with concentrations varying based on the location of the well in the field. Additionally, the presence of previously discharged PFASs was still measurable. Significant discrepancies between PFAS concentration profiles in the wells and in the river suggest an accumulation and transformation of PFCA precursors in the aquifer. Chlorination had no removal efficiency and no unidentified PFASs were detected in the treated water with either complementary analytical method. Although the total PFAS concentrations were high in the treated water, ranging from 86 to 169 ng/L, they did not exceed the currently available guideline values. [ABSTRACT FROM AUTHOR]

Per- and polyfluoroalkyl substances (PFASs) were one of the priority substance groups selected which have been investigated under the ambitious European Joint programme HBM4EU (2017–2022). In order to answer policy relevant questions concerning exposure and health effects of PFASs in Europe several activities were developed under HBM4EU namely i) synthesis of HBM data generated in Europe prior to HBM4EU by developing new platforms, ii) development of a Quality Assurance/Quality Control Program covering 12 biomarkers of PFASs, iii) aligned and harmonized human biomonitoring studies of PFASs. In addition, some cohort studies (on mother-child exposure, occupational exposure to hexavalent chromium) were initiated, and literature researches on risk assessment of mixtures of PFAS, health effects and effect biomarkers were performed. The HBM4EU Aligned Studies have generated internal exposure reference levels for 12 PFASs in 1957 European teenagers aged 12–18 years. The results showed that serum levels of 14.3% of the teenagers exceeded 6.9 μg/L PFASs, which corresponds to the EFSA guideline value for a tolerable weekly intake (TWI) of 4.4 ng/kg for some of the investigated PFASs (PFOA, PFOS, PFNA and PFHxS). In Northern and Western Europe, 24% of teenagers exceeded this level. The most relevant sources of exposure identified were drinking water and some foods (fish, eggs, offal and locally produced foods). HBM4EU occupational studies also revealed very high levels of PFASs exposure in workers (P95: 192 μg/L in chrome plating facilities), highlighting the importance of monitoring PFASs exposure in specific workplaces. In addition, environmental contaminated hotspots causing high exposure to the population were identified. In conclusion, the frequent and high PFASs exposure evidenced by HBM4EU strongly suggests the need to take all possible measures to prevent further contamination of the European population, in addition to adopting remediation measures in hotspot areas, to protect human health and the environment. HBM4EU findings also support the restriction of the whole group of PFASs. Further, research and definition for additional toxicological dose-effect relationship values for more PFASs compounds is needed. [Display omitted] • Serum PFASs levels were measured in 1957 European teenagers. • A quarter of the teenagers had levels above which health effects cannot be excluded. • Some European workers also had very high serum PFASs levels. • The population living in hotspots has a higher exposure and risk of disease. [ABSTRACT FROM AUTHOR]

Per- and polyfluoroalkyl substances (PFASs) present adverse effects for human health, which result in strong needs for reliable tools monitoring personal exposure to PFASs. This study manufactured two wristbands of high density polyethylene (HDPE) and thermoplastic polyurethane (TPU), and used the wristbands to monitor PFASs personal exposure. The analytical method was developed to measure 32 PFASs in the paired HDPE and TPU wristbands worn by 60 postgraduates. Twenty-nine of 32 PFASs were detected and hexafluoropropylene oxide dimer acid (HFPO-DA) was predominant individual PFASs with median concentrations of 337 and 554 pg/g for HDPE and TPU wristbands respectively. The gender and grade of students had moderate effects on PFASs distribution in the wristbands. Higher PFASs levels were determined in the two wristbands worn by the male students compared to the females, and the greatest PFASs concentration was observed in the wristbands worn by the first-year postgraduates, follow by second- and third-year postgraduates. Additionally, significant correlations between paired HDPE and TPU wristbands were observed for perfluorobutanoic acid (PFBA), perfluorohexane sulfonic acid (PFHxS), perfluoroheptane sulfonic acid (PFHpS), perfluorooctane sulfonic acid (PFOS), and HFPO-DA. These results suggest that HDPE and TPU wristbands can be used as effective tools for monitoring personal PFAS exposure. [Display omitted] • HFPO-DA was predominant individual PFAS in HDPE and TPU wristbands worn by postgraduates. • Gender and grade of students had effects on PFASs distribution in wristbands. • The as-made HDPE and TPU wristbands are portable, inexpensive, and noninvasive. • The wristbands have the potential to become passive samplers evaluating PFASs personal exposure. [ABSTRACT FROM AUTHOR]

The aim of this critical review is i) to summarize the occurrence of Per- and polyfluoroalkyl substances (PFASs) in landfills; ii) to outline the environmental fate and transport of PFASs in landfills; iii) to compare the treatment technologies of PFASs in landfill leachate and remediation methods of PFASs in surrounding groundwater; iv) to identify the research gaps and suggest future research directions. In recent years, PFASs have been detected in landfills around the world, among which Perfluoroalkyl acids (PFAAs) especially Perfluorooctanoic acid (PFOA) and Perfluorooctane sulfonic acid (PFOS) are mostly studied due to their long-term stability. Short-chain PFASs (<8 carbons) are more common than long-chain PFASs (≧8 carbons) in landfill leachate. PFASs in landfill leachate are eventually transported to the surrounding groundwater, surface water and soil. Some PFASs evaporate from landfills to the ambient air. To avoid the environmental and health risks of PFASs in landfills, new technologies and combined use of existing technologies have been implemented to treat PFASs in landfill leachate. Integrated remediation methods are applied to control the diffusion of PFASs in groundwater surrounding landfills. In future, the mechanisms of PFAAs precursors degradation, the correlation among PFASs in different environmental media around landfills, as well as the environmental behavior and toxic effect of combined pollutants together with PFASs in landfill leachate and surrounding groundwater should be studied. [ABSTRACT FROM AUTHOR]

The impact of point and diffuse sources for 26 per- and polyfluoroalkyl substances (PFASs) in northern Europe were investigated by studying Swedish rivers ( n = 40) and recipient seawater (Baltic Sea and Kattegat; n = 18). Different composition profiles were observed in the rivers, with ten rivers having a remarkably high fraction of perfluoroalkane sulfonic acids (PFSAs; 65% of the ƩPFASs) as compared to other rivers (19%) suggesting major impact of one or several source types dominated by PFSAs. Population density and low latitude (south) were strongly correlated to the widely used perfluorooctanesulfonic acid (PFOS), perfluorooctanoic acid (PFOA) as well as to perfluorohexanesulfonic acid (PFHxS). Significant relationships between several PFCAs and PFSAs (i.e. perfluorobutanoic acid (PFBA), perfluoroheptanoic acid (PFHpA), PFOA, perfluorobutanesulfonic acid (PFBS), and PFHxS) and dissolved organic carbon (DOC) were detected ( p < 0.05), indicating chemical binding and co-transport with DOC in fresh water and seawater. Partial least squares regression analysis showed that perfluoroalkyl carboxylic acids (PFCAs) were related to latitude according to their perfluorocarbon chain length (C 3 , C 7 , C 8 , C 9 , C 10 and C 11 ), with longer chains associated with higher latitudes. This suggests the presence of mechanisms promoting higher prevalence of longer chained PFCAs in the north, e.g. precursor degradation, and/or aerosol associated stabilization of PFCAs and their precursors. [ABSTRACT FROM AUTHOR]

Lake Tana is Ethiopia's largest lake and there are plans to increase the harvest of fish from the lake. The objective of this study was to assess the levels of poly- and perfluoroalkyl substances (PFASs) in different compartments of the lake (water, sediment, and fish muscle tissue), and its implications for human exposure. The results showed higher PFAS concentrations in piscivorous fish species ( Labeobarbus megastoma and Labeobarbus gorguari ) than non-piscivorous species ( Labeobarbus intermedius, Oreochromis niloticus and Clarias gariepinus ) and also spatial distribution similarities. The ∑PFAS concentrations ranged from 0.073 to 5.6 ng L −1 (on average, 2.9 ng L −1 ) in surface water, 0.22–0.55 ng g −1 dry weight (dw) (on average, 0.30 ng g −1 dw) in surface sediment, and non-detected to 5.8 ng g −1 wet weight (ww) (on average, 1.2 ng g −1 ww) in all fish species. The relative risk (RR) indicates that the consumption of fish contaminated with perfluorooctane sulfonate (PFOS) will likely not cause any harmful effects for the Ethiopian fish eating population. However, mixture toxicity of the sum of PFASs, individual fish consumption patterns and increasing fish consumption are important factors to consider in future risk assessments. [ABSTRACT FROM AUTHOR]

A wide range of per- and polyfluoroalkyl substances (PFASs), including fluorotelomer alcohols (FTOHs), perfluorooctane sulfonamidoethanols (FOSEs), perfluoroalkyl carboxylic acids (PFCAs), and perfluoroalkane sulfonic acids (PFSAs), were measured in fifteen house dust and two nonresidential indoor dust of Korea. Total concentrations of PFASs in house dust ranged from 29.9 to 97.6 ng g − 1 , with a dominance of perfluorooctane sulfonic acid (PFOS), followed by 8:2 FTOH, N -Ethyl perfluorooctane sulfonamidoethanol (EtFOSE), perfluoroctanoic acid (PFOA). In a typical exposure scenario, the estimated daily intakes (EDIs) of total PFASs via house dust ingestion were 2.83 ng d − 1 for toddlers and 1.13 ng d − 1 for adults, which were within the range of the mean EDIs reported from several countries. For PFOA and PFOS exposure via house dust ingestion, indirect exposure (via precursors) was a minor contributor, accounting for 5% and 12%, respectively. An aggregated exposure (hereafter, overall-EDIs) of PFOA and PFOS occurring via all pathways, estimated using data compiled from the literature, were 53.6 and 14.8 ng d − 1 for toddlers, and 20.5 and 40.6 ng d − 1 for adults, respectively, in a typical scenario. These overall-EDIs corresponded to 82% (PFOA) and 92% (PFOS) of a pharmacokinetic model-based EDIs estimated from adults' serum data. Direct dietary exposure was a major contributor (> 89% of overall-EDI) to PFOS in both toddlers and adults, and PFOA in toddlers. As for PFOA exposure of adults, however direct exposure via tap water drinking (37%) and indirect exposure via inhalation (22%) were as important as direct dietary exposure (41%). House dust-ingested exposure (direct + indirect) was responsible for 5% (PFOS in toddlers) and < 1% (PFOS in adults, and PFOA in both toddlers and adults) of the overall-EDIs. In conclusion, house-dust ingestion was a minor contributor in this study, but should not be ignored for toddlers' PFOS exposure due to its significance in the worst-case scenario. [ABSTRACT FROM AUTHOR]

As a class of common emerging pollutants, per- and polyfluoroalkyl substances (PFASs) and their alternatives have been widely detected in various environmental matrices, exhibiting a great threat to the ecological environment and human health. Nevertheless, changes in biomolecular structure and function of duckweed caused by PFASs and their alternatives remain unknown thus far. Herein, the effects of four PFASs, including two common legacy PFASs (perfluorooctane sulfonic acid (PFOS) and perfluorooctanoic acid (PFOA)) and two PFASs alternatives (perfluorobutane sulfonic acid (PFBS) and 1H,1H,2H, 2H-perfluorooctane sulfonic acid (6:2 FTS)) on duckweed (Lemna minor) at biochemical level were investigated with Fourier transform infrared spectroscopy (FTIR). Although no obvious inhibitions were observed in the growth of L. minor with PFASs exposure at three levels of 1 μg L−1, 100 μg L−1, and 10 mg L−1, significant structural and functional alterations were induced at the biochemical level. In response to PFASs exposure, lipid peroxidation, proteins aggregation and α-helix to β-sheet transformation of the protein conformation, as well as changes of DNA conformations were detected. Moreover, alterations in lipid, protein, and DNA were proved to be concentration-related and compound-specific. Compared to the two legacy PFASs (PFOS and PFOA), alternative ones exhibited greater effects on the biological macromolecules of L. minor. The findings of this study firstly reveal structural and functional alterations in L. minor induced by PFASs exposure, providing further understanding of their toxicity effects. [Display omitted] • Toxic effects of PFOS, PFOA, PFBS and 6:2 FTS on duckweeds were assessed by FTIR. • PFASs caused concentration-related and compound-specific toxic effects on duckweeds. • PFBS and 6:2FTS exhibited more obvious impacts on duckweeds than PFOS and PFOA. • Changes in the structure and composition of biomolecules were observed. • PFASs induced lipid peroxidation, protein aggregation and DNA conformation. [ABSTRACT FROM AUTHOR]

Data on the occurrences of legacy and alternative per- and polyfluoroalkyl substances (PFASs) in glioma are scarce. It remains unclear if PFASs exposure is related to the prevalence of glioma. A total of 137 glioma and 40 non-glioma brain tissue samples from patients recruited from the Nanfang Hospital, South China were analyzed for 17 PFAS compounds. Perfluorohexanoic acid, perfluorooctanoic acid (PFOA), perfluorooctane sulfonate (PFOS), perfluorooctane sulfonamide (FOSA), and 6:2 chlorinated polyfluorinated ether sulfonate were frequently detected (> 60 %) in glioma. The total concentrations (range; median) of 17 PFASs in glioma (0.20–140; 3.1 ng g−1) were slightly higher than those in non-glioma (0.35–32; 2.2 ng g−1), but without statistical significance. The PFAS concentrations in males were statistically higher (p < 0.05) than those in females. Elevated glioma grades were associated with higher concentrations of PFOA, PFOS, and FOSA. Positive correlations were observed between PFAS concentrations (especially for PFOA) and Ki-67 or P53 expression, pathological molecular markers of glioma. Our findings suggested that exposure to PFASs might increase the probability to develop glioma. This is the first case study demonstrating associations between PFASs exposure and brain cancer. More evidences and potential pathogenic mechanisms warranted further investigations. [Display omitted] • PFASs were commonly detected in glioma and non-glioma human brain tissues. • PFOA concentration positively correlated with glioma grade, Ki-67 and P53 expression. • Glioma is partially ascribed to exposure to legacy and alternative PFASs. [ABSTRACT FROM AUTHOR]

The transport behavior of perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) in porous media controls their final environmental fate and risk. Current work used column experiments and a two-site kinetic retention model to reveal the effect of dissolved organic matter (DOM) on their migration under the interference of solution ionic strength and cation type. Results showed the migration of PFOA and PFOS was inhibited by 1 mg/L DOM but promoted by 10 mg/L DOM. Compared with the presence of 1 mg/L DOM, increasing ionic strength (1–10 mM) and changing cation type (Na+ to Ca2+) had a stronger inhibitory effect on PFOS transport. However, the opposite is true for PFOA due to its different molecular structure and adsorption behavior from PFOS. Ca2+ was more tended to interact with PFOS (owing to its sulfonate group) than with PFOA, and PFOS had a higher affinity with Ca2+ than with DOM. Cation bridging and increasing ionic strength could promote the inhibition of PFOA and PFOS transport by 1 mg/L DOM. The influence of DOM on PFASs transport could be very complex in the natural environment due to the variable solution chemistry conditions of pore water and physico-chemical properties of PFASs. The results of this work have scientific significance for better evaluating and managing the environmental risk of soil PFASs. [Display omitted] • DOM-mediated transport of PFOA and PFOS is solution chemistry dependent. • PFOA and PFOS transport was inhibited by deposited DOM but facilitated by free DOM. • The DOM-affected transport of PFOA was less disturbed by ionic strength and Ca2+. • PFOS preferred to interact with Ca2+ rather than DOM. • Ca2+ and higher ionic strength promoted the effect of deposited DOM. [ABSTRACT FROM AUTHOR]

The levels of legacy per- and polyfluoroalkyl substances (PFASs) have been growing in the environmental matrices and blood of residents living around the fluorochemical industrial park (FIP) in Fuxin of China over the past decade. Although some recent studies have reported occurrence of novel PFAS alternatives in biotic and abiotic matrices near fluorochemical facilities worldwide, little is known about novel PFAS congeners in maternal sera, umbilical cord sera, and placentas from the female residents close to the FIP and their related health risks. In this study, 50 paired samples of maternal and cord serum as well as placenta were derived from Fuxin pregnant women at delivery, and 21 target analytes of legacy PFASs in all the samples were analyzed via high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS), revealing that PFBS, PFBA, and PFOA were the dominant PFAS contaminants observed in the whole samples. Based upon the suspect screening through high-resolution mass spectrometry (HRMS), 49 novel PFASs assigned to 11 classes were further identified in the Fuxin samples, of which, 20 novel congeners in 4 classes were reported in human blood and placentas for the first time. Moreover, the coefficients for mother-placenta transfer (R m/p), placenta-newborn transfer (R p/n), and mother-newborn transfer (R m/n) of legacy PFASs could be calculated with median values of 1.7, 1.1, and 2.0, respectively, and R m/p , R p/n , and R m/n for each novel PFAS identified were also estimated with the median values of 0.9, 1.2, and 0.8 individually. Accordingly, novel PFASs contributed 90% of all the legacy and novel PFASs in maternal sera and even occupied 96% of the whole PFASs in both placentas and cord sera. In addition, significant associations were determined among the neonate birth outcomes and serum concentrations of thyroid hormone, sex hormone, and glucocorticoid, together with the levels of certain legacy and novel PFASs in cord sera. [Display omitted] • PFBS, PFBA, and PFOA were the dominant legacy PFASs in Fuxin paired human samples. • 49 novel PFASs in 11 classes were identified in human samples via suspect screening. • 20 novel PFASs from 4 classes were reported in human samples for the first time. • Novel PFASs contributed over 90% of all the legacy and novel PFASs in human samples. • Novel PFASs in cord sera could affect neonate birth outcomes and hormone levels. [ABSTRACT FROM AUTHOR]

ABSTRACT This review examines the usefulness of the metrics BMF (biomagnification factor) and TMF (trophic magnification factor), derived from field measurements of the levels of contaminants in naturally occurring biota, for characterizing the bioaccumulation potential ('B') of chemicals. Trophic magnification factor and BMF values greater than 1.0 are often considered to be the most conclusive indicators of B status, and the TMF criterion has been referred to as the 'gold standard' for B categorization. Although not wishing to dispute the theoretical primacy of field-derived BMFs and TMFs as B metrics, we make the case that, in practice, the study-to-study (and even within-study) variability of the results is so great that they are of very restricted usefulness for assessing B status, at least in the case of the per- and polyfluoroalkyl substances (PFASs), on which we focus here. This conclusion is based on an analysis of the results of 24 peer-reviewed studies reporting field-derived BMFs or TMFs for 14 PFASs, for which BMF values often range over several orders of magnitude from <<1.0 to >>1.0, sometimes even in the same study. For TMFs, the range is a factor of approximately 20 for the most intensely studied PFASs (perfluorooctanoic acid [PFOA] and perfluorooctanesulfonic acid [PFOS]). We analyze the possible causes for such variability: To some extent it results from the differing ways in which the metrics are expressed, but most of the scatter is likely attributable to such factors as nonachievement of the tacitly assumed steady-state conditions, uncertainties in the feeding ecology, the impact of metabolism of precursor compounds, and so forth. As more trustworthy alternatives to field-derived BMFs and TMFs, we suggest the implementation of dietary BMF studies performed under strictly controlled conditions on aquatic, terrestrial, and avian species, as well as the consideration of measured elimination half-lives, which have been demonstrated to be directly related to BMF values. Integr Environ Assess Manag 2016;12:6-20. © 2015 The Author. Integrated Environmental Assessment and Management published by Wiley Periodicals, Inc. on behalf of SETAC. [ABSTRACT FROM AUTHOR]

The spatial trend of perfluoroalkyl substances (PFASs) along Xiaoqing River and its tributaries was studied to characterize isomer profiles and quantify emissions from fluoropolymer (FP) manufacturers in China. Substantially elevated ∑PFAS concentrations downstream of tributary 4 demonstrated that the emissions from this FP manufacturer dominated total riverine discharges. Isomer profiles of perfluorooctanoic acid (PFOA) in water displayed a stepwise increase in percentage branched PFOA downstream of tributary 3 (14.0%) and 4 (22.7%) reflecting the importance of FP sources. Strong positive correlations between PFOA isomers in water downstream of tributary 4 indicated that isomer profiles were conserved from emission sources to the final reservoir. Riverine discharges of PFOA (23–67 t/yr) were in agreement with theoretical emission calculations from FP production (68 t/yr) whereas large discrepancies between the two methodologies were observed for perfluorobutanoic acid and perfluoropentanoic acid. Collectively, this study fills critical knowledge gaps for understanding ongoing global sources of PFASs. [ABSTRACT FROM AUTHOR]

Fire training facilities are potential sources of per- and polyfluoroalkyl substances (PFASs) to the nearby environment due to the usage of PFAS-containing aqueous fire-fighting foams (AFFFs). The multimedia distribution of perfluoroalkyl carboxylates (PFCAs), perfluoroalkyl sulfonates (PFSAs), perfluorooctanesulfonamide (PFOSA) and 6:2 fluorotelomer sulfonate (FTSA) was investigated near a fire training facility at Stockholm Arlanda Airport in Sweden. The whole body burden of PFASs in European perch ( Perca fluviatilis ) was 334 ± 80 μg absolute and was distributed as follows: Gonad > liver ≈ muscle > blood > gill. The bioconcentration factor (BCF) and sediment/water partition coefficient ( K d ) increased by 0.6–1.7 and 0.2–0.5 log units, respectively, for each additional CF 2 moiety for PFCAs and PFSAs. PFAS concentrations in water showed no significant decreasing trend between 2009 and 2013 ( p > 0.05), which indicates that Stockholm Arlanda Airport may be an important source for long-term contamination of the nearby environment with PFASs. [ABSTRACT FROM AUTHOR]