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Background: Total knee arthroplasty (TKA) for poliomyelitis patients is controversial due to its higher complication rate compared with that of TKA for osteoarthritis. In this study we evaluated the results, at a minimum of 10 years, of a consecutive series of third-generation rotating hinge total knee arthroplasties (RHKs) in patients affected by poliomyelitis. Methods: We conducted a retrospective analysis of 14 patients (15 TKA) performed between January 2000 and October 2021 that underwent a third-generation rotating hinge arthroplasty (Nex Gen RHK Zimmer). The clinical results using the American Knee Society Score (AKSS) were evaluated at an average follow-up of 154 months (min 120–max 216 months). Result: Of the 15 cases, 2 were lost at follow-up and 1 was excluded by the final evaluation due to infection. Of the remaining 12 cases at the final follow-up, the average AKKS clinical score improved from 30.08 points preoperatively, to 78.83 points postoperatively, while the average AKKS function score improved from 29.17 points to 56.25 points. We report only one intraoperative complication consisting of a femoral fracture at the tip of the stem, and a case of patellar fracture occurred 3 months after the index procedure. The presence of radiolucent lines was sporadic, while radiosclerotic lines were commonly visible around the stem extension, predominantly at the tip. Conclusions: The third generation of rotating hinge knee replacement has proved to be effective in polio patients, improving clinical and functional scores, and no cases of mechanical failure occurred.

Abstract Effective site characterization in highly urbanized coastal lowlands requires accurate stratigraphic and geophysical investigations. In these regions, which typically host shallowly buried paleovalley systems formed in response to Quaternary glacio‐eustatic fluctuations, the marked lithologic contrast between soft sediment paleovalley fills and the adjacent, stiff substrate has the potential to modify earthquake motions, and assessment of critical parameters, such as shear wave velocities (VS) and resonance frequencies (f), should be coupled with detailed stratigraphic architecture. To evaluate the potential of the microtremor horizontal‐to‐vertical spectral ratio (mHVSR) for paleovalley recognition and mapping, we performed mHVSR measurements along the Adriatic coastal plain of Italy, where two paleovalley systems (Pescara and Manfredonia) have been recently identified. In both areas, we detected rapid lateral variations in resonance frequencies and highlighted laterally continuous impedance contrasts. Relying on a robust stratigraphic framework, we carefully evaluated the relation between geological and geophysical data and identified the stratigraphic surfaces responsible for the observed resonances. We derived VS models for the sediment fill, reconstructing the geometry of the two buried paleovalleys. We address the importance of evaluating the geological context when designing microzonation studies, for a reliable interpretation of changes in resonance frequencies.

Numerical models have become a useful tool for predicting the potential risk caused by debris flows. Although a variety of numerical models have been proposed for the runout simulation of debris flows, the performances of these models in simulating specific events generally vary due to the difference in solving methods and the simulation of the entrainment/deposition processes. In this paper, two typical depth-averaged models have been used to analyze a well-documented debris-flow event that occurred in the Cancia basin on 23 July 2015. The simulations with and without bed entrainment are conducted to investigate the influence of this process on the runout behavior of the debris flow. Results show that the actual runout can be reproduced only by considering bed entrainment. If basal erosion is not taken into account, part of the debris mass deviates from the main path and both models predict unrealistic bank overflows not observed in the field. Moreover, the comparison between measured and simulated inundated areas shows that both models perform generally well in the terms of simulating the erosion-deposition pattern, although the DAN3D model predicts a greater lateral spreading and a thinner depositional thickness compared to Shen’s model. A simple numerical experiment obtains similar consequences and further illustrates the possible reasons that cause these differences.

Replication stress has been associated with transient remodelling of replication intermediates into reversed forks, followed by efficient fork restart. Here the authors systematically analyse the role of RAD51 paralogs in these transactions, providing insights on the mechanistic role of different complexes of these proteins.

The triggering threshold is one of the most important parameters for landslide early warning systems (EWSs) at the slope scale. In the present work, a velocity threshold is recommended for an early warning system of the Gapa landslide in Southwest China, which was reactivated by the impoundment of a large reservoir behind Jinping’s first dam. Based on GNSS monitoring data over the last five years, the velocity threshold is defined by a novel method, which is implemented by the forward and reverse double moving average of time series. As the landslide deformation is strongly related to the fluctuations in reservoir water levels, a crucial water level is also defined to reduce false warnings from the velocity threshold alone. In recognition of the importance of geological evolution, the evolution process of the Gapa landslide from topping to sliding is described in this study to help to understand its behavior and predict its potential trends. Moreover, based on the improved Saito’s three-stage deformation model, the warning level is set as “attention level”, because the current deformation stage of the landslide is considered to be between the initial and constant stages. At present, the early warning system mainly consists of six surface displacement monitoring sites and one water level observation site. If the daily recorded velocity in each monitoring site exceeds 4 mm/d and, meanwhile, the water level is below 1820 m above sea level (asl), a warning of likely landslide deformation accelerations will be released by relevant monitoring sites. The thresholds are always discretely exceeded on about 3% of annual monitoring days, and they are most frequently exceeded in June (especially in mid-June). The thresholds provide an efficient and effective way for judging accelerations of this landslide and are verified by the current application. The work presented provides critical insights into the development of early warning systems for reservoir-induced large-scale landslides.

FANCD2-associated nuclease 1 (FAN1) is a key protein involved in the metabolism of DNA and in human pathologies. Here, the authors show that FAN1 directly interacts with PCNA at stalled replication forks to control their progression and prevent their collapse.

BRCA2 is involved in both homologous recombination (HR) and the protection of stalled replication forks from degradation. Here the authors reveal how HR factors cooperate in fork remodeling, showing that BRCA2 supports RAD51 loading on the regressed arms of reversed replication forks to protect them from degradation.

Landslides are widespread geological features in Italy’s Northern Apennines, with slow-moving earthflows among the most common types. They develop in fine-grained rocks and are subject to periodic rainfall-induced reactivations alternating to phases of dormancy. In this paper, we use radar interferometry (InSAR) and information about landslide activity to investigate deformation signals on an areal basis and to assess the dynamics of recently reactivated earthflows. We use traditional two-pass interferometry by taking advantage of the short revisit time of the Sentinel 1 satellite to characterize 4 years of slope deformations over the 60 km2 study area, where 186 landslides are mapped. Our results show that most intense and sustained deformation signals are associated with phenomena on the verge of reactivation, indicating that radar interferometry may have a potential for early warning purposes. By focusing on three specific earthflow reactivations, we analyze their dynamics through the years that preceded their failure. Despite inherent uncertainties, it was possible to retrieve the deformation signal’s temporal evolution, which displayed seasonally recurring accelerations, peaking during the major precipitation episodes in the area.

Summary: Interstrand cross-links (ICLs) are toxic DNA lesions interfering with DNA metabolism that are induced by widely used anticancer drugs. They have long been considered absolute roadblocks for replication forks, implicating complex DNA repair processes at stalled or converging replication forks. Recent evidence challenged this view, proposing that single forks traverse ICLs by yet elusive mechanisms. Combining ICL immunolabeling and single-molecule approaches in human cells, we now show that ICL induction leads to global replication fork slowing, involving forks not directly challenged by ICLs. Active fork slowing is linked to rapid recruitment of RAD51 to replicating chromatin and to RAD51/ZRANB3-mediated fork reversal. This global modulation of fork speed and architecture requires ATR activation, promotes single-fork ICL traverse—here, directly visualized by electron microscopy—and prevents chromosomal breakage by untimely ICL processing. We propose that global fork slowing by remodeling provides more time for template repair and promotes bypass of residual lesions, limiting fork-associated processing. : Replication-coupled repair of DNA interstrand cross-links (ICLs) promotes resistance to chemotherapeutic treatments. Visualizing individual lesions and replication intermediates, Mutreja et al. report that forks slow down and reverse both at ICLs and away from lesions. This ATR-mediated response assists lesion bypass during replication and limits chromosomal breakage by fork-associated processing. Keywords: DNA replication, DNA replication stress response, replication fork reversal, fork traverse, DNA interstrand crosslinks, ATR checkpoint, global fork slowing, electron microscopy, ICL immunolabeling

The financial support for this Article was not fully acknowledged. The Acknowledgements should have included the following: This study was in part supported by the Swiss National Foundation Grant No.: 31003A-156023 to Alessandro Sartori.