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Abstract Higher usage of copper oxide nanomaterials in industrial and biomedical fields may cause an increase of these nanoparticles in aquatic environments, which could have a detrimental ecological effect. Thus, the objective of this study was to evaluate the acute toxicity of copper oxide nanoparticles on the freshwater gastropod, Biomphalaria alexandrina. Transmission electron microscopy, x-ray diffraction analysis and UV–VIS spectrophotometer of CuO NPs revealed a typical TEM image and a single crystal structure with average crystallite size of approximately 40 nm also, a sharp absorption band was appeared. Following exposure to sub-lethal concentrations of CuO NPs (LC10, 15.6 mg/l and LC25, 27.2 mg/l), treated snails revealed a significant decrease (p

Abstract Background Congenital tracheal stenosis is a rare structural obstructive disorder affecting a segment of the trachea. It is often caused by complete or nearly complete cartilaginous rings narrowing the airway. Approximately 50% of cases are associated with abnormal left pulmonary artery rings. Case presentation We present the case of a 4-month-old female infant with complex congenital tracheal stenosis associated with a left pulmonary artery vascular ring and congenital heart disease. The patient underwent successful surgical intervention involving repair of the congenital heart defect and reimplantation of the pulmonary artery, followed by sliding tracheoplasty in one surgery procedure. Postoperative outcomes were favorable, with successful extubating and resolution of respiratory symptoms. Conclusions Sliding tracheoplasty proved an effective treatment option in this complex case, highlighting its reproducibility and favorable outcomes in managing congenital tracheal stenosis.

While forest biodiversity faces a concerning decline, modern technology presents promising avenues for mitigation. However, a critical gap persists in reconciling ecological knowledge with the technical expertise required to use state-of-the-art technologies in 3D data classification. Currently, one main issue is the scarcity of 3D datasets for biodiversity, particularly within the context of machine learning applications. Unlike the straightforward classification of human-made structures, forest environments are uniquely intricate and nuanced due to its inherently complex nature. This study addresses this challenge by introducing a fully automated pipeline for tree stem 3D point cloud segmentation, focussing on a biodiversity indicator: tree-related microhabitats (TreMs). Furthermore, our research advances the field by demonstrating that machine learning models trained with labels generated by our proposed virtual reality (VR) method, Labelling Flora, yield predictions statistically similar to the traditional desktop-based labelling methods. This implies that existing 3D datasets could be augmented using the more rapid approach of VR labelling. Additionally, the findings of this paper demonstrate the potential integration of VR and immersive technology into the 3D labelling workflow, facilitating a quicker and more intuitive labelling process. This could empower users, who are non-familiar with 3D modelling, to contribute their expertise to the segmentation process.

Global navigation satellite systems have enabled significant improvements in aeronautical navigation. However, in recent years, a growing number of interference events have been reported by flight crews. In this paper, we first identify such events using crowd-sourced surveillance data collected between February and December 2022 for three different regions: the Baltic states, eastern Europe bordering the Black Sea, and the eastern Mediterranean. Then, we assess the extent and duration of these events to determine their impact on civil aviation. The analysis shows different characteristics, ranging from isolated events to regular large-scale and recurrent disruptions. Next, we identify aircraft types for the affected flights and evaluate flight plan data with respect to navigation equipment in order to identify flights that rely solely on satellite navigation and that might require assistance in the case of a loss of satellite navigation. Finally, we show the impact of radio frequency interference (RFI) on a selected passenger flight by analyzing automatic dependent surveillance-broadcast data as well as avionics data obtained from the airline’s flight data monitoring department for that specific flight and link the observations to the warnings triggered by the aircraft to alert the flight crew while encountering RFI.

ABSTRACT Among marine creatures including squids, cephalopods and Octopus, one of the most unique features is production of ink which is an extremely valuable source of pharmaceuticals. The current study investigates the ink produced from Octopus vulgaris chemically as well as its potential antioxidant, anticancer, antimicrobial, and anti-schistosomal activities in vitro. Twenty-one different compounds were identified by GC-MS analysis of Octopus vulgaris ink. Results revealed that O. vulgaris ink had antioxidant capability to cover DPPH free radical when compared to ascorbic acid. Moreover, Octopus vulgaris ink exhibited antibacterial activity against Bacillus subtilis followed by Escherichia coli and Pseudomonas aeuroginosa and exhibited a molluscicidal activity against adult Biomphalaria alexandrina snails and had a distinguished mortal effect on free larval phases of Schistosoma mansoni. Furthermore, its anticancer activity was confirmed, where IC50 value of breast cancer cell line MCF-7, human hepatocellular carcinoma (HCT) and human liver cancer cell line (HepG2) were 29.8, 38.29 and 30.38μg/mL, respectively. In conclusion, ink extracted from O. vulgaris may be considered as source of valuable compounds that can be used as molluscicidal, antioxidant, antimicrobial, anticancer and anti-schistosomal agents.

Abstract Natural products extracted from animal sources have many biological activities, such as chitosan, which is being researched for its medicinal or therapeutic potential. Syphacia muris is the most well-known intestinal nematode, infecting laboratory rats and influencing their immune systems. In this study, we looked at the anthelminthic activity of chitosan particles against S. muris infection using biochemical, immunological, and histopathological methods. Chitosan particles were characterized using Fourier-transform infrared spectroscopy (FTIR). Rats were separated into four groups, each consisting of seven individuals (n = 7). The first group was the control (non-infected), the second group was infected, and both groups received 0.5 ml of 1% glacial acetic acid orally. The third group was the infected group (treated), and the fourth group (normal) received 0.5 ml of 30 mg/kg/day chitosan dissolved in 1% glacial acetic acid for 14 days using gavage. Liver and kidney parameters, oxidative stress markers, serum levels of cytokines (IFN-γ, IL-5, IL-13, IL-33, and IL-10), as well as immunoglobulins (total IgE and IgG), were assessed. Histological examinations of host tissues (intestine, liver, kidney, and spleen) were also performed. Following chitosan treatment, a significant decrease in worm count (P

Abstract The question was asked “whether plant phytocenosis has an impact on the medical potential of the extracts from Pleurozium schreberi”. Moss samples were collected from four different phytocoenoses: mixed forest (oak-pine forest), a forest tract in pine forest, 5–15-year-old pine forest and 50-year-old pine forest. Chemical composition of the extracts, antioxidative capacity (FRAP and ABTS·+ assays), as well as biological activities including cytotoxicity for the mouse fibroblasts L929 line (MTT reduction assay), biostatic/biocidal effect against selected bacteria and fungi (broth microdilution method followed by culture on solid media), and regenerative properties on human fibroblasts HFF-1 line (scratch assay) were tested. The conducted research clearly proves that phytocenosis determines the quality of moss extracts. The analyses showed that in every examined aspect the IV-7 extract (obtained from a specimen collected in a Pinus sylvestris L. forest, monoculture up to 15 years old) exhibited the highest values and the strongest activity. Other extracts of the same species but growing in other phytocenoses—in a mixed forest (IV-5), a forest tract in a Pinus sylvestris monoculture forest (IV-6) and in a P. sylvestris forest of pine monoculture about 50 years old (IV-8) showed much weaker activity and lower values of the above-mentioned parameters. At the same time, none of the tested extracts exerted a pro-regenerative effect. The P. schreberi extracts were characterized by a varied total content of phenolic compounds in the range from 0.63 ± 0.02 to 14.01 ± 0.25 mg/g of plant material. UPLC/MS analysis showed a varied phenolic profile of the extracts, with caffeoylquinic acid and quercetin triglucoside predominating in all of them.

Terrestrial 3D reconstruction is a research topic that has recently received significant attention in the forestry sector. This practice enables the acquisition of high-quality 3D data, which can be used not only to derive physical forest criteria such as tree positions and diameters, but also more detailed analyses related to ecological parameters such as habitat availability and biomass. However, several challenges must be addressed before fully integrating this technology into forestry practices. The primary challenge is accurately georeferencing surveyed 3D data acquired in the same location and placing them into a national projection reference system. Unfortunately, due to the forest canopy, the GNSS signal is often obstructed, and it cannot guarantee sub-meter accuracy. In this paper, we have implemented an indirect georeferencing methodology based on spheres with known coordinates placed at the forest’s edge where GNSS reception was more reliable and accurate than under the canopy. We evaluated its performance through three analyses that confirmed the validity of our approach. Indeed, the accuracy of the TLS point cloud, georeferenced using our method, is within a centimetre level (4.7 cm), whereas mobile scanning methods demonstrate accuracy within the decimetre range but still less than a metre. Additionally, we have initiated the analysis of a potential future application for mixed reality headsets, which could enable real-time acquisition and visualisation of 3D data.

Abstract A muon collider would enable the big jump ahead in energy reach that is needed for a fruitful exploration of fundamental interactions. The challenges of producing muon collisions at high luminosity and 10 TeV centre of mass energy are being investigated by the recently-formed International Muon Collider Collaboration. This Review summarises the status and the recent advances on muon colliders design, physics and detector studies. The aim is to provide a global perspective of the field and to outline directions for future work.

Abstract Background In 2021, twenty out of twenty-one countries in the Eastern and Southern Africa (ESA) region introduced COVID-19 vaccines. With variable willingness to uptake vaccines across countries, the aim of the present study was to better understand factors that impact behavioral and social drivers of vaccination (BeSD). Using the theory-based “increasing vaccination model”, the drivers Thinking & Feeling, Social Processes, Motivation, and Practical Issues were adapted to the COVID-19 context and utilized in a cross-country assessment. Methods Data was collected on 27.240 health workers in Kenya, Malawi, Mozambique, South Africa and South Sudan. This was done by administering a survey of seven target questions via the UNICEF Internet of Good Things (IoGT) online platform between February and August 2021. Results Findings showed a gap between perceived importance and trust in vaccines: Most health workers thought Covid-19 vaccination was very important for their health, while less than 30% trusted it very much. The pro-vaccination social and work norm was not well established since almost 66% of all respondents would take the vaccine if recommended to them, but only 49% thought most adults would, and only 48% thought their co-workers would. Access was highlighted as a crucial barrier, with less than a quarter reporting that accessing vaccination services for themselves would be very easy. Women exhibited slightly lower scores than men across the board. When testing the associations between drivers in Kenya and South Africa, it appears that when target interventions are developed for specific age groups, social norms become the main drivers of intention to get vaccinated. Conclusions The present study revealed various key relations with demographic variables that would help immunization programmes and implementing partners to develop targeted interventions. First, there is a serious gap between perceived importance of COVID-19 vaccines and how much trust people in them. Second, problems with access are still rather serious and solving this would strongly benefit those who demand a vaccine, Third, the role of social norms is the most important predictor of willingness when considering age differences.

Abstract Background During the COVID-19 pandemic, social listening programs across digital channels have become an integral part of health preparedness and response planning, allowing to capture and address questions, information needs, and misinformation shared by users. This study identifies key social listening trends around COVID-19 vaccines in Eastern and Southern Africa and analyses how online conversations about this issue evolved over time. Methods A taxonomy developed and refined in collaboration with social and behaviour change teams was used to filter online conversations into nine subtopic categories. The taxonomy was applied to online content tracked in 21 countries in Eastern and Southern Africa over the period December 1, 2020—December 31, 2021. Metrics captured included volume of posts or articles and related user engagement. Qualitative analysis of content was conducted to identify key concerns, information voids and misinformation. Results Over 300,000 articles and posts about COVID-19 vaccines shared by users or outlets geolocated in the region were analysed. These results generated over 14 million engagements on social media and digital platforms. The analysis shows how conversations about access and availability of vaccines represented the largest share of engagement over the course of the period. Conversations about vaccine effectiveness and safety represented the second and third largest share of engagement, with peaks observed in August and November 2021. Online interest in childhood vaccination increased over time as vaccine eligibility criteria expanded in some countries in the region. Conversations mentioning mandates and certificates peaked in the last quarter of 2021, as governments as private sector entities expanded vaccine requirements. Conclusions Findings from this study show the importance of monitoring conversation trends over time and adjust social listening data collection systems to include emerging topics. The study also points to the need to consider concerns, information voids and misinformation around effectiveness and safety of vaccines in the context of overall concern for vaccine availability and access in Eastern and Southern Africa. This is fundamental to inform social and behaviour change strategies that promote vaccine demand effectively, without increasing public frustration over vaccine availability challenges and downplaying concerns around vaccine equity.

Forest digitisation is one of the next major challenges to be tackled in the forestry domain. As a consequence of tremendous advances in 3D scanning technologies, broad areas of forest can be mapped in 3D dramatically faster than 20 years ago. Consequently, capturing 3D forest point clouds with the use of 3D sensing technologies – such as lidar – is becoming predominant in the field of forestry. However, the processing of 3D point clouds to bring semantics to the 3D forestry data – e.g. by linking them with ecological values – has not seen similar advancements. Therefore, in this paper we consider a novel approach based on the use of VR (Virtual reality) as a potential solution for deriving biodiversity from 3D point clouds acquired in the field. That is, we developed a VR labelling application to visualise forest point clouds and to perform the segmentation of several biodiversity components on tree stems e.g., mosses, lichens and bark pockets. Furthermore, the VR segmented point cloud was analysed with standard accuracy and precision metrics. Namely, the proposed VR application managed to achieve an IoU (Intersection over Union) rate value of 98.74% for the segmentation of bark pockets and resp. 93.71% for the moss and lichen classes. These encouraging results reinforce the potential for the proposed VR labelling method for other purposes in the future, for example for AI (Artificial Intelligence) training dataset creation.

Monitoring biodiversity in forests is crucial for their management and preservation, especially in light of increasing climatic disturbances. However, traditional methods of surveying forest biodiversity, such as the inventory of tree-related microhabitats (TreMs), are costly and time-consuming. For many years, terrestrial laser scanning (TLS) was the main method for producing highly accurate 3D models of forests. However, with recent advancements in 3D scanning technologies, there are now numerous alternatives available on the market. The aim of this study was to evaluate the performance of four different 3D data acquisition methods, i.e. close-range photogrammetry (CRP), fish-eye photogrammetry (FEP), mobile laser scanning (MLS), and mixed reality depth camera (MRDC), in terms of accuracy and ability to measure biodiversity (TreMs) at tree-stem level, in comparison to TLS. Analysis was performed based on geometric accuracy and point neighbourhood relevance. CRP was the most accurate alternative to TLS for TreM measurement with a median error of 1.5 cm, while FEP provided a good balance between accuracy (median error 1.4 cm) and speed of data collection. Although MLS showed promising results (median error 1.6 cm), noise in the point cloud limited its ability to identify TreMs. MRDC, on the other hand, had lower quality (median error 3.6 cm) and lower point density, making it unsuitable for TreM segmentation. Nevertheless, the study demonstrated the feasibility of augmenting the real world with virtual content at single-tree-stem level using mixed reality technology. Overall, the 3D scanning technologies presented hold great promise for recording the evolution of biodiversity at stem level.

Over the past decade, a group of lymphocyte-like cells called innate lymphoid cells (ILCs) has gained considerable attention due to their crucial role in regulating immunity and tissue homeostasis. ILCs, lacking antigen-specific receptors, are a group of functionally differentiated effector cells that act as tissue-resident sentinels against infections. Numerous studies have elucidated the characteristics of ILC subgroups, but the mechanisms controlling protective or pathological responses to pathogens still need to be better understood. This review summarizes the functions of ILCs in the immunology of infections caused by different intracellular and extracellular pathogens and discusses their possible therapeutic potential.

Using a single RGB camera to obtain accurate body dimensions, rather than measuring these manually or via more complex multicamera systems or more expensive 3D scanners, has a high application potential for the apparel industry. We present a system that estimates upper human body measurements using a hybrid set of techniques from both classic computer vision and recent machine learning. The main steps involve (1) using a camera to obtain two views (frontal and side); (2) isolating in the image pair a set of main body parts; (3) improving the image quality; (4) extracting body contours and features from the images of body parts; (5) indicating markers on these images; (6) performing a calibration step; and (7) producing refined final 3D measurements. We favour a unique geometric shape, that of an ellipse, to approximate human body main horizontal cross-sections. We focus on the more challenging parts of the body, i.e., the upper body from the head to the hips, which, we show, can be well represented by varying an ellipse’s eccentricity for each individual. Then, evaluating each fitted ellipse’s perimeter allows us to obtain better results than the current state-of-the-art methods for use in the fashion and online retail industry. In our study, we selected a set of two equations, out of many other possible choices, to best estimate upper human body section circumferences. We experimented with the system on a diverse sample of 78 female participants. The results for the upper human body measurements in comparison to the traditional manual method of tape measurements, when used as a reference, show ±1 cm average differences, which are sufficient for many applications, including online retail.

Abstract Background Schistosomiasis is a major, but generally overlooked, tropical disease carried by snails of the genus Biomphalaria, which have a large distribution in Egypt. Control of the intermediate host snail is critical in limiting schistosomiasis spread. On the topic of snails’ management, nanotechnology has gained more interest. Results Copper oxide nanoparticles, characterised by transmission electron microscopy and X-ray diffraction, showed a single crystal structure with an average crystallite size around 40 nm by X-ray diffraction and typical transmission electron microscopy (TEM) image. Also, the UV–VIS spectrophotometer displayed a sharp absorption band of CuO NPs. Molluscicidal activity of copper oxide nanoparticles against B. alexandrina snails was observed. Following exposure to CuO NPs (LC50 and LC90 was 40 and 64.3 mg/l, respectively), there was a reduction in the growth and reproductive rates of treated B. alexandrina at the sub-lethal concentrations, as well as, a drop in egg viability. Moreover, CuO NPs exhibited a toxic effect on miracidiae and cercariae of S. mansoni. Scanning electron microscopy (SEM) investigations of the head-foot and mantle of control and treated snails to the sub-lethal concentrations of CuO NPs (LC10 15.6 mg\l–LC25 27.18 mg\l) indicated morphological alterations in the ultrastructure. Conclusions CuO NPs caused a significant effect against the intermediate hosts of S. mansoni and provide a considerable scope in exploiting local indigenous resources as snail molluscicidal agents.

In the last few years, a number of low-cost 3D scanning sensors have been developed to reconstruct the real-world environment. These sensors were primarily designed for indoor use, making them highly unpredictable in terms of their performance and accuracy when used outdoors. The Azure Kinect belongs to this category of low-cost 3D scanners and has been successfully employed in outdoor applications. In addition, this sensor possesses features such as portability and live visualization during data acquisition that makes it extremely interesting in the field of forestry. In the context of forest inventory, these advantages would allow to facilitate the task of tree parameters acquisition in an efficient manner. In this paper, a protocol was established for the acquisition of 3D data in forests using the Azure Kinect. A comparison of the resulting point cloud was performed against photogrammetry. Results demonstrated that the Azure Kinect point cloud was of suitable quality for extracting tree parameters such as diameter at breast height (DBH, with a standard deviation of 2.2cm). Furthermore, the quality of the visual and geometric information of the point cloud was evaluated in terms of its feasibility to identify microhabitats. Microhabitats represent valuable information on forest biodiversity and are included in Swiss forest inventory measurements. In total, five different microhabitats were identified in the Azure Kinect Point cloud. The measurements were therefore comparable to sensors such as terrestrial laser scanning and photogrammetry. Therefore, we argue that the Azure Kinect point cloud can efficiently identify certain types of microhabitats and this study presents a first approach of its application in forest inventories.

Abstract Background Land snails, especially the chocolate banded snails, Eobania vermiculata (Muller 1774) are destructive pests of a wide range of field and vegetable crops, and biological treatment appears to be better alternative to the chemical snail control. Therefore, the goal of this work was to assess the molluscicidal activity of chitosan extracted from the crawfish Procambarus clarkii against E. vermiculata using oxidative stress, histopathological and genotoxic biomarkers. Results Exposure of snails to LC50 (222.4 mg/l) chitosan for 1, 3 and 7 days induced a significant increase in glutathione S-transferase and catalase levels then decline in reduced glutathione content after 1 and 3 days as well as a slight decrease in CAT levels, GSH content and GST of the treated snails after 7 days exposure. Histologically, the stress induced by chitosan exposure leads to deformation of cells, dilatation of the intertubular spaces, and destruction of tubules with increase in lumen size, necrosis of digestive cells with rise in vacuoles number and increase in calcium cells number. Considerably, a great damage was observed with increasing time of exposure. Furthermore, genotoxicity was assessed using RAPD-PCR technique and the results revealed that change in RAPD profiles of E. vermiculata following chitosan treatment included loss of normal DNA bands and appearance of new one compared to control snails. The genomic template stability was 63.6, 36.4 and 18.2% 1, 3 and 7 days of exposure, respectively. The apparent of new bands increased as time of exposure decreased, while GTS values decreased confirming the effect of chitosan-induced DNA damage. Conclusion Chitosan may be an ecofriendly acceptable alternative pesticide for snail control.

Abstract Background Arterial stiffness is strongly linked to the pathogenesis of heart failure and the development of acute decompensation in patients with stable chronic heart failure. This study aimed to compare arterial stiffness indices in patients with heart failure with reduced ejection fraction (HFrEF) during the acute decompensated state, and three months later after hospital discharge during the compensated state. Results One hundred patients with acute decompensated HFrEF (NYHA class III and IV) and left ventricular ejection fraction ≤ 35% were included in the study. During the initial and follow-up visits, all patients underwent full medical history taking, clinical examination, transthoracic echocardiography, and non-invasive pulse wave analysis by the Mobil-O-Graph 24-h device for measurement of arterial stiffness. The mean age was 51.6 ± 6.1 years and 80% of the participants were males. There was a significant reduction of the central arterial stiffness indices in patients with HFrEF during the compensated state compared to the decompensated state. During the decompensated state, patients presented with NYHA FC IV (n = 64) showed higher AI (24.5 ± 10.0 vs. 16.8 ± 8.6, p

Abstract Background Thelandros (Pharyngodonidae) is a gastrointestinal nematode parasite with a life cycle including lizards as main hosts. Thelandros chalcidae collected from the large intestine of the Egyptian ocellated skink, Chalcides ocellatus were described and illustrated by light and scanning electron microscopes. Seven out of fifteen (46.66%) of the examined lizards were found to be naturally infected. Also, host intestinal tissues were evaluated from hematoxylin/eosin-stained sections to describe any histopathological changes. Results Microscopic examinations revealed that the recovered pharyngodonid species characterized by mouth with triangular opening and surrounded by six simple lips, the cuticle had regular transverse annulations extending from the posterior margin of the lips to the end of the body. Male was cylindrical with distinct truncated posterior end and measured 1.59–1.86 (1.64 ± 0.10) long and 0.29–0.37 (0.32 ± 0.01) in maximum width at the level of mid-body. Female measured 1.72–2.43 (1.85 ± 0.2) long and 0.36–0.49 (0.42 ± 0.01) maximum width at the mid-body level, terminated posteriorly in a short, stout spike. Histological studies observed structural alterations represented by leukocytic infiltration, villi atrophy, and muscularis degeneration. These changes were indicative of inflammatory and degenerative reaction due to Thelandros chalcidae infection. Conclusion The present morphological study revealed that the recovered pharyngodonid species was Thelandros chalcidae causing pathological alterations in Chalcides ocellatus intestinal tissues.

Vaccination is critical to minimize serious illness and death from COVID-19. Yet uptake of COVID-19 vaccines remains highly variable, particularly among marginalized communities. This article shares lessons learned from four UNICEF interventions that supported Governments to generate acceptance and demand for COVID-19 vaccines in Zambia, Iraq, Ghana, and India. In Zambia, community rapid assessment provided invaluable real-time insights around COVID-19 vaccination and allowed the identification of population segments that share beliefs and motivations regarding COVID-19 vaccination. Findings were subsequently used to develop recommendations tailored to the different personas. In Iraq, a new outreach approach (3iS: Intensification of Integrated Immunization) utilized direct community engagement to deliver health messages and encourage service uptake, resulting in over 4.4 million doses of COVID-19 and routine immunization vaccines delivered in just 8 months. In Ghana, a human-centered design initiative was applied to co-develop community-informed strategies to improve COVID-19 vaccination rates. In India, a risk communication and community engagement initiative reached half a million people over six months, translating into a 25% increase in vaccination rates. These shared approaches can be leveraged to improve COVID-19 vaccination coverage and close gaps in routine immunization across diverse and marginalized communities.

The interrelationship among art, intelligence, and machine has important implications for the visual arts as part of a general education. Here, Frederic Fol Leymarie (FFL), a computer scientist and engineer at Goldsmiths College, and Seymour Simmons III (SS3), an artist and art educator from Winthrop University, South Carolina, discuss these issues and the value of sustained cross-disciplinary conversations in addressing challenges in the 21st century.

Despite controversy over the protective effect of the BCG (Bacille Calmette-Guérin) vaccine in preventing pulmonary tuberculosis (TB) in adults, it has been used worldwide since 1921. Although the first reports in the 1930s had noted a remarkable decrease in child mortality after BCG immunization, this could not be explained solely by a decrease in mortality from TB. These observations gave rise to the suggestion of nonspecific beneficial effects of BCG vaccination, beyond the desired protection against M. tuberculosis. The existence of an innate immunity-training mechanism based on epigenetic changes was demonstrated several years ago. The emergence of the pandemic caused by the severe acute respiratory syndrome coronavirus (SARS-CoV-2) in 2019 revived the debate about whether the BCG vaccine can affect the immune response against the virus or other unrelated pathogens. Due to the mortality of the coronavirus disease (COVID-19), it is important to verify each factor that may have a potential protective value against the severe course of COVID-19, complications, and death. This paper reviews the results of numerous retrospective studies and prospective trials which shed light on the potential of a century-old vaccine to mitigate the pandemic impact of the new virus. It should be noted, however, that although there are numerous studies intending to verify the hypothesis that the BCG vaccine may have a beneficial effect on COVID-19, there is no definitive evidence on the efficacy of the BCG vaccine against SARS-CoV-2.

Si bien la cuestión del declive en el crecimiento de población de las ciudades o Shrinking Cities, se estudia de manera más frecuente en Europa y Estados Unidos, al día de hoy en Francia presenta un interés limitado. En efecto, no existe un volumen interesante de investigación a escala nacional que haya abordado el decrecimiento de la población y sus políticas urbanas. Esto podría explicarse por la ausencia, limitado impacto o que para Francia no haya alcanzado un umbral crítico que lo convierta en una cuestión académica o política. Para validar esta hipótesis, este artículo presenta como objetivo medir el alcance del proceso de contracción de la población en las ciudades francesas. Se busca comprender si el fenómeno de Shrinking Cities, reconocido a nivel internacional, es tan marginal como sugiere el desinterés que suscita en Francia, o si su evolución actual podría considerar su existencia en el país a escala nacional.

Parapharyngodon (Oxyurida) is a lizard gastrointestinal nematode parasite with a life cycle including lizards as main hosts. However, some species are known to parasitize anurans. In the present study, P. japonicus isolated from the large intestine of the Egyptian changeable lizard, Agama mutabilis was described and illustrated. Forty five specimens of these animals were collected from south Sinai desert, Egypt during the period from May to September 2017. After necropsy, the body was opened by a longitudinal incision from vent to throat, and the gastrointestinal tract was removed. The esophagus, stomach, small and large intestines were examined separately for helminthes. The recovered nematodes were examined by light and scanning electron microscopy. Thirty six specimens (80.0 %) were found to be naturally infected. The parasite was robust with prominent cuticular transverse annulations. Mouth surrounded by three bilobed lips, each with tiny labial papillae. Three pairs of caudal papillae were observed in male worms; 1 pair precloacal, 1 pair sublateral in cloacal opening line, 1 pair in proximal region of caudal appendage on its narrowed point. The posterior extremity beard dorsally directed caudal appendages. Females were with a conical posterior end terminated at a terminal spike. Ovaries reached esophageal isthmus but not wrapped around corpus. The parasite recorded was compared morphologically and morphometrically with the most similar species, it was found that it was most similar to P. japonicus with new host and locality records.

Abstract Recent evidences suggest the involvement of DYRK1A (dual specificity tyrosine phosphorylation-regulated kinase 1 A) in Alzheimer’s disease (AD). Here we showed that DYRK1A undergoes a proteolytic processing in AD patients hippocampus without consequences on its kinase activity. Resulting truncated forms accumulate in astrocytes and exhibit increased affinity towards STAT3ɑ, a regulator of inflammatory process. These findings were confirmed in APP/PS1 mice, an amyloid model of AD, suggesting that this DYRK1A cleavage is a consequence of the amyloid pathology. We identified in vitro the Leucettine L41 as a compound able to prevent DYRK1A proteolysis in both human and mouse protein extracts. We then showed that intraperitoneal injections of L41 in aged APP/PS1 mice inhibit STAT3ɑ phosphorylation and reduce pro-inflammatory cytokines levels (IL1- β, TNF-ɑ and IL-12) associated to an increased microglial recruitment around amyloid plaques and decreased amyloid-β plaque burden. Importantly, L41 treatment improved synaptic plasticity and rescued memory functions in APP/PS1 mice. Collectively, our results suggest that DYRK1A may contribute to AD pathology through its proteolytic process, reducing its kinase specificity. Further evaluation of inhibitors of DYRK1A truncation promises a new therapeutic approach for AD.

Mosses are mainly the object of ecological and taxonomic research. This group of plants are still underestimated by scientists in other aspects of research. Recent research has shown that these plants contain remarkable and unique substances with high biological activity. Five species of mosses from a large urban ecosystem were identified for present study. In order to determine their biological potential, multifaceted studies were carried out, including: total phenolics content, antioxidant activity, antimicrobial and antifungal study, cytotoxicity evaluation, and scratch assay to assess pro-regenerative effect in the context of their possible use as the ingredients of biologically active cosmetics. Additionally, determination of individual phenolic compounds in selected extracts of the tested mosses was made. Research showed that Ceratodon purpureus and Dryptodon pulvinatus extracts had the greatest potential as antioxidants and antimicrobial activity. The cytotoxicity assessment indicated that the extracts from Dryptodon pulvinatus and Rhytidiadelphus squarossus exerted the strongest negative effect on mouse fibroblast line L929 viability at higher concentrations. While, the extract from Tortulla muralis best stimulated human foreskin fibroblast line HFF-1 proliferation and wound healing. The research on individual phenolic compounds content in the extracts tested indicated over 20 peaks on UPLC chromatograms. The conducted study has shown that mosses, especially so far unexplored species of open ecosystems, and e.g. epilytic habitats, may be a valuable source of biologically active substances and thus may constitute important medical and cosmetic possibilities.

Mycobacterium tuberculosis infections remain a global health problem in immunosuppressed patients. The effectiveness of BCG (Bacillus Calmette–Guérin), an anti-tuberculosis vaccine, is unsatisfactory. Finding a new vaccine candidate is a priority. We compared numerous immune markers in BCG-susceptible C57BL/6 and BCG-resistant C3H mice who had been injected with 0.9% NaCl (control) or with wild-type BCG or recombinant BCG secreting interleukin (IL)-18 (rBCG/IL-18) and in immunized mice who were immunocompromised with cyclophosphamide (CTX). The inoculation of rBCG/IL-18 in immunocompetent mice increased the percentage of bone marrow myeloblasts and promyelocytes, which were further elevated in the rBCG/IL-18/CTX-treated mice: C57BL/6 mice—3.0% and 11.4% (control) vs. 18.6% and 42.4%, respectively; C3H mice—1.1% and 7.7% (control) vs. 18.4% and 44.9%, respectively, p < 0.05. The bone marrow cells showed an increased mean fluorescence index (MFI) in the CD34 adhesion molecules: C57BL/6 mice—4.0 × 103 (control) vs. 6.2 × 103; C3H mice—4.0 × 103 (control) vs. 8.0 × 103, p < 0.05. Even in the CTX-treated mice, the rBCG/IL-18 mobilized macrophages for phagocytosis, C57BL/6 mice—4% (control) vs. 8%; C3H mice—2% (control) vs. 6%, and in immunocompetent mice, C57BL/6 induced the spleen homing of effector memory CD4+ and CD8+ T cells (TEM), 15% (control) vs. 28% and 8% (control) vs. 22%, respectively, p < 0.05. In conclusion, rBCG/IL-18 effectively induced selected immune determinants that were maintained even in immunocompromised mice.

Cytokine receptors are critical regulators of the antimycobacterial immune response, playing a key role in initiating and coordinating the recruitment and activation of immune cells during infection. They recognize and bind specific cytokines and are involved in inducing intracellular signal transduction pathways that regulate a diverse range of biological functions, including proliferation, differentiation, metabolism and cell growth. Due to mutations in cytokine receptor genes, defective signaling may contribute to increased susceptibility to mycobacteria, allowing the pathogens to avoid killing and immune surveillance. This paper provides an overview of cytokine receptors important for the innate and adaptive immune responses against mycobacteria and discusses the implications of receptor gene defects for the course of mycobacterial infection.

The angular distribution of the dimuon system of the decays.

This paper presents the network bending framework, a new approach for manipulating and interacting with deep generative models. We present a comprehensive set of deterministic transformations that can be inserted as distinct layers into the computational graph of a trained generative neural network and applied during inference. In addition, we present a novel algorithm for analysing the deep generative model and clustering features based on their spatial activation maps. This allows features to be grouped together based on spatial similarity in an unsupervised fashion. This results in the meaningful manipulation of sets of features that correspond to the generation of a broad array of semantically significant features of the generated results. We outline this framework, demonstrating our results on deep generative models for both image and audio domains. We show how it allows for the direct manipulation of semantically meaningful aspects of the generative process as well as allowing for a broad range of expressive outcomes.

Although the therapeutic effect of mycobacteria as antitumor agents has been known for decades, recent epidemiological and experimental studies have revealed that mycobacterium-related chronic inflammation may be a possible mechanism of cancer pathogenesis. Mycobacterium tuberculosis and non-tuberculous Mycobacterium avium complex infections have been implicated as potentially contributing to the etiology of lung cancer, whereas Mycobacterium ulcerans has been correlated with skin carcinogenesis. The risk of tumor development with chronic mycobacterial infections is thought to be a result of many host effector mechanisms acting at different stages of oncogenesis. In this paper, we focus on the nature of the relationship between mycobacteria and cancer, describing the clinical significance of mycobacteria-based cancer therapy as well as epidemiological evidence on the contribution of chronic mycobacterial infections to the increased lung cancer risk.