1. Direct interaction between metastasis-associated protein 1 and endophilin 3
- Author
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Aramaki, Yuko, Ogawa, Katsuhiro, Toh, Yasushi, Ito, Takahiro, Akimitsu, Nobuyoshi, Hamamoto, Hiroshi, Sekimizu, Kazuhisa, Matsusue, Kimihiko, Kono, Akira, Iguchi, Haruo, and Takiguchi, Soichi
- Subjects
METASTASIS ,CANCER invasiveness ,CYTOPLASM ,STEROID hormones - Abstract
Abstract: The yeast two-hybrid system was used to search for partners of mouse metastasis-associated protein 1 (Mta1). Screening of a cDNA library prepared from mouse embryo yielded positive clones coding for endophilin 3. The site of interaction was suggested to be the SH-3-binding domain of Mta1 and SH-3 domain of endophilin 3. This interaction was confirmed by GST pull-down assay in vitro and immunoprecipitation in vivo. The Mta1 and endophilin 3 transcripts were highly expressed in testis and brain. But, Mta1 localized mainly in nucleus and to a lesser extent in cytoplasm while endophilin 3 localized mainly in cytoplasm. If Mta1 functions in cytoplasm, it might be involved in the regulation of endocytosis mediated by endophilin 3. [Copyright &y& Elsevier]
- Published
- 2005
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