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Direct interaction between metastasis-associated protein 1 and endophilin 3
- Source :
- FEBS Letters; Jul2005, Vol. 579 Issue 17, p3731-3736, 6p
- Publication Year :
- 2005
-
Abstract
- Abstract: The yeast two-hybrid system was used to search for partners of mouse metastasis-associated protein 1 (Mta1). Screening of a cDNA library prepared from mouse embryo yielded positive clones coding for endophilin 3. The site of interaction was suggested to be the SH-3-binding domain of Mta1 and SH-3 domain of endophilin 3. This interaction was confirmed by GST pull-down assay in vitro and immunoprecipitation in vivo. The Mta1 and endophilin 3 transcripts were highly expressed in testis and brain. But, Mta1 localized mainly in nucleus and to a lesser extent in cytoplasm while endophilin 3 localized mainly in cytoplasm. If Mta1 functions in cytoplasm, it might be involved in the regulation of endocytosis mediated by endophilin 3. [Copyright &y& Elsevier]
- Subjects :
- METASTASIS
CANCER invasiveness
CYTOPLASM
STEROID hormones
Subjects
Details
- Language :
- English
- ISSN :
- 00145793
- Volume :
- 579
- Issue :
- 17
- Database :
- Complementary Index
- Journal :
- FEBS Letters
- Publication Type :
- Academic Journal
- Accession number :
- 18094628
- Full Text :
- https://doi.org/10.1016/j.febslet.2005.05.069