1. Oleiferoside W from the roots of Camellia oleifera C. Abel, inducing cell cycle arrest and apoptosis in A549 cells.
- Author
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Wu, Jiang-Ping, Kang, Nai-Xin, Zhang, Mi-Ya, Gao, Hong-Wei, Li, Xiao-Ran, Liu, Yan-Li, Xu, Qiong-Ming, and Yang, Shi-Lin
- Subjects
ANIMAL experimentation ,ANTINEOPLASTIC agents ,APOPTOSIS ,CELL culture ,CELL cycle ,CELL lines ,GLYCOSIDES ,IMMUNOBLOTTING ,LUNG cancer ,RESEARCH funding ,PLANT roots ,PLANT extracts ,CELL survival ,ONE-way analysis of variance ,PHARMACODYNAMICS - Abstract
Camellia oleifera C. Abel has been widely cultivated in China, and a group of bioactive constituents such as triterpeniod saponin have been isolated from C. oleifera C. Abel. In the current study, a new triterpeniod saponin was isolated from the EtOH extract of the roots of C. oleifera C. Abel, named as oleiferoside W, and the cytotoxic properties of oleiferoside W were evaluated in non-small cell lung cancer A549 cells. At the same time the inducing apoptosis, the depolarization of mitochondrial membrane potential (Δψ), the up-regulation of related pro-apoptotic proteins, such as cleaved-PARP, cleaved-caspase-3, and the down-regulation of anti-apoptotic marker Bcl-2/Bax were measured on oleiferoside W. Furthermore, the function, inducing the generation of reactive oxygen species (ROS) and apoptosis, of oleiferoside W could be reversed by N-acetylcysteine (NAC). In conclusion, our findings showed that oleiferoside W induced apoptosis involving mitochondrial pathway and increasing intracellular ROS production in the A549 cells, suggesting that oleiferoside W may have the possibility to be a useful anticancer agent for therapy in lung cancer. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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