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Avian eggs develop outside of the female body, and therefore embryonic development is subject to multiple internal (physiological) and external (ecological) factors. Embryonic developmental rate has important consequences for survival. Within species, embryos that develop too quickly often experience deformities, disorders, or mortality, while embryos that develop slowly risk inviability and increase the time they are exposed to various sources of mortality in the nest. These contrasting forces may lead to interspecific variation in developmental rates. We investigated potential factors affecting embryonic heart rate (EHR), a proxy of development, across 14 passerine species in the field. More specifically, we investigated if nest predation risk, clutch size, seasonality, and egg volume influenced EHR. From previous research, we expected, and found, that EHR was positively associated with embryonic age and egg temperature. Species with greater nest predation risk had higher EHR, shorter incubation periods, and lower nest temperature variance. EHR increased as the season progressed and with egg volume, while EHR declined with clutch size. Bird species exhibit varying strategies to increase nestling and fledgling survival in response to predation risk, and these results suggest that variation in embryonic development may be related to species‐specific differences in nest predation risk. [ABSTRACT FROM AUTHOR]

Copyright of Avian Conservation & Ecology is the property of Resilience Alliance and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Abstract Avian eggs develop outside of the female body, and therefore embryonic development is subject to multiple internal (physiological) and external (ecological) factors. Embryonic developmental rate has important consequences for survival. Within species, embryos that develop too quickly often experience deformities, disorders, or mortality, while embryos that develop slowly risk inviability and increase the time they are exposed to various sources of mortality in the nest. These contrasting forces may lead to interspecific variation in developmental rates. We investigated potential factors affecting embryonic heart rate (EHR), a proxy of development, across 14 passerine species in the field. More specifically, we investigated if nest predation risk, clutch size, seasonality, and egg volume influenced EHR. From previous research, we expected, and found, that EHR was positively associated with embryonic age and egg temperature. Species with greater nest predation risk had higher EHR, shorter incubation periods, and lower nest temperature variance. EHR increased as the season progressed and with egg volume, while EHR declined with clutch size. Bird species exhibit varying strategies to increase nestling and fledgling survival in response to predation risk, and these results suggest that variation in embryonic development may be related to species‐specific differences in nest predation risk.

Noise pollution can degrade the behavioral, physiological, and psychological health of humans and other creatures. We used breeding pairs of Eastern Bluebirds ( Sialia sialis ) to assess behavioral and reproductive responses to both chronic roadway noise and experimental intermittent playbacks of construction noise. Active nests in boxes placed near and far from large roads were randomly assigned as treatments or controls for experimental playbacks during incubation. Using temperature signatures from iButtons placed within nest cups we quantified certain female incubation behaviors (# and length of bouts, # of small temperature fluctuations, and total warming minutes per day) and hatching success was recorded for 40 nests in spring of 2019. Nests in quiet areas that received no additional playback treatments of construction noise had markedly higher nest success than any exposed to noise. Nests exposed to chronic traffic noise only, and quiet nests that received 3–4 days of construction noise had the lowest hatching success. Females in traffic-quiet nests increased restlessness (small temperature fluctuations) and experienced decreasing hatching success as the number of days of construction noise playback increased. Thus, birds choosing either quiet or noisy boxes had contrasting responses to bouts of construction noise. Other female incubation behaviors we could detect were unaffected by noise but changed in expected ways with seasonal progression. In sum, both types of noise can decrease hatch rate, but with intermittent noise this is likely due to female restlessness, or too many small drops in temperature to maintain optimal embryo development.

Weather conditions play a pivotal role in embryo development and parental incubation costs, potentially impacting the clutch size and incubation behavior of birds. Understanding these effects is crucial for bird conservation. Reeves's Pheasant (Syrmaticus reevesii) is a threatened species endemic to China, which is characterized by female-only incubation. However, there is a lack of information regarding the impact of weather conditions on clutch size and incubation behavior in this species. Using satellite tracking, we tracked 27 wild female Reeves's Pheasants from 2020 to 2023 in Hubei Province, China. We explored their clutch size and incubation behavior, as well as their responses to ambient temperature and precipitation. Clutch size averaged 7.75 ± 1.36, had an association with average ambient temperature and average daily precipitation during the egg-laying period, and was potentially linked to female breeding attempts. Throughout the incubation period, females took an average of 0.73 ± 0.46 recesses every 24 h, with an average recess duration of 100.80 ± 73.37 min and an average nest attendance of 92.98 ± 5.27%. They showed a unimodal recess pattern in which nest departures peaked primarily between 13:00 and 16:00. Furthermore, females rarely left nests when daily precipitation was high. Recess duration and nest attendance were influenced by the interaction between daily mean ambient temperature and daily precipitation, as well as day of incubation. Additionally, there was a positive correlation between clutch size and recess duration. These results contribute valuable insights into the life-history features of this endangered species.

The incubation behavior of the Japanese Nagoya chicken breed is a commercial issue because it often causes a sudden and sharp drop in egg production. In this study, whether the incidence of incubation behavior in Nagoya laying hens was associated with calls and the presence of roosters in the same laying house was investigated. Four experiments were conducted using commercial layer-type Nagoya hens where the hatching time of the experimental birds and the treatment order in the presence of males were changed . In Experiment 1, the proportion of incubation behavior in the presence of roosters kept in another pen located between pen-rearing hens (51.3%) was higher than that in their absence (15.9%) or with only rooster calls (23.8%). In Experiments 2, 3, and 4, the proportion of incubation behavior in the presence of roosters (47.3%, 33.3%, and 37.9%, respectively) was higher than that in their absence (33.3%, 17.4%, and 25.6%, respectively). In all experiments, approximately 70% of the incubating hens observed in the absence of roosters exhibited incubation behavior, even in the presence of roosters. Therefore, the presence of roosters may enhance egg incubation behavior in Nagoya laying hens.

The incubation behavior of geese seriously affects their egg production performance. Studies on incubation behavior have identified functional genes, but the regulatory architecture relationship between functional genes and chromatin accessibility remains poorly understood. Here, we present an integrated analysis of open chromatin profiles and transcriptome to identify the cis-regulatory element and their potential transcription factors involved in regulating incubation behavior in goose pituitary. Assay for transposase-accessible chromatin sequencing (ATAC-seq) revealed that open chromatin regions increased in the pituitary during the transition from incubation behavior to laying. We identified 920 significant differential accessible regions (DARs) in the pituitary. Compared to the laying stage, most DARs had higher chromatin accessibility in the brooding stage. Motif analysis of open DARs showed that the most significant transcription factor (TF) occupied sites predominantly enriched in motifs binding to the RFX family (RFX5, RFX2, and RFX1). While the majority of TF motifs enriched under sites of the nuclear receptor (NR) family (ARE, GRE, and PGR) in closed DARs at the incubation behavior stage. Footprint analysis indicated that the transcription factor RFX family exhibited higher binding on chromatin at the brooding stage. To further elucidate the effect of changes in chromatin accessibility on gene expression levels, a comparison of the transcriptome revealed 279 differentially expressed genes (DEGs). The transcriptome changes were associated with processes of steroid biosynthesis. By integrating ATAC-seq and RNA-seq, few DARs directly affect incubation behavior by regulating the transcription levels of genes. Five DAR-related DEGs were found to be closely related to maintaining the incubation behavior in geese. Footprinting analysis revealed a set of transcription factors (RFX1, RFX2, RFX3, RFX5, BHLHA15, SIX1, and DUX) which displayed the highest activity at the brooding stage. SREBF2 was predicted to be the unique differentially expressed transcription factor whose mRNA level was down-regulated and enriched in hyper-accessible regions of PRL in the broody stage. In the present study, we comprehensively profiled the transcriptome and chromatin accessibility in the pituitary related to incubation behavior. Our findings provided insight into the identification and analysis of regulatory elements in goose incubation behavior. The epigenetic alterations profiled here can help decipher the epigenetic mechanisms that contribute to the regulation of incubation behavior in birds. [ABSTRACT FROM AUTHOR]

Urbanization is currently considered one of the most rapid types of global environmental change. Urban habitats are biotically and abiotically different from their rural areas, i.e., the ambient temperature, predator, and food availability. These novel challenges create new selection pressures, which allow one to investigate eco-evolutionary responses to contemporary environmental change. A total of 118 breeding nests were monitored for nest predation in both urban and rural areas from 2018 to 2020. We used environmental factors from urban and rural areas and behavioral data from 439 Chinese Blackbird (Turdus mandarinus) valid incubation days to understand the impact of urbanization on the incubation behavior of blackbirds and its adaptation mechanism to the urban environment. Cities have warmer ambient temperatures and lower predation pressures than rural areas. Urban blackbirds chose the incubation strategy with shorter and more bouts, while rural blackbirds selected the incubation strategy with longer and fewer bouts. The plasticity of incubation behavior of urban blackbirds was higher than that of rural areas, and the range of egg temperature was also higher than that of rural areas. In addition, incubation temperature and the number of bouts per day were the key factors affecting the day survival rate of blackbirds, and the hatching rate of urban blackbirds was higher than that of rural blackbirds. Our results provide evidence for behavioral shifts in blackbirds during adaptation to urbanization and support the central role of behavioral adaptation in the successful colonization of new environments by wildlife. These help us understand the behavioral characteristics required for wildlife to live in cities and the urban adaptors faced environmental pressures.

Avian reproductive behavior is regulated through the neuroendocrine system. The transition from laying to brooding is strictly controlled by the hypothalamus-pituitary-gonadal (HPG) axis. Cross talk on the HPG axis relies on the circulatory system, where the dynamics of serum proteins can be observed during different reproductive phases. Some canonical hormones, such as prolactin and luteinizing hormone, play important roles in the transition through reproductive phases. However, little is known at the whole-proteome level. To discover novel serum proteins, we employed isobaric tags for relative and absolute quantification to assay the serum proteome during different reproductive phases in chicken. We identified a total of 1,235 proteins from chicken serum; 239 of these proteins showed differential expression between the laying and brooding stages, including a low concentration of steroid metabolism-related proteins and a high concentration of calcium signaling-related proteins (fold change ≥1.5 or ≤0.66; P

Abstract In ground nesting upland birds, reproductive activities contribute to elevated predation risk, so females presumably use multiple strategies to ensure nest success. Identification of drivers reducing predation risk has primarily focused on evaluating vegetative conditions at nest sites, but behavioral decisions manifested through movements during incubation may be additional drivers of nest survival. However, our understanding of how movements during incubation impact nest survival is limited for most ground nesting birds. Using GPS data collected from female Eastern Wild Turkeys (n = 206), we evaluated nest survival as it relates to movement behaviors during incubation, including recess frequency, distance traveled during recesses, and habitat selection during recess movements. We identified 9,361 movements off nests and 6,529 recess events based on approximately 62,065 hr of incubation data, and estimated mean nest attentiveness of 84.0%. The numbers of recesses taken daily were variable across females (range: 1‒7). Nest survival modeling indicated that increased cumulative distance moved during recesses each day was the primary driver of positive daily nest survival. Our results suggest behavioral decisions are influencing trade‐offs between nest survival and adult female survival during incubation to reduce predation risk, specifically through adjustments to distances traveled during recesses.

Natural gas compressor stations emit loud, low-frequency noise that travels hundreds of meters into undisturbed habitat. We used experimental playback of natural gas compressor noise to determine whether and how noise influenced settlement decisions and reproductive output as well as when in the nesting cycle birds were most affected by compressor noise. We established 80 nest boxes to attract Eastern Bluebirds (Sialia sialis) and Tree Swallows (Tachycineta bicolor) to locations where they had not previously nested and experimentally introduced shale gas compressor noise to half the boxes while the other 40 boxes served as controls. Our experimental design allowed us to control for the confounding effects of both physical changes to the environment associated with compressor stations as well as site tenacity or the tendency for birds to return to the specific locations where they had previously bred. We incorporated behavioral observations with video cameras placed within boxes to determine how changes in behavior might lead to any noted changes in fitness. Neither species demonstrated a preference for box type (quiet or noisy), and there was no difference in clutch size between box types. In both species, we observed a reduction in incubation time, hatching success, and fledging success (proportion of all eggs that fledged) between quiet and noisy boxes but no difference in provisioning rates. Nest success (probability of fledging at least one young; calculated from all nests that were initiated) was not affected by noise in either species suggesting that noise did not increase rates of either depredation or abandonment but instead negatively impacted fitness through reduced hatching and fledging success. Compressor noise caused behavioral changes that led to reduced reproductive success; for Eastern Bluebirds and Tree Swallows, gas infrastructure can create an equal-preference ecological trap where birds do not distinguish between lower and higher quality territories even when they incur fitness costs. [ABSTRACT FROM AUTHOR]

In ground nesting upland birds, reproductive activities contribute to elevated predation risk, so females presumably use multiple strategies to ensure nest success. Identification of drivers reducing predation risk has primarily focused on evaluating vegetative conditions at nest sites, but behavioral decisions manifested through movements during incubation may be additional drivers of nest survival. However, our understanding of how movements during incubation impact nest survival is limited for most ground nesting birds. Using GPS data collected from female Eastern Wild Turkeys (n = 206), we evaluated nest survival as it relates to movement behaviors during incubation, including recess frequency, distance traveled during recesses, and habitat selection during recess movements. We identified 9,361 movements off nests and 6,529 recess events based on approximately 62,065 hr of incubation data, and estimated mean nest attentiveness of 84.0%. The numbers of recesses taken daily were variable across females (range: 1‒7). Nest survival modeling indicated that increased cumulative distance moved during recesses each day was the primary driver of positive daily nest survival. Our results suggest behavioral decisions are influencing trade‐offs between nest survival and adult female survival during incubation to reduce predation risk, specifically through adjustments to distances traveled during recesses. [ABSTRACT FROM AUTHOR]

The incubation behavior of the Japanese Nagoya chicken breed is a commercial issue because it often causes a sudden and sharp drop in egg production. In this study, whether the incidence of incubation behavior in Nagoya laying hens was associated with calls and the presence of roosters in the same laying house was investigated. Four experiments were conducted using commercial layer-type Nagoya hens where the hatching time of the experimental birds and the treatment order in the presence of males were changed . In Experiment 1, the proportion of incubation behavior in the presence of roosters kept in another pen located between pen-rearing hens (51.3%) was higher than that in their absence (15.9%) or with only rooster calls (23.8%). In Experiments 2, 3, and 4, the proportion of incubation behavior in the presence of roosters (47.3%, 33.3%, and 37.9%, respectively) was higher than that in their absence (33.3%, 17.4%, and 25.6%, respectively). In all experiments, approximately 70% of the incubating hens observed in the absence of roosters exhibited incubation behavior, even in the presence of roosters. Therefore, the presence of roosters may enhance egg incubation behavior in Nagoya laying hens., Competing Interests: Conflicts of Interest: The authors declare no conflict of interest., (2023 Japan Poultry Science Association.)

The molecular mechanism underlying in the onset and maintenance of incubation behavior are not fully understood, and it is still unknown the reason why White Leghorn, a layer strain, hens never display incubation behavior. Therefore, to explore specific hypothalamic genes regulating incubation behavior, cap analysis of gene expression (CAGE) were applied to comparison between incubating Silkie and laying White Leghorn hens. In addition, mRNA expression of some differentially expressed genes (DEGs) and melanocortinergic appetite genes including agouti‐related peptide (AgRP) and pro‐opiomelanocortin (POMC) was also analyzed on Silkie hens under natural anorexia and starvation. The CAGE identified 217 hypothalamic DEGs in incubating Silkie hens, and that of two, transthyretin (TTR) and prolactin‐releasing peptide (PrRP), suggested as appetite gene, were markedly up– and down‐regulated in incubating hens, respectively. In addition, AgRP and POMC expression also increased in incubating bird. mRNA expression of TTR, PrRP, and appetite genes were not differed significantly by starvation, although TTR mRNA expression was relatively high in fasting hens. Consequently, transcriptome by CAGE identified a number of hypothalamic genes differentially expressed by incubation behavior in Silkie hens. Of these, it is suggested that TTR and PrRP may, at least in part, be related to adaptation to natural anorexia in incubating Silkie chickens. [ABSTRACT FROM AUTHOR]

For organisms living in seasonal environments, synchronizing the peak energetic demands of reproduction with peak food availability is a key challenge. Understanding the extent to which animals can adjust behavior to optimize reproductive timing, and the cues they use to do this, is essential for predicting how they will respond to future climate change. In birds, the timing of peak energetic demand is largely determined by the timing of clutch initiation; however, considerable alterations can still occur once egg laying has begun. Here, we use a wild population of great tits ( Parus major) to quantify individual variation in different aspects of incubation behavior (onset, duration, and daily intensity) and conduct a comprehensive assessment of the causes and consequences of this variation. Using a 54-year dataset, we demonstrate that timing of hatching relative to peak prey abundance (synchrony) is a better predictor of reproductive success than clutch initiation or clutch completion timing, suggesting adjustments to reproductive timing via incubation are adaptive in this species. Using detailed in-nest temperature recordings, we found that postlaying, birds improved their synchrony with the food peak primarily by varying the onset of incubation, with duration changes playing a lesser role. We then used a sliding time window approach to explore which spring temperature cues best predict variance in each aspect of incubation behavior. Variation in the onset of incubation correlated with mean temperatures just prior to laying; however, incubation duration could not be explained by any of our temperature variables. Daily incubation intensity varied in response to daily maximum temperatures throughout incubation, suggesting female great tits respond to temperature cues even in late stages of incubation. Our results suggest that multiple aspects of the breeding cycle influence the final timing of peak energetic demand. Such adjustments could compensate, in part, for poor initial timing, which has significant fitness impacts. [ABSTRACT FROM AUTHOR]

Urbanization is currently considered one of the most rapid types of global environmental change. Urban habitats are biotically and abiotically different from their rural areas, i.e., the ambient temperature, predator, and food availability. These novel challenges create new selection pressures, which allow one to investigate eco-evolutionary responses to contemporary environmental change. A total of 118 breeding nests were monitored for nest predation in both urban and rural areas from 2018 to 2020. We used environmental factors from urban and rural areas and behavioral data from 439 Chinese Blackbird (Turdus mandarinus) valid incubation days to understand the impact of urbanization on the incubation behavior of blackbirds and its adaptation mechanism to the urban environment. Cities have warmer ambient temperatures and lower predation pressures than rural areas. Urban blackbirds chose the incubation strategy with shorter and more bouts, while rural blackbirds selected the incubation strategy with longer and fewer bouts. The plasticity of incubation behavior of urban blackbirds was higher than that of rural areas, and the range of egg temperature was also higher than that of rural areas. In addition, incubation temperature and the number of bouts per day were the key factors affecting the day survival rate of blackbirds, and the hatching rate of urban blackbirds was higher than that of rural blackbirds. Our results provide evidence for behavioral shifts in blackbirds during adaptation to urbanization and support the central role of behavioral adaptation in the successful colonization of new environments by wildlife. These help us understand the behavioral characteristics required for wildlife to live in cities and the urban adaptors faced environmental pressures. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Field Ornithology is the property of Resilience Alliance and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Field Ornithology is the property of Resilience Alliance and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

We provide the first detailed nesting biology information for the Yellow-breasted Warbling-Antbird (Hypocnemis subflava), a member of the recently split Warbling Antbird complex. We found seven pouch-shaped nests with two eggs or nestlings within Manu National Park, SE Peru. Long incubation sessions (93.7 ± 7.78 minutes, range = 4-480 minutes, n = 18 days) were untaken by both parents and high diurnal nest attentiveness (% of time on the nest) averaged 85.8 ± 1.4% (n = 16) as a result of taking fewer short foraging trips (6.3 ± 0.6 trips/day, range = 3-11; n = 14) that lasted 16.19 ± 1.78 min (range = 1-91; n = 18). Incubation behavior produced an average inner nest temperature of 32.3 ± 0.24 °C (n = 15 days) and average egg temperature of 36.4 ± 0.09 °C (n = 3). Individual nests at different elevations exhibit differences in incubation behavior. The nestling period was 11 days (n = 1) and both parents brooded and fed the nestlings. Nest shape, location, and composition were similar to other species in the complex, but egg coloration was variable among species. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Field Ornithology is the property of Resilience Alliance and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The article discusses a 2010 study to determine what factors during incubation have an effect on Wood Ducks, Aix sponsa in Latin. The researchers manipulated the incubation of females to find out how nest temperature, female body mass and incubation behavior affected their reproduction. They found no relationship between how long females incubated and how much body mass they lost after, but that the earlier they incubated, the more weight they lost, while the later they incubated, the more mass they maintained after.

This article discusses a study which examined the incubation behavior of the Blood Pheasant (Ithaginis cruentus), a uniparental bird of high-elevation forests at Lianhaushan Nature Reserve in southern Gansu Province in China. Using temperature loggers to study the behavior, researchers learned that egg predation was not associated with movements of females to and from nests. They also discovered that egg temperatures dropped rapidly once females left the nest.

Copyright of Journal of Field Ornithology is the property of Resilience Alliance and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Many species of birds start incubation before the last egg of a clutch is laid, which often results in asynchronous hatching and fledging. The incubation pattern favored by natural selection should lead to greater reproductive success than alternative patterns. Equations for calculating the reproductive success of a clutch as a function of the start of incubation have been proposed by Clark and Wilson (1981) and Hussell (1985). Both equations predicted a greater frequency of an earlier start of incubation than occurs in nature. Here, I present a new equation in which the relevant parameters determining reproductive success as a function of the start of incubation are (1) the probability of any young leaving a nest and (2) the post-first-fledging daily survival rates of the remaining nestlings. This equation is universally applicable and accounts for the greater frequency of incubation starting later, rather than earlier, in the laying sequence of most birds. I use the equation to evaluate various hypotheses regarding the evolution of incubation patterns. [ABSTRACT FROM AUTHOR]

Abstract: We investigated whether polychlorinated biphenyl (PCB) exposure in American kestrels (Falco sparverius) influenced incubation behavior and whether altered behavior could lead to poor reproductive success. Captive kestrels were fed a mixture of PCBs (Aroclors 1248:1254:1260) at an approximate daily dose of 7mg/kg body weight, 1 month prior to pairing and throughout incubation. Behaviors of 23 control and 23 PCB-exposed pairs were monitored throughout incubation using an electronic balance in the nest box. PCB exposure resulted in longer incubation periods and in altered incubation behaviors. Seven of 14 behavioral variables showed some association with treatment, with sex-specific effects largely biased toward disrupted male behavior. For most behaviors, the treatment effect was explained by the delayed clutch initiation induced by PCBs rather than by a direct physiological impact of the contaminants. PCB-exposed pairs with greater attendance to their eggs and better coordination of incubation duties had improved hatching success. [Copyright &y& Elsevier]

Reproduction in birds requires the input of time and energy during discrete breeding phases leading to investment trade-offs between laying date, clutch size, body mass, and incubation constancy. We investigated costs during incubation by experimentally enlarging 25 clutches of white-tailed ptarmigan Lagopus leucurus. The experiment was conducted in 2 years, one with harsh weather that forced a natural delay in reproduction. When forced to delay egg-laying, females began incubation with poorer body condition and foraged more during incubation. Rates of mass loss during incubation were not affected by clutch enlargement, and did not differ between harsh and benign years; however, females that were heavier at the start of incubation lost more mass than lighter females. Clutch-enlarged females had reduced nest attendance compared to control birds in both years and incubation periods increased by up to 2 days relative to controls. In the harsh year, there was a trend for clutch-enlarged females to have lower nest success, but there was no effect on overwinter survival. Different behavioral responses by females in the 2 years showed that incubation costs may depend on other factors such as female quality, food supply, or weather conditions. Incubation is a dynamic period during which birds may adjust energy balances by varying body condition and food intake. [ABSTRACT FROM AUTHOR]

Avian reproductive behavior is regulated through the neuroendocrine system. The transition from laying to brooding is strictly controlled by the hypothalamus-pituitary-gonadal (HPG) axis. Cross talk on the HPG axis relies on the circulatory system, where the dynamics of serum proteins can be observed during different reproductive phases. Some canonical hormones, such as prolactin and luteinizing hormone, play important roles in the transition through reproductive phases. However, little is known at the whole-proteome level. To discover novel serum proteins, we employed isobaric tags for relative and absolute quantification to assay the serum proteome during different reproductive phases in chicken. We identified a total of 1,235 proteins from chicken serum; 239 of these proteins showed differential expression between the laying and brooding stages, including a low concentration of steroid metabolism-related proteins and a high concentration of calcium signaling-related proteins (fold change ≥1.5 or ≤0.66; P < 0.05). Pathway analysis and protein-protein interaction networks predicated the difference in follicle development between the brooding stage and laying stages and were related to the 14-3-3 protein family, which is associated with oocyte meiosis and maturation. Together, these results provided a proteomics foundation for investigating the dynamic changes taking place in the circulatory system during reproductive phase transition, and also uncovered new insights regarding follicle development that underlie the avian reproductive cycle., (Copyright © 2020. Published by Elsevier Inc.)

The use of feathers as nest material has been proposed as a kind of self-medication strategy because antimicrobial-producing microorganisms living on feathers may defend offspring against pathogenic infections. In this case, it is expected that density of antimicrobial-producing bacteria, and their antimicrobial effects, are higher in feathers that line the nests than in eggshells. Moreover, we know that feather pigmentation and breeding activity may influence density and antimicrobial production of bacteria. To test these predictions, we analyzed bacterial densities and antimicrobial activity of bacterial colonies isolated from bird eggshells and nest-lining feathers against bacterial strains comprising potential pathogens. Samples were collected from spotless starling (Sturnus unicolor) nests, and from artificial nests to isolate the effects of breeding activity on bacterial communities. The composition of feathers lining the nests was experimentally manipulated to create groups of nests with pigmented feathers, with unpigmented feathers, with both types of feathers or without feathers. Although we did not detect an effect of experimental feather treatments, we found that bacterial colonies isolated from feathers were more active against the tested bacterial strains than those isolated from eggshells. Moreover, bacterial density on feathers, keratinolytic bacteria on eggshells and antimicrobial activity of colonies isolated were higher in starling nests than in artificial nests. These results suggest that antimicrobial activity of bacteria growing on nest-lining feathers would be one of the mechanisms explaining the previously detected antimicrobial effects of this material in avian nests, and that breeding activity results in nest bacterial communities with higher antimicrobial activity. [ABSTRACT FROM AUTHOR]

Nest attentiveness (percentage of time spent on the nest) during incubation represents a parent-offspring conflict; incubating birds must balance a trade-off between caring for embryos by staying on the nest versus caring for themselves by getting off the nest to forage. For species in which females are the sole incubator, males can potentially affect this trade-off and increase nest attentiveness by feeding incubating females on the nest (incubation feeding). Increased nest attentiveness may be required when local microclimate conditions are harsh and thereby require greater incubation feeding (microclimate hypothesis). Alternatively, incubation feeding may be constrained by risk of attracting nest predators (nest predation hypothesis), which in turn may constrain female nest attentiveness because of energy limitation. We show that incubation feeding rates are much greater among cavity-nesting than among coexisting open-nesting birds. Under the microclimate hypothesis, the greater incubation feeding rates of cavity-nesting birds generate the prediction that microclimate should be harsher than for open-nesting birds. Our results reject this hypothesis because we found the opposite pattern; cavity-nesting birds experienced more moderate (less variable) microclimates that were less often below temperatures (i.e., 16°C) that can negatively impact eggs compared with open-nesting species. In contrast, incubation feeding rates were highly negatively correlated with nest predation both within and between the two nest types, supporting the nest predation hypothesis. Incubation feeding in turn was positively correlated with nest attentiveness. Thus, nest predation may indirectly affect female incubation behavior by directly affecting incubation feeding by the male.