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5 results on '"Xiang-Yue Zeng"'

1. Intranasal delivery of replicating mRNA encoding neutralizing antibody against SARS-CoV-2 infection in mice

Author

Jia-Qi Li, Zhe-Rui Zhang, Hong-Qing Zhang, Ya-Nan Zhang, Xiang-Yue Zeng, Qiu-Yan Zhang, Cheng-Lin Deng, Xiao-Dan Li, Bo Zhang, and Han-Qing Ye

Subjects
Medicine, Biology (General), QH301-705.5
Abstract

Abstract The lung is the prophylaxis target against SARS-CoV-2 infection, and neutralizing antibodies are a leading class of biological products against various infectious viral pathogen. In this study, we develop a safe and cost-effective platform to express neutralizing antibody in the lung with replicating mRNA basing on alphavirus replicon particle (VRP) delivery system, to prevent SARS-CoV-2 infections. First, a modified VEEV replicon with two subgenomic (sg) promoters was engineered to translate the light and heavy chains of antibody simultaneously, for expression and assembly of neutralizing anti-SARS-CoV-2 antibody CB6. Second, the feasibility and protective efficacy of replicating mRNA against SARS-CoV-2 infection were demonstrated through both in vitro and in vivo assays. The lung target delivery with the help of VRP system resulted in efficiently block SARS-CoV-2 infection with reducing viral titer and less tissue damage in the lung of mice. Overall, our data suggests that expressing neutralizing antibodies in the lungs with the help of self-replicating mRNA could potentially be a promising prophylaxis approach against SARS-CoV-2 infection.

Published
2021
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2. Rational design of West Nile virus vaccine through large replacement of 3′ UTR with internal poly(A)

Author

Ya‐Nan Zhang, Na Li, Qiu‐Yan Zhang, Jing Liu, Shun‐Li Zhan, Lei Gao, Xiang‐Yue Zeng, Fang Yu, Hong‐Qing Zhang, Xiao‐Dan Li, Cheng‐Lin Deng, Pei‐Yong Shi, Zhi‐Ming Yuan, Shao‐Peng Yuan, Han‐Qing Ye, and Bo Zhang

Subjects
flavivirus, internal poly(A), live‐attenuated vaccine, UTR, West Nile Virus, Medicine (General), R5-920, Genetics, QH426-470
Abstract

Abstract The genus Flavivirus comprises numerous emerging and re‐emerging arboviruses causing human illness. Vaccines are the best approach to prevent flavivirus diseases. But pathogen diversities are always one of the major hindrances for timely development of new vaccines when confronting unpredicted flavivirus outbreaks. We used West Nile virus (WNV) as a model to develop a new live‐attenuated vaccine (LAV), WNV‐poly(A), by replacing 5ʹ portion (corresponding to SL and DB domains in WNV) of 3ʹ‐UTR with internal poly(A) tract. WNV‐poly(A) not only propagated efficiently in Vero cells, but also was highly attenuated in mouse model. A single‐dose vaccination elicited robust and long‐lasting immune responses, conferring full protection against WNV challenge. Such “poly(A)” vaccine strategy may be promising for wide application in the development of flavivirus LAVs because of its general target regions in flaviviruses.

Published
2021
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3. Rational design of West Nile virus vaccine through large replacement of 3′ UTR with internal poly(A)

Author

Jing Liu, Zhiming Yuan, Ya-Nan Zhang, Yuan Shaopeng, Cheng-Lin Deng, Qiu-Yan Zhang, Xiao-Dan Li, Bo Zhang, Hong-Qing Zhang, Pei Yong Shi, Han-Qing Ye, Fang Yu, Zhan Shunli, Gao Lei, Na Li, and Xiang-Yue Zeng

Subjects
Untranslated region, Medicine (General), live‐attenuated vaccine, viruses, Immunology, QH426-470, Biology, Antibodies, Viral, Article, UTR, Mice, R5-920, Immune system, flavivirus, Chlorocebus aethiops, Genetics, West Nile Virus, Animals, West Nile Virus Vaccines, 3' Untranslated Regions, Vero Cells, Pathogen, internal poly(A), Attenuated vaccine, virus diseases, Outbreak, Articles, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, Virology, Microbiology, Virology & Host Pathogen Interaction, Vaccination, Flavivirus, Vero cell, Molecular Medicine, Poly A, West Nile Fever
Abstract

The genus Flavivirus comprises numerous emerging and re‐emerging arboviruses causing human illness. Vaccines are the best approach to prevent flavivirus diseases. But pathogen diversities are always one of the major hindrances for timely development of new vaccines when confronting unpredicted flavivirus outbreaks. We used West Nile virus (WNV) as a model to develop a new live‐attenuated vaccine (LAV), WNV‐poly(A), by replacing 5ʹ portion (corresponding to SL and DB domains in WNV) of 3ʹ‐UTR with internal poly(A) tract. WNV‐poly(A) not only propagated efficiently in Vero cells, but also was highly attenuated in mouse model. A single‐dose vaccination elicited robust and long‐lasting immune responses, conferring full protection against WNV challenge. Such “poly(A)” vaccine strategy may be promising for wide application in the development of flavivirus LAVs because of its general target regions in flaviviruses., West Nile virus (WNV) is one of the many members of the genus Flavivirus known to cause human diseases. Here we present a novel live attenuated WNV vaccine, WNV‐poly(A), that protects against WNV infections in a mouse model.

Published
2021
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4. Prognostic value of lymph node metastasis in patients with T1-stage colorectal cancer from multiple centers in China

Author

Ma Shuai, Zhiyong Zhang, Weiguo Ren, Quanbo Zhou, Jinbo Liu, Xiang Xie, Sun Xiantao, Junmin Song, Zhenqiang Sun, Zhen Li, Shuaixi Yang, Fan-Ye Zeng, Xiang-Yue Zeng, Weitang Yuan, Yuan Chang, Guixian Wang, and Fanghai Han

Subjects
Male, Oncology, China, medicine.medical_specialty, Colorectal cancer, Kaplan-Meier Estimate, Lymph node metastasis, T1 stage, 03 medical and health sciences, 0302 clinical medicine, Retrospective Study, Risk Factors, Internal medicine, Biomarkers, Tumor, medicine, Humans, In patient, Stage (cooking), Aged, Neoplasm Staging, Retrospective Studies, business.industry, Gastroenterology, General Medicine, Middle Aged, Prognosis, medicine.disease, Lymphatic Metastasis, 030220 oncology & carcinogenesis, Preoperative Period, Female, 030211 gastroenterology & hepatology, Lymph Nodes, Colorectal Neoplasms, business, Value (mathematics), Follow-Up Studies
Abstract

AIM To explore the features and prognostic value of lymph node metastasis in patients with T1-stage colorectal cancer (CRC). METHODS In all, 321 cases of T1-stage CRC were selected from 10132 patients with CRC who received surgical therapy in six large-scale hospitals in China and were retrospectively analyzed. Univariate and multivariate analyses were performed to analyze the risk factors for lymphatic metastasis. A survival analysis was then performed to analyze the prognostic value of lymph node metastasis. RESULTS The occurrence rate of T1 stage was 3.17% (321/10132); of these patients, the lymph node metastasis rate was 8.41% (27/321), and the non-lymph node metastasis rate was 91.59% (294/321). Univariate analysis showed that preoperative serum CEA, preoperative serum CA199, preoperative serum CA724, vascular invasion, and degree of differentiation were associated with lymph node metastasis in T1-stage CRC (P < 0.05 for all). Multivariate analysis indicated that preoperative serum CA724, vascular invasion, and degree of differentiation were closely related to lymph node metastasis (P < 0.05 for all). Log-rank survival analysis showed that age, preoperative serum CEA, preoperative serum CA199, vascular invasion, degree of differentiation, and lymph node metastasis (χ2 = 24.180, P < 0.001) were predictors of 5-year overall survival (OS) (P < 0.05 for all). COX regression analysis demonstrated that preoperative serum CA199 and lymph node metastasis (HR = 5.117; P < 0.05; 95%CI: 0.058-0.815) were independent prognostic indicators of 5-year OS in patients with T1-stage CRC (P < 0.05 for both). CONCLUSION The morbidity of T1-stage CRC was 3.17% for all CRC cases. Preoperative serum CA724, vascular invasion, and degree of differentiation are independent risk factors for lymph node metastasis. Lymph node metastasis is an independent prognostic factor for OS in patients with T1-stage CRC.

Published
2017
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5. MiR-590-3p promotes proliferation and metastasis of colorectal cancer via Hippo pathway

Author

Jinbo Liu, Qisan Wang, Shuaixi Yang, Quanbo Zhou, Zhen Li, Weitang Yuan, Guixian Wang, Ke Shi, Hai-Jiang Wang, Zhenqiang Sun, Xiang-Yue Zeng, and Junmin Song

Subjects
0301 basic medicine, MST1, Pathology, medicine.medical_specialty, Colorectal cancer, Hippo pathway, proliferation, colorectal cancer, Metastasis, 03 medical and health sciences, 0302 clinical medicine, medicine, metastasis, Luciferase, YAP1, Hippo signaling pathway, Oncogene, business.industry, Cell growth, miR-590-3p, medicine.disease, 030104 developmental biology, Oncology, 030220 oncology & carcinogenesis, Cancer research, business, Research Paper
Abstract

Studies reported that miR-590-3p was involved in human cancer progression. However, its roles of oncogene or anti-oncogene in malignancies still remain elusive. This study was aimed to investigate the effect of miR-590-3p on the cell proliferation and metastasis via Hippo pathway in colorectal cancer (CRC). In our study, miR-590-3p was demonstrated highly expressed in CRC tissues, compared with adjacent normal tissues (P

Published
2017

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