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Rational design of West Nile virus vaccine through large replacement of 3′ UTR with internal poly(A)
- Source :
- EMBO Molecular Medicine, EMBO Molecular Medicine, Vol 13, Iss 9, Pp n/a-n/a (2021)
- Publication Year :
- 2021
- Publisher :
- EMBO, 2021.
-
Abstract
- The genus Flavivirus comprises numerous emerging and re‐emerging arboviruses causing human illness. Vaccines are the best approach to prevent flavivirus diseases. But pathogen diversities are always one of the major hindrances for timely development of new vaccines when confronting unpredicted flavivirus outbreaks. We used West Nile virus (WNV) as a model to develop a new live‐attenuated vaccine (LAV), WNV‐poly(A), by replacing 5ʹ portion (corresponding to SL and DB domains in WNV) of 3ʹ‐UTR with internal poly(A) tract. WNV‐poly(A) not only propagated efficiently in Vero cells, but also was highly attenuated in mouse model. A single‐dose vaccination elicited robust and long‐lasting immune responses, conferring full protection against WNV challenge. Such “poly(A)” vaccine strategy may be promising for wide application in the development of flavivirus LAVs because of its general target regions in flaviviruses.<br />West Nile virus (WNV) is one of the many members of the genus Flavivirus known to cause human diseases. Here we present a novel live attenuated WNV vaccine, WNV‐poly(A), that protects against WNV infections in a mouse model.
- Subjects :
- Untranslated region
Medicine (General)
live‐attenuated vaccine
viruses
Immunology
QH426-470
Biology
Antibodies, Viral
Article
UTR
Mice
R5-920
Immune system
flavivirus
Chlorocebus aethiops
Genetics
West Nile Virus
Animals
West Nile Virus Vaccines
3' Untranslated Regions
Vero Cells
Pathogen
internal poly(A)
Attenuated vaccine
virus diseases
Outbreak
Articles
biochemical phenomena, metabolism, and nutrition
biology.organism_classification
Virology
Microbiology, Virology & Host Pathogen Interaction
Vaccination
Flavivirus
Vero cell
Molecular Medicine
Poly A
West Nile Fever
Subjects
Details
- ISSN :
- 17574684 and 17574676
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- EMBO Molecular Medicine
- Accession number :
- edsair.doi.dedup.....aefa3b412203aaad6a99608e9651ec03