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103 results on '"Padilla V"'

1. Occupancy Estimation at Bus Stops Through Wi-Fi Connectivity Assessment – A Study in Guayaquil City

Author: Cheme Viviana Mero, Martinez Martin Herrera, Durango Rayner, and Padilla V. Sanchez
Subjects: bus stops, design thinking, transport system, user counting, wi-fi assessment, wi-fi simulation, Transportation and communication, K4011-4343
Abstract: Guayaquil is one of the most congested cities on the American continent. Several users cannot access on-time transport service according to the demand requested at bus stops. This work presents a simulation of the Wi-Fi technology to determine the number of users present at a bus stop. The objective is to know an estimation of them in different time intervals throughout the day, which could be useful for several actions, e.g., to set up a better distribution of transportation schedules or to place the bus stops in more demanding sites by users. The methodology included simulation scenarios for user counting at the bus stops. The results depicted that Wi-Fi technology performed suitable even in high-level attenuation scenarios.
Published: 2022
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2. Lorawan-Based RSSI-Trilateration Model for Node Location: A Simulation Integrating Flora and Omnet++

Author: Yi Loor Jiawey D., Espinal Albert, and Sanchez Padilla V.
Subjects: framework for lora, low-power technologies, omnet++, path loss, rssi, Transportation and communication, K4011-4343
Abstract: This work presents the employing of LoRaWAN (Long Range Wide Area Network) for location applications through a network simulation to determine a mobile node position. We rely on FLoRa (Framework for LoRa) and OMNeT++ (Objective Modular Network Testbed in C++) simulator, which uses Python feature tools, following the calculation of node placement using the trilateration technique. Our method differs from others in that we calculate the FLoRa power loss and determine different simulation settings using the shadowing feature of the log-distance path loss model. We approached RSSI (Received Signal Strength Indicator) to measure the distance between the LoRa gateways and the nodes, establishing a link between these parameters. Our work aims to promote the integration of open-source tools for verifying signal intensity values based on node distance from gateways. We consider it useful for engineers in predicting signal behaviors according to topology and settings variations. During the experimentation, the network underwent different performances according to the transmission parameters considered during the simulation. This was critical when increasing the number of mobile nodes, leading to consuming computer capacity and resources. Through repetition of tests, we confirmed the lower intensity of the received signal as the node moves to farther positions, reaching consistent power indicators and positioning accuracy. Overall, the results show that LoRaWAN integrated with trilateration techniques can be practical in providing adequate performance for node positioning accuracy and long-distance communication with low power consumption.
Published: 2024
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3. Industry Members’ Perceptions About ABET-Based Accreditation: An Exploratory Study in a Developing Country

Author: Sanchez Padilla, V., Espinal, Albert, Case, Jennifer M., Cordova-Garcia, Jose, and Murzi, Homero
Abstract: Contribution: This study explores industry members’ perceptions about the ABET-based accreditation in a developing country, using the case study of a program in a publicly funded polytechnic university in Ecuador. Background: Engineering programs often seek international accreditations to enhance the education quality, align with the global standards or gain academic reputation. ABET-based accreditation originates in United States, and thus presents some challenges for institutions in developing countries. Intended Outcomes: This study aimed to investigate the significance of international academic accreditation, as seen through the perspective of industry members. It focused on identifying fundamental competencies valued by employers and aimed to provide insights for institutions in similar contexts. Application Design: The research employed an approach informed by a qualitative methodology, involving in-depth interviews with five industry members who had served on the advisory committee board of an engineering program from a higher education institution in Ecuador. Findings: The findings identified three main areas of competencies that the industry members value: communication skills for teamwork, a problem-solving orientation, and an ability for effective task planning. The study also showed that these industry members value international accreditation as a means for enhancing education quality and ensuring graduates develop the necessary skills and competencies, yet the overall awareness with their peers remains relatively low.
Published: 2024
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4. Susceptibility divergence, phase transition and multistability of a highly turbulent closed flow

Author: Cortet, P. -P., Herbert, E., Chiffaudel, A., Daviaud, F., Dubrulle, B., and Padilla, V.
Subjects: Condensed Matter - Statistical Mechanics, Physics - Fluid Dynamics
Abstract: Using time-series of stereoscopic particle image velocimetry data, we study the response of a turbulent von K\'{a}rm\'{a}n swirling flow to a continuous breaking of its forcing symmetry. Experiments are carried over a wide Reynolds number range, from laminar regime at $Re = 10^{2}$ to highly turbulent regime near $Re = 10^{6}$. We show that the flow symmetry can be quantitatively characterized by two scalars, the global angular momentum $I$ and the mixing layer altitude $z_s$, which are shown to be statistically equivalent. Furthermore, we report that the flow response to small forcing dissymetry is linear, with a slope depending on the Reynolds number: this response coefficient increases non monotonically from small to large Reynolds number and presents a divergence at a critical Reynolds number $Re_c = 40\,000 \pm 5\,000$. This divergence coincides with a change in the statistical properties of the instantaneous flow symmetry $I(t)$: its pdf changes from Gaussian to non-Gaussian with multiple maxima, revealing metastable non-symmetrical states. For symmetric forcing, a peak of fluctuations of $I(t)$ is also observed at $Re_c$: these fluctuations correspond to time-intermittencies between metastable states of the flow which, contrary to the very-long-time-averaged mean flow, spontaneously and dynamically break the system symmetry. We show that these observations can be interpreted in terms of divergence of the susceptibility to symmetry breaking, revealing the existence of a phase transition. An analogy with the ferromagnetic-paramagnetic transition in solid-state physics is presented and discussed., Comment: to appear in Journal of Statistical Mechanics
Published: 2011
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5. FIRST REPORT OF GRAPEVINE LEAFROLL-ASSOCIATED VIRUS 9 IN SPAIN

Author: Padilla, C.V., Cretazzo, E., Alcalà, M.J., Hita, I., López, N., Padilla, V., and Velasco, L.
Published: 2013

6. Inga colimana (Leguminosae) una especie nueva del occidente de México

Author: Eloy Padilla V., Ram\u00F3n Cuevas G., and Arturo Sol\u00EDs M.
Subjects: Botany, QK1-989
Abstract: Se ilustra y describe como especie nueva Inga colimana, conocida únicamente del estado de Colima. El nuevo taxon pertenece a la sección Leptinga y parece estar relacionado con I. jinicuil G. Don e I. cinnamomea Spruce ex Benth., de las cuales se diferencia por sus frutos y semillas más pequeños.
Published: 2005

7. Renewable energy sources in the Colombian energy policy, analysis and perspectives

Author: Ruiz, B.J. and Rodríguez-Padilla, V.
Published: 2006
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8. Environmental factors contributing to the development of childhood leukemia in children with Down's syndrome

Author: Mejía-Aranguré, J M, Fajardo-Gutiérrez, A, Flores-Aguilar, H, Martínez-García, M C, Salamanca-Gómez, F, Palma-Padilla, V, Paredes-Aguilera, R, Bernáldez-Ríos, R, Ortiz-Fernández, A, Martínez-Avalos, A, and Gorodezky, C
Published: 2003
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9. Detection of Grapevine virus B associated with rugose wood (corky bark) symptoms in grapevine cv. Napoleon in Murcia (Spain)

Author: Velasco, L., García, B., Hita, I., and Padilla, V.
Published: 2005

10. Modelización de tableros de obras de paso de luces cortas ejecutados con placa alveolar pretensada

Author: Moreno-Padilla, V. and Albajar-Molera, L.
Subjects: torsión, Puentes, torsion, juntas longitudinales, longitudinal joints, hollow core slab, placa alveolar, Bridges
Abstract: Traditional methods of plane grilled modeling used for the calculation of prestressed hollow core slab bridge decks not provide sufficient accuracy to determine the influence of phenomena such as slabs torsion or shear between the interface slab-toping. A new hybrid modeling approach is proposed. It allows more realistically quantify the resistant mechanism of this type of decks. The application of the method on a typical deck has yielded results that show the good correlation between the numerical predictions and the behavior observed in reality. Los métodos tradicionales de emparrillado plano utilizados para el cálculo de tableros conformados con placa alveolar pretensada no aportan suficiente precisión para determinar la influencia de fenómenos tales como la torsión sobre las placas o el rasante entre la capa de reparto y la propia placa. Se propone un nuevo método de modelización híbrido que permite cuantificar de forma más realista el mecanismo resistente de este tipo de tableros. La aplicación del método sobre un tablero tipo ha permitido obtener unos resultados que demuestran la buena correlación de las predicciones numéricas con el comportamiento observado en la realidad.
Published: 2014
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11. Experimental characterization of extreme events of inertial dissipation in a turbulent swirling flow

Author: Saw, E. -W., primary, Kuzzay, D., additional, Faranda, D., additional, Guittonneau, A., additional, Daviaud, F., additional, Wiertel-Gasquet, C., additional, Padilla, V., additional, and Dubrulle, B., additional
Published: 2016
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12. Incidence of virus infection in old vineyards of local grapevine varieties from Majorca: implications for clonal selection strategies

Author: Cretazzo, E., Tomás, M., Padilla, C., Roselló, J., Medrano, H., Padilla, V., and Cifre, J.
Subjects: autochthonous varieties, grapevine certification, standard material virus incidence, certificación en Vitis vinífera, incidencia de virosis, material estándar, variedades autóctonas
Abstract: Three autochthonous grapevine varieties of Majorca (Spain) were analyzed for the presence of viruses listed by the international certification programs. Enzyme-Linked Immuno-Sorbent Assay (ELISA) screenings were performed in 193 vines from 46 vineyards included in a clonal selection. Virus-free vines were only 6.4%, 9.6% and 11.5%, in Manto Negro, Callet and Moll, respectively. Infections by grapevine leafroll associated viruses (GLRaVs) were ascertained in 71%, 78% and 60% of Manto Negro, Callet and Moll vines, respectively. Each variety was also highly infected by Grapevine fanleaf virus (GFLV) and Grapevine fleck virus (GFkV). The percentage of plants displaying multiple infections was 58.4% in Manto Negro, 63.8% in Callet and 42.6% in Moll. Thus, it was very difficult to find virus-free clones with suitable agronomic characteristics to be considered as a reference for the grape market. In order to obtain certified propagation material under such conditions of endemic viral infection, sanitation should be the main focus in clonal selection processes. However, the time and financial requirements for proper sanitation process bring to consideration the need to use, at least temporarily, standard multiplication material while certified clones are achieved., En este estudio se analizó la presencia de las virosis contempladas por las leyes internacionales en tema de certificación en tres variedades de vid autóctonas de Mallorca. Para ello se realizó el test ELISA (enzyme-linked immuno-sorbent assay) sobre 193 cepas procedentes de 46 viñedos incluidos en un programa de selección clonal. Las cepas que resultaron libres de los virus analizados fueron el 6,4% en Manto Negro, el 9,6% en Callet y el 11,5% en Moll. Los porcentajes de cepas infectadas por los virus asociados al síndrome del enrollado de la vid (GLRaVs) fueron 71% en Manto Negro, 78% en Callet y 60% en Moll. Se detectaron también altas tasas de infecciones para el virus del entrenudo corto infeccioso de la vid (GFLV) y el virus del jaspeado de la vid (GFkV) en las tres variedades. Los porcentajes de cepas sujetas a infecciones múltiples fueron 58,4% en Manto Negro, 63,8% en Callet y 42,6% en Moll. Por tanto fue difícil encontrar cepas que cumpliesen tanto los requisitos sanitarios para la certificación como aquellos agronómicos necesarios según las exigencias del mercado vitivinícola. En tales condiciones de infecciones víricas endémicas, el saneamiento debería ser el primer objetivo a seguir con el fin de obtener material de propagación certificado. Sin embargo, el tiempo y los recursos económicos necesarios para llevar a cabo el proceso de saneamiento conllevan la necesidad de considerar el uso temporal de material de propagación estándar hasta que se obtengan clones certificados.
Published: 2010

13. Impacto en salud infantil del invierno 2002: disociación entre factores ambientales y virus respiratorio sincicial, en Santiago

Author: Avendaño C, Luis F, Parra V, Jorge, Padilla V, Carolina, and Palomino M, María Angélica
Subjects: Health care costs, Environmental exposure, Respiratory Syncytial virus infections
Abstract: The increase in winter related health pediatric demand is associated with three factors: cold and rainy weather, air pollution and respiratory viral epidemics. During the winter of 2002 there was, successively, heavy rain, air pollution, cold weather and a respiratory syncytial virus (RSV) epidemic in Santiago. Aim: To study the influence of environmental factors and RSV epidemic on pediatric health care demand. Patients and methods: The number of hospital admissions and outpatient consultations for acute lower respiratory infections (ALRI) from April to October 2002 (Autumn to Spring in Chile), were registered in a public pediatric hospital of Santiago. A respiratory virus surveillance (RSV, adenovirus influenza and parainfluenza virus) was carried out among children admitted for ALRI. Climate conditions and air pollution (number of particles of more than 10 microns/mm3) values were provided by local environmental health services. Results: As expected, a rise in winter hospital admissions and outpatient consultations was detected, that peaked in week 29. This rise coincided with the higher RSV detection week. There was heavy rain in weeks 22, 23 and 30, as well as cold weather in June-July (weeks 23, 24, 26, 27 and 30; mid Winter), that did not increase health care demand. Likewise, high air pollution, registered from weeks 24 to 26, did not increase health care demand. Conclusions: RSV epidemic is the principal factor associated to the increase in health burden during winter in Santiago (Rev Méd Chile 2003; 131: 902-8)
Published: 2003

14. Impacto en salud infantil del invierno 2002: disociación entre factores ambientales y virus respiratorio sincicial, en Santiago

Author: Avendaño C,Luis F, Parra V,Jorge, Padilla V,Carolina, and Palomino M,María Angélica
Subjects: Health care costs, Environmental exposure, Respiratory Syncytial virus infections
Abstract: The increase in winter related health pediatric demand is associated with three factors: cold and rainy weather, air pollution and respiratory viral epidemics. During the winter of 2002 there was, successively, heavy rain, air pollution, cold weather and a respiratory syncytial virus (RSV) epidemic in Santiago. Aim: To study the influence of environmental factors and RSV epidemic on pediatric health care demand. Patients and methods: The number of hospital admissions and outpatient consultations for acute lower respiratory infections (ALRI) from April to October 2002 (Autumn to Spring in Chile), were registered in a public pediatric hospital of Santiago. A respiratory virus surveillance (RSV, adenovirus influenza and parainfluenza virus) was carried out among children admitted for ALRI. Climate conditions and air pollution (number of particles of more than 10 microns/mm3) values were provided by local environmental health services. Results: As expected, a rise in winter hospital admissions and outpatient consultations was detected, that peaked in week 29. This rise coincided with the higher RSV detection week. There was heavy rain in weeks 22, 23 and 30, as well as cold weather in June-July (weeks 23, 24, 26, 27 and 30; mid Winter), that did not increase health care demand. Likewise, high air pollution, registered from weeks 24 to 26, did not increase health care demand. Conclusions: RSV epidemic is the principal factor associated to the increase in health burden during winter in Santiago (Rev Méd Chile 2003; 131: 902-8)
Published: 2003

15. Incidence of virus infection in old vineyards of local grapevine varieties from Majorca: implications for clonal selection strategies

Author: Cretazzo, Enrico, Tomás Mir, Magdalena, Padilla, C., Cifre Llompart, Josep, Rosselló Veny, Joan, Medrano Gil, Hipólito, Padilla, V., Cretazzo, Enrico, Tomás Mir, Magdalena, Padilla, C., Cifre Llompart, Josep, Rosselló Veny, Joan, Medrano Gil, Hipólito, and Padilla, V.
Abstract: Three autochthonous grapevine varieties of Majorca (Spain) were analyzed for the presence of viruses listed by the international certification programs. Enzyme-Linked Immuno-Sorbent Assay (ELISA) screenings were performed in 193 vines from 46 vineyards included in a clonal selection. Virus-free vines were only 6.4%, 9.6% and 11.5%, in Manto Negro, Callet and Moll, respectively. Infections by grapevine leafroll associated viruses (GLRaVs) were ascertained in 71%, 78% and 60% of Manto Negro, Callet and Moll vines, respectively. Each variety was also highly infected by Grapevine fanleaf virus (GFLV) and Grapevine fleck virus (GFkV). The percentage of plants displaying multiple infections was 58.4% in Manto Negro, 63.8% in Callet and 42.6% in Moll. Thus, it was very difficult to identify virusfree clones with suitable agronomic characteristics to be considered as a reference for the grape market. In order to obtain certified propagation material under such conditions of endemic viral infection, sanitation should be the main focus in clonal selection processes. However, the time and financial requirements for proper sanitation process bring to consideration the need to use, at least temporarily, standard multiplication material while certified clones are achieved., En este estudio se analizó la presencia de las virosis contempladas por las leyes internacionales en tema de certificación en tres variedades de vid autóctonas de Mallorca. Para ello se realizó el test ELISA (enzyme-linked immunosorbent assay) sobre 193 cepas procedentes de 46 viñedos incluidos en un programa de selección clonal. Las cepas que resultaron libres de los virus analizados fueron el 6,4% en Manto Negro, el 9,6% en Callet y el 11,5% en Moll. Los porcentajes de cepas infectadas por los virus asociados al síndrome del enrollado de la vid (GLRaVs) fueron 71% en Manto Negro, 78% en Callet y 60% en Moll. Se detectaron también altas tasas de infecciones para el virus del entrenudo corto infeccioso de la vid (GFLV) y el virus del jaspeado de la vid (GFkV) en las tres variedades. Los porcentajes de cepas sujetas a infecciones múltiples fueron 58,4% en Manto Negro, 63,8% en Callet y 42,6% en Moll. Por tanto fue difícil encontrar cepas que cumpliesen tanto los requisitos sanitarios para la certificación como aquellos agronómicos necesarios según las exigencias del mercado vitivinícola. En tales condiciones de infecciones víricas endémicas, el saneamiento debería ser el primer objetivo a seguir con el fin de obtener material de propagación certificado. Sin embargo, el tiempo y los recursos económicos necesarios para llevar a cabo el proceso de saneamiento conllevan la necesidad de considerar el uso temporal de material de propagación estándar hasta que se obtengan clones certificados.
Published: 2010

16. Case report of an adolescent girl with Kabuki syndrome and kyphoscoliosis, resistant at the conservative orthopedic treatment

Author: Taranu, MA, primary, Giralt, M Colomer, additional, Padilla, V Calderón, additional, Blaya, V Pujol, additional, Morán, L Quesada, additional, Walker, JM Cavanilles, additional, García, BM Núñez, additional, and Monje, C Rodríguez, additional
Published: 2012
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17. Síndrome de microdeleción 1p36

Author: Ortigosa Gómez, S., primary, Seidel Padilla, V., additional, Cuscó, I., additional, and Aznar Lain, G., additional
Published: 2011
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18. First Report of Grapevine leafroll-associated virus 5 in Spain

Author: Padilla, C. V., primary, Cretazzo, E., additional, Hita, I., additional, López, N., additional, Padilla, V., additional, and Velasco, L., additional
Published: 2010
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19. First Report of Arabis mosaic virus on Grapevine in Spain

Author: Abelleira, A., primary, Mansilla, J. P., additional, Padilla, V., additional, Hita, I., additional, Cabaleiro, C., additional, Bertolini, E., additional, Olmos, A., additional, and Legorburu, F. J., additional
Published: 2010
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20. First report of Grapevine leafroll‐associated virus 4 (GLRaV‐4) in Spain

Author: Padilla, C.V., primary, Cretazzo, E., additional, López, N., additional, Rosa, B. García de, additional, Padilla, V., additional, and Velasco, L., additional
Published: 2010
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21. Variz venosa umbilical intramniótica. Reporte de un caso.

Author: Soriano-Lillo, Paula, Padilla-V, Carmen, Blázquez-R, Ana Rosa, and Crespo, V
Subjects: UMBILICAL veins, VARICOSE veins, PRENATAL diagnosis, FETAL monitoring, FETAL abnormalities, DISEASES, DIAGNOSIS
Abstract: Copyright of Ginecología y Obstetricia de México is the property of Federacion Mexicana de Ginecologia y Obstetricia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Published: 2015

22. Inga colimana (Leguminosae) una especie nueva del occidente de México

Author: Padilla V., Eloy, primary, Cuevas G., Ramón, additional, and Solís M., Arturo, additional
Published: 2005
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23. La educación universitaria ecuatoriana: crisis y expectativas para el siglo XXI

Author: Córdova Alvarez, Pedro, Padilla V., Fabricio, Paida V., Arturo, Córdova Alvarez, Pedro, Padilla V., Fabricio, and Paida V., Arturo
Published: 1996

24. Campaign for pesticide free rice

Author: Garcia - Padilla, V., Padilla, H., Garcia - Padilla, V., and Padilla, H.
Abstract: The LEISA-Rice Production Campaign launched by AGTALON in April 1992 in Pangasinan, Philippines is described and some elements and initial results are given, based on data collected from 84 cooperating farmers
Published: 1993

25. Esquemas de ordenamiento urbano parroquial de Guachapala, El Pan, Sevilla de Oro y Palmas

Author: Cordero Domínguez, Oswaldo, Abad G., Juan, Avila I., Milton, Olmedo A., Jaqueline, Padilla V., Julio, Cordero Domínguez, Oswaldo, Abad G., Juan, Avila I., Milton, Olmedo A., Jaqueline, and Padilla V., Julio
Published: 1991

26. Síndrome de microdeleción 1p36.

Author: Ortigoza Gómez, S., Seidel Padilla, V., Cuscó, I., Aznar Lain, G., Rodríguez Ogando, A., Roldán Martín, M. B., Arnao, Rodríguez, Rodríguez Sánchez, A., González López, M. T., Corral Caramés, M. J., Fernández Cebrián, S., Martinón Sánchez, F., Coucea, M. L., Moure, J., Pérez-Muñuzuri, A., Aldamiz-Echevarría, L., Fraga, J. M., Delgado Luengo, W., Fleitas Cabello, H., and Solís Áñez, E.
Published: 2011
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27. Piper amalago

Author: Paul C. Standley & Elias Padilla V., Paul C. Standley & Elias Padilla V., Paul C. Standley & Elias Padilla V., and Paul C. Standley & Elias Padilla V.
Abstract: Angiosperms, http://name.umdl.umich.edu/IC-HERB00IC-X-1641054%5DMICH-V-1641054, https://quod.lib.umich.edu/cgi/i/image/api/thumb/herb00ic/1641054/MICH-V-1641054/!250,250, The University of Michigan Library provides access to these materials for educational and research purposes. Some materials may be protected by copyright. If you decide to use any of these materials, you are responsible for making your own legal assessment and securing any necessary permission. If you have questions about the collection, please contact the Herbarium professional staff: herb-dlps-help@umich.edu. If you have concerns about the inclusion of an item in this collection, please contact Library Information Technology: libraryit-info@umich.edu., https://www.lib.umich.edu/about-us/policies/copyright-policy
Published: 1947

28. ANÁLISIS DEL TIPO DE INTERACCIONES QUE OCURREN ENTRE INVESTIGADORES DURANTE LA PRESENTACIÓN DE TRABAJOS ACADÉMICOS

Author: Padilla Vargas, María Antonia
Subjects: SCIENTIFIC PROGRESS, SCIENTISTS, INTERACTIONS, STATUS, DOMINANCE, Psychology, BF1-990
Abstract: Although it is considered that scientific development and intellectual progress occur through the exchange of ideas brought about by the interaction among scientists, the type of interactions that take place among researchers when one of them presents their work to colleagues has not been studied systematically. Given the above, a preliminary study whose objective was to analyze the interactions among researchers from the same university working on different areas was conducted to identify the number of comments made, the academic status of the one making the comment, the particular aspect about which the comment was made and the outcome (agreement or disagreement). Eight tenured professors, five associate professors, 16 graduate students, and four undergraduate scholarship holders took part in the discussions. The interactions that occurred during 13 work presentations were recorded, transcribed and analyzed. Results showed that: a) the lower the academic status of the presenter, the greater the number of comments made about their work; b) those with a higher academic status intervened more; c) both theoretical and methodological comments occurred, but the former occurred slightly more often than the latter; and d) a greater difference in status between the presenter and the one making the comment resulted in more agreements. The way the status mediates the interactions among research groups and the dominance relations in scientific practice are discussed.
Published: 2014
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29. Bolted Field Repair of Composite Structures.

Author: MCDONNELL AIRCRAFT CO ST LOUIS MO, Watson,James B, Glaeser,D A, Harvey,F L, Fukimoto,W T, Padilla,V E, MCDONNELL AIRCRAFT CO ST LOUIS MO, Watson,James B, Glaeser,D A, Harvey,F L, Fukimoto,W T, and Padilla,V E
Abstract: Mechanically fastened, field repairs for graphite/epoxy laminates 3/16 and 1/2 inch thick with through-the-thickness hole damage have been successfully demonstrated. These repairs (titanium alloy patches and backing plates), were developed for application on fuel cell composite wing surfaces and can be installed by maintenance personnel in the field using available equipment, materials, and methods. A total of 28 specimens were fabricated and tested statically to evaluate effectiveness of selected repair designs.
Published: 1979

30. Mortalidad infantíl hospitalaria en la provincia del Azuay, año 1978

Author: Barros Chica, Arturo José, Pino Rojas, Raúl, Pinos Narváez, Segundo, Padilla V., José, Rodas Pesántez, William, Pino Mora, Hernán, Barros Chica, Arturo José, Pino Rojas, Raúl, Pinos Narváez, Segundo, Padilla V., José, Rodas Pesántez, William, and Pino Mora, Hernán
Published: 1981

31. GNC challenges and opportunities of CUBESAT science missions deployed from the lunar gateway

Author: Kalita, H., Donayre, M., Padilla, V., Riley, A., Samitas, J., Burnett, B., Asphaug, E., Robinson, M., and Jekan Thangavelautham
Subjects: FOS: Physical sciences, Astrophysics - Instrumentation and Methods for Astrophysics, Instrumentation and Methods for Astrophysics (astro-ph.IM)
Abstract: The Lunar Gateway is expected to be positioned on-orbit around the Moon or in a Halo orbit at the L2 Lagrange point. The proposed Lunar Gateway is a game-changer for enabling new, high-priority lunar science utilizing Cu-beSats and presents a refreshing new opportunity for utilization of these small spacecraft as explorers. In context, CubeSats are being stretched to their limits as interplanetary explorers. The main technological hurdles include high-bandwidth communications and reliable high delta-v propulsion. Advances in deep-space attitude determination and control has been made possible from the recent NASA JPL MarCO missions. Due to these limitations, CubeSats are primarily designed to be dropped-off from a larger mission. The limited mass and volume have required compromises of the onboard science instruments, longer wait times to send back science data to Earth, shorter mission durations or higher accepted risk. With the Lunar Gateway being planned to be closer to the Moon, it will provide significant savings for a propulsion system and provide a primary relay for communication apart from the DSN and enable tele-operated command/control. These three factors can simplify the mission enabling routine deployment of CubeSats into lunar orbit and enable surface missions. In this paper, we present preliminary designs of 2 CubeSat lunar landers that will explore the lunar pits, Mare Tranquilitatis and the remnant magnetic fields Reiner Gamma., Comment: 13 pages, 12 figures, AAS GNC Conference 2019

32. Epidemiological and some clinical characteristics of neuroblastoma in Mexican children (1996-2005).

Author: Juárez-Ocaña S, Palma-Padilla V, González-Miranda G, Siordia-Reyes AG, López-Aguilar E, Aguilar-Martínez M, Mejía-Aranguré JM, Carreón-Cruz R, Rendón-Macías ME, Fajardo-Gutiérrez A, Juárez-Ocaña, Servando, Palma-Padilla, Virginia, González-Miranda, Guadalupe, Siordia-Reyes, Alicia Georgina, López-Aguilar, Enrique, Aguilar-Martínez, Martha, Mejía-Aranguré, Juan Manuel, Carreón-Cruz, Rogelio, Rendón-Macías, Mario Enrique, and Fajardo-Gutiérrez, Arturo
Abstract: Background: Neuroblastoma (NB) is the principal tumor of the sympathetic nervous system in children under one year of age. The incidence in developed countries is greater than that in developing countries. The aim of this article is to present the epidemiological and some clinical characteristics of Mexican children with NB.Methods: A population-based, prolective study, with data obtained from the Childhood Cancer Registry of the Instituto Mexicano de Seguro Social.Statistical Analysis: The simple frequencies of the variables of the study and the annual average incidence (per 1,000,000 children/years) by age and sex were obtained. The trend was evaluated by calculating the annual percentage of change. The curves of Kaplan-Meyer were employed for the survival rate and the log-rank test was used to compare the curves.Results: Of a total of 2,758 children with cancer registered during the period from 1996-2005, 72 (2.6%) were identified as having Group IV, defined according to the International Classification of Childhood Cancer. The incidence for NB was 3.8 per 1,000,000 children/year; NB was highest in the group of children under one year of age, followed by the group of children between the ages 1-4 years (18.5 and 5.4 per 1,000,000 children/years, respectively). The male/female ratio was 1.1 and there was no trend toward an increase. The time of diagnosis was 26 days (median), but varied according to the stage at diagnosis. Stages III and IV were presented in 88% of the cases. There was no association between the stage, the age at time of diagnosis, or the histological pattern. The overall five-year survival rate was 64%; the patients with stage I, II, III, or IVs did not die; and the five-year survival rate of cases in Stage IV was 40%.Conclusion: It is possible that the low incidence of neuroblastoma in Mexican children is due to the difficulty in diagnosing the cases with the best prognosis, some of which could have had spontaneous regression. There was no trend to an increase; the majority of the cases were diagnosed in the advanced stages; and the overall five-years survival rate was similar to that for developed countries. [ABSTRACT FROM AUTHOR]
Published: 2009
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33. Detection ofGrapevine virus Bassociated with rugose wood (corky bark) symptoms in grapevine cv. Napoleon in Murcia (Spain).

Author: Velasco, L., García, B., Hita, I., and Padilla, V.
Subjects: PLANT diseases, PETIOLES, LEAVES, PHOSPHATES, NICOTIANA, DOGWOODS
Abstract: The article focuses on the detection of Grapevine virus B associated with rugose wood symptoms in grapevine. The causal agent of the rugose wood disease complex, which includes corky bark, has been attributed to members of the Vitivirus genus, such as Grapevine virus A and Grapevine virus B. Petiole extracts of two positive plants with symptoms, in phosphate buffer containing 2.5% nicotinic acid, were used as sources for the mechanical inoculation of Nicotiana benthamiana and N. clevelandii plants.
Published: 2005
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34. Oil taxation and risks

Author: Rodriguez-Padilla, V [INRS-Energie, Varennes, Quebec (Canada)]
Published: 1992

35. 40(th) EASD Annual Meeting of the European Association for the Study of Diabetes : Munich, Germany, 5-9 September 2004

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Butler, Francisco Javier Ampudia-Blasco, O N Kong, Attali J-R., C A Hedman, K Oshinyemi, Nicolle Müller, I C Cranston, N Okumus, M V Vlaiculescu, Balasubramanian Ravikumar, W W Cheatham, K Mukasa, K B Biswas, Annunziata Lapolla, Phil McEwan, G Mader, Gilles Chassot, Dragi Anevski, Werner A. Scherbaum, M Donath, C Hesselmann, R A Gandhi, David E. Moller, Ezio Bonifacio, C Garcia, V Ifandi, P Hornnes, Nieuwenhoven Fav., C Puech, S Pérez-Del-Pulgar, Kim S-R., G Hines, C Rubio Terrés, Michael Gaster, N. Hosszufalusi, A Scholze, Andrew A. Young, Stavros Liatis, F Hariri, S Tan, Paul Valensi, Allan E. Karlsen, J Kim, E. Moberg, J Kaiser, L Berman, G Nelson, A Altkrüger, P Kothare, D B Cook, S Doran, G. van Dijk, Shahnaz Shahinfar, Kim C-S., P Stahl, M Manousaki, S Sigrist, S K Lim, M. P. Stern, A Guberti, C Rezzani, J McKenney, Karl Thomaseth, Sofia Carlsson, M Julia, R Brillante, I Rubesova, T Darkow, E Matsumoto, Wendy M. 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L, Villela, N, Laflor, C, Conde, C, Bottino, D, Dorigo, D, Bouskela, E, Pu, S, Luo, Z, Lam, K, Dan, Q, Xu, A, Shen, J, Cheng, K, Xu, J, Thamer, C, Stefan, N, Haap, M, Heller, E, Tschritter, O, De Prado, A, Ortiz, A, Ybarra, J, Gich, I, Pou, J, Ehren, M, Roggenland, D, Reinsen, B, Klein, H, Rittig, K, Stock, J, Kocher, B, Balletshofer, B, Shon, H, Chung, D, Nakatani, Y, Matsuhisa, M, Kaneto, H, Hatazaki, M, Yoshiuchi, K, Katakami, N, Kawamori, D, Ohtoshi, K, Sakamoto, K, Matsuoka, T, Ozawa, K, Ogawa, S, Hori, M, Yamasaki, Y, Zitouni, K, Harry, D, Nourooz zadeh, J, Earle, K, Olesen, P, Franco, L, Corvaja, C, Semplicini, A, Ceylan işık, A, Arı, N, Rösen, P, Lee, I, Park, K, Jung, E, Shin, D, Jo, S, Obuobie, K, Prakash, P, Hanna, F, Lazarus, J, Varadhan, L, Gurushankar, J, James, D, Sheikh, S, Gaede, P, Zou, D, Vilarrasa, N, Perez maraver, M, Mena, E, Perez, D, Setti, G, Buckingham, R, Urbančič, V, Stefanovska, A, Bernjak, A, Ažman juvan, K, Kocijančič, A, Glowania, A, Filters, T, Fosmark, D, Torjesen, P, Kilhovd, B, Berg, T, Sandvik, L, Hanssen, K, Mentink, C, Donchenko, G, Stepanenko, S, Maingrette, F, Deng, H, Lindenmair, A, Freudenthaler, A, Baumgartner parzer, S, Nizheradze, K, Khoruzhenko, A, Tronko, N, Sheu, W, Ou, H, Shen, H, Lin, T, Wu, H, Yang, C, Mogylnytska, L, Schmoelzer, I, Davies, J, Band, M, Struthers, A, Prázný, M, Škrha, J, Kasalová, Z, Neelotpol, S, Jahan, P, Kauschke, S, Harrop, C, Schäfer, A, Widder, J, Eigenthaler, M, Walter, U, Uchimura, I, Ikebukuro, M, Kaibara, M, Hirata, M, Helal, R, Pervin, F, Yang, X, Jansson, P, Nagaev, I, Jack, M, Carvalho, E, Sunnerhagen, K, Cam, M, Cushman, S, Smith, U, Creely, S, Farmer, J, Gustafson, B, Kusminski, C, Krusinova, E, Wohl, P, Klementova, M, Lanska, V, Mcdougall, C, Kelly, I, Abbas, Z, Lutale, J, Archibald, L, Karunajeewa, H, Stingemore, N, Stuccio, G, Mcgechie, D, Muller, L, Hak, E, Goudzwaard, W, Montorsi, F, Homering, M, Sprenger, K, Goldstein, I, Asnaghi, V, Ferrari, G, Rastaldi, M, Gabellini, D, Dell'Antonio, G, Maestroni, A, Ruggieri, D, Luzi, L, Piemonti, L, Zerbini, G, Anafaroglu, I, Tutuncu, N, Sultana, M, Siddiqua, N, Iwasaki, T, Nakajima, A, Yoneda, M, Mukasa, K, Tanaka, S, and Sekihara, H
Subjects: 0303 health sciences, medicine.medical_specialty, business.industry, EASD, Endocrinology, Diabetes and Metabolism, Human physiology, medicine.disease, 03 medical and health sciences, 0302 clinical medicine, Diabetes mellitus, Family medicine, Internal Medicine, Medicine, business, 030217 neurology & neurosurgery, 030304 developmental biology
Published: 2004

36. Transcriptomic dataset of the development and maturation of the Rhipicephalus microplus ovary.

Author: Cossío-Bayúgar R, Miranda-Miranda E, Aguilar-Díaz H, Narváez-Padilla V, and Reynaud E
Abstract: To conduct differential gene expression analysis, ovaries from the cattle tick Rhipicephalus microplus were dissected at three distinct developmental stages (preingurgitated, immature ingurgitated, and mature ingurgitated). Additionally, undissected intact mature males and complete ingurgitated female ticks without ovaries (carcasses) were also collected to serve as reference samples for analysis. To perform total RNA purification, tissue from ten individuals representing each of the five previously described conditions was pooled. mRNA was isolated from the purified total RNA using the oligo (dT) method. Following fragmentation, double stranded cDNA was synthesized and ligated to sequencing adapters. Suitable-sized fragments were subsequently used for PCR amplification. Libraries were analyzed and quantified using an Agilent 2100 Bioanalyzer and an ABI StepOnePlus Real-Time PCR System. A total of 45.64 Gb bases were sequenced using the Illumina HiSeq sequencing platform. After assembling the samples and correcting for abundance, we obtained 82,877 unigenes. The total length, average length, N50, and GC content of the unigenes were 89,754,828 bp,1,082 bp,2,068 bp and 49.04 % respectively. For functional annotation, the unigenes were aligned with 7 functional databases. The number of unigenes identified in the functional databases were as follows: 32,518 (NR:39.24 %), 10,259 (NT:12.38 %), 23,624 (Swissprot:28.50 %), 22,203 (KOG:26.79 %), 25,072 (KEGG:30.25 %), 17,435(GO:21.04 %), and 23,220 (InterPro:28.02 %). Unigene candidate coding regions (CDS) among the unigenes were predicted using TransDecoder software and 42,143 CDS were detected. We also detected 10,522 simple sequence repeats (SSRs) distributed on 8,126 unigenes, and predicted 4,672 transcription factors (TF) coding unigenes. Our data can be used to identify genes that are important for male and female tick and arachnid reproduction and tick general physiology., (© 2024 The Author(s).)
Published: 2024
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37. Optimizing SH-SY5Y cell culture: exploring the beneficial effects of an alternative media supplement on cell proliferation and viability.

Author: Kaya ZB, Santiago-Padilla V, Lim M, Boschen SL, Atilla P, and McLean PJ
Subjects: Humans, Cell Culture Techniques, Cell Line, Cell Differentiation, Cell Proliferation, Culture Media pharmacology, Neuroblastoma metabolism
Abstract: In the quest to unravel the mysteries of neurological diseases, comprehending the underlying mechanisms is supreme. The SH-SY5Y human neuroblastoma cell line serves as a crucial tool in this endeavor; however, the cells are known for its sensitivity and slow proliferation rates. Typically, this cell line is cultured with 10% Fetal Bovine Serum (FBS) supplement. Nu-Serum (NuS), a low-protein alternative to FBS, is promising to advance cell culture practices. Herein, we evaluated the substitution of NuS for FBS to test the hypothesis that an alternative serum supplement can aid and promote SH-SY5Y cell proliferation and differentiation. Our findings revealed that the NuS-supplemented group exhibited a notable increase in adhered cells compared to both the FBS and serum-free (SF) groups. Importantly, cell viability remained high in both sera treated groups, with the NuS-supplemented cells displaying significantly larger cell sizes compared to the SF-treated group. Furthermore, cell proliferation rates were higher in the NuS-treated group, and neuroblast-like morphology was observed earlier than FBS group. Notably, both FBS and NuS supported the differentiation of these cells into mature neurons. Our data supports NuS as an alternative for SH-SY5Y cell culture, with the potential to elevate the quality of research in the neuroscience field., (© 2024. The Author(s).)
Published: 2024
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38. Stigma toward substance use disorders: a multinational perspective and call for action.

Author: El Hayek S, Foad W, de Filippis R, Ghosh A, Koukach N, Mahgoub Mohammed Khier A, Pant SB, Padilla V, Ramalho R, Tolba H, and Shalbafan M
Abstract: Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision.
Published: 2024
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39. Thermal Transport and Rheological Properties of Hybrid Nanofluids Based on Vegetable Lubricants.

Author: Ribeiro H, Taha-Tijerina JJ, Gomez O, Acosta E, Pinto GM, Moraes LRC, Fechine GJM, Andrade RJE, Reinoza J, Padilla V, and Lozano K
Abstract: Nanofluids based on vegetal oil with different wt.% of carbon nanotubes (CNT), hexagonal boron nitride (h-BN), and its hybrid (h-BN@CNT) were produced to investigate the effects of these nano-additives on the thermal conductivity and rheological properties of nanofluids. Stable suspensions of these oil/nanostructures were produced without the use of stabilizing agents. The dispersed nanostructures were investigated by SEM, EDS, XRD, and XPS, while the thermal conductivity and rheological characteristics were studied by a transient hot-wire method and steady-state flow tests, respectively. Increases in thermal conductivity of up to 39% were observed for fluids produced with 0.5 wt.% of the hybrid nanomaterials. As for the rheological properties, it was verified that both the base fluid and the h-BN suspensions exhibited Newtonian behavior, while the presence of CNT modified this tendency. This change in behavior is attributed to the hydrophobic character of both CNT and the base oil, while h-BN nanostructures have lip-lip "bonds", giving it a partial ionic character. However, the combination of these nanostructures was fundamental for the synergistic effect on the increase of thermal conductivity with respect to their counterparts.
Published: 2023
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40. APOE Gene Associated with Dementia-Related Traits, Depression, and Anxiety in the Hispanic Population.

Author: Xu C, Padilla V, Lozano S, Gamez D, Su BB, Wang X, Maestre G, and Wang K
Subjects: Humans, Anxiety genetics, Depression genetics, Phenotype, Hispanic or Latino genetics, Alzheimer Disease genetics, Alzheimer Disease epidemiology, Apolipoproteins E genetics
Abstract: Alzheimer's disease (AD), a main cause of dementia, is commonly seen in aging populations with a strong genetic component. AD is one of the most common neurodegenerative disorders; it is a genetically and clinically heterogeneous disease. Specific demographic factors and genetic variants have been identified in non-Hispanic populations; however, limited studies have observed the Hispanic population. Therefore, we focused on investigating a known gene, APOE, associated with AD-related phenotypes and two psychiatric diseases (depression and anxiety) within the U.S. Hispanic population in our current study. A total of 1382 subjects were studied based on data collected from the Texas Alzheimer's Research and Care Consortium (TARCC, N = 1320) and the Initial Study of Longevity and Dementia from the Rio Grande Valley (ISLD-RGV, N = 62). Questionnaires regarding demographics, medical history, and blood/saliva samples were collected. We genotyped the APOE gene. The current findings indicated that APOE-ε4 was associated with not only AD ( p < 0.0001) but also with anxiety ( p < 0.0001) and depression ( p = 0.0004). However, APOE-ε3 was associated with depression ( p = 0.002) in the Hispanic population. We provide additional evidence in which APOE-ε4 increased the risk for AD in Hispanics. For the first time, APOE alleles show increased risks for anxiety and depression in Hispanics. Further research is warranted to confirm the current findings.
Published: 2023
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41. Sleep Apnea and Substance Use Disorders Associated with Co-Occurrence of Anxiety Disorder and Depression among U.S. Adults: Findings from the NSDUH 2008-2014.

Author: Xu C, Acevedo P, Wang L, Wang N, Ozuna K, Shafique S, Karithara A, Padilla V, Mao C, Xie X, and Wang K
Abstract: Few studies have focused on sleep apnea and substance use disorders with co-occurrence of anxiety disorder and depression. This study included a total of 270,227 adults, 9268 with co-occurrence of anxiety disorder and depression in the past year, from the combined 2008-2014 National Survey on Drug Use and Health (NSDUH) data, which are the latest datasets with measures of anxiety disorder and sleep apnea. Weighted multinomial logistic regression analyses were used to estimate the associations between anxiety disorder and depression and their co-occurrence. Comorbidity was highly prevalent: 40.4% of those with depression also met the criteria for anxiety disorder, whereas 51.8% of those with anxiety disorder also met the criteria for depression. The prevalences of anxiety only and co-occurrence increased from 2008 to 2014. The prevalences of anxiety disorder only, depression only, and co-occurrence of anxiety disorder and depression in individuals with sleep apnea were 4.4%, 12.9%, and 12.2%, respectively, and the prevalences in substance use disorders were 6.4%, 9.4%, and 10.7%, respectively. The results showed that sleep apnea, substance use disorders, and nicotine dependence were significantly associated with increased odds of anxiety disorder, depression, and co-occurrence (all p values < 0.0001). Furthermore, several chronic diseases (asthma, bronchitis, hypertension, and heart disease) were associated with the co-occurrence of anxiety disorder and depression. These findings suggest clinicians and other healthcare providers consider screening for depression and anxiety with sleep apnea and substance use disorders for improved therapeutic outcomes.
Published: 2023
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42. Parkinsonian phenotypes induced by Synphilin-1 expression are differentially contributed by serotonergic and dopaminergic circuits and suppressed by nicotine treatment.

Author: Carvajal-Oliveros A, Dominguez-Baleón C, Sánchez-Díaz I, Zambrano-Tipan D, Hernández-Vargas R, Campusano JM, Narváez-Padilla V, and Reynaud E
Subjects: Animals, Humans, Dopamine, Dopaminergic Neurons, Drosophila melanogaster, Phenotype, Nicotine pharmacology, Parkinsonian Disorders genetics, Nerve Tissue Proteins genetics
Abstract: Synphilin-1 is a protein encoded by the human SNCAIP gene whose function has yet to be fully understood. However, it has been linked to familial Parkinson's disease (PD). Synphilin-1 is a major component of the Lewy bodies found in neurons in the substantia nigra pars compacta of PD patients. Synphilin-1 expression in serotonergic and/or dopaminergic neurons of Drosophila melanogaster induces neurodegeneration, as well as motor and non-motor PD like symptoms. In this work, we examined the contribution of the serotonergic and dopaminergic circuits in the development of PD-like phenotypes. We found that olfactory and visual symptoms are majorly contributed by the serotonergic system, and that motor symptoms and reduction in survival are mainly contributed by the dopaminergic system. Chronic nicotine treatment was able to suppress several of these symptoms. These results indicate that both the serotonergic and dopaminergic systems contribute to different aspects of PD symptomatology and that nicotine has beneficial effects on specific symptoms., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2023 Carvajal-Oliveros et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Published: 2023
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43. Begomovirus Tomato Leaf Curl New Delhi Virus Is Seedborne but Not Seed Transmitted in Melon.

Author: Fortes IM, Pérez-Padilla V, Romero-Rodríguez B, Fernández-Muñoz R, Moyano C, Castillo AG, De León L, and Moriones E
Subjects: Plant Diseases, Seeds, Cucurbitaceae, Begomovirus
Abstract: Seed transmission can be of considerable relevance to the dissemination of plant viruses in nature and for their prevalence and perpetuation. Long-distance spread of isolates of the begomovirus species Tomato leaf curl New Delhi virus (genus Begomovirus , family Geminiviridae ) has recently occurred from Asia to the Middle East and the Mediterranean Basin. Here, we investigated the possible transmission by melon ( Cucumis melo L.) seeds of a tomato leaf curl New Delhi virus (ToLCNDV) isolate of the "Spain" strain widely distributed in the Mediterranean area as an alternative mechanism for long-distance spread. PCR amplification detection of ToLCNDV in floral parts and mature seeds of melon plants reveals that this virus is seedborne. "Seedborne" is defined as the ability of a virus to be carried through seeds, which does not necessarily lead to transmission to the next generation. Treatment with a chemical disinfectant significantly reduced the detectable virus associated with melon seeds, suggesting ToLCNDV contamination of the external portion of the seed coat. Also, when the internal fraction of the mature seed (seed cotyledons + embryo) was analyzed by quantitative PCR amplification, ToLCNDV was detectable at low levels, suggesting the potential for viral contamination or infection of the internal portions of seed. However, grow-out studies conducted with melon progeny plants germinated from mature seeds collected from ToLCNDV-infected plants and evaluated at early (1-leaf) or at late (20-leaf) growth stages did not support the transmission of ToLCNDV from seeds to offspring.
Published: 2023
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44. The BE (2)-M17 cell line has a better dopaminergic phenotype than the traditionally used for Parkinson´s research SH-SY5Y, which is mostly serotonergic.

Author: Carvajal-Oliveros A, Uriostegui-Arcos M, Zurita M, Melchy-Perez EI, Narváez-Padilla V, and Reynaud E
Abstract: SH-SY5Y is a cell line derived from human neuroblastoma. It is one of the most widely used in vitro models to study Parkinson's disease. Surprisingly, it has been found that it does not develop a dopaminergic phenotype after differentiation, questioning its usefulness as a Parkinson's model. There are other in vitro models with better dopaminergic characteristics. BE (2)-M17 is a human neuroblastoma cell line that differentiates when treated with retinoic acid. We compared the dopaminergic and serotonergic properties of both cell lines. BE (2)-M17 has higher basal levels of dopaminergic markers and acquires a serotonergic phenotype during differentiation while maintaining the dopaminergic phenotype. SH-SY5Y has higher basal levels of serotonergic markers but does not acquire a dopaminergic phenotype upon differentiation., Competing Interests: The authors declare no competing interests., (© 2022 The Authors.)
Published: 2022
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45. A Single Nucleotide Polymorphism Translates into a Radical Amino Acid Substitution at the Ligand-Binding Site in Fasciola hepatica Carboxylesterase B.

Author: Miranda-Miranda E, Scarcella S, Reynaud E, Narváez-Padilla V, Neira G, Mera-Y-Sierra R, Aguilar-Díaz H, and Cossio-Bayugar R
Subjects: Animals, Carboxylesterase genetics, Carboxylesterase metabolism, Amino Acid Substitution, Ligands, Polymorphism, Single Nucleotide genetics, Lysine, Glutamic Acid genetics, Xenobiotics, Binding Sites, Esters, Serine, Fasciola hepatica genetics, Fasciola hepatica metabolism, Anthelmintics pharmacology
Abstract: Fasciola hepatica anthelmintic resistance may be associated with the catalytic activity of xenobiotic metabolizing enzymes. The gene expression of one of these enzymes, identified as carboxylesterase B (CestB), was previously described as inducible in adult parasites under anthelmintic treatment and exhibited a single nucleotide polymorphism at position 643 that translates into a radical amino acid substitution at position 215 from Glutamic acid to Lysine. Alphafold 3D models of both allelic sequences exhibited a significant affinity pocket rearrangement and different ligand-docking modeling results. Further bioinformatics analysis confirmed that the radical amino acid substitution is located at the ligand affinity site of the enzyme, affecting its affinity to serine hydrolase inhibitors and preferences for ester ligands. A field genotyping survey from parasite samples obtained from two developmental stages isolated from different host species from Argentina and Mexico exhibited a 37% allele distribution for 215E and a 29% allele distribution for 215K as well as a 34% E/K heterozygous distribution. No linkage to host species or geographic origin was found in any of the allele variants.
Published: 2022
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46. α-Catenin-dependent vinculin recruitment to adherens junctions is antagonistic to focal adhesions.

Author: Bejar-Padilla V, Cabe JI, Lopez S, Narayanan V, Mezher M, Maruthamuthu V, and Conway DE
Subjects: Catenins metabolism, Cell Adhesion, Vinculin metabolism, alpha Catenin metabolism, Adherens Junctions metabolism, Focal Adhesions metabolism
Abstract: Vinculin is a protein found in both focal adhesions (FAs) and adherens junctions (AJs) which regulates actin connectivity to these structures. Many studies have demonstrated that mechanical perturbations of cells result in enhanced recruitment of vinculin to FAs and/or AJs. Likewise, many other studies have shown "cross-talk" between FAs and AJs. Vinculin itself has been suggested to be a probable regulator of this adhesion cross-talk. In this study we used MDCK as a model system of epithelia, developing cell lines in which vinculin recruitment was reduced or enhanced at AJs. Careful analysis of these cells revealed that perturbing vinculin recruitment to AJs resulted in a reduction of detectable FAs. Interestingly the cross-talk between these two structures was not due to a limited pool of vinculin, as increasing expression of vinculin did not rescue FA formation. Instead, we demonstrate that vinculin translocation between AJs and FAs is necessary for actin cytoskeleton rearrangements that occur during cell migration, which is necessary for large, well-formed FAs. Last, we show using a wound assay that collective cell migration is similarly hindered when vinculin recruitment is reduced or enhanced at AJs, highlighting that vinculin translocation between each compartment is necessary for efficient collective migration.
Published: 2022
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47. Transcriptome-Based Identification of a Functional Fasciola hepatica Carboxylesterase B.

Author: Pedroza-Gómez YJ, Cossio-Bayugar R, Aguilar-Díaz H, Scarcella S, Reynaud E, Sanchez-Carbente MDR, Narváez-Padilla V, and Miranda-Miranda E
Abstract: Bioinformatics analysis of the complete transcriptome of Fasciola hepatica, identified a total of ten putative carboxylesterase transcripts, including a 3146 bp mRNA transcript coding a 2205 bp open reading frame that translates into a protein of 735 amino acids, resulting in a predicted protein mass of 83.5 kDa and a putative carboxylesterase B enzyme. The gene coding for this enzyme was found in two reported F. hepatica complete genomes stretching 23,230 bp, containing two exons of 1282 and 1864 bp, respectively, as well as a 20,084 bp intron between the exons. The enzymatic activity was experimentally assayed on F. hepatica protein extracts by SDS-PAGE zymograms using synthetic chromogenic substrates, confirming both the theoretical molecular weight and carboxylesterase enzymatic activity. Further bioinformatics predicted that this enzyme is an integral component of the cellular membrane that should be active as a 167 kDa homodimer complex and polyacrylamide gel electrophoresis (PAGE) zymograms experiments confirmed the analysis. Additional bioinformatics analysis showed that DNA sequences that code for this particular enzyme are highly conserved in other parasitic trematodes, although they are labeled hypothetical proteins.
Published: 2021
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48. APOE Gene Associated with Cholesterol-Related Traits in the Hispanic Population.

Author: Lozano S, Padilla V, Avila ML, Gil M, Maestre G, Wang K, and Xu C
Subjects: Aged, Aged, 80 and over, Case-Control Studies, Female, Gene Frequency, Genotype, Humans, Hypercholesterolemia ethnology, Logistic Models, Male, Middle Aged, Phenotype, Apolipoproteins E genetics, Genetic Predisposition to Disease ethnology, Hispanic or Latino genetics, Hypercholesterolemia genetics, Polymorphism, Single Nucleotide
Abstract: Genetic variants in the apolipoprotein E ( APOE ) gene are associated with lipid metabolism and lipid-related traits in the non-Hispanic population. There have been limited studies regarding the association between the APOE gene and hypercholesterolemia in the Hispanic population; therefore, our aim for this study is to examine the APOE gene's associations with cholesterol level and its related phenotypes. The APOE gene consists of three different alleles, ε2, ε3, and ε4, with ε4 being associated with dementia and cardiovascular diseases. A total of 1,382 subjects were collected from the Texas Alzheimer's Research and Care Consortium (TARCC, N = 1320) and the Initial Study of Longevity and Dementia from the Rio Grande Valley (ISLD-RGV, N = 62). Questionnaires on demographics, medical history, and blood/saliva samples were collected and APOE genotypes were performed. We observed allele frequencies of the APOE ε3 (96.7%), ε4 (22.6%) and ε2 (6.8%) alleles, respectively. Multivariable logistic regression revealed a significant association between the APOE ε4 allele and hypercholesteremia ( p = 1.8 × 10 -4 ) in our studied Hispanic population. We prove for the first time, that the APOE ε4 allele increases the risk for hypercholesterol in Hispanics. Further research is needed to confirm and supports our current findings.
Published: 2021
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49. Nicotine suppresses Parkinson's disease like phenotypes induced by Synphilin-1 overexpression in Drosophila melanogaster by increasing tyrosine hydroxylase and dopamine levels.

Author: Carvajal-Oliveros A, Domínguez-Baleón C, Zárate RV, Campusano JM, Narváez-Padilla V, and Reynaud E
Subjects: Animals, Carrier Proteins genetics, Disease Models, Animal, Dopaminergic Neurons drug effects, Dopaminergic Neurons metabolism, Drosophila melanogaster, Mice, Nerve Tissue Proteins genetics, Nicotine pharmacology, Nicotinic Agonists pharmacology, Nicotinic Agonists therapeutic use, Parkinson Disease genetics, Parkinson Disease metabolism, Carrier Proteins metabolism, Dopamine metabolism, Nerve Tissue Proteins metabolism, Nicotine therapeutic use, Parkinson Disease drug therapy, Tyrosine 3-Monooxygenase metabolism
Abstract: It has been observed that there is a lower Parkinson's disease (PD) incidence in tobacco users. Nicotine is a cholinergic agonist and is the principal psychoactive compound in tobacco linked to cigarette addiction. Different studies have shown that nicotine has beneficial effects on sporadic and genetic models of PD. In this work we evaluate nicotine's protective effect in a Drosophila melanogaster model for PD where Synphilin-1 (Sph-1) is expressed in dopaminergic neurons. Nicotine has a moderate effect on dopaminergic neuron survival that becomes more evident as flies age. Nicotine is beneficial on fly survival and motility increasing tyrosine hydroxylase and dopamine levels, suggesting that cholinergic agonists may promote survival and metabolic function of the dopaminergic neurons that express Sph-1. The Sph-1 expressing fly is a good model for the study of early-onset phenotypes such as olfaction loss one of the main non-motor symptom related to PD. Our data suggest that nicotine is an interesting therapeutic molecule whose properties should be explored in future research on the phenotypic modulators of the disease and for the development of new treatments.
Published: 2021
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50. Transcriptome assembly dataset of anthelmintic response in Fasciola hepatica .

Author: Miranda-Miranda E, Cossio-Bayugar R, Aguilar-Díaz H, Narváez-Padilla V, Sachman-Ruíz B, and Reynaud E
Abstract: Fasciola hepatica is a worldwide distributed zoonotic parasitic trematode, which causes a severe liver disease clinically known as fasciolasis in a large number of wild animals, several livestock species as well as humans, prevention and control of fasciolasis is made by massive use of anthelmintic compounds on livestock and inevitably this practice has led to the emergence of anthelmintic resistant Fasciola hepatica and there is a great scientific effort to elucidate the molecular basis of anthelmintic resistance of parasitic helminths in general and of Fasciola hepatica in particular that may lead to improved anthelmintic compounds . In our project, we sequenced the transcriptomes obtained from the anthelmintic response to Triclabendazole and Albendazole on four samples from sensitive and resistant strains of Fasciola hepatica on Illumina HiSeq 4000 Platform and generated about 10.03 Gb per sample. The average genome-mapping rate is 81.29% and the average gene-mapping rate is 62.81%. 30,105 genes were identified in which 28,669 of them are known genes and 1,237 of them are novel genes from novel coding transcripts without any known features, 20,743 novel RNA transcripts were identified of which 14,293 of them are previously unknown splicing event for known genes but no alternative splicing was detected, the remaining 5,213 transcripts were found to be long noncoding RNA., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships, which have, or could be perceived to have, influenced the work reported in this article., (© 2021 The Author(s).)
Published: 2021
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103 results on '"Padilla V"'

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