Detection ofGrapevine virus Bassociated with rugose wood (corky bark) symptoms in grapevine cv. Napoleon in Murcia (Spain).

The article focuses on the detection of Grapevine virus B associated with rugose wood symptoms in grapevine. The causal agent of the rugose wood disease complex, which includes corky bark, has been attributed to members of the Vitivirus genus, such as Grapevine virus A and Grapevine virus B. Petiole extracts of two positive plants with symptoms, in phosphate buffer containing 2.5% nicotinic acid, were used as sources for the mechanical inoculation of Nicotiana benthamiana and N. clevelandii plants.