35 results on '"Nobile, G."'
Search Results
2. Management of a High Density Clementine Orchard Inoculated with Pathogenic and Non-Pathogenic Viroids
- Author
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Rizza, S., Nobile, G., Tessitori, M., Albanese, G., La Rosa, R., and Catara, A.
- Published
- 2010
3. P193 MECHANICAL COMPRESSION DEVICE (LUCAS®) IN THE CATHETERIZATION LABORATORY: RETROSPECTIVE ANALYSIS OF A SINGLE CENTER
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Dal Passo, B, primary, Cardelli, L, additional, Capecchi, A, additional, Nobile, G, additional, Canovi, L, additional, Frascaro, F, additional, Zanarelli, L, additional, Piscitelli, L, additional, and Casella, G, additional
- Published
- 2022
- Full Text
- View/download PDF
4. Multi-Criteria Experimental Comparison of Batteries Circuital Models for Automotive Applications
- Author
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Nobile, G., Cacciato, M., Scarcella, G., and Giacomo Scelba
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Economics and Econometrics ,Energy storage systems ,SOC estimation ,Computer Networks and Communications ,Mechanical Engineering ,Automotive Engineering ,Automotive ,Battery modeling ,SOH estimation ,Transportation ,Electrical and Electronic Engineering - Published
- 2018
5. Real time RT-PCR assay for quantitative detection of Citrus viroid III in plant tissues
- Author
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Rizza, S., Nobile, G., Tessitori, M., Catara, A., and Conte, E.
- Published
- 2009
6. Losses Minimization Control for an Integrated Multi- Drives Topology devoted to Hybrid Electric Vehicles [IEEE Transactions on Industrial Electronics, 2019]
- Author
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Nobile, G., Scelba, G., Cacciato, M., and Scarcella, G.
- Subjects
multi-winding motor drives ,Automotive ,maximum torque per ampere ,energy efficiency ,hybrid energy storage ,hybrid electric vehicles ,induction motor drives ,starter-alternator - Published
- 2019
7. Analysis of the structural parameters of an a-Si:H n+-i-n+ structure by numerical simulations
- Author
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Eray, A. and Nobile, G.
- Published
- 2003
8. Analysis of the structural parameters of an a-Si : H n+-i-n+ structure by numerical simulations
- Author
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Eray, A. and Nobile, G.
- Published
- 2003
- Full Text
- View/download PDF
9. AMPS-1D Modeling of a-Si:H n+-i-n+ Structure: the Validity of Space Charge Limited Current Analysis
- Author
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ERAY, A. and NOBILE, G.
- Subjects
AMPS-1D,Hydrogenated amorphous silicon,n+-i-n+ structure,SCLC,activation energy - Abstract
In this paper, the AMPS-1D (Analysis of Microelectronic and photonic structure) simulation program is used to understand the origin of the differences observed in Space Charge Limited Current (SCLC) analysis in thin and thick a-Si:H n + -i- n+ structure. For that purpose, the problem of applicability of SCLC measurements to n+-i-n+ a-Si:H samples are investigated by using both the thin (0.3 m m) and thick (3 m m) samples. The simulation results show that activation energy in thick samples is larger than in thinner samples, which are an agreement with the experimental results. It is emphasized that this method is highly useful for good quality a-Si:H samples, even if it is a thin sample, and den Boer analysis gives correct information about the density of states. This information comes from the states in the upper limited part of the gap, as exhibited by the lower activation energy of thin samples.
- Published
- 2014
10. The immunological and virological consequences of planned treatment interruptions in children with HIV infection
- Author
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Klein, Nigel, Sefe, Delali, Mosconi, Ilaria, Zanchetta, Marisa, Castro, Hannah, Jacobsen, Marianne, Jones, Hannah, Bernardi, Stefania, Pillay, Deenan, Giaquinto, Carlo, Walker, A. Sarah, Gibb, Diana M., De Rossi, Anita, Paediatric, European Network for Treatment of AIDS 11 Trial Team including Aboulker JP, Ananworanich, J, Babiker, A, Belfrage, E, Bernardi, S, Blanche, S, Bohlin, Ab, Bologna, R, Burger, Dm, Butler, K, Castelli Gattinara, G, Castro, H, Clayden, P, Compagnucci, A, Darbyshire, Jh, Debré, M, Faye, A, de Groot, R, della Negra, M, Duiculescu, D, Giaquinto, C, Gibb, Dm, Grosch Wörner, I, Hainault, M, Harper, L, Klein, N, Lallemant, M, Levy, J, Lyall, H, Marczynska, M, Mardarescu, M, Mellado Peña, Mj, Nadal, D, Niehues, T, Peckham, C, Pillay, D, Ramos Amador, Jt, Rosado, L, Rosso, R, Rudin, C, Saidi, Y, Scherpbier, Hj, Sharland, M, Stevanovic, M, Thorne, C, Tovo, Pier Angelo, Tudor Williams, G, Valerius, N, Walker, As, Welch, S, Wintergerst, U, Aboulker, Jp, Mofenson, L, Moye, J, Saïdi, Y, Cressey, Tr, Jacqz Aigrain, E, Khoo, S, Tréluyer, Jm, De Rossi, A, Ngo Giang Huong, N, Muñoz Fernandez, Ma, Hill, C, Lepage, P, Pozniak, A, Vella, S, Hadjou, G, Léonardo, S, Riault, Y, Buck, L, Farrelly, L, Forcat, S, Harrison, L, Horton, J, Johnson, D, Moore, S, Taylor, C, Chalermpantmetagul, S, Peongjakta, R, Chailert, S, Fregonese, F, Jourdain, G, Butler, D, Carlton, C, Collins, D, Kao, G, Van Buskirk, S, Watson, S, Corradini, S, Floret, D, Le Thi, Tt, Monpoux, F, Cottalorda, J, Lefebvre, Jc, Mellul, S, Boudjoudi, N, Firtion, G, Denon, M, Picard, F, Beniken, D, Damond, F, Alexandre, G, Tricoire, J, Nicot, F, Krivine, A, Rivaux, D, Chaix, Ml, Notheis, G, Strotmann, G, Schlieben, S, Rampon, O, Zanchetta, M, Ginocchio, F, Viscoli, C, Martino, A, Pontrelli, G, Concato, C, Mazza, A, Rossetti, G, Dobosz, S, Oldakowska, A, Popielska, J, Kaflik, M, Stanczak, J, Stanczack, G, Dyda, T, González Tomé, Mi, Delgado García, R, Fernandez Gonzalez, Mt, Martín Fontelos, P, Piñeiro Pérez, R, Penin, M, Garcia Mellado, I, Medina, Af, Ascencion, B, Garcia Bermejo, I, Garcia Vela, Ja, Martin Rubio, I, Gurbindo, D, Navarro Gomez, Ml, Jimenez, Jl, Garcia Torre, A, José Gómez, Mi, García Rodriguez, Mc, Moreno Pérez, D, Núñez Cuadros, E, Asensi Botet, F, Pérez, A, Pérez Tamarit, Md, Gobernado Serrano, M, Gonzales Molina, A, Kalhert, C, Dobrovoljac, M, Berger, C, Nobile, G, Reinhard, S, Schupbach, J, Bunupuradah, T, Puthanakit, T, Pancharoen, C, Butterworth, O, Phasomsap, C, Jupimai, T, Ubolyam, S, Phanuphak, P, Mai, C, Kanjanavanit, S, Namwong, T, Chutima, D, Raksasang, M, Foster, C, Hamadache, D, Campbell, S, Newbould, C, Monrose, C, Patel, D, Kaye, S, Seery, P, Wildfire, A, Novelli, V, Shingadia, D, Moshal, K, Flynn, J, Clapson, M, Allen, A, Spencer, L, Depala, M, Jacobsen, M, Mcmaster, P, Phipps, M, Orendi, J, Farmer, C, Liebeschuetz, S, Sodeinde, O, Wong, S, Heath, Y, Scott, S, Gandhi, K, Lewis, P, Daglish, J, Weiner, L, Famiglietti, M, Rana, S, Yu, P, Roa, J, Puga, A, Haerry, A, and Inma, A.
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CD31 ,Genetics and Molecular Biology (all) ,CD4-Positive T-Lymphocytes ,Time Factors ,T-CELL RECONSTITUTION ,ACTIVE ANTIRETROVIRAL THERAPY, STRUCTURED TREATMENT INTERRUPTION, T-CELL RECONSTITUTION, HIV-1-INFECTED CHILDREN, IMMUNE RECONSTITUTION, THYMIC OUTPUT, 1-INFECTED CHILDREN ,Adolescent ,Anti-Retroviral Agents ,CD8-Positive T-Lymphocytes ,Child ,Child, Preschool ,Drug Administration Schedule ,HIV Infections ,Humans ,Immunophenotyping ,Lymphocyte Count ,Treatment Outcome ,Viral Load ,Agricultural and Biological Sciences (all) ,Biochemistry, Genetics and Molecular Biology (all) ,Medicine (all) ,Biochemistry ,law.invention ,IMMUNE RECONSTITUTION ,0302 clinical medicine ,Randomized controlled trial ,law ,030212 general & internal medicine ,HIV-1-INFECTED CHILDREN ,0303 health sciences ,Multidisciplinary ,ACTIVE ANTIRETROVIRAL THERAPY ,3. Good health ,Medicine ,Off Treatment ,Poverty-related infectious diseases Infectious diseases and international health [N4i 3] ,THYMIC OUTPUT ,Viral load ,Research Article ,Science ,1-INFECTED CHILDREN ,Auto-immunity, transplantation and immunotherapy [N4i 4] ,03 medical and health sciences ,Acquired immunodeficiency syndrome (AIDS) ,medicine ,Preschool ,030304 developmental biology ,business.industry ,medicine.disease ,Clinical trial ,Immunology ,STRUCTURED TREATMENT INTERRUPTION ,business ,CD8 - Abstract
Contains fulltext : 126098.pdf (Publisher’s version ) (Open Access) OBJECTIVES: To evaluate the immunological and viral consequences of planned treatment interruptions (PTI) in children with HIV. DESIGN: This was an immunological and virological sub-study of the Paediatric European Network for Treatment of AIDS (PENTA) 11 trial, which compared CD4-guided PTI of antiretroviral therapy (ART) with continuous therapy (CT) in children. METHODS: HIV-1 RNA and lymphocyte subsets, including CD4 and CD8 cells, were quantified on fresh samples collected during the study; CD45RA, CD45RO and CD31 subpopulations were evaluated in some centres. For 36 (18 PTI, 18 CT) children, immunophenotyping was performed and cell-associated HIV-1 DNA analysed on stored samples to 48 weeks. RESULTS: In the PTI group, CD4 cell count fell rapidly in the first 12 weeks off ART, with decreases in both naive and memory cells. However, the proportion of CD4 cells expressing CD45RA and CD45RO remained constant in both groups. The increase in CD8 cells in the first 12 weeks off ART in the PTI group was predominantly due to increases in RO-expressing cells. PTI was associated with a rapid and sustained increase in CD4 cells expressing Ki67 and HLA-DR, and increased levels of HIV-1 DNA. CONCLUSIONS: PTI in children is associated with rapid changes in CD4 and CD8 cells, likely due to increased cell turnover and immune activation. However, children off treatment may be able to maintain stable levels of naive CD4 cells, at least in proportion to the memory cell pool, which may in part explain the observed excellent CD4 cell recovery with re-introduction of ART.
- Published
- 2013
11. Effect of severe and mild Citrus Tristeza Virus isolates on selected genes expression of different citrus hosts
- Author
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Licciardello, G., Tessitori, Matilde, LA ROSA, Rosa, Nobile, G., Catara, A., and Rizza, S.
- Published
- 2011
12. High density citrus orchard sustainability through a non-pathogenetic viroid
- Author
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Rizza, S, Nobile, G, Tessitori, Matilde, LA ROSA, Rosa, and Catara, A.
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- 2007
13. Education Enhancement on three-phase system measurements
- Author
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Graziani, Salvatore, Nobile, G, Pitrone, N, and Savalli, N.
- Published
- 2007
14. TWELVE YEARS MANAGMENT OF A HIGH DENSITY CLEMENTINE ORCHARD INOCULATED WITH PATHOGENIC AND NON-PATHOGENIC VIROIDS
- Author
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Rizza, S, Nobile, G, Tessitori, Matilde, Albanese, G, LA ROSA, Rosa, and Catara, A.
- Published
- 2007
15. Twelve years management of high density Clementine orchards inoculated with pathogenic and non-pathogenic viroids
- Author
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Rizza, S, Nobile, G, Tessitori, Matilde, Albanese, G, LA ROSA, Rosa, and Catara, A.
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- 2007
16. Field effect a-Si:H solar cells
- Author
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Caputo, Domenico, DE CESARE, Giampiero, Palma, Fabrizio, Nobile, G., and Tucci, M.
- Published
- 2002
17. Study on structural, electrical and optical properties of microc-Si:H and microc-SiC:H films
- Author
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Demichelis, F., Crovini, G., Pirri, Candido, Tresso, Elena Maria, Conte, G., Nobile, G., Rubino, A., and Terzini, E.
- Published
- 1993
18. New platinum(II9 complexes of beta-cyclodextrin diamine derivatives and their antitumor activity
- Author
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Cucinotta, V, Mangano, A, Nobile, G, Santoro, A. M., and Vecchio, Graziella
- Published
- 1993
19. Real time RT-PCR assay for quantitative detection ofCitrus viroid IIIin plant tissues
- Author
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Rizza, S., primary, Nobile, G., additional, Tessitori, M., additional, Catara, A., additional, and Conte, E., additional
- Published
- 2009
- Full Text
- View/download PDF
20. AMPS-1D Modeling of a-Si:H n + -i-n + Structure: the Validity of Space Charge Limited Current Analysis.
- Author
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Eray, A. and Nobile, G.
- Subjects
- *
MICROELECTRONICS , *PHOTONICS , *SPACE charge , *ELECTRIC currents , *PHYSICS - Abstract
In this paper, the AMPS-1D (Analysis of Microelectronic and photonic structure) simulation program is used to understand the origin of the differences observed in Space Charge Limited Current (SCLC) analysis in thin and thick a-Si:H n + -i- n + structure. For that purpose, the problem of applicability of SCLC measurements to n+ -i-n + a-Si:H samples are investigated by using both the thin (0.3 µm) and thick (3 µm) samples. The simulation results show that activation energy in thick samples is larger than in thinner samples, which are an agreement with the experimental results. It is emphasized that this method is highly useful for good quality a-Si:H samples, even if it is a thin sample, and den Boer analysis gives correct information about the density of states. This information comes from the states in the upper limited part of the gap, as exhibited by the lower activation energy of thin samples. [ABSTRACT FROM AUTHOR]
- Published
- 2004
21. Analysis of the structural parameters of an a-Si : H n+-i-n+structure by numerical simulations
- Author
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Eray, A. and Nobile, G.
- Abstract
In this study, we discuss measurements of the J–V characteristics of an a-Si : H n+-i-n+structure, and the results of numerical simulation using the AMPS-1D simulation program. Application of the AMPS simulation model to the sample structures considered allows us to determine the structural properties of the a-Si : H n+-i-n+structure. The one-dimensional simulation program was examined in n+-i-n+structures with different qualities and thicknesses of the i-layer. We find that the n+/i interface is more abrupt for a device with a high density of states (DOS), resulting in high values of the activation energy (Eact). In contrast, thin samples of good quality (low DOS) give low values of Eact. Analysis by numerical modeling confirms the experimental results.
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- 2003
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22. Effects of the ink concentration on multi-layer gravure-printed PEDOT:PSS
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Anna De Girolamo Del Mauro, Carla Minarini, Giuseppe Nobile, Antonio Imparato, Maria Montanino, Giuliano Sico, Minarini, C., Nobile, G., Imparato, A., De Girolamo Del Mauro, A., Montanino, M., and Sico, G.
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Materials science ,Nanotechnology ,02 engineering and technology ,engineering.material ,010402 general chemistry ,01 natural sciences ,Biomaterials ,PEDOT:PSS ,Coating ,Ink concentration profile ,Materials Chemistry ,Electrical and Electronic Engineering ,Sheet resistance ,Organic electronics ,Inkwell ,Multi-layer ,ITO free ,General Chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,0104 chemical sciences ,Electronic, Optical and Magnetic Materials ,Gravure printing ,Highly conductive PEDOT:PSS ,PEN ,Printed electronics ,Electrode ,engineering ,0210 nano-technology ,Layer (electronics) - Abstract
To date, highly conductive PEDOT:PSS is the most promising transparent electrode for printing-based flexible organic electronics. Spin-coating and slot-die coating have been commonly used for printing this material. Among the roll-to-roll printing processes, gravure is the most promising for manufacturing large area electronics offering the advantages of high speed and high printing definition. However, gravure printing highly conductive PEDOT encounters some technological limitations such as low thickness, layer inhomogeneity and high surface roughness resulting in a layer not suitable as electrode in electronic devices. In order to realize an electrode of highly conductive PEDOT by gravure printing, a multilayer approach with variable ink concentration was tried using IPA as process solvent. Variable solvent amount of overlapped printed layers was found to play an important role in the spreading of the PEDOT ink onto the pre-printed layers and in the smoothing of its existent peaks. In particular, adopting increasing ink dilution with increasing of the overlapped layers, multi-layer gravure-printed highly conductive PEDOT was successfully realized with characteristics suitable as transparent electrode for organic electronic devices (sheet resistance lower than 130 Ω/sq, conductivity higher than 450 S/cm and optical transmittance over 80%). This is the first time that such results were reached by gravure printing technique thanks to the easy proposed approach. © 2015 Elsevier B.V.
- Published
- 2016
23. Gravure-printed PEDOT:PSS on flexible PEN substrate as ITO-free anode for polymer solar cells
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Maria Montanino, R. Diana, Giuliano Sico, Carla Minarini, A. De Girolamo Del Mauro, Antonio Imparato, Rosa Ricciardi, Giuseppe Nobile, Pasquale Morvillo, M. Tesoro, Minarini, C., Sico, G., Imparato, A., Nobile, G., Morvillo, P., Diana, R., Ricciardi, R., Tesoro, M., De Girolamo Del Mauro, A., and Montanino, M.
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Conductive polymer ,Spin coating ,Materials science ,Polymers and Plastics ,General Chemistry ,Substrate (printing) ,Butyric acid, Conducting polymers, Conductive films, Electrodes, Graphic methods, Heterojunctions, Offset printing, Plastic coatings, Polymers, Printing, Spin coating, Styrene Substrates, Tin oxides ,Polymer solar cell ,Anode ,Indium tin oxide ,PEDOT:PSS ,Materials Chemistry ,Ceramics and Composites ,Composite material ,Polyethylene naphthalate - Abstract
In this work, highly conductive and transparent films based on poly(3,4 ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) doped with dimethyl sulfoxide (DMSO) were printed onto a flexible substrate, the polyethylene naphthalate (PEN), using the gravure printing method. Gravure-printed DMSO-PEDOT:PSS ink suitably modified with isopropanol was developed and used as anode in flexible solar cells replacing the commonly used anode, that is, Indium Tin Oxide (ITO). Several inks were prepared and characterized in terms of viscosity and surface energy and tested by gravure printing. The wettability of the PEN substrate was investigated through contact angle measurements. For comparison, the anodic conductive polymer was also spin coated on the PEN substrate using an ink suitable for this technique. The films produced with both the techniques, gravure printing and spin coating, each using a proper ink, were characterized in terms of structure and physical–chemical properties. The printed layers were employed as anodes into ITO free-polymer solar cells based on a bulk heterojunction of Poly({4,8-bis[(2-ethylhexyl)oxy]benzo[1,2-b:4,5-b′]dithiophene-2,6-diyl}{3-fluoro-2-[(2-ethylhexyl)carbonyl]thieno [3,4-b]thiophenediyl}) (PTB7) and [6,6]-phenyl C70-butyric acid methyl ester ([70]PCBM). The performances of the devices with printed and spin coated polymeric anode were compared. The cell with the spin-coated anode reached a power conversion efficiency of 3% while the corresponding device with the printed anode a value around 2%. POLYM. COMPOS., 36:1104–1109, 2015. © 2015 Society of Plastics Engineers
- Published
- 2015
24. Trends, outcomes, and complications of surgery for lesional epilepsy in infants and toddlers: A multicenter study.
- Author
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Barba C, Pelliccia V, Grisotto L, De Palma L, Nobile G, Gozzo F, Revay M, Carfi-Pavia G, Cossu M, Giordano F, Consales A, De Benedictis A, Cavallini E, Mion C, Accolla C, Specchio N, Nobili L, Guerrini R, and Tassi L
- Subjects
- Humans, Male, Female, Infant, Retrospective Studies, Child, Preschool, Treatment Outcome, Epilepsy surgery, Postoperative Complications epidemiology, Neurosurgical Procedures adverse effects
- Abstract
Objective: To assess seizure and developmental outcomes, their predictors, and complications in 160 children who, between 1998 and 2022, underwent surgery for lesional epilepsy with curative intent before the age of 3 years. To compare trends in epilepsy surgery in this age group before and after the year 2014., Methods: Retrospective multicenter study. Descriptive and univariate analyses, and multivariable models for all outcomes., Results: These 160 patients (76 F; 47.5%) underwent 169 surgeries (age at surgery 20.4 ± 9.4 months). At the last follow-up (77 ± 57.4 months), 121 patients (75.6%) were in Engel class I, 106 (66.2%) of whom were in Engel class Ia. Antiseizure medications were stopped in 84 patients (52.5%). Complications requiring reoperations were observed in 16 patients (10%; 9.5% of surgeries) and unexpected permanent deficits in 12 (7.5%; 7.1% of surgeries). Postoperative cognitive functions remained unchanged in 56 patients (44.4%), improved in 51 (40.5%), and worsened in 19 (15.1%). Multivariable analyses showed that the probability of achieving Engel class Ia was lower when the duration of epilepsy was longer, patients underwent preoperative video-EEG, and unexpected postoperative permanent deficits occurred. Cognitive improvement after surgery was associated with lower preoperative seizure frequency, better preoperative developmental level, and a longer postoperative follow-up. FCDII and tumors were the histopathologies carrying a higher probability of achieving seizure freedom, while polymicrogyria was associated with a lower probability of cognitive improvement. The number of patients operated on after 2014 was higher than before (61.3% vs. 38.7%), with stable outcomes., Significance: Epilepsy surgery is effective and safe in infants and toddlers, although the complication rate is higher than seen in older patients. Shorter duration of epilepsy, lower seizure frequency, no need for video-EEG, tumors, and some malformations of cortical development are robust predictors of seizure and cognitive outcome that may be exploited to increase earlier referral., Plain Language Summary: This study analyzed the results of epilepsy surgery in 160 children who had been operated on before the age of 3 years at four Italian centers between 1998 and 2022. At the last follow-up (77 ± 57.4 months), 121 patients (75.6%) were free from disabling seizures, of which 106 (66.2%) were completely seizure-free since surgery. Major surgical complications occurred in 28 patients (17.5%), which is higher than observed with epilepsy surgery in general, but similar to hemispheric/multilobar surgery. Postoperative cognitive function remained unchanged in 56 patients (44.4%), improved in 51 (40.5%), and worsened in 19 (15.1%). Epilepsy surgery is effective and safe in infants and toddlers., (© 2024 The Author(s). Epilepsia Open published by Wiley Periodicals LLC on behalf of International League Against Epilepsy.)
- Published
- 2024
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- View/download PDF
25. Role of the I416L Variant of Complement Factor I in Thrombotic Microangiopathy Among Patients of African Ancestry.
- Author
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Nobile G, Doreille A, Raymond L, Robert T, Dancer M, Mesnard L, and Rafat C
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- 2023
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26. Automatic video analysis and classification of sleep-related hypermotor seizures and disorders of arousal.
- Author
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Moro M, Pastore VP, Marchesi G, Proserpio P, Tassi L, Castelnovo A, Manconi M, Nobile G, Cordani R, Gibbs SA, Odone F, Casadio M, and Nobili L
- Subjects
- Humans, Seizures diagnosis, Seizures complications, Sleep, Arousal, Video Recording methods, Electroencephalography methods, Epilepsy, Reflex
- Abstract
Objective: Sleep-related hypermotor epilepsy (SHE) is a focal epilepsy with seizures occurring mostly during sleep. SHE seizures present different motor characteristics ranging from dystonic posturing to hyperkinetic motor patterns, sometimes associated with affective symptoms and complex behaviors. Disorders of arousal (DOA) are sleep disorders with paroxysmal episodes that may present analogies with SHE seizures. Accurate interpretation of the different SHE patterns and their differentiation from DOA manifestations can be difficult and expensive, and can require highly skilled personnel not always available. Furthermore, it is operator dependent., Methods: Common techniques for human motion analysis, such as wearable sensors (e.g., accelerometers) and motion capture systems, have been considered to overcome these problems. Unfortunately, these systems are cumbersome and they require trained personnel for marker and sensor positioning, limiting their use in the epilepsy domain. To overcome these problems, recently significant effort has been spent in studying automatic methods based on video analysis for the characterization of human motion. Systems based on computer vision and deep learning have been exploited in many fields, but epilepsy has received limited attention., Results: In this paper, we present a pipeline composed of a set of three-dimensional convolutional neural networks that, starting from video recordings, reached an overall accuracy of 80% in the classification of different SHE semiology patterns and DOA., Significance: The preliminary results obtained in this study highlight that our deep learning pipeline could be used by physicians as a tool to support them in the differential diagnosis of the different patterns of SHE and DOA, and encourage further investigation., (© 2023 The Authors. Epilepsia published by Wiley Periodicals LLC on behalf of International League Against Epilepsy.)
- Published
- 2023
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27. Biallelic ZBTB11 variants associated with complex neuropsychiatric phenotype featuring Tourette syndrome.
- Author
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Scala M, De Grandis E, Nobile G, Iacomino M, Madia F, Capra V, Nobili L, Zara F, and Striano P
- Subjects
- Humans, Phenotype, Tourette Syndrome genetics, Tourette Syndrome psychology, Obsessive-Compulsive Disorder
- Published
- 2023
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28. Measuring Active Power as the Difference between the Peak Value of Instantaneous Power and the Apparent Power.
- Author
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Nobile G, Cacciato M, and Vasta E
- Abstract
The traditional approach to calculate the active and reactive power in AC power systems requires the measurement of the phase shift between the voltage and current for the evaluation of the power factor. To do this, power analyzers can implement several methods. In principle, it is always necessary to identify specific points of waveforms (e.g., using a zero-crossing detection technique) and get their time shift. In a similar way, the frequency value must be evaluated in order to calculate the angular frequency. Unfortunately, this kind of common method exhibits some issues, such as the large sensitivity to noise. Moreover, inaccuracies in the evaluation of the power factor have a big impact on the final estimation of the electric power. This paper presents a simple but effective way to calculate the electric power, overcoming the need for a direct measurement of the phase shift and frequency. In particular, it is shown that the active power can be easily calculated as the difference between the peak value of the instantaneous power and apparent power. The reactive power and power factor are evaluated by exploiting the same quantities. The practical implementation of the proposed formulation in power analyzers guarantees several benefits without reducing accuracy.
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- 2022
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29. A Phenotypic-Driven Approach for the Diagnosis of WOREE Syndrome.
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Riva A, Nobile G, Giacomini T, Ognibene M, Scala M, Balagura G, Madia F, Accogli A, Romano F, Tortora D, Severino M, Scudieri P, Baldassari S, Musante I, Uva P, Salpietro V, Torella A, Nigro V, Capra V, Nobili L, Striano P, Mancardi MM, Zara F, and Iacomino M
- Abstract
Background: WOREE syndrome is a rare neurodevelopmental disorder featuring drug-resistant epilepsy and global developmental delay. The disease, caused by biallelic pathogenic variants in the WWOX gene, usually leads to severe disability or death within the first years of life. Clinicians have become more confident with the phenotypic picture of WOREE syndrome, allowing earlier clinical diagnosis. We report a boy with a peculiar clinic-radiological pattern supporting the diagnosis of WOREE syndrome., Methods: DNA was extracted from blood samples of the proband and his parents and subjected to Exome Sequencing (ES). Agarose gel electrophoresis, real-time quantitative PCR (Q-PCR), and array-CGH 180K were also performed., Results: ES detected a pathogenic stop variant (c.790C > T, p.Arg264*) in one allele of WWOX in the proband and his unaffected mother. A 180K array-CGH analysis revealed a 84,828-bp (g.chr16:78,360,803-78,445,630) deletion encompassing exon 6. The Q-PCR product showed that the proband and his father harbored the same deleted fragment, fusing exons 5 and 7 of WWOX ., Conclusions: Genetic testing remains crucial in establishing the definitive diagnosis of WOREE syndrome and allows prenatal interventions/parental counseling. However, our findings suggest that targeted Next Generation Sequencing-based testing may occasionally show technical pitfalls, prompting further genetic investigation in selected cases with high clinical suspicion., Competing Interests: AR has received honoraria from Kolfarma srl and Proveca Pharma Ltd. PSt has served on a scientific advisory board for the Italian Agency of the Drug (AIFA); has received honoraria from GW pharma, Kolfarma srl, Proveca Pharma Ltd., and Eisai Inc.; and has received research support from the Italian Ministry of Health and Fondazione San Paolo. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Riva, Nobile, Giacomini, Ognibene, Scala, Balagura, Madia, Accogli, Romano, Tortora, Severino, Scudieri, Baldassari, Musante, Uva, Salpietro, Torella, Nigro, Capra, Nobili, Striano, Mancardi, Zara and Iacomino.)
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- 2022
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30. Comparison of Qualitative and Quantitative Analyses of MR-Arterial Spin Labeling Perfusion Data for the Assessment of Pediatric Patients with Focal Epilepsies.
- Author
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Tortora D, Cataldi M, Severino M, Consales A, Pacetti M, Parodi C, Sertorio F, Ramaglia A, Cognolato E, Nobile G, Mancardi M, Prato G, Siri L, Giacomini T, Striano P, Arnaldi D, Piatelli G, Rossi A, and Nobili L
- Abstract
The role of MR Arterial-Spin-Labeling Cerebral Blood Flow maps (ASL-CBF) in the assessment of pediatric focal epilepsy is still debated. We aim to compare the Seizure Onset Zone (SOZ) detection rate of three methods of evaluation of ASL-CBF: 1) qualitative visual (qCBF), 2) z-score voxel-based quantitative analysis of index of asymmetry (AI-CBF), and 3) z-score voxel-based cluster analysis of the quantitative difference of patient’s CBF from the normative data of an age-matched healthy population (cCBF). Interictal ASL-CBF were acquired in 65 pediatric patients with focal epilepsy: 26 with focal brain lesions and 39 with a normal MRI. All hypoperfusion areas visible in at least 3 contiguous images of qCBF analysis were identified. In the quantitative evaluations, clusters with a significant z-score AI-CBF ≤ −1.64 and areas with a z-score cCBF ≤ −1.64 were considered potentially related to the SOZ. These areas were compared with the SOZ defined by the anatomo-electro-clinical data. In patients with a positive MRI, SOZ was correctly identified in 27% of patients using qCBF, 73% using AI-CBF, and 77% using cCBF. In negative MRI patients, SOZ was identified in 18% of patients using qCBF, in 46% using AI-CBF, and in 64% using cCBF (p < 0.001). Quantitative analyses of ASL-CBF maps increase the detection rate of SOZ compared to the qualitative method, principally in negative MRI patients.
- Published
- 2022
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- View/download PDF
31. Fishery management in a marine protected area with compliance gaps: Socio-economic and biological insights as a first step on the path of sustainability.
- Author
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Coppa S, Pronti A, Massaro G, Brundu R, Camedda A, Palazzo L, Nobile G, Pagliarino E, and de Lucia GA
- Subjects
- Europe, Italy, Socioeconomic Factors, Conservation of Natural Resources, Fisheries
- Abstract
Overfishing is one of the main impacts on the marine environment and multiple-use Marine Protected Areas (MPAs) could be a useful tool to conserve biodiversity and promote sustainable resource exploitation. However, ensuring a high level of protection on the ground is a difficult task. This work contributes to the analysis of the causes at the root of MPAs' ineffectiveness by examining the management of Paracentrotus lividus fishery in an Italian MPA, employing a multidisciplinary approach built on biological and socio-economic competences. This sea urchin species has a determinant ecological role in structuring infralittoral benthic assemblages and is the most exploited echinoid in Europe. From 2010 to 2018, underwater sampling was conducted over 39 monitoring sites to define P. lividus spatial and temporal trends. Declared catches and semi-structured interviews with local stakeholders were used to define the socio-economical context, underline existing conflicts among them, as well as to trace the historical evolution of sea urchin fishery. The results show that the management of sea urchin fishery is not sustainable, primarily because of the stakeholders' non-compliance with the rules. P. lividus stock is progressively declining (-73% in 9 years), showing no difference between MPA (0.5 ± 0.15 ind./m
2 ) and control sites (0.3 ± 0.04 ind./m2 ). Moreover, fishermen dominate the social arena while scientists, civil society and local press have little relevance. Additionally, the untruthfulness of catch declarations was proved, the IUU fishery is relevant and the black market is hiding the actual economic value. This work offers management solutions that may be useful in other areas that show similar compliance issues., (Copyright © 2020 Elsevier Ltd. All rights reserved.)- Published
- 2021
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- View/download PDF
32. Fibronectin-binding protein B (FnBPB) from Staphylococcus aureus protects against the antimicrobial activity of histones.
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Pietrocola G, Nobile G, Alfeo MJ, Foster TJ, Geoghegan JA, De Filippis V, and Speziale P
- Subjects
- Anti-Infective Agents metabolism, Cell Wall drug effects, Cell Wall metabolism, Histones metabolism, Osmolar Concentration, Plasminogen metabolism, Protein Binding, Staphylococcus aureus cytology, Adhesins, Bacterial metabolism, Anti-Infective Agents pharmacology, Histones pharmacology, Staphylococcus aureus drug effects, Staphylococcus aureus metabolism
- Abstract
Staphylococcus aureus is a Gram-positive bacterium that can cause both superficial and deep-seated infections. Histones released by neutrophils kill bacteria by binding to the bacterial cell surface and causing membrane damage. We postulated that cell wall-anchored proteins protect S. aureus from the bactericidal effects of histones by binding to and sequestering histones away from the cell envelope. Here, we focused on S. aureus strain LAC and by using an array of biochemical assays, including surface plasmon resonance and ELISA, discovered that fibronectin-binding protein B (FnBPB) is the main histone receptor. FnBPB bound all types of histones, but histone H3 displayed the highest affinity and bactericidal activity and was therefore investigated further. H3 bound specifically to the A domain of recombinant FnBPB with a K
D of 86 nm, ∼20-fold lower than that for fibrinogen. Binding apparently occurred by the same mechanism by which FnBPB binds to fibrinogen, because FnBPB variants defective in fibrinogen binding also did not bind H3. An FnBPB-deletion mutant of S. aureus LAC bound less H3 and was more susceptible to its bactericidal activity and to neutrophil extracellular traps, whereas an FnBPB-overexpressing mutant bound more H3 and was more resistant than the WT. FnBPB bound simultaneously to H3 and plasminogen, which after activation by tissue plasminogen activator cleaved the bound histone. We conclude that FnBPB provides a dual immune-evasion function that captures histones and prevents them from reaching the bacterial membrane and simultaneously binds plasminogen, thereby promoting its conversion to plasmin to destroy the bound histone., (© 2019 Pietrocola et al.)- Published
- 2019
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33. Staphylococcus aureus Manipulates Innate Immunity through Own and Host-Expressed Proteases.
- Author
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Pietrocola G, Nobile G, Rindi S, and Speziale P
- Subjects
- Bacterial Proteins metabolism, Coagulase metabolism, Complement System Proteins, Epithelial Cells immunology, Epithelial Cells microbiology, Exfoliatins, Humans, Immune Evasion immunology, Metalloendopeptidases metabolism, Neutrophils immunology, RNA-Binding Proteins metabolism, Serine Endopeptidases metabolism, Serine Proteases metabolism, Staphylococcal Infections microbiology, Staphylococcus aureus pathogenicity, Virulence Factors, von Willebrand Factor metabolism, Host-Pathogen Interactions immunology, Immunity, Innate immunology, Peptide Hydrolases metabolism, Staphylococcal Infections immunology, Staphylococcus aureus immunology, Staphylococcus aureus metabolism
- Abstract
Neutrophils, complement system and skin collectively represent the main elements of the innate immune system, the first line of defense of the host against many common microorganisms. Bacterial pathogens have evolved strategies to counteract all these defense activities. Specifically, Staphylococcus aureus , a major human pathogen, secretes a variety of immune evasion molecules including proteases, which cleave components of the innate immune system or disrupt the integrity of extracellular matrix and intercellular connections of tissues. Additionally, S. aureus secretes proteins that can activate host zymogens which, in turn, target specific defense components. Secreted proteins can also inhibit the anti-bacterial function of neutrophils or complement system proteases, potentiating S. aureus chances of survival. Here, we review the current understanding of these proteases and modulators of host proteases in the functioning of innate immunity and describe the importance of these mechanisms in the pathology of staphylococcal diseases.
- Published
- 2017
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34. Molecular Interactions of Human Plasminogen with Fibronectin-binding Protein B (FnBPB), a Fibrinogen/Fibronectin-binding Protein from Staphylococcus aureus.
- Author
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Pietrocola G, Nobile G, Gianotti V, Zapotoczna M, Foster TJ, Geoghegan JA, and Speziale P
- Subjects
- Adhesins, Bacterial metabolism, Bacterial Proteins metabolism, Humans, Plasminogen metabolism, Protein Binding, Protein Domains, Staphylococcus aureus metabolism, Adhesins, Bacterial chemistry, Bacterial Proteins chemistry, Plasminogen chemistry, Staphylococcus aureus chemistry
- Abstract
Staphylococcus aureus is a commensal bacterium that has the ability to cause superficial and deep-seated infections. Like several other invasive pathogens, S. aureus can capture plasminogen from the human host where it can be converted to plasmin by host plasminogen activators or by endogenously expressed staphylokinase. This study demonstrates that sortase-anchored cell wall-associated proteins are responsible for capturing the bulk of bound plasminogen. Two cell wall-associated proteins, the fibrinogen- and fibronectin-binding proteins A and B, were found to bind plasminogen, and one of them, FnBPB, was studied in detail. Plasminogen captured on the surface of S. aureus- or Lactococcus lactis-expressing FnBPB could be activated to the potent serine protease plasmin by staphylokinase and tissue plasminogen activator. Plasminogen bound to recombinant FnBPB with a KD of 0.532 μm as determined by surface plasmon resonance. Plasminogen binding did not to occur by the same mechanism through which FnBPB binds to fibrinogen. Indeed, FnBPB could bind both ligands simultaneously indicating that their binding sites do not overlap. The N3 subdomain of FnBPB contains the full plasminogen-binding site, and this includes, at least in part, two conserved patches of surface-located lysine residues that were recognized by kringle 4 of the host protein., (© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.)
- Published
- 2016
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35. Fibronectin Binding Proteins SpsD and SpsL Both Support Invasion of Canine Epithelial Cells by Staphylococcus pseudintermedius.
- Author
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Pietrocola G, Gianotti V, Richards A, Nobile G, Geoghegan JA, Rindi S, Monk IR, Bordt AS, Foster TJ, Fitzgerald JR, and Speziale P
- Subjects
- Animals, Bacterial Proteins genetics, Cell Line, Cell Wall genetics, Cell Wall metabolism, Dog Diseases metabolism, Dogs, Protein Binding, Staphylococcal Infections metabolism, Staphylococcal Infections microbiology, Staphylococcus genetics, Staphylococcus pathogenicity, Virulence, Bacterial Proteins metabolism, Dog Diseases microbiology, Epithelial Cells microbiology, Fibronectins metabolism, Staphylococcal Infections veterinary, Staphylococcus metabolism
- Abstract
In this study, we investigated the cell wall-anchored fibronectin-binding proteins SpsD and SpsL from the canine commensal and pathogen Staphylococcus pseudintermedius for their role in promoting bacterial invasion of canine progenitor epidermal keratinocytes (CPEK). Invasion was examined by the gentamicin protection assay and fluorescence microscopy. An ΔspsD ΔspsL mutant of strain ED99 had a dramatically reduced capacity to invade CPEK monolayers, while no difference in the invasion level was observed with single mutants. Lactococcus lactis transformed with plasmids expressing SpsD and SpsL promoted invasion, showing that both proteins are important. Soluble fibronectin was required for invasion, and an RGD-containing peptide or antibodies recognizing the integrin α5β1 markedly reduced invasion, suggesting an important role for the integrin in this process. Src kinase inhibitors effectively blocked internalization, suggesting a functional role for the kinase in invasion. In order to identify the minimal fibronectin-binding region of SpsD and SpsL involved in the internalization process, recombinant fragments of both proteins were produced. The SpsD520-846 and SpsL538-823 regions harboring the major fibronectin-binding sites inhibited S. pseudintermedius internalization. Finally, the effects of staphylococcal invasion on the integrity of different cell lines were examined. Because SpsD and SpsL are critical factors for adhesion and invasion, blocking these processes could provide a strategy for future approaches to treating infections., (Copyright © 2015, American Society for Microbiology. All Rights Reserved.)
- Published
- 2015
- Full Text
- View/download PDF
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