Back to Search
Start Over
The immunological and virological consequences of planned treatment interruptions in children with HIV infection
- Source :
- PLoS ONE; Vol 8, PLoS ONE, PLoS One, 8, 10, PLoS ONE, Vol 8, Iss 10, p e76582 (2013), PLoS One, 8
- Publication Year :
- 2013
- Publisher :
- San Francisco, CA : Public Library of Science, 2013.
-
Abstract
- Contains fulltext : 126098.pdf (Publisher’s version ) (Open Access) OBJECTIVES: To evaluate the immunological and viral consequences of planned treatment interruptions (PTI) in children with HIV. DESIGN: This was an immunological and virological sub-study of the Paediatric European Network for Treatment of AIDS (PENTA) 11 trial, which compared CD4-guided PTI of antiretroviral therapy (ART) with continuous therapy (CT) in children. METHODS: HIV-1 RNA and lymphocyte subsets, including CD4 and CD8 cells, were quantified on fresh samples collected during the study; CD45RA, CD45RO and CD31 subpopulations were evaluated in some centres. For 36 (18 PTI, 18 CT) children, immunophenotyping was performed and cell-associated HIV-1 DNA analysed on stored samples to 48 weeks. RESULTS: In the PTI group, CD4 cell count fell rapidly in the first 12 weeks off ART, with decreases in both naive and memory cells. However, the proportion of CD4 cells expressing CD45RA and CD45RO remained constant in both groups. The increase in CD8 cells in the first 12 weeks off ART in the PTI group was predominantly due to increases in RO-expressing cells. PTI was associated with a rapid and sustained increase in CD4 cells expressing Ki67 and HLA-DR, and increased levels of HIV-1 DNA. CONCLUSIONS: PTI in children is associated with rapid changes in CD4 and CD8 cells, likely due to increased cell turnover and immune activation. However, children off treatment may be able to maintain stable levels of naive CD4 cells, at least in proportion to the memory cell pool, which may in part explain the observed excellent CD4 cell recovery with re-introduction of ART.
- Subjects :
- CD31
Genetics and Molecular Biology (all)
CD4-Positive T-Lymphocytes
Time Factors
T-CELL RECONSTITUTION
ACTIVE ANTIRETROVIRAL THERAPY, STRUCTURED TREATMENT INTERRUPTION, T-CELL RECONSTITUTION, HIV-1-INFECTED CHILDREN, IMMUNE RECONSTITUTION, THYMIC OUTPUT, 1-INFECTED CHILDREN
Adolescent
Anti-Retroviral Agents
CD8-Positive T-Lymphocytes
Child
Child, Preschool
Drug Administration Schedule
HIV Infections
Humans
Immunophenotyping
Lymphocyte Count
Treatment Outcome
Viral Load
Agricultural and Biological Sciences (all)
Biochemistry, Genetics and Molecular Biology (all)
Medicine (all)
Biochemistry
law.invention
IMMUNE RECONSTITUTION
0302 clinical medicine
Randomized controlled trial
law
030212 general & internal medicine
HIV-1-INFECTED CHILDREN
0303 health sciences
Multidisciplinary
ACTIVE ANTIRETROVIRAL THERAPY
3. Good health
Medicine
Off Treatment
Poverty-related infectious diseases Infectious diseases and international health [N4i 3]
THYMIC OUTPUT
Viral load
Research Article
Science
1-INFECTED CHILDREN
Auto-immunity, transplantation and immunotherapy [N4i 4]
03 medical and health sciences
Acquired immunodeficiency syndrome (AIDS)
medicine
Preschool
030304 developmental biology
business.industry
medicine.disease
Clinical trial
Immunology
STRUCTURED TREATMENT INTERRUPTION
business
CD8
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Database :
- OpenAIRE
- Journal :
- PLoS ONE; Vol 8, PLoS ONE, PLoS One, 8, 10, PLoS ONE, Vol 8, Iss 10, p e76582 (2013), PLoS One, 8
- Accession number :
- edsair.doi.dedup.....751679ec2c5c90ec90c289bbb4050274