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Abstract Optical wireless communication (OWC) stands out as one of the most promising technologies in the sixth-generation (6G) mobile networks. The establishment of high-quality optical links between transmitters and receivers plays a crucial role in OWC performances. Here, by a compact beam splitter composed of a metasurface and a fiber array, we proposed a wide-angle (~120°) OWC optical link scheme that can parallelly support up to 144 communication users. Utilizing high-speed optical module sources and wavelength division multiplexing technique, we demonstrated each user can achieve a communication speed of 200 Gbps which enables the entire system to support ultra-high communication capacity exceeding 28 Tbps. Furthermore, utilizing the metasurface polarization multiplexing, we implemented a full range wide-angle OWC without blind area nor crosstalk among users. Our OWC scheme simultaneously possesses the advantages of high-speed, wide communication area and multi-user parallel communications, paving the way for revolutionary high-performance OWC in the future.

Asphalt pavement density can be measured using either a destructive or a non-destructive method. The destructive method offers high measurement accuracy but causes damage to the pavement and is inefficient. In contrast, the non-destructive method is highly efficient without damaging the pavement, but its accuracy is not as good as that of the destructive method. Among the devices for non-destructive measurement, the nuclear density gauge (NDG) is the most accurate, but radiation in the device is a serious hazard. The electrical density gauge (EDG), while safer and more convenient to use, is affected by the factors other than density, such as temperature and moisture of the environment. To enhance its accuracy by minimizing or eliminating those non-density factors, an original approach based on artificial neural networks (ANNs) is proposed. Density readings, temperature, and moisture obtained by the EDG are the inputs, and the corresponding densities obtained by the NDG are the outputs to train the ANN models through Levenberg-Marquardt, Bayesian regularization, and Scaled Conjugate Gradient algorithms. Results indicate that the ANN models trained greatly improve the measurement accuracy of the electrical density gauge.

IntroductionIt is unknown how intestinal B cell populations and B cell receptor (BCR) repertoires are established and maintained over time in humans. Following intestinal transplantation (ITx), surveillance ileal mucosal biopsies provide a unique opportunity to map the dynamic establishment of recipient gut lymphocyte populations in immunosuppressed conditions.MethodsUsing polychromatic flow cytometry that includes HLA allele group-specific antibodies distinguishing donor from recipient cells along with high throughput BCR sequencing, we tracked the establishment of recipient B cell populations and BCR repertoire in the allograft mucosa of ITx recipients.ResultsWe confirm the early presence of naïve donor B cells in the circulation (donor age range: 1-14 years, median: 3 years) and, for the first time, document the establishment of recipient B cell populations, including B resident memory cells, in the intestinal allograft mucosa (recipient age range at the time of transplant: 1-44 years, median: 3 years). Recipient B cell repopulation of the allograft was most rapid in infant (

The pollination characteristics and flowering phenology of Calanthe sieboldii were evaluated to elucidate its reproductive characteristics and breeding systems. Field observations and artificial pollination experiments were conducted to study the pollination biology in Xuancheng City, Anhui Province. Meanwhile, gas chromatography–mass spectrometry (GC-MS) was employed to analyze the species’ volatile organic compounds (VOCs). Key findings include the following: (1) the flowering period extends from mid-April to mid-May, with a population-level flowering duration of 29 days in 2017, individual plant flowering averaging 20.22 days, and single flower longevity ranging from 12 to 23 days (mean = 19.30 days); (2) the species exhibits deceptive nectar guides devoid of nectar, indicating food-deceptive pollination, with Bombus sp. identified as its primary pollinator; (3) the pollinial–ovule ratio and hybridization index suggest a high level of self-compatibility without autonomous self-pollination, with no significant difference in pollination success between self- and outcross populations; (4) GC-MS analysis identified methyl benzoate and acacia-related compounds as the primary VOCs of C. sieboldii. These findings provide valuable insights into the conservation and sustainable management of orchids, particularly C. sieboldii.

Bovine mastitis is an inflammatory disease of the mammary glands, and its pathogenesis and diagnosis are complicated. Through qualitative and quantitative analysis of small-molecule metabolites, the metabolomics technique plays an important role in finding biomarkers and studying the metabolic mechanism of bovine mastitis. Therefore, this paper reviews the predictive and diagnostic biomarkers of bovine mastitis that have been identified using metabolomics techniques and that are present in samples such as milk, blood, urine, rumen fluid, feces, and mammary tissue. In addition, the metabolic pathways of mastitis-related biomarkers in milk and blood were analyzed; it was found that the tricarboxylic acid (TCA) cycle was the most significant (FDR = 0.0015767) pathway in milk fluid, and glyoxylate and dicarboxylate metabolism was the most significant (FDR = 0.0081994) pathway in blood. The purpose of this review is to provide useful information for the prediction and early diagnosis of bovine mastitis.

Objective Comprehensive analysis of fracture geometries and seepage characteristics is one of the prominent components in site suitability assessment of high-level radioactive waste (HLW) disposal repositories. Methods To provide a sufficient basis for the construction of the Xinchang underground research laboratory (URL) site in the Beishan preselected area of the HLW disposal repository in China, this paper analyzed the development and distribution of fractures and the seepage characteristics in fractured granite. Based on the borehole tests of BS32, BS36 and BS39 carried out in the middle of the Xinchang site and the field investigation of the surrounding fractures, the orientations and linear density of fractures were used to quantitatively classify the homogeneous zones of the BS32, BS36 and BS39 boreholes. Supplemented by the theory of the hydraulic conductivity tensor, the hydraulic conductivities of fractured rock and the principal directions of fluid seepage in fractured rock with different burial depths were obtained. Results The results show that four groups of dominant fractures are developed around BS32, BS36 and BS39 at the Xinchang URL site, with orientations of 279°∠79°, 98°∠76°, 227°∠79° and 36°∠76°, which are especially dominant in the EW and NNE directions. The fractures are mainly shear-stress formed with steep dips (>60°), accompanied by a few tensile fractures, and the fractures are normally distributed. Compared with the field hydraulic test results, the overall comprehensive hydraulic conductivities of the boreholes are in the range of 10-13-10-9 m/s. The main seepage directions are NNE, nearly EW and SE, where NNE and nearly EW are the dominant seepage channels of the fractured rock mass, with larger main permeability tensor values. Two nearly E-W-trending F6 and F7 faults and their corresponding NNE-E-trending secondary faults play a macrocontrolling role in fracture development at the Xinchang site. The permeability of fractured rock is mainly affected by the fracture spacing and aperture, demonstrating a great anisotropy. Conclusion The results can provide necessary data support for the construction of disposal repositories and the numerical simulation of nuclide migration at the Xinchang URL site. In addition, the presented research idea could provide an alternative and practical method for effectively studying the properties of deep fractured rock masses with deep geological disposal of HLW.

Migratory pests are sudden outbreaks and widespread, putting national food security at risk. Entomological radar is most effective segment in monitoring insect migration, providing critical information for early warning and pest control. Traditional entomological radar can measure biological parameters such as mass and orientation using a low-resolution waveform and rotating linear polarization antenna. The new entomological radar uses a stepped chirp high-resolution waveform and instantaneous fully polarimetric system, which can considerably improve the accuracy of measuring insect biological data. However, in addition to the traditional polarization measurement errors, the stepped chirp waveform introduces new multiplicative error components to different polarization channels, resulting in a more complex imbalance between polarization channels, which requires high-precision polarization calibration. In response to the above issues, the fully polarimetric measurement model is optimized according to the characteristics of the high-resolution system, and a high-resolution system polarization error estimation method based on a sphere and a wire is proposed in this study, which can complete polarization calibration under the loose constraint of the calibrator attitude and compensate for the influence of channel inconsistency on polarization information measurement; additionally, an insect orientation estimation method based on a biological symmetry model is proposed, and the mechanism of cross-talk between polarization channels on orientation estimation is analyzed analytically. Finally, multifrequency high-resolution fully polarimetric radar (X, Ku, Ka) is used for polarimetric calibration and insect orientation measurement experiments, and the measurement error of orientation is less than 3°, which verified the feasibility and effectiveness of the proposed method.

In order to improve the accuracy of vehicle trajectories and ensure driving safety, and considering the differences in driver behavior and the impact of these differences on vehicle trajectories, a vehicle trajectory-prediction method (DBC-Informer) based on the categorization of driver behavior is proposed: firstly, the characteristic driver feature data are extracted through data preprocessing; secondly, descriptive statistical data are obtained through the classification of the driver’s behavior into categories; finally, based on the Informer model, a two-layer driver category trajectory-prediction network architecture is established, which inputs the vehicle trajectories of different driving types into independent prediction sub-networks, respectively, to realize the accurate prediction of vehicle trajectories. The experimental results show that the MAE and MSE values of trajectory prediction of the DBC-Informer model in different time domains are much smaller than those of other comparative models, and the improvement of accuracy is more obvious in the long-term domain trajectory-prediction task scenario, and the increase in prediction error of the DBC-Informer model is significantly reduced after the prediction time exceeds 1 s. The on-line behavioral categorization is achieved by comparing different categorization models; it reaches 98% in classification accuracy and, according to the results of ablation experiments, the addition of the driver behavior-classification method to the prediction model improves the accuracy of prediction in longitudinal and lateral motion by 56% and 61%, respectively, which verifies the effectiveness of the driver behavior-classification method. It can be seen that the DBC-Informer model can more accurately portray the effects of different driving behaviors on vehicle trajectories and improve the accuracy of vehicle trajectory prediction, which provides important data support for vehicle warning systems.

Measuring the orientation, mass and body length of migratory insects through entomological radar is crucial for early warnings of migratory pests. The fully polarized entomological radar is an efficient device for observing migratory insects by calculating insect parameters through the scattering matrix obtained from the target. However, the measured target scattering matrix will be affected by system polarization measurement errors, leading to errors in insect parameter calculation, while the related analysis is currently relatively limited. Therefore, the scattering matrix measurement process is modeled, followed by an analysis of the effects of different errors on orientation, mass and body length estimation. The influence of polarization measurement errors on insect scattering characteristics is also analyzed. The results present that for fixed polarization measurement errors, the measurement errors of insect orientation, mass and body length will vary with insect orientation in specific patterns, and the distribution of measured insect parameters will be drastically distorted compared to the true parameter distribution. In addition, polarization measurement errors could seriously disrupt the reciprocity and bilateral symmetry of the measured insect scattering matrix. These analyses and conclusions provide a good basis for eliminating the effects of polarization measurement errors and improving the accuracy of insect parameter measurement.

Parabens (PBs), a class of endocrine-disrupting chemicals (EDCs), are extensively used as additives in personal care products (PCPs); however, distinguishing between endogenous and exogenous contamination from PCPs in hair remains a challenge. We conducted a comprehensive analysis of the levels, distribution patterns, impact factors, and sources of PBs in 119 human hair samples collected from Changchun, northeast China. The detection rates of methylparaben (MeP), propylparaben (PrP), and ethylparaben (EtP) in hair samples were found to be 100%. The concentration of PBs in hair followed the order of MeP (57.48 ng/g) > PrP (46.40 ng/g) > EtP (6.80 ng/g). The concentration of PrP in female hair was significantly higher (65.38 ng/g) than that observed in male hair (7.82 ng/g) (p

The pursuit of an advanced functional coating that simultaneously combines high hardness, wear resistance, and superior electrical conductivity has remained an elusive goal in the field of copper alloy surface enhancement. Traditional solid solution alloying methods often lead to a significant increase in electron scattering, resulting in a notable reduction in electrical conductivity, making it challenging to achieve a balance between high hardness, wear resistance, and high conductivity. The key lies in identifying a suitable microstructure where dislocation motion is effectively hindered while minimizing the scattering of conductive electrons. In this study, a novel Cu-MoSi2 coating was successfully fabricated on a CuCrZr alloy surface using the coaxial powder feeding high-speed laser cladding technique, with the addition of 10–30% MoSi2 particles. The coating significantly enhances the hardness and wear resistance of the copper substrate while maintaining favorable electrical conductivity. As the quantity of MoSi2 particles increases, the coating’s hardness and wear resistance gradually improve, with minimal variance in conductivity. Among the coatings, the Cu-30%MoSi2 coating stands out with the highest hardness (974.5 HV0.5) and the lowest wear amount (0.062 mg/km), approximately 15 times the hardness of the copper base material (65 HV0.5) and only 0.45% of the wear amount (13.71 mg/km). Additionally, the coating exhibits a resistivity of 0.173 × 10−6 Ω·m. The extraordinary hardness and wear resistance of these coatings can be attributed to the dispersion strengthening effect of MoxSiy particles, while the high electrical conductivity is due to the low silicon content dissolved into the copper from the released MoSi2 particles, as well as the rapid cooling rates associated with the high-speed laser cladding process.

Up till now, measurement of the biological parameters of insects traversing the beam of an entomological radar has been based on the assumption that the insects maintain a horizontal flight attitude. However, recent studies have revealed that some migrating insects fly with their bodies pitched upwards. This article analyzes the influence of nonhorizontal orientation (i.e., pitch and roll angles) on measurements of insect biological parameters with an entomological radar. The scattering matrices (SMs) of 15 insect specimens have been measured at different pitch and roll angles with a purpose-designed multiaspect fully polarimetric laboratory rig. It is found that pitch angle has little influence on the discrimination of “parallel” and “perpendicular” insects, but it affects the measurement accuracy of orientation and estimation of mass and body length. The roll angle has little influence on the insect class discrimination, and the measurement of orientation and body length, and slightly affects the accuracy of the mass estimation. The influence of a general combination of pitch and roll angles is approximately the linear superposition of the influence of pitch angle and roll angle.

Presently, plasmonic sensors are widely employed for the detection of numerous biomolecules found in the human body, owing to their high sensitivity and selectivity even in complex media (e.g., serum, urine, saliva). The most advanced plasmonic sensing technique for real-time screening and monitoring of analyte interactions is surface plasmon resonance (SPR)/localized surface plasmon resonance (LSPR). In recent years, the advancement of signal amplification tags such as gold/silver thin films (nanoparticles) and diverse sensing substrates such as 2D materials (e.g., graphene/MXene) has accelerated the development of SPR/LSPR sensors. This review of the literature focuses on 2D materials-based plasmonic sensors for the detection of various biomolecules, including creatinine, cardiac troponin I, alanine aminotransferase, acetylcholine, cholesterol, glucose, uric acid, and p-cresol. The review concludes with an overview of the advantages and disadvantages of 2D diagnostic materials.

This paper improves the performance of the model by Graph Convolutional Network (GCN) and Firefly Algorithm (FA) to optimize the financial investment risk prediction model. It studies the application of GCN in financial investment risk prediction model and elaborates on the role of FA in the model. To further improve the accuracy of the prediction model, this paper optimizes and improves the FA and verifies the effectiveness of the optimized model through experiments. Experimental results show that the optimized model performs well in feature selection, and the optimal accuracy of feature selection reaches 91.9%, which is much higher than that of traditional models. Meanwhile, in the analysis of the number of iterations of the model, the performance of the optimized algorithm gradually tends to be stable. When the number of iterations is 30, the optimal value is found. In the simulation experiment, when an unexpected accident occurs, the prediction accuracy of the model decreases, but the prediction performance of the optimized algorithm proposed here is significantly higher than that of the traditional model. In conclusion, the optimized model has high accuracy and reliability in financial investment risk prediction, which provides strong support for financial investment decision-making. This paper has certain reference significance for the optimization of financial investment risk prediction model.

Major environmental and climate changes are driven by changes in socioeconomic development. The problem of food waste, its unsustainable management, and associated environmental consequences are becoming great concerns due to the rapid socioeconomic development in many Chinese provinces. This study assessed the current and future status of food waste and its management, and the associated environmental impact in all Chinese provinces from life cycle perspective. Based on result, food waste in China was estimated as 61.37 Mt in 2020 and could be as high as 100.95–139.82 Mt by 2040, under various socioeconomic pathways. Average per capita food waste has increased by 29.67% from 2016 and Tianjin was found to have the highest per capita food waste. Correspondingly, GHG emission of food waste in China was estimated as 149.25 Mt CO2eq in 2020, and is largely influence by the varieties of food wasted in each province. The environmental burden of food waste is projected to increase by 70%–148% under different socioeconomic development pathways. Anaerobic digestion demands freshwater and may pose challenges for Chinese provinces facing water scarcity. Diverting food waste to anaerobic digestion can reduce GHG emissions of food waste by ∼74% by 2040. China’s transition to sustainable waste management may be impeded and unsustainable economic-wise if many Chinese provinces continue to build on their current food waste management trend. These findings could be pivotal in setting food waste reduction goals at provincial level and devising strategies to ensure active involvement of community in food waste reduction programs.

Early weaning increased the economic benefits of piglets. However, early weaning damages the intestinal barrier of piglets and causes immunological stress. The mechanism by which Hippophae rhamnoides polysaccharide (HRP) alleviates lipopolysaccharide (LPS)-induced intestinal porcine epithelial cells (IPEC-J2) inflammatory damage was investigated using proteomics in our previous studies. In this study we employed RNA-sequencing (RNA-seq) to determine the level and function of differentially expressed genes (DEGs) and further explore the mechanism of the HRP anti-inflammatory and immune process. The differential expression analysis indicated that 3622, 1216, and 2100 DEGs in the IPEC-J2 cells were identified in C vs. L, L vs. H6-L, and C vs. H6-L, respectively. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis foundsix identified pathways related to the immune system. Additionally, we used the Science, Technology, Engineering, and Math (STEM) program to categorize the 3,134 DEGs that were differentially expressed in H2-L, H4-L and H6-L into eight possible expression profiles, in which 612 were clustered into two profiles. The accuracy and consistency of RNA-seq data were validated by the results of qRT-PCR of the nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (NFKB2), MAP kinase interacting serine/threonine kinase 2 (MKNK2), mitogen-activated protein kinase kinase 1 (MAP2K1), mitogen-activated protein kinase kinase kinase 8 (MAP3K8), Ras-related protein R-Ras (RRAS), TNF receptor-associated factor 1 (TRAF1), NF-kappa-B inhibitor alpha (NFKBIA), interleukin 8 (IL8), tumor necrosis factor, alpha-induced protein 3 (TNFAIP3), and transforming growth factor beta-1 (TGFB1). Transcriptome sequencing also indicated that HRP reduced the expression levels of related DEGs and inhibited the activation of the mitogen-activated protein kinase (MAPK)/nuclear factor kappa-B (NF-κB) signaling pathway. Our findings indicate that the application of HRP in piglet diets during the early weaning period can improve intestinal epithelial function and integrity, and relieve intestinal damage, and improve piglet health.

Peripartal cows often experience negative energy balance, and are therefore prone to suffering from metabolic diseases such as hyperketonemia, which causes financial losses in dairy farms. This study aimed to investigate the effect of green tea polyphenol (GTP) supplementation during the periparturient period on production performance, oxidative stress and immunometabolism in dairy cows with hyperketonemia. One hundred Holstein cows were assigned to GTP (0.2 g/kg DM; n = 50) or control (without GTP; n = 50) group based on body weight, previous milk yield, and parity on d 15 before expected parturition. Subsequently, 10 cows with hyperketonemia were selected from each group, according to blood β-hydroxybutyric acid (BHBA) concentration between 1.2 and 2.9 mmol/L from 2 to 3 d postpartum. All cows were fed a close-up diet and a lactation diet with or without GTP supply from 15 d prepartum until 30 d postpartum. Milk and blood samples were obtained from 20 cows selected with hyperketonemia on 10, 20, and 30 d postpartum. Compared with control cows, greater milk yield and lower somatic cell count were observed in GTP cows. The GTP group had lower concentrations of BHBA, free fatty acids, cholesterol, triglyceride, reactive oxygen species, malondialdehyde, and hydrogen peroxide, greater concentrations of glucose, lower activities of aspartate aminotransferase, alanine aminotransferase, and glutamyl transpeptidase, alongside greater activities of superoxide dismutase, glutathione peroxidase, and total antioxidant capacity. Additionally, GTP supplementation up-regulated concentrations of interleukin-6 and interleukin-10, but down-regulated concentrations of tumor necrosis factor-α, interleukin-1β, interleukin-2, interleukin-8, and interferon-γ in plasma. Greater concentrations of plasma immunoglobulin G were also detected in the GTP group. Overall, the data suggested that GTP supplementation from 15 d prepartum to 30 d postpartum improved the milk yield and health status in cows with hyperketonemia during early lactation.

This study evaluated the effects of dietary coated sodium butyrate (CSB) on lipopolysaccharide (LPS)-induced inflammatory response in weaned lambs. A basal diet was supplemented with CSB at 0, 2 or 3 g/kg feed for 28 days and/or intraperitoneally injected with 100 μg/kg BW Escherichia coli LPS (0 g/kg CSB, 0 g/kg CSB and LPS, 2 g/kg CSB and LPS, 3 g/kg CSB and LPS). The results showed that CSB supplementation conferred significant protective effects against LPS-induced inflammatory response by reducing in the levels of D-lactate (DLA), diamine oxidase (DAO), inflammatory cytokines (TNF-α, IL-1β and IL-8) in the serum and upregulated expression of tight junction proteins (claudin-3, ZO-1 and occludin) in the ileum. Meanwhile, CSB pre-treatment significantly suppressed LPS-induced TLR4/NF-κB signalling pathway mRNA expression (MyD88, TLR4, NF-κB, IL-6 and IL-1β) and down-regulated TLR4/NF-κB signalling pathway protein expression (MyD88, TLR4, NF-κB p65 and p-NF-κB p65). Furthermore, dietary CSB supplementation improved intestinal morphology. Lastly, bacterial 16S rRNA gene sequencing indicated that CSB could alter intestinal microbiota composition at genus levels. Taken together, our results indicate CSB could maintain intestinal health, improve intestinal morphology, modulate the microbial community and decrease LPS-induced inflammatory response in lambs.

Abstract Background Network meta-analyses using individual participant data (IPD-NMAs) have been increasingly used to compare the effects of multiple interventions. Although there have been many studies on statistical methods for IPD-NMAs, it is unclear whether there are statistical defects in published IPD-NMAs and whether the reporting of statistical analyses has improved. This study aimed to investigate statistical methods used and assess the reporting and methodological quality of IPD-NMAs. Methods We searched four bibliographic databases to identify published IPD-NMAs. The methodological quality was assessed using AMSTAR-2 and reporting quality assessed based on PRISMA-IPD and PRISMA-NMA. We performed stratified analyses and correlation analyses to explore the factors that might affect quality. Results We identified 21 IPD-NMAs. Only 23.8% of the included IPD-NMAs reported statistical techniques used for missing participant data, 42.9% assessed the consistency, and none assessed the transitivity. None of the included IPD-NMAs reported sources of funding for trials included, only 9.5% stated pre-registration of protocols, and 28.6% assessed the risk of bias in individual studies. For reporting quality, compliance rates were lower than 50.0% for more than half of the items. Less than 15.0% of the IPD-NMAs reported data integrity, presented the network geometry, or clarified risk of bias across studies. IPD-NMAs with statistical or epidemiological authors often better assessed the inconsistency (P = 0.017). IPD-NMAs with a priori protocol were associated with higher reporting quality in terms of search (P = 0.046), data collection process (P = 0.031), and syntheses of results (P = 0.006). Conclusions The reporting of statistical methods and compliance rates of methodological and reporting items of IPD-NMAs were suboptimal. Authors of future IPD-NMAs should address the identified flaws and strictly adhere to methodological and reporting guidelines.

Abstract Objective Little is known about the intrinsic electrophysiological properties of hypothalamic hamartoma (HH) in vivo and seizure network since only few cases using stereoelectroencephalography (SEEG) electrodes exploring both cortex and HH have been published. To elucidate these issues, we analyzed simultaneous SEEG recordings in HH and cortex systematically. Methods We retrospectively investigated data from 15 consecutive patients with SEEG electrodes into the HH for the treatment purpose of radiofrequency thermocoagulation treatment. Additional SEEG electrodes were placed into the cortex in 11 patients to assess extra‐HH involvement. Interictal discharges within the HH and anatomo‐electroclinical correlations during seizures of each patient were qualitatively and quantitatively analyzed. Results Overall, 77 electrodes with 719 contacts were implanted, and 33 spontaneous seizures were recorded during long‐term SEEG monitoring. Interictally, distinct electrophysiological patterns, including isolated intermittent spikes/sharp waves, burst spike and wave trains, paroxysmal fast discharges, periodic discharges, and high‐frequency oscillations, were identified within the HH. Notably, synchronized or independent interictal discharges in the cortex were observed. Regarding the ictal discharges, the electrical onset pattern within the HH always started with abrupt giant shifts superimposed on low‐voltage fast activity across patients. The gelastic seizure network mainly involved the HH, orbitofrontal areas, and cingulate gyrus. Seizures with automatisms and impaired awareness primarily propagated to mesial temporal lobes. Moreover, independent ictal discharges arising from the mesial temporal lobe were detected in three out of nine patients. Interpretation This study comprehensively reveals intrinsic electrophysiological patterns and epileptogenic networks in vivo, providing new insights into the mechanisms underlying cortical and subcortical epileptogenesis.

Oxidative stress (OS) is one of the main limiting factors affecting the length of lactation and milk quality in dairy cows. For high-producing dairy cows, the OS of mammary glands is a serious problem. Green tea polyphenols (GTP), found mainly in tea, are a combination of many phenols. GTP have a good effect on antioxidation, inflammation resistance, obesity, fat cell metabolism improvement, and lowering of blood lipid. Therefore, we studied the role of GTP on OS in dairy cows and further investigated whether GTP alleviates oxidative damage of bovine mammary epithelial cells (BMECs) induced by hydrogen peroxide (H2O2) and its underlying molecular mechanism. In this study, 500 μM of H2O2 for 12 h incubation was chosen as the condition of the OS model of BMECs. In addition, the present results found that treatment with GTP alleviated the oxidative damage induced by H2O2 [the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) were significantly increased, and the contents of malondialdehyde (MDA), 8-isoprostaglandin (8-iso-PG), 8-oxo-deoxyguanosine (8-OHdG), and protein carbonyl (PC) and caspase-3 and caspase-9 activities were significantly reduced]. These effects are related to the activation of the erythrocyte-derived nuclear factor 2-like protein 2 (NFE2L2) signaling pathway and the inactivation of the caspase/Bcl-2 apoptotic pathway. When NFE2L2 short interfering RNA (siRNA) was used to downregulate the expression of NFE2L2 in cultured BMECs, NFE2L2-siRNA transfection abolished the protective effect of GTP on H2O2-induced intracellular reactive oxygen species (ROS) accumulation and apoptosis. In addition, the mitogen-activated protein kinase (MAPK) inhibition test further proved that GTP relieved H2O2-induced oxidative damage by activating the NFE2L2 signaling pathway, which was achieved by activating the extracellular-regulated kinase 1/2 (ERK1/2) signaling pathway. Overall, the results indicate that GTP has a beneficial effect on the redox balance of BMECs. In addition, GTP might be a latent antioxidant in vivo, which can be administered to ruminants during stressful periods such as the perinatal period.

A cold spray–laser cladding composite gradient coating (CLGC) was successfully formed on a Cu substrate. In comparison with traditional laser cladding gradient coatings (LGC), cold spraying the pre-set Ni-Cu alloy’s intermediate transition layer not only mitigates the negative impacts due to the high reflectivity of the copper substrate but also helps to minimize the difference in the coefficients of thermal expansion (CTE) between the substrate and coating. This reduces the overall crack sensitivity and improves the cladding quality of the coating. Besides this, the uniform distribution of hard phases in CLGC, such as Ni11Si12 and Mo5Si3, greatly increases its microhardness compared to the Cu substrate, thus resulting in the value of 478.8 HV0.5 being approximately 8 times that of the Cu substrate. The friction coefficient of CLGC is lowered compared to both the Cu substrate and LGC with respective values of 0.28, 0.54, and 0.43, and its wear rate is only one-third of the Cu substrate’s. These results suggest CLGC has excellent anti-wear properties. In addition, the wear mechanism was determined from the microscopic morphology and element distribution and was found to be oxidative and abrasive. This approach combines cold spraying and laser cladding to form a nickel-based gradient coating on a Cu substrate without cracks, holes, or other faults, thus improving the wear resistance of the Cu substrate and improving its usability.

This article discusses optically active nanomaterials and their optical biosensing applications. In addition to enhancing their sensitivity, these nanomaterials also increase their biocompatibility. For this reason, nanomaterials, particularly those based on their chemical compositions, such as carbon-based nanomaterials, inorganic-based nanomaterials, organic-based nanomaterials, and composite-based nanomaterials for biosensing applications are investigated thoroughly. These nanomaterials are used extensively in the field of fiber optic biosensing to improve response time, detection limit, and nature of specificity. Consequently, this article describes contemporary and application-based research that will be of great use to researchers in the nanomaterial-based optical sensing field. The difficulties encountered during the synthesis, characterization, and application of nanomaterials are also enumerated, and their future prospects are outlined for the reader’s benefit.

Optical fiber sensors based on surface plasma technology have many unique advantages in specific applications such as extreme environmental monitoring, physical parameter determination, and biomedical indicators testing. In recent decades, various kinds of fiber probes with special structures were developed according to special processing such as tapering, splicing, etching, fiber balls, grating etc. In this paper, the fabrication technology, characteristics, development status and application scenarios of different special optical fiber structures are briefly reviewed, including common processing equipment. Furthermore, many special novel optical fiber structures reported in recent years are summarized, which have been used in various kinds of plasmonic sensing work. Then, the fiber-plasmonic sensors for practical applications are also introduced and examined in detail. The main aim of this review is to provide guidance and inspiration for researchers to design and fabricate special optical fiber structures, thus facilitating their further research.

Entomological radar is a specially designed instrument that can measure the behavioral and biological characteristics of high-altitude migrating insects. Its application is of great significance for the monitoring, early warning, and control of agricultural pests. As an important component of the local migratory biomass, insects fly in the air during the day and night. The fully polarimetric entomological radar was carefully designed with all-day, all-weather, and multi-function measurement capabilities. The fully polarimetric entomological radar measures the mass of a single insect based on the radar cross-sectional (RCS) measurement and then calculates the biomass of migrating insects. Therefore, the measurement accuracy of the insect RCS is the key indicator affecting the accuracy of migratory biomass statistics. Due to the radar’s lack of in-beam angle measurement ability, the insect RCS is usually measured based on the assumption that the insect is on the beam center. Therefore, the measured RCS will be smaller than true value if the insect deviates from the beam center due to the gain curve of the antenna. This leads to measurement errors in regard to the insect mass and migratory biomass. In order to solve this problem, a biomass estimation method, reported in this paper, was designed under the assumption of a uniform distribution of migrating insects in the radar monitoring airspace. This method can estimate the individual RCS expectation of migrating insects through a statistical method without measuring the position of the insects in the beam and then obtain the migratory biomass. The effectiveness of the model and algorithm is verified by simulations and entomological radar field measurements.

This article presents a concise summary of current advancements in novel nanomaterial-based optical fiber biosensors. The beneficial optical and biological properties of nanomaterials, such as nanoparticle size-dependent signal amplification, plasmon resonance, and charge-transfer capabilities, are widely used in biosensing applications. Due to the biocompatibility and bioreceptor combination, the nanomaterials enhance the sensitivity, limit of detection, specificity, and response time of sensing probes, as well as the signal-to-noise ratio of fiber optic biosensing platforms. This has established a practical method for improving the performance of fiber optic biosensors. With the aforementioned outstanding nanomaterial properties, the development of fiber optic biosensors has been efficiently promoted. This paper reviews the application of numerous novel nanomaterials in the field of optical fiber biosensing and provides a brief explanation of the fiber sensing mechanism.

Background: Posttraumatic stress disorder (PTSD) and subthreshold PTSD are still major global concerns, especially in developing areas short of mental health resources. Written exposure therapy (WET), a brief 5-session treatment, has been found to be effective in reducing PTSD symptoms, but no studies have examined it in an Eastern context. Mindfulness-based meditation mobile application may be a promising approach to reduce insomnia comorbid with PTSD. The current study aims to: 1) examine the effectiveness of WET for Chinese PTSD and subthreshold PTSD patients, and 2) examine the effectiveness of adding a mindfulness-based application (MBA) to WET for reducing comorbid insomnia. Methods: The randomized controlled trial will enroll 150 adults with subthreshold/full PTSD and comorbid insomnia. Participants will be randomly assigned to written exposure therapy plus mindfulness-based application condition (WET + MBA, n = 50), written exposure therapy alone (WET, n = 50), or minimal contact control (MMC, n = 50). Clinical interview of the primary outcome (PTSD symptoms) will be administrated at baseline, posttreatment, 3- and 6- month follow-up, while self-reported PTSD symptoms and secondary outcomes (insomnia severity) will be administrated at baseline, every week and all follow-ups. Discussion: This is the first study applying WET in Chinese PTSD patients, as well as examining a mindfulness-based mobile application as a treatment add-on for comorbid insomnia. Study findings will contribute to the knowledge of the effectiveness of WET and a mindfulness-based mobile application, and the development of a culture-adapted treatment protocol. Trial registration: ChiCTR, ChiCTR2000034119. Registered 24 June 2020, http://www.chictr.org.cn/showproj.aspx?proj=55,467.

Measuring migration flux with entomological radar is of great importance to assess the biomass of migratory insects and study the influence of insects on the ecosystem. However, the migration flux is measured with a large quantity of errors for the entomological radar without the ability of in-beam angle measurement, because the insect RCS is measured with the assumption that the insect flies over the beam center. When the insect does not pass through the beam center, the measured RCS is less than the true value. To improve the estimation accuracy of migration flux, a new estimation method of migration flux based on statistical hypothesis is proposed for radars working in the fixed-beam vertical-looking mode. This method avoids the RCS measurement error caused by the offset of the insect trajectory to the radar beam center by assuming that the insect flight trajectory is evenly distributed in the beam and calculating the average value of flux. This method is extended to be used in fixed-beam arbitrary pointing mode and a new proposed scanning mode. The effectiveness of the proposed method is verified by simulations and migration insect data measured by a radar.

For entomological radar, the polarization information of the target is usually used to estimate the biological parameters, such as orientation, body length, and mass, of the insect. Thus, the accuracy of polarization measurement directly affects the performance of the biological parameters’ estimation. The polarization measurement error is mainly caused by the imbalance of amplitude and phase between two polarization channels and the crosstalk of the dual-polarization antenna. In order to obtain the correct polarization information of the target, the polarimetric calibration of the entomological radar is required. This paper proposes a new polarimetric calibration technique based on antenna rotation, which does not require the calibrator to have a specific polarization scattering matrix (PSM). Compared with the currently existing calibration techniques, no prior knowledge of the calibrator PSM is required (in fact, any fixed-point target can be used as a calibrator); thus, the errors introduced by the mechanical process can be avoided. Simulations and data measured by radar verify the effectiveness of the method. This method has the potential to be extended to other fully polarimetric radar systems in the future, such as fully polarimetric weather radar, fully polarimetric synthetic aperture radar (SAR), and so on.

IntroductionLaryngeal mask airway (LMA), an alternative to traditional tracheal intubation, is widely used in clinical practice and is considered to be an effective device for airway management. LMA and i-gel have been widely used in anaesthesia and emergency situations in children. Some systematic reviews have evaluated the efficacy of LMA and i-gel in children, but they have not shown consistent results in clinical performance. This study aims to evaluate the airway complications of all subtypes of LMA and i-gel in child patients under general anaesthesia using a Bayesian network meta-analysis (NMA).Methods and analysisPubMed, EMBASE.com, the Cochrane library, Web of Science and Chinese Biomedical Literature Database will be searched from inception to January 2019. We will include prospective randomised controlled trials (RCTs) that reported the subtypes of LMA and i-gel regardless of sample size. The risk of bias assessment of the included RCTs will be conducted according to the Cochrane Handbook V.5.1.0. A Bayesian NMA will be performed using WinBUGS V.1.4.3. Grading of Recommendations Assessment, Development and Evaluation will be used to explore the quality of evidence.Ethics and disseminationEthics approval and patient consent are not required as this study is an NMA based on published trials. The results of this NMA will be submitted to a peer-reviewed journal for publication.PROSPERO registration numberCRD42019127668.

Herpesvirus-associated ubiquitin-specific protease (HAUSP, also known as USP7), a deubiquitylating enzyme of the ubiquitin-specific processing protease family, specifically deubiquitylates both p53 and MDM2, hence playing an important yet enigmatic role in the p53-MDM2 pathway. Here we demonstrate that both p53 and MDM2 specifically recognize the N-terminal tumor necrosis factor-receptor associated factor (TRAF)-like domain of HAUSP in a mutually exclusive manner. HAUSP preferentially forms a stable HAUSP-MDM2 complex even in the presence of excess p53. The HAUSP-binding elements were mapped to a peptide fragment in the carboxy-terminus of p53 and to a short-peptide region preceding the acidic domain of MDM2. The crystal structures of the HAUSP TRAF-like domain in complex with p53 and MDM2 peptides, determined at 2.3-A and 1.7-A resolutions, respectively, reveal that the MDM2 peptide recognizes the same surface groove in HAUSP as that recognized by p53 but mediates more extensive interactions. Structural comparison led to the identification of a consensus peptide-recognition sequence by HAUSP. These results, together with the structure of a combined substrate-binding-and-deubiquitylation domain of HAUSP, provide important insights into regulation of the p53-MDM2 pathway by HAUSP.

Introduction: It is unknown how intestinal B cell populations and B cell receptor (BCR) repertoires are established and maintained over time in humans. Following intestinal transplantation (ITx), surveillance ileal mucosal biopsies provide a unique opportunity to map the dynamic establishment of recipient gut lymphocyte populations in immunosuppressed conditions. Methods: Using polychromatic flow cytometry that includes HLA allele groupspecific antibodies distinguishing donor from recipient cells along with high throughput BCR sequencing, we tracked the establishment of recipient B cell populations and BCR repertoire in the allograft mucosa of ITx recipients. Results: We confirm the early presence of naïve donor B cells in the circulation (donor age range: 1-14 years, median: 3 years) and, for the first time, document the establishment of recipient B cell populations, including B resident memory cells, in the intestinal allograft mucosa (recipient age range at the time of transplant: 1-44 years, median: 3 years). Recipient B cell repopulation of the allograft was most rapid in infant (<1 year old)-derived allografts and, unlike T cell repopulation, did not correlate with rejection rates. While recipient memory B cell populations were increased in graft mucosa compared to circulation, naïve recipient B cells remained detectable in the graft mucosa for years. Comparisons of peripheral and intra-mucosal B cell repertoires in the absence of rejection (recipient age range at the time of transplant: 1-9 years, median: 2 years) revealed increased BCR mutation rates and clonal expansion in graft mucosa compared to circulating B cells, but these parameters did not increase markedly after the first year post-transplant. Furthermore, clonal mixing between the allograft mucosa and the circulation was significantly greater in ITx recipients, even years after transplantation, than in deceased adult donors. In available pan-scope biopsies from pediatric recipients, we observed higher percentages of naïve recipient B cells in colon allograft compared to small bowel allograft and increased BCR overlap between native colon vs colon allograft compared to that between native colon vs ileum allograft in most cases, suggesting differential clonal distribution in large intestine vs small intestine. Discussion: Collectively, our data demonstrate intestinal mucosal B cell repertoire establishment from a circulating pool, a process that continues for years without evidence of stabilization of the mucosal B cell repertoire in pediatric ITx patients. [ABSTRACT FROM AUTHOR]