139 results on '"Martínez-Andújar, Cristina"'
Search Results
2. Salicylic Acid and Calcium Chloride Seed Priming: A Prominent Frontier in Inducing Mineral Nutrition Balance and Antioxidant System Capacity to Enhance the Tolerance of Barley Plants to Salinity
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Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Ben Youssef, Rim, Jelali, Nahida, Martínez-Andújar, Cristina, Abdelly, Chedly, Hernández, José Antonio, Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Ben Youssef, Rim, Jelali, Nahida, Martínez-Andújar, Cristina, Abdelly, Chedly, and Hernández, José Antonio
- Abstract
The current investigation aims to underline the impact of salicylic acid or calcium chloride seed pre-treatments on mineral status and oxidative stress markers, namely levels of electrolyte leakage (EL) and lipid peroxidation levels, measured as thiobarbituric reactive substances (TBARS), and the activity of some antioxidant enzymes in roots and leaves of plants in two barley species grown under various salt treatments. Overall, our results revealed that salinity inhibits essential nutrient absorption such as iron, calcium, magnesium and potassium and stimulates the absorption of sodium. Also, this environmental constraint induced oxidative stress in plants in comparison with the control conditions. This state of oxidative stress is reflected by an increase in TBARS content as well as the stimulation of EL values. In addition, salinity induced disturbances in the activity of antioxidant enzymes, which were mainly dependent on the applied salt concentration and the species. In addition, Hordeum marinum maintained high antioxidant enzyme activity and low levels of oxidative stress parameters, which reinforces its salt-tolerant character. Importantly, salicylic acid or calcium chloride seed priming alleviated the mineral imbalance and the oxidative damage induced by salinity. Moreover, seed priming improves iron, calcium magnesium and potassium content and limitsthe accumulation of sodium. Also, both treatments not only decrease TBARS levels and limit EL, but they also stimulate the antioxidant enzyme activities in the leaves and roots of the stressed plants as compared with stressed plants grown from non-primed seeds. Interestingly, the beneficial effects of the mentioned treatments were more notable on Hordeum vulgare species.
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- 2024
3. Salicylic Acid and Calcium Chloride Seed Priming: A Prominent Frontier in Inducing Mineral Nutrition Balance and Antioxidant System Capacity to Enhance the Tolerance of Barley Plants to Salinity.
- Author
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Ben Youssef, Rim, Jelali, Nahida, Martínez-Andújar, Cristina, Abdelly, Chedly, and Hernández, José Antonio
- Subjects
SALICYLIC acid ,CALCIUM chloride ,ACYL chlorides ,OXIDANT status ,MINERALS in nutrition ,HORDEUM ,GERMINATION ,BARLEY - Abstract
The current investigation aims to underline the impact of salicylic acid or calcium chloride seed pre-treatments on mineral status and oxidative stress markers, namely levels of electrolyte leakage (EL) and lipid peroxidation levels, measured as thiobarbituric reactive substances (TBARS), and the activity of some antioxidant enzymes in roots and leaves of plants in two barley species grown under various salt treatments. Overall, our results revealed that salinity inhibits essential nutrient absorption such as iron, calcium, magnesium and potassium and stimulates the absorption of sodium. Also, this environmental constraint induced oxidative stress in plants in comparison with the control conditions. This state of oxidative stress is reflected by an increase in TBARS content as well as the stimulation of EL values. In addition, salinity induced disturbances in the activity of antioxidant enzymes, which were mainly dependent on the applied salt concentration and the species. In addition, Hordeum marinum maintained high antioxidant enzyme activity and low levels of oxidative stress parameters, which reinforces its salt-tolerant character. Importantly, salicylic acid or calcium chloride seed priming alleviated the mineral imbalance and the oxidative damage induced by salinity. Moreover, seed priming improves iron, calcium magnesium and potassium content and limitsthe accumulation of sodium. Also, both treatments not only decrease TBARS levels and limit EL, but they also stimulate the antioxidant enzyme activities in the leaves and roots of the stressed plants as compared with stressed plants grown from non-primed seeds. Interestingly, the beneficial effects of the mentioned treatments were more notable on Hordeum vulgare species. [ABSTRACT FROM AUTHOR]
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- 2024
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4. Protective Effect (Safener) of Melatonin on Vigna Radiata L. Seedlings in the Presence of the Fungicide Copper Oxychloride
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Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), Martínez-Andújar, Cristina [0000-0002-3684-9765], Giraldo-Acosta, Manuela, Martínez-Andújar, Cristina, Martínez-Melgarejo, Purificación A., Cano, Antonio, Hernández-Ruiz, Josefa, Arnao, Marino B., Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), Martínez-Andújar, Cristina [0000-0002-3684-9765], Giraldo-Acosta, Manuela, Martínez-Andújar, Cristina, Martínez-Melgarejo, Purificación A., Cano, Antonio, Hernández-Ruiz, Josefa, and Arnao, Marino B.
- Abstract
Melatonin (N-acetyl-5-methoxytriptamine) is a novel plant regulatory molecule currently under study. Its role as a biostimulator and protector against abiotic and biotic stressors, through the regulation of the redox network and change in the expression of many elements of primary and secondary metabolism, is of great interest. The possible protective effect of melatonin in mungbean seedlings, previously primed seed treated with the fungicide copper oxychloride, was studied. The effect of melatonin and fungicide in growth of seedlings and photosynthetic pigments, leakage membranes, lipid peroxidation, antioxidant activity, and phytomelatonin content was studied. Also, the effect of exogenous melatonin on endogenous plant hormones indoleacetic acid, gibberellins, cytokinins, abscisic acid, salicylic acid, and jasmonic acid levels, in the absence and presence of fungicide, was analyzed. Melatonin improved growth of roots and aerial parts in the presence of fungicide; chlorophyll and carotenoid contents were protected by melatonin in the presence of melatonin and in melatonin-fungicide co-treatments. Membrane damage due to fungicide was lessened by melatonin. The hormonal profile (auxin, gibberellins, cytokinins, abscisic acid, ethylene precursor, salicylic acid, and jasmonic acid) in roots and leaves was greatly affected by copper fungicide and melatonin treatments. In general, an increasing in plant tolerance response has been detected, proposing melatonin as a natural safener molecule of plants in the presence of copper fungicide
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- 2023
5. Changing biosynthesis of terpenoid percursors in rice through synthetic biology
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Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), European Commission, Ministerio de Economía y Competitividad (España), Albacete, Alfonso [0000-0003-1332-8593], Martínez-Andújar, Cristina [0000-0002-3684-9765], Basallo, Oriol, Pérez, Lucía, Lucido, Abel, Sorribas, Albert, Marin-Saguino, Alberto, Vilaprinyó, Ester, Pérez-Fons, Laura, Albacete, Alfonso, Martínez-Andújar, Cristina, Fraser, Paul D., Christou, Paul, Capell, Teresa, Alves, Rui, Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), European Commission, Ministerio de Economía y Competitividad (España), Albacete, Alfonso [0000-0003-1332-8593], Martínez-Andújar, Cristina [0000-0002-3684-9765], Basallo, Oriol, Pérez, Lucía, Lucido, Abel, Sorribas, Albert, Marin-Saguino, Alberto, Vilaprinyó, Ester, Pérez-Fons, Laura, Albacete, Alfonso, Martínez-Andújar, Cristina, Fraser, Paul D., Christou, Paul, Capell, Teresa, and Alves, Rui
- Abstract
Many highly valued chemicals in the pharmaceutical, biotechnological, cosmetic, and biomedical industries belong to the terpenoid family. Biosynthesis of these chemicals relies on polymerization of Isopentenyl di-phosphate (IPP) and/or dimethylallyl diphosphate (DMAPP) monomers, which plants synthesize using two alternative pathways: a cytosolic mevalonic acid (MVA) pathway and a plastidic methyleritritol-4-phosphate (MEP) pathway. As such, developing plants for use as a platform to use IPP/DMAPP and produce high value terpenoids is an important biotechnological goal. Still, IPP/DMAPP are the precursors to many plant developmental hormones. This creates severe challenges in redirecting IPP/DMAPP towards production of non-cognate plant metabolites. A potential solution to this problem is increasing the IPP/DMAPP production flux in planta. Here, we aimed at discovering, understanding, and predicting the effects of increasing IPP/DMAPP production in plants through modelling. We used synthetic biology to create rice lines containing an additional ectopic MVA biosynthetic pathway for producing IPP/DMAPP. The rice lines express three alternative versions of the additional MVA pathway in the plastid, in addition to the normal endogenous pathways. We collected data for changes in macroscopic and molecular phenotypes, gene expression, isoprenoid content, and hormone abundance in those lines. To integrate the molecular and macroscopic data and develop a more in depth understanding of the effects of engineering the exogenous pathway in the mutant rice lines, we developed and analyzed data-centric, line-specific, multilevel mathematical models. These models connect the effects of variations in hormones and gene expression to changes in macroscopic plant phenotype and metabolite concentrations within the MVA and MEP pathways of WT and mutant rice lines. Our models allow us to predict how an exogenous IPP/DMAPP biosynthetic pathway affects the flux of terpenoid precursors. We als
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- 2023
6. Changing biosynthesis of terpenoid percursors in rice through synthetic biology
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Basallo, Orio, primary, Perez, Lucia, additional, Lucido, Abel, additional, Sorribas, Albert, additional, Marin-Saguino, Alberto, additional, Vilaprinyo, Ester, additional, Perez-Fons, Laura, additional, Albacete, Alfonso, additional, Martínez-Andújar, Cristina, additional, Fraser, Paul D., additional, Christou, Paul, additional, Capell, Teresa, additional, and Alves, Rui, additional
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- 2023
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7. Overexpression of TgERF1, a Transcription Factor from Tectona grandis, Increases Tolerance to Drought and Salt Stress in Tobacco
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Oliveira, Perla Novais de, primary, Matias, Fernando, additional, Martínez-Andújar, Cristina, additional, Martinez-Melgarejo, Purificación Andrea, additional, Prudencio, Ángela Sánchez, additional, Galeano, Esteban, additional, Pérez-Alfocea, Francisco, additional, and Carrer, Helaine, additional
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- 2023
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8. Overexpression of the vascular brassinosteroid receptor BRL3 confers drought resistance without penalizing plant growth
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Fàbregas, Norma, Lozano-Elena, Fidel, Blasco-Escámez, David, Tohge, Takayuki, Martínez-Andújar, Cristina, Albacete, Alfonso, Osorio, Sonia, Bustamante, Mariana, Riechmann, José Luis, Nomura, Takahito, Yokota, Takao, Conesa, Ana, Alfocea, Francisco Pérez, Fernie, Alisdair R., Caño-Delgado, Ana I., Fàbregas, Norma, Lozano-Elena, Fidel, Blasco-Escámez, David, Tohge, Takayuki, Martínez-Andújar, Cristina, Albacete, Alfonso, Osorio, Sonia, Bustamante, Mariana, Riechmann, José Luis, Nomura, Takahito, Yokota, Takao, Conesa, Ana, Alfocea, Francisco Pérez, Fernie, Alisdair R., and Caño-Delgado, Ana I.
- Abstract
Drought represents a major threat to food security. Mechanistic data describing plantresponses to drought have been studied extensively and genes conferring drought resistancehave been introduced into crop plants. However, plants with enhanced drought resistanceusually display lower growth, highlighting the need for strategies to uncouple droughtresistance from growth. Here, we show that overexpression of BRL3, a vascular-enrichedmember of the brassinosteroid receptor family, can confer drought stress tolerance inArabidopsis. Whereas loss-of-function mutations in the ubiquitously expressed BRI1 receptorleads to drought resistance at the expense of growth, overexpression of BRL3 receptorconfers drought tolerance without penalizing overall growth. Systematic analyses reveal thatupon drought stress, increased BRL3 triggers the accumulation of osmoprotectant metabo-lites including proline and sugars. Transcriptomic analysis suggests that this results fromdifferential expression of genes in the vascular tissues. Altogether, this data suggests thatmanipulating BRL3 expression could be used to engineer drought tolerant crops.
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- 2023
9. Bumblebees sense rootstock-mediated nutrition and fertilization regime in tomato
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Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), European Commission, Pérez-Alfocea, Francisco [0000-0003-1057-4924], Martínez-Andújar, Cristina, Ben Youssef, Rim, Prudencio, Ángela S., Ormazabal, Maialen, Martín-Rodríguez, A.J., Albacete, Alfonso, Martínez-Melgarejo, Purificación A., Pérez-Alfocea, Francisco, Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), European Commission, Pérez-Alfocea, Francisco [0000-0003-1057-4924], Martínez-Andújar, Cristina, Ben Youssef, Rim, Prudencio, Ángela S., Ormazabal, Maialen, Martín-Rodríguez, A.J., Albacete, Alfonso, Martínez-Melgarejo, Purificación A., and Pérez-Alfocea, Francisco
- Abstract
Since producing more with less is required for increasing agricultural sustainability and reducing its environmental impact, breeding varieties with increased yield stability under reduced fertilizer application is an important goal, particularly in high valued horticultural crops such as tomato (Solanum lycopersicum L.). However, because of the difficulties to conciliate yield and fertilizer use efficiency through breeding, the graft-compatible genetic biodiversity existing in horticultural species offers the possibility to directly approach this objective in high-yielding elite varieties through improving nutrient capture and promoting ecosystem services such as insect pollination. We hypothesized that rootstocks affect pollinator foraging decisions through the nutritional status that impacts yield
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- 2023
10. Overexpression of the vascular brassinosteroid receptor BRL3 confers drought resistance without penalizing plant growth
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Fàbregas, Norma, Lozano-Elena, Fidel, Blasco-Escámez, David, Tohge, Takayuki, Martínez-Andújar, Cristina, Albacete, Alfonso, Osorio, Sonia, Bustamante, Mariana, Riechmann, José Luis, Nomura, Takahito, Yokota, Takao, Conesa, Ana, Alfocea, Francisco Pérez, Fernie, Alisdair R., and Caño-Delgado, Ana I.
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- 2018
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11. Bumblebees sense rootstock‑mediated nutrition and fertilization regime in tomato
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Martínez‑Andújar, Cristina, Ben Youssef, Rim, Prudencio, Ángela S., Ormazabal, Maialen, Martin-Rodriguez, José Angel, Albacete, Alfonso, Martínez‑Melgarejo, Purifcación, and Pérez‑Alfocea, Francisco
- Abstract
Aims Since producing more with less is required for increasing agricultural sustainability and reducing its environmental impact, breeding varieties with increased yield stability under reduced fertilizer application is an important goal, particularly in high valued horticultural crops such as tomato (Solanum lycopersicum L.). However, because of the difficulties to conciliate yield and fertilizer use efficiency through breeding, the graft-compatible genetic biodiversity existing in horticultural species offers the possibility to directly approach this objective in high-yielding elite varieties through improving nutrient capture and promoting ecosystem services such as insect pollination. We hypothesized that rootstocks affect pollinator foraging decisions through the nutritional status that impacts yield. Methods Fifteen genetically diverse experimental rootstocks were grafted to a scion tomato variety and cultivated under optimal and reduced (25% of optimal) P and NPK fertilization in the presence of managed bumblebee pollinators (Bombus terrestris). Results Up to 2-fold yield variability between rootstocks was associated with leaf nutrition and photosynthesis of the scion. Interestingly, fertilization regime and the rootstock genotype influenced the pollinator foraging decisions since bumblebees showed feeding preference for plants cultivated under low P, and for the most yielding and nutritious graft combinations under reduced but not under optimal fertilization. Bumblebees can sense plant nutritional status through source-sink relations, as supported by the consistent relationship between pollinator preferences and leaf carbon concentration. Conclusions This study opens new perspectives for using pollinators as “phenotypers” to select the most resilient plants under suboptimal conditions and/or genotypes that synergistically increase crop productivity by promoting the ecosystem service provided by the insects. 
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- 2023
12. CORRIGENDUM : Ectopic overexpression of the cell wall invertase gene CIN1 leads to dehydration avoidance in tomato
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Albacete, Alfonso, Cantero-Navarro, Elena, Großkinsky, Dominik K., Arias, Cintia L., Balibrea, María Encarnación, Bru, Roque, Fragner, Lena, Ghanem, Michel E., de la Cruz González, María, Hernández, Jose A., Martínez-Andújar, Cristina, van der Graaff, Eric, Weckwerth, Wolfram, Zellnig, Günther, Pérez-Alfocea, Francisco, and Roitsch, Thomas
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- 2015
13. Unravelling rootstock×scion interactions to improve food security
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Albacete, Alfonso, Martínez-Andújar, Cristina, Martínez-Pérez, Ascensión, Thompson, Andrew J., Dodd, Ian C., and Pérez-Alfocea, Francisco
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- 2015
14. Ectopic overexpression of the cell wall invertase gene CIN1 leads to dehydration avoidance in tomato
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Albacete, Alfonso, Cantero-Navarro, Elena, Großkinsky, Dominik K., Arias, Cintia L., Balibrea, María Encarnación, Bru, Roque, Fragner, Lena, Ghanem, Michel E., de la Cruz González, María, Hernández, Jose A., Martínez-Andújar, Cristina, van der Graaff, Eric, Weckwerth, Wolfram, Zellnig, Günther, Pérez-Alfocea, Francisco, and Roitsch, Thomas
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- 2015
15. Hormonal and metabolic regulation of source–sink relations under salinity and drought: From plant survival to crop yield stability
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Albacete, Alfonso A., Martínez-Andújar, Cristina, and Pérez-Alfocea, Francisco
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- 2014
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16. Hormonal and metabolic regulation of tomato fruit sink activity and yield under salinity
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Albacete, Alfonso, Cantero-Navarro, Elena, Balibrea, María E., Großkinsky, Dominik K., de la Cruz González, María, Martínez-Andújar, Cristina, Smigocki, Ann C., Roitsch, Thomas, and Pérez-Alfocea, Francisco
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- 2014
17. Multilevel interactions between native and ectopic isoprenoid pathways affect global metabolism in rice
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Consejo Superior de Investigaciones Científicas (España), Ministerio de Economía y Competitividad (España), European Commission, Pérez, Lucía, Alves, Rui, Pérez-Fons, Laura, Albacete, Alfonso, Farré, Gemma, Soto, E., Vilaprinyó, Ester, Martínez-Andújar, Cristina, Basallo, Oriol, Fraser, Paul D., Medina, Vicente, Zhu, Changfu, Capell, Teresa, Christou, Paul, Consejo Superior de Investigaciones Científicas (España), Ministerio de Economía y Competitividad (España), European Commission, Pérez, Lucía, Alves, Rui, Pérez-Fons, Laura, Albacete, Alfonso, Farré, Gemma, Soto, E., Vilaprinyó, Ester, Martínez-Andújar, Cristina, Basallo, Oriol, Fraser, Paul D., Medina, Vicente, Zhu, Changfu, Capell, Teresa, and Christou, Paul
- Abstract
Isoprenoids are natural products derived from isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). In plants, these precursors are synthesized via the cytosolic mevalonate (MVA) and plastidial methylerythritol phosphate (MEP) pathways. The regulation of these pathways must therefore be understood in detail to develop effective strategies for isoprenoid metabolic engineering. We hypothesized that the strict regulation of the native MVA pathway could be circumvented by expressing an ectopic plastidial MVA pathway that increases the accumulation of IPP and DMAPP in plastids. We therefore introduced genes encoding the plastid-targeted enzymes HMGS, tHMGR, MK, PMK and MVD and the nuclear-targeted transcription factor WR1 into rice and evaluated the impact of their endosperm-specific expression on (1) endogenous metabolism at the transcriptomic and metabolomic levels, (2) the synthesis of phytohormones, carbohydrates and fatty acids, and (3) the macroscopic phenotype including seed morphology. We found that the ectopic plastidial MVA pathway enhanced the expression of endogenous cytosolic MVA pathway genes while suppressing the native plastidial MEP pathway, increasing the production of certain sterols and tocopherols. Plants carrying the ectopic MVA pathway only survived if WR1 was also expressed to replenish the plastid acetyl-CoA pool. The transgenic plants produced higher levels of fatty acids, abscisic acid, gibberellins and lutein, reflecting crosstalk between phytohormones and secondary metabolism.
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- 2022
18. Induction of 9-cis-epoxycarotenoid dioxygenase in Arabidopsis thaliana seeds enhances seed dormancy
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Martínez-Andújar, Cristina, Ordiz, M. Isabel, Huang, Zhonglian, Nonogaki, Mariko, Beachy, Roger N., and Nonogaki, Hiroyuki
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- 2011
19. Root-synthesized cytokinins improve shoot growth and fruit yield in salinized tomato (Solanum Iycopersicum L.) plants
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Ghanem, Michel Edmond, Albacete, Alfonso, Smigocki, Ann C., Frébort, Ivo, Pospíšilová, Hana, Martínez-Andújar, Cristina, Acosta, Manuel, Sánchez-Bravo, José, Lutts, Stanley, Dodd, Ian C., and Pérez-Alfocea, Francisco
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- 2011
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20. Hormonal changes in relation to biomass partitioning and shoot growth impairment in salinized tomato (Solanum lycopersicum L.) plants
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Albacete, Alfonso, Ghanem, Michel Edmond, Martínez-Andújar, Cristina, Acosta, Manuel, Sánchez-Bravo, José, Martínez, Vicente, Lutts, Stanley, Dodd, Ian C., and Pérez-Alfocea, Francisco
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- 2008
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21. Hormonal changes during salinity-induced leaf senescence in tomato (Solanum lycopersicum L.)
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Ghanem, Michel Edmond, Albacete, Alfonso, Martínez-Andújar, Cristina, Acosta, Manuel, Romero-Aranda, Remedios, Dodd, Ian C., Lutts, Stanley, and Pérez-Alfocea, Francisco
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- 2008
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22. Hydrogen Peroxide Imbibition Following Cold Stratification Promotes Seed Germination Rate and Uniformity in Peach cv. GF305
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Barba-Espín, Gregorio, primary, Hernández, José A., additional, Martínez-Andújar, Cristina, additional, and Díaz-Vivancos, Pedro, additional
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- 2022
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23. Dynamic Hormone Gradients Regulate Wound-Induced de novo Organ Formation in Tomato Hypocotyl Explants
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Larriba, Eduardo, primary, Sánchez-García, Ana Belén, additional, Justamante, María Salud, additional, Martínez-Andújar, Cristina, additional, Albacete, Alfonso, additional, and Pérez-Pérez, José Manuel, additional
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- 2021
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24. Tissue-Specific Metabolic Reprogramming during Wound-Induced Organ Formation in Tomato Hypocotyl Explants
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Larriba, Eduardo, primary, Sánchez-García, Ana Belén, additional, Martínez-Andújar, Cristina, additional, Albacete, Alfonso, additional, and Pérez-Pérez, José Manuel, additional
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- 2021
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25. Overproduction of ABA in rootstocks alleviates salinity stress in tomato shoots
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European Commission, Ministerio de Economía y Competitividad (España), Agencia Estatal de Investigación (España), Biotechnology and Biological Sciences Research Council (UK), Martínez-Andújar, Cristina, Martínez Pérez, Ascensión, Albacete, Alfonso, Martínez-Melgarejo, Purificación A., Dodd, Ian C., Thompson, Andrew J., Mohareb, Fady, Estelles-López, Lucia, Kevei, Zoltan, Ferrández-Ayela, Almudena, Pérez-Pérez, José Manuel, Gifford, Miriam L., Pérez-Alfocea, Francisco, European Commission, Ministerio de Economía y Competitividad (España), Agencia Estatal de Investigación (España), Biotechnology and Biological Sciences Research Council (UK), Martínez-Andújar, Cristina, Martínez Pérez, Ascensión, Albacete, Alfonso, Martínez-Melgarejo, Purificación A., Dodd, Ian C., Thompson, Andrew J., Mohareb, Fady, Estelles-López, Lucia, Kevei, Zoltan, Ferrández-Ayela, Almudena, Pérez-Pérez, José Manuel, Gifford, Miriam L., and Pérez-Alfocea, Francisco
- Abstract
To determine whether root-supplied ABA alleviates saline stress, tomato (Solanum lycopersicum L. cv. Sugar Drop) was grafted onto two independent lines (NCED OE) overexpressing the SlNCED1 gene (9-cis-epoxycarotenoid dioxygenase) and wild type rootstocks. After 200 days of saline irrigation (EC = 3.5 dS m), plants with NCED OE rootstocks had 30% higher fruit yield, but decreased root biomass and lateral root development. Although NCED OE rootstocks upregulated ABA-signalling (AREB, ATHB12), ethylene-related (ACCs, ERFs), aquaporin (PIPs) and stress-related (TAS14, KIN, LEA) genes, downregulation of PYL ABA receptors and signalling components (WRKYs), ethylene synthesis (ACOs) and auxin-responsive factors occurred. Elevated SlNCED1 expression enhanced ABA levels in reproductive tissue while ABA catabolites accumulated in leaf and xylem sap suggesting homeostatic mechanisms. NCED OE also reduced xylem cytokinin transport to the shoot and stimulated foliar 2-isopentenyl adenine (iP) accumulation and phloem transport. Moreover, increased xylem GA levels in growing fruit trusses were associated with enhanced reproductive growth. Improved photosynthesis without changes in stomatal conductance was consistent with reduced stress sensitivity and hormone-mediated alteration of leaf growth and mesophyll structure. Combined with increases in leaf nutrients and flavonoids, systemic changes in hormone balance could explain enhanced vigour, reproductive growth and yield under saline stress.
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- 2021
26. Contrasting Rootstock-Mediated Growth and Yield Responses in Salinized Pepper Plants (Capsicum annuum L.) Are Associated with Changes in the Hormonal Balance
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Gálvez, Amparo, primary, Albacete, Alfonso, additional, Martínez-Andújar, Cristina, additional, del Amor, Francisco M., additional, and López-Marín, Josefa, additional
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- 2021
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27. Genetic Analysis of Root-to-Shoot Signaling and Rootstock-Mediated Tolerance to Water Deficit in Tomato
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Ministerio de Ciencia, Innovación y Universidades (España), Ministerio de Economía y Competitividad (España), European Commission, Asins, M. J., Albacete, Alfonso, Martínez-Andújar, Cristina, Celiktopuz, Eser, Solmaz, İlknur, Sarı, Nebahat, Pérez-Alfocea, Francisco, Dodd, Ian C., Carbonell, Emilio A., Topcu, Sevilay, Ministerio de Ciencia, Innovación y Universidades (España), Ministerio de Economía y Competitividad (España), European Commission, Asins, M. J., Albacete, Alfonso, Martínez-Andújar, Cristina, Celiktopuz, Eser, Solmaz, İlknur, Sarı, Nebahat, Pérez-Alfocea, Francisco, Dodd, Ian C., Carbonell, Emilio A., and Topcu, Sevilay
- Abstract
Developing drought-tolerant crops is an important strategy to mitigate climate change impacts. Modulating root system function provides opportunities to improve crop yield under biotic and abiotic stresses. With this aim, a commercial hybrid tomato variety was grafted on a genotyped population of 123 recombinant inbred lines (RILs) derived from Solanum pimpinellifolium, and compared with self- and non-grafted controls, under contrasting watering treatments (100% vs. 70% of crop evapotranspiration). Drought tolerance was genetically analyzed for vegetative and flowering traits, and root xylem sap phytohormone and nutrient composition. Under water deficit, around 25% of RILs conferred larger total shoot dry weight than controls. Reproductive and vegetative traits under water deficit were highly and positively correlated to the shoot water content. This association was genetically supported by linkage of quantitative trait loci (QTL) controlling these traits within four genomic regions. From a total of 83 significant QTLs, most were irrigation-regime specific. The gene contents of 8 out of 12 genomic regions containing 46 QTLs were found significantly enriched at certain GO terms and some candidate genes from diverse gene families were identified. Thus, grafting commercial varieties onto selected rootstocks derived from S. pimpinellifolium provides a viable strategy to enhance drought tolerance in tomato.
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- 2020
28. Genetic Analysis of Root-to-Shoot Signaling and Rootstock-Mediated Tolerance to Water Deficit in Tomato
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Asins, Maria J., primary, Albacete, Alfonso, additional, Martínez-Andújar, Cristina, additional, Celiktopuz, Eser, additional, Solmaz, İlknur, additional, Sarı, Nebahat, additional, Pérez-Alfocea, Francisco, additional, Dodd, Ian C., additional, Carbonell, Emilio A., additional, and Topcu, Sevilay, additional
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- 2020
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29. Mechanisms of hormonal regulation of endosperm cap-specific gene expression in tomato seeds
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Martínez-Andújar, Cristina, Pluskota, Wioletta E., Bassel, George W., Asahina, Masashi, Pupel, Piotr, Nguyen, Theresa T., Takeda-Kamiya, Noriko, Toubiana, David, Bai, Bing, Górecki, Ryszard J., Fait, Aaron, Yamaguchi, Shinjiro, and Nonogaki, Hiroyuki
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- 2012
- Full Text
- View/download PDF
30. Seed Traits and Genes Important for Translational Biology—Highlights from Recent Discoveries
- Author
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Martínez-Andújar, Cristina, Martin, Ruth C., and Nonogaki, Hiroyuki
- Published
- 2012
- Full Text
- View/download PDF
31. Root-synthesized cytokinins improve shoot growth and fruit yield in salinized tomato (Solanum lycopersicum L.) plants
- Author
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Ghanem, Michel Edmond, Albacete, Alfonso, Smigocki, Ann C., Frébort, Ivo, Pospíšilová, Hana, Martínez-Andújar, Cristina, Acosta, Manuel, Sánchez-Bravo, José, Lutts, Stanley, Dodd, Ian C., and Pérez-Alfocea, Francisco
- Published
- 2011
- Full Text
- View/download PDF
32. Respuesta a la sobreproducción de ABA en salinidad
- Author
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Martínez Pérez, Ascensión, Ferrández Ayela, A., Sánchez García, A.B., Albacete, A., Kevei, Z., Estellés, L., Mohareb, F., Thompson, A.J., Gifford, M., Pérez Pérez, J.M., Pérez Alfocea, Francisco, Martínez Andújar, Cristina, and Universidad Politécnica de Cartagena
- Subjects
Phytohormones ,WiA ,Phytohormone ,Microarrays ,Fitohormona ,Expresión de genes ,Fitohormonas ,Transgénicos ,Gene expression ,Tecnología de los Alimentos ,Transgenics - Abstract
[SPA] Con el fin de comprender la influencia de la fitohormona ácido abscísico (ABA) en la adaptación al riego salino, dos líneas transgénicas independientes de tomate (Solanum lycopersicum L.), sp12 y sp5, que sobreexpresan constitutivamente el gen NCED1 (codifica para la enzima que cataliza un paso limitante en la biosíntesis de ABA) y la variedad silvestre Ailsa Craig, se han estudiado en experimentos o bien i) como planta entera o ii) como portainjerto bajo condiciones control y de estrés salino. Aunque la expresión constitutiva de NCED disminuye el crecimiento bajo condiciones control, minimiza los efectos producidos por la sal (planta completa) y mejora significativamente el crecimiento cuando se usa como portainjerto. El análisis de la savia xilemática de raíz mostró que los fenotipos resultantes bajo las diferentes condiciones de cultivo eran difíciles de explicar en términos de sobreproducción de ABA. Para intentar explicar estos resultados se llevó a cabo un análisis de expresión de un conjunto de genes relacionados con hormonas y estrés mediante PCR cuantitativa, así como un estudio transcriptómico mediante microarrays en la raíz. Los resultados sugieren que la sobreexpresión de NCED parece alterar diversas rutas de señalización, derivando en una respuesta adaptativa al estrés que podría ayudar a explicar los fenotipos observados. [ENG] With the aim of better understanding the influence of the plan hormone abscisic acid (ABA) in adaptation to saline irrigation, two independent transgenic tomato (Solanum lycopersicum L.) lines, sp12 and sp5, overexpressing constitutively NCED1 (the enzyme that catalyzes a key rate-limiting step in ABA biosynthesis) and the wild type Ailsa Craig, have been studied in experiments either i) as whole plants or ii) as rootstocks under control and salinity conditions. While NCED overexpression penalizes growth under control conditions, it minimized the effect of salinity (whole plants) or significantly improved plant growth and yield when used as rootstocks. The analysis of the root xylem sap revealed that the phenotypes resulting under the different conditions were difficult to explain in terms of ABA overproduction. With the aim of explaining these results, the expression of a set of hormone and stress associated genes (analysed by real time PCR) as well as a transcriptomic analysis (by using one-color microarray) were performed in roots. The results suggest that NCED overexpression seems to alter several signalling pathways leading to stress adaptive responses that could help to explain the observed phenotypes. The authors thank Andrew J. Thompson from Cranfield University, the NCED seeds set. This work was supported by CICYT-FEDER (project AGL2011-27996) and European Union’s Seventh Framework Programme for research, technological development and demonstration under grant agreement no 289365(ROOTOPOWER project).
- Published
- 2018
33. Señalización de ABA en la raíz de tomate bajo estrés salino
- Author
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Martínez Pérez, A., Ferrández Ayela, A., Sánchez García, A.B., Albacete Moreno, Alfonso, Kevei, Z., Estellés, L., Mohareb, F., Thompson, A.J., Gifford, M., Pérez Pérez, J.M., Pérez Alfocea, Francisco, Martínez Andújar, Cristina, and Universidad Politécnica de Cartagena
- Subjects
Phytohormones ,WiA ,Microarrays ,Expresión de genes ,Fitohormonas ,Transgénicos ,Gene expression ,Tecnología de los Alimentos ,Transgenics - Abstract
[SPA] Con el fin de comprender la influencia de la fitohormona ácido abscísico (ABA) en la adaptación al riego salino, dos líneas transgénicas independientes de tomate (Solanum lycopersicum L.), sp12 y sp5, que sobreexpresan constitutivamente el gen NCED1 (codifica para la enzima que cataliza un paso limitante en la biosíntesis de ABA) y la variedad silvestre Ailsa Craig, se han estudiado en experimentos o bien i) como planta entera o ii) como portainjerto bajo condiciones control y de estrés salino. Aunque la expresión constitutiva de NCED disminuye el crecimiento bajo condiciones control, minimiza los efectos producidos por la sal (planta completa) y mejora significativamente el crecimiento cuando se usa como portainjerto. El análisis de la savia xilemática de raíz mostró que los fenotipos resultantes bajo las diferentes condiciones de cultivo eran difíciles de explicar en términos de sobreproducción de ABA. Para intentar explicar estos resultados se llevó a cabo un análisis de expresión de un conjunto de genes relacionados con hormonas y estrés mediante PCR cuantitativa, así como un estudio transcriptómico mediante microarrays en la raíz. Los resultados sugieren que la sobreexpresión de NCED parece alterar diversas rutas de señalización, derivando en una respuesta adaptativa al estrés que podría ayudar a explicar los fenotipos observados. [ENG] With the aim of better understanding the influence of the plan hormone abscisic acid (ABA) in adaptation to saline irrigation, two independent transgenic tomato (Solanum lycopersicum L.) lines, sp12 and sp5, overexpressing constitutively NCED1 (the enzyme that catalyzes a key rate-limiting step in ABA biosynthesis) and the wild type Ailsa Craig, have been studied in experiments either i) as whole plants or ii) as rootstocks under control and salinity conditions. While NCED overexpression penalizes growth under control conditions, it minimized the effect of salinity (whole plants) or significantly improved plant growth and yield when used as rootstocks. The analysis of the root xylem sap revealed that the phenotypes resulting under the different conditions were difficult to explain in terms of ABA overproduction. With the aim of explaining these results, the expression of a set of hormone and stress associated genes (analysed by real time PCR) as well as a transcriptomic analysis (by using one-color microarray) were performed in roots. The results suggest that NCED overexpression seems to alter several signalling pathways leading to stress adaptive responses that could help to explain the observed phenotypes. The authors thank Andrew J. Thompson from Cranfield University, the NCED seeds set. This work was supported by CICYT-FEDER (project AGL2011-27996) and European Union’s Seventh Framework Programme for research, technological development and demonstration under grant agreement no 289365(ROOTOPOWER project).. -2010-5 European Union).
- Published
- 2017
34. Hormonal and Nutritional Features in Contrasting Rootstock-mediated Tomato Growth under Low-phosphorus Nutrition
- Author
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European Commission, Ministerio de Economía y Competitividad (España), Martínez-Andújar, Cristina, Ruiz-Lozano, Juan Manuel, Dodd, Ian C., Albacete, Alfonso, Pérez-Alfocea, Francisco, European Commission, Ministerio de Economía y Competitividad (España), Martínez-Andújar, Cristina, Ruiz-Lozano, Juan Manuel, Dodd, Ian C., Albacete, Alfonso, and Pérez-Alfocea, Francisco
- Abstract
Grafting provides a tool aimed to increase low-P stress tolerance of crops, however, little is known about the mechanism (s) by which rootstocks can confer resistance to P deprivation. In this study, 4 contrasting groups of rootstocks from different genetic backgrounds (Solanum lycopersicum var. cerasiforme and introgression and recombinant inbred lines derived from the wild relatives S. pennellii and S. pimpinellifolium) were grafted to a commercial F1 hybrid scion and cultivated under control (1 mM, c) and P deficient (0.1 mM, p) conditions for 30 days, to analyze rootstocks-mediated traits that impart low (L, low shoot dry weight, SDW) or high (H, high SDW) vigor. Xylem sap ionic and hormonal anlyses leaf nutritional status suggested that some physiological traits can explain rootstocks impacts on shoot growth. Although xylem P concentration increased with root biomass under both growing conditions, shoot biomass under low-P was explained by neither changes in root growth nor P transport and assimilation. Indeed, decreased root P export only explained the sensitivity of the HcLp rootstocks, while leaf P status was similarly affected in all graft combinations. Interestingly, most of the nutrients analyzed in the xylem sap correlated with root biomass under standard fertilization but only Ca was consistently related to shoot biomass under both control and low-P, suggesting an important role for this nutrient in rootstock-mediated vigor. Moreover, foliar Ca, S, and Mn concentrations were (i) specifically correlated with shoot growth under low-P and (ii) positively and negatively associated to the root-to-shoot transport of the cytokinin trans-zeatin (t-Z) and the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC), respectively. Indeed, those hormones seem to play an antagonistic positive (t-Z) and negative (ACC) role in the rootstock-mediated regulation of shoot growth in response to P nutrition. The use of Hp-type rootstocks seems to enhance P use effic
- Published
- 2017
35. Interaction between ABA and cytokinins in salinized tomato plants
- Author
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Martínez Pérez, A., Bahar Oztekin, G., Albacete Moreno, Alfonso, Thompson, A.J., Sánchez Iglesias, María del Puerto, Pérez Alfocea, Francisco, Martínez Andújar, Cristina, and Universidad Politécnica de Cartagena
- Subjects
Phytohormones ,Hydroponic ,Grafting ,fungi ,food and beverages ,Tecnología de los Alimentos ,Transgenics - Abstract
Abscisic acid (ABA) and cytokinins (CKs) are phytohormones that mediate plant responses to abiotic stress and are mainly considered to be produced in roots. These two plant hormones have long been considered antagonists, but the role of this antagonism in mediating abiotic stress resistance remains unknown. In this study we have used reciprocal grafting of transgenic lines with altered hormone synthesis (ABA or CK synthesis) with the aim of assessing the importance of ABA and CK interaction in mediating plant salinity responses in tomato. Preliminary results have shown a negative effect in ABA and CK on tomato plant biomass under salinity. Results have also indicated that jasmonic acid (JA) might be involved in the negative impact on tomato growth caused by ABA and CK interaction under salt conditions. The authors thank Andrew J. Thompson, from Cranfield Univertity (UK), and Ann C. Smigocki from Molecular Plant Pathology Laboratory (EEUU) the NCED and IPT seeds sets respectively. This work was supported by CICYT AGL2011‐27996 and ROOTOPOWER (REF: 289365. FP7‐KBBE‐2011‐5 European Union).
- Published
- 2016
36. Hormonal and Nutritional Features in Contrasting Rootstock-mediated Tomato Growth under Low-phosphorus Nutrition
- Author
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Martínez-Andújar, Cristina, primary, Ruiz-Lozano, Juan M., additional, Dodd, Ian C., additional, Albacete, Alfonso, additional, and Pérez-Alfocea, Francisco, additional
- Published
- 2017
- Full Text
- View/download PDF
37. Root-to-Shoot Hormonal Communication in Contrasting Rootstocks Suggests an Important Role for the Ethylene Precursor Aminocyclopropane-1-carboxylic Acid in Mediating Plant Growth under Low-Potassium Nutrition in Tomato
- Author
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Martínez-Andújar, Cristina, primary, Albacete, Alfonso, additional, Martínez-Pérez, Ascensión, additional, Pérez-Pérez, José Manuel, additional, Asins, María José, additional, and Pérez-Alfocea, Francisco, additional
- Published
- 2016
- Full Text
- View/download PDF
38. Exploring the use of recombinant inbred lines in combination with beneficial microbial inoculants (AM fungus and PGPR) to improve drought stress tolerance in tomato
- Author
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UCL - SST/ELI/ELIM - Applied Microbiology, Calvo-Polanco, Mónica, Sánchez-Romera, Beatriz, Aroca, Ricardo, Asins, María José, Declerck, Stephan, Dodd, Ian C., Martínez-Andújar, Cristina, Albacete, Alfonso, Ruiz-Lozano, Juan Manuel, UCL - SST/ELI/ELIM - Applied Microbiology, Calvo-Polanco, Mónica, Sánchez-Romera, Beatriz, Aroca, Ricardo, Asins, María José, Declerck, Stephan, Dodd, Ian C., Martínez-Andújar, Cristina, Albacete, Alfonso, and Ruiz-Lozano, Juan Manuel
- Abstract
At a world scale, tomato is an important horticultural crop, but its productivity is highly reduced by drought stress. Combining the application of beneficial microbial inoculants with breeding and grafting techniques may be key to cope with reduced tomato yield under drought. This study aimed to investigate the growth responses and physiological mechanisms involved in the performance under drought stress of four tomato recombinant inbred lines (RIL) after inoculation with the arbuscular mycorrhizal (AM) fungus Rhizophagus irregularis and the plant growth promoting rhizobacteria (PGPR) Variovorax paradoxus 5C-2. Results showed a variation in the efficiency of the different tomato RILs under drought stress and a differential effect of the microbial inoculants, depending on the RIL involved. The inoculants affected plant parameters such as net photosynthetic capacity, oxidative damage to lipids, osmolyte accumulation, root hydraulic conductivity or aquaporin abundance and phosphorylation status. RIL66 was the one obtaining maximum benefit from the microbial inoculants under drought stress conditions, due likely to improved CO2-fixation capacity and root hydraulic conductivity. We propose that RIL66 could be selected as a good plant material to be used as rootstock to improve tomato growth and productivity under water limiting conditions. Since RIL66 is highly responsive to microbial inoculants, this grafting strategy should be combined with inoculation of R. irregularis and V. paradoxus in order to improve plant yield under conditions of drought stress.
- Published
- 2016
39. Exploring the use of recombinant inbred lines in combination with beneficial microbial inoculants (AM fungus and PGPR) to improve drought stress tolerance in tomato
- Author
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European Commission, Calvo-Polanco, Mónica, Sánchez-Romera, Beatriz, Aroca, Ricardo, Asins, María J., Declerck, Stéphane, Dodd, Ian C., Martínez-Andújar, Cristina, Albacete, Alfonso, Ruiz-Lozano, Juan Manuel, European Commission, Calvo-Polanco, Mónica, Sánchez-Romera, Beatriz, Aroca, Ricardo, Asins, María J., Declerck, Stéphane, Dodd, Ian C., Martínez-Andújar, Cristina, Albacete, Alfonso, and Ruiz-Lozano, Juan Manuel
- Abstract
At a world scale, tomato is an important horticultural crop, but its productivity is highly reduced by drought stress. Combining the application of beneficial microbial inoculants with breeding and grafting techniques may be key to cope with reduced tomato yield under drought. This study aimed to investigate the growth responses and physiological mechanisms involved in the performance under drought stress of four tomato recombinant inbred lines (RIL) after inoculation with the arbuscular mycorrhizal (AM) fungus Rhizophagus irregularis and the plant growth promoting rhizobacteria (PGPR) Variovorax paradoxus 5C-2. Results showed a variation in the efficiency of the different tomato RILs under drought stress and a differential effect of the microbial inoculants, depending on the RIL involved. The inoculants affected plant parameters such as net photosynthetic capacity, oxidative damage to lipids, osmolyte accumulation, root hydraulic conductivity or aquaporin abundance and phosphorylation status. RIL66 was the one obtaining maximum benefit from the microbial inoculants under drought stress conditions, due likely to improved CO2-fixation capacity and root hydraulic conductivity. We propose that RIL66 could be selected as a good plant material to be used as rootstock to improve tomato growth and productivity under water limiting conditions. Since RIL66 is highly responsive to microbial inoculants, this grafting strategy should be combined with inoculation of R. irregularis and V. paradoxus in order to improve plant yield under conditions of drought stress.[EN]
- Published
- 2016
40. Root-to-Shoot Hormonal Communication in Contrasting Rootstocks Suggests an Important Role for the Ethylene Precursor Aminocyclopropane-1-carboxylic Acid in Mediating Plant Growth under Low-Potassium Nutrition in Tomato
- Author
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Ministerio de Economía y Competitividad (España), European Commission, Martínez-Andújar, Cristina, Albacete, Alfonso, Martínez Pérez, Ascensión, Pérez-Pérez, José Manuel, Asins, M. J., Pérez-Alfocea, Francisco, Ministerio de Economía y Competitividad (España), European Commission, Martínez-Andújar, Cristina, Albacete, Alfonso, Martínez Pérez, Ascensión, Pérez-Pérez, José Manuel, Asins, M. J., and Pérez-Alfocea, Francisco
- Abstract
Selection and breeding of rootstocks that can tolerate low K supply may increase crop productivity in low fertility soils and reduce fertilizer application. However, the underlying physiological traits are still largely unknown. In this study, 16 contrasting recombinant inbred lines (RILs) derived from a cross between domestic and wild tomato species (Solanum lycopersicum × Solanum pimpinellifolium) have been used to analyse traits related to the rootstock-mediated induction of low (L, low shoot fresh weight) or high (H, high shoot fresh weight) vigor to a commercial F1 hybrid grown under control (6 mM, c) and low-K (1 mM, k). Based on hormonal and ionomic composition in the root xylem sap and the leaf nutritional status after long-term (7 weeks) exposure low-K supply, a model can be proposed to explain the rootstocks effects on shoot performance with the ethylene precursor aminocyclopropane-1-carboxylic acid (ACC) playing a pivotal negative role. The concentration of this hormone was higher in the low-vigor Lc and Lk rootstocks under both conditions, increased in the sensitive HcLk plants under low-K while it was reduced in the high-vigor Hk ones. Low ACC levels would promote the transport of K vs. Na in the vigorous Hk grafted plants. Along with K, Ca, and S, micronutrient uptake and transport were also activated in the tolerant Hk combinations under low-K. Additionally, an interconversion of trans-zeatin into trans-zeatin riboside would contribute to decrease ACC in the tolerant LcHk plants. The high vigor induced by the Hk plants can also be explained by an interaction of ACC with other hormones (cytokinins and salicylic, abscisic and jasmonic acids). Therefore, Hk rootstocks convert an elite tomato F1 cultivar into a (micro) nutrient-efficient phenotype, improving growth under reduced K fertilization.
- Published
- 2016
41. Efectos de la sobreproducción de ácido abscísico y citoquininasen la respuesta fisiológica y agronómica de tomate bajo estrés salino en invernadero
- Author
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Martínez Pérez, Ascensión, Albacete Moreno, Alfonso, Sánchez Iglesias, María del Puerto, Pérez Alfocea, Francisco, Martínez Andújar, Cristina, Técnicas Avanzadas en Investigación y Desarrollo Agrario y Alimentario (TAIDA), Escuela Técnica Superior de Ingeniería (ETSIA), Instituto de Biotecnología Vegetal (IBV), Universidad Politécnica de Cartagena, and Campus Mare Nostrum
- Subjects
Transgénico ,Injertos ,Fitohormonas ,Salinidad - Abstract
La salinidad limita la productividad de los cultivos y modifica el balance hormonal vegetal. El ácido abscísico (ABA) y las citoquininas (CKs) son fitohormonas que median la respuesta a estreses abióticos y se considera que son producidas principalmente en la raíz. Para evaluar el papel de estas dos hormonas en el crecimiento y productividad de los cultivos bajo salinidad, se realizaron injertos cuyo portainjerto eran unas variedades transgénicas de tomate que sobreproducían ácido abscísico (ABA) (líneas NCED) y citoquininas (líneas IPT), utilizando una variedad comercial de tomate cherry como injerto. Las plantas de tomate injertadas fueron cultivadas en un invernadero comercial durante 6 meses en condiciones de salinidad moderada. Las plantas cuyo portainjerto era una variedad transgénica eran más vigorosas y presentaban mayor producción que las que tenían como portainjerto una planta wild type o la propia parte aérea injertada. Los análisis hormonales no explican completamente estos datos, por lo que otros tipos de análisis (genéticos, enzimáticos) son necesarios para explicar completamente estos datos.
- Published
- 2014
42. Efectos de la sobreproducción de ácido abscísico y citoquininas en la respuesta fisiológica y agronómica de tomate bajo estrés salino en invernadero
- Author
-
Martínez Pérez, Ascensión, Albacete Moreno, Alfonso, Sánchez Iglesias, María del Puerto, Pérez Alfocea, Francisco, Martínez Andújar, Cristina, and Universidad Politécnica de Cartagena
- Subjects
WiA ,Injertos ,Fitohormonas ,Transgénicos ,Salinidad ,Tecnología de los Alimentos - Abstract
La salinidad limita la productividad de los cultivos y modifica el balance hormonal vegetal. El ácido abscísico (ABA) y las citoquininas (CKs) son fitohormonas que median la respuesta a estreses abióticos y se considera que son producidas principalmente en la raíz. Para evaluar el papel de estas dos hormonas en el crecimiento y productividad de los cultivos bajo salinidad, se realizaron injertos cuyo portainjerto eran unas variedades transgénicas de tomate que sobreproducían ácido abscísico (ABA) (líneas NCED) y citoquininas (líneas IPT), utilizando una variedad comercial de tomate cherry como injerto. Las plantas de tomate injertadas fueron cultivadas en un invernadero comercial durante 6 meses en condiciones de salinidad moderada. Las plantas cuyo portainjerto era una variedad transgénica eran más vigorosas y presentaban mayor producción que las que tenían como portainjerto una planta wild type o la propia parte aérea injertada. Los análisis hormonales no explican completamente estos datos, por lo que otros tipos de análisis (genéticos, enzimáticos) son necesarios para explicar completamente estos datos. Los autores de la presente comunicación desean agradecer a Andrew J. Thompson de la Universidad de Cranfield (Reino Unido) y a Ann C. Smigocki del Molecular Plant Pathology Laboratory (EEUU) por los sets de semillas NCED e IPT respectivamente. Este trabajo ha sido financiado por CICYT AGL2011‐27996 y ROOTOPOWER (REF: 289365. FP7‐KBBE‐2011‐5 European Union)
- Published
- 2014
43. Hormonal and metabolic regulation of tomato fruit sink activity and yield under salinity
- Author
-
Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular, Albacete, Alfonso, Cantero Navarro, Elena, Balibrea, Mª Encarnación, Großkinsky, Dominik K., González García, María de la Cruz, Martínez Andújar, Cristina, Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular, Albacete, Alfonso, Cantero Navarro, Elena, Balibrea, Mª Encarnación, Großkinsky, Dominik K., González García, María de la Cruz, and Martínez Andújar, Cristina
- Abstract
Salinization of water and soil has a negative impact on tomato (Solanum lycopersicum L.) productivity by reducing growth of sink organs and by inducing senescence in source leaves. It has been hypothesized that yield stability implies the maintenance or increase of sink activity in the reproductive structures, thus contributing to the transport of assimilates from the source leaves through changes in sucrolytic enzymes and their regulation by phytohormones. In this study, classical and functional physiological approaches have been integrated to study the influence of metabolic and hormonal factors on tomato fruit sink activity, growth, and yield: (i) exogenous hormones were applied to plants, and (ii) transgenic plants overexpressing the cell wall invertase (cwInv) gene CIN1 in the fruits and de novo cytokinin (CK) biosynthesis gene IPT in the roots were constructed. Although salinity reduces fruit growth, sink activity, and trans-zeatin (tZ) concentrations, it increases the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) during the actively growing period (25 days after anthesis). Indeed, exogenous application of the CK analogue kinetin to salinized actively growing fruits recovered sucrolytic activities (mainly cwInv and sucrose synthase), sink strength, and fruit weight, whereas the ethylene-releasing compound ethephon had a negative effect in equivalent non-stressed fruits. Fruit yield was increased by both the constitutive expression of CIN1 in the fruits (up to 4-fold) or IPT in the root (up to 30%), owing to an increase in the fruit number (lower flower abortion) and in fruit weight. This is possibly related to a recovery of sink activity in reproductive tissues due to both (i) increase in sucrolytic activities (cwInv, sucrose synthase, and vacuolar and cytoplasmic invertases) and tZ concentration, and (ii) a decrease in the ACC levels and the activity of the invertase inhibitor. This study provides new functional evidences about the role of me
- Published
- 2014
44. Ectopic overexpression of the cell wall invertase gene CIN1 leads to dehydration avoidance in tomato
- Author
-
Universidad de Alicante. Departamento de Agroquímica y Bioquímica, Albacete, Alfonso, Cantero-Navarro, Elena, Großkinsky, Dominik K., Arias, Cintia L., Balibrea, María Encarnación, Bru-Martinez, Roque, Fragner, Lena, Ghanem, Michel E., González, María de la Cruz, Hernández, Jose A., Martínez-Andújar, Cristina, Graaff, Eric van der, Weckwerth, Wolfram, Zellnig, Günther, Pérez-Alfocea, Francisco, Roitsch, Thomas, Universidad de Alicante. Departamento de Agroquímica y Bioquímica, Albacete, Alfonso, Cantero-Navarro, Elena, Großkinsky, Dominik K., Arias, Cintia L., Balibrea, María Encarnación, Bru-Martinez, Roque, Fragner, Lena, Ghanem, Michel E., González, María de la Cruz, Hernández, Jose A., Martínez-Andújar, Cristina, Graaff, Eric van der, Weckwerth, Wolfram, Zellnig, Günther, Pérez-Alfocea, Francisco, and Roitsch, Thomas
- Abstract
Drought stress conditions modify source–sink relations, thereby influencing plant growth, adaptive responses, and consequently crop yield. Invertases are key metabolic enzymes regulating sink activity through the hydrolytic cleavage of sucrose into hexose monomers, thus playing a crucial role in plant growth and development. However, the physiological role of invertases during adaptation to abiotic stress conditions is not yet fully understood. Here it is shown that plant adaptation to drought stress can be markedly improved in tomato (Solanum lycopersicum L.) by overexpression of the cell wall invertase (cwInv) gene CIN1 from Chenopodium rubrum. CIN1 overexpression limited stomatal conductance under normal watering regimes, leading to reduced water consumption during the drought period, while photosynthetic activity was maintained. This caused a strong increase in water use efficiency (up to 50%), markedly improving water stress adaptation through an efficient physiological strategy of dehydration avoidance. Drought stress strongly reduced cwInv activity and induced its proteinaceous inhibitor in the leaves of the wild-type plants. However, the CIN1-overexpressing plants registered 3- to 6-fold higher cwInv activity in all analysed conditions. Surprisingly, the enhanced invertase activity did not result in increased hexose concentrations due to the activation of the metabolic carbohydrate fluxes, as reflected by the maintenance of the activity of key enzymes of primary metabolism and increased levels of sugar-phosphate intermediates under water deprivation. The induced sink metabolism in the leaves explained the maintenance of photosynthetic activity, delayed senescence, and increased source activity under drought stress. Moreover, CIN1 plants also presented a better control of production of reactive oxygen species and sustained membrane protection. Those metabolic changes conferred by CIN1 overexpression were accompanied by increases in the concentrations of the s
- Published
- 2014
45. Ectopic overexpression of the cell wall invertase gene CIN1 leads to dehydration avoidance in tomato
- Author
-
Albacete, Alfonso, primary, Cantero-Navarro, Elena, additional, Großkinsky, Dominik K., additional, Arias, Cintia L., additional, Balibrea, María Encarnación, additional, Bru, Roque, additional, Fragner, Lena, additional, Ghanem, Michel E., additional, González, María de la Cruz, additional, Hernández, Jose A., additional, Martínez-Andújar, Cristina, additional, van der Graaff, Eric, additional, Weckwerth, Wolfram, additional, Zellnig, Günther, additional, Pérez-Alfocea, Francisco, additional, and Roitsch, Thomas, additional
- Published
- 2014
- Full Text
- View/download PDF
46. Análisis funcional de una asparragina sintetasa dependiente de amonio en tomate (solanum lycopersicon L.) cultivado en condiciones de estrés salino y diferente nutrición nitrogenada / Cristina Martínez Andújar; director Francisco Pérez Alfocea
- Author
-
Martínez Andújar, Cristina
- Subjects
631.541(043) [635.64] ,Tomate - Abstract
Tesis-Universidad de Murcia., Consulte la tesis en: BCA. GENERAL. ARCHIVO UNIVERSITARIO. T.M. 3030.
- Published
- 2006
47. Root-synthesized cytokinins improve shoot growth and fruit yield in salinized tomato (Solanum lycopersicum L.) plants.
- Author
-
UCL - SST/ELI/ELIA - Agronomy, Ghanem, Michel Edmond, Albacete, Alfonso, Smigocki, Ann C, Frébort, Ivo, Pospísilová, Hana, Martínez-Andújar, Cristina, Acosta, Manuel, Sánchez-Bravo, José, Lutts, Stanley, Dodd, Ian C., Pérez-Alfocea, Francisco, UCL - SST/ELI/ELIA - Agronomy, Ghanem, Michel Edmond, Albacete, Alfonso, Smigocki, Ann C, Frébort, Ivo, Pospísilová, Hana, Martínez-Andújar, Cristina, Acosta, Manuel, Sánchez-Bravo, José, Lutts, Stanley, Dodd, Ian C., and Pérez-Alfocea, Francisco
- Abstract
Salinity limits crop productivity, in part by decreasing shoot concentrations of the growth-promoting and senescence-delaying hormones cytokinins. Since constitutive cytokinin overproduction may have pleiotropic effects on plant development, two approaches assessed whether specific root-localized transgenic IPT (a key enzyme for cytokinin biosynthesis) gene expression could substantially improve tomato plant growth and yield under salinity: transient root IPT induction (HSP70::IPT) and grafting wild-type (WT) shoots onto a constitutive IPT-expressing rootstock (WT/35S::IPT). Transient root IPT induction increased root, xylem sap, and leaf bioactive cytokinin concentrations 2- to 3-fold without shoot IPT gene expression. Although IPT induction reduced root biomass (by 15%) in control (non-salinized) plants, in salinized plants (100?mM NaCl for 22?d), increased cytokinin concentrations delayed stomatal closure and leaf senescence and almost doubled shoot growth (compared with WT plants), with concomitant increases in the essential nutrient K(+) (20%) and decreases in the toxic ion Na(+) (by 30%) and abscisic acid (by 20-40%) concentrations in transpiring mature leaves. Similarly, WT/35S::IPT plants (scion/rootstock) grown with 75?mM NaCl for 90?d had higher fruit trans-zeatin concentrations (1.5- to 2-fold) and yielded 30% more than WT/non-transformed plants. Enhancing root cytokinin synthesis modified both shoot hormonal and ionic status, thus ameliorating salinity-induced decreases in growth and yield.
- Published
- 2011
48. Exploiting root-to-shoot hormonal communication to improve salt tolerance in tomato
- Author
-
UCL - SC/BIOL - Département de biologie, -, Ghanem, Michel Edmond, Albacete, Alfonso, Martínez-Andújar, Cristina, Dodd , Ian C., Pérez-Alfocea, Francisco, INTERDROUGHT III, UCL - SC/BIOL - Département de biologie, -, Ghanem, Michel Edmond, Albacete, Alfonso, Martínez-Andújar, Cristina, Dodd , Ian C., Pérez-Alfocea, Francisco, and INTERDROUGHT III
- Published
- 2009
49. Rootstock-mediated changes in xylem ionic and hormonal status are correlated with delayed leaf senescence and increased leaf area and crop productivity in salinised tomato.
- Author
-
Albacete, Alfonso, Martínez-Andújar, Cristina, Ghanem, Michel Edmond, Acosta, Manuel, Sanchez-Bravo, Jose, Asins, Maria J., Cuartero, Jesus, Lutts, Stanley, Dodd, Ian C., Pérez-Alfocea, Francisco, Albacete, Alfonso, Martínez-Andújar, Cristina, Ghanem, Michel Edmond, Acosta, Manuel, Sanchez-Bravo, Jose, Asins, Maria J., Cuartero, Jesus, Lutts, Stanley, Dodd, Ian C., and Pérez-Alfocea, Francisco
- Abstract
Tomato crop productivity under salinity can be improved by grafting cultivars onto salt-tolerant wild relatives, thus mediating the supply of root-derived ionic and hormonal factors that regulate leaf area and senescence. A tomato cultivar was grafted onto rootstocks from a population of recombinant inbred lines (RILs) derived from a Solanum lycopersicum × Solanum cheesmaniae cross and cultivated under moderate salinity (75 mm NaCl). Concentrations of Na+, K+ and several phytohormones [abscisic acid (ABA); the cytokinins (CKs) zeatin, Z; zeatin riboside, ZR; and the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC)] were analysed in leaf xylem sap in graft combinations of contrasting vigour. Scion leaf area correlated with photosystem II (PSII) efficiency (Fv/Fm) and determined fruit productivity. Xylem K+ (but not Na+), K+/Na+, the active CK Z, the ratio with its storage form Z/ZR and especially the ratio between CKs and ACC (Z/ACC and Z + ZR/ACC) were positively loaded into the first principal component (PC) determining both leaf growth and PSII efficiency. In contrast, the ratio ACC/ABA was negatively correlated with leaf biomass. Although the underlying physiological mechanisms by which rootstocks mediate leaf area or chlorophyll fluorescence (and thus influence tomato salt tolerance) seem complex, a putative potassium–CK interaction involved in regulating both processes merits further attention.
- Published
- 2009
50. Hormonal changes during salinity-induced leaf senescence in tomato (Solanum lycopersicum L.).
- Author
-
UCL - SC/BIOL - Département de biologie, Ghanem, Michel Edmond, Albacete, Alfonso, Martínez-Andújar, Cristina, Acosta, Manuel, Romero-Aranda, Remedios, Dodd, Ian C., Lutts, Stanley, Pérez-Alfocea, Francisco, UCL - SC/BIOL - Département de biologie, Ghanem, Michel Edmond, Albacete, Alfonso, Martínez-Andújar, Cristina, Acosta, Manuel, Romero-Aranda, Remedios, Dodd, Ian C., Lutts, Stanley, and Pérez-Alfocea, Francisco
- Abstract
Leaf senescence is one of the most limiting factors to plant productivity under salinity. Both the accumulation of specific toxic ions (e.g. Na(+)) and changes in leaf hormone relations are involved in the regulation of this process. Tomato plants (Solanum lycopersicum L. cv Moneymaker) were cultivated for 3 weeks under high salinity (100 mM NaCl) and leaf senescence-related parameters were studied during leaf development in relation to Na(+) and K(+) contents and changes in abscisic acid (ABA), cytokinins, the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC), and the auxin indole-3-acetic acid (IAA). Na(+) accumulated to a similar extent in both leaves 4 and 5 (numbering from the base of the plant) and more quickly during the third week, while concurrently K(+) contents sharply decreased. However, photosystem II efficiency, measured as the F(v)/F(m) ratio, decreased from the second week of salinization in leaf 4 but only at the end of the third week in the younger leaf 5. In the prematurely senescent leaf 4, ABA content increased linearly while IAA strongly decreased with salinization time. Although zeatin (Z) levels were scarcely affected by salinity, zeatin-riboside (ZR) and the total cytokinin content (Z+ZR) progressively decreased by 50% from the imposition of the stress. ACC was the only hormonal compound that increased in leaf tissue coincident with the onset of oxidative damage and the decline in chlorophyll fluorescence, and prior to massive Na(+) accumulation. Indeed, (Z+ZR) and ACC contents and their ratio (Z+ZR/ACC) were the hormonal parameters best correlated with the onset and progression of leaf senescence. The influence of different hormonal changes on salt-induced leaf senescence is discussed.
- Published
- 2008
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