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Root-synthesized cytokinins improve shoot growth and fruit yield in salinized tomato (Solanum lycopersicum L.) plants.

Authors :
UCL - SST/ELI/ELIA - Agronomy
Ghanem, Michel Edmond
Albacete, Alfonso
Smigocki, Ann C
Frébort, Ivo
Pospísilová, Hana
Martínez-Andújar, Cristina
Acosta, Manuel
Sánchez-Bravo, José
Lutts, Stanley
Dodd, Ian C.
Pérez-Alfocea, Francisco
UCL - SST/ELI/ELIA - Agronomy
Ghanem, Michel Edmond
Albacete, Alfonso
Smigocki, Ann C
Frébort, Ivo
Pospísilová, Hana
Martínez-Andújar, Cristina
Acosta, Manuel
Sánchez-Bravo, José
Lutts, Stanley
Dodd, Ian C.
Pérez-Alfocea, Francisco
Source :
Journal of experimental botany, Vol. 62, no. 1, p. 125-140 (2011)
Publication Year :
2011

Abstract

Salinity limits crop productivity, in part by decreasing shoot concentrations of the growth-promoting and senescence-delaying hormones cytokinins. Since constitutive cytokinin overproduction may have pleiotropic effects on plant development, two approaches assessed whether specific root-localized transgenic IPT (a key enzyme for cytokinin biosynthesis) gene expression could substantially improve tomato plant growth and yield under salinity: transient root IPT induction (HSP70::IPT) and grafting wild-type (WT) shoots onto a constitutive IPT-expressing rootstock (WT/35S::IPT). Transient root IPT induction increased root, xylem sap, and leaf bioactive cytokinin concentrations 2- to 3-fold without shoot IPT gene expression. Although IPT induction reduced root biomass (by 15%) in control (non-salinized) plants, in salinized plants (100?mM NaCl for 22?d), increased cytokinin concentrations delayed stomatal closure and leaf senescence and almost doubled shoot growth (compared with WT plants), with concomitant increases in the essential nutrient K(+) (20%) and decreases in the toxic ion Na(+) (by 30%) and abscisic acid (by 20-40%) concentrations in transpiring mature leaves. Similarly, WT/35S::IPT plants (scion/rootstock) grown with 75?mM NaCl for 90?d had higher fruit trans-zeatin concentrations (1.5- to 2-fold) and yielded 30% more than WT/non-transformed plants. Enhancing root cytokinin synthesis modified both shoot hormonal and ionic status, thus ameliorating salinity-induced decreases in growth and yield.

Details

Database :
OAIster
Journal :
Journal of experimental botany, Vol. 62, no. 1, p. 125-140 (2011)
Notes :
English
Publication Type :
Electronic Resource
Accession number :
edsoai.on1130571124
Document Type :
Electronic Resource