93 results on '"Longobardi C"'
Search Results
2. Editorial: Towards 2030: Sustainable Development Goal 16: Peace, justice, and strong institutions. An educational perspective
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Fabris, M. A. and Longobardi, C.
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peace ,educational psychology ,justice ,Sustainable Development Goal (SDG) ,toward 2030 ,Education - Published
- 2023
- Full Text
- View/download PDF
3. Editorial: Student-teacher relationship quality research: Past, present and future
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Fabris, Ma, Roorda, D, Longobardi, C, and Developmental Disorders and Special Education (RICDE, FMG)
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academic achievement ,psychological adjustment ,editorial ,student-teacher relationships ,school-environment relations ,Education - Abstract
More than 20 years have passed since the publication of Pianta (2001) on the quality of the teacher-student relationship. Since then, several attempts have been made to elaborate theoretically the concept of teacher-student relationship quality and to provide empirical evidence of the impact that good teacher-student relationship quality might have on academic achievement, student psychological adjustment, and classroom climate. The teacher has been recognized as a “psychological parent” and defined as a secure base and safe heaven, following attachment theory (Verschueren and Koomen, 2012, 2021; Prino et al., 2022; Spilt et al., 2022). Several studies have shown that a relationship with the teacher characterized by affection, closeness, and respect predicts more favorable developmental outcomes and better adjustment to the classroom context in any school setting (Roorda et al., 2011, 2017; Longobardi et al., 2019, 2021; Lin et al., 2022). However, after 20 years, we saw the need to synthesize the current literature on the topic of teacher-learner relationship quality and to promote a collection of studies that provide new insights, ideas, and reflections to advance the research field and overcome current limitations.In this Research Topic, 16 publications were collected from different parts of the world. The Research Topic includes two literature reviews, several empirical works, some of which aim to develop and validate instruments to measure the quality of the teacher-student relationship, and others to promote new knowledge about the effects and mechanisms of action of the quality of the teacher-learner relationship on the psychological development and adjustment processes of children and adolescents. In addition, the Research Topic includes a contribution on possible intervention strategies on the quality of teacher-student relationship.
- Published
- 2022
4. Improvement of communicative-pragmatic ability in adolescents with autism spectrum disorder:the adapted version of the cognitive pragmatic treatment
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Gabbatore, I. (Ilaria), Longobardi, C. (Claudio), Bosco, F. M. (Francesca M.), Gabbatore, I. (Ilaria), Longobardi, C. (Claudio), and Bosco, F. M. (Francesca M.)
- Abstract
Autism spectrum disorder (ASD) is a complex pathology that includes impaired social interaction abilities. Insufficient attention has been paid to programs specifically devoted to improving communicative-pragmatic skills. Moreover, the majority of studies have focused on children, while programs specifically developed for the adolescents are lacking. The present study aims to test the feasibility and acceptability of the adapted version of the Cognitive Pragmatic Treatment for adolescents (A-CPT), a 15-session group training, as well as its ability to improve the communicative-pragmatic performance of adolescents with ASD. Twenty-one verbally fluent adolescents with ASD took part in the training; they were assessed in three phases, i.e., before, after and at three-month follow-up, using the equivalent forms of the Assessment Battery for Communication (ABaCo), a tool for testing a wide range of pragmatic phenomena, such as direct and indirect speech acts, irony, deceit and violation of Grice’s maxims, expressed through linguistic, non-verbal, i.e., gestures, or paralinguistic expressive means. Furthermore, Theory of Mind (ToM) tasks and tests investigating the main cognitive domains, for example, Executive Functions (planning, shifting, working memory) and long-term memory, were administered. The results showed an improvement in participants’ performance in all the four scales of the ABaCo, i.e., linguistic, extralinguistic, paralinguistic and context scale; this improvement was maintained at follow-up assessment three months after the end of the program. No improvement was observed in the cognitive and ToM domains investigated, with the only exception of expressive vocabulary task. Despite the lack of a control group, the high degree of feasibility of the CPT, highlight the importance of more work needed in this research line.
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- 2022
5. THE REPRESENTATION OF MALE AND FEMALE GENDER ROLE DEVELOPMENT IN CHILDREN’S DRAWINGS: AN EXAMINATION OF 20 YEARS OF CHANGES IN ITALIAN CULTURE AND SOCIETY
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Longobardi, C., Bozzato, P., and Fabris, M. A.
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Classroom drawing ,Children’s drawings ,Gender roles ,Italian schoolchildren - Published
- 2022
6. Rediscovering Teaching in University: A Scoping Review of Teacher Effectiveness in Higher Education
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Mastrokoukou, S. Kaliris, A. Donche, V. Chauliac, M. Karagiannopoulou, E. Christodoulides, P. Longobardi, C.
- Abstract
Although teacher effectiveness plays a critical role in the learning process, little is known about its conceptualization and assessment, particularly in higher education (HE). This review aims to fill this gap by (a) listing the literature on teacher effectiveness, (b) identifying the instruments that have been used to assess teacher effectiveness (HE), and (c) highlighting the most effective teaching approaches based on the relevant literature. The selection process considered studies published since 1990 and conducted in higher education contexts with students. The research articles measured instructional processes and faculty effectiveness in terms of student outcomes, focusing on student achievement and student satisfaction. In reviewing the international research, special attention was paid to Southeastern Europe and Greece in particular. After a thorough review, the analysis revealed 26 studies. The results show that there is no universal definition of effective higher education teaching. Effective teaching may manifest itself in high scores on student performance assessments or in rewarding classroom interactions. Based on this principle, the way teacher effectiveness is defined is closely linked to proposed solutions in educational policy. Furthermore, research has shown that student-centered teaching styles are perceived by students as more effective, engaging, and performance-enhancing. However, several studies have not clarified why different teachers use different teaching styles in similar contexts. This review represents a step forward in our understanding of teacher effectiveness in HE. Nonetheless, effective teaching strategies could be better conceptualized through future research aimed at assessing the contextual nature of teaching along with student perceptions of effectiveness and expectations for an effective classroom climate. Copyright © 2022 Mastrokoukou, Kaliris, Donche, Chauliac, Karagiannopoulou, Christodoulides and Longobardi.
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- 2022
7. CHILDREN PET OWNERSHIP: ATTACHMENT TO PARENTS AND PSYCHOLOGICAL ADJUSTMENT
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Longobardi, C., Ribera, L. B., Prino, L. E., and Fabris, M. A.
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human-animal interaction ,attachment to parents ,pet ,children’s adjustment - Published
- 2022
8. Curcumin Modulates Nitrosative Stress, Inflammation, and DNA Damage and Protects against Ochratoxin A-Induced Hepatotoxicity and Nephrotoxicity in Rats
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Longobardi C, Damiano S, Andretta E, Prisco F, Russo V, Pagnini F, Florio S, Ciarcia R., Longobardi, C, Damiano, S, Andretta, E, Prisco, F, Russo, V, Pagnini, F, Florio, S, and Ciarcia, R.
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hepatotoxicity ,inflammation ,nephrotoxicity ,nitrosative stre ,DNA damage ,ochratoxin A - Abstract
Ochratoxin A (OTA) is a fungal toxin of critical concern for food safety both for human health and several animal species, also representing a cancer threat to humans. Curcumin (CURC) is a natural polyphenol that has anti-apoptotic, anti-inflammatory, and antioxidant effects. The aim of this study was to investigate the cytoprotective effect of CURC against OTA-induced nephrotoxicity and hepatotoxicity through the study of the nitrosative stress, pro-inflammatory cytokines, and deoxyribonucleic acid (DNA) damage. Sprague Dawley rats were daily treated with CURC (100 mg/kg b.w.), OTA (0.5 mg/kg b.w), or CURC with OTA by oral gavage for 14 days. Our results demonstrated that OTA exposure was associated with significant increase of pro-inflammatory and DNA oxidative-damage biomarkers. Moreover, OTA induced the inducible nitric oxide synthase, (iNOS) resulting in increased nitric oxide (NO) levels both in kidney and liver. The co-treatment OTA + CURC counteracted the harmful effects of chronic OTA treatment by regulating inflammation, reducing NO levels and oxidative DNA damage in kidney and liver tissues. Histology revealed that OTA + CURC treatment determinates mainly an Iba1+ macrophagic infiltration with fewer CD3+ T-lymphocytes in the tissues. In conclusion, we evidenced that CURC exerted cytoprotective and antioxidant activities against OTA-induced toxicity in rats.
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- 2021
9. Potential Approaches Versus Approved or Developing Chronic Myeloid Leukemia Therapy
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Andretta E, Costa C, Longobardi C, Damiano S, Giordano A, Pagnini F, Montagnaro S, Quintiliani M, Lauritano C, Ciarcia R., Andretta, E, Costa, C, Longobardi, C, Damiano, S, Giordano, A, Pagnini, F, Montagnaro, S, Quintiliani, M, Lauritano, C, and Ciarcia, R.
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antioxidant ,chronic myeloid leukemia ,oncolytic therapy ,exosome ,marine organism ,Non-BCR-ABL targeted drug ,tyrosine kinase inhibitors ,quiescent leukemia stem cell - Abstract
Tyrosine kinase inhibitors (TKIs) have revolutionized the treatment of patients with chronic myeloid leukemia (CML). However, continued use of these inhibitors has contributed to the increase in clinical resistance and the persistence of resistant leukemic stem cells (LSCs). So, there is an urgent need to introduce additional targeted and selective therapies to eradicate quiescent LSCs, and to avoid the relapse and disease progression. Here, we focused on emerging BCR-ABL targeted and non-BCR-ABL targeted drugs employed in clinical trials and on alternative CML treatments, including antioxidants, oncolytic virus, engineered exosomes, and natural products obtained from marine organisms that could pave the way for new therapeutic approaches for CML patients.
- Published
- 2021
10. 1563P Tumor-intrinsic subtypes of esophageal adenocarcinoma associate cellular phenotypes with responses to therapy
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Blangé, D., Dings, M., van der Zalm, A., Lansbergen, M., Verzaal, M., Oyarce, C., Meijer, S., Waasdorp, C., van Mourik, M., Longobardi, C., Cuppen, E., Bloemendal, H., Koster, J., Zwijnenbrug, D., Medema, J-P., van Laarhoven, H.W.M., and Bijlsma, M.F.
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- 2023
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11. The Role of Body Image Concerns in Online Sexual Victimization among Female Adolescents: The Mediating Effect of Risky Online Behaviors
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Longobardi, C, primary, Fabris, M A, additional, Prino, L E, additional, and Settanni, M, additional
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- 2020
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12. Sibling Relationships, Personality Traits, Emotional, and Behavioral Difficulties in Autism Spectrum Disorders
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Longobardi, C., primary, Prino, L. E., additional, Gastaldi, F. G. M., additional, and Jungert, T., additional
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- 2019
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13. Phase II study of cetuximab in combination with FOLFIRI in patients with untreated advanced gastric or gastroesophageal junction adenocarcinoma (FOLCETUX study)
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Pinto, C., Di Fabio, F., Siena, S., Cascinu, S., Llimpe, F. L. Rojas, Ceccarelli, C., Mutri, V., Giannetta, L., Giaquinta, S., Funaioli, C., Berardi, R., Longobardi, C., Piana, E., and Martoni, A. A.
- Published
- 2007
14. Measuring the influence of stress and burnout in teacher-child relationship
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Longobardi, C., Quaglia, R., Gastaldi, F. G., Prino, L. E., and Pasta, T.
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Teacher-child reationship ,bornout ,self - efficacy ,education ,lcsh:BF1-990 ,teacher burn out ,Stress ,lcsh:LB5-3640 ,teacher effectivenes ,lcsh:Theory and practice of education ,lcsh:Psychology ,School Psychology ,Teacher - child interaction ,lcsh:L ,psychological phenomena and processes ,lcsh:Education - Abstract
Most research finds socio-psychological aspects and the organism-environment relationship crucial in the onset of stress-related phenomena; equally important is the subject’s evaluation of stimuli. The Burnout syndrome is defined as a psychological state perceived as emotive breakdown and sense of depersonalization, featuring decreased effectiveness at work and a lower evaluation of one’s performance. At school, the most significant widespread stressors are issues in managing student behavior. However, in the literature few works treat stress and burnout as a specific function of the teacher-pupil relationship. This research will highlight the significant correlations existing between burnout, self-efficacy, students’ academic performance and the quality of pupil-teacher relationships. 37 primary-school teachers in north-west Italy were involved (age range: 27-57 yrs; M=44.76 yrs; SD=8.38 yrs). Tools used were a specially designed socio-personal questionnaire, the Maslach Burnout Inventory (M.B.I.) and the Classroom and School Context Teacher Self-Efficacy Scale (CSC-TSES), while teachers’ perception of the relationship with their pupils was investigated with STRS (Italian adaptation). Preliminary analysis reveals mutual influence between teachers' self-efficacy levels and degree of burnout; Conflict is the only dimension correlating significantly with burnout levels and self-efficacy in class management ability
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- 2014
15. Social cognition in people with schizophrenia: a cluster-analytic approach
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Rocca, P., Galderisi, S., Rossi, A., Bertolino, A., Rucci, P., Gibertoni, D., Montemagni, C., Sigaudo, M., Mucci, A., Bucci, P., Acciavatti, T., Aguglia, E., Amore, M., Bellomo, A., Deronchi, D., Osso, L., Difabio, F., Girardi, P., Goracci, A., Marchesi, C., Monteleone, P., Niolu, C., Pinna, F., Roncone, R., EMILIO SACCHETTI, Santonastaso, P., Zeppegno, P., Maj, M., Chieffi, M., Piegari, M., Vignapiano, A., Merlotti, E., Plescia, G., Montefusco, V., Bava, I., Mancini, I., Sandei, L., Antoniettanettis, I., Rizzo, G., Mancini, M., Porcelli, S., Salfi, G., Bianchini, O., Antonio Vita, Galluzzo, G., Barlati, S., Carpiniello, B., Primavera, D., Floris, S., Salvina, Signorelli, Minutolo, B., Cannavò, G., Corbo, D., Vellante, M., Alessandrini, F., Poli, M., Altamura, M., Petito, M., Marasco, A., Vaggi, D., Calcagno, M., Marozzi, P., Ussorio, V., Giusti, D., Malavolta, L., Diemidio, M., Stratta, G., Collazzoni, P., Debartolomeis, P., Gramaglia, P., Gili, C., Gattoni, S., Ferronato, E., Giannunzio, L., Tenconi, V., Tonna, E., Ossola, M., Camerlengo, P., Landi, E., Rutigliano, P., Buzzanca, G., Paolemili, A., Frascarelli, M., Comparelli, M., Corigliano, A., Brugnoli, V., Siracusano, R., Troisi, A., Dilorenzo, A., Filippo, Di, Longobardi, C., Castaldo, N., Fagiolini, E., Bolognesi, A., Capua, De, A, DIPARTIMENTO DI SCIENZE BIOMEDICHE E NEUROMOTORIE, Facolta' di MEDICINA e CHIRURGIA, AREA MIN. 06 - Scienze mediche, Da definire, Rocca, P, Galderisi, S., Rossi, A., Bertolino, A., Rucci, P., Gibertoni, D., Montemagni, C., Sigaudo, M., Mucci, A., Bucci, P., Acciavatti, T., Aguglia, E., Amore, M., Bellomo, A., De Ronchi, D., Dell'Osso, L., Di Fabio, F., Girardi, P., Goracci, A., Marchesi, C., Monteleone, P., Niolu, C., Pinna, F., Roncone, R., Sacchetti, E., Santonastaso, P., Zeppegno, P., Maj, M., Rocca, P., Chieffi, M., Piegari, M., Vignapiano, A., Merlotti, E., Plescia, G., Montefusco, V., Bava, I., Mancini, I., Sandei, L., Antonietta Nettis, I., Rizzo, G., Mancini, M., Porcelli, S., Salfi, G., Bianchini, O., Vita, A., Galluzzo, G., Barlati, S., Carpiniello, B., Primavera, D., Floris, S., Salvina Signorelli, B., Minutolo, G., Cannavo, D., Corbo, M., Vellante, F., Alessandrini, M., Poli, M., Altamura, M., Petito, A., Marasco, D., Vaggi, M., Calcagno, P., Marozzi, V., Ussorio, D., Giusti, L., Malavolta, M., Di Emidio, G., Stratta, P., Collazzoni, P., De Bartolomeis, P., Gramaglia, C., Gili, S., Gattoni, E., Ferronato, L., Giannunzio, V., Tenconi, E., Tonna, M., Ossola, P., Camerlengo, E., Landi, P., Rutigliano, G., Buzzanca, A., Paolemili, M., Frascarelli, M., Comparelli, A., Corigliano, V., Brugnoli, R., Siracusano, A., Troisi, A., Di Lorenzo, G., Di Filippo, C., Longobardi, N., Castaldo, E., Fagiolini, A., Bolognesi, S., De Capua, A., and Italian Network for Research on, Psychoses
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Adult ,Male ,Context (language use) ,social cognition ,Italian Network for Research on Psychoses ,03 medical and health sciences ,0302 clinical medicine ,Cluster analysis ,Social cognition ,Emotion perception ,medicine ,schizophrenia ,theory of mind ,Cluster Analysis ,Emotional Intelligence ,Facial Recognition ,Female ,Humans ,Middle Aged ,Schizophrenia ,Facial Expression ,Social Perception ,Wit and Humor as Topic ,Applied Psychology ,Psychiatry and Mental Health ,Cluster analysis Italian Network for Research on Psychosesschizophrenia social cognition theory of mind ,Cluster analysi ,Italian Network for Research on Psychose ,Settore MED/25 - Psichiatria ,Facial expression ,Social perception ,Emotional intelligence ,medicine.disease ,cluster analysis ,italian network for research on psychoses ,adult ,emotional intelligence ,facial recognition ,female ,humans ,male ,middle aged ,facial expression ,social perception ,wit and humor as topic ,applied psychology ,psychiatry and mental health ,030227 psychiatry ,Psychology ,Neurocognitive ,030217 neurology & neurosurgery ,Clinical psychology - Abstract
none 28 no BACKGROUND: The study aimed to subtype patients with schizophrenia on the basis of social cognition (SC), and to identify cut-offs that best discriminate among subtypes in 809 out-patients recruited in the context of the Italian Network for Research on Psychoses. METHOD: A two-step cluster analysis of The Awareness of Social Inference Test (TASIT), the Facial Emotion Identification Test and Mayer-Salovey-Caruso Emotional Intelligence Test scores was performed. Classification and regression tree analysis was used to identify the cut-offs of variables that best discriminated among clusters. RESULTS: We identified three clusters, characterized by unimpaired (42%), impaired (50.4%) and very impaired (7.5%) SC. Three theory-of-mind domains were more important for the cluster definition as compared with emotion perception and emotional intelligence. Patients more able to understand simple sarcasm (⩾14 for TASIT-SS) were very likely to belong to the unimpaired SC cluster. Compared with patients in the impaired SC cluster, those in the very impaired SC cluster performed significantly worse in lie scenes (TASIT-LI
- Published
- 2016
16. Can the use of clinical decision support system (CDSS) affect the number of adverse drug reaction (ADR) reports in the oncological patient? A preliminary evaluation of the status quo in Reggio Emilia Oncological Center (CORE) and future perspectives
- Author
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longobardi, C., primary, Masini, L., additional, and D'Incà, M., additional
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- 2017
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17. L'immagine della consulenza psicologica presso gli insegnanti della scuola italiana
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GUIDI, MARCO, SALVATORE, Sergio, Pasta T., Longobardi C., Guidi, Marco, Pasta, T., Longobardi, C., and Salvatore, Sergio
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Psicologia scolastica ,Immagine Consulenza Psicologica ,Formazione degli Insegnanti - Abstract
Il presente lavoro riporta i risultati di una analisi del modo con cui i docenti della scuola italiana si rappresentano la consulenza psicologica rivolta al mondo scolasti- co. La ricerca ha adottato 829 insegnanti delle scuole primarie, superiori di primo e di secondo grado del Nord, Centro e Sud Italia a cui è stato proposto un questionario predisposto ad hoc, concernente diverse dimensioni del mondo scolastico e della funzione psicologica. Le risposte ottenute sono state sottoposte ad una procedura di analisi multidimensionale (Analisi delle Corrispondenze Multiple e Analisi dei Cluster), che ha portato ad enucleare uno Spazio Simbolico generato da due strutture latenti di senso e 5 differenti Immagini della Consulenza, ciascuna delle quali indi- cativa di uno specifico modo di connotare la funzione psicologica scolastica.
- Published
- 2009
18. Resilienza e punti di forza e di debolezza del comportamento del bambino nel contesto scolastico
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Prino, L. E., Quaglia, R., Longobardi, C., Gastaldi, F. G., and Pasta, T.
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Teacher-child relationship ,RESILIENZA ED EDUCAZIONE - Published
- 2014
19. Niveles de percepción del sentido de eficacia en la gestión del contexto escolar y de la clase en una muestra de docentes de la escuela italiana
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Pasta, T, Longobardi, C, Guidi, M, Salvatore, S, Pasta, T, Longobardi, C, Guidi, Marco, and Salvatore, Sergio
- Abstract
Una vez configuradas las convicciones de eficacia como los principales predictores del éxito organizativo (Stajcovic & Luthans, 1998) y reconocido el concepto de autoeficacia percibida como un sistema de creencias determinantes (Caprara, 2001) que sirve de apoyo y de moderador a las acciones que la persona efectúa (Bandura,1999), este trabajo ofrece una visión del modo en el que 829 docentes, en 9 regiones italianas, perciben y describen su propia eficacia en la función formativa. Con la ayuda de la escala de “Autoeficacia de clase y escolar percibida” (CSC scale) de I.A. Friedmann y E. Kass (2002) y con la realización del análisis factorial de los principales componentes que han permitido verificar la percepción de los profesores en relación con su propia experiencia en la enseñanza, de manera separada sobre la base de la gestión de la clase y de la situación escolar en su conjunto, emerge que los docentes se consideran más eficaces en la relación diádica con los alumnos que con los actores y acciones educativas generales de la vida escolar, incluso demostrando niveles de eficacia más bien altos respecto a ambas escalas. Se han realizado análisis detallados de la percepción de la autoeficacia con el objetivo de comprobar el papel que juegan algunas variables socio-culturales y demográficas destancando diferencias significativas respecto a la pertenencia al nivel escolar y al área regional, lo que ha inducido a la verificación de la hipótesis de una influencia ejercitada por variables de contexto más que por variables inherentes a factores personales.
- Published
- 2008
20. The Effect of Autism Spectrum Disorders, Down Syndrome, Specific Learning Disorders and Hyperactivity and Attention Deficits on the Student-Teacher Relationship
- Author
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Prino, L.E., primary, Pasta, T., additional, Gastaldi, F., additional, and Longobardi, C., additional
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- 2016
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21. Health and physical activity: the effect of outdoor play on children's motor development
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Gastaldi, F. G., Prino, L. E., Quaglia, R., and Longobardi, C.
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- 2013
22. Emotional development in late childhood: interaction between socio-relational and self-regulation factors in pupils of primary school
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Gastaldi, F. G., Longobardi, C., Pasta, T., and Quaglia, R.
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self-regulation ,social behaviour ,intimate friendship - Published
- 2012
23. T15 - Can the use of clinical decision support system (CDSS) affect the number of adverse drug reaction (ADR) reports in the oncological patient? A preliminary evaluation of the status quo in Reggio Emilia Oncological Center (CORE) and future perspectives
- Author
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longobardi, C., Masini, L., and D'Incà, M.
- Published
- 2017
- Full Text
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24. [Gestational diabetes. Determination of relative importance of risk factors]
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Gs, Etchegoyen, Er, Martini, Parral Longobardi C, Cédola N, Alvariñas J, González C, and Jj, Gagliardino
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Adult ,Diabetes, Gestational ,Logistic Models ,Pregnancy ,Risk Factors ,Case-Control Studies ,Multivariate Analysis ,Argentina ,Odds Ratio ,Humans ,Female ,Obesity ,Retrospective Studies - Abstract
The aim of this study was to determine the relative importance of gestational diabetes (GD) risk factors to identify populations at risk. A total of 400 pregnant patients were studied, 200 with confirmed GD diagnosis and 200 controls with risk factors. They regularly attended health-care units belonging to the Ministry of Health of the Province of Buenos Aires, in the context of the Program for the Prevention, Care and Treatment of People with Diabetes of the Province of Buenos Aires (PRODIABA). The following risk factors were evaluated: GD in previous pregnancies, history of diabetes in first degree relatives, ageor = 30 years, BMI26, history of fetal macrosomy, perinatal mortality and hypertension during pregnancy. Data analysis was performed with the Program of Statistics in Public Health Epilnfo 6. The association between GD development as a dependent variable and the presence of different risk factors (independent variables) was analyzed with a multiple logistic regression model, determining the logistic probability to develop GD. Results showed that the incidence of risk factors to develop GD is not the same; therefore, they do not have the same predictive value. Overweight or obesity played a key central role, not only for its frequency, but also for its contribution to GD development. Our findings reinforce the importance of multi causal studies as the basis to design and implement prevention strategies for diabetes.
- Published
- 2001
25. Curcumin Supplementation Protects Broiler Chickens Against the Renal Oxidative Stress Induced by the Dietary Exposure to Low Levels of Aflatoxin B1
- Author
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Sara Damiano, Watanya Jarriyawattanachaikul, Flavia Girolami, Consiglia Longobardi, Carlo Nebbia, Emanuela Andretta, Chiara Lauritano, Sihem Dabbou, Giuseppina Avantaggiato, Achille Schiavone, Paola Badino, Roberto Ciarcia, Damiano, S., Jarriyawattanachaikul, W., Girolami, F., Longobardi, C., Nebbia, C., Andretta, E., Lauritano, C., Dabbou, S., Avantaggiato, G., Schiavone, A., Badino, P., and Ciarcia, R.
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2. Zero hunger ,chicken--broiler ,0303 health sciences ,oxidative stre ,kidney ,Aflatoxin B1 ,General Veterinary ,Veterinary medicine ,030302 biochemistry & molecular biology ,renal damage ,chicken—broiler ,curcumin ,oxidative stress ,turmeric powder ,Aflatoxin B1, turmeric powder, curcumin, oxidative stress, kidney, chicken—broiler, renal damage ,03 medical and health sciences ,SF600-1100 ,Veterinary Science ,Original Research ,030304 developmental biology - Abstract
Aflatoxin B1 (AFB1) causes hepatotoxicity, immunotoxicity, and kidney damage, and it is included in group I of human carcinogens. The European Commission has established maximum limits of AFB1 in feed, ranging from 5 to 20 μg/kg. Chicken is moderately sensitive to AFB1, which results in reduced growth performance and economic losses. Oxidative stress triggered by AFB1 plays a crucial role in kidney damage and the antioxidant activity of Curcumin (CURC) could help in preventing such adverse effect. Twenty-days-old broilers were treated for 10 days with AFB1 (0.02 mg/kg feed), alone or in combination with CURC (400 mg/kg feed), to explore the effects on the renal tissue. Animals exposed to AFB1 alone displayed alterations of the oxidative stress parameters compared with controls: serum antioxidant capacity, and enzymatic activity of kidney superoxide dismutase, catalase and glutathione peroxidase were decreased, while renal malondialdehyde levels and NADPH oxidase complex expression were increased. The administration of CURC attenuates all the oxidative stress parameters modified by AFB1 in the chicken kidney, opening new perspectives in the management of aflatoxicosis.
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- 2022
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26. Aujeszky’s Disease in South-Italian Wild Boars (Sus scrofa): A Serological Survey
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Sara Damiano, Roberto Ciarcia, Valentina Iovane, Giuseppe Iovane, Ugo Pagnini, Consiglia Longobardi, Nicola D’Alessio, Gianmarco Ferrara, Maria Grazia Amoroso, Serena Montagnaro, Filomena D’Ambrosi, Ferrara, G., Longobardi, C., D'Ambrosi, F., Amoroso, M. G., D'Alessio, N., Damiano, S., Ciarcia, R., Iovane, V., Iovane, G., Pagnini, U., and Montagnaro, S.
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Veterinary medicine ,endocrine system ,serosurvey ,animal diseases ,viruses ,Pseudorabies ,Virus ,Article ,Serology ,Hunting season ,Wild boar ,biology.animal ,SF600-1100 ,Seroprevalence ,Aujeszky’s disease ,General Veterinary ,biology ,virus diseases ,biology.organism_classification ,Domestic pig ,QL1-991 ,Animal Science and Zoology ,ELISA ,Viral disease ,wild boars ,Southern Italy ,Zoology - Abstract
Aujeszky’s disease (AD, pseudorabies) is a viral disease of suids caused by Suid Herpesvirus 1 (SHV-1) also referred as Aujeszky’s disease virus (ADV) or Pseudorabies virus (ADV). Domestic pig and Wild boar (Sus scrofa) are the natural host, but many species can be infected with ADV. The aim of our study was to evaluate seroprevalence of AD in wild boar hunted in the Campania Region, during the 2016–2017 hunting season. A total of 503 serum samples from wild boars hunted in the provinces of Campania Region (Southern Italy) were collected and were tested for antibody against ADV using an AD, blocking ELISA assay. A Seroprevalence of 23.85% (120/503, 95% Confidence Interval (CI): 20.15–27.55) was found. Gender was not significantly associated with of ADV seropositivity (p >, 0.05), while the presence of ADV antibodies was statistically associated with age (>, 36-month, p <, 0.0001) and location (Avellino, p = 0.0161). Our prevalence values are like those obtained in 2010 in our laboratory (30.7%), demonstrating a constant circulation of ADV in the area.
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- 2021
27. Phase II study of cetuximab in combination with FOLFIRI in patients with untreated advanced gastric or gastroesophageal junction adenocarcinoma (FOLCETUX study)
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S. Giaquinta, Claudio Ceccarelli, V. Mutri, Stefano Cascinu, F. Di Fabio, Rossana Berardi, Piana E, Laura Giannetta, Salvatore Siena, C. Funaioli, C. Longobardi, Carmine Pinto, Andrea Martoni, F. L. Rojas Llimpe, Pinto C, Di Fabio F, Siena S, Cascinu S, Rojas Llimpe FL, Ceccarelli C, Mutri V, Giannetta L, Giaquinta S, Funaioli C, Berardi R, Longobardi C, Piana E, and Martoni AA
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Adult ,Male ,medicine.medical_specialty ,Time Factors ,Esophageal Neoplasms ,ADVANCED GASTRIC CANCER ,EGFR ,Leucovorin ,Phases of clinical research ,Kaplan-Meier Estimate ,Adenocarcinoma ,Gastroesophageal Junction Adenocarcinoma ,Neutropenia ,Antibodies, Monoclonal, Humanized ,Gastroenterology ,Drug Administration Schedule ,TARGETED THERAPY ,Stomach Neoplasms ,Internal medicine ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,FOLFIRI Regimen ,Humans ,Karnofsky Performance Status ,Stomach cancer ,FOLFIRI REGIMEN ,Aged ,Aged, 80 and over ,Cetuximab ,business.industry ,Antibodies, Monoclonal ,Hematology ,Middle Aged ,medicine.disease ,CETUXIMAB ,Surgery ,Treatment Outcome ,Italy ,Oncology ,Fluorouracil ,Positron-Emission Tomography ,FOLFIRI ,Camptothecin ,Female ,Esophagogastric Junction ,Tomography, X-Ray Computed ,business ,medicine.drug - Abstract
Background: The purpose of this phase II study was to evaluate the efficacy and safety of cetuximab combined with FOLFIRI as a first-line treatment of advanced gastric or gastroesophageal junction (GEJ) adenocarcinoma. Patients and methods: Untreated patients with confirmed advanced gastric or gastroesophageal adenocarcinoma received cetuximab at an initial dose of 400 mg/m2 intravenously (i.v.) followed by weekly doses of 250 mg/m2, CPT 11 180 mg/m2 i.v. on day 1, LFA 100 mg/m2 i.v. followed by 5-FU 400 mg/m2 i.v. bolus, and 600 mg/m2 i.v. 22-h continuous infusion on days 1 and 2 (FOLFIRI) every 2 weeks, for a maximum of 24 weeks, then cetuximab alone was allowed in patients with a complete response, partial response, or stable disease. Antitumor activity was assessed by computed tomography (CT) and positron emission tomography (PET) at baseline and after 6 weeks, and further by CT alone or CT and PET every 6 weeks. Results: Thirty-eight patients were enrolled (median age 63.5 years, range 39–83; median Karnofsky performance status 90, range 70–100; stomach 89.5% and GEJ 10.5%; locally advanced disease 13.2% and metastatic disease 86.8%). All 38 patients were assessed for safety and survival, and 34 patients were assessed for overall response rates (ORR). The ORR was 44.1% [95% confidence interval (CI) 27.5% to 60.9%]. The median time-to-progression was 8 months (95% CI 7–9). At the median follow-up time of 11 months, 55.3% of patients were alive, with a median expected survival time of 16 months (95% CI 9–23). Grade 3–4 toxicity included neutropenia (42.1%), acne-like rash (21.1%), diarrhea (7.9%), asthenia (5.3%), stomatitis (5.3%), and hypertransaminasemia (5.3%). There was one (2.6%) treatment-related death. Conclusions: The combination of cetuximab and FOLFIRI is active in gastric and GEJ adenocarcinoma. The higher toxicity appears to be limited to neutropenia.
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- 2007
28. 'Manuali brevi' di metrica latina e caratteristiche d'autore. Con anticipazioni sul 'De arte metrica' di Marziano Capella
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DE NONNO, Mario, Longobardi C, Nicolas Chr, Squillante M, and DE NONNO, Mario
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Mallio Teodoro ,Atilio Fortunaziano ,Metricologi latini ,Grammatici latini ,Marziano Capella ,Servio Onorato - Abstract
Viene analizzata la tipologia del «manuale breve di metrica latina», nelle sue quattro occorrenze tardoantiche: il «De metrica Horatianis» di Atilio Fortunaziano, il «De metris» di Mallio Teodoro, il «Centimeter» di Servio e l'inedito «De arte metrica» attribuito a Marziano Capella. Di ciascun testo si individuano, attraverso un'opportuna contestualizzazione storica e dottrinale, e una valutazione comparativa, la fisionomia e gli intenti. Del «De arte metrica», in particolare, vengono per la prima volta pubblicati criticamente, inquadrati dottrinalmente e commentati tre passi di rilievo esemplare.
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- 2014
29. Nanocarrier-Based Drug Delivery Systems Targeting Kidney Diseases.
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Zucaro L, Longobardi C, Miele A, Villanova A, and Suzumoto Y
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Background: The potential applications of nanotechnology in the medical field have become increasingly recognized in recent years. Nanocarriers have emerged as a versatile tool, offering a wide range of applications due to their unique properties. In addition to the targeted drugs delivery, nanocarriers have also proven to be extremely effective in imaging and diagnostics. Continuous advances in nanotechnology have paved the way for innovative solutions to complex challenges in human health, shaping the future of nanotechnology and its applications., Summary: By exploring different types of nanoparticles, this review delves into the different characteristics that can be tailored to enhance their kidney access. Although the structural complexity of the kidney may prevent nanocarriers passage, optimization of nanocarrier characteristics such as shape, size, charge, and surface modifications may overcome these barriers, allowing for targeted delivery. By harnessing the potential of nanoparticles, researchers aim to develop targeted and efficient therapies that can address various kidney-related disorders., Key Messages: This review highlights the promising advancements in nanotechnology and their potential impact on improving the therapeutic outcomes for several kidney diseases., (The Author(s). Published by S. Karger AG, Basel.)
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- 2024
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30. Interoceptive sensibility and alcohol craving in Polish prisoners: the role of alexithymia and emotional dysregulation.
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Ścigała DK, Fabris MA, Zdankiewicz-Ścigała E, Kuc K, and Longobardi C
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Introduction: Alcohol craving, characterized by a strong desire or compulsion to consume alcohol, is a prominent symptom of substance dependence syndrome. Research indicates that alcohol craving is a significant factor leading to the termination of abstinence. The mechanisms underlying the activation of alcohol craving remain not fully understood. The urge to reach for alcohol may be stimulated by emotions, memories, thoughts, or bodily sensations, as well as external factors. It has been postulated that individuals with high levels of interoceptive sensibility tend to exhibit a high degree of alexithymia and emotion dysregulation in the context of alcohol craving. Deficits in identifying and verbalizing emotions, along with an operational thinking style, facilitate alcohol consumption by impeding accurate insight into one's mental state, thereby hindering the comprehension of bodily states, emotions, and the regulation of self., Method: This study involved 160 inmates incarcerated in a prison in Poland, awaiting participation in therapy for individuals with substance dependence following psychiatric diagnosis. Four questionnaires were used in the study: multidimensional Assessment of Interoceptive Sensibility (MAIA) for interoceptive sensibility, Toronto Alexithymia Scale (TAS-20) for alexithymia, Difficulties in Emotion Regulation Scale (DERS) for emotional dysregulation, and the Penn Alcohol Craving Scale (PACS) for alcohol craving assessment., Results: The results of the study are as follows: the study findings indicated that alexithymia and emotional dysregulation significantly mediates the relationship between interoceptive sensibility and alcohol craving. The indirect effect for both factors was found to be significant, similar to the indirect effect observed for alexithymia as an mediator. However, in the case of emotional dysregulation, no significant indirect effect was observed., Conclusion: Our study provides insights into the potential contribution of interoceptive sensibility to the heightened risk of alcohol dependence. Specifically, impaired interoceptive sensibility may be associated with the development of alexithymia and emotional dysregulation, potentially rendering individuals more susceptible to alcohol craving. Interoceptive sensibility could serve as a prerequisite for the cultivation of positive emotional processing skills., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision., (Copyright © 2024 Ścigała, Fabris, Zdankiewicz-Ścigała, Kuc and Longobardi.)
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- 2024
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31. Evaluation of 16S-Based Metagenomic NGS as Diagnostic Tool in Different Types of Culture-Negative Infections.
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Rimoldi SG, Tamoni A, Rizzo A, Longobardi C, Pagani C, Salari F, Matinato C, Vismara C, Gagliardi G, Cutrera M, and Gismondo MR
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Bacterial infections pose significant global health challenges, often underestimated due to difficulties in accurate diagnosis, especially when culture-based diagnostics fail. This study assesses the effectiveness of 16S-based metagenomic next generation sequencing (NGS) for identifying pathogens in culture-negative clinical samples across various medical settings. Overall, 48% of samples were collected from orthopedics, 15% from neurosurgery, and 12% in cardiac surgery, among others. The detection rate of monomicrobial infections was 68.6%, and 5.7% for polymicrobial infections. In addition, NGS detected bacteria in all samples from the lungs, head and neck, and eye specimens. Cutibacterium acnes (11%, 12/105) was the most frequent microorganism, followed by Staphylococcus epidermidis (10.4%, 11/105), and Staphylococcus aureus (9.5%, 10/105). In conclusion, 16S-targeted metagenomic sequencing enhances pathogen detection capabilities, particularly in instances where traditional cultures fail. By the combination of NGS and bacterial cultures, microbiologists might provide a more accurate diagnosis, guiding more effective treatments and potentially reducing healthcare costs associated with empirical treatments.
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- 2024
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32. Green tea extract reduces viral proliferation and ROS production during Feline Herpesvirus type-1 (FHV-1) infection.
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Longobardi C, Damiano S, Ferrara G, Esposito R, Montagnaro S, Florio S, and Ciarcia R
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- Animals, Cats, Cell Line, Tea chemistry, Cat Diseases drug therapy, Cat Diseases virology, Camellia sinensis chemistry, Plant Extracts pharmacology, Reactive Oxygen Species metabolism, Varicellovirus drug effects, Virus Replication drug effects, Herpesviridae Infections drug therapy, Herpesviridae Infections veterinary, Herpesviridae Infections virology, Antiviral Agents pharmacology
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Background: Feline Herpesvirus type-1 (FHV-1) is a worldwide spread pathogen responsible for viral rhinotracheitis and conjunctivitis in cats that, in the most severe cases, can lead to death. Despite the availability of a variety of antiviral medications to treat this illness, mainly characterized by virostatic drugs that alter DNA replication, their use is often debated. Phytotherapeutic treatments are a little-explored field for FHV-1 infections and reactivations. In this scenario, natural compounds could provide several advantages, such as reduced side effects, less resistance and low toxicity. The purpose of this study was to explore the potential inhibitory effects of the green tea extract (GTE), consisting of 50% of polyphenols, on FHV-1 infection and reactive oxygen species (ROS) production., Results: Crandell-Reese feline kidney (CRFK) cells were treated with different doses of GTE (10-400 µg/mL) during the viral adsorption and throughout the following 24 h. The MTT and TCID
50 assays were performed to determine the cytotoxicity and the EC50 of the extract, determining the amounts of GTE used for the subsequent investigations. The western blot assay showed a drastic reduction in the expression of viral glycoproteins (i.e., gB and gI) after GTE treatment. GTE induced not only a suppression in viral proliferation but also in the phosphorylation of Akt protein, generally involved in viral entry. Moreover, the increase in cell proliferation observed in infected cells upon GTE addition was supported by enhanced expression of Bcl-2 and Bcl-xL anti-apoptotic proteins. Finally, GTE antioxidant activity was evaluated by dichloro-dihydro-fluorescein diacetate (DCFH-DA) and total antioxidant capacity (TAC) assays. The ROS burst observed during FHV-1 infection was mitigated after GTE treatment, leading to a reduction in the oxidative imbalance., Conclusions: Although further clinical trials are necessary, this study demonstrated that the GTE could potentially serve as natural inhibitor of FHV-1 proliferation, by reducing viral entry. Moreover, it is plausible that the extract could inhibit apoptosis by modulating the intrinsic pathway, thus affecting ROS production., (© 2024. The Author(s).)- Published
- 2024
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33. Involvement in cyberbullying events and empathy are related to emotional responses to simulated social pain tasks.
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Morese R, Fabris MA, Longobardi C, and Marengo D
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Introduction: This study aims to explore the relationship between cyberbullying involvement either as a perpetrator or a victim and emotional responses to virtual social exclusion and inclusion. Previous research has predominantly focused on the impacts of in-person bullying. Our study shifts this focus to the cyber realm., Methods: A total of 156 adolescents living in northern Italy were recruited ( M
age : 12.26; SD = 0.87; 43% female). After completing measures of empathy and involvement in cyberbullying, adolescents participated in the cyberball tasks. Latent profile analysis was used to identify subgroups., Results: We found three groups: Class 3, reporting negative responses to the social exclusion tasks and positive responses to the social inclusion tasks; Class 1, reporting neutral emotional responses to social inclusion and negative emotional responses to social exclusion; and Class 2, showing neutral responses to 'social exclusion' tasks and strongly positive responses to 'social inclusion' tasks. Linear regression revealed that cyberbullies report a typical emotional response to exclusion and inclusion tasks (Class 3), whereas cybervictims are more likely to report negative responses to both exclusion and inclusion events (Class 1). High levels of empathy are associated with the manifestation of a typical emotional response (Class 3), in contrast to an impaired emotional response characterized by neutral or positive responses to conditions of 'social exclusion' and positive responses to conditions of 'social inclusion' (Class 2)., Conclusion: Results underscore the complex interplay between cyberbullying roles and emotional responses to virtual social experiences. Theoretical implications and limitations of the research are discussed., Competing Interests: All authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (© The Author(s) 2024.)- Published
- 2024
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34. Red Orange and Lemon Extract Ameliorates the Renal Oxidative Stress and Inflammation Induced by Ochratoxin A through the Modulation of Nrf2.
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Longobardi C, Damiano S, Fabroni S, Montagnaro S, Russo V, Vaccaro E, Giordano A, Florio S, and Ciarcia R
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- Animals, Rats, Oxidative Stress, Antioxidants, Inflammation, Kidney, NF-E2-Related Factor 2, Anthocyanins, Ochratoxins
- Abstract
Background: The presence of ochratoxin A (OTA) in food and feed is a public health concern. OTA intoxication is caused by several mechanisms, one of which consists of the alteration of the antioxidant activity of the cell due to the oxidative stress (OS). In this context, the use of natural antioxidant substances could be a potential biological decontamination method of mitigating the negative outcomes induced by OTA., Methods: we aimed to investigate how a red orange and lemon extract (RLE), rich in anthocyanins, would affect OTA-treated rats. The current work sought to clarify the renal protective efficacy of RLE in an OTA-treated rat model (RLE (90 mg/kg b.w.); OTA (0.5 mg/kg b.w.)) by investigating, thorough Western blot analysis, the involvement of the Nuclear factor erythroid 2-related factor 2 (Nrf2) pathway. The OS parameters and inflammatory status were evaluated by spectrophotometry. The inflammatory infiltrates in the kidney were evaluated by immunohistochemical assays., Results and Conclusion: Our findings showed a significant increase in oxidative and inflammatory parameters after OTA exposure, while the OTA + RLE co-treatment counteracted both the inflammatory and OS damage through the modulation of the Nrf2 pathway.
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- 2024
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35. Correction: Fabris et al. Exploring Multivariate Profiles of Psychological Distress and Empathy in Early Adolescent Victims, Bullies, and Bystanders Involved in Cyberbullying Episodes. Int. J. Environ. Res. Public Health 2022, 19 , 9871.
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Fabris MA, Longobardi C, Morese R, and Marengo D
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In the published publication [...].
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- 2024
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36. Protective Effects of a Red Orange and Lemon Extract (RLE) on the Hepatotoxicity Induced by Ochratoxin A in Rats.
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Longobardi C, Damiano S, Vaccaro E, Ballistreri G, Restucci B, Paciello O, Florio S, and Ciarcia R
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Ochratoxin A (OTA) is a highly potent mycotoxin that contaminates many kinds of food and feed sources. Its significant impact on human health and animal productivity makes it a topic of particular concern. The role of specific bioactive compounds used as dietary antioxidants is believed to be substantial due to their capacity to act as free radical scavengers. Because of the well-known oxidative stress induced by OTA, the primary objective of this work was to evaluate the antioxidant effects of a standardized powder extract recovered from citrus processing waste, red orange and lemon extract (RLE), on liver damage induced by OTA in a rat model. This study aimed to examine the impact of oral administration of RLE (90 mg/kg b.w.) on hepatic function and oxidative balance in Sprague-Dawley rats ( n = 6/group) treated with OTA (0.5 mg/kg b.w.) over a period of 14 days. The administration of OTA alone resulted in both biochemical changes and an imbalance in redox status in the liver. However, the use of RLE alleviated the activity of antioxidant enzymes and dramatically decreased the serum levels of ALT (alanine aminotransferase), AST (aspartate aminotransferase), and ALP (alkaline phosphatase), providing evidence of its protective benefits. Based on the findings from liver histology tests, the administration of RLE resulted in mitigation of lymphoplasmacytic inflammation, steatosis, and necrosis in the OTA group. These results indicate that the novel phytoextract RLE holds potential for application in the field of nutraceuticals.
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- 2024
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37. Apoptosis is mediated by FeHV-1 through the intrinsic pathway and interacts with the autophagic process.
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Ferrara G, Longobardi C, Sgadari MF, Restucci B, Iovane G, Ciarcia R, Pagnini U, and Montagnaro S
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- Cats, Animals, Caspase 3, Apoptosis Regulatory Proteins, NF-kappa B metabolism, Autophagy, Apoptosis, Caspases metabolism
- Abstract
Background: Although FeHV-1 is a primary feline pathogen, little is known about its interactions with host cells. Its relationship with several cellular pathways has recently been described, whereas its interplay with the apoptotic process, unlike other herpesviruses, has not yet been clarified. The aim of this work was to evaluate whether FeHV-1 induces apoptosis in its permissive cells, as well as the pathway involved and the effects of induction and inhibition of apoptosis on viral replication., Methods: Monolayers of CRFK cells were infected at different times with different viral doses. A cytofluorimetric approach allowed the quantification of cells in early and late apoptosis. All infections and related controls were also subjected to Western blot analysis to assess the expression of apoptotic markers (caspase 3-8-9, Bcl-2, Bcl-xL, NF-κB). An inhibitor (Z-VAD-FMK) and an inducer (ionomycin) were used to evaluate the role of apoptosis in viral replication. Finally, the expression of autophagy markers during the apoptosis inhibition/induction and the expression of apoptosis markers during autophagy inhibition/induction were evaluated to highlight any crosstalk between the two pathways., Results: FeHV-1 triggered apoptosis in a time- and dose-dependent manner. Caspase 3 cleavage was evident 48 h after infection, indicating the completeness of the process at this stage. While caspase 8 was not involved, caspase 9 cleavage started 24 h post-infection. The expression of other mitochondrial damage markers also changed, suggesting that apoptosis was induced via the intrinsic pathway. NF- κB was up-regulated at 12 h, followed by a gradual decrease in levels up to 72 h. The effects of apoptosis inhibitors and inducers on viral replication and autophagy were also investigated. Inhibition of caspases resulted in an increase in viral glycoprotein expression, higher titers, and enhanced autophagy, whereas induction of apoptosis resulted in a decrease in viral protein expression, lower viral titer, and attenuated autophagy. On the other hand, the induction of autophagy reduced the cleavage of caspase 3., Conclusions: In this study, we established how FeHV-1 induces the apoptotic process, contributing to the understanding of the relationship between FeHV-1 and this pathway., (© 2023. The Author(s).)
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- 2023
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38. Traditional Cultures versus Next Generation Sequencing for Suspected Orthopedic Infection: Experience Gained from a Reference Centre.
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Rimoldi SG, Brioschi D, Curreli D, Salari F, Pagani C, Tamoni A, Longobardi C, Bosari R, Rizzo A, Landonio S, Coen M, Passerini M, Gismondo MR, Gori A, and Manzotti A
- Abstract
(Background) The diagnosis and the antimicrobial treatment of orthopedic infection are challenging, especially in cases with culture-negative results. New molecular methods, such as next-generation sequencing (NGS), promise to overcome some limitations of the standard culture, such as the detection of difficult-to-grow bacteria. However, data are scarce regarding the impact of molecular techniques in real-life scenarios. (Methods) We included cases of suspected orthopedic infection treated with surgery from May 2021 to September 2023. We combined traditional cultures with NGS. For NGS, we performed a metagenomic analysis of ribosomal 16s, and we queried dedicated taxonomic libraries to identify the species. To avoid false positive results, we set a cut-off of 1000 counts of the percentage of frequency of reads. (Results) We included 49 patients in our study. Our results show the presence of bacteria in 36/49 (73%) and 29/49 (59%) cases studied with NGS and traditional cultures, respectively. The concordance rate was 61%. Among the 19/49 discordant cases, in 11/19 cases, cultures were negative and NGS positive; in 4/19, cultures were positive and NGS negative; and in the remaining 4/19, different species were detected by traditional cultures and NGS. (Conclusions) Difficult-to-grow microorganisms, such as slow-growing anaerobic bacteria, were better detected by NGS compared to traditional culture in our study. However, more data to distinguish between true pathogens and contaminants are needed. NGS can be an additional tool to be used for the diagnosis of orthopedic infections and the choice of appropriate antimicrobial therapy.
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- 2023
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39. Oxidative Status and Histological Evaluation of Wild Boars' Tissues Positive for Zearalenone Contamination in the Campania Region, Southern Italy.
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Damiano S, Longobardi C, Ferrara G, Piscopo N, Riccio L, Russo V, Meucci V, De Marchi L, Esposito L, Florio S, and Ciarcia R
- Abstract
Zearalenone (ZEN) is a mycotoxin produced by fungi belonging to the genera Fusarium spp. and commonly found in feed and food. It is frequently related to reproductive disorders in farm animals and, occasionally, to hyperestrogenic syndromes in humans. Nowadays, knowledge about ZEN effects on wild boars ( Sus scrofa ) is extremely scarce, despite the fact that they represent one of the most hunted game species in Italy. The aim of this study was to investigate how ZEN affects the liver, kidney, and muscle oxidative status and morphology of wild boars hunted in various locations throughout the province of Avellino, Campania Region, Southern Italy, during the 2021-2022 hunting season. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities, as well as the malondialdehyde (MDA) levels, were assessed by colorimetric assays; tissue morphology was evaluated by hematoxylin-eosin and Masson's stains. Our data showed that ZEN contamination might result in oxidative stress (OS) and some histopathological alterations in wild boars' livers and kidneys rather than in muscles, emphasizing the importance of developing a wildlife monitoring and management strategy for dealing not only with the problem of ZEN but the surveillance of mycotoxins in general.
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- 2023
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40. Editorial: Children's drawings: evidence-based research and practice.
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Fabris MA, Lange-Küttner C, Shiakou M, and Longobardi C
- Abstract
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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- 2023
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41. Autophagy up-regulation upon FeHV-1 infection on permissive cells.
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Ferrara G, Sgadari M, Longobardi C, Iovane G, Pagnini U, and Montagnaro S
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FeHV-1 is a member of the Herpesviridae family that is distributed worldwide and causes feline viral rhinotracheitis (FVR). Since its relationship with the autophagic process has not yet been elucidated, the aim of this work was to evaluate the autophagy mediated by FeHV-1 and to determine its proviral or antiviral role. Our data showed that autophagy is induced by FeHV-1 in a viral dose and time-dependent manner. Phenotypic changes in LC3/p62 axis (increase of LC3-II and degradation of p62) were detected from 12 h post infection using western blot and immuno-fluorescence assays. In a second step, by using late autophagy inhibitors and inducers, the possible proviral role of autophagy during FeHV-1 infection was investigating by assessing the effects of each chemical in terms of viral yield, cytotoxic effects, and expression of viral glycoproteins. Our findings suggest that late-stage autophagy inhibitors (bafilomycin and chloroquine) have a negative impact on viral replication. Interestingly, we observed an accumulation of gB, a viral protein, when cells were pretreated with bafilomycin, whereas the opposite effect was observed when an autophagy inducer was used. The importance of autophagy during FeHV-1 infection was further supported by the results obtained with ATG5 siRNA. In summary, this study demonstrates FeHV-1-mediated autophagy induction, its proviral role, and the negative impact of late autophagy inhibitors on viral replication., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Ferrara, Sgadari, Longobardi, Iovane, Pagnini and Montagnaro.)
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- 2023
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42. Investigating the effects of autonomy-supportive parenting practices on Italian young adolescent students' motivation to defend victims of bullying: findings on the mediating roles of reactance, depression, anxiety, and stress.
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Iotti NO, Menin D, Longobardi C, and Jungert T
- Abstract
This study investigated the effect of autonomy-supportive parenting practices on young adolescents' self-reported motivation to defend victims of bullying, and the possible mediating effects of factors such as reactance, anxiety, depression, and stress., Methods: Data were collected from 578 Italian public school students ages 10-14 (M
age = 11.8 years, 52% boys), who completed a survey in their classroom. The survey included self-report measures of parental orientation, motivation to defend victims of bullying, reactance, anxiety, depression, and stress., Results: We found that autonomy-supportive parenting had a positive effect on autonomous motivation to defend, and that this effect was weakly mediated by reactance. Moreover, autonomy-supportive parenting had a negative effect on extrinsic motivation to defend, which was partially mediated by reactance. Reactance had a positive direct effect on extrinsic motivation to defend, but results also showed that anxiety, depression, and stress did not mediate the effect of autonomy-supportive parenting on motivation to defend. Additionally, autonomy-supportive parenting appeared to play a protective role, being associated with lower levels of reactance, anxiety, depression, and stress. Finally, gender differences were found in our sample, with extrinsic motivation to defend being more prevalent in boys, and autonomous motivation to defend being more prevalent in girls. Girls also reported higher levels of anxiety, depression, and stress, compared to boys., Conclusion: Our findings show that autonomy-supportive parenting practices play a significant role in fostering young adolescents' motivation to defend victims of bullying, and that they are also linked with lower feelings of reactance, anxiety, depression, and stress. We argue that interventions aimed at contrasting bullying and cyberbullying among youths should seek to involve parents more and promote the adoption of more autonomy-supportive parenting practices, due to their consistently proven beneficial effects., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Iotti, Menin, Longobardi and Jungert.)- Published
- 2023
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43. Modifications of the PI3K/Akt/mTOR axis during FeHV-1 infection in permissive cells.
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Ferrara G, Longobardi C, Damiano S, Ciarcia R, Pagnini U, and Montagnaro S
- Abstract
FeHV-1 is the causative agent of infectious rhinotracheitis in cats. The relationship between viral infection and the PI3K/Akt/mTOR pathway, as well as its function in crucial physiological processes like as autophagy, apoptosis or the IFN induction cascade is known for other varicelloviruses. However, there is no information on whether autophagy is activated during FeHV-1 infection nor on how this infection modifies PI3K/Akt/mTOR pathway. In this work, we aim to elucidate the involvement of this pathway during cytolytic infection by FeHV-1 in permissive cell lines. Using a phenotypic approach, the expression of proteins involved in the PI3K/Akt/mTOR pathway was examined by Western blot analysis. The findings demonstrated the lack of modifications in relation to viral dose (except for phospho-mTOR), whereas there were changes in the expression of several markers in relation to time as well as a mismatch in the time of activation of this axis. These results suggest that FeHV-1 may interact independently with different autophagic signaling pathways. In addition, we found an early phosphorylation of Akt, approximately 3 h after infection, without a concomitant decrease in constitutive Akt. This result suggests a possible role for this axis in viral entry. In a second phase, the use of early autophagy inhibitors was examined for viral yield, cytotoxic effects, viral glycoprotein expression, and autophagy markers and resulted in inefficient inhibition of viral replication (12 h post-infection for LY294002 and 48 h post-infection for 3-methyladenine). The same markers were examined during Akt knockdown, and we observed no differences in viral replication. This result could be explained by the presence of a protein kinase in the FeHV-1 genome (encoded by the Us3 gene) that can phosphorylate various Akt substrates as an Akt surrogate, as has already been demonstrated in genetically related viruses (HSV-1, PRV, etc.). For the same reasons, the use of LY294002 at the beginning of infection did not affect FeHV-1-mediated Akt phosphorylation. Our findings highlight changes in the PI3K/Akt/mTOR pathway during FeHV-1 infection, although further research is needed to understand the importance of these changes and how they affect cellular processes and viral propagation., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Ferrara, Longobardi, Damiano, Ciarcia, Pagnini and Montagnaro.)
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- 2023
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44. Subtype-specific kinase dependency regulates growth and metastasis of poor-prognosis mesenchymal colorectal cancer.
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Buikhuisen JY, Gomez Barila PM, Cameron K, Suijkerbuijk SJE, Lieftink C, di Franco S, Krotenberg Garcia A, Uceda Castro R, Lenos KJ, Nijman LE, Torang A, Longobardi C, de Jong JH, Dekker D, Stassi G, Vermeulen L, Beijersbergen RL, van Rheenen J, Huveneers S, and Medema JP
- Subjects
- Humans, Actin Cytoskeleton, Carcinogenesis, Cell Line, Sarcoma, Colorectal Neoplasms
- Abstract
Background: Colorectal cancer (CRC) can be divided into four consensus molecular subtypes (CMS), each with distinct biological features. CMS4 is associated with epithelial-mesenchymal transition and stromal infiltration (Guinney et al., Nat Med 21:1350-6, 2015; Linnekamp et al., Cell Death Differ 25:616-33, 2018), whereas clinically it is characterized by lower responses to adjuvant therapy, higher incidence of metastatic spreading and hence dismal prognosis (Buikhuisen et al., Oncogenesis 9:66, 2020)., Methods: To understand the biology of the mesenchymal subtype and unveil specific vulnerabilities, a large CRISPR-Cas9 drop-out screen was performed on 14 subtyped CRC cell lines to uncover essential kinases in all CMSs. Dependency of CMS4 cells on p21-activated kinase 2 (PAK2) was validated in independent 2D and 3D in vitro cultures and in vivo models assessing primary and metastatic outgrowth in liver and peritoneum. TIRF microscopy was used to uncover actin cytoskeleton dynamics and focal adhesion localization upon PAK2 loss. Subsequent functional assays were performed to determine altered growth and invasion patterns., Results: PAK2 was identified as a key kinase uniquely required for growth of the mesenchymal subtype CMS4, both in vitro and in vivo. PAK2 plays an important role in cellular attachment and cytoskeletal rearrangements (Coniglio et al., Mol Cell Biol 28:4162-72, 2008; Grebenova et al., Sci Rep 9:17171, 2019). In agreement, deletion or inhibition of PAK2 impaired actin cytoskeleton dynamics in CMS4 cells and, as a consequence, significantly reduced invasive capacity, while it was dispensable for CMS2 cells. Clinical relevance of these findings was supported by the observation that deletion of PAK2 from CMS4 cells prevented metastatic spreading in vivo. Moreover, growth in a model for peritoneal metastasis was hampered when CMS4 tumor cells were deficient for PAK2., Conclusion: Our data reveal a unique dependency of mesenchymal CRC and provide a rationale for PAK2 inhibition to target this aggressive subgroup of colorectal cancer., (© 2023. The Author(s).)
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- 2023
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45. Zearalenone (ZEN) and Its Metabolite Levels in Tissues of Wild Boar ( Sus scrofa ) from Southern Italy: A Pilot Study.
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Longobardi C, Damiano S, Ferrara G, Montagnaro S, Meucci V, Intorre L, Bacci S, Esposito L, Piscopo N, Rubino A, Raffaele A, Florio S, and Ciarcia R
- Subjects
- Humans, Animals, Swine, Pilot Projects, Sus scrofa metabolism, Zearalenone toxicity, Mycotoxins toxicity, Estrogens, Non-Steroidal toxicity
- Abstract
Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin produced by the fungi of the Fusarium genera, and is a contaminant of cereals and plant products. ZEN and its metabolites are considered endocrine disruptors, and could have various toxic effects on animals and humans. In recent years, there has been a significant demographic increase in wild boar ( Sus scrofa ) in many mountainous and hilly areas of Italy, including the Campania region, mainly due to global climate change. The wild boar can be defined as a generalist and omnivorous species capable of varying its diet; therefore, it can play a role as an environmental bioindicator towards contaminants such as mycotoxins. This study was conducted to evaluate, for the first time, the concentrations of ZEN and its metabolites in the liver, kidney, and muscle of 82 wild boars shot in their habitat by hunters with hunting permits in different localities of Avellino province (Campania region, Southern Italy) from 2021 to 2022. The samples were collected and analyzed with an SPE clean-up and high-pressure liquid chromatography method with fluorescence detection. The results indicated that ZEN and α-Zearalenol were present in most of the samples, suggesting that a plan to monitor these mycoestrogens is essential to achieve the goals of "One Health".
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- 2023
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46. Relational Victimization and Video Game Addiction among Female College Students during COVID-19 Pandemic: The Roles of Social Anxiety and Parasocial Relationship.
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Niu G, Jin S, Xu F, Lin S, Zhou Z, and Longobardi C
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- Humans, Female, Pandemics, Students, Technology Addiction, Anxiety epidemiology, COVID-19 epidemiology, Crime Victims
- Abstract
Video game addiction, a common behavioral problem among college students, has been more prominent during the COVID-19 pandemic; at the same time, females' video game usage has also attracted considerable research attention. Against this background and under the perspective of social interaction, this study aimed to examine the relationship between relational victimization and video game addiction among female college students, as well as its underlying mechanism-the mediating roles of social anxiety and parasocial relationships with virtual characters. Female college students ( N = 437) were recruited to complete a set of questionnaires voluntarily in June 2022. Through the mediating effect analysis, the results found that (1) relational victimization was positively associated with female college students' video game addiction; (2) social anxiety and parasocial relationships with virtual characters could independently mediate this relation; (3) social anxiety and parasocial relationships with virtual characters were also the serial mediators in this association. These findings not only expand previous studies by revealing the social motivation of video game usage and the underlying mechanism accounting for video game addiction, but also provide basis and guidance for the prevention and intervention of video game addiction in the current context of the COVID-19 pandemic.
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- 2022
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47. Loss of primary cilia promotes inflammation and carcinogenesis.
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Paul C, Tang R, Longobardi C, Lattanzio R, Eguether T, Turali H, Bremond J, Maurizy C, Gabola M, Poupeau S, Turtoi A, Denicolai E, Cufaro MC, Svrcek M, Seksik P, Castronovo V, Delvenne P, de Laurenzi V, Da Costa Q, Bertucci F, Lemmers B, Pieragostino D, Mamessier E, Janke C, Pinet V, and Hahne M
- Subjects
- Mice, Animals, Cilia, Interleukin-6 genetics
- Abstract
Primary cilia (PC) are important signaling hubs, and we here explored their role in colonic pathology. In the colon, PC are mostly present on fibroblasts, and exposure of mice to either chemically induced colitis-associated colon carcinogenesis (CAC) or dextran sodium sulfate (DSS)-induced acute colitis decreases PC numbers. We generated conditional knockout mice with reduced numbers of PC on colonic fibroblasts. These mice show increased susceptibility to CAC, as well as DSS-induced colitis. Secretome and immunohistochemical analyses of DSS-treated mice display an elevated production of the proinflammatory cytokine IL-6 in PC-deficient colons. An inflammatory environment diminishes PC presence in primary fibroblast cultures, which is triggered by IL-6 as identified by RNA-seq analysis together with blocking experiments. These findings suggest an activation loop between IL-6 production and PC loss. An analysis of PC presence on biopsies of patients with ulcerative colitis or colorectal cancer (CRC) reveals decreased numbers of PC on colonic fibroblasts in pathological compared with surrounding normal tissue. Taken together, we provide evidence that a decrease in colonic PC numbers promotes colitis and CRC., (© 2022 The Authors. Published under the terms of the CC BY NC ND 4.0 license.)
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- 2022
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48. The Microalga Skeletonema marinoi Induces Apoptosis and DNA Damage in K562 Cell Line by Modulating NADPH Oxidase.
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Ciarcia R, Longobardi C, Ferrara G, Montagnaro S, Andretta E, Pagnini F, Florio S, Maruccio L, Lauritano C, and Damiano S
- Subjects
- Humans, Apoptosis, NADPH Oxidases metabolism, K562 Cells, Reactive Oxygen Species metabolism, DNA Damage, Proto-Oncogene Proteins c-bcl-2 genetics, Leukemia, Myelogenous, Chronic, BCR-ABL Positive genetics, Diatoms metabolism
- Abstract
Chronic myeloid leukemia (CML) is a myeloproliferative disease that activates multiple signaling pathways, causing cells to produce higher levels of reactive oxygen species (ROS). Nicotinamide adenine dinucleotide phosphate (NADPH) oxidases (NOXs) are a major generator of ROS in leukemia, and marine natural products have shown promising activities for the treatment of hematopoietic malignancies. In the present study, we investigated the effect of the marine microalga Skeletonema marinoi (S.M.), a ubiquitous diatom that forms massive blooms in the oceans, on the human leukemia cell line K562. The effects of S.M. extract on cell viability, production of ROS, nitric oxide (NO), and apoptosis were examined. In this preliminary work, S.M. was able to decrease cell viability (p < 0.05) and increase apoptosis levels (p < 0.05) in K562 cells after 48 h of treatment. In addition, the levels of NOX, NO, and malondialdehyde (MDA) were reduced in K562-treated cells (p < 0.05), whereas the levels of SOD, CAT, and GPx increased during treatment (p < 0.05). Finally, analyzing Bax and Bcl-2 expression, we found a significant increase in the proapoptotic protein Bax and a sustained decrease in the antiapoptotic protein Bcl-2 (p < 0.05) in the K562-treated cells.
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- 2022
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49. Author Correction: The HSP90/R2TP assembly chaperone promotes cell proliferation in the intestinal epithelium.
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Maurizy C, Abeza C, Lemmers B, Gabola M, Longobardi C, Pinet V, Ferrand M, Paul C, Bremond J, Langa F, Gerbe F, Jay P, Verheggen C, Tinari N, Helmlinger D, Lattanzio R, Bertrand E, Hahne M, and Pradet-Balade B
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- 2022
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50. The representation of bullying in Italian primary school children: A mixed-method study comparing drawing and interview data and their association with self-report involvement in bullying events.
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Marengo D, Settanni M, Longobardi C, and Fabris MA
- Abstract
Bullying continues to be a social issue affecting millions of students of all ages worldwide. Research on bullying seems to be dominated by quantitative research approaches employed standardized categories and measures, ultimately limiting our knowledge about children's own view on bullying. Our research follows another direction, aiming to explore the representation of bullying in a sample of Italian primary school children by using and comparing the functioning of two qualitative research instruments: interviews, and children's drawings. In addition, aided by quantitative analyses, we aimed to investigate whether students' involvement in different bullying roles (as bullies, victims, or defenders), as measured by self-assessment, correlated with different characteristics of the representation of bullying emerging from children's drawings and interviews. We recruited a convenient sample of 640 primary school students (mean age = 9.44; SD = 0.67), 53.3% of whom were male. The results showed that all forms of bullying, i.e., physical, verbal, and social bullying, could be identified in interview and drawing data, although references to all types of bullying were more frequent in interview data. In terms of bullying criteria, the presence of a power imbalance between the bully and the victim was most frequently detected in both the interview data and the drawing data, while repetition was more easily detected in the interview data. The interview data showed that sadness was the most frequently reported victim emotions, followed by fear, anger, and lack of emotion. The drawing data showed a similar pattern, although victims were more frequently described as lacking emotions compared to the interview data. In both interview and drawing data, age and female gender were positively associated with references to verbal bullying, and negatively associated with references to physical bullying. Additionally, bully/victim children were more likely than uninvolved children to depict physical bullying in the drawings, while this association was not detected in interview data. In summary, our study shows that, compared with drawings, interviews tend to provide a more comprehensive view of children's own representation of bullying, while drawing data tend to show stronger connections with children's current personal experiences of bullying., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The reviewer SB declared a past collaboration with the authors, CL and MF to the handling editor., (Copyright © 2022 Marengo, Settanni, Longobardi and Fabris.)
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- 2022
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