Back to Search
Start Over
Evaluation of 16S-Based Metagenomic NGS as Diagnostic Tool in Different Types of Culture-Negative Infections.
- Source :
-
Pathogens (Basel, Switzerland) [Pathogens] 2024 Aug 30; Vol. 13 (9). Date of Electronic Publication: 2024 Aug 30. - Publication Year :
- 2024
-
Abstract
- Bacterial infections pose significant global health challenges, often underestimated due to difficulties in accurate diagnosis, especially when culture-based diagnostics fail. This study assesses the effectiveness of 16S-based metagenomic next generation sequencing (NGS) for identifying pathogens in culture-negative clinical samples across various medical settings. Overall, 48% of samples were collected from orthopedics, 15% from neurosurgery, and 12% in cardiac surgery, among others. The detection rate of monomicrobial infections was 68.6%, and 5.7% for polymicrobial infections. In addition, NGS detected bacteria in all samples from the lungs, head and neck, and eye specimens. Cutibacterium acnes (11%, 12/105) was the most frequent microorganism, followed by Staphylococcus epidermidis (10.4%, 11/105), and Staphylococcus aureus (9.5%, 10/105). In conclusion, 16S-targeted metagenomic sequencing enhances pathogen detection capabilities, particularly in instances where traditional cultures fail. By the combination of NGS and bacterial cultures, microbiologists might provide a more accurate diagnosis, guiding more effective treatments and potentially reducing healthcare costs associated with empirical treatments.
Details
- Language :
- English
- ISSN :
- 2076-0817
- Volume :
- 13
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Pathogens (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 39338934
- Full Text :
- https://doi.org/10.3390/pathogens13090743