20 results on '"Lafaye C"'
Search Results
2. An improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging
- Author
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Yu, D, Gustafson, WCL, Han, C, Lafaye, C, Noirclerc-Savoye, M, Ge, WP, Thayer, DA, Huang, H, Kornberg, TB, Royant, A, Jan, LYE, Jan, YNU, Weiss, WA, and Shu, X
- Abstract
Infrared fluorescent proteins (IFPs) are ideal for in vivo imaging, and monomeric versions of these proteins can be advantageous as protein tags or for sensor development. In contrast to GFP, which requires only molecular oxygen for chromophore maturation, phytochrome-derived IFPs incorporate biliverdin (BV) as the chromophore. However, BV varies in concentration in different cells and organisms. Here we engineered cells to express the haeme oxygenase responsible for BV biosynthesis and a brighter monomeric IFP mutant (IFP2.0). Together, these tools improve the imaging capabilities of IFP2.0 compared with monomeric IFP1.4 and dimeric iRFP. By targeting IFP2.0 to the plasma membrane, we demonstrate robust labelling of neuronal processes in Drosophila larvae. We also show that this strategy improves the sensitivity when imaging brain tumours in whole mice. Our work shows promise in the application of IFPs for protein labelling and in vivo imaging.
- Published
- 2014
3. La sueur comme indicateur de la santé [Sweat as an indicator of health]
- Author
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Saubade, M., Norrenberg, S., Besson, C., Demuru, S., Briand, D., Paul, B., Gremeaux, V., and Lafaye, C.
- Subjects
Exercise ,Hot Temperature ,Humans ,Skin ,Sweat ,Sweating - Abstract
Sweat is a body fluid produced by the sweat glands and is mainly composed of water. Sweat has various functions, the two main ones being the evacuation of heat produced by the body, especially during exercise, and the maintenance of skin homeostasis. Its production is highly variable and depends on many individual and environmental factors. Various diseases or conditions affect its proper functioning. This article presents an overview of the characteristics, the main health issues, and the current and potential applications related to sweat.
- Published
- 2021
4. Tailing miniSOG: structural bases of the complex photophysics of a flavin-binding singlet oxygen photosensitizing protein
- Author
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Torra J., Lafaye C., Signor L., Aumonier S., Flors C., Shu X., Nonell S., Gotthard G., Royant A. and We thank Rubén Ruiz-González, Marjolaine Noirclerc-Savoye and David von Stetten for their contribution at an early stage of the project. The ESRF is acknowledged for access to beamlines and facilities for molecular biology via its in-house research program. AR acknowledges funding from the French Agence Nationale de la Recherche (project SOxygen, ANR-11-JSV5-0009 and project CrystalBall, ANR-14-CE06-0005-02), from the Spanish Ministerio de Economía y Competitividad (CTQ2016-78454-C2-1-R, MAT2015-66605-P and SEV-2016-0686) and the Fundació la Marató de TV3 (grant No. 20133133). This work used the platforms of the Grenoble Instruct-ERIC Center (ISBG: UMS 3518 CNRS-CEA-UGA-EMBL) with support from FRISBI (ANR-10-INBS-05-02) and GRAL (ANR-10-LABX-49-01) within the Grenoble Partnership for Structural Biology (PSB).
- Published
- 2019
5. Annonce à l'enfant et à l'adolescent de son statut VIH en Afrique francophone centrale et de l'Ouest
- Author
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Dahourou, D.L., Masson, D., Aka-Dago-Akribi, H., Gauthier-Lafaye, C., Cacou, C., Raynaud, J.P., Moh, C., Bouah, B., Sturm, G., Oga, M., Msellati, Philippe, Leroy, V., and Groupe Atelier Annonce Adolescents Afrique
- Abstract
Nous rapportons les attitudes et pratiques des soignants en Afrique francophone concernant l'annonce du statut VIH aux adolescents, et les témoignages de jeunes vivant avec le VIH (jvVIH). Lors d'un atelier de trois jours à Abidjan, Côte d'Ivoire, en novembre 2016, les soignants (médecins, psychologues, travailleurs sociaux) de 19 sites de prise en charge pédiatrique du VIH ont partagé leurs pratiques et difficultés et 4 jvVIH leur vécu de l'annonce. Au total, 35 participants de 8 pays d'Afrique de l'Ouest/centrale (Bénin, Burkina Faso, Côte d'Ivoire, Cameroun, Mali, République démocratique du Congo, Sénégal, Togo) ont contribué : 14 médecins, 8 psychologues, 6 conseillers, 3 travailleurs sociaux. L'expérience des centres était variable, mais l'âge à l'annonce restait tardif : 34 % des 1 296 adolescents âgés entre 10 et 12 ans connaissaient leur statut. L'âge médian à l'annonce était de 13 ans (étendue : 11-15 ans). La pratique de l'annonce s'avérait complexe, en raison de multiples facteurs (crainte des parents de la rupture du secret, manque de communication entre professionnels). L'annonce individuelle était la pratique majoritairement adoptée. Quatre centres pratiquaient une annonce en séances de groupe pour faciliter le soutien en miroir, et un avait recours à l'appui de pairs-adolescents. Les jvVIH ont plaidé pour une annonce plus précoce, dès 10 ans. En Afrique de l'Ouest/centrale francophone, le processus de l'annonce reste complexe pour parents et soignants, et l'annonce trop tardive. L'élaboration d'un guide de bonnes pratiques de l'annonce du VIH, adapté aux contextes socio-culturels devrait permettre d'améliorer ce processus.
- Published
- 2019
6. Tailing miniSOG: structural bases of the complex photophysics of a flavin-binding singlet oxygen photosensitizing protein
- Author
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Torra J., Lafaye C., Signor L., Aumonier S., Flors, Cristina, Shu X., Nonell S., Gotthard G., Royant A., Torra J., Lafaye C., Signor L., Aumonier S., Flors, Cristina, Shu X., Nonell S., Gotthard G., and Royant A.
- Published
- 2019
7. Assessing the performance of a robust multiparametric wearable patch integrating silicon-based sensors for real-time continuous monitoring of sweat biomarkers.
- Author
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Rovira M, Lafaye C, Demuru S, Kunnel BP, Aymerich J, Cuenca J, Serra-Graells F, Margarit-Taulé JM, Haque R, Saubade M, Fernández-Sánchez C, and Jimenez-Jorquera C
- Subjects
- Humans, Equipment Design, Sodium analysis, Potassium analysis, Hydrogen-Ion Concentration, Monitoring, Physiologic instrumentation, Wearable Electronic Devices, Sweat chemistry, Biosensing Techniques instrumentation, Silicon chemistry, Biomarkers analysis, Transistors, Electronic
- Abstract
The development of wearable devices for sweat analysis has experienced significant growth in the last two decades, being the main focus the monitoring of athletes health during workouts. One of the main challenges of these approaches has been to attain the continuous monitoring of sweat for time periods over 1 h. This is the main challenge addressed in this work by designing an analytical platform that combines the high performance of potentiometric sensors and a fluidic structure made of a plastic fabric into a multiplexed wearable device. The platform comprises Ion-Sensitive Field-Effect Transistors (ISFETs) manufactured on silicon, a tailor-made solid-state reference electrode, and a temperature sensor integrated into a patch-like polymeric substrate, together with the component that easily collects and drives samples under continuous capillary flow to the sensor areas. ISFET sensors for measuring pH, sodium, and potassium ions were fully characterized in artificial sweat solutions, providing reproducible and stable responses. Then, the real-time and continuous monitoring of the biomarkers in sweat with the wearable platform was assessed by comparing the ISFETs responses recorded during an 85-min continuous exercise session with the concentration values measured using commercial Ion-Selective Electrodes (ISEs) in samples collected at certain times during the session. The developed sensing platform enables the continuous monitoring of biomarkers and facilitates the study of the effects of various real working conditions, such as cycling power and skin temperature, on the target biomarker concentration levels., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
8. Identification and Characterization of an Exonic Duplication in PALB2 in a Man with Synchronous Breast and Prostate Cancer.
- Author
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Bouras A, Lafaye C, Leone M, Kherraf ZE, Martin-Denavit T, Fert-Ferrer S, Calender A, and Boutry-Kryza N
- Subjects
- Alternative Splicing genetics, Alu Elements genetics, Base Sequence, DNA, Neoplasm genetics, Frameshift Mutation genetics, Humans, Male, Middle Aged, Breast Neoplasms, Male genetics, Exons genetics, Fanconi Anemia Complementation Group N Protein genetics, Gene Duplication, Genetic Predisposition to Disease, Neoplasms, Multiple Primary genetics, Prostatic Neoplasms genetics
- Abstract
PALB2 (partner and localizer of BRCA2 ), as indicated by its name, is a BRCA2 -interacting protein that plays an important role in homologous recombination (HR) and DNA double-strand break (DSB) repair. While pathogenic variants of PALB2 have been well proven to confer an increased risk of breast cancer, data on its involvement in prostate cancer (PrC) have not been clearly demonstrated. We investigated, using targeted next generation sequencing (NGS), a 59-year-old Caucasian man who developed synchronous breast and prostate cancers. This genetic investigation allowed to identify an intragenic germline heterozygous duplication in PALB2 , implicating intronic repetitive sequences spanning exon 11. This variant was confirmed by multiplex ligation probe amplification (MLPA), and genomic breakpoints have been identified and characterized at the nucleotide level (c.3114-811_3202-1756dup) using an approach based on walking PCR, long range PCR, and Sanger sequencing. RT-PCR using mRNA extracted from lymphocytes and followed by Sanger sequencing revealed a tandem duplication r.3114_3201dup; p.(Gly1068Glufs * 14). This duplication results in the synthesis of a truncated, and most-likely, non-functional protein. These findings expand the phenotypic spectrum of PALB2 variants and may improve the yield of genetic diagnoses in this field.
- Published
- 2022
- Full Text
- View/download PDF
9. Dynamics of a family of cyan fluorescent proteins probed by incoherent neutron scattering.
- Author
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Golub M, Guillon V, Gotthard G, Zeller D, Martinez N, Seydel T, Koza MM, Lafaye C, Clavel D, von Stetten D, Royant A, and Peters J
- Subjects
- Green Fluorescent Proteins chemistry, Molecular Dynamics Simulation, Neutrons, Scattering, Radiation
- Abstract
Cyan fluorescent proteins (CFPs) are variants of green fluorescent proteins in which the central tyrosine of the chromophore has been replaced by a tryptophan. The increased bulk of the chromophore within a compact protein and the change in the positioning of atoms capable of hydrogen bonding have made it difficult to optimize their fluorescence properties, which took approximately 15 years between the availability of the first useable CFP, enhanced cyan fluorescent protein (ECFP), and that of a variant with almost perfect fluorescence efficiency, mTurquoise2. To understand the molecular bases of the progressive improvement in between these two CFPs, we have studied by incoherent neutron scattering the dynamics of five different variants exhibiting progressively increased fluorescence efficiency along the evolution pathway. Our results correlate well with the analysis of the previously determined X-ray crystallographic structures, which show an increase in flexibility between ECFP and the second variant, Cerulean, which is then hindered in the three later variants, SCFP3A (Super Cyan Fluorescent Protein 3A), mTurquoise and mTurquoise2. This confirms that increasing the rigidity of the direct environment of the fluorescent chromophore is not the sole parameter leading to brighter fluorescent proteins and that increased flexibility in some cases may be helpful.
- Published
- 2019
- Full Text
- View/download PDF
10. Tailing miniSOG: structural bases of the complex photophysics of a flavin-binding singlet oxygen photosensitizing protein.
- Author
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Torra J, Lafaye C, Signor L, Aumonier S, Flors C, Shu X, Nonell S, Gotthard G, and Royant A
- Subjects
- Arabidopsis Proteins chemistry, Arabidopsis Proteins ultrastructure, Biophysical Phenomena, Flavins chemistry, Flavins genetics, Light, Microscopy, Electron, Oxidation-Reduction, Oxygen metabolism, Photosensitivity Disorders, Photosensitizing Agents chemistry, Protein Binding genetics, Protein Engineering, Protein Serine-Threonine Kinases chemistry, Protein Serine-Threonine Kinases ultrastructure, Singlet Oxygen chemistry, Arabidopsis Proteins genetics, Photosensitizing Agents metabolism, Protein Conformation, Protein Serine-Threonine Kinases genetics, Singlet Oxygen metabolism
- Abstract
miniSOG is the first flavin-binding protein that has been developed with the specific aim of serving as a genetically-encodable light-induced source of singlet oxygen (
1 O2 ). We have determined its 1.17 Å resolution structure, which has allowed us to investigate its mechanism of photosensitization using an integrated approach combining spectroscopic and structural methods. Our results provide a structural framework to explain the ability of miniSOG to produce1 O2 as a competition between oxygen- and protein quenching of its triplet state. In addition, a third excited-state decay pathway has been identified that is pivotal for the performance of miniSOG as1 O2 photosensitizer, namely the photo-induced transformation of flavin mononucleotide (FMN) into lumichrome, which increases the accessibility of oxygen to the flavin FMN chromophore and makes protein quenching less favourable. The combination of the two effects explains the increase in the1 O2 quantum yield by one order of magnitude upon exposure to blue light. Besides, we have identified several surface electron-rich residues that are progressively photo-oxidized, further contributing to facilitate the production of1 O2 . Our results help reconcile the apparent poor level of1 O2 generation by miniSOG and its excellent performance in correlative light and electron microscopy experiments.- Published
- 2019
- Full Text
- View/download PDF
11. Sulfur denitrosylation by an engineered Trx-like DsbG enzyme identifies nucleophilic cysteine hydrogen bonds as key functional determinant.
- Author
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Lafaye C, Van Molle I, Tamu Dufe V, Wahni K, Boudier A, Leroy P, Collet JF, and Messens J
- Published
- 2017
- Full Text
- View/download PDF
12. Transition from paediatric to adult care of adolescents living with HIV in sub-Saharan Africa: challenges, youth-friendly models, and outcomes.
- Author
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Dahourou DL, Gautier-Lafaye C, Teasdale CA, Renner L, Yotebieng M, Desmonde S, Ayaya S, Davies MA, and Leroy V
- Subjects
- Adolescent, Africa South of the Sahara epidemiology, Africa, Eastern, Child, Female, Humans, Male, Models, Organizational, Prevalence, Social Stigma, Young Adult, HIV Infections epidemiology, HIV Infections therapy, Transition to Adult Care
- Abstract
Introduction: The number of adolescents with perinatally or behaviourally acquired HIV is increasing in low-income countries, and especially in sub-Saharan Africa where HIV prevalence and incidence are the highest. As they survive into adulthood in the era of antiretroviral therapy, there is a pressing need to transfer them from paediatric to adult care, known as the transition of care. We conducted a narrative review of recent evidence on their transition outcomes in Africa, highlighting the specific needs and challenges in these populations and settings, and the different models of care for transition., Areas Covered: We searched PubMed bibliographic database, HIV conference content, and grey literature from January 2000 to August 2016 with the following keywords: HIV infections AND (adolescents or youth) AND transition AND Africa. All qualitative and quantitative, experimental and observational studies including HIV-infected patients aged 10-24 years with information on transition were eligible., Results: Few data on transition outcomes for HIV-infected adolescents are available from Africa settings. Studies mainly from Southern and East Africa reported on the barriers to successful transition, highlighting several gaps. These included lack of adequate infrastructure, staff training and communication between paediatric and adult clinicians as well as the fear of stigma of adolescents and youth living with HIV. Most countries have no specific national guidelines on when to disclose HIV status or when and how to transition to adult care. Several models of care adapted to the adolescent transition question have been implemented in specific settings. These models include teen clinics, peer educators or the use of social media. However, regardless of the model, services are increasingly overburdened and have insufficient human resources. Furthermore, very high attrition has been observed among adolescents and youth compared to younger children or older adults. There is a need to identify sub-groups at higher risk of loss to follow-up for targeted care and peer support., Expert Commentary: Although the available HIV-related data on adolescent transition outcomes are limited, there is evidence of their increased vulnerability during this period. Standardized data gathering, analysis, and reporting systems specific to adolescent transition are essential to improve understanding and adolescent outcomes in Africa., Competing Interests: The authors have no competing interests to declare.
- Published
- 2017
- Full Text
- View/download PDF
13. Precision Optogenetic Tool for Selective Single- and Multiple-Cell Ablation in a Live Animal Model System.
- Author
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Makhijani K, To TL, Ruiz-González R, Lafaye C, Royant A, and Shu X
- Subjects
- Animals, Cell Engineering, HEK293 Cells, Humans, Larva cytology, Larva drug effects, Neurons metabolism, Photosensitizing Agents chemistry, Drosophila melanogaster cytology, Models, Animal, Neurons drug effects, Optogenetics, Photosensitizing Agents pharmacology, Singlet Oxygen metabolism
- Abstract
Cell ablation is a strategy to study cell lineage and function during development. Optogenetic methods are an important cell-ablation approach, and we have previously developed a mini singlet oxygen generator (miniSOG) tool that works in the living Caenorhabditis elegans. Here, we use directed evolution to generate miniSOG2, an improved tool for cell ablation via photogenerated reactive oxygen species. We apply miniSOG2 to a far more complex model animal system, Drosophila melanogaster, and demonstrate that it can be used to kill a single neuron in a Drosophila larva. In addition, miniSOG2 is able to photoablate a small group of cells in one of the larval wing imaginal discs, resulting in an adult with one incomplete and one normal wing. We expect miniSOG2 to be a useful optogenetic tool for precision cell ablation at a desired developmental time point in live animals, thus opening a new window into cell origin, fate and function, tissue regeneration, and developmental biology., (Published by Elsevier Ltd.)
- Published
- 2017
- Full Text
- View/download PDF
14. Sulfur Denitrosylation by an Engineered Trx-like DsbG Enzyme Identifies Nucleophilic Cysteine Hydrogen Bonds as Key Functional Determinant.
- Author
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Lafaye C, Van Molle I, Tamu Dufe V, Wahni K, Boudier A, Leroy P, Collet JF, and Messens J
- Subjects
- Binding Sites, Cysteine, Escherichia coli genetics, Escherichia coli metabolism, Escherichia coli Proteins chemistry, Escherichia coli Proteins genetics, Hydrogen Bonding, Hydrogen-Ion Concentration, Models, Molecular, Mutagenesis, Site-Directed, Nitrosation, Oxidoreductases chemistry, Oxidoreductases genetics, Periplasmic Proteins chemistry, Periplasmic Proteins genetics, Protein Engineering, Protein Stability, Recombinant Fusion Proteins chemistry, Recombinant Fusion Proteins genetics, Recombinant Fusion Proteins metabolism, S-Nitrosoglutathione metabolism, Sulfur metabolism, Thioredoxins chemistry, Thioredoxins genetics, Escherichia coli Proteins metabolism, Oxidoreductases metabolism, Periplasmic Proteins metabolism, Thioredoxins metabolism
- Abstract
Exposure of bacteria to NO results in the nitrosylation of cysteine thiols in proteins and low molecular weight thiols such as GSH. The cells possess enzymatic systems that catalyze the denitrosylation of these modified sulfurs. An important player in these systems is thioredoxin (Trx), a ubiquitous, cytoplasmic oxidoreductase that can denitrosylate proteins in vivo and S-nitrosoglutathione (GSNO) in vitro However, a periplasmic or extracellular denitrosylase has not been identified, raising the question of how extracytoplasmic proteins are repaired after nitrosative damage. In this study, we tested whether DsbG and DsbC, two Trx family proteins that function in reducing pathways in the Escherichia coli periplasm, also possess denitrosylating activity. Both DsbG and DsbC are poorly reactive toward GSNO. Moreover, DsbG is unable to denitrosylate its specific substrate protein, YbiS. Remarkably, by borrowing the CGPC active site of E. coli Trx-1 in combination with a T200M point mutation, we transformed DsbG into an enzyme highly reactive toward GSNO and YbiS. The pKa of the nucleophilic cysteine, as well as the redox and thermodynamic properties of the engineered DsbG are dramatically changed and become similar to those of E. coli Trx-1. X-ray structural insights suggest that this results from a loss of two direct hydrogen bonds to the nucleophilic cysteine sulfur in the DsbG mutant. Our results highlight the plasticity of the Trx structural fold and reveal that the subtle change of the number of hydrogen bonds in the active site of Trx-like proteins is the key factor that thermodynamically controls reactivity toward nitrosylated compounds., (© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.)
- Published
- 2016
- Full Text
- View/download PDF
15. Multidrug-resistant bacteria transmitted through high-density EEG in ICU.
- Author
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Weiss N, Faugeras F, Rohaut B, Leconte J, Lafeuille E, Brossier F, Bourmaleau J, Lefebvre S, Lafaye C, Quirins M, Valente M, Brisson H, Mayaux J, Rudler M, Bréchot N, Demeret S, Bolgert F, Robert J, and Naccache L
- Subjects
- Adult, Aged, Female, Humans, Male, Middle Aged, beta-Lactamases pharmacology, Bacterial Infections transmission, Drug Resistance, Microbial drug effects, Electroencephalography, Intensive Care Units
- Abstract
Purpose: Prevention of multidrug resistant (MDR) bacterial contamination remains a major challenge in ICUs. Many hospital outbreaks involving MDR transmitted through environmental contamination have been reported. Bedside high-density EEG allow for dynamic cognitive evaluation in brain-injured patients and is used more and more frequently in clinical practice to evaluate brain function and predict outcome in severely neurologically impaired patients. Unfortunately, the material used for this procedure is not entirely disposable., Method: We performed a systematic analysis of MDR bacterial contamination in patients contaminated in our ICU using specific bacteriological methods., Results: We report a proven case of cross-contamination of an extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae strain, and a possible case of cross-contamination of a carbapenem-resistant Acinetobacter baumannii strain., Conclusion: Cross-contamination of MDR bacteria is possible through high-density EEG material. However, appropriate procedures can decrease this risk., (Copyright © 2016 British Epilepsy Association. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2016
- Full Text
- View/download PDF
16. [DOhaD and epigenetic information: societal challenges].
- Author
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Rial-Sebbag E, Guibet Lafaye C, Simeoni U, and Junien C
- Subjects
- Epidemiologic Research Design, Female, Humans, Information Storage and Retrieval standards, Interdisciplinary Communication, Life Style, Pregnancy, Disease etiology, Environmental Exposure adverse effects, Environmental Exposure prevention & control, Epigenesis, Genetic, Prenatal Exposure Delayed Effects, Sociological Factors
- Abstract
The concept of the developmental origins of health and disease (DOHaD) alters our understanding of what constitutes "health" or "disease" intended as chronic, non-communicable diseases, which develop over the life course in high income and emerging countries. It implies a change in paradigm forming a basis for prevention policies across the globe. It also impacts psychological, social, economic, ethical and legal sciences. In line with the unanticipated underpinning epigenetic mechanisms are also the social issues (including public policies) that could be produced by the knowledge related to DOHaD that opens a wide field of inquiry. The information unveiled by epigenetics coupled with information on lifestyle including during the development phase, is of unforeseen nature, raising issues of different nature. Therefore it requires specific attention and research, and a specific support by a pluridisciplinary reflection since the very beginning of its production, to anticipate the questions that might be raised in the future., (© 2016 médecine/sciences – Inserm.)
- Published
- 2016
- Full Text
- View/download PDF
17. An improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging.
- Author
-
Yu D, Gustafson WC, Han C, Lafaye C, Noirclerc-Savoye M, Ge WP, Thayer DA, Huang H, Kornberg TB, Royant A, Jan LY, Jan YN, Weiss WA, and Shu X
- Subjects
- Animals, Biliverdine metabolism, Brain Neoplasms metabolism, Crystallography, X-Ray, Drosophila, HEK293 Cells, Heme Oxygenase (Decyclizing) metabolism, Humans, Infrared Rays, Larva, Mice, Microscopy, Confocal, Phytochrome, Rats, Brain Neoplasms diagnosis, Fluorescent Dyes metabolism, Luminescent Proteins metabolism, Neuroimaging methods, Neurons metabolism
- Abstract
Infrared fluorescent proteins (IFPs) are ideal for in vivo imaging, and monomeric versions of these proteins can be advantageous as protein tags or for sensor development. In contrast to GFP, which requires only molecular oxygen for chromophore maturation, phytochrome-derived IFPs incorporate biliverdin (BV) as the chromophore. However, BV varies in concentration in different cells and organisms. Here we engineered cells to express the haeme oxygenase responsible for BV biosynthesis and a brighter monomeric IFP mutant (IFP2.0). Together, these tools improve the imaging capabilities of IFP2.0 compared with monomeric IFP1.4 and dimeric iRFP. By targeting IFP2.0 to the plasma membrane, we demonstrate robust labelling of neuronal processes in Drosophila larvae. We also show that this strategy improves the sensitivity when imaging brain tumours in whole mice. Our work shows promise in the application of IFPs for protein labelling and in vivo imaging.
- Published
- 2014
- Full Text
- View/download PDF
18. Elective non-therapeutic intensive care and the four principles of medical ethics.
- Author
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Baumann A, Audibert G, Guibet Lafaye C, Puybasset L, Mertes PM, and Claudot F
- Subjects
- Ethical Theory, Ethics, Medical, Humans, Life Support Care ethics, Medical Futility, Principle-Based Ethics, Withholding Treatment ethics, Beneficence, Brain Death, Critical Care ethics, Heart Arrest, Personal Autonomy, Respiration, Artificial ethics, Social Justice, Tissue Donors supply & distribution, Tissue and Organ Procurement ethics
- Abstract
The chronic worldwide lack of organs for transplantation and the continuing improvement of strategies for in situ organ preservation have led to renewed interest in elective non-therapeutic ventilation of potential organ donors. Two types of situation may be eligible for elective intensive care: patients definitely evolving towards brain death and patients suitable as controlled non-heart beating organ donors after life-supporting therapies have been assessed as futile and withdrawn. Assessment of the ethical acceptability and the risks of these strategies is essential. We here offer such an ethical assessment using the four principles of medical ethics of Beauchamp and Childress applying them in their broadest sense so as to include patients and their families, their caregivers, other potential recipients of intensive care, and indeed society as a whole. The main ethical problems emerging are the definition of beneficence for the potential organ donor, the dilemma between the duty to respect a dying patient's autonomy and the duty not to harm him/her, and the possible psychological and social harm for families, caregivers other potential recipients of therapeutic intensive care, and society more generally. Caution is expressed about the ethical acceptability of elective non-therapeutic ventilation, along with some proposals for precautionary measures to be taken if it is to be implemented.
- Published
- 2013
- Full Text
- View/download PDF
19. Radiology case of the month. New onset seizures. Pulmonary and estrapulmonary tuberculosis.
- Author
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Gloss DS, Lafaye C, and Neitzschman HR
- Subjects
- Contrast Media, Diagnosis, Differential, Humans, Male, Middle Aged, Magnetic Resonance Imaging, Tomography, X-Ray Computed, Tuberculosis, Central Nervous System diagnosis, Tuberculosis, Pulmonary diagnostic imaging
- Published
- 2010
20. Preliminary crystallographic data of the three homologues of the thiol-disulfide oxidoreductase DsbA in Neisseria meningitidis.
- Author
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Lafaye C, Iwema T, Ferrer JL, Kroll JS, Griat M, and Serre L
- Subjects
- Amino Acid Sequence, Chromatography, Gel, Crystallography, X-Ray, Electrophoresis, Polyacrylamide Gel, Molecular Sequence Data, Protein Conformation, Protein Disulfide-Isomerases isolation & purification, Recombinant Proteins chemistry, Recombinant Proteins isolation & purification, Neisseria meningitidis enzymology, Protein Disulfide-Isomerases chemistry
- Abstract
Bacterial virulence depends on the correct folding of surface-exposed proteins, a process that is catalyzed by the thiol-disulfide oxidoreductase DsbA, which facilitates the synthesis of disulfide bonds in Gram-negative bacteria. Uniquely among bacteria, the Neisseria meningitidis genome possesses three genes encoding active DsbAs: DsbA1, DsbA2 and DsbA3. DsbA1 and DsbA2 have been characterized as lipoproteins involved in natural competence and in host-interactive biology, while the function of DsbA3 remains unknown. In an attempt to shed light on the reason for this multiplicity of dsbA genes, the three enzymes from N. meningitidis have been purified and crystallized in the presence of high concentrations of ammonium sulfate. The best crystals were obtained using DsbA1 and DsbA3; they belong to the orthorhombic and tetragonal systems and diffract to 1.5 and 2.7 A resolution, respectively.
- Published
- 2008
- Full Text
- View/download PDF
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