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1. Gel filtration of hepatitis B surface antigen: increased size of the native particle

Author

R. Y. Dodd and J. A. Kobita

Subjects
HBsAg, Hepatitis B Surface Antigens, Size-exclusion chromatography, virus diseases, Blood Proteins, Biology, Hepatitis B, Hydrogen-Ion Concentration, medicine.disease, Virology, Blood proteins, digestive system diseases, Pepsin A, Sepharose, Molecular Weight, Pepsin, Antigen, biology.protein, medicine, Chromatography, Gel, Humans, Stokes radius
Abstract

The Stokes radius of umpurified hepatitis B antigen (HBSAg) was determined by chromatography on a carefully calibrated Sepharose 4B column. A value of 14-2 nm was found by this procedure, contrasting with a published value of II nm for purified, pepsin-treated HBSAg. Chromatography at pH 3 appeared to reduce the Strokes radius of HBSAg to II nm. Evidence is presented to show that serum proteins adsorbed to HBSAg can be removed with pepsin or acid.

Published
1976

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