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Gel filtration of hepatitis B surface antigen: increased size of the native particle
- Source :
- The Journal of general virology. 33(2)
- Publication Year :
- 1976
-
Abstract
- The Stokes radius of umpurified hepatitis B antigen (HBSAg) was determined by chromatography on a carefully calibrated Sepharose 4B column. A value of 14-2 nm was found by this procedure, contrasting with a published value of II nm for purified, pepsin-treated HBSAg. Chromatography at pH 3 appeared to reduce the Strokes radius of HBSAg to II nm. Evidence is presented to show that serum proteins adsorbed to HBSAg can be removed with pepsin or acid.
- Subjects :
- HBsAg
Hepatitis B Surface Antigens
Size-exclusion chromatography
virus diseases
Blood Proteins
Biology
Hepatitis B
Hydrogen-Ion Concentration
medicine.disease
Virology
Blood proteins
digestive system diseases
Pepsin A
Sepharose
Molecular Weight
Pepsin
Antigen
biology.protein
medicine
Chromatography, Gel
Humans
Stokes radius
Subjects
Details
- ISSN :
- 00221317
- Volume :
- 33
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- The Journal of general virology
- Accession number :
- edsair.doi.dedup.....01f26eb164909e7b63dd891d0ba77cd3