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André AM Torricelli, Jackson B Junior, Marcony R Santhiago, Samir J BecharaDivision of Ophthalmology, University of São Paulo Medical School, São Paulo, BrazilAbstract: Presbyopia, the gradual loss of accommodation that becomes clinically significant during the fifth decade of life, is a physiologic inevitability. Different technologies are being pursued to achieve surgical correction of this disability; however, a number of limitations have prevented widespread acceptance of surgical presbyopia correction, such as optical and visual distortion, induced corneal ectasia, haze, anisometropy with monovision, regression of effect, decline in uncorrected distance vision, and the inherent risks with invasive techniques, limiting the development of an ideal solution. The correction of the presbyopia and the restoration of accommodation are considered the final frontier of refractive surgery. The purpose of this paper is to provide an update about current procedures available for presbyopia correction, their advantages, and disadvantages.Keywords: presbyopia, surgical correction, treatment

Dietary alternatives using insect-based products as an alternative for farm animal nutrition have been the object of study due to the high nutritional value of these feeds and the costs related to both their production and consequently their commercialization. Thus, the use of flies, especially larvae, has a high content of proteins and lipids (fat), as well as minerals and essential nutrients for development and growth, directly impacting the production of these animals, whether meat or milk. Therefore, the objective of this study was to compile data from the literature on the nutritional value of adults and larvae of Black soldier ( Hermetia illucens ) and housefly ( Musca domestica ) as a dietary alternative for animal feed. The Prisma checklist was used. After reviewing the data found in the literature, following the systematic review, it was noted that studies emerge that larvae of black soldier flies and domestic flies of the order Diptera obtain essential sources in the nutrition of ruminants, in addition to obtaining rapid digestibility, thus adhering to reproduction with high nutritional content, due to incident levels of protein, lipids, and minerals in M. domestica and Lucilia sericata , making it a target for inclusion in the diet of farm animals. In addition, it is concluded that both species are studied for their sustainable potential as well as for offering greater economic and nutritional viability when compared to ingredients present in production animal feed.

The aim of this work was to optimize the process of elaborating liver pâtés and omental lamb fat and to evaluate the quality of the products. Livers and fats were obtained from lambs fed with diets composed of corn and soybean meal that were partially replaced by cupuaçu, tucumã and palm kernel cake. To prepare the pâtés, livers were baked for 20 minutes at 100°C, weighed, seasoned, crushed, packaged and pasteurized. The best formulation of the pâté was with 40% liver, 10% fat, 35% water, and pasteurized for 20 minutes at 65°C. The pâté from the livers of lambs fed with palm kernel cake obtained a higher caloric value of 193.05 kcal/100 g and all pâtés met the recommended microbiological quality. There was a significant effect (p< 0.05) of the diets on the aroma and texture of the liver pâtés of lambs fed corn and soybean meal and palm kernel cake, and these were 6.38 and 3.37, respectively. Thus, the pâtés can be considered an alternative to increase the options for consumption of liver from lambs, and also for adding commercial value to lamb viscera., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 Almeida da Silva et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

The evaluation of the reactivity and distress of cattle during corral management, by means of subjective scores, aims at the standardization of behavioral indicators, through non-invasive methods, in addition to enabling the development of more appropriate management practices, thus promoting the comfort and well-being of these animals. Therefore, in this study, we aimed to characterize the temperament and distress of cattle managed in a corral using behavioral indicators during the rainiest period. For this, the experiment was conducted on a property located in the municipality of Mojuí dos Campos, during the rainiest quarter (February-April). Thus, 30 male cattle, not castrated, approximately 29 months of age, clinically healthy, and weighing 310 + 20 kg, were divided into three rearing systems: silvopastoral (SP), traditional (SS), and integrated (SI) systems. There were 10 animals per system. Physiological parameters were collected to evaluate rectal temperature (RT) and respiratory rate (RR), as well as body surface temperature (BST), through thermal windows (head and flank infrared temperature and rump infrared temperature). To evaluate temperament and reactivity, scores indicative of corral behavior were used, namely escape speed (ES), tension score (SS&#95;1), tension score (SS&#95;2), reactivity scale (RS), movement score (MS), and temperament scale (TS). The results showed that there was a thermal amplitude of 5.9 °C on average and 8.6 °C at maximum when comparing the structure of the corral and the trees. In addition, the comparisons between the production systems for the behavioral variables did not differ at the 5% significance level, except for ES, where the traditional system differed from the integrated system and the silvopastoral system, showing intermediate average values for both. In addition, there was a positive correlation between the variables RT and RR (r = 0.72; p < 0.01), RR and SS&#95;2 (r = 0.38; p = 0.04), flank infrared temperature and MS (r = 0.47; p = 0.01), rump infrared temperature and RS (r = 0.37; p = 0.04), SS&#95;1 and RS (r = 0.41; p = 0.02), SS&#95;1 and SS&#95;2 (r = 0.39; p = 0.03), RS and SS&#95;2 (r = 0.58; p = 0.00), RS and MS (r = 0.50; p = 0.01), RS and TS (r = 0.61; p = 0.00), SS&#95;2 and MS (r = 0.51; p = 0.00), SS&#95;2 and TS (r = 0.47; p = 0.01), and MS and TS (r = 0.44; p = 0.02), and a negative correlation between ES and TS (r = -0.42; p = 0.02). The rainy season had a major influence on the evaluation of temperature and distress levels during handling in the corral, as evidenced by the association between physiological and behavioral parameters.

Thus, this review aims to deepen the understanding of buffalo farming in the Amazon, presenting the quality and nutritional value of buffalo meat and liver. This information serves as a subsidy to improve practices related to the breeding system, nutrition, health and sustainability associated with aquatic buffaloes. For this, a review of the databases was carried out using the descriptors "nutritional value of buffalo meat", "nutritional value of buffalo liver" and "buffalo breeding in the Amazon". Thus, the consumption of foods derived from aquatic buffaloes has important nutritional value for human consumption. In view of this, it is possible to conclude that the nutrition of these animals is influenced by the biodiversity of the Amazon, giving unique characteristics to its products, also highlighting the importance of carrying out research that aims to value the potential use of this species and strengthen the economy of the region.

The objective of this study was to evaluate the thermal comfort of Nelore cattle ( Bos indicus ) managed in silvopastoral and traditional systems associated with rumination behavior in a humid tropical environment in the Eastern Amazon, Brazil. The study was carried out on a rural property in Mojuí dos Campos, Pará, Brazil, during the transition period of the year, from June to July 2023. Over these two months, six consecutive data collection days were held. We selected 20 clinically healthy non-castrated male Nelore cattle, aged between 18 and 20 months, with an average weight of 250 kg and body condition score of 3.5 (1-5). These animals were randomly divided into two groups: traditional system (TS) and silvopastoral system (SS). The physiological variables evaluated included RR, RT and BST. The variables were analyzed using the linear mixed model. For agrometeorological variables, higher values were observed between 10:00 a.m. (33 °C) and 6:00 p.m. (30 °C), with the highest temperature observed at 4:00 p.m. (40 °C). The RR showed interactions ( p = 0.0214) between systems and times; in general, higher RR were obtained in the Traditional. The animals' RT showed no significant difference ( p < 0.05) between the production systems, but there was a statistically significant difference in relation to the time of collection ( p < 0.0001). In the BGHI, it was possible to observe that there was mild stress in the period from 22:00 at night to 6:00 in the morning and moderate stress in the period of greatest increase in temperature, from 10:00 in the morning to 18:00 at night. BST showed no statistical difference between the regions studied or between the SP (35.6 °C) and TS (36.25 °C) systems. RT in the TS showed a positive correlation with AT (r = 0.31507; p = 0.0477). RT in the SP showed a positive correlation with THI (r = 35583; p = 0.0242). On the other hand, RT in the SP (r = 0.42873; p = 0.0058) and ST (r = 0.51015; p = 0.0008) showed a positive correlation with BGHI. RR in the TS showed a positive correlation with BGHI (r = 0.44908; p = 0.0037). The greatest amounts of rumination were carried out by animals in the SP system, generally ruminating lying down ( p < 0.05). With regard to rumination behavior in the morning and afternoon, there were higher numbers of WS and LD in the TS ( p > 0.05). Most of the time, the cattle were LD during the morning and afternoon shifts, and at night and dawn they were WS in the TS. Therefore, the SP offers more thermal comfort advantages compared to the TS system.

The objective of this study was to evaluate the effect of the transport distance and lairage time on behavioral indicators of zebu beef cattle in the resting pen of a slaughterhouse using the focal animal technique. Eight lots of male zebu cattle, Nelore, aged approximately 4 years old, weighing on average 500 ± 28.5 kg-1, from different municipalities, transported by land, were evaluated. The lots were grouped into two categories: short distance (> 500 km) and long distance (> 500 km). Five focal animals per evaluated lot were used in each cycle of behavior observation, randomly chosen, and visually identified. The animal focal sampling method was used, with instantaneous recording, every 5 min per animal/h. A video camera was used and placed at a fixed point in the corral. After slaughtering, bruises and carcass pH were evaluated. The respiratory rate (RR) was measured in two moments, 30 min after unloading (RR&#95;I) and 1 h before slaughtering (RR&#95;F); behavioral data were divided into four lairage periods (1st, 2nd, 3rd, and 4th) equally and evaluated as repeated measures over time. The lairage time lowered the behaviors of headbutting (NCHBs), pushing (PsH), mounting (MT), and chasing away (CA), ( p  = 0.0001), and these behaviors were more evident in animals transported for short distances in the initial resting times, decreasing as they remained in the resting pen. There was effect of distance on the initial and final pH of the carcass, being greater in the short distance ( p  = 0.047). There was no effect of distance or lairage time on initial or final RR ( p  > 0.05). In addition, animals transported over long distances showed less carcass injuries, which were observed during post mortem inspection. It is concluded that animals transported over short distances present a greater number of agonistic behaviors, such as NCHBs, MT, PsH, and CA, predisposing to higher rates of bruising during lairage and more accentuated changes in the final pH of the meat., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Silva, Gouveia Júnior, Damasceno Neto, Sousa, Barbosa, Bruno Rebelo da Silva, Santos, Lourenço-Júnior, Camargo Júnior and Silva.)

The nutritional composition of bovine liver, particularly in terms of vitamins E, β-carotene, and cholesterol concentration, is significantly influenced by the cattle-rearing system and diet. This study aimed to elucidate the impact of four predominant cattle-rearing systems in the Eastern Amazon region on the vitamin E, β-carotene content, and cholesterol levels in bovine liver during the rainy season. Liver-tissue samples were collected from 48 cattle, with twelve specimens representing each rearing system. The systems encompassed two native pastures located in flood-prone areas, cultivated pastures on upland terrain and a confinement-rearing system. Our findings indicate that, when considering all rearing systems collectively, there were no significant differences in the levels of vitamins and cholesterol in the liver tissues ( p > 0.05). However, a marked disparity in cholesterol levels emerged when comparing intensive rearing systems to extensive ones, with the former demonstrating notably higher concentrations ( p = 0.01). Additionally, the intensive rearing system was associated with elevated levels of β-carotene ( p < 0.01), α-tocopherol ( p = 0.01), and β-tocopherol ( p = 0.01) relative to the extensive systems. No significant variations were observed amongst the different extensive systems ( p > 0.05). These results suggest that, while all rearing systems yielded liver tissues with typical concentrations of vitamins and cholesterol, the intensive rearing system led to a higher accumulation of certain vitamin compounds. This study highlights the substantial nutritional implications of different cattle-rearing systems in the Eastern Amazon and provides valuable insights for developing dietary strategies to optimize the nutritional quality of bovine liver. Therefore, the generated results are groundbreaking in the Eastern Amazon, Brazil, and inspire the development of new research projects to address other demands in this field and achieve additional outcomes.

Variations in environments, including climate, diet, and agricultural practices, significantly impact the composition and microbial activity. A profound understanding of these adaptations allows for the improvement of nutrition and ruminant production. Therefore, this review aims to compile data from the literature on the rumen microbiota and molecular techniques for identifying the different types of microorganisms from the rumen fluid of ruminants. Analyzing the literature on rumen microbiology in different ruminants is complex due to microbial interactions, influenced by the environment and nutrition of these animals. In addition, it is worth noting that the genera of protozoa and fungi most evident in the studies used in this review on the microbiology of rumen fluid were Entodinium spp. and Aspergillus spp., respectively, and Fibrobacter spp. for bacteria. About the techniques used, it can be seen that DNA extraction, amplification, and sequencing were the most cited in the studies evaluated. Therefore, this review describes what is present in the literature and provides an overview of the main microbial agents in the rumen and the molecular techniques used.

The objective was to characterize the pastures by grazing cycle, as well as to evaluate the performance of buffaloes in intensive rotational grazing in a silvopastoral system in the eastern Amazon supplemented with agro-industry co-products in order to characterize the grazing cycles, the composition of the fractions, and the carcass yield. Fifteen non-castrated, crossbred water buffaloes (Murrah × Mediterranean) were used. All animals used in the study were clinically healthy and weighed approximately 458 kg. The animals were grazed in a single group, and supplementation (1% of live weight-LW/day) was divided into three treatments: control (control-conventional ingredients); Cocos nucifera coconut cake ( Cocos nucifera ) (coconut cake-70%); and palm kernel cake ( Guinean elaeis ) (palm kernel cake-70% palm kernel cake). The chemical composition of the forage is different in each part of the plant, with higher protein values in the leaves (11.40%) and higher acid detergent fiber (ADF) values in the stems (50.03%). Among the ingredients of the supplement, corn has the highest percentage of indigestible protein (35.57%), most of the protein in palm kernel cake is B3 (49.11%), and in Coco, B2 (51.52%). Mombasa grass has a higher percentage of B3 and B2 proteins; the indigestible fraction is lower in the leaves (17.16%). The leaf/stem ratio also varied between grazing cycles, being better in the second cycle (2.11%) and with an overall average of 1.71. Supplement consumption varied between cycles and was higher in the control treatment, with an overall mean of 4.74. There was no difference in daily weight gain and carcass yield, with an average of 1 kg/day and 49.23%, respectively. Therefore, it can be concluded that including supplements based on by-products from the coconut and palm oil agro-industries promotes performance and carcass yields compatible with conventional supplements. Their use reduces production costs, optimizes the utilization of forage mass, enhances the sustainability of the production chain, and, therefore, is recommended.

The El Niño represents a substantial threat to pastures, affecting the availability of water, forage and compromising the sustainability of grazing areas, especially in the northern region of Brazil. Therefore, the objective of this study was to characterize the thermographic profile of three production systems in the Eastern Amazon, Brazil. The study was conducted on a rural cattle farm in Mojuí dos Campos, Pará, Brazil, between August and November 2023. The experiment involved livestock production systems, including traditional, silvopastoral and integrated, with different conditions of shade and access to the bathing area. An infrared thermographic (IRT) camera was used, recording temperatures in different zones, such as areas with trees, pastures with forage and exposed pastures. The highest mean temperatures ( p = 0.02) were observed in pastures with full sun from August to November. On the other hand, the lowest average temperatures were recorded in areas shaded by chestnut trees ( Bertholletia excelsa ). The highest temperature ranges were found in sunny areas and the lowest were recorded in shaded areas. The highest temperatures were observed in the pasture in full sun, while the lowest were recorded in areas shaded by chestnut trees ( p < 0.0001). The interaction between the systems and treatments revealed significant temperature differences ( p < 0.0001), with the native trees showing an average temperature of 35.9 °C, lower than the grasses and soil, which reached 61.2 °C. This research concludes that, under El Niño in the Eastern Amazon, areas shaded by Brazil nut trees had lower temperatures, demonstrating the effectiveness of shade. Native trees, compared to grasses and soil, showed the ability to create cooler environments, highlighting the positive influence on different species such as sheep, goats and cattle.

In ruminants, diet composition has a positive correlation with heat production, which can influence thermoregulation, energy expenditure and, consequently, animal performance. The objective of this work was to evaluate the effects of climatic conditions and supplementation based on palm kernel cake, on the thermoregulation of crossbred buffaloes in the eastern Amazon. The research was carried out at Embrapa Amazônia Oriental (01°26' S and 48°24' W), Belém, Pará, and lasted 12 months (representing the entire year). Twenty-four buffaloes, females, with initial age and an average weight of 54 ± 7 months and 503.1 ± 23 kg, respectively, non-pregnant, non-lactating and clinically healthy were used, divided into four treatments based on the supplementation content of the palm cake (%DM) in relation to their body weight (%): 0, 0.25, 0.50 and 1.0. The animals were kept in paddocks with Brachiaria brizantha (cv. Marandu), in a rotating system, with water to drink and mineral salt ad libitum. Equipment was installed to record environmental data (temperature and relative humidity, dew point temperature, wet bulb and black globe) and physiological data: rectal temperature (RT); respiratory rate (RR); and body surface temperature (BST), recorded twice a day, always in the morning (6:00 a.m. to 7:00 a.m.) and afternoon (12:00 p.m. to 1:00 p.m.) shifts, and were used to calculate the Globe Temperature and Humidity Index (GTHI). Supplementation did not influence the physiological variables of thermoregulation ( p > 0.05). However, there were differences in the GTHI between the shifts, with higher means in the afternoon shift, especially in the less rainy period of the year, where the GTHI reached 92.06 ± 2.74 ( p < 0.05). In all periods of the year, the mean values of RT, RR and BST were higher in the afternoon shift ( p > 0.05). The respiratory rate (RR) is associated with the annual seasonality of the thermal waters, with higher averages in the afternoons of the rainy season. The positive correlation for rectal temperature, respiratory rate and body surface temperature indicated that buffaloes respond to thermal elevations in the atmosphere (afternoon period) and, consequently, reflect on the GTHI. Supplementation does not influence thermoregulation; the changes observed occurred in response to the region's thermal and rainfall conditions (mainly in the afternoon shift), with higher GTHI values.

The Amazon has a wide variety of oilseeds that generate a huge amount of co-products with potential for use in animal nutrition. The objective was to use alternative resources (oilseed cakes) in the feeding of lambs to assign a sustainable destination to this biomass, and evaluate its influence on the quality and fatty acid (FA) profile of the meat. Twenty-four lambs, male, castrated, crossbred Dorper × Santa Inês, weighing 30 ± 1.3 kg of initial body weight, were distributed in a completely randomized design in 4 treatments (diets) with six replications (animals). The control diet (Control) contained corn and soybean meal as main ingredients, which were partially replaced in the other diets by cupuassu cake diet (Cup), palm kernel cake diet (Palm) and tucuma cake diet (Tuc). The inclusion of Amazon cakes influences the lipid (P = 0.02) and protein (P < 0.01) composition of meat (longissimus lumborum); reduces cooking losses (P < 0.01); influences the colors (L, a, b), chroma, and Hue Angle (P < 0.01); promotes changes in total FA composition and FA profile (P < 0.05); reduces hypocholesterolemic FA (h) (P = 0.01), but does not influence hypercholesterolemic (H) and indices h:H, AI and TI (P > 0.05). The inclusion of oilseed cakes influences the chemical composition, physical parameters, composition and fatty acid profile of the meat, but does not influence the indicators of atherogenicity, thrombogenicity and cholesterolemia., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2023 de Castro et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

Increasing the efficiency of rumen fermentation is one of the main ways to maximize the production of ruminants. It is therefore important to understand the ruminal microbiome, as well as environmental influences on that community. However, there are no studies that describe the ruminal microbiota in buffaloes in the Amazon. The objective of this study was to characterize the rumen microbiome of the water buffalo ( Bubalus bubalis ) in the eastern Amazon in the dry and rainy seasons in three grazing ecosystems: Baixo Amazonas (BA), Continente do Pará (CP), Ilha do Marajó (IM), and in a confinement system: Tomé-Açu (TA). Seventy-one crossbred male buffaloes (Murrah × Mediterranean) were used, aged between 24 and 36 months, with an average weight of 432 kg in the rainy season and 409 kg in the dry season, and fed on native or cultivated pastures. In the confinement system, the feed consisted of sorghum silage, soybean meal, wet sorghum premix, and commercial feed. Samples of the diet from each ecosystem were collected for bromatological analysis. The collections of ruminal content were carried out in slaughterhouses, with the rumen completely emptied and homogenized, the solid and liquid fractions separated, and the ruminal pH measured. DNA was extracted from the rumen samples, then sequenced using Restriction Enzyme Reduced Representation Sequencing. The taxonomic composition was largely similar between ecosystems. All 61 genera in the reference database were recognized, including members of the domains Bacteria and Archaea. The abundance of 23 bacterial genera differed significantly ( p < 0.01) between the Tomé-Açu confinement and other ecosystems. Bacillus , Ruminococcus , and Bacteroides had lower abundance in samples from the Tomé-Açu system. Among the Archaea, the genus Methanomicrobium was less abundant in Tomé-Açu, while Methanosarcina was more abundant. There was a difference caused by all evaluated factors, but the diet (available or offered) was what most influenced the ruminal microbiota.

The diet offered to animals has a great influence on the composition of tissues and, consequently, the quality. The objective of this study was to evaluate the influence of Amazonian ecosystems, in the dry and rainy periods of the year, on the composition of cholesterol, tocopherols, β-carotene and the fatty acid profile of the livers of water buffaloes ( Bubalus bubalis ) reared in the Eastern Amazon, in an extensive or intensive system. Total lipid content was influenced by the location and time of year ( p < 0.05). Ninety-six male water buffaloes were used (12 per sampling period), aged between 24 and 36 months, with average weights of 432 kg (end of the rainy season) and 409 kg (end of the dry season). Total cholesterol, α-tocopherol and γ-tocopherol influenced the relationship between extensive vs intensive ecosystems, location, periods and the interaction between the location and period of the year ( p < 0.05). Animals raised in a pasture ecosystem had the highest values of omega-3, and those raised in confinement, the highest values of omega-6 ( p < 0.05). The proportions of n-6/n-3 and hypocholesterolemia (7.14) and hypercholesterolemia (3.08%) (h/H) were found in greater amounts in animals raised in confinement ( p < 0.05). The atherogenic index (AI) had a higher value in the rainy season, in animals raised in Santarém (2.37%), with no difference between pasture and feedlot ecosystems, except in animals raised in the rainy season in Nova Timboteua, with a lower AI (1.53%). The thrombogenicity index (TI) was higher in the livers of confined animals (0.32%) and lower (0.18%) in those raised in Nova Timboteua (rainy season). Amazonian ecosystems influence the nutritional values of buffalo liver, with the best nutritional values in animals in the extensive system.

Background: Next-generation sequencing has had a significant impact on genetic disease diagnosis, but the interpretation of the vast amount of genomic data it generates can be challenging. To address this, the American College of Medical Genetics and Genomics and the Association for Molecular Pathology have established guidelines for standardized variant interpretation. In this manuscript, we present the updated Hospital Israelita Albert Einstein Standards for Constitutional Sequence Variants Classification, incorporating modifications from leading genetics societies and the ClinGen initiative., Results: First, we standardized the scientific publications, documents, and other reliable sources for this document to ensure an evidence-based approach. Next, we defined the databases that would provide variant information for the classification process, established the terminology for molecular findings, set standards for disease-gene associations, and determined the nomenclature for classification criteria. Subsequently, we defined the general rules for variant classification and the Bayesian statistical reasoning principles to enhance this process. We also defined bioinformatics standards for automated classification. Our workgroup adhered to gene-specific rules and workflows curated by the ClinGen Variant Curation Expert Panels whenever available. Additionally, a distinct set of specifications for criteria modulation was created for cancer genes, recognizing their unique characteristics., Conclusions: The development of an internal consensus and standards for constitutional sequence variant classification, specifically adapted to the Brazilian population, further contributes to the continuous refinement of variant classification practices. The aim of these efforts from the workgroup is to enhance the reliability and uniformity of variant classification., (© 2023. The Author(s).)

Genetic group, age at entry into confinement and at slaughter, are characteristics that have an important influence on lamb performance and carcass. The aim of this study was to evaluate the performance, carcass characteristics and non-carcass components from different genetic groups (Santa Inês and ½ Dorper x ½ Santa Inês) sheep, submitted to different feedlot entry and exit strategies. Were used 72 lambs males and castrated; 36 Santa Inês (SI) and 36 crossbred (Dorper x Santa Inês-DSI), with 6 months of average initial age. The groups were established in a completely randomized experimental design, in a 2x3x4 factorial arrangement, from the combination of genetic groups (GG), body weight at the beginning of confinement (WBC) and length of stay in confinement (LSC). The body weight classes at the beginning of confinement were: light (25 kg), intermediate (28 kg) and heavy (31 kg), for Santa Inês and crossbreeds, respectively. Slaughters were carried out every 28 days of confinement, in four LSC: 0, 28, 56 and 84 days. The GG did not influence performance, carcass and non-carcass component traits of lambs (p > 0.05). There was an effect of the WBC on the weights: final (FW), metabolic (MW), body at slaughter (BWS), empty body (EBW), hot carcass (HCY) and cold (CCW), loin, shoulder, leg musculature; loin eye area (LEA) and loin fat (p < 0.05). There was also an effect on LSC, for FW, average daily weight gain (ADG), MW, weight and yield of body components, weight of cuts and tissue ratio components of cuts (p < 0.05). In non-carcass components, effect on full and empty weight of: omasum, rumen-reticulum, small intestine; empty large intestine, liver and kidneys, paws and skin, and perirenal, pelvic and inguinal fat (p < 0.05). Interaction double effect on the tissue muscle/fat:bone ratio (MF:B) and for the full omasal component (p < 0.05). And triple interaction effect for ADG, full omasum and perirenal fat (p < 0.05). Weight at the beginning of confinement and confinement time are the characteristics that most influence performance, quantitative characteristics of carcass and non-carcass components. Regardless of the genetic group and age class, the animals reach the same weight after 84 days of confinement. Thus, the confinement of heavier lambs (31 kg) can be a profitable alternative, as they presented the highest weights for the most commercially valued cuts (shank and loin). The confinement strategy must adapt to market situations., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2023 Pereira et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

This study aims to present a proposal for using the focal animal recording technique to evaluate the welfare of buffaloes and to verify the association between each behavior and thermal comfort indices. The study was conducted in an experimental paddock located in Santarém, Pará, Brazil. A total of 10 female Murrah animals were used. The behavior of the animals was recorded during the day, with the use of three trained observers, for 72 consecutive h. Climatic variables were collected, and the Temperature-Humidity Index (THI) and the practical Buffalo Comfort Climatic Conditions Index (BCCCIp) were determined. The multivariate technique of principal components and Spearman's correlation were employed. BCCCIp and THI were outside the thermal comfort zone at different times of the day. Grazing (P) was more frequent in the coldest hours of the day, while rumination occurred at different periods, mainly during the daytime and frequently in a lying position. There was a positive correlation between idle lying behavior and average temperature-Tmed ( r  = 0.583; p  < 0.028), THI ( r  = 0.432; p  < 0.034), and BCCCIp ( r  = 0.554; p  < 0.049). There was a positive correlation between grazing and Tmed ( r  = 0.665; p  < 0.0004) and BCCCIp ( r  = 0.583; p  < 0.036). The standing idle behavior negatively correlated with Tmed ( r  = -0.718; p  < 0.0001), THI ( r  = -0.522; p  < 0.008), and BCCCIp ( r  = -0.8076; p  < 0.0008). The lying ruminating behavior had a positive correlation with Tmed ( r  = 0.586; p  < 0.002), THI ( r  = 0.477; p  < 0.018), and BCCCIp ( r  = 0.8033; p  < 0.0009). Furthermore, ruminating while standing correlated positively with Tmed ( r  = 0.680; p  < 0.0003). The adaptation of the focal animal technique, with six observers evaluating each animal for 6 min through filming, proved to be efficient in pointing out the different behaviors of buffalo raised in Eastern Amazon fields under heat stress at different times of the day., Competing Interests: WS, JL-J, RC, and AS were employed by Brazilian Agricultural Research Corporation. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Silva, Silva, Gouveia Júnior, de Alvarenga, Barbosa, Silva, Pereira dos Santos, Lourenço-Júnior, Camargo Júnior and Silva.)

Background: Trichoscopy can be very useful for evaluation for hair transplantation (HT), helping rule out simulators of androgenetic alopecia (AGA). There are only a few reports about normal trichoscopic findings after HT., Objective: The aim of this study was to evaluate donor and recipient area trichoscopy after FUE in HT without complications in AGA patients., Method: A prospective study was carried out with 10 patients, for 24 weeks, with photographic follow-up, using FotoFinder ® . Patients were evaluated from week 1 (w1) to week 8 weekly, then, at week 12 and week 24 post-HT., Results: Main results were perifollicular crusts, donor area erythema, recipient area erythema, perifollicular erythema, white circles, perifollicular whitish halos, repilation black dots, dystrophic hairs, folliculitis, yellow dots. We discussed the meaning of each of these findings in the period in which they occurred., Conclusion: Normal trichoscopic findings in post-transplant patients have not been well established yet. Our findings are an attempt to define a normal pattern. Future studies with a longer follow-up may be necessary to corroborate these results., Competing Interests: The authors have no conflicts of interest to declare., (© 2023 S. Karger AG, Basel.)

In the Lower Amazon mesoregion, there are basically three types of production systems: the traditional (without shade and no bathing area), the silvopastoral (with shade and no bathing area), and the integrated (with shade and bathing area). It is considered that the type of production system influences the thermal comfort and productivity of cattle, so this research aims to evaluate the influence of these three types of production systems on the thermoregulation of Nellore cattle. The experiment was carried out on a rural property for raising cattle, located in Mojuí dos Campos, Pará, Brazil, during the transition period (June/July). Thirty bovine males (not castrated, aged between 18 and 20 months, average weight of 250 ± 36 kg, body condition score of 3.5, clinically healthy) were randomly divided into three groups: Silvopastoral System-SS ( n = 10), Traditional System-TS ( n = 10), and Integrated System-IS ( n = 10). Climate variables were collected (air temperature (AT °C), relative humidity (RH %), wind speed (WS, m/s), solar radiation (SR), black globe temperature (BGT °C), and physiological parameters, such as respiratory rate (RR) and rectal temperature (RT)) at 6 a.m., 12 p.m., 6 p.m., and 12 a.m. to determine the thermal comfort situation of the animals. Thermographic images of the environment and animals were captured in order to obtain the body surface temperature (BST) through infrared thermography. The Benezra Thermal Comfort Index (BTCI), Environmental Stress Index (ESI), Equivalent Temperature Index (ETI), and Iberian Heat Tolerance Index (Iberian HTI) were used. The results showed that the silvopastoral system, with shading by chestnut trees and an ample vegetative area, presented better thermal conditions, with an average of 28.98 °C, in comparison with the traditional system (35.93 °C) and the integrated one (34.11 °C). It was observed that the body surface temperature of cattle did not differ significantly between the anatomical regions of the body and the studied systems ( p > 0.05). As for the respiratory rate, the traditional system registered higher values, with an average of 41 movements per minute, indicating possible thermal stress ( p < 0.05). The thermal comfort indices revealed that all systems presented moderate stress conditions during times of higher solar intensity. It is concluded that the silvopastoral system proved to be more favorable for cattle, providing shade and reducing thermal stress, which may have a positive impact on animal welfare and productivity in this region.

The good productive and reproductive performance of the animals depends on multiple factors, including favorable climatic conditions, which are responsible for causing changes in the physiological and behavioral responses. Thus, the objective of this study was to evaluate the temperature and humidity index (THI) to support the implementation of a rearing system in ruminants in the Western Amazon, Brazil. Monthly temperature and relative humidity data were obtained from the Database for Teaching and Research (BDMEP) for the capitals Manaus (Amazonas), Boa Vista (Roraima), and Rio Branco (Acre), considering a historical series of 27 years (1993 to 2020), referring from January to December. In the capital Boa Vista, the months of January, February, May, June, and July showed an indication of mild stress and the months of March, April, August, September, October, November, and December had moderate stress. In Rio Branco, all months of the year presented the average THI in mild stress with variations of lower THI (73) and higher THI (77). In the capital Manaus, the months from January to July signaled mild stress, but from August to November, there was moderate stress, and in December, there was mild stress. It is possible to observe significant climatic variations during the months as well as the years of study, with the animals under thermal stress with THI > 72 or in a warning signal, with a gradual increase in temperature and humidity indices over the last 10 years. The importance of the breeding system and the consideration of environmental factors, such as the THI, are fundamental for the wellbeing and performance of cattle raised in the field. Our results support the adoption of heat stress mitigation strategies for ruminants in Western Amazon., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Silva, Printes, Lima, Silva, dos Santos, Camargo Júnior, Barbosa, Silva, Silva, Silva, Araújo, Britto and Lourenço-Júnior.)

In Brazil, the study on equine thermoregulation has not shown considerable advances, moreover, in the Amazon, this is a little explored aspect. This study aims to evaluate the thermoregulation of horses submitted to two methods of post-exercise cooling, in the climatic conditions of the Eastern Amazon. The experiment was conducted at Centro Hipico, Ananindeua, Para, for 15 days. Ten male horses, castrated of the Brazilian breed, aged 13 years and with an average weight of 482.3 kg were used. Equestrianism, which was exercised with pre-established protocol in the arena and track, for 30 min. After the exercise, the animals were divided into two groups for application of the treatments, which were two cooling methods, such as a bath with water at room temperature (about 25°C) and a hiper cooling method (6-9°C). During the experimental period, air temperature (AT) and relative humidity (RH) data were recorded and the Temperature and Humidity Index (THI) was calculated. Data from the rectal temperature (RT), heart rate (HR), respiratory rate (RR) and Body surface temperature (BST) of the animals were also measured at three moments: before exercise, after exercise and after applying the cooling methods. The adaptability index used was the Benezra Thermal Comfort Index (BTCI). The BST was performed with the help of infrared thermograph, which were the left side of the neck, thorax, rump, and right side to the armpi, before the exercise, after the exercise and after applying the cooling methods. The statistical design was completely randomized. Analysis of variance was performed using the GLM Procedure of SAS 9.1.3. After the application of the cooling techniques in the animals, the AT and THI were higher and the highest RH values were found before the exercise (87.21%). The highest RT, RR, HR, BST, and BTCI values were observed after exercise. There was no significant dierence ( P > 0.05) between cooling methods. There was a highly significant and positive correlation ( P < 0.01) between all physiological variables (RT, RR, HR, and BST) and the Iberian Index with AT and THI and negative with RH ( P < 0.01), thus demonstrating the influence of the environment on the thermoregulation of animals. It is concluded the evaluation of stress, welfare and thermoregulation of horses submitted to two methods of post-exercise cooling, in the climatic conditions of the Eastern Amazon, demonstrated that the studied cooling methods showed to reduce the rectal temperature, the respiratory rate, the heart rate and the body surface temperature with equal efficiency. However, in terms of practical ease of use, the room temperature water cooling method has proven to be more practical., Competing Interests: The authors declare that the research was carried out in the absence of any commercial or financial relationships that could be interpreted as a potential conflict of interest., (Copyright © 2023 Lisboa, Silva, Silva, Barbosa, Silva and Lourenço-Júnior.)

Thermal stress causes severe effects on the wellbeing and reproduction of cattle, including changes in oogenesis and spermatogenesis, generating great concerns, which last for decades. In cattle, the occurrence of thermal stress is associated with a reduction in the production of spermatozoids and ovarian follicles, in addition to the increase of major and minor defects in gametes or in their intermediate stages. In bovine females able to reproduce, a reduction in the rate of estrus manifestation and an increase in embryonic mortality has been observed. Therefore, keeping animals on good welfare conditions, with water supply and in shaded areas can favor the improvement of different reproductive parameters. For all this, the present study aimed to gather, synthesize and argue recent studies related to animal welfare, focusing on the effects of thermal stress on the reproduction of cattle, aiming to support possible strategies to mitigate the harmful effects of thermal stress in this species., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Silva, Silva, Camargo-Júnior, Silva, Santos, Viana, Silva, Silva and Lourenço-Júnior.)

Bovine tuberculosis (TB) is a chronically evolving zoonotic infectious disease caused by Mycobacterium bovis. Anatomopathological examination during post mortem inspection in bovines is the main resource engaged in sanitary slaughter; however, it is very troublesome since many granulomatous inflammatory processes have similar morphological characteristics. Thus, this study aims to use complementary diagnosis methods (histopathological and polymerase chain reaction - PCR assays) to confirm the macroscopic assessment of lymphadenopathies indicative of tuberculosis in bovines slaughtered in a refrigerated slaughterhouse in Tailândia city, PA, Brazil. Fifty‑one samples were collected from lesions indicative of tuberculosis in pre‑scapular and pre‑pectoral lymph nodes (or different lymphadenitis) in condemned carcasses. Histological processing employed routine techniques carried out at the Laboratory of Animal Pathology of the Federal Rural University of the Amazon, while the PCR assay was performed at the Bacteriology Laboratory of the Evandro Chagas Institute. Results showed that 1.96% of the histopathology samples corresponded to inflammatory processes typical of TB and that, in PCR, 4.25% of the samples had the amplification profile of the M. bovis species. These results indicate the importance of adding complementary methods to assist the sanitary inspection line and make inspection more efficient in its decisions.

This study aimed to evaluate the influence of different production ecosystems, three in native and cultivated pastures (extensive), at two seasons of the year (dry and rainy), and one in confinement (intensive) in the Eastern Amazon, on the mineral content of buffalo liver raised on these ecosystems. Twelve male buffalo ( n = 12), aged between 24 and 36 months, slaughtered in commercial slaughterhouses, were used in each of the ecosystems considered: Marajó; Lower Amazon; Cultivated Pasture, and in confinement system, Pará, Brazil. Approximately 5 g of liver was collected, stored and frozen until lyophilization. Samples were analyzed for mineral content based on inductively coupled plasma optical emission spectrometry (ICPOES) readings. The relationship between extensive ecosystems and an intensive production system ( p < 0.05) in the values of potassium (K), iron (Fe), copper (Cu), manganese (Mn) and barium (Ba) was evaluated. The different ecosystems studied influenced ( p < 0.05) the mineral values found in the liver of buffaloes raised in the ecosystems, for sodium (Na), K, calcium (Ca), magnesium (Mg), sulfur (S), copper (Cu), iron (Fe), manganese (Mn) and barium (Ba). The period of the year interacted with the values of Na, K, S and Cu; however, an interaction of local vs. period of the yer was observed for the values of K, Mg, P, S and Cu. It can be concluded that the buffalo liver is an excellent source of minerals and can be included in the human diet and that the ecosystem the animals are raised influences its content.

Healthy food must have an adequate balance of macroelements, such as calcium or phosphorus and, microelements, such as iron, copper. This study aimed to evaluate the influence of three extensive systems, during the dry and rainy seasons, and an intensive (feedlot) system in the Eastern Amazon, on the muscle mineral profile of water buffaloes. In total, 12 male buffaloes, aged between 24 and 36 months, slaughtered in commercial slaughterhouses, were used in each of the systems considered: Marajó island, Santarém, Nova Timboteua, and a feedlot. Approximately 5 g of muscle was collected, stored, and frozen, until freeze-dried. The samples were analyzed for the mineral profile using inductively coupled plasma-optical emission spectrometry (ICP-OES). There were significant differences ( P < 0.05) for concentrations of sodium (Na), magnesium (Mg), phosphorus (P), sulfur (S), copper (Cu), zinc (Zn), and iron (Fe). Extensive and intensive systems showed significant differences ( P < 0.05) for Na, Ca, S, Cu, and Fe concentrations. The season also influenced ( P < 0.05) K, Ca, P, S, Zn, and Fe concentrations. The location and season of the year had a significant interaction ( P < 0.05) for K, Mg, P, Zn, and Fe concentrations. The study showed that the different Amazonian production systems and the year season influenced the levels of minerals present in buffalo muscle. The values obtained were, in general, higher in extensive production systems, and Marajó Island stood out with higher mineral values in the dry season. Therefore, the meat from animals reared in these systems is a good mineral source for daily human needs., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Rodrigues, Silva, Lourenço-Júnior, Silva, Almeida, Mourato, Castro, Bezerra, Silva and Prates.)

The objective was to evaluate the effects of palm kernel cake (PKC) supplementation on voluntary feed intake, in situ rumen degradability and performance in the wettest (WS-January to June) and less rainy seasons (LR-July to December) in the eastern Amazon. A total of 52 crossbred buffaloes that were neither lactating nor gestating were used, with 24 for the LR, aged 34 ± 04 months and an initial average weight of 503 ± 48 kg, and 24 for the WS aged 40 ± 04 months with an average weight of 605 ± 56 kg. The four treatments (levels of PKC in relation to body weight) were distributed in a completely randomized design, with 0% (PKC0), 0.25% (PKC0.2), 0.5% (PKC0.5) and 1% (PKC1) with six repetitions. The animals were housed in Marandu grass paddocks, intermittently, with access to water and mineral mixture ad libitum. Degradability was evaluated by the in situ bag technique in four other crossbred buffaloes with rumen cannulae, in a 4 × 4 Latin square (four periods and four treatments). The inclusion of PKC increased supplement consumption and production of ether extracts and reduced the intake of forage and non-fibrous carbohydrates. The dry matter degradability of Marandu grass was not affected; however, the fermentation kinetics in neutral detergent fiber (NDF) differed between the treatments. The co-product dry matter colonization time was greater in PKC1 and the highest effective degradability rates were from PKC0, but the productive performance of the animals was not influenced. Supplementation of buffaloes with PKC is recommended for up to 1% of body weight.

Body weight records were used to characterize the growth curve of Nelore cattle. Body weight was regressed as a function of age, for both sexes, by using nonlinear models through the functions of Brody, Gompertz, Logistic, Richards, Meloun 1, Von Bertalanffy, and Von Bertalanffy. The quality of the model arrangements was evaluated by employing Akaike and Bayesian Schwarz information criteria. The Brody function provided the best adaptations by the evaluators and, considering the asymptotic weight and the maturation rate as random, a reduction in residual variance of 79% for males and 83% for females was obtained in relation to the models under fixed contexts. In males, the absolute and relative growth rates ranged from 0.921 to 0.261 kg/day and 2.39 to 0.08%, respectively. For the same rates, under another approach, females ranged from 0.922 to 0.198 kg/day and 2.55 to 0.06%, respectively. Males showed greater growth acceleration at the beginning of the growth trajectory, being equal to females at 397 days of age and from that age onward they presented lower estimates. The nonlinear regression model approach under the mixed-models context allows reduction of residual variance, increasing model accuracy.

The use of palm kernel cake as an alternative to conventional ingredients, due to the presence of residual fat, can also reduce methane emissions. The objective of the study was to evaluate, in two different experiments, the effects of palm kernel cake supplementation on feed intake, enteric methane production and estimates, and the ingestive behavior of buffaloes in the Amazon biome. In experiment 1, to evaluate feed intake, methane production, and feed efficiency, 20 crossbred females, dry and empty, with a mean age of 34 months and an initial body weight of 514 ± 69 kg, were supplemented with palm kernel cake for 60 days. The supply was calculated in relation to body weight (BW) in four treatments: 0% (control); 0.25, 0.50, and 1% of palm kernel cake, distributed in a completely randomized design. In experiment 2, to evaluate the ingestive behavior, 24 mixed-breed, dry, and non-pregnant buffaloes supplemented with palm kernel cake were evaluated in the less rainy season (LR) and the wettest season (WS) of the eastern Amazon, distributed in a completely randomized in the same treatments as experiment 1. The inclusion of palm kernel cake in the supplementation increased the feed intake of dry matter and components (MM, OM, CP, NDF, ADF, and EE) ( P < 0.01), reducing the production of enteric methane intake ( P < 0.01), the ratio per kg of meat produced ( P < 0.01) and feed efficiency ( P < 0.01), and influenced the ingestive behavior (time grazing, rumination, and idleness) during the day. We suggest that further research be carried out to verify the results and improve the use of this co-product as a methanogenesis mitigator., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Amaral Júnior, Martorano, Nahúm, de Castro, Sousa, Rodrigues, da Silva, da Costa Silva, Lourenço Júnior, Berndt and e Silva.)

Sheep farming has grown substantially in the Brazilian Amazon over the previous three decades. This article analyzes the spatial distribution and the dynamics of sheep herd growth using data from the Brazilian Institute of Geography and Statistics-IBGE from 1990 to 2020. The sheep herd growth rates and densities were estimated to assess its dynamics. Maps were then drawn up to show the spatial dynamics of these variables. The highest concentration of herds in the last decade (2010) occurred in Pará, Mato Grosso, and Maranhão states. For each decade there were different growth patterns, but for the entire period (1990 to 2020), there was growth in Mato Grosso, Pará, Maranhão, Tocantins, and Amazonas micro-regions states. The highest density of herd (animals per area) was observed in Maranhão. The potential points for development identified in this study may support strategic planning aimed at strengthening the activity in the region., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2022 Bezerra et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

This study aimed to evaluate the behavior and thermal comfort of 20 Girolando cows (5/8-H/G), with light and dark coats, in the wettest period of the year, in Santarém, Pará, Brazil, in pasture with access to shade, and plenty of drinking water and mineral salt. Animal behavior categories were computed for 12 h a day, on 3 days in a row, by trained observers. Three day shifts were considered: Morning (6:00 a.m. to 9:55 a.m.), Intermediate (10:00 a.m. to 01:55 p.m.) and Afternoon (2:00 p.m. to 05:55 p.m.). The Temperature Index (TI), the Black Globe Humidity Index (BGHI) and the Comfort Index (CI) were calculated to measure thermal comfort. At all times studied, BGHI pointed that the environment was outside the thermal comfort zone. Dark-coated animals spent more 34.26% of the time in activities in the shade. The light-coated animals remained more 11.88% of the time in the sun, performing their natural behaviors. Both light and dark coat animals remained more 77 and 74.44% of the time in the sun, respectively. The behavior "in the sun while grazing" was the most evident, in both coats, in the studied shifts. The behaviors "in the shade while walking" and "in the shade while standing idle" were more evident ( p < 0.01) in dark-coated cattle. The grazing behavior was higher in animals with dark coat ( p < 0.05). In all evaluated shifts, there was a positive correlation between the behavior "in the sun while grazing" with the CI (r = 0.44211; p < 0.0305). Behaviors performed in the shade, such as "idleness while lying down," "ruminating while lying down and standing up," and behaviors "in the sun," "idleness while lying down" and "ruminating while lying down," were negatively correlated with CI. It is concluded that, even in the wettest period of the year, in the Eastern Amazon, Girolando dairy cows are exposed to hot environments, which causes thermal discomfort and changes in their natural behavior, as they spend more time standing in shaded areas, usually in rumination. Also, light-coated cows spend more time in the sun, while dark-coated cows spend more time in the shade. Thus, light-coated cows tend to have health and zootechnical performance negatively affected., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Silva, Silva, Santos, Camargo Junior, Barbosa, Silva, Vinhote, Sousa and Lourenço Júnior.)

Buffaloes are well-adapted to hot, humid climates and muddy terrain, however they show signs of thermal discomfort when exposed to direct sunlight due to their specific structural features such as dark skin, small number of sweat glands/skin area and thick skin. Epidermis layer of the skin, making heat dissipation difficult. The study aimed to evaluate the thermal comfort of female buffaloes raised in traditional and silvopastoral systems, on the island of Marajó, Pará, during the rainy season of the year. The experiment was carried out in Cachoeira do Arari, Retiro Grande, Marajó, Pará (00°55'37.814424"S 48°43'48.143060"W). Twenty female Murrah buffaloes, aged between 2 and 3years and average weight of 282 ± 29 kg, were used. They were fed on pasture and divided into two groups: WS group (with shade) and NS group (without shade). The WS group ( n = 10) remained grazing in a silvopastoral system, with access to the shade of red Jambeiro ( Syzygium malaccense ) trees. The NS group ( n = 10) was kept in grazing, in a traditional system, without access to the shade of trees or shades. The physiological variables rectal temperature (RT), respiratory rate (RR) and body surface temperature (BST) (forehead, left side of the thorax and left flank) were measured at 6:00, 10:00, 14:00, 18:00, and 22:00 h. At the same times, data on air temperature, relative air humidity, wind speed (WSP), solar radiation (SR) and Temperature and Humidity Index (THI) were also recorded. THI, WSP and SR were higher at 14:00 h ( P < 0.05). At 10:00, 14:00, and 18:00 h there was difference of RT between treatments ( P < 0.05), where animals of the NS group had the highest values. In the NS group, the highest value of RT was observed at 14:00 h (39.38 ± 0.43°C), when THI and SR were higher. At 10:00 and 14:00 h, BST was higher in the NS group ( p < 0.05), of 34,55 ± 1.03 and 35.35 ± 1.51°C, respectively, and both groups had the highest value of BST at those same times. There was difference of the RR between treatments at 10:00 h and 14:00 h ( p < 0.05), where the highest values were found in the NS group (33.71 ± 7.58 e 50.40 ± 18.41 mov./min., respectively). Even in the rainy season of the year, the climatic conditions of Ilha do Marajó are unfavorable to the welfare of buffaloes, especially at 2:00 pm, when the SR is higher. Thus, the use of the system with access to shade, such as silvopastoral, is recommended, in order to provide greater thermal comfort to animals and improve their productivity., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Silva, Pantoja, Silva, Almeida, Noronha, Barbosa and Lourenço Júnior.)

To date, no information has been published on the effectiveness of inactivated whole-virus COVID-19 vaccines plus heterologous booster against symptomatic infection and severe outcomes (hospitalization or death) during the dominance of the SARS-CoV-2 Omicron variant period. We evaluated the vaccine effectiveness (VE) of CoronaVac plus BNT162b2 booster during the period of dominance of the Omicron variant in Brazil (January to April 2022). Using a test-negative design, we analysed data for 2,471,576 individuals tested during the Omicron variant's dominant period using a nationally linked database from Brazil. Compared to unvaccinated, vaccinees maintained protection against severe outcomes, with an estimated VE of 84.1% (95% CI:83.2-84.9) at more than 120 days after BNT162b2 booster. Furthermore, while we detected a high level of protection against severe outcomes for individuals up to 79 years old, waning was observed for individuals aged ≥80 years, with VE decreasing from 81.3% (95% CI:77.9-84.2) at 31-60 days to 72.9% (95% CI:70.6-75.1) at 120 days or more after the booster dose. However, no significant protection against symptomatic infection was observed at this time period. In conclusion, except for individuals aged ≥80 years, CoronaVac plus a BNT162b2 booster dose offered high and durable protection against severe outcomes due to Omicron., (© 2022. The Author(s).)

Background: More doses of CoronaVac have been administered worldwide than any other COVID-19 vaccine. However, the effectiveness of COVID-19 inactivated vaccines in pregnant women is still unknown. We estimated the vaccine effectiveness (VE) of CoronaVac against symptomatic and severe COVID-19 in pregnant women in Brazil., Methods: We conducted a test-negative design study in all pregnant women aged 18-49 years with COVID-19-related symptoms in Brazil from March 15, 2021, to October 03, 2021, linking records of negative and positive SARS-CoV-2 reverse transcription polymerase chain reaction (RT-PCR) tests to national vaccination records. We also linked records of test-positive cases with notifications of severe, hospitalised or fatal COVID-19. Using logistic regression, we estimated the adjusted odds ratio and VE against symptomatic COVID-19 and against severe COVID-19 by comparing vaccine status in test-negative subjects to test-positive symptomatic cases and severe cases., Results: Of the 19,838 tested pregnant women, 7424 (37.4%) tested positive for COVID-19 and 588 (7.9%) had severe disease. Only 83% of pregnant women who received the first dose of CoronaVac completed the vaccination scheme. A single dose of the CoronaVac vaccine was not effective at preventing symptomatic COVID-19. The effectiveness of two doses of CoronaVac was 41% (95% CI 27.1-52.2) against symptomatic COVID-19 and 85% (95% CI 59.5-94.8) against severe COVID-19., Conclusions: A complete regimen of CoronaVac in pregnant women was effective in preventing symptomatic COVID-19 and highly effective against severe illness in a setting that combined high disease burden and marked COVID-19-related maternal deaths., (© 2022. The Author(s).)

There is considerable interest in the waning of effectiveness of coronavirus disease 2019 (COVID-19) vaccines and vaccine effectiveness (VE) of booster doses. Using linked national Brazilian databases, we undertook a test-negative design study involving almost 14 million people (~16 million tests) to estimate VE of CoronaVac over time and VE of BNT162b2 booster vaccination against RT-PCR-confirmed severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and severe COVID-19 outcomes (hospitalization or death). Compared with unvaccinated individuals, CoronaVac VE at 14-30 d after the second dose was 55.0% (95% confidence interval (CI): 54.3-55.7) against confirmed infection and 82.1% (95% CI: 81.4-82.8) against severe outcomes. VE decreased to 34.7% (95% CI: 33.1-36.2) against infection and 72.5% (95% CI: 70.9-74.0) against severe outcomes over 180 d after the second dose. A BNT162b2 booster, 6 months after the second dose of CoronaVac, improved VE against infection to 92.7% (95% CI: 91.0-94.0) and VE against severe outcomes to 97.3% (95% CI: 96.1-98.1) 14-30 d after the booster. Compared with younger age groups, individuals 80 years of age or older had lower protection after the second dose but similar protection after the booster. Our findings support a BNT162b2 booster vaccine dose after two doses of CoronaVac, particularly for the elderly., (© 2022. The Author(s).)

The aim of this study was to analyze the influence of distinct production systems and seasonal variation in the Brazilian Eastern Amazon on the meat lipid composition of water buffaloes. Water buffaloes were reared in commercial farms in the Eastern Amazon either in extensive systems (Marajó Island, Nova Timboteua and Santarém locations), during rainy or dry seasons, or intensive (feedlot) systems. Animals reared in extensive systems were fed natural pastures, and those reared in feedlots were fed sorghum silage and commercial pellets. Buffaloes were slaughtered and ribeye muscle ( longissimus lumborum) samples collected. Lipid-soluble antioxidant vitamins and fatty acids were analyzed. The nutritional value of meat from buffaloes reared in Marajó Island extensive system during the rainy season was higher than that of other systems, as it had lower levels of cholesterol and higher amounts of α-tocopherol associated with higher hypocholesterolaemic/hypercholesterolaemic ratio and lower index of atherogenic. Also, this meat had lower percentages of saturated fatty acids and higher proportions of mono- and polyunsaturated fatty acids (PUFA), particularly n-3 PUFA, with increased PUFA/saturated fatty acids ratio and decreased n-6/n-3 PUFA ratio. However, all extensive systems produced meat with a relatively low index of thrombogenicity values, which is advantageous for human health.

Millions of people worldwide are infected with COVID-19, and COVID-19 survivors have been found to suffer from functional disabilities and mental disorders such as depression and anxiety. This is a matter of concern because COVID-19 is still not over. Because reinfection is still possible in COVID-19 survivors, decreased physical function and increased stress and anxiety can lower immune function. However, the optimal exercise intensity and volume appear to remain unknown. Therefore, the current systematic review aimed to evaluate the effect of resistance or aerobic exercises in post-COVID-19 patients after hospital discharge. We conducted searches in the Scopus, SciELO, PubMed, Web of Science, Science Direct, and Google Scholar databases. Studies that met the following criteria were included: (i) English language, (ii) patients with COVID-19 involved with resistance or aerobic exercise programs after hospital discharge. Out of 381 studies reviewed, seven studies met the inclusion criteria. Evidence shows that exercise programs composed of resistance exercise (e.g., 1-2 sets of 8-10 repetitions at 30-80% of 1RM) along with aerobic exercise (e.g., 5 to 30 min at moderate intensity) may improve the functional capacity and quality of life (reduce stress and mental disorders) in post-COVID-19 patients. In addition, only one study reported reinfection of three subjects involved with the exercise program, suggesting that exercise programs may be feasible for the rehabilitation of the patients. A meta-analysis was not conducted because the included studies have methodological heterogeneities, and they did not examine a control group. Consequently, the results should be generalized with caution.

Background: Aging influences COVID-19 severity and response to vaccination, but previous vaccine effectiveness (VE) analyzes lack the power to evaluate its role in subgroups within the elderly age group. Here we analyzed the impact of age on viral vector and inactivated virus vaccines' effectiveness, the main platforms used in low- and middle-income countries., Methods: We report a retrospective longitudinal study of 75,919,840 Brazilian vaccinees from January 18 to July 24, 2021, evaluating documented infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), COVID-19-related hospitalisation, ICU admission, and death. Negative binomial regression models adjusted for sociodemographic characteristics were used for VE estimation., Findings: The overall analyzes of full vaccination showed VE against hospitalisation, ICU admission, and death of 91·4% (95%CI:90·1-92·5), 91·1% (95%CI:88·9-92·9) and 92·3% (95%CI:90·5-93·7) for Vaxzevria and 71·2% (95%CI:70·0-72·4), 72·2% (95%CI:70·2-74·0) and 73·7% (95%CI:72·1-75·2) for CoronaVac, respectively. VE for all outcomes is progressively lower with age. In fully-Vaxzevria-vaccinated individuals aged <60 years, VE against death was 96.5% (95%CI:82.1-99.3) versus 68·5% (95%CI:40·0-83·4) in those ≥90 years. Among fully-CoronaVac-vaccinated individuals, VE against death was 84.8% (95%CI:77.1-89.9) in those <60 years compared to 63.5 (95%CI 58.7-67.7) for vaccinees aged 80-89 years and 48·6%; (95%CI:35·0-59·3) for individuals aged ≥90 years. Post-vaccination daily cumulative incidence curves for all outcomes showed increased risk from younger to elder decades of life. There was no increase in the incidence of hospitalisation for individuals <60 years vaccinated during the same period as those aged ≥90 years., Interpretation: Although both vaccines have been effective in protecting against infection, hospitalization and death; Vaxzevria and CoronaVac demonstrated high effectiveness against severe outcomes for individuals up to 79 years of age. Our results reinforce the idea that booster doses should be carefully considered in elders., Funding: This study was partially supported by a donation from the "Fazer o bem faz bem" program., Competing Interests: VO, VB, MB, and MB-N are employees of Fiocruz, a federal public institution, which manufactures Vaxzevria in Brazil, through a full technology transfer agreement with AstraZeneca. Fiocruz allocates all manufactured products to the Brazilian Ministry of Health for public health service (SUS) use. All other authors report no potential competing interests., (© 2021 The Author(s).)

Introduction: The pandemic caused by the COVID-19 resulted in worldwide social isolation and leading to significant personal distress, particularly among health professionals on the front lines. Those factors' relevance and their impact on sexual function in this population have not yet been established., Aim: To evaluate the impact of the pandemic on sexual function in healthcare professionals and medical students at a reference center in the treatment of COVID-19 in Brazil., Methods: A cross-sectional analysis with online questionnaires about sexual function was sent to health professionals and medical students from the HC-FMUSP medical complex. The questionnaire evaluated Total Sexual and Masturbatory Frequency prior and during the pandemic, libido and sexual satisfaction changes with a detailed inquire about demographics and personal factors. An objective assessment of sexual function was also made using the validated sexual quotient questionnaires., Main Outcome Measures: Differences in intercourse frequency, libido, and overall sexual satisfaction, in a sample of healthcare professionals particularly vulnerable to the pandemic effects., Results: A total of 1,314 responses were available with a mean age of 37 years. Worsening of sexual satisfaction was reported by 44.5% of the participants, with the following associated factors: Lower libido, missing Nightlife, Higher Masturbatory Frequency, and isolation from the partner. Remaning sexualy actively and having higher sexual frequency appear to decrease the chance of worsening sexual function. Worsening of Libido was reported by 37% and had several associated factors, including missing of Nightlife, older age, isolation from the partner among others. Being male and sexually active was associated with a smaller chance of reporting lower libido., Conclusion: We were able to observe a sharp drop in Libido and General Sexual Satisfaction. Although an increase in pornography consumption and masturbatory frequency did occur, these factors were not associated with greater sexual satisfaction. The impact of COVID-19 on this population's sexual health is not to be underestimated and should be further studied in the follow-up of the pandemic. Neto RP, Nascimento BCG, Carvalho dos Anjos Silva G, et al. Impact of COVID-19 Pandemic on the Sexual Function of Health Professionals From an Epicenter in Brazil. Sex Med 2021;9:100408., (Copyright © 2021 International Society for Sexual Medicine. Published by Elsevier Inc. All rights reserved.)

Various studies have identified that between 2012 and 2017, Brazil's semiarid region suffered severe drought. However, few studies have analyzed whether this drought also affected the eastern coastal region of Northeast Brazil (ENEB). Therefore, the objective of this work is to identify rainfall anomalies in these regions and verify the hydrometeorological impact on reservoirs in the 2012-2017 interval. For this purpose, we used precipitation data and atmospheric variables in the period from 1981 to 2017 to investigate the rainy season and associated dynamic patterns, as well as the consequences of these mechanisms on the variation of the water parameters of important reservoirs. The results indicated that rain events in the ENEB during 2012-2017 presented similar climatological behavior, without the characteristic of a drought event as observed in the semiarid region. The meteorological analyses showed that the combination of convergence with moisture over the Atlantic Ocean possibly favored greater frequency of shallow convective rainfall in ENEB, an important factor to explain the absence of generalized negative anomalies in the region. As a consequence, the reservoirs did not suffer from water collapse, unlike in the semiarid region.

The objective was to evaluate the effect of concentrate supplementation using by-products of the Amazonian industry on milk production, milk composition, and milk fatty acid profile of dairy buffaloes. Twelve lactating buffaloes (544.5 ± 35.6 kg, 6.4 ± 2.2 years old, 59 ± 6 days in milk) were allotted in a pasture of Mombaça grass and managed under rotational grazing (4 days occupancy/28 days rest). A 3 × 3 Latin square was adopted, and each animal alternately received three supplementary treatments based on corn bran + soybean meal or cupuaçu cake or murumuru cake for 21 days per treatment. Murumuru cake increased the levels of lauric acid and myristic acid in the milk (p < 0.05). Murumuru cake reduced the unsaturated fatty acid contents in the milk compared with animals fed control diet or cupuaçu cake (24.27% vs. 25.24% vs. 25.08%). The n-6/n-3 ratio was 2.6, 1.97, and 2.0 in the control, cupuaçu, and murumuru groups, respectively. Based on this parameter, cakes made from cupuaçu as well as murumuru could be considered to be adequate for inclusion in dairy water buffalo feed. However, the murumuru cake addition requires some caution because its use induces the secretion of higher levels of lauric and myristic fatty acids that are related to human cardiovascular disease., (© 2021 Japanese Society of Animal Science.)

Introduction: Carbapenemase-resistant enterobacteria that produce the bla NDM gene are found worldwide. However, this is the first report of blaNDM in Klebsiella aerogenes in Brazil., Methods: The identification of bacterial species was performed using anautomated system and confirmed by biochemical tests, 16S rRNA gene sequencing, and detection of resistance genes., Results: The clinical isolate showed minimum inhibitory concentration resistance to meropenem and polymyxin B at 8mg/L and 4mg/L, respectively. Only the blaNDM gene was detected., Conclusions: The current report of the blaNDM gene in isolated MDR enterobacteria indicates that this gene can spread silently in a hospital setting.

This study aimed to assess the effect of thermal-hydraulic variables in female buffaloes with or without direct solar exposure in a year of strong El Niño through behavior responses and infrared thermography to reinforce the environmental comfort indicators, in Marajó Island, Pará, Brazil. The experiment was carried out in Cachoeira do Arari municipality and 20 female Murrah buffaloes were randomly assigned to two groups: Group WS ( n = 10) was kept in pickets with native trees. Group NS ( n = 10) was kept in crush squeeze with no shade. Data on air temperature (AT, °C), relative air humidity (RH, %), wind velocity (WV, m/s), rectal temperature (RT), respiratory rate (RR), and body surface temperature (BST) were collected. Practical Buffalo Comfort Climatic Condition Index (BCCCI), practical Buffalo Environmental Comfort Index (BECI), Temperature and Humidity Index (THI) and Benezra's Thermal Comfort Index (BTCI) were obtained. Infrared thermography analysis was carried out with a FLIR T-series T640bx camera. Data on time spent grazing, ruminating, idleness, and in other activities were recorded. A significant difference in AT of ~1°C was found between the groups at 6 a.m., 10 a.m. and 6 p.m. THI indicated emergency conditions. Female buffaloes were at danger PBCCCI conditions at 2 p.m. There was also significant difference for RT between treatments at 10 a.m., 2 p.m. and 6 p.m., whose values were higher ( P < 0.05) for animals from NS Group, with the highest mean time at 2 p.m. Pearson correlation was significant and positive ( P < 0.01) between RT mean and VUL, TI and ORB mean, maximum and minimum temperatures. The total time given to grazing was 518.2 min for the group NS and 629.5 min for the group WS. Rumination was more pronounced in the afternoon shift for the group NS. Buffaloes kept in a system with trees graze, ruminate and perform other activities with more intensity than animals raised in systems without access to shade, and tend to hyperthermia, mainly at 10 a.m. and 2 p.m., in Marajó Island, Pará, Brazil., (Copyright © 2020 Athaíde, Joset, de Almeida, Pantoja, Noronha, Bezerra, Barbosa, Martorano, da Silva and Lourenço Júnior.)