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Measurement(s) androgen receptor signaling pathway Technology Type(s) Next Generation Sequencing Sample Characteristic - Organism Homo

Wasabi (Wasabia japonica Matsumura) is a perennial plant, and its rhizome is widely used as a pungent spice. Wasabi rhizome has been reported to have various physiological activities, but the flower has not been studied in depth. The aim of the present study was to isolate compounds from wasabi flowers and clarify their antioxidant and anti-inflammatory activities. Three phenylpropanoids, one alkaloid and seven flavonoids were isolated from wasabi flowers. Among them, 2"-O-trans sinapoyl isovitexin was identified as a novel compound. Five compounds inhibited 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity, with IC50 values of less than 100 µM. Luteolin isolated in this study was found to inhibit nitric oxide production in macrophage-like J774.1 cells, with an IC50 value of 24.1 ± 4.4 µM. Our results indicate that the phenolic compounds in wasabi flowers are effective ingredients for antioxidant and anti-inflammatory activities. For these reasons, wasabi flowers are expected to be used effectively as a functional food. [ABSTRACT FROM AUTHOR]

Wasabi is a member of the Brassicaceae family and produces various isothiocyanates (ITCs). Because 6-methylsulfinylhexyl ITC (6-MSITC) is stable among wasabi ITCs, it has potential as a functional compound. Hair loss can have psychological and social effects on an individual because of its impact on appearance. In this study, we investigated the stimulatory effects of 6-MSITC by culturing on human follicle dermal papilla cells (DPCs). Results showed that 6-MSITC significantly promoted DPC proliferation and upregulated vascular endothelial growth factor (VEGF) mRNA levels compared to control. Furthermore, 6-MSITC significantly upregulated adenosine A2b receptor (ADORA2b) mRNA levels. Previous studies have reported that VEGF was induced through ADORA1 and ADORA2 pathways. We suggest that 6-MSITC stimulates hair growth on DPCs related to the upregulation of ADORA2b mRNA level. Thus, 6-MSITC may be used as a functional compound. [ABSTRACT FROM AUTHOR]

Wasabi is a plant of Japanese origin. It belongs to the Brassicaceae family and produces various isothiocyanates (ITCs). Because 6-methylsulfinylhexyl isothiocyanate (6-MSITC) is particularly stable among wasabi ITCs, it has potential as a functional compound. To clarify the antioxidative activity of 6-MSITC, we investigated its inhibitory effect on superoxide anion ( O2·-)generation from phorbol 12-myristate 13-acetate (PMA)-stimulated differentiated HL-60 human promyelocytic leukemia cells. Our results showed that 6-MSITC inhibited O2·- generation in these cells (IC50 value: 28.9 μM). 6-MSITC at 0.8, 4, and 20 μM significantly inhibited the consumption of exogenously added NADH compared with the control. On the other hand, < 263 μM 6-MSITC did not scavenge O2·- in cell-free experiments. Therefore, we suggest that 6-MSITC inhibited O2·- generation by decreasing an enzymatic reaction with NADPH oxidase. Thus, 6-MSITC may be useful as a functional compound. [ABSTRACT FROM AUTHOR]

Wasabi is a plant of Japanese origin belonging to the Brassicaceae family. Although the wasabi rhizome is a popular condiment in Japan, the leaf is typically discarded. For utilization of the wasabi leaf, we investigated its antiobesity effect on Wistar rats fed a high-fat diet containing wasabi leaf extract (WLE) prepared with 50% ethanol. At the experimental endpoint, WLE had significantly decreased the body weight of rats and upregulated the mRNA expression of the β3-adrenergic receptor (Adrb3) in the interscapular brown adipose tissue (BAT). WLE may have promoted lipid absorption from the dietary fat, as the fecal lipid content was considerably lower in the WLE group. These results suggest that WLE suppressed obesity in rats fed a high-fat diet, which was related to the upregulation of Adrb3 mRNA expression in the interscapular BAT without increased fecal lipid excretion. Thus, wasabi leaves may be used as a functional food material for the suppression of obesity. [ABSTRACT FROM AUTHOR]

Wasabi (Japanese horseradish, Eutrema japonicum) is the only cultivated species in the genus Eutrema with functional components that provide a strong pungent flavor. To evaluate genetic resources for wasabi breed‐ ing, we surveyed variations in the two most abundant isothiocyanate (ITC) components in wasabi, allyl iso‐ thiocyanate (AITC) and 6-methylsulfinyl (hexyl) isothiocyanate (6-MSITC, hexaraphane). We also examined the phylogenetic relationships among 36 accessions of wild and cultivated wasabi in Japan using chloroplast DNA analysis. Our results showed that (i) the 6-MSITC content in currently cultivated wasabi accessions was significantly higher than in escaped cultivars, whereas the AITC content was not significantly different. (ii) Additionally, the 6-MSITC content in cultivated wasabi was significantly lower in the spring than during other seasons. This result suggested that the 6-MSITC content responds to environmental conditions. (iii) The phylogenetic position and the 6-MSITC content of accessions from Rebun, Hokkaido Prefecture had different profiles compared with those from southern Honshu, Japan, indicating heterogeneity of the Rebun popula‐ tions from other Japanese wasabi accessions. (iv) The total content of AITC and 6-MSITC in cultivated wasabi was significantly higher than that of wild wasabi. In conclusion, old cultivars or landraces of wasabi, “zairai”, are the most suitable candidates for immediate use as genetic resources. [ABSTRACT FROM AUTHOR]