1. Analysis of nucleosides and nucleotides in infant formula by liquid chromatography-tandem mass spectrometry.
- Author
-
Gill BD, Indyk HE, and Manley-Harris M
- Subjects
- Allergens chemistry, Animals, Cattle, Goats, Humans, Infant, Milk chemistry, Sensitivity and Specificity, Soy Milk chemistry, Chromatography, Liquid methods, Food Analysis methods, Infant Formula chemistry, Nucleosides chemistry, Nucleotides chemistry, Tandem Mass Spectrometry methods
- Abstract
A method for the simultaneous analysis of nucleosides and nucleotides in infant formula using reversed-phase liquid chromatography-tandem mass spectrometry is described. This approach is advantageous for compliance testing of infant formula over other LC-MS methods in which only nucleotides or nucleosides are measured. Following sample dissolution, protein was removed by centrifugal ultrafiltration. Chromatographic analyses were performed using a C18 stationary phase and gradient elution of an ammonium acetate/bicarbonate buffer, mass spectrometric detection and quantitation by a stable isotope-labelled internal standard technique. A single laboratory validation was performed, with spike recoveries of 80.1-112.9% and repeatability relative standard deviations of 1.9-7.2%. Accuracy as bias was demonstrated against reference values for NIST1849a certified reference material. The method has been validated for the analysis of bovine milk-based, soy-based, caprine milk-based and hydrolysed milk protein-based infant formulae.
- Published
- 2013
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