360 results on '"H. Sugimura"'
Search Results
2. Coping strategies and risk of cardiovascular disease incidence and mortality: the Japan Public Health Center-based prospective Study
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Thomas, Svensson, Manami, Inoue, Norie, Sawada, Kazumasa, Yamagishi, Hadrien, Charvat, Isao, Saito, Yoshihiro, Kokubo, Hiroyasu, Iso, Noriyuki, Kawamura, Kenji, Shibuya, Masaru, Mimura, Shoichiro, Tsugane, S, Tsugane, N, Sawada, M, Iwasaki, S, Sasazuki, T, Shimazu, T, Yamaji, T, Hanaoka, J, Ogata, S, Baba, T, Mannami, A, Okayama, Y, Kokubo, K, Miyakawa, F, Saito, A, Koizumi, Y, Sano, I, Hashimoto, T, Ikuta, Y, Tanaba, H, Sato, Y, Roppongi, T, Takashima, Y, Miyajima, N, Suzuki, S, Nagasawa, Y, Furusugi, N, Nagai, Y, Ito, S, Komatsu, T, Minamizono, H, Sanada, Y, Hatayama, F, Kobayashi, H, Uchino, Y, Shirai, T, Kondo, R, Sasaki, Y, Watanabe, Y, Miyagawa, Y, Kobayashi, M, Machida, K, Kobayashi, M, Tsukada, Y, Kishimoto, E, Takara, T, Fukuyama, M, Kinjo, M, Irei, H, Sakiyama, K, Imoto, H, Yazawa, T, Seo, A, Seiko, F, Ito, F, Shoji, R, Saito, A, Murata, K, Minato, K, Motegi, T, Fujieda, S, Yamato, K, Matsui, T, Abe, M, Katagiri, M, Suzuki, M, Doi, A, Terao, Y, Ishikawa, T, Tagami, H, Sueta, H, Doi, M, Urata, N, Okamoto, F, Ide, H, Goto, N, Onga, H, Takaesu, M, Uehara, T, Nakasone, M, Yamakawa, F, Horii, I, Asano, H, Yamaguchi, K, Aoki, S, Maruyama, M, Ichii, M, Takano, Y, Tsubono, K, Suzuki, Y, Honda, K, Yamagishi, S, Sakurai, N, Tsuchiya, M, Kabuto, M, Yamaguchi, Y, Matsumura, S, Sasaki, S, Watanabe, M, Akabane, T, Kadowaki, M, Inoue, M, Noda, T, Mizoue, Y, Kawaguchi, Y, Takashima, Y, Yoshida, K, Nakamura, R, Takachi, J, Ishihara, S, Matsushima, S, Natsukawa, H, Shimizu, H, Sugimura, S, Tominaga, N, Hamajima, H, Iso, T, Sobue, M, Iida, W, Ajiki, A, Ioka, S, Sato, E, Maruyama, M, Konishi, K, Okada, I, Saito, N, Yasuda, S, Kono, and S, Akiba
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Male ,Coping (psychology) ,medicine.medical_specialty ,Myocardial Infarction ,Kaplan-Meier Estimate ,030204 cardiovascular system & hematology ,03 medical and health sciences ,0302 clinical medicine ,Japan ,Risk Factors ,Internal medicine ,Adaptation, Psychological ,medicine ,Humans ,Prospective Studies ,030212 general & internal medicine ,Prospective cohort study ,Stroke ,Aged ,Proportional hazards model ,business.industry ,Incidence ,Incidence (epidemiology) ,Hazard ratio ,Avoidance coping ,Middle Aged ,medicine.disease ,Cardiovascular Diseases ,Cohort ,Physical therapy ,Female ,Cardiology and Cardiovascular Medicine ,business - Abstract
Aims Coping strategies may be significantly associated with health outcomes. This is the first study to investigate the association between baseline coping strategies and cardiovascular disease (CVD) incidence and mortality in a general population cohort. Methods and results The Japan Public Health Center-based prospective Study asked questions on coping in its third follow-up survey (2000–04). Analyses on CVD incidence and mortality included 57 017 subjects aged 50–79 without a history of CVD and who provided complete answers on approach- and avoidance-oriented coping behaviours and strategies. Cox regression models, adjusted for confounders, were used to determine hazard ratios (HRs) according to coping style. Mean follow-up time was 7.9 years for incidence and 8.0 years for mortality. The premorbid use of an approach-oriented coping strategy was inversely associated with incidence of stroke (HR = 0.85; 95% CI, 0.73–1.00) and CVD mortality (HR = 0.74; 95% CI, 0.55–0.99). Stroke subtype analyses revealed an inverse association between the approach-oriented coping strategy and incidence of ischaemic stroke (HR = 0.79; 95% CI, 0.64–0.98) and a positive association between the combined coping strategy and incidence of intra-parenchymal haemorrhage (HR = 2.03; 95% CI, 1.01–4.10). Utilizing an avoidance coping strategy was associated with increased mortality from ischaemic heart disease (IHD) only in hypertensive individuals (HR = 3.46; 95% CI, 1.07–11.18). The coping behaviours fantasizing and positive reappraisal were associated with increased risk of CVD incidence (HR = 1.24; 95% CI, 1.03–1.50) and reduced risk of IHD mortality (HR = 0.63; 95% CI, 0.40–0.99), respectively. Conclusion An approach-oriented coping strategy, i.e. proactively dealing with sources of stress, may be associated with significantly reduced stroke incidence and CVD mortality in a Japanese population-based cohort.
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- 2016
3. Modification of the Excess Risk of Coronary Heart Disease Due to Smoking by Seafood/Fish Intake
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E. S. Eshak, H. Iso, K. Yamagishi, Y. Kokubo, I. Saito, H. Yatsuya, N. Sawada, M. Inoue, S. Tsugane, T. Sobue, T. Hanaoka, J. Ogata, S. Baba, T. Mannami, A. Okayama, K. Miyakawa, F. Saito, A. Koizumi, Y. Sano, I. Hashimoto, T. Ikuta, Y. Miyajima, N. Suzuki, S. Nagasawa, Y. Furusugi, N. Nagai, H. Sanada, Y. Hatayama, F. Kobayashi, H. Uchino, Y. Shirai, T. Kondo, R. Sasaki, Y. Watanabe, Y. Miyagawa, Y. Kobayashi, Y. Kishimoto, E. Takara, T. Fukuyama, M. Kinjo, M. Irei, H. Sakiyama, K. Imoto, H. Yazawa, T. Seo, A. Seiko, F. Ito, F. Shoji, A. Murata, K. Minato, K. Motegi, T. Fujieda, K. Matsui, T. Abe, M. Katagiri, M. Suzuki, M. Doi, A. Terao, Y. Ishikawa, T. Tagami, H. Sueta, H. Doi, M. Urata, N. Okamoto, F. Ide, N. Onga, H. Takaesu, M. Uehara, F. Horii, I. Asano, H. Yamaguchi, K. Aoki, S. Maruyama, M. Ichii, M. Takano, Y. Tsubono, K. Suzuki, Y. Honda, S. Sakurai, M. Kabuto, M. Yamaguchi, Y. Matsumura, S. Sasaki, S. Watanabe, M. Akabane, T. Kadowaki, M. Noda, Y. Kawaguchi, Y. Takashima, K. Nakamura, S. Matsushima, S. Natsukawa, H. Shimizu, H. Sugimura, S. Tominaga, M. Iida, W. Ajiki, A. Ioka, S. Sato, E. Maruyama, M. Konishi, K. Okada, N. Yasuda, and S. Kono
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Male ,medicine.medical_specialty ,Time Factors ,Epidemiology ,Health Behavior ,Protective factor ,Coronary Disease ,Sex Factors ,Japan ,Internal medicine ,Environmental health ,Humans ,Medicine ,Fish intake ,Myocardial infarction ,Risk factor ,Aged ,Dose-Response Relationship, Drug ,business.industry ,Smoking ,Hazard ratio ,Age Factors ,Absolute risk reduction ,food and beverages ,Middle Aged ,medicine.disease ,Confidence interval ,Coronary heart disease ,Diet ,Seafood ,Cardiovascular Diseases ,Cardiology ,Female ,business - Abstract
Seafood/fish intake has been regarded as a protective factor for coronary heart disease (CHD), while smoking is a strong risk factor. To examine whether associations between smoking and risk of CHD are modified by seafood/fish intake, we studied 72,012 Japanese men and women aged 45-74 years who completed 2 food frequency questionnaires, 5 years apart, during the period 1995-2009. After 878,163 person-years of follow-up, 584 incident cases of CHD (101 fatal and 483 nonfatal), including 516 myocardial infarctions, were documented. There was a clear dose-response association between smoking and CHD risk among subjects with a low seafood/fish intake (
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- 2014
4. Hypernuclear spectroscopy of products from Li-6 projectiles on a carbon target at 2 A GeV
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Patrick Achenbach, L. Nungesser, T. Hiraiwa, G.J. Tambave, Hiroyuki Tamura, C.J. Yoon, S. Voltz, J. Pochodzalla, T.R. Saito, W. Ott, G. Ickert, Yutaka Mizoi, S. Minami, Tomofumi Nagae, C. Rappold, M. Sekimoto, B. Özel-Tashenov, J. Hoffmann, A. Okamura, Tomokazu Fukuda, S. Bianchin, H. Simon, S. Erturk, M. Sako, T. Takahashi, Atsushi Sakaguchi, B. Göküzüm, S. Ajimura, C. Caesar, S. J. Kim, C. Ayerbe Gayoso, A. Le Fèvre, M. Moritsu, E. Guliev, N. Yokota, M. Kavatsyuk, Z.S. Ketenci, K. Koch, N. Kurz, C.J. Schmidt, Minu Kim, K. Yoshida, V. Bozkurt, O. Bertini, D. Nakajima, T. Aumann, Y. Ma, E. H. Kim, D. Khaneft, H. C. Bhang, F. E. Maas, H. Sugimura, W. Trautmann, KVI - Center for Advanced Radiation Technology, Mizoi, Yutaka -- 0000-0002-4749-0815, Ayerbe Gayoso, Carlos -- 0000-0001-8640-5380, Rappold, Christophe -- 0000-0002-5390-5052, Achenbach, Patrick -- 0000-0002-6754-653X, Caesar, Christoph -- 0000-0001-5844-7073, [Rappold, C.] Univ Giessen, D-35392 Giessen, Germany -- [Rappold, C. -- Kim, E. -- Nakajima, D. -- Saito, T. R. -- Bertini, O. -- Bianchin, S. -- Bozkurt, V. -- Ma, Y. -- Maas, F. -- Minami, S. -- Oezel-Tashenov, B. -- Yoshida, K. -- Aumann, T. -- Gokuzum, B. -- Hoffmann, J. -- Ickert, G. -- Khaneft, D. -- Koch, K. -- Kurz, N. -- Le Fevre, A. -- Ott, W. -- Schmidt, C. J. -- Simon, H. -- Trautmann, W. -- Voltz, S.] GSI Helmholtz Ctr Heavy Ion Res, D-64291 Darmstadt, Germany -- [Kim, E. -- Bhang, H. C. -- Kim, M. -- Kim, S. -- Yoon, C. J.] Seoul Natl Univ, Seoul 151747, South Korea -- [Nakajima, D.] Univ Tokyo, Bunkyo Ku, Tokyo 1130033, Japan -- [Saito, T. R. -- Bertini, O. -- Ma, Y. -- Maas, F. -- Achenbach, P. -- Gayoso, C. Ayerbe -- Khaneft, D. -- Nungesser, L. -- Pochodzalla, J.] Johannes Gutenberg Univ Mainz, D-55099 Mainz, Germany -- [Saito, T. R. -- Maas, F. -- Yoshida, K.] HIM, D-55099 Mainz, Germany -- [Bozkurt, V. -- Erturk, S. -- Gokuzum, B. -- Ketenci, Z. S.] Nigde Univ, TR-51100 Nigde, Turkey -- [Kavatsyuk, M. -- Guliev, E. -- Tambave, G. J.] Univ Groningen, KVI, NL-9747 AA Groningen, Netherlands -- [Yoshida, K. -- Sakaguchi, A.] Osaka Univ, Toyonaka, Osaka 5600043, Japan -- [Ajimura, S.] RCNP, Osaka 5670047, Japan -- [Aumann, T. -- Caesar, C.] Tech Univ Darmstadt, D-64289 Darmstadt, Germany -- [Fukuda, T. -- Mizoi, Y.] Osaka Electrocommun Univ, Neyagawa, Osaka 5728530, Japan -- [Hiraiwa, T. -- Moritsu, M. -- Nagae, T. -- Okamura, A. -- Sako, M. -- Sugimura, H. -- Yokota, N.] Kyoto Univ, Kyoto 6068502, Japan -- [Le Fevre, A.] SUBATECH, F-44307 Nantes 3, France -- [Sekimoto, M. -- Takahashi, T.] KEK, Tsukuba, Ibaraki 3050801, Japan -- [Tamura, H.] Tohoku Univ, Aoba Ku, Sendai, Miyagi 9807875, Japan, and 0-Belirlenecek
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Nuclear reaction ,(Li-6 ,E=2 GeV/nucleon ,Nuclear and High Energy Physics ,Maximum likelihood ,WEAK DECAY ,FOS: Physical sciences ,LIFETIME ,T-1/2. Compared with other data ,deduced Lambda mass ,C-12(LAMBDA) ,H-4) ,T-1/2 ,measured Lambda, H-3,H-4 Lambda-hypernuclei invariant mass distribution, T-1/2 ,measured Lambda ,Invariant mass ,Nuclear Experiment (nucl-ex) ,LIGHT HYPERFRAGMENTS ,Spectroscopy ,Nuclear Experiment ,X) ,Physics ,H-4 Lambda-hypernuclei invariant mass distribution ,H-3 ,Projectile ,Significance values ,lifetime measurement ,deduced Lambda mass, H-3,H-4 Lambda-hypernuclei mass, T-1/2. Compared with other data, lifetime measurement ,NUCLEAR REACTIONS C(Li-6 ,Decay time ,LAMBDA-HYPERNUCLEI ,Atomic physics ,t) ,NUCLEAR REACTIONS C(Li-6, X), (Li-6, t), (Li-6, H-4), E=2 GeV/nucleon ,H-4 Lambda-hypernuclei mass ,Hypertriton ,RELATIVISTIC HYPERNUCLEI - Abstract
WOS: 000322848900009, A novel experiment, aiming at demonstrating the feasibility of hypernuclear spectroscopy with heavy ion beams, was conducted. Using the invariant mass method, the spectroscopy of hypernuclear products of Li-6 projectiles on a carbon target at 2 A GeV was performed. Signals of the Lambda-hyperon and H-3(Lambda) and H-4(Lambda) hypernuclei were observed for final states of p + pi(-), He-3 + pi(-) and He-4 + pi(-), respectively, with significance values of 6.7, 4.7 and 4.9 sigma. By analyzing the proper decay time from secondary vertex distribution with the unbinned maximum likelihood fitting method, their lifetime values were deduced to be 262(-43)(+56) +/- 45 ps for Lambda, 183(-32)(+42) +/- 37 ps for H-3(Lambda), and 140(-33)(+48) +/- 35 ps for H-4(Lambda). (c) 2013 Elsevier B.V. All rights reserved., Electronics Department of the Institute for Nuclear Physics of Mainz University; Helmholtz association as Helmholtz-University Young Investigators Group at GSI [VH-NG-239]; German Research Foundation (DFG) [SA 1696/1-1]; Ministry of Education, Science and Culture of Japan [18042008]; EU FP7 Hadron-Physics-2 SPHERE; GSI Department of Accelerator; GSI Department of Experimental Electronics; GSI Department of the Detector Laboratory; GSI Department of the Target Laboratory, The authors would like to thank the GSI Departments of Accelerator, of Experimental Electronics, of the Detector Laboratory and of the Target Laboratory and the Electronics Department of the Institute for Nuclear Physics of Mainz University for supporting the project. The HypHI project is funded by the Helmholtz association as Helmholtz-University Young Investigators Group VH-NG-239 at GSI, and the German Research Foundation (DFG) under contract number SA 1696/1-1. The authors acknowledge the financial support provided by the Ministry of Education, Science and Culture of Japan, Grant-in-Aid for Scientific Research on Priority Areas 449, and Grant-in-Aid for promotion of Cooperative Research in Osaka Electro-Communication University (2004-2006). This work is also supported by the Ministry of Education, Science and Culture of Japan, Grants-in-Aid for Scientific Research 18042008 and EU FP7 Hadron-Physics-2 SPHERE. A part of this work was carried out on the HIMSTER high performance computing infrastructure provided by the Helmholtz-Institute Mainz.
- Published
- 2013
5. Alloying Behavior of Quaternary Elements in Ni3(Si,Ti)
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Yasuyuki Kaneno, H. Sugimura, and Takayuki Takasugi
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Materials science ,Mechanical Engineering ,Condensed Matter Physics ,Standard enthalpy of formation ,Thermodynamic model ,chemistry.chemical_compound ,Crystallography ,chemistry ,Mechanics of Materials ,Ternary compound ,Atom ,Site occupancy ,General Materials Science ,Solubility ,Quaternary - Abstract
The thermodynamic treatment, which is based on the difference of heat of formation among three extreme cases for the substitution of a quaternary element X, was applied to predict the substitution behavior of quaternary elements in Ni3(Si,Ti). The heat of formation of a hypothetical ternary compound Ni6SiTi in which X elements substitute for relevant sites was calculated by using a geometric model based on an extended Miedema’s theory. According to the prediction, Cr and W (6), Mn and Re (7), Fe and Os (8), Co and Ir (9), Pt (10), and Cu and Au (11) substitute for Ni atoms. Ge (14) substitutes for Si atoms. Zr and Hf (4), V, Nb, and Ta (5), Mo (6), and Al (13) substitute for Ti atoms. Ga (13) substitutes for Si or Ti atom. The prediction for Ta is consistent with the reported experimental result. Also, the solubility limits of quaternary elements for the Ti site in Ni3(Si,Ti) are ranked in the sequence of Ta > Nb > V > Hf > Zr, and correlated with the size misfit parameter between Ti and the quaternary element X, and the difference in heats of formation between Ni6SiTi and Ni6SiX.
- Published
- 2011
6. Alloying Behavior of Ni3M-Type Compounds with D0a Structure
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Yasuyuki Kaneno, Takayuki Takasugi, and H. Sugimura
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Materials science ,Mechanical Engineering ,Substitution (logic) ,Type (model theory) ,Condensed Matter Physics ,Standard enthalpy of formation ,Weak binding ,Thermodynamic model ,Crystallography ,Mechanics of Materials ,Phase (matter) ,General Materials Science ,Ternary phase diagram ,Ternary operation - Abstract
The atom substitution preference of ternary additions in Ni3M-type GCP (geometrically close-packed) compounds with D0a structure was determined from the direction of single-phase region of the GCP phase on the reported ternary phase diagrams. In Ni3Nb, Co and Cu preferred the substitution for Ni-site, Hf, Ta, Ti, V and W the substitution for Nb-site, and Fe the substitution for both sites. In Ni3Ta, Co, Cu, Fe, Ir, Mn and Re preferred the substitution for Ni-site, Mo and Nb the substitution for Ta-site, and Al and Cr the substitution for both sites. In Ni3Mo, Pd and W preferred the substitution for Ni-site, and Al, Nb, Ta, Ti and Zr the substitution for Mo-site. The thermodynamic model, which was based on the change in heat of formation of the host compound by a small addition of ternary solute, was applied to predict the atom substitution preference of ternary additions. The heat of compound formation used in the thermodynamic calculation was derived from Miedema’s formula. Good agreement was obtained between the thermodynamic model and the result of the literature search. For Ni3Mo, with a small negative heat of formation, a weak binding force between the constituent elements is often enhanced by the addition of the ternary elements that substitute for Mo-site.
- Published
- 2011
7. Alloying Behavior of Ni3Nb
- Author
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Yasuyuki Kaneno, Takayuki Takasugi, and H. Sugimura
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Work (thermodynamics) ,Materials science ,Mechanical Engineering ,Substitution (logic) ,Thermodynamics ,Condensed Matter Physics ,k-nearest neighbors algorithm ,Thermodynamic model ,Crystallography ,Mechanics of Materials ,General Materials Science ,Ternary phase diagram ,Solubility ,Bond energy ,Ternary operation - Abstract
The site preference of ternary additions in Ni 3 Nb (D0 a ) was determined from the direction of solubility lobes of the GCP phases which were available from the reported ternary phase diagrams and constructed in the present work. It was shown that Cr, Co and Cu preferred the substitution for Ni-site, Ti, V, Hf, Ta and W the substitution for Nb-site, and Fe the substitution for both sites. The thermodynamic model, which was based on the change in total bonding energy of the host compound by a small addition of ternary solute, was applied to predict the site preference of ternary additions. The bond energy of each nearest neighbor pair used in the thermodynamic calculation was derived from the heat of compound formation by Miedema's formula. The agreement between the thermodynamic model and the experimental result was excellent. From both experimental and theoretical results, both the transition and B-subgroup elements have two possibilities, i.e., the case of substitution for Ni-site or the case of substitution for Nb-site, depending on the relative value of two interaction energies.
- Published
- 2010
8. Nationwide surveillance of bacterial respiratory pathogens conducted by the Japanese Society of Chemotherapy in 2007: general view of the pathogens’ antibacterial susceptibility
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Y. Niki, T. Matsumoto, S. Kohno, N. Aoki, A. Watanabe, J. Sato, R. Hattori, M. Terada, N. Koashi, T. Kozuki, A. Maruo, K. Morita, K. Ogasawara, Y. Takahashi, J. Watanabe, K. Sunakawa, K. Totsuka, H. Hanaki, M. Yagisawa, K. Takeuchi, S. Fujimura, H. Takeda, H. Ikeda, N. Sato, K. Niitsuma, M. Saito, S. Koshiba, M. Kaneko, M. Miki, S. Nakanowatari, Y. Honda, J. Chiba, H. Takahashi, M. Utagawa, T. Kondo, A. Kawana, H. Konosaki, Y. Aoki, H. Ueda, H. Sugiura, M. Ichioka, H. Goto, D. Kurai, M. Okazaki, K. Yoshida, T. Yoshida, Y. Tanabe, S. Kobayashi, M. Okada, H. Tsukada, Y. Imai, Y. Honma, K. Nishikawa, T. Yamamoto, A. Kawai, T. Kashiwabara, Y. Takesue, Y. Wada, K. Nakajima, H. Toda, N. Mitsuno, H. Sugimura, S. Yoshioka, M. Kurokawa, Y. Munekawa, H. Nakajima, S. Kubo, Y. Ohta, K. Mikasa, K. Maeda, K. Kasahara, A. Koizumi, R. Sano, S. Yagi, M. Takaya, Y. Kurokawa, N. Kusano, E. Mihara, M. Kuwabara, Y. Fujiue, T. Ishimaru, N. Matsubara, Y. Kawasaki, H. Tokuyasu, K. Masui, K. Negayama, N. Ueda, M. Ishimaru, Y. Nakanishi, M. Fujita, J. Honda, J. Kadota, K. Hiramatsu, Z. Nagasawa, M. Suga, H. Muranaka, K. Yanagihara, J. Fujita, and M. Tateyama
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Adult ,Microbiology (medical) ,Imipenem ,Respiratory tract infection ,Klebsiella pneumoniae ,Resistance ,Microbial Sensitivity Tests ,Biology ,medicine.disease_cause ,Gram-Positive Bacteria ,Microbiology ,Haemophilus influenzae ,Moraxella catarrhalis ,Japan ,Ampicillin ,Streptococcus pneumoniae ,Drug Resistance, Bacterial ,Gram-Negative Bacteria ,medicine ,Humans ,Pharmacology (medical) ,Respiratory Tract Infections ,Surveillance ,Bacterial Infections ,biology.organism_classification ,Anti-Bacterial Agents ,Ciprofloxacin ,Infectious Diseases ,Amikacin ,Susceptibility ,medicine.drug - Abstract
For the purpose of a nationwide surveillance of the antimicrobial susceptibility of bacterial respiratory pathogens in patients in Japan, the Japanese Society of Chemotherapy conducted their second year survey, during the period from January to August, 2007. A total of 1178 strains were collected from clinical specimens obtained from adult patients with well-diagnosed respiratory tract infections. Susceptibility testing was evaluable for 1108 strains (226 Staphylococcus aureus, 257 Streptococcus pneumoniae, 6 Streptococcus pyogenes, 206 Haemophilus influenzae, 120 Moraxella catarrhalis, 122 Klebsiella pneumoniae, and 171 Pseudomonas aeruginosa). A total of 44 antibacterial agents, including 26 beta-lactams (four penicillins, three penicillins in combination with beta-lactamase inhibitors, four oral cephems, eight parenteral cephems, one monobactam, five carbapenems, and one penem), three aminoglycosides, four macrolides (including ketolide), one lincosamide, one tetracycline, two glycopeptides, six fluoroquinolones, and one oxazolidinone were used for the study. Analysis was conducted at the central reference laboratory according to the method recommended by the Clinical and Laboratory Standards Institute (CLSI). The incidence of methicillinresistant Staphylococcus aureus (MRSA) was high, at 59.7%, and the incidences of penicillin-intermediateresistant and -resistant Streptococcus pneumoniae (PISP and PRSP) were 30.4% and 5.1%, respectively. Among Haemophilus influenzae strains, 19.9% of them were found to be beta-lactamase-non-producing ampicillin (ABPC)-intermediately-resistant (BLNAI), 29.1% to be beta-lactamasenon-producing ABPC-resistant (BLNAR), and 6.7% to be beta-lactamase-producing ABPC-resistant (BLPAR) strains. Extended-spectrum beta-lactamase-producing Klebsiella pneumoniae was not isolated. Two isolates (1.2%) of Pseudomonas aeruginosa were found to be metallo-beta-lactamase-producing strains, including one (0.6%) suspected multidrug-resistant strain showing resistance to imipenem, amikacin, and ciprofloxacin. These data will be a useful reference for future periodic surveillance studies and for investigations to control resistant infections as well. Continued surveillance is required to prevent the further spread of these antimicrobial resistances.
- Published
- 2009
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9. Principle in Imaging Contrast in Scanning Electron Microscopy for Binary Microstructures Composed of Organosilane Self-Assembled Monolayers
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Y. Wu, Osamu Takai, and H. Sugimura, K. Hayashi, and Nagahiro Saito
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Materials science ,Silicon ,Scanning electron microscope ,Ab initio ,chemistry.chemical_element ,Self-assembled monolayer ,Surfaces, Coatings and Films ,law.invention ,Crystallography ,chemistry ,law ,Electron affinity ,Monolayer ,Materials Chemistry ,Molecular orbital ,Physical and Theoretical Chemistry ,Electron microscope - Abstract
Field-emission electron microscopy (FE-SEM) was applied to observe coplanar microstructures composed of two different types of organosilane self-assembled monolayers (SAMs). These binary microstructures were prepared on silicon substrates covered with native oxide by a lithographic technique. Four types of organosilane precursors, they are n-octadecyltrimethoxysilane (ODS), heptadecafluoro-1,1,2,2-tetrahydro-decyl-1-trimethoxysilane (a type of fluoroalkylsilane, FAS), n-(6-aminohexyl)aminopropyltrimethoxysilane (AHAPS), and 4-(chloromethyl)phenyltrimethoxysilane (CMPhS), were used in this study. Micropatterns composed of the SAMs were clearly imaged by FE-SEM at low acceleration voltages, around 0.6 kV. The brightness order of the SAMs in FE-SEM was ODS > AHAPS > CMPhS > FAS. Through ab initio molecular orbital calculations, the origin of this FE-SEM contrast was ascribed to the electron affinity between the SAMs, which governed the FE-SEM image contrast. It has been successfully demonstrated that FE-SEM ...
- Published
- 2002
10. SGOL1 (shugoshin-like 1 (S. pombe))
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T Kahyo and H Sugimura
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Genetics ,Cancer Research ,Hematology ,Cell cycle ,Biology ,chemistry.chemical_compound ,Shugoshin-Like 1 ,Oncology ,chemistry ,Chromosome 3 ,Mitosis ,Gene ,DNA - Abstract
Review on SGOL1 (shugoshin-like 1 (S. pombe)), with data on DNA, on the protein encoded, and where the gene is implicated.
- Published
- 2013
11. Excitation states of titanium and nitrogen gas in an electron beam evaporation sustained arc
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K. Tsuzuki, Osamu Takai, S. Q. Xiao, and H. Sugimura
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Analytical chemistry ,chemistry.chemical_element ,Surfaces and Interfaces ,Condensed Matter Physics ,Electron beam physical vapor deposition ,Surfaces, Coatings and Films ,Electric arc ,symbols.namesake ,Plasma arc welding ,chemistry ,Excited state ,symbols ,Langmuir probe ,Plasma diagnostics ,Atomic physics ,Excitation ,Titanium - Abstract
The arc discharge studied in this work was sustained by titanium vapor evaporated by an electron beam in nitrogen atmosphere. Optical emission spectroscopy and Langmuir probe diagnostics were carried out to study the excitation of titanium and nitrogen. It was found that with increasing arc current, Ti and nitrogen gas exhibited an opposite tendency of excitation. When arc current increased from 20 to 60 A, Ti was highly excited to Ti+, while the excitation of nitrogen was lowered; this is completely different from that for a conventional cathodic arc, where both Ti and N2 are highly ionized. By Langmuir probe diagnostics, the electron energy distribution followed a two-Maxwellian distribution at an arc current of 60 A; the electron temperatures were 0.98 and 10.1 eV, and the corresponding electron densities were 2.7×1011 and 3.9×109 cm3, respectively. This result provided a theoretical explanation for the difference of excitation between titanium and nitrogen in this arc.
- Published
- 1999
12. Shaking Table Tests to investigate the seismic stability of preloaded and prestressed reinforced soil
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Masahiro Shinoda, T. Ushimura, H. Sugimura, T. Kikuchi, and Fumio Tatsuoka
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Seismic stability ,business.industry ,Earthquake shaking table ,Geotechnical engineering ,Structural engineering ,business ,Geology - Published
- 1999
13. [Untitled]
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K. Tsuzuki, S. Q. Xiao, H. Sugimura, and Osamu Takai
- Subjects
Materials science ,Mechanical Engineering ,Ion plating ,Analytical chemistry ,Mineralogy ,chemistry.chemical_element ,Nitride ,Electric arc ,Cerium ,Lattice constant ,chemistry ,Mechanics of Materials ,Electrical resistivity and conductivity ,Knoop hardness test ,General Materials Science ,Thin film - Abstract
Cerium nitride films were deposited by ion plating in an electron-beam sustained Ce arc discharge in a nitrogen atmosphere. The crystal structure was strongly affected by the arc discharge current and the substrate temperature. The lattice spacing of CeN film is 0.5020 nm with a density of 7.82 g cm−3. This film showed a paramagnetic property at 10 K in a magnetic field of 20 kOe. The Knoop hardness for CeN film is over 1600. The electrical resistivity was 4.6 × 10−4 Ωcm with p-type conductivity. The carrier concentration of the CeN film increased after exposure to the air, which suggested that the valence of Ce in CeN is probably 4+.
- Published
- 1998
14. Ethnic differences in GRHPR mutations in patients with primary hyperoxaluria type 2
- Author
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T, Takayama, N, Takaoka, M, Nagata, K, Johnin, Y, Okada, S, Tanaka, M, Kawamura, T, Inokuchi, M, Ohse, T, Kuhara, F, Tanioka, H, Yamada, H, Sugimura, and S, Ozono
- Subjects
Adult ,Male ,Infant ,Polymorphism, Single Nucleotide ,White People ,Alcohol Oxidoreductases ,Asian People ,Child, Preschool ,Hyperoxaluria, Primary ,Mutation ,Ethnicity ,Humans ,Female ,Child ,Sequence Deletion - Abstract
The objective of this study was to investigate ethnic differences in the glyoxylate reductase/hydroxypyruvate reductase (GRHPR) gene in patients with primary hyperoxaluria type 2 (PH2). GRHPR was genotyped in Japanese patients with PH2 and all GRHPR mutations described to date were reviewed in terms of geographic and ethnic association. We identified a novel mutation, a two-nucleotide deletion (c.248_249delTG) in exon 3 creating a premature 'stop' at codon 91. Also, we found that the c.864_865delTG mutation was associated with the rs35891798 single-nucleotide polymorphism. The allelic frequencies of the c.103delG, c.494GA, c.403_404+2 delAAGT, and c.864_865delTG mutations in PH2 patients were 37.8%, 15.6%, 10.0%, and 10.0%, respectively. All patients with the c.103delG mutation were Caucasian. Patients with the c.494GA mutation and 78% (7/9) of those with the c.403_404+2 delAAGT mutation were from the Indian subcontinent, whereas those with the c.864_865delTG mutation were Chinese or Japanese. Molecular analysis of GRHPR of four Japanese PH2 patients identified a novel mutation (c.248_249delTG in exon 3). Caucasians with PH2 should be screened for the c.103delG mutation; patients from the Indian subcontinent for c.494GA; and patients of East Asian origin (particularly) for c.864_865delTG. The prevalence of the latter mutation in PH2 patients from East Asia was 75.0%.
- Published
- 2013
15. Search for the θ+ pentaquark via the π-p→K-X reaction at 1.92GeV/c
- Author
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K, Shirotori, T N, Takahashi, S, Adachi, M, Agnello, S, Ajimura, K, Aoki, H C, Bhang, B, Bassalleck, E, Botta, S, Bufalino, N, Chiga, P, Evtoukhovitch, A, Feliciello, H, Fujioka, F, Hiruma, R, Honda, K, Hosomi, Y, Ichikawa, M, Ieiri, Y, Igarashi, K, Imai, N, Ishibashi, S, Ishimoto, K, Itahashi, R, Iwasaki, C W, Joo, M J, Kim, S J, Kim, R, Kiuchi, T, Koike, Y, Komatsu, V V, Kulikov, S, Marcello, S, Masumoto, K, Matsuoka, K, Miwa, M, Moritsu, T, Nagae, M, Naruki, M, Niiyama, H, Noumi, K, Ozawa, N, Saito, A, Sakaguchi, H, Sako, V, Samoilov, M, Sato, S, Sato, Y, Sato, S, Sawada, M, Sekimoto, H, Sugimura, S, Suzuki, H, Takahashi, T, Takahashi, H, Tamura, T, Tanaka, K, Tanida, A O, Tokiyasu, N, Tomida, Z, Tsamalaidze, M, Ukai, K, Yagi, T O, Yamamoto, S B, Yang, Y, Yonemoto, C J, Yoon, and K, Yoshida
- Subjects
COLLISIONS ,NUCLEI ,BARYON RESONANCE ,POSITIVE STRANGENESS ,THRESHOLD ,pentaquark - Abstract
The Θ(+) pentaquark baryon was searched for via the π(-)p→K(-)X reaction with a missing mass resolution of 1.4 MeV/c(2) (FWHM) at the Japan Proton Accelerator Research Complex (J-PARC). π(-) meson beams were incident on the liquid hydrogen target with a beam momentum of 1.92 GeV/c. No peak structure corresponding to the Θ(+) mass was observed. The upper limit of the production cross section averaged over the scattering angle of 2° to 15° in the laboratory frame is obtained to be 0.26 μb/sr in the mass region of 1.51-1.55 GeV/c(2). The upper limit of the Θ(+) decay width is obtained to be 0.72 and 3.1 MeV for J(Θ)(P)=1/2(+) and J(Θ)(P)=1/2(-), respectively, using the effective Lagrangian approach.
- Published
- 2012
16. EPHA7 (EPH receptor A7)
- Author
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T Bunai, H Sugimura, Masaya Suzuki, and H Mori
- Subjects
Genetics ,Cancer Research ,Erythropoietin-producing hepatocellular (Eph) receptor ,EPHA7 ,Hematology ,Biology ,biological factors ,Mini review ,chemistry.chemical_compound ,Oncology ,EPH receptor A3 ,chemistry ,biological phenomena, cell phenomena, and immunity ,Gene ,DNA - Abstract
Review on EPHA7 (EPH receptor A7), with data on DNA, on the protein encoded, and where the gene is implicated.
- Published
- 2011
17. Impact of supervisor support on work ability in an IT company
- Author
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H. Sugimura and Gilles Thériault
- Subjects
Adult ,Employment ,Male ,Longitudinal study ,Informatics ,Psychometrics ,Job control ,education ,Applied psychology ,Information science ,Job Satisfaction ,Young Adult ,Professional Competence ,health services administration ,Humans ,Staff Development ,Tokyo ,Occupational Health ,Supervisor ,ComputingMilieux_THECOMPUTINGPROFESSION ,Public Health, Environmental and Occupational Health ,Social Support ,Regression analysis ,Middle Aged ,Work (electrical) ,Scale (social sciences) ,ComputingMilieux_COMPUTERSANDSOCIETY ,Female ,Psychology ,Epidemiologic Methods ,human activities - Abstract
Background Work ability is the ability of a worker to perform his job. The authors hypothesized that supervisor support influences the work ability of workers working in an information technology company located in Tokyo. Aims To assess whether or not support from supervisors influences work ability. Methods Two surveys using the Brief Job Scale Questionnaire and the Work Ability Index (WAI) were conducted, one in October 2007 and the other in October 2008 on the same cohort. Two cross-sectional analyses and a 1-year longitudinal analysis were conducted using multiple regression analysis. In addition, the relationships between supervisor support and each dimension of WAI were analysed separately. Results The number of participants was 1157 males. Significant relationships were observed between supervisor support and WAI scores in both survey periods after adjusting for age, job demand, job control, work group size, job rank and job type. The 2007 Supervisor support was a significant predictor of 2008 WAI that raised the possibility that supervisor support does influence WAI scores. From the analysis of each dimension of WAI, a strong relationship between supervisor support and WAI was observed for the sections of the WAI that assessed work capacity but not for the sections that assessed the personal health status of respondents. Conclusions Supervisor support is an important predictor of work ability. Supervisor support is associated with the questions of the WAI that assess not only work demands but also person's resources of the work ability model.
- Published
- 2010
18. A novel STK11 germline mutation in two siblings with Peutz-Jeghers syndrome complicated by primary gastric cancer
- Author
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K, Shinmura, M, Goto, H, Tao, S, Shimizu, Y, Otsuki, H, Kobayashi, S, Ushida, K, Suzuki, T, Tsuneyoshi, and H, Sugimura
- Subjects
Adult ,Family Health ,Heterozygote ,Siblings ,DNA Mutational Analysis ,Peutz-Jeghers Syndrome ,Protein Serine-Threonine Kinases ,Cadherins ,Genes, p53 ,Pedigree ,AMP-Activated Protein Kinase Kinases ,Stomach Neoplasms ,Humans ,Female ,Genetic Predisposition to Disease ,Frameshift Mutation ,Germ-Line Mutation - Abstract
Patients with Peutz-Jeghers syndrome (PJS) are known to be at risk of gastric cancer (GC), and the STK11 gene is a susceptibility gene for PJS. However, as no cases of PJS with GC in which a STK11 germline mutation has been identified have ever been reported and other susceptibility genes have also been suggested to be involved in PJS, the relation between STK11 germline mutations and GC in PJS is still unknown. In this study, we used sequencing analysis to investigate the STK11, CDH1, and TP53 loci for a germline mutation in two siblings with PJS with primary GC. A novel type of the STK11 germline mutation, c.890delG, encoding a truncated protein (p.Arg297fsX38) was identified, but no germline mutations of the CDH1 and TP53 genes were detected. No inactivation of the wild-type allele by somatic mutation or chromosomal deletion or hypermethylation at the 5'-CpG site of STK11 was detected in the GC. This is the first report of a STK11 germline mutation in a PJS patient with GC and should contribute to establishing correlations between the STK11 germline mutations and GC in PJS patients.
- Published
- 2004
19. Aligned bioactive mesoporous silica coatings for implants
- Author
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J M, Gomez-Vega, H, Sugimura, O, Takai, and A, Hozumi
- Abstract
Ongoing research is reported aimed at preparing mesoporous silica coatings on various substrates for medical applications by a biomimetic approach (self-assembling of organic/inorganic sol-gel systems into ordered structures). Tetraethylorthosilicate (TEOS) was selected as the silica precursor, and amphiphilic triblock copolymers poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide), and the cationic surfactant cetyltrimethyl ammonium chloride (CTAC), as structure-directing agents. The mesochannels diameter could be adjusted by changing the directing agent, and a preferred alignment of the mesostructure was observed independently of the used substrate (glass, silicon, Ti or Ti6Al4V). Three different treatments (thermocalcination, photocalcination, and solvent extraction) have been also studied to remove the organic templates, of which photocalcination showed to be the most versatile. When soaked in a simulated body fluid, mesoporous silica coatings induced apatite formation after seven days.
- Published
- 2004
20. [Mutation analysis of tumor suppressor gene PTEN in bone and soft tissue tumors]
- Author
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Y, Zhu, D, Wang, and H, Sugimura
- Subjects
Asian People ,Tumor Suppressor Proteins ,DNA Mutational Analysis ,PTEN Phosphohydrolase ,Humans ,Bone Neoplasms ,Genes, Tumor Suppressor ,Soft Tissue Neoplasms ,Genetic Testing ,Polymerase Chain Reaction ,Polymorphism, Single Nucleotide ,Phosphoric Monoester Hydrolases - Abstract
To study tumor suppressor gene PTEN's role in the tumorigenesis of bone and soft tissue tumors.Exon 4 approximately 9 of PTEN were amplified by polymerase chain reaction (PCR) for homozygous deletions in 110 tumor samples of patients with bone and soft tissue tumors, 3 tumor cell lines. PTEN mutations were detected by a combination of single strand conformation polymorphism (SSCP) analysis and DNA sequencing. Normal tissue beside tumors was used as control.There were always products of amplified exon 4 approximately 9 of PTEN, so no homozygous deletions existed. No homozygous deletions presented as variant band was not found by SSCP analysis. Two kinds of band type appeared in exon 8. A g/t polymorphism was found 32 bp from the splice donor site of intron 8 in six tumors.At the level of DNA, PTEN mutations do not play major roles in the tumorigenesis of bone and soft tissue tumors. The polymorphism of PTEN gene exists in Chinese people.
- Published
- 2002
21. Population-based mapping of pulmonary adenoma susceptibility 1 locus
- Author
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T A, Dragani, S, Hirohashi, T, Juji, K, Kawajiri, M, Kihara, M, Ono-Kihara, G, Manenti, T, Nomoto, H, Sugimura, K, Genka, J, Yokota, T, Takahashi, T, Mitsudomi, and M, Nagao
- Subjects
Genetic Markers ,Male ,Lung Neoplasms ,Polymorphism, Genetic ,Chromosome Mapping ,Pilot Projects ,Adenocarcinoma ,Middle Aged ,Prognosis ,Survival Analysis ,Linkage Disequilibrium ,Proto-Oncogene Proteins p21(ras) ,Case-Control Studies ,Proto-Oncogene Proteins ,Carcinoma, Squamous Cell ,ras Proteins ,Humans ,Female ,Genetic Predisposition to Disease ,Deoxyribonucleases, Type II Site-Specific ,Alleles ,Aged - Abstract
Pulmonary adenoma susceptibility 1 (Pas1), the major locus affecting inherited predisposition to lung tumor development in mice, maps near the Kras2 gene. We previously reported a significant association between a KRAS2/RsaI polymorphism and the risk and prognosis of lung adenocarcinoma (ADCA) in the Italian population. In the present case-control study, we examined 269 lung ADCA patients, 121 squamous cell lung carcinoma patients, and 632 healthy individuals (general population controls) in the Japanese population with genetic markers spanning approximately 1200 kb in the KRAS2 region. Allele-specific oligonucleotide hybridization revealed the same KRAS2/RsaI polymorphism associated with risk and prognosis as in Italian lung ADCA patients; the polymorphism was significantly associated with clinical stage (P0.001) and survival rate (log rank = 0.0014), confirming the mapping of PAS1 and pointing to the role of this locus in human lung cancer.
- Published
- 2000
22. Different vulnerability among chromosomes to numerical instability in gastric carcinogenesis: stage-dependent analysis by FISH with the use of microwave irradiation
- Author
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Y, Kitayama, H, Igarashi, and H, Sugimura
- Subjects
Adult ,Aged, 80 and over ,Chromosome Aberrations ,Sex Chromosomes ,Middle Aged ,Prognosis ,Sensitivity and Specificity ,Stomach Neoplasms ,Humans ,Female ,Chromosome Deletion ,Microwaves ,In Situ Hybridization, Fluorescence ,Aged ,Neoplasm Staging - Abstract
Numerical chromosomal abnormalities are a well known characteristic of human cancer, but no "chromosome-wide investigation" encompassing almost all of the chromosomes has ever been reported. Furthermore, although the multistep process of carcinogenesis is widely accepted in human cancer, the stepwise numerical aberration of chromosomes has never been addressed. Touch preparations of 24 (male 20, female 4) surgically resected gastric cancer tissue samples in various stages in terms of depth of invasion were analyzed by fluorescence in situ hybridization using centromere-specific probes including 17 chromosomes, 1-4, 6-8, 10-12, 15-18, 20, X, and Y. Microwave irradiation was performed to increase the sensitivity and specificity of the signal. The depth of the tumor invasion in the gastric wall and histological subtypes were recorded by viewing the histology of the adjacent portion. Numerical chromosomal abnormalities of chromosomes 1 and 2 were found most frequently and from the early stage of gastric cancer. The abnormalities observed were limited to chromosomes 1, 2, 4, and 20 in tumors invading to the middle layer of the submucosa of the gastric wall, but these became more extensive, involving almost all of the chromosomes investigated when the tumor had invaded beyond the proper muscle of the gastric wall. Centromeric numbers of chromosomes 3 and 18 were exceptionally stable even after the tumor progressed to advanced stage. These profiles of the sequential process of numerical chromosomal abnormality were similar in both mucocellular and tubular-type gastric cancer, but the prevalence was significantly lower in the mucocellular type (39.0% versus 68.0%). On the basis of fluorescence in situ hybridization analysis of 17 different chromosome centromeres in gastric cancer in various stages, we conclude that the earliest events in gastric carcinogenesis in terms of chromosomal abnormality occur in chromosomes 1 and 2 and that chromosomal numerical aberrations expand in a stepwise manner with cancer progression.
- Published
- 2000
23. Cytochrome P450 2E1 polymorphism in gastric cancer in Brazil: case-control studies of Japanese Brazilians and non-Japanese Brazilians
- Author
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I N, Nishimoto, T, Hanaoka, H, Sugimura, K, Nagura, M, Ihara, X J, Li, T, Arai, G S, Hamada, L P, Kowalski, and S, Tsugane
- Subjects
Male ,Polymorphism, Genetic ,Genotype ,Cytochrome P-450 CYP2E1 ,Middle Aged ,Risk Assessment ,Diet ,Japan ,Stomach Neoplasms ,Case-Control Studies ,Humans ,Female ,Genetic Predisposition to Disease ,Brazil ,Aged - Abstract
Cytochrome P450 2E1 (Cyp2E1) is involved in the metabolic oxidation of carcinogenic nitroso compounds, including N-nitrosoamines. There is an RsaI polymorphism in the transcriptional regulatory region of this gene, and in vitro evidence suggests that the variant type of this polymorphic site has higher transcriptional activity but less chlorzoxazone-metabolizing activity. Interindividual differences in the metabolic capacity of Cyp2E1 are assumed to be associated with cancer susceptibility, but the results of the previous studies on the relation between Cyp2E1 RsaI polymorphism and cancer susceptibility have been inconsistent. Two case-control studies of gastric cancer in Japanese Brazilians (96 cases, 192 controls) and Brazilians not of Japanese ancestry (non-Japanese Brazilians; 236 cases, 236 controls) in São Paulo were designed to clarify the role of the Cyp2E1 RsaI genotype in susceptibility to gastric cancer after considering multifactorial environmental influences. The subjects with variant RsaI genotypes amounted to 47% (28 of 59) and 48% (64 of 133), respectively, of the Japanese cases and controls, and 6% (11 of 187) and 10% (19 of 192), respectively, of the non-Japanese cases and controls. As expected, a difference in the distributions of the two groups was observed. The odds ratio of the RsaI variant genotype of Cyp2E1 was 0.46 (95% confidence interval, 0.21-1.04) in the non-Japanese Brazilian population and 0.98 (95% confidence interval, 0.50-1.90) in the Japanese Brazilian population after adjusting for sex, age, tobacco use, and meat consumption. Additional adjustment for potential confounding factors did not change the odds ratio substantially. No significant interactions were observed between the polymorphism and environmental factors. In regard to the histological type of gastric cancer, the variant genotype was significantly more prevalent than the common genotype in Japanese subjects with diffuse type gastric cancer. Our study suggests that the Cyp2E1 RsaI polymorphism is associated with a reduced risk of gastric cancer, although how the assumed increase in Cyp2E1 expression produced by this polymorphism is related to a reduced risk of cancer remains unclear. The observations in this study are consistent with the recent observations of esophageal cancer in endemic areas of China.
- Published
- 2000
24. Presence of telomeric G-strand tails in the telomerase catalytic subunit TERT knockout mice
- Author
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X, Yuan, S, Ishibashi, S, Hatakeyama, M, Saito, J, Nakayama, R, Nikaido, T, Haruyama, Y, Watanabe, H, Iwata, M, Iida, H, Sugimura, N, Yamada, and F, Ishikawa
- Subjects
Cell Extracts ,Male ,Mice, Knockout ,Molecular Sequence Data ,DNA ,Electrophoresis, Gel, Pulsed-Field ,DNA-Binding Proteins ,Mice, Inbred C57BL ,Mice ,Catalytic Domain ,Testis ,Animals ,Humans ,RNA ,Amino Acid Sequence ,Telomerase ,HeLa Cells - Abstract
Telomerase consists of two essential subunits, the template RNA (TR; telomerase RNA) and the catalytic subunit TERT (telomerase reverse transcriptase). Knockout mice with a mTR (mouse TR) deletion have been described and well characterized. However, mice with a mTERT (mouse TERT) deletion have not been reported.mTERT-knockout mice have been constructed. The first generation mTERT -/- mice were fertile, and did not show any noticeable macroscopic or microscopic phenotypic change. All tissue cells derived from mTERT -/- mice that were examined lacked telomerase activity, indicating that mTERT is the only gene encoding the telomerase catalytic subunit. Pulse field gel electrophoresis (PFGE) and nondenaturing in-gel hybridization analyses showed that mouse telomeric DNA has G-strand 5'-overhangs, as demonstrated for human and yeast cells. This telomeric single-stranded G-tail was also observed in MEF (mouse embryonic fibroblast) and liver cells derived from mTERT -/- mice.mTERT-knockout mice show phenotypes that are apparently normal at least during the early generations. This observation is similar to that obtained with the mTR-knockout mice. The presence of the telomeric G-strand tails in mTERT -/- mice suggests that these telomeric 5'-overhangs are produced by telomerase-independent mechanisms, as has been proposed for yeast and human.
- Published
- 1999
25. Association of Ile462Val (Exon 7) polymorphism of cytochrome P450 IA1 with lung cancer in the Asian population: further evidence from a case-control study in Okinawa
- Author
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H, Sugimura, K, Wakai, K, Genka, K, Nagura, H, Igarashi, K, Nagayama, A, Ohkawa, S, Baba, B J, Morris, S, Tsugane, Y, Ohno, C, Gao, Z, Li, T, Takezaki, K, Tajima, and T, Iwamasa
- Subjects
Adult ,Aged, 80 and over ,Male ,China ,Lung Neoplasms ,Polymorphism, Genetic ,Australia ,Valine ,Exons ,Middle Aged ,White People ,Amino Acid Substitution ,Asian People ,Japan ,Case-Control Studies ,Cytochrome P-450 CYP1A1 ,Humans ,Isoleucine ,Aged - Abstract
Okinawa, a group of islands that lie between the East China Sea and the Pacific Ocean, 2000 km south of the Japanese main islands, has a different profile of diseases, ethnicities, and cultures than does the rest of Japan. We examined an Ile462Val polymorphism (CYP1A1*2 allele) of cytochrome P450 IA1 in a hospital-based case-control study of lung cancer patients (247 cases and 185 controls) in Okinawa to ascertain the association of this variant with lung cancer. In addition, the distribution of this genotype was studied in populations from different areas of Japan, including Tokyo (n = 69) and Iwate (northern part of Japan; n = 81), as well as in a Chinese group from the Jiangsu province (n = 39) and in an Australian Caucasian group (n = 146). Genotype frequency in controls was not significantly different from area to area in Japan. In Okinawa, however, the genotype encoding Val/Val was associated with a significantly higher risk of lung cancer (odds ratio = 3.32, P = 0.013), especially of squamous cell carcinoma and small cell carcinoma (odds ratio = 4.85 and 9.35, respectively). The Val-encoding allele was less frequent in the Chinese population and was rare in Australian Caucasians. Thus, this study gives support to the value of the cytochrome P450 IA1 Ile462Val polymorphism as a practical high-risk marker of lung cancer in populations, especially those in southeast Asia, in which this variant is more common.
- Published
- 1998
26. Stage-dependent evaluation of microsatellite instability in gastric carcinoma with familial clustering
- Author
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K, Shinmura, W, Yin, J, Isogaki, K, Saitoh, K, Kanazawa, K, Koda, J, Yokota, I, Kino, T, Arai, and H, Sugimura
- Subjects
Adult ,Male ,Heterozygote ,DNA, Neoplasm ,Middle Aged ,Genes, p53 ,Asian People ,Japan ,Stomach Neoplasms ,Case-Control Studies ,Cluster Analysis ,Humans ,Female ,Neoplasm Invasiveness ,Germ-Line Mutation ,Aged ,Microsatellite Repeats ,Neoplasm Staging - Abstract
Familial clustering of gastric cancer is probably caused by multifactorial processes, both environmental and genetic. In this report, the incidence of microsatellite instability (MSI) in 31 cases of gastric cancer in Japanese (33 lesions) with familial clustering (two or more gastric cancers within second-degree relatives) was compared to MSI in Japanese cases without a family of any cancer in age ( +/- 10 years)-, stage-, and histological subtype-matched case-control study. Although the difference noted was not significant, we noted a strong trend for MSI at any of up to seven loci of CA repeats to occur more frequently in the patients with a family history of gastric than in the control patients in early cancer (intramucosal and submucosal), whereas the prevalence of MSI was similar in both groups in more advanced cases, in which the tumor invaded beyond the proper muscle layer of the gastric wall. Because the contribution of a family history of gastric cancer to MSI apparently differs in early and advanced gastric cancer, interpretation of MSI in familial gastric cancer cases published previously require reevaluation in terms of stage and proper controls. An acquisition of CA repeat alterations in the early stage rather than in the late stage of gastric carcinogenesis may have in common etiological factors, at least in some cases, with the familial clustering of gastric cancer.
- Published
- 1997
27. Deficient transforming growth factor-beta1 activation and excessive insulin-like growth factor II (IGFII) expression in IGFII receptor-mutant tumors
- Author
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S, Wang, R F, Souza, D, Kong, J, Yin, K N, Smolinski, T T, Zou, T, Frank, J, Young, K C, Flanders, H, Sugimura, J M, Abraham, and S J, Meltzer
- Subjects
Membrane Glycoproteins ,Insulin-Like Growth Factor II ,Stomach Neoplasms ,Transforming Growth Factor beta ,Mutation ,Humans ,Colorectal Neoplasms ,Immunohistochemistry ,Receptors, Fibroblast Growth Factor ,Receptor, IGF Type 2 ,Microsatellite Repeats - Abstract
The insulin-like growth factor II receptor (IGFIIR) gene has been identified as a coding region target of microsatellite instability in human gastrointestinal (GI) tumors. IGFIIR normally has two growth-suppressive functions: it binds and stimulates the plasmin-mediated cleavage and activation of the latent transforming growth factor-beta1 (LTGF-beta1) complex, and it mediates the internalization and degradation of IGFII ligand, a mitogen. We used an immunohistochemical approach to determine whether IGFIIR mutation affected expression of these proteins in GI tumors. Four highly specific antibodies were used: LC(1-30), which recognizes the active form of TGF-beta1; anti-LTGF-beta1, which detects the LTGF-beta1 precursor protein; anti-IGFIIR; and anti-IGFII ligand. Twenty GI tumors either with (6 of 20) or without (14 of 20) known IGFIIR mutation were examined, along with matching normal tissues. Results were statistically significant in the following categories: (a) decreased active TGF-beta1 protein expression in IGFIIR-mutant tumor tissues versus matching normal tissues or IGFIIR-wild-type tumor tissues; (b) increased LTGF-beta1 protein expression in IGFIIR-mutant tumor tissues versus matching normal tissues or IGFIIR-wild-type tumor tissues; and (c) increased IGFII ligand protein expression in IGFIIR-mutant tumor tissues versus matching normal tissues or IGFIIR-wild-type tumor tissues. These data suggest that in genetically unstable GI tumors, mutation of a microsatellite within the coding region of IGFIIR functionally inactivates this gene, causing both diminished growth suppression (via decreased activation of TGF-beta1) and augmented growth stimulation (via decreased degradation of the IGFII ligand).
- Published
- 1997
28. The heme-binding region polymorphism of cytochrome P450IA1 (CypIA1), rather than the RsaI polymorphism of IIE1 (CypIIE1), is associated with lung cancer in Rio de Janeiro
- Author
-
G S, Hamada, H, Sugimura, I, Suzuki, K, Nagura, E, Kiyokawa, T, Iwase, M, Tanaka, T, Takahashi, S, Watanabe, and I, Kino
- Subjects
Male ,Lung Neoplasms ,Polymorphism, Genetic ,Genotype ,Genetic Linkage ,Homozygote ,Smoking ,Cytochrome P-450 CYP2E1 ,Oxidoreductases, N-Demethylating ,Valine ,Exons ,Heme ,Middle Aged ,Deoxyribonuclease HpaII ,Cytochrome P-450 Enzyme System ,Case-Control Studies ,Carcinoma, Squamous Cell ,Ethnicity ,Humans ,Female ,Genetic Predisposition to Disease ,Isoleucine ,Deoxyribonucleases, Type II Site-Specific ,Brazil ,Protein Binding - Abstract
Ile-Val polymorphism in exon 7 of cytochrome P450IA1 (CypIA1) and RsaI polymorphism of cytochrome P450IIE1 (CypIIE1) were examined in a case-control study of lung cancer in Rio de Janeiro, Brazil. The Val-containing genotype in exon 7 of CypIA1 was found to be associated with lung cancer in this population (odds ratio, 2.26; 95% confidence interval, 1.14-4.47 for 99 cases versus 108 controls of 123 matched pairs), whereas RsaI polymorphism in CypIIE1 was not associated with lung cancer susceptibility. In squamous cell carcinoma, the degree of association of Val-containing genotype was greater in those with fewer pack-years of smoking. The RsaI polymorphism of CypIIE1 has a different distribution from the Japanese pattern and is not associated with lung cancer. When we analyzed the association of Ile-Val polymorphism to MspI polymorphism of CypIA1, the Val/Val homozygote was found only in the subpopulation with the MspI site-present homozygote. The apparent lack of association of CypIA1 MspI polymorphism with lung cancer in this area reported in our previous study and the results of the present study indicate that the "true" responsible site for lung cancer susceptibility should be the Ile-Val polymorphism in the catalytic site of CypIA1.
- Published
- 1995
29. Analysis of cytochrome P450 2E1 genetic polymorphisms in relation to human lung cancer
- Author
-
S, Kato, P G, Shields, N E, Caporaso, H, Sugimura, G E, Trivers, M A, Tucker, B F, Trump, A, Weston, and C C, Harris
- Subjects
Male ,Lung Neoplasms ,Genotype ,Transcription, Genetic ,Black People ,White People ,Asian People ,Cytochrome P-450 Enzyme System ,Gene Frequency ,Japan ,Risk Factors ,Humans ,Alleles ,Aged ,Aged, 80 and over ,Polymorphism, Genetic ,Gene Amplification ,Cytochrome P-450 CYP2E1 ,Oxidoreductases, N-Demethylating ,Middle Aged ,Introns ,United States ,Haplotypes ,Case-Control Studies ,Female ,Polymorphism, Restriction Fragment Length - Abstract
Human cancer risk assessment using molecular genetic techniques is a rapidly emerging field. Many studies suggest that both inherited and acquired genetic predispositions play an important role in carcinogenesis. Cytochrome P450 (CYP) 2E1 is involved in the metabolic activation of N-nitrosamines and other low molecular weight compounds. A recently described genetic polymorphism of CYP2E1 [DraI restriction fragment length polymorphism (RFLP)] has been associated with an increased risk of lung cancer in Japanese. We have assessed the allelic frequency of three RFLPs (PstI, RsaI, and DraI) in African-Americans (n = 109), Caucasian Americans (n = 153), and octogenarian Japanese (n = 42), and also in a United States case-control study of lung cancer (histologically confirmed lung cancer, n = 58; controls, n = 56; total, n = 114). The relationship of the CYP2E1 DraI polymorphism to other CYP2E1 polymorphisms (PstI and RsaI RFLP) was examined. The allelic frequency of the DraI C minor allele for all subjects was 0.09 in Caucasians, 0.09 in African-Americans, and 0.31 in Japanese. In the case-control study of lung cancer, no association of the CYP2E1 DraI genotype with lung cancer was found (odds ratio, 1.57; 95% confidence interval, 0.59-4.18). Comparison after discordant CYP2E1 genotypes suggests the presence of different haplotypes in Americans and Japanese. These results indicate that the CYP2E1 DraI RFLP is probably not a cancer risk factor in United States Caucasian or African-Americans, although statistical power is limited given the low frequency of the CYP2E1 DraI C minor alleles.
- Published
- 1994
30. Overexpression of ERK, an EPH family receptor protein tyrosine kinase, in various human tumors
- Author
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E, Kiyokawa, S, Takai, M, Tanaka, T, Iwase, M, Suzuki, Y Y, Xiang, Y, Naito, K, Yamada, H, Sugimura, and I, Kino
- Subjects
Gene Expression Regulation, Neoplastic ,DNA, Complementary ,Base Sequence ,Receptor, EphB2 ,Neoplasms ,Molecular Sequence Data ,Humans ,Receptor Protein-Tyrosine Kinases ,Amino Acid Sequence ,RNA, Messenger ,In Situ Hybridization - Abstract
The ERK gene has been isolated as a genomic DNA encoding a part of the receptor protein-tyrosine kinase which belongs to the EPH subfamily. We previously identified a partial complementary DNA (cDNA) encompassing the catalytic domain of ERK from the expression library of human gastric cancer with an antiphosphotyrosine antibody. Using this cDNA as a probe, the cDNAs encoding mature ERK protein were isolated. The putative mature ERK protein, a total of 967 deduced amino acid residues, showed high homology with chicken Cek5 (92.5%) and mouse Nuk (99.1%). Chromosomal in situ hybridization revealed that human ERK cDNA is localized to chromosome 1p34-35. In Northern blot analysis of normal human tissues, the ERK gene was ubiquitously expressed mainly in cells of epithelial origin but not in the brain. Studies on RNAs from 76 human tumor tissues and cell lines showed that ERK is expressed at higher levels in various tumors of epithelial origin than in corresponding normal tissues, most frequently in gastric cancers (12 of 16, 75.0%). Overexpression of ERK was also detected in one osteosarcoma cell line. These findings suggest that ERK plays some significant role in carcinogenesis in the stomach and other tissues.
- Published
- 1994
31. Isolation of complementary DNA encoding K-cadherin, a novel rat cadherin preferentially expressed in fetal kidney and kidney carcinoma
- Author
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Y Y, Xiang, M, Tanaka, M, Suzuki, H, Igarashi, E, Kiyokawa, Y, Naito, Y, Ohtawara, Q, Shen, H, Sugimura, and I, Kino
- Subjects
DNA, Complementary ,Base Sequence ,Molecular Sequence Data ,Cadherins ,Kidney ,Kidney Neoplasms ,Rats ,Rats, Inbred ACI ,Fetus ,Gastric Mucosa ,Animals ,Amino Acid Sequence ,RNA, Messenger ,Rats, Wistar ,Carcinoma, Renal Cell ,In Situ Hybridization - Abstract
Complementary DNA for a novel member of the cadherin family, designated K-cadherin, was isolated from a rat renal cell carcinoma complementary DNA library by screening it with a short complementary DNA probe which was initially obtained from the RNA of day 16 fetal Wistar rat stomach mucosa by the polymerase chain reaction. The deduced primary structure of K-cadherin is 789 amino acid residues, which contain five internal repeats in its extracellular domain, a single putative transmembrane domain, and a cytoplasmic tail characteristic of those of classic type cadherins. K-cadherin exhibits low homology with mature proteins of mouse N- (38%), E- (35%), and P-cadherin (32%), and high homology with a partially identified human cadherin-6 protein (95%) at the amino acid level. Northern blot analysis revealed a high level of expression of K-cadherin mRNA in fetal rat kidney and brain, and rat kidney carcinoma with two major transcripts, 4.1 and 8.0 kilobases in size, whereas there was very weak or no expression in any organ of adult rats. The level of K-cadherin expression was also elevated in some human kidney cancer tissues. In the developing kidney, in situ hybridization showed localization of K-cadherin mRNA in the nephroblastic epithelial cells of comma bodies coinciding with those in the process of polarization during glomeruloneogenesis. These results demonstrate that K-cadherin must have important functions in both the process of kidney development and tumorigenesis of some types of kidney cancer.
- Published
- 1994
32. Cytochrome P-450 lA1 genotype in lung cancer patients and controls in Rio de Janeiro, Brazil
- Author
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H, Sugimura, I, Suzuki, G S, Hamada, T, Iwase, T, Takahashi, K, Nagura, H, Iwata, S, Watanabe, I, Kino, and S, Tsugane
- Subjects
Adult ,Cross-Cultural Comparison ,Male ,Lung Neoplasms ,Genotype ,Black People ,Middle Aged ,Cytochrome P-450 Enzyme System ,Risk Factors ,Carcinoma, Non-Small-Cell Lung ,Case-Control Studies ,Humans ,Female ,Carcinoma, Small Cell ,Brazil ,Polymorphism, Restriction Fragment Length ,Aged - Abstract
Mspl restriction fragment length polymorphism in cytochrome P-450 IA1 (CypIA1) gene, which has been associated with lung cancer susceptibility in Japanese, was studied in persons from Rio de Janeiro, in the framework of a hospital-based, age, race (black or nonblack), and gender-matched case-control study (n = 222; 110 cases and 112 controls). Contrary to the hypothesis, there was no difference in the frequency of the C genotype (Mspl site-present homozygous), even after racial breakdown. There were no significant differences between cases and controls when categorized according to tobacco consumption. The lifetime quantity of tobacco smoked was not different among lung cancer patients with three different genotypes (A, Mspl site-absent, homozygous; B, heterozygote; and C). The background frequency of the Mspl polymorphism C genotype is a little less than 10%, similar to that of the Japanese healthy population. The CyplA1 Mspl polymorphism itself does not seem to be related to susceptibility to bronchial carcinogenesis in this area.
- Published
- 1994
33. Lung cancer, race, and a CYP1A1 genetic polymorphism
- Author
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P G, Shields, N E, Caporaso, R T, Falk, H, Sugimura, G E, Trivers, B F, Trump, R N, Hoover, A, Weston, and C C, Harris
- Subjects
Heterozygote ,Lung Neoplasms ,Polymorphism, Genetic ,Genotype ,Homozygote ,Smoking ,Black People ,DNA, Neoplasm ,Polymerase Chain Reaction ,Asthma ,White People ,Blotting, Southern ,Cytochrome P-450 Enzyme System ,Gene Frequency ,Risk Factors ,Case-Control Studies ,Neoplasms ,Humans ,Lung Diseases, Obstructive - Abstract
The assessment of human cancer risk using molecular epidemiological techniques involves determining the relative contributions of inherited and acquired genetic predispositions, in the context of environmental exposures. Recently described genetic polymorphisms for CYP1A1, a gene involved in the metabolic activation of polycyclic aromatic hydrocarbons, have been associated with lung cancer risk in a Japanese population. We report herein findings from a United States case-control study of lung cancer (56 cases; 48 controls). The polymerase chain reaction followed by an Msp1 restriction enzyme digestion was used to analyze constitutive DNA but no association between the restriction fragment length polymorphism and lung cancer risk was found (odds ratio, 0.7; 95% confidence interval, = 0.3-1.6). Analysis of genotype by cumulative smoking status did not reveal an elevated risk among lesser or greater smokers. The presence of the CYP1A1 Msp1 site-present allele, which was previously found to be associated with Japanese lung cancer risk, was statistically increased in African compared to Caucasian Americans (odds ratio, 2.9; 95% confidence interval, 1.2-2.7). When stratified by race, however, no association between case status and the polymorphism was observed, but the small number of study subjects within each racial group limited the statistical power. Larger studies are required to evaluate the risk of the CYP1A1 Msp1 polymorphism in African Americans.
- Published
- 1993
34. Cytochrome P450IIE1 genetic polymorphisms, racial variation, and lung cancer risk
- Author
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S, Kato, P G, Shields, N E, Caporaso, R N, Hoover, B F, Trump, H, Sugimura, A, Weston, and C C, Harris
- Subjects
Lung Neoplasms ,Polymorphism, Genetic ,Genotype ,Racial Groups ,Cytochrome P-450 CYP2E1 ,Oxidoreductases, N-Demethylating ,Polymerase Chain Reaction ,Cytochrome P-450 Enzyme System ,Japan ,Case-Control Studies ,Baltimore ,Odds Ratio ,Humans ,Alleles - Abstract
Cytochrome P450IIE1 is responsible for the activation of carcinogenic N-nitrosamines, benzene, urethane, and other low-molecular-weight compounds. Restriction fragment length polymorphisms (PstI and RsaI restriction enzymes) have been identified in the cytochrome P450IIE1 transcription regulatory region that may affect expression. This study describes the PstI and RsaI polymorphisms in different racial populations and in a case-control study of lung cancer. The allelic frequencies were markedly different in Japanese, African-Americans, and Caucasians: the PstI rare allele was present at a frequency of 2% in Caucasians, 5% in African-Americans, and 24% in Japanese (P0.05). For the RsaI rare allele, frequencies were 2% in Caucasians, 2% in African-Americans, and 27% in Japanese (P0.05). The assay was also applied to 128 individuals enrolled in a case-control study of lung cancer. Although limited in statistical power, the data indicate no evidence for an association in the aggregate of cytochrome P450IIE1 PstI [for which the odds ratio was 0.7 (95% confidence interval (C.I.) = 0.2-2.8)] or RsaI [for which the odds ratio was 0.9 (95% C.I. = 0.2-5.4)] restriction fragment length polymorphisms with lung cancer in this U.S. population. When analyzed by race, the lung cancer odds ratio for the PstI mutant allele in African-Americans was 0.19 (95% C.I. = 0.03-1.38), and in Caucasians it was 4.13 (95% C.I. = 0.34-48.8). For the RsaI mutant allele, the odds ratios were 0.20 (95% C.I. = 0.02-2.43) and 4.28 (95% C.I. = 0.35-50.6), respectively. The ethnic differences of these restriction fragment length polymorphisms might be related to genetic susceptibilities for lung cancer among Caucasians and for gastric or esophageal cancer among Japanese.
- Published
- 1992
35. Association of rare alleles of the Harvey ras protooncogene locus with lung cancer
- Author
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H, Sugimura, N E, Caporaso, R V, Modali, R N, Hoover, J H, Resau, B F, Trump, J A, Longergan, T G, Krontiris, D L, Mann, and A, Weston
- Subjects
Genes, ras ,Lung Neoplasms ,Gene Frequency ,Case-Control Studies ,Humans ,DNA ,DNA, Neoplasm ,Lung Diseases, Obstructive ,Lymphocytes ,Adenocarcinoma ,Alleles ,Cells, Cultured - Abstract
The hypothesis that rare variable nucleotide tandem repeat alleles of the Ha-ras-1 polymorphism are an inherited predisposing factor in human lung carcinogenesis has been evaluated in an age, race, and smoking matched case-control study. Twenty-three different alleles were identified by their restriction fragment length in DNA isolated from peripheral blood lymphocytes and were categorized into three groups: common; intermediate; and rare. The frequencies of rare alleles in blacks with either squamous cell carcinoma, large cell carcinoma, or small cell carcinoma were found to be significantly higher than those among groups of control subjects that were comprised of chronic obstructive pulmonary disease patients and patients with cancer at sites other than the lung. A similar trend which did not reach statistical significance was observed in whites. These data are consistent with the hypothesis that inheritance of Ha-ras-1 rare restriction fragment length alleles represents a genetic risk factor for some human lung cancers. The biological basis of this observation remains to be clarified, and it is possible that ethnic variations in rare allele frequencies are responsible for the differences noted. However, the data suggest that further evaluation of the Ha-ras-1 polymorphism as a marker of individual lung cancer susceptibility is warranted.
- Published
- 1990
36. P1061 Investigation of usability for Event ECG in workplace. (2nd report)(Poster Presentation,Occupational Health in the Age of Decentralization Reform in Japan,The 79th Annual Meeting of Japan Society for Occupational Health)
- Author
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H. Sugimura
- Subjects
Medical education ,Event (computing) ,business.industry ,media_common.quotation_subject ,Public Health, Environmental and Occupational Health ,Usability ,General Medicine ,Toxicology ,Decentralization ,Occupational safety and health ,Presentation ,Medicine ,business ,media_common - Published
- 2006
37. Evaluation of Packed Distillation Columns
- Author
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G. H. Sugimura, T. W. Reynolds, and T. J. Walsh
- Subjects
Materials science ,Chromatography ,law ,General Engineering ,Distillation ,law.invention - Published
- 1953
38. Life-structure of the aged
- Author
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H. Sugimura
- Subjects
Gerontology ,Geography ,Structure (category theory) ,Geriatrics and Gerontology - Published
- 1964
39. Recent trends in different histological types of lung cancer in Tokyo based on pathological autopsy records
- Author
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S, Tsugane, S, Watanabe, H, Sugimura, Y, Urano, and S, Matsubara
- Subjects
Adult ,Male ,Lung Neoplasms ,Sex Factors ,Carcinoma, Squamous Cell ,Humans ,Female ,Autopsy ,Adenocarcinoma ,Middle Aged ,Tokyo ,Aged - Abstract
The recently increasing trend of lung cancer mortality in Japan was qualitatively analyzed. As the percentages of cases undergoing pathological autopsies in Tokyo were thirty for males and twenty-six for females during the period from 1979 to 1983, the histologically classified death rates in Tokyo could be estimated by combining the reported cases of death from lung cancer with the proportion of these of known histological type. The results indicated an increase in adenocarcinoma, and a decrease in squamous-cell carcinoma, except for the older age-group of men. The latter result suggested that the results were affected by the decreasing proportion of smokers and by the improvement in cigarette quality. The increase in small-cell and large-cell carcinoma, and the decrease in undifferentiated carcinomas, could be explained by problems associated with differing diagnostic standards. However, when these three different types of carcinoma were considered as a single type, the death rate was shown to be increased except in younger age-groups of women.
- Published
- 1987
40. Stereoselective Synthesis of Spirolactone Analogues of Pyrrolomorpholine Alkaloids.
- Author
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Uchiyama A, Ohta H, Ogawa Y, Sasaki I, and Sugimura H
- Abstract
The synthesis of a spirolactone analogue of xylapyrraside B
1 , a potent antioxidant agent, is described. The key step is the stereoselective formation of the spirolactone skeleton via the formal [3+2] annulation of the isopropylidene-protected glyceraldehyde and δ-methylene lactone, mediated by trifluoroborane etherate. This study addresses the stereoselective synthesis of pyrrolomorpholine spiroketal alkaloids, enabling the production of these bioactive compounds and their analogues.- Published
- 2024
- Full Text
- View/download PDF
41. Bioinformatic Identification of TP53 Gene Mutation Hotspots in Colorectal Cancer.
- Author
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Kovács Z, Sugimura H, György TA, Osvath E, Manirakiza F, and Gurzu S
- Subjects
- Humans, Male, Female, Middle Aged, Aged, Genetic Predisposition to Disease, Colorectal Neoplasms genetics, Colorectal Neoplasms pathology, Computational Biology methods, Mutation, Tumor Suppressor Protein p53 genetics, Exons genetics
- Abstract
Mutations and inactivation of the TP53 gene are frequently observed in various types of malignancies. Precise knowledge of the genetic structure and detection of mutation hotspots are crucial, as these indicate a high probability of developing cancer. The aim of our study was to perform the bioinformatic detection of mutation hotspots in the TP53 gene in patients diagnosed with malignant colon neoplasms using self-developed software (version 1). We compared TP53 gene sequences from 50 healthy individuals with those from 50 patients diagnosed with colorectal carcinoma. Of the 50 samples from cancer patients, the most frequent mutations were observed in exons 5 and 8 (12 mutations per exon) and gene sequences of 12 samples, which differed from those of the 50 samples from healthy individuals. Based on our results, the distribution of mutations in the TP53 gene structure was not even across different exons. By comparing the gene sequences of healthy individuals with those of colon cancer samples, we conclude that structural changes occurring in similar gene regions are not associated with increases in susceptibility to malignancies in every case, namely, that the pathological mechanism is multifactorial.
- Published
- 2024
- Full Text
- View/download PDF
42. Successful Transvenous Implantation of a Permanent Pacemaker in a Patient with Situs Inversus with Dextrocardia Supported by Preceding Three-dimensional Computed Tomography.
- Author
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Nomura A, Otani N, Kokubun A, Mizuguchi S, Kawamoto S, Tomoe T, Kitahara K, Sugiyama T, Horie Y, Sugimura H, and Yasu T
- Subjects
- Humans, Female, Aged, Sick Sinus Syndrome therapy, Sick Sinus Syndrome diagnostic imaging, Sick Sinus Syndrome complications, Prosthesis Implantation methods, Prosthesis Implantation instrumentation, Treatment Outcome, Dextrocardia diagnostic imaging, Dextrocardia complications, Situs Inversus diagnostic imaging, Situs Inversus complications, Pacemaker, Artificial, Imaging, Three-Dimensional, Tomography, X-Ray Computed
- Abstract
We herein report a 76-year-old woman with situs inversus and dextrocardia who underwent pacemaker implantation for sick sinus syndrome. Situs inversus with dextrocardia, which is frequently associated with cardiovascular malformation, is a rare congenital malformation wherein the thoracic and abdominal viscera are inverted compared with their normal positions. This renders the implantation of cardiac devices an arduous task. We therefore decided to gather preoperative anatomical information on patients with situs inversus and dextrocardia. We used three-dimensional computed tomography to collect preoperative information in order to facilitate the safe implantation of cardiac devices.
- Published
- 2024
- Full Text
- View/download PDF
43. Correction: The spectrum of TP53 mutations in Rwandan patients with gastric cancer.
- Author
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Nzitakera A, Surwumwe JB, Ndoricyimpaye EL, Uwamungu S, Uwamariya D, Manirakiza F, Ndayisaba MC, Ntakirutimana G, Seminega B, Dusabejambo V, Rutaganda E, Kamali P, Ngabonziza F, Ishikawa R, Rugwizangoga B, Iwashita Y, Yamada H, Yoshimura K, Sugimura H, and Shinmura K
- Published
- 2024
- Full Text
- View/download PDF
44. The spectrum of TP53 mutations in Rwandan patients with gastric cancer.
- Author
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Nzitakera A, Surwumwe JB, Ndoricyimpaye EL, Uwamungu S, Uwamariya D, Manirakiza F, Ndayisaba MC, Ntakirutimana G, Seminega B, Dusabejambo V, Rutaganda E, Kamali P, Ngabonziza F, Ishikawa R, Rugwizangoga B, Iwashita Y, Yamada H, Yoshimura K, Sugimura H, and Shinmura K
- Abstract
Background: Gastric cancer is the sixth most frequently diagnosed cancer and third in causing cancer-related death globally. The most frequently mutated gene in human cancers is TP53, which plays a pivotal role in cancer initiation and progression. In Africa, particularly in Rwanda, data on TP53 mutations are lacking. Therefore, this study intended to obtain TP53 mutation status in Rwandan patients with gastric cancer., Results: Formalin-fixed paraffin-embedded tissue blocks of 95 Rwandan patients with histopathologically proven gastric carcinoma were obtained from the University Teaching Hospital of Kigali. After DNA extraction, all coding regions of the TP53 gene and the exon-intron boundary region of TP53 were sequenced using the Sanger sequencing. Mutated TP53 were observed in 24 (25.3%) of the 95 cases, and a total of 29 mutations were identified. These TP53 mutations were distributed between exon 4 and 8 and most of them were missense mutations (19/29; 65.5%). Immunohistochemical analysis for TP53 revealed that most of the TP53 missense mutations were associated with TP53 protein accumulation. Among the 29 mutations, one was novel (c.459_477delCGGCACCCGCGTCCGCGCC). This 19-bp deletion mutation in exon 5 caused the production of truncated TP53 protein (p.G154Wfs*10). Regarding the spectrum of TP53 mutations, G:C > A:T at CpG sites was the most prevalent (10/29; 34.5%) and G:C > T:A was the second most prevalent (7/29; 24.1%). Interestingly, when the mutation spectrum of TP53 was compared to three previous TP53 mutational studies on non-Rwandan patients with gastric cancer, G:C > T:A mutations were significantly more frequent in this study than in our previous study (p = 0.013), the TCGA database (p = 0.017), and a previous study on patients from Hong Kong (p = 0.006). Even after correcting for false discovery, statistical significance was observed., Conclusions: Our results suggested that TP53 G:C > T:A transversion mutation in Rwandan patients with gastric cancer is more frequent than in non-Rwandan patients with gastric cancer, indicating at an alternative etiological and carcinogenic progression of gastric cancer in Rwanda., (© 2024. The Author(s).)
- Published
- 2024
- Full Text
- View/download PDF
45. Left Main Bronchus Obstruction in a Patient with Small-cell Lung Cancer Successfully Treated with Venovenous Extracorporeal Membrane Oxygenation.
- Author
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Nagai T, Yoshida K, Otsuki A, So Y, Karumai T, Sugimura H, Tachibana Y, Fukuoka J, Ito H, and Nakashima K
- Subjects
- Humans, Bronchi, Extracorporeal Membrane Oxygenation, Lung Neoplasms complications, Lung Neoplasms therapy, Respiratory Insufficiency etiology, Respiratory Insufficiency therapy, Airway Obstruction therapy, Airway Obstruction complications, Respiratory Distress Syndrome, Small Cell Lung Carcinoma complications, Small Cell Lung Carcinoma therapy
- Abstract
Lung cancer can cause fatal central airway obstruction. Rapid airway clearance is necessary in some cases, but ventilator management may be insufficient to maintain oxygenation levels. Venovenous extracorporeal membrane oxygenation (VV-ECMO) may be an effective rescue therapy for respiratory failure, but its efficacy in treating tumor-related airway obstruction is unknown. We herein report a case of central airway obstruction and severe acute respiratory failure due to small-cell lung cancer successfully treated with VV-ECMO, bronchoscopic airway intervention, and chemotherapy. VV-ECMO can be an effective option for the treatment of central airway obstruction with acute respiratory failure due to lung cancer.
- Published
- 2024
- Full Text
- View/download PDF
46. Relationship Between Increased Oxygen Uptake and Lactate Production With Progressive Incremental Electrode Skeletal Muscle Stimulation: A Pilot Study.
- Author
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Tamura Y, Ochiai K, Takahashi M, Takahashi H, Tomoe T, Sugiyama T, Otani N, Sugimura H, Toyoda S, and Yasu T
- Abstract
Background Belt electrode skeletal muscle stimulation (B-SES) is an alternative exercise therapy for those with difficulty performing voluntary exercise. However, it is unknown whether oxygen uptake (VO
2 ) in B-SES is comparable to cardiopulmonary exercise test (CPX) as assessed by voluntary exercise. This study aimed to evaluate oxygen uptake (VO2 ) and lactate (LA) production in incremental B-SES compared to ergometer CPX and to determine the relationship with ergometer CPX. Methods This study included 10 healthy young Japanese participants. Using a crossover design, all participants underwent incremental B-SES CPX and ergometer CPX using a 20 W ramp. Serum lactic acid concentration (LA) was measured serially before, during, and after B-SES. The tolerability of B-SES was adjusted with the change in LA level (⊿LA). Results Peak VO2 during B-SES (14.1±3.3 mL/kg/min) was significantly lower than ergometer peak VO2 (30.2±6.2 mL/kg/min, P<0.001). B-SES peak VO2 was similar to the anaerobic threshold (AT) VO2 on ergometer CPX (15.1±2.6 mL/kg/min). LA (Rest: 1.4±0.3, Peak: 2.8±0.8 mmol) and plasma noradrenalin (Rest: 0.2±0.1, Peak: 0.4±0.1 ng/mL) levels increased after B-SES. No significant correlation was observed between B-SES peak VO2 and ergometer CPX. However, after adjusting for B-SES, tolerability, it (peak VO2 of B-SES /⊿LA) correlated with peak VO2 (r=0.688, p=0.028) on the ergometer. Conclusion Peak VO2 of the passively progressive B-SES almost reached the AT value of the ergometer CPX without adverse events. Peak VO2 of B-SES adjusted with ⊿LA may be used to predict peak VO2 in ergometer CPX., Competing Interests: The authors have declared financial relationships, which are detailed in the next section., (Copyright © 2024, Tamura et al.)- Published
- 2024
- Full Text
- View/download PDF
47. Risk Evaluation of Proton Pump Inhibitors for Panitumumab-Related Hypomagnesemia in Patients with Metastatic Colorectal Cancer.
- Author
-
Katsura H, Suga Y, Kubo A, Sugimura H, Kumatani K, Haruki K, Yonezawa M, Narita A, Ishijima R, Ikesue H, Toi H, and Takata N
- Subjects
- Humans, Panitumumab adverse effects, Proton Pump Inhibitors adverse effects, Retrospective Studies, Magnesium therapeutic use, Colonic Neoplasms drug therapy, Colorectal Neoplasms pathology
- Abstract
Hypomagnesemia commonly occurs as a side effect of panitumumab treatment. In severe cases, temporary discontinuation or dose reduction of panitumumab may be necessary. Proton pump inhibitors (PPIs) are reportedly potential risk factors for hypomagnesemia. We conducted a multicenter study to assess the impact of PPIs on the risk of grade 3-4 hypomagnesemia in patients with metastatic colorectal cancer (mCRC) receiving panitumumab. We adjusted for potential bias using a propensity score-matched analysis and retrospectively reviewed the medical records of patients. Hypomagnesemia severity was graded according to the Common Terminology Criteria for Adverse Events, version 5.0. A total of 165 patients were enrolled in this study. The incidence of grade 3-4 hypomagnesemia was significantly higher in the PPI group than in the non-PPI group, both before (20.0% [30/60] vs. 8.0% [8/105], p = 0.026) and after propensity score matching (16.2% [6/37] vs. 0% [0/37], p = 0.025). In the propensity score-matched cohort, the risk of grade 3-4 hypomagnesemia was significantly higher in the PPI group (odds ratio, 2.19; 95% confidence interval, 1.69-2.84; p = 0.025). These findings suggest that concomitant use of PPIs significantly increases the risk of grade 3-4 hypomagnesemia in patients with mCRC receiving panitumumab. Therefore, close monitoring of these patients is imperative.
- Published
- 2024
- Full Text
- View/download PDF
48. DNA methylation status of SHATI/NAT8L promoter in the blood of cigarette smokers.
- Author
-
Izuo N, Miyanishi H, Nishizawa D, Fujii T, Hasegawa J, Sato N, Tanioka F, Sugimura H, Ikeda K, and Nitta A
- Subjects
- Humans, DNA Methylation, Smokers, Tobacco Use Cessation Devices, Biomarkers, Acetyltransferases metabolism, Smoking Cessation, Tobacco Products
- Abstract
Aims: Cigarette smoking is a preventable risk factor for various diseases such as cancer, ischemic stroke, cardiac stroke, and chronic obstructive pulmonary disease. Smoking cessation is of great importance not only for individual smokers but also for social health. Regarding current cessation therapies, the effectiveness of nicotine replacement is limited, and the cost of varenicline medication is considerable. Thus, a method for screening smokers who are responsive to cessation therapy based on the therapeutic effectiveness is required. Peripheral biomarkers reflecting smoking dependence status are necessary to establish a method for achieving effective cessation therapy., Methods: Methylation status of smokers' blood DNA was evaluated focusing on SHATI/NAT8L, an addiction-related gene. Eight CpG sites in SHATI/NAT8L were quantified by pyrosequencing., Results: There was no difference in the methylation status of this gene between smokers (n = 129) and non-smokers (n = 129) at all CpG sites. No correlations between the methylation status of SHATI/NAT8L and indicators of smoking dependence were found., Conclusions: Although the present study found no significance in the DNA methylation of SHATI/NAT8L among smokers, the exploration of predictable peripheral biomarkers for the effectiveness of smoking cessation therapy is required., (© 2023 The Authors. Neuropsychopharmacology Reports published by John Wiley & Sons Australia, Ltd on behalf of The Japanese Society of Neuropsychopharmacology.)
- Published
- 2023
- Full Text
- View/download PDF
49. High Expression of Fas-Associated Factor 1 Indicates a Poor Prognosis in Non-Small-Cell Lung Cancer.
- Author
-
Hu, Yamada H, Yoshimura K, Ohta T, Tsuchiya K, Inoue Y, Funai K, Suda T, Iwashita Y, Watanabe T, Ogawa H, Kurono N, Shinmura K, and Sugimura H
- Subjects
- Humans, Adaptor Proteins, Signal Transducing genetics, Adaptor Proteins, Signal Transducing metabolism, Carrier Proteins genetics, Carrier Proteins metabolism, Fibrinogen, Prognosis, Apoptosis Regulatory Proteins genetics, Carcinoma, Non-Small-Cell Lung genetics, Lung Neoplasms genetics, Lung Neoplasms metabolism
- Abstract
Fas-associated factor 1 (FAF1) is a death-promoting protein identified as an interaction partner of the death receptor Fas. The downregulation and mutation of FAF1 have been reported in a variety of human tumors, but there have been few studies on lung cancer. Here, we investigated the prognostic significance of FAF1 expression in non-small-cell lung cancer (NSCLC), and whether aberrant FAF1 expression may be involved in the pathogenesis and prognosis of NSCLC. FAF1 expression was examined in NSCLC specimens as well as human lung cancer cell lines. In addition, changes in cell viability and apoptosis upon regulating FAF1 expression were investigated in lung cancer cell lines. As a result, high FAF1 expression was significantly associated with a poor prognosis in NSCLC. In lung cancer cell lines, FAF1 downregulation hindered cell viability and tended to promote early apoptosis. In conclusion, this is the first study of the clinical significance of FAF1 in NSCLC, showing that FAF1 overexpression is associated with a poor prognosis in NSCLC and that FAF1 acts as a dangerous factor rather than an apoptosis promoter in NSCLC.
- Published
- 2023
- Full Text
- View/download PDF
50. Interaction between the substrate and probe in liquid metal Ga: experimental and theoretical analysis.
- Author
-
Amano KI, Tozawa K, Tomita M, Takagi R, Iwayasu R, Nakano H, Murata M, Abe Y, Utsunomiya T, Sugimura H, and Ichii T
- Abstract
Interaction between two bodies in a liquid metal is an important topic for development of metallic products with high performance. We conducted atomic force microscopy measurements and achieved the interaction between the substrate and the probe in liquid Ga of an opaque and highly viscous liquid. The interaction cannot be accessed with the normal atomic force microscopy, electron microscopy, and beam reflectometry. We performed a theoretical calculation using statistical mechanics of simple liquids by mixing an experimentally derived quantum effect. From both experiment and theory, we found an unusual behaviour in the interaction between the solvophobic substances, which has never been reported in water and ionic liquids. Shapes of the interaction curves between several solvophobic and solvophilic pairs in liquid Ga are also studied., Competing Interests: There are no conflicts of interest to declare., (This journal is © The Royal Society of Chemistry.)
- Published
- 2023
- Full Text
- View/download PDF
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