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Touch DNA analysis has become an important aspect of a forensic laboratory’s workload and a crucial tool for investigators in many cases. However, there is a lack of research regarding the influence of environmental conditions on Touch DNA, which is proven to reduce traces of biological material in samples. This study investigated the influence of time between deposition and recovery of Touch DNA, as well as the impact of temperature and humidity on a range of porous and non-porous surfaces.

There are different variables that affect the success of Touch DNA recovery, including surface type, the collection method used and extraction techniques. This experiment investigated how a range of porous and non-porous surfaces, different DNA collection (cotton swab, nylon flocked swab and SceneSafe Fast™ minitape) and extraction methods (PrepFiler Express BTA™ and QIAamp® DNA Investigator) affected touch DNA recovery.

Touch DNA Profiling is a commonly used tool to link suspects to their crimes, but it is often challenging compared to other types of DNA evidence, such as blood and saliva, because it is usually found in minute quantities. Using direct amplification techniques to avoid sample extraction and quantification during the DNA profiling process can help preserve DNA loss. Therefore, this study explored an innovative solution to collect blood, saliva and Touch DNA using a cotton swab in combination with a microFLOQ® swab (CS+MF) for direct amplification to preserve the collected samples for re-analysis or additional testing. The allele recovery rate was 100% for blood and saliva samples, and 84% for trace samples, with a considerable difference in the average signal (RFU) between the evidence types (p < 0.001).

Trace DNA is commonly found at crime scenes and is often collected from the victim’s clothing in cases of sexual assault, homicide, and theft, typically by tape lifting. This study investigated the influence of area size and fabric type on the Touch DNA collected from fabric. The amount of collected Touch DNA from the fabric was significantly affected by fabric type (p < 0.05) and the interaction between fabric size and collection type (p < 0.05). More trace DNA was recovered from fabric containing a high percentage of polyester than 100% woven cotton. Minitapes were very effective for sampling Touch DNA from small areas of fabric but equally effective to cotton swabs for larger areas.

Trace DNA, widely known as Touch DNA, is a type of DNA evidence commonly found at crime scenes, and it is frequently used to link suspects to crimes committed but it is a more challenging type of DNA evidence compared to other biological samples. This study investigated the influence of collection method and extraction type on Touch DNA collected from fabric. The amount of collected Touch DNA from the fabric was significantly affected by collection type (p < 0.05), with Scene Safe Fast™ minitape (K545) being more efficient than Copan cotton swab (150C) and Copan nylon flocked swab (4N6FLOQSwabs®) on recovering touch DNA from fabric.

Aims/Introduction Abnormalities in Ca2+ signaling have a key role in hemodynamic dysfunction in diabetic heart. The purpose of this study was to explore the effects of streptozotocin (STZ)‐induced diabetes on Ca2+ signaling in epicardial (EPI) and endocardial (ENDO) cells of the left ventricle after 5–6 months of STZ injection. Materials and Methods Whole‐cell patch clamp was used to measure the L‐type Ca2+ channel (LTCC) and Na+/Ca2+ exchanger currents. Fluorescence photometry techniques were used to measure intracellular free Ca2+ concentration. Results Although the LTCC current was not significantly altered, the amplitude of Ca2+ transients increased significantly in EPI‐STZ and ENDO‐STZ compared with controls. Time to peak LTCC current, time to peak Ca2+ transient, time to half decay of LTCC current and time to half decay of Ca2+ transients were not significantly changed in EPI‐STZ and ENDO‐STZ myocytes compared with controls. The Na+/Ca2+ exchanger current was significantly smaller in EPI‐STZ and in ENDO‐STZ compared with controls. Conclusions STZ‐induced diabetes resulted in an increase in amplitude of Ca2+ transients in EPI and ENDO myocytes that was independent of the LTCC current. Such an effect can be attributed, at least in part, to the dysfunction of the Na+/Ca2+ exchanger. Additional studies are warranted to improve our understanding of the regional impact of diabetes on Ca2+ signaling, which will facilitate the discovery of new targeted treatments for diabetic cardiomyopathy., This is the first report to investigate the effects of streptozotocin‐induced diabetes on L‐type Ca2+ current simultaneously measured with Ca2+ transients in epicardial and endocardial left ventricular myocytes isolated from rats 5–6 months after the induction of diabetes. Streptozotocin‐induced diabetes causes a progressive increase in the amplitude of endocardial and epicardial myocyte Ca2+ transients that is independent of the L‐type Ca2+ current. The effect of streptozotocin‐induced diabetes on Ca2+ handling might be attributed, at least in part, to the dysfunction of the Na+/Ca2+ exchanger.

Purpose of the state-of-the-art review: Left ventricular (LV) global longitudinal strain (GLS) is recently recognized as a more sensitive measure of LV myocardial systolic function compared with LV ejection fraction (LVEF). In addition, left ventricular GLS , myocardial performance index (MPI) and maximum rate of LV pressure rise during isovolumetric contraction (LV dP/dtmax) are more reproducible than traditional assessment of LV systolic function by two dimensional echocardiography (2DE) LVEF. These underutilized techniques can detect preclinical myocardial dysfunction in patients who are at risk of LV failure in valvular-induced heart disease . Current guidelines for diagnosis and treatment of valvular heart disease (VHD) include LVEF as one of the parameters to take into consideration in the clinical decision-making. However, a large body of evidence has shown that left ventricular GLS, MPI and LV dP/dtmax have been classically considered as a sensitive marker of LV contractility and inotropic state. In turn GLS and myocardial performance may be a better prognosticator than LVEF in aortic and mitral valve heart diseases. This timely state-of-the-art review, appraised the evidence and role of GLS, MPI and dP/dT as clinical tools in patients with aortic and mitral valve disease. Recent findings: Left ventricular GLS has been shown to be prognostic in low-flow, low-gradient severe aortic stenosis with preserved LVEF. The role of left ventricular GLS, Tei index (MPI) and maximum rate of LV pressure rise (LV dP/dtmax) in patients with aortic regurgitation and mitral valve diseases (regurgitation and stenosis) is less well established. Summary: Echocardiography is considered the primary non-invasive imaging tool for valvular heart disease assessment and the cornerstone method in diagnosing and evaluating the morphology and severity of aortic and mitral valve diseases. Currently, diagnostic-cardiac catheterization is no more recommended except in very rare cases when echocardiographic image quality is suboptimal, non-diagnostic and when the results of 2DE are discrepant with clinical data. Once clinical decision-making is based on the 2DE and three dimensional echocardiographic in assessment of the severity of mitral and aortic valve diseases, it is crucial that standards should be adopted to maintain accuracy and consistency across echocardiographic laboratories. This illustrative review article assesses left ventricular systolic function (LVEF) employing two and/or three dimensional echocardiography in comparison to GLS, MPI and LV dP/dtmax, especially applied for aortic valve (AV) and mitral valve (MV) diseases. It is noteworthy that this document only provides echocardiographic standards rather than making recommendations for clinical management. Conclusion: It is concluded that GLS, MPI and maximum rate of LV pressure rise during isovolumetric contraction (LV dP/dtmax) are recommended and more so, they should be increasingly used to identify subclinical LV myocardial dysfunction in patients with mitral and aortic valve heart diseases, to identify optimal timing for surgery and prognosticate outcomes after surgery

The rapid wide-scale spread of fall armyworm (Spodoptera frugiperda) has caused serious crop losses34 globally. However, differences in the genetic background of subpopulations and the mechanisms of rapid adaptation behind the invasion are still not well understood. Here we report the assembly of a 390.38-M chromosome-level genome of fall armyworm using Pacific Bioscience (PacBio) and Hi-C sequencing technologies with scaffold N50 of 12.7 M consisting of 22260 annotated protein-coding genes. Genome-wide resequencing of 103 samples from 16 provinces in China revealed that the fall armyworm population comprises a complex inter-strain hybrid, mainly with the corn-strain genetic background and less of the rice-strain, which highlights the inaccuracy of strain identification using mitochondrial or Triosephosphate isomerase (Tpi) genes. Analysis of genes related to pesticide- and Bt-resistance showed that the risk of fall armyworm developing resistance to conventional pesticides is very high. Laboratory bioassay results showed that insects invading China carry resistance to organophosphate and pyrethroid pesticides, but are sensitive to genetically modified maize expressing Bacillus thuringiensis (Bt) toxins Cry1Ab in field experiments. Additionally, two mitochondrial fragments are inserted into the nuclear genome, and the insertion event occurred after the differentiation of the two strains. This study represents a valuable advance toward improving management strategies for fall armyworm.

Offender profiling follows the idea that if offenders’ crime scene actions can be empirically linked to their background characteristics, it will be possible to predict one from the other. There is a lack of research exploring whether homicide offenders’ crime scene actions are predictive of their criminal histories, despite the potential utility of such information. The current study addresses this gap in the literature. A sample of 213 adult male-on-female homicides with sexual or unknown motive was drawn from a U.K.-wide database. Relationships between 13 preconviction variables and 29 crime scene behaviors were explored using a bivariate statistical approach. Subsequently, binary logistic regression models were used to predict the presence, or absence, of specific preconvictions based on a combination of offense behaviors. Analyses highlighted 16 statistically significant associations between key offense behaviors and previous convictions, these associations were often “less likely” to result in previous conviction. The analysis failed to find any association for various other variables, most notably sexual preconvictions. Results indicate offenders’ criminal histories can be predicted from their offense behaviors, though not all preconvictions may be similarly suited. Implications for practice are discussed.

Traditional medicines, derived from plants, could present alternative treatment strategy for cancer therapy. One such plant is Momordica charantia (MC) which possesses anti-carcinogenic properties. This study investigated the anticancer effect of an ethanol extract of MC fruit, Kuguacin-J (K-J), an isolated compound from the leaves of MC and cisplatin, either alone or combination on healthy MCF-10A mammary cells and compared with breast cancer MCF-7 and MDAMB-231 cell lines. Cell viability was tested using 8 μg/mL and 80 μg/mL doses of MC extract, K-J and cisplatin individually or combined for 24 and 48 hours. Caspase-3- activity was measured in MCF-7 and MDA-MB-231 cells using established methods. The results revealed that MC extract and K-J had no effect on healthy MCF-10A cell viability as compared to cisplatin which induced dose and time-dependent cell death. Similarly, treatment of MCF-7 cells with cisplatin induced cell death at high concentration at both the time points, while MC extract and K-J only induce MCF-7 cell death at high dose after 48 hours only. During combination therapy, both doses of cisplatin enhanced MCF-7 cell death when combined with MC extract or K-J after 24 and 48 hours. In MDAMB-231 cells, the three drugs, either alone or combined, evoked significant cell death at both the doses and time points. All three drugs, at high dose, elicited significant increase in caspase-3- activity in MCF-7 and MDA-MB-231 cells as compared to untreated cells. The results revealed that either MC extract or K-J alone or combined with cisplatin, can elicit significant increase in cell death and caspase–3-activity in MCF-7 and MDA-MB-231cells as compared to untreated cells.

For approximately two decades, Microsatellites or Short Tandem Repeat (STR) markers have been the backbone for human identification testing in the forensic field. The expansion of STR multiplex kits can improve global sharing of STR profiles, rapid DNA typing, and DNA typing Flores et al. (2014). GlobalFiler™ kit is highly sensitive and has extremely high power of discrimination. An internal validation of GlobalFiler™ Kit was carried out to test the robustness of this kit through a range of internal validation studies including half volume reaction, reproducibility, sensitivity, specificity, stability, mixture, analytical threshold, sensitivity & stochastic threshold, heterozygous balance & stutter threshold studies in accordance with SGWDAM guidelines Scientific Working Group on DNA . Reference and real casework samples previously tested using other autosomal STR kits from forensic cases were used during the validation. It was demonstrated that the GlobalFiler™ kit has enhanced discrimination power, is robust and extremely useful for forensic casework.

Short tandem repeat (STR) profiling has been routinely used in kinship testing since the introduction of commercial kits in the mid-1990s. While 15 to 23 STR loci normally give definitive results in simple kinship testing, additional loci are sometimes required to resolve complex cases. The SureID 23comp Human Identification Kit, recently released by Health Gene Technologies (China), multiplexes amelogenin and 22 autosomal STRs, 17 of which are non-CODIS STRs. This enables the profiling of 38–40 loci when used in conjunction with widely used commercial kits. In this study, the kit was evaluated for kinship applications as a supplementary STR kit following the minimum criteria for validation recommended by the European Network of Forensic Science Institutes (ENFSI) and the Scientific Working Group on DNA Analysis Methods (SWGDAM) using 500 samples. Performance was comparable with other commercial kits demonstrating: repeatability and reproducibility; precision (maximum s.d. 0.1048 nt); accuracy, all alleles were within ±0.41 nt compared to the actual sizes; heterozygous peak balances at all loci >68%; stutter ratios ranged from 3.8% to 16.15%; full profiles were generated with 125 pg DNA (95.12% of alleles at 62 pg),; and we found 100% concordance over 5 common STRs with the GlobalFiler kit.

Nutrition is vital to health and the availability of resources has long been acknowledged as a key factor in the ability to fight off parasites, as investing in the immune system is costly. Resources have typically been considered as something of a “black box”, with the quantity of available food being used as a proxy for resource limitation. However, food is a complex mixture of macro- and micronutrients, the precise balance of which determines an animal's fitness. Here we use a state-space modelling approach, the Geometric Framework for Nutrition (GFN), to assess for the first time, how the balance and amount of nutrients affects an animal's ability to mount an immune response to a pathogenic infection. Spodoptera littoralis caterpillars were assigned to one of 20 diets that varied in the ratio of macronutrients (protein and carbohydrate) and their calorie content to cover a large region of nutrient space. Caterpillars were then handled or injected with either live or dead Xenorhabdus nematophila bacterial cells. The expression of nine genes (5 immune, 4 non-immune) was measured 20 h post immune challenge. For two of the immune genes (PPO and Lysozyme) we also measured the relevant functional immune response in the hemolymph. Gene expression and functional immune responses were then mapped against nutritional intake. The expression of all immune genes was up-regulated by injection with dead bacteria, but only those in the IMD pathway (Moricin and Relish) were substantially up-regulated by both dead and live bacterial challenge. Functional immune responses increased with the protein content of the diet but the expression of immune genes was much less predictable. Our results indicate that diet does play an important role in the ability of an animal to mount an adequate immune response, with the availability of protein being the most important predictor of the functional (physiological) immune response. Importantly, however, immune gene expression responds quite differently to functional immunity and we would caution against using gene expression as a proxy for immune investment, as it is unlikely to be reliable indicator of the immune response, except under specific dietary conditions., Graphical abstract Image 1, Highlights • High protein diets improved survival after live bacterial infection. • Injection with dead bacteria increased expression of Toll and IMD immune genes. • Injection with live bacteria inhibited immune gene expression (GE). • The ratio and concentration of macronutrients in the diet affected GE. • GE only predicted functional immune activity at high levels of dietary protein.

Rilpivirine is a non-nucleoside reverse transcriptase inhibitor, recently developed as a drug of choice for initial anti-retroviral (ARV) treatment of HIV-1 infection, whereas estradiol is a major component of hormonal contraceptives. Both drugs have effects on lipid metabolism, impairment of adipocyte differentiation and alteration of adipose tissue distribution and function.This study investigated the effects of different concentrations of either rilpivirine or estradiol either alone or in combination on adipocyte differentiation and adipocytokines status in vitroin the absence and presence of β-naphthoflavone, (BNF),a potent agonist of the aryl hydrocarbon receptor. 3T3-L1 human pre-adipocytes were cultured and differentiated with different concentrations of treatment drugs. After 10 days of differentiation procedure, cells were examined for their morphology and viability. Glycerol,adiponectin, leptin, resistin and interleukin-8 (IL-8) were quantified using commercially available kits. The results show that either rilpivirine or estradiol individually or during their combination can evoke significant increases in glycerol release and a concomitant significant decrease of adiponectin from adipocytes. These effects were dose–dependent. The effects of combined treatments were much larger than individual concentration for each drug. Both drugs had little of no effect on leptin levels, except for a small decrease with 10 µM rilpivirine alone or when combined with estradiol. In addition, both drugs evoked small increases in the release of resistin and interleukin-8 with significant values at higher doses compared to untreated adipocytes.When adipocytes were pretreated with BNF, either rilpivirine or , estradiol or when combined evoked a much larger release in glycerol and a much larger decrease in adiponectin compared to the absence of BNF. In contrast, BNF pretreatment had little of no effect on either leptin, resistin or IL-8 metabolism compared to the results obtained in the presence of either rilpivirine or estradiol alone or in combination.These results show that rilpivirine and estradiol either alone or when combined or pretreated with BNF can evoke marked effects on glycerol and cytokines levels from adipocytes. However, their mechanism (s) in inducing adipogenesis warrants further investigation of different transcription factors at gene expression levels.

Touch DNA, commonly known as trace DNA, is widely used in forensic DNA casework. However, touch DNA samples are challenging because of the many variables that can impact the success of obtaining a good quality usable DNA profile. Cotton and nylon flocked swabs are often used to collect touch DNA from surfaces, so this study aimed to test different techniques using cotton (150C) and nylon flocked (4N6FLOQSwabs®) swabs to collect touch DNA from non-porous surfaces. There was a significant difference amongst the three recovery techniques tested to recover touch DNA with cotton swabs and nylon swabs from textured plastic (p < 0.001), with a nylon swab and 30μl of distilled water being more efficient than a cotton swab with 100μl of distilled water. There was also a significant difference between the four recovery techniques to recover touch DNA from glass surfaces exposed to high humidity and low temperature (5 °C/78%) (p

The effective search for the missing and identification of persons, alive or dead, are core components in the prevention and in resolving the issue of Missing Persons. Despite the growing literature on this topic, there is still a lack of publications describing the Search as a process that includes different phases inherently composed of forensic investigative and identification principles for both living and deceased missing persons. This paper is the result of discussions between the Forensic Unit of the International Committee of the Red Cross (ICRC) and members of its external Forensic Advisory Board. It aims to present the Search process as an overarching concept that includes the investigation and identification phases of the missing in any state (dead or alive), in any scenario (with or without bodies), with an integrated, multidisciplinary, and multiagency approach for implementation by all actors involved in the investigation and identification phases of missing persons. [Abstract copyright: © 2021 The Authors.]

Prayer, meditation and mindfulness have been commonly used for healing in Vedic, Jainism, Buddhism, Egyptian, Greek and Mesopotemian cultures [1]. In Buddhism, mindfulness is a component of Eight Fold Path, therefore using mindfulness in the modern science is not absolutely correct. A definition of prayers of Mesopotamia was “praise to god followed by request.” The ancient mystic Hinduism wrote Rigveda prayer, Figure 1.\ud Meditation and prayer appear to be important practices for the management of emotional and spiritual wellbeing [1-4]. Prayer may also influence the physiology of circadian rhythms in our body and it has evolved possibly as adaptation to the rotation of the earth around its axis [4]. Circadian rhythms account for increased activity of the sympathetic nerves of the autonomic nervous system with marked releases of cortisol, catecholamines (norepinephrine and adrenaline), testosterone and thyroid hormones. In turn, these neurotransmitters and hormones occur at a circadian stage when endogenous melatonin level is decreased to low values in the morning [4]. There is a need to prevent the circadian rhythm of adverse biological functions observed between 6.00 to 12.00 hours during the day, because circadian dysfunction can predispose to cardiovascular diseases (CVDs). The circadian dysfunction may worsen due to risk factors, although protective factors such as prayer, meditation and moderate physical activity may prevent circadian dysfunction [2-4]. The aim of the present study is to emphasize the beneficial role of prayer, mindfulness and meditation in the protection against circadian dysregulation in the body, thereby preventing CVDs, especially sudden cardiac death (SCD).

Circadian rhythm of increased oxidative stress and deficiency of antioxidant vitamins is known to occur in the morning at 6.00 hours to 12.00 hours [1]. Apart from antioxidant and vitamin deficiency, there is also deficiency of catalase, super-oxide-dismutase (SDO) and glutathione peroxidase (GPO) due to increased requirement of antioxidant enzymes in the morning. Methylglyoxal (MGO) is a non-enzymatic metabolite in the glycolytic pathway and its concentration in blood and tissues is elevated in all the cardio-metabolic diseases, including obesity and diabetes mellitus (DM) [1, 2]. MGO is a reactive carbonyl species (RCS) and in turn it induces tissue injuries via reactive oxygen species (ROS), resulting in increased damage of target tissues, which may worsen in the morning. MGO also plays an important pathological role in the development of both cardiovascular diseases (CVDs) and diabetes via the AGEs exert irreversible effects on protein structure and function. As such, it is crucial to understand the underlying mechanism whereby MGO is inducing tissue damage. This mini-review aims to highlight the role of circadian dysfunction in the pathogenesis of diabetes mellitus and its complications.

Touch or trace DNA analysis can be used to connect suspects to crime scenes, especially when other types of biological evidence are unavailable. However, Touch DNA profiling is a challenging process and many variables affect the success of obtaining a good quality DNA profile, such as surface type and the time between deposition and recovery. This study aimed to investigate the influence of time between deposition and recovery of Touch DNA from banana skin surface at room temperature. The results showed that the amount of recovered DNA from the banana skin was not affected by time (over a week) at room temperature and a full DNA profile was generated even when the banana skin was rotten.

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that causes coronavirus disease 2019 (COVID-19) has infected more than 42.5 million people globally resulting in the death of over 1.15 million subjects. It has inflicted severe public health and economic hardships across the world. In addition to acute respiratory distress syndrome, respiratory failure, sepsis, and acute kidney injury, COVID-19 also causes heart failure (HF). COVID-19-induced HF is manifested via different mechanisms, including, but not limited to, (1) virus-induced infiltration of inflammatory cells, which could impair the function of the heart; (2) pro-inflammatory cytokines (monocyte chemoattractant protein-1, interleukin-1β; interleukin-6; tumor necrosis factor-α) that could cause necrosis and death of the myocardium; (3) endothelial injury coupled with micro-thrombosis which could damage the endocardium; and (4) acute respiratory distress syndrome and respiratory failure that could lead to heart failure due to severe hypoxia. It is concluded that the etiology of COVID-19-induced HF is multifactorial and mitigation of the development of HF in patients with COVID-19 will require different approaches such as social distancing, drug therapy, and the urgent development of a vaccine to eradicate the disease.

Background. Obesity and type 2 diabetes mellitus (T2DM) are two interrelated and preventable disorders. However, they are responsible for significant global mortality from cardiovascular diseases (CVDs). Clinical studies have demonstrated that global longitudinal strain (GLS) using speckle tracking echocardiography (STE), can assess myocardial function accurately in apparently, healthy patients with diabetes and obesity in the settings of acute and chronic ischemia and suspected cardiomyopathy without heart failure. No such studies have been published to date regarding subclinical detection of cardiac dysfunction among obese patients with T2DM. This study aims to investigate the role of STE in the early pre-clinical diagnosis of impairment of diastolic and systolic dysfunction in obese patients with T2DM. This study also investigated whether it is possible to detect early pre-clinical impairment of diastolic and systolic dysfunction in obese T2DM patients, via Tissue Doppler Imaging (TDI), maximum rate of left ventricular pressure development (peak dP/dt) and GLS using STE for comparison.\ud Subjects and Methods. After clearance from the review board of Dibba- Hospital, Alfujairah, UAE, all the available records of patients with the diagnosis of obesity and diabetes were examined. The study included 214 patients presenting with obesity in conjunction with diabetes and 93 age-matched healthy control subjects. STE was performed among all the patients and subjects along with Tissue Doppler Imaging (TDI). This study assessed maximal rate of pressure rise during ventricular contraction (peak dP/dt) and global longitudinal strain (GLS) using STE methods. Transthoracic echocardiography, myocardial Doppler-derived systolic (sm), early diastolic velocity (em) and GLS were also obtained, among all the subjects.\ud Results. The results show that cardiac functions via conventional echocardiography (CE) were similar in the 2 groups. Using TDI and conventional mitral Doppler flow, obese subjects with diabetes showed an evidence of diastolic function abnormalities in the form of lower Ea velocity (9.5 ± 2.9 vs. 18.4 ± 3.5 cm/s, p < 0.0001), an increased Aa velocity (16.5 ± 2.4 vs. 14.1 ± 2.2 cm/s p < 0.05), higher left ventricular filling pressure (E/Ea = \ud 12 ± 4.4 vs 8±3.1), p < 0.05), as well as a reduced Ea/Aa ratio (1.00 ± 0.2 vs. 1.45 ± 0.3, p < 0.0001, in the study group versus control group. respectively. This study also showed that severely obese subjects (BMI >35) (n = 26) had reduced LV systolic and diastolic function compared with healthy controls. Regarding, systolic function indices, the findings revealed lower average longitudinal peak systolic strain, sm and reduced em, although, LV ejection fraction remained normal (56.48% ± 8.81). Among subjects with severe obesity (n = 26), the findings reveal that global longitudinal presystolic strain (GLPSS) is highly correlated with maximum rate of pressure development of LV (dp/dt), although the LVEF remained normal, in comparison to systolic strain and dp/dt. (.5% ±1.4 vs -19.54% ± 4.5; \ud p < 0.001), in the age-matched healthy subjects. However, the frequency/grade of DCM detected by STE, among patients having obesity with T2DM, correlated closely with the degree of obesity, metabolic abnormalities and clustering of other major risk factors, especially high blood pressure. The findings also revealed that chest pain due to coronary heart disease (CAD), dyspnea and DCM were more common among female patients compared to men.\ud Conclusion. The results indicate that patients having obesity with T2DM should be advised to undertake early TDI and STE for early diagnosis of decreased cardiac diastolic and systolic dysfunctions and cardiomyopathy, which is likely to be missed by conventional echocardiography. Significant differences in regional and global strain were also identified between the severely obese diabetic (BMI ≥ 35), (GLPSS (-13) patients compared to less obese subjects.

Background: Echocardiography is important in assessing left ventricular mechanical dyssynchrony (LVMD) and left\ud ventricular (LV) function after implant of a permanent pacemaker. Global longitudinal strain (GLS) and Left ventricular dyssynchrony assessment enable clinicians to detect early signs of LV dysfunction after cardiac pacing. This study assessed LV mechanical asynchrony and LV function in different pacing modes after permanent cardiac pacing by using tissue Doppler imaging (TDI) and speckle tracking echocardiography (STE).\ud Patients and Methods: Seventy female (42) and male (28) patients were enrolled in this prospective observational case study (mean age 60.99 ± 13.77 years) at Mansoura Specialized Medical Hospital over a period of 1 year from April 2018 to April 2019. All the patients were assessed by thorough history taking, clinical examination, conventional Echocardiography, TDI and two-dimensional (2D) STE.\ud Results: Regarding parameters assessed by STE, there was a significant decrease in global longitudinal strain GLS (P = 0.034*) while there was a significant increase in time to peak strain standard deviation (TP-SD) by STE (P < 0.001*). Also, there was significant decrease in GLS (P < 0.001*) and significant increase in TP-SD by STE (P = 0.001*) in dual chamber pacemaker (DDD) group. Similarly, there was a significant decrease in GLS (P < 0.001*) and a significant increase in TP-SD by STE (P < 0.001*) in ventricular demanding pacing (VVI) group.\ud Conclusion: The results show that GLS by 2D STE can help in detection of subclinical left ventricular dysfunction (LVD) after permanent pacemaker implantation before appearance of clinical symptoms. In addition, cardiac pacing can still lead to LVMD irrespective of the degree of the pacing mode. However, this may need upgrading later on to cardiac resynchronization therapy (CRT).

In the context of increasing biomass cultivation for energy production in Europe, the objective of this study was to carry out a multi-parameter assessment of soil quality under Miscanthus x giganteus crop at marginal sites. Chemical (pH, organic carbon, total nitrogen, C:N, metal trace elements), biological (microbial biomass, earthworm communities) and physical (aggregate stability, bulk density) soil properties were evaluated 5 years after planting Miscanthus on a polluted (CH) and unpolluted (MG) marginal sites and compared with adjacent undisturbed meadow areas and Miscanthus crop cultivated on an arable field (BF). The effect of Miscanthus on soil quality was site dependent and related to soil properties (texture), metal trace element (MTE) contamination and previous land use. At MG, where Miscanthus was cultivated on a previously undisturbed meadow, results suggested a negative effect of Miscanthus on soil biological properties with a lower earthworm abundance and biomass, and a worst functional and species structure in the Miscanthus than in the undisturbed meadow, despite a good yield. Results under Miscanthus at BF were similar in terms of soil quality to Miscanthus at MG and higher than under Miscanthus at the polluted site (CH). At CH, results suggested that Miscanthus may reduce the mobility of MTE, however further longer term studies, at a range of sites are needed to conclude about the impact of Miscanthus on soil quality at contaminated and/or uncontaminated sites.

Introduction: The increasing prevalence of heart failure (HF) in coronary artery disease (CAD) urgently requires the establishment of new imaging techniques for early diagnosis and also to guide treatment of patients presented with acute coronary syndromes (ACS). Conventional echocardiography (CE) and electrocardiogram (ECG) are the gold standard methods in assessing myocardial ischemia (MI) and the function of the heart in patients with coronary artery disease (CAD). The lack of ST elevation by ECG and regional wall motion abnormalities by CE in non-ST segment elevation myocardial infarction (NSTEACS) in ACS patients reflect limited sensitivity of ECG and CE in identifying patients with acute coronary occlusion (ACO) and proper assessment of myocardial viability.\ud Aim of this study: This study now evaluates the ability of strain parameters in grading the severity of CAD to detect myocardial viability in ACS through a comparative meta-analysis in two cohorts of patients living in the UAE and Qatar. The study investigates the diagnostic accuracy of left ventricular longitudinal systolic strain function (GLS) by 2D-speckle tracking echocardiography (2D-STE), Territorial Longitudinal Strain (TLS) analysis and post systolic strain (PSS) in ACS patients admitted at the emergency departments. All the patients had acute chest pain which is highly suggestive of NSTEACS along with coronary angiography (CA).\ud Methods: The study recruited two groups, comprising of 347 patients, who were presented with acute coronary syndrome (NSTEACS) at the emergency department. The first group had 214 consecutive patients who had acute chest pain and high-risk profile and they were admitted to the emergency department at Eastern Emirates Hospitals, El-Fujairah-Dibba (EEEH), UAE. The second group consisted of 133 from emergency department at Heart Hospital- Hamad Medical Corporation (HHHMC), Qatar. In both groups, 85% of the patients were men with ages from 32 to 65 years (mean ± SD: 49.4 ± 9.5 years). Significant CAD was defined as having at least one epicardial vessel with ≥ 70% or left main>50% stenosis. All patients enrolled in this study underwent basic echocardiography, speckle tracking analysis, and coronary angiography. In 70 patients, PSS was calculated and myocardial perfusion imaging (MPI) was utilized as gold standards for the assessment of myocardial viability in patients with documented NSTEACS.\ud The sensitivity, specificity, positive and negative predictive values of peak longitudinal systolic strain (2D-STE) and PSS were calculated. Left ventricular systolic strain was displayed as bull’s eye plot and territorial longitudinal strain (TLS) in the territory of the infarct-related artery. They were obtained within 24 hours from admission. Coronary angiography (CA) was performed within 24 hours from admission and used as a reference tests to assess the severity of CAD.\ud Results: Echocardiogram obtained from the patients showed any no wall motion abnormalities at rest, although speckle tracking analysis was abnormal in 167 patients. In the first group of patients from the UAE, GLS showed a high sensitivity of 80% and a very high specificity of 93% for detection of significant CAD. In addition, PSS demonstrated a high sensitivity of 80% with an average specificity of 57%. The combination of GLS and PSS showed a further increase in sensitivity, specificity with positive and negative predictive values of 98%, 91%, 99% and 97%, respectively. Therefore, a very high correlation of GLS and PSS with coronary angiography was demonstrated: =0.90, p

Owing to their superior fluorescence performance, inexpensive synthesis and nontoxic nature, carbon dots (C-dots) are systematically explored in a variety of applications; in this review, we outline and critically discuss recent trends with respect to their potential exploitation in criminal investigation, forensic toxicology and anti-counterfeit interventions. Capitalising on their colour-tuneable behaviour (in the sense that they adopt different colours with respect to the incident radiation), C-dot-based compositions are ideal for the visual enhancement of latent fingerprints, affording improved contrast against multicoloured and patterned backgrounds. As highly sensitive and highly selective optical nanoprobes, C-dots show excellent analytical performance in detecting biological compounds, drugs, explosives, heavy metals and poisonous reactants. In addition, benefiting from their versatile structural and chemical composition, C-dots can be incorporated into ink and polymeric formulations capable of functioning as a new generation of cost-effective barcodes and security nanotags for object authentication and anti-counterfeit applications. Translating these encouraging research outcomes into real-life innovations with significant social and economic impact requires an open, multidisciplinary approach and a close synergy between materials scientists, biologists, forensic investigators and digital engineers.

Studies investigating the differential decomposition of remains on different surfaces have been limited to qualitative data. This study statistically analyses the differences in rate and pattern of decomposition between concrete, gravel and grass surfaces. A total of 11 pigs (Sus scrofa domesticus) were used in the study: three for the concrete and gravel surfaces each, and 5 for the grass surface. Results showed that there was no difference in the overall rate of decomposition between surfaces (p > 0.05); however, there were significant differences in the pattern of decomposition. Mummification was observed on all subjects in the study and may have been the reason for the similarities in the decomposition rates between surfaces. The results of this study show that in the intertwined relationship between factors affecting decomposition surface type may be overshadowed by other environmental factors.

Accumulation of methylglyoxal (MG) arising from downregulation of its primary degrading enzyme glyoxalase-1 (Glo1) is an underlying cause of diabetic cardiomyopathy (DC). This study investigated if expressing Glo1 in rat hearts shortly after the onset of Type 1 diabetes mellitus (T1DM) would blunt the development of DC employing the streptozotocin-induced T1DM rat model, an adeno-associated virus containing Glo1 driven by the endothelin-1 promoter (AAV2/9-Endo-Glo1), echocardiography, video edge, confocal imaging, and biochemical/histopathological assays. After eight weeks of T1DM, rats developed DC characterized by a decreased E:A ratio, fractional shortening, and ejection fraction, and increased isovolumetric relaxation time, E: e&rsquo, ratio, and circumferential and longitudinal strains. Evoked Ca2+ transients and contractile kinetics were also impaired in ventricular myocytes. Hearts from eight weeks T1DM rats had lower Glo1 and GSH levels, elevated carbonyl/oxidative stress, microvascular leakage, inflammation, and fibrosis. A single injection of AAV2/9 Endo-Glo1 (1.7&times, 1012 viron particles/kg) one week after onset of T1DM, potentiated GSH, and blunted MG accumulation, carbonyl/oxidative stress, microvascular leakage, inflammation, fibrosis, and impairments in cardiac and myocyte functions that develop after eight weeks of T1DM. These new data indicate that preventing Glo1 downregulation by administering AAV2/9-Endo-Glo1 to rats one week after the onset of T1DM, blunted the DC that develops after eight weeks of diabetes by attenuating carbonyl/oxidative stresses, microvascular leakage, inflammation, and fibrosis.

COVID-19 is a new viral illness that can affect the lungs and airways with lethal consequences leading to the death of the patients. The ACE2 receptors were widely disturbed among body tissues such as lung, kidney, small intestine, heart, and others in different percent and considered a target for the nCOVID-19 virus. S-protein of the virus was binding to ACE2 receptors caused downregulation of endogenous anti-viral mediators, upregulation of NF-κB pathway, ROS and pro-apoptotic protein. Nrf2 was a transcription factor that's play a role in generation of anti-oxidant enzymes. To describe and establish role of Nrf2 activators for treatment COVID-19 positive patients. We used method of analysis of the published papers with described studies about COVID-19 connected with pharmacological issues and aspects which are included in global fighting against COVID-19 infection, and how using DMF (Nrf2 activator) in clinical trial for nCOVID-19 produce positive effects in patients for reduce lung alveolar cells damage. we are found that Nrf2 activators an important medication that's have a role in reduce viral pathogenesis via inhibit virus entry through induce SPLI gene expression as well as inhibit TRMPSS2, upregulation of ACE2 that's make a competition with the virus on binding site, induce gene expression of anti-viral mediators such as RIG-1 and INFs, induce anti-oxidant enzymes, also they have a role in inhibit NF-κB pathway, inhibit both apoptosis proteins and gene expression of TLRs. We are concluded that use DMF (Nrf2 activator) in clinical trial for nCOVID-19 positive patients to reduce lung alveolar cells damage. [Abstract copyright: © 2020 Saif M Hassan, Mahmood J Jawad, Salam W. Ahjel, Ram B. Singh, Jaipaul Singh4, Samir Mohamed Awad, Najah R Hadi.]

Plastic bag suffocation has been reported in cases of homicide, suicide, and accidental death, with an increase in numbers of suicide and accidental deaths. Though case reports are abundant, decomposition studies have not been performed. This study utilised 20 Sus scrofa domesticus to quantify the effect of a plastic bag covering the head on the rate and pattern of decomposition. A sample group of ten carcasses had plastic bags placed over the heads, with another ten carcasses acting as a control group, without a head covering. The carcasses were placed in an open field to decompose. Over the course of 52 days, data were collected bi-weekly on the rate and pattern of decomposition. The results show that a plastic bag covering the head of a carcass has an overall decreased effect on the rate of decomposition, compared to the control group. The decomposition pattern of head > trunk > limb in decreasing decomposition rate was not affected by the plastic bag; however, in comparison to the control group, the decomposition of the head and trunk regions differed significantly, while the limbs stayed unaffected. The heads of the sample group showed a decrease in decomposition rate, while the trunks showed an increase. This was deemed due to an increase in insect activity at the trunk and a decrease in activity at the head. An altered PMI calculation is provided.

Systemic Hypertension (HTN) accounts for the largest amount of attributable Cardiovascular (CV) mortality worldwide. There are several factors responsible for the development of HTN and its CV complications. Multicenter trials revealed that risk factors responsible for Micro Vascular Disease (MVD) are similar for those attributable to Coronary Artery Disease (CAD) which include tobacco use, unhealthy cholesterol levels, HTN, obesity and overweight, physical inactivity, unhealthy diet, diabetes, insulin resistance, increasing age and genetic predisposition. In addition, the defective release of Nitric Oxide (NO) could be a putative candidate for HTN and MVD. This study reviewed the risk stratification of hypertensive population employing cardiac imaging modalities which are of crucial importance in diagnosis. It further emphasized the proper used of cardiac imaging to determine patients at increased CV risk and identify the management strategy. It is now known that NO has an important eff ect on blood pressure, and the basal release of endothelial Nitric Oxide (eNOS) in HTN may be reduced. Although there are diff erent forms of eNOS gene allele, there is no solid data revealing the potential role of the polymorphism of the eNOS in patients with HTN and coronary vascular diseases. In the present article, the prevalence of eNOS G298 allele in hypertensive patients with micro vascular angina will be demonstrated.\ud This review provides an update on appropriate and justified use of non-invasive imaging tests in hypertensive patients and its\ud important role in proper diagnosis of MVD and CAD. Second, eNOS gene allele and its relation to essential hypertension and angina pectoris are also highlighted.

Aim: This study was designed to assess the utilization of plants with anti-diabetic properties and to conduct preliminary phytochemical analysis and moreover, to assess the anti-diabetic potential of selected plants used to treat and manage Type 2 diabetes mellitus (T2DM) among the residents of Pakuri (St. Cuthbert’s Mission) in Guyana. Methodology: The leaves of sour sop (Annona muricata L.), pear (Persea americana Mill.) and Mocou-Mocou (Montrichardia arborescens L. Schott) underwent phytochemical testing and a-amylase Inhibition testing to determine their anti-diabetic properties. Results: This study showed that the leaves of pear, sour sop and mocou mocou contained different chemical constituents including alkaloids, flavonoids, saponins and tannins but the absence of amino acids (proteins), carbohydrates and glycosides. The a-amylase inhibitory studies performed demonstrated that the extracts of A. muricata in ethanol and chloroform had significant inhibitory potential. Conclusion: Considering the promising potential of phytochemicals and the anti-diabetic activity of these species of plant extracts in anti-diabetic drug development, in vivo experiments and clinical trials are required for efficacy and safety evaluation. Also, the anti-diabetic phytochemicals may be used in combination with existing orthodox drugs, thereby, reducing the dose of synthetic anti-diabetic drugs, which will help in addressing the toxicity and cost-related issues in chronic use during the management of diabetes mellitus (DM).

Linearized esculentin 2 EM (E2EM-lin) from the frog, Glandirana emeljanovi was highly active against Gram-positive bacteria (minimum lethal concentration ≤ 5.0 μM) and strongly α-helical in the presence of lipid mimics of their membranes (>55.0%). The N-terminal α-helical structure adopted by E2EM-lin showed the potential to form a membrane interactive, tilted peptide with an hydrophobicity gradient over residues 9 to 23. E2EM-lin inserted strongly into lipid mimics of membranes from Gram-positive bacteria (maximal surface pressure changes ≥5.5 mN m ), inducing increased rigidity (C ↑), thermodynamic instability (ΔG 0) and high levels of lysis (>50.0%). These effects appeared to be driven by the high anionic lipid content of membranes from Gram-positive bacteria; namely phosphatidylglycerol (PG) and cardiolipin (CL) species. The high levels of α-helicity (60.0%), interaction (maximal surface pressure change = 6.7 mN m ) and lysis (66.0%) shown by E2EM-lin with PG species was a major driver in the ability of the peptide to lyse and kill Gram-positive bacteria. E2EM-lin also showed high levels of α-helicity (62.0%) with CL species but only low levels of interaction (maximal surface pressure change = 2.9 mN m ) and lysis (21.0%) with the lipid. These combined data suggest that E2EM-lin has a specificity for killing Gram-positive bacteria that involves the formation of tilted structure and appears to be primarily driven by PG-mediated membranolysis. These structure/function relationships are used to help explain the pore forming process proposed to describe the membranolytic, antibacterial action of E2EM-lin. [Abstract copyright: Crown Copyright © 2019. Published by Elsevier B.V. All rights reserved.]

espanolLa distribucion del jaguar (Panthera onca) y el puma (Puma concolor) se superponen en la Peninsula de Yucatan, donde se encuentra la poblacion mas importante de jaguares en Mexico. El objetivo de este estudio, realizado en la Reserva Ecologica El Eden, fue estudiar los factores que permiten que estos dos depredadores coexistan en la densa vegetacion de la selva mediana tropical y los bosques secundarios del noreste de la peninsula de Yucatan. En el estudio se evaluo la superposicion en el tiempo y el espacio utilizando el indice de Pianka y se analizo el uso que hacen del habitat estas dos especies mediante modelos de ocupacion. Un esfuerzo de muestreo total de 7.159 noches/trampa durante cuatro anos produjo 142 registros fotograficos independientes de jaguares y 134 de pumas. Los felidos mostraron una superposicion alta o muy alta en el uso de vegetacion (0,68–0,99) y los tipos de senderos (0,63–0,97) y en sus patrones de actividad (0,81–0,90). Sin embargo, sus picos de actividad muestran una cierta separacion temporal. El momento del dia, en particular para los picos de actividad, fue el factor que mejor explicaba la coexistencia de los felidos en este habitat. Los modelos de ocupacion mostraron que la presencia de presas potenciales y el tipo de vegetacion podrian predecir la presencia de felidos en la zona del estudio. Las perturbaciones naturales acaecidas durante 2010 (huracan) y 2011 (incendio) cambiaron drasticamente el uso del habitat y los patrones de actividad de forma que los pumas y los jaguares adaptaron el uso de los recursos y sus patrones de actividad mediante una estrategia de evasion mutua. EnglishThe biological ranges of the jaguar (Panthera onc) and puma (Puma concolor) overlap in the Yucatan Peninsula, corresponding to the most important population of jaguars in Mexico. The goal of this study in the El Eden Ecological Reserve (EER) was to investigate the factors that permit these two predators to coexist in the dense vegetation of medium–stature tropical forest and secondary forest in the north–eastern Yucatan Peninsula. We assessed their spatial and temporal overlap using Pianka’s index, and evaluated their habitat use by applying occupancy models. A total sampling effort of 7,159 trap–nights over 4 years produced 142 independent photographic records of jaguars, and 134 of pumas. The felids showed high to very high overlap in their use of different vegetation (0.68–0.99) and trail types (0.63–0.97) and in their activity patterns (0.81–0.90). However, their peak activity patterns showed some temporal separation. Time of day, particularly for peak activity time, was the best predictor to explain the coexistence of the felids in this habitat. While occupancy models showed that the presence of potential prey species and vegetation type could predict the presence of felids in the study area. Natural disturbances during 2010 (hurricane) and 2011 (fire) drastically changed habitat use and activity patterns, resulting in pumas and jaguars adjusting their resource–use and activity pattern through a strategy of mutual evasion

In order to accelerate the isolation and characterisation of structurally new or novel natural products, it is crucial to develop efficient strategies that prioritise samples with greatest promise early in the workflow so that resources can be utilised in a more efficient and cost-effective manner. Two complementary approaches have been developed: One is based on targeted identification of known compounds held in a database based on high resolution MS and predicted LC retention time data [1]. The second is an MS metrics-based approach where the software algorithm calculates metrics for sample novelty, complexity, and diversity after interrogating databases of known compounds, and contaminants. These metrics are then used to prioritise samples for isolation and structure elucidation work [2]. Both dereplication approaches have been validated using natural product extracts resulting in the isolation and characterization of new or novel natural products.

Mongol-like-horsemen-turned-merchants from Manchuria are known as Manchus, originally their homeland was centered around what is nowadays the city of Shenyang in Northeast China. Previously, worldwide analysis of Y-chromosomal haplotype diversity for 23 STR loci and Y-STR databases with PowerPlex® Y23 System (Promega Corporation Madison, USA) kit were created with collaborative efforts, but Manchu population data was missing. In current study, PowerPlex® Y23 System loci were examined in 328 unrelated Manchu male individuals from Xiuyan and Huanren Manchu autonomous counties in Liaoning province, to calculate the forensic parameters of the 23 STR loci. A total of 323 different haplotypes were observed on these 23 Y-STR loci. The gene diversities ranged from 0.3820 (DYS391) to 0.9696 (DYS385a, b). The overall haplotype diversity was 0.9999 ± 0.0002 at PowerPlex® Y23 System. Rst pairwise analyses, multidimensional scaling plot, and linear discriminatory analysis showed the genetic structure of Manchu population was significantly different from some of Chinese populations like Tibetan and Uyghur. Results of our study showed that PowerPlex® Y23 System marker set provided substantially stronger discriminatory power in Manchu population of China.

In the years since the last volume on plant domesticates in Ireland and Britain in the fourth millennium[AQ1] cal BC (Fairburn, A., ed. [2000]. Plants in Neolithic Britain and Beyond. Oxford: Oxbow)[AQ2], a number of significant changes have occurred in archaeological practice. These have included the routine application of a range of archaeological science techniques, including Bayesian statistical modelling. During the same period, large-scale sampling strategies in developer-led professional practice, and especially on large infrastructure projects, have increased the available materials for study. This paper will present a new analysis of radiocarbon results from direct measurements on domesticated plant remains from Ireland and Britain to explore what these can tell us about the nature of the changes in the archaeological record at this time. Cereals may appear after the introduction of practices including monuments in the form of causewayed enclosures, tombs and post-and-slot-built structures, in several regions. This approach is new because it unpacks the ‘Neolithic package’, by comparing direct estimates on plant domesticates with the appearance of other forms of material culture and new site types. ‘Unpacking the package’ here (or in other periods critically engaging with culture historic-derived analytical constructs) is important in three ways, which will be discussed in this paper. As well as allowing us to examine the fine-grained nature of changes, developments in chronological modelling should cause us to re-examine how useful our existing terms of engagement are; we need to think not only about the changes we can produce in the sequence of archaeological knowledge, but also in the structure of it.

The article presents results of research about Polish services companies. It has been conducted to assess the impact that services liberalization on the EU market has on competitiveness and innovativeness of Polish services companies, which is a result of a EU Services Directive from 2006. The research has been conducted on 400 companies that provide services in such areas: commerce, building, craftsmanship, business specialization, tourism, accommodation services, catering. The idea was to assess how this subject is perceived by the above‑mentioned entities as well as assessment of benefits and barriers that come with the liberalization of services market. Research hypothesis: Services Directive impacts positively the competitiveness and innovativeness of Polish services companies on the EU market and has been positively verified. The article itself is a research. Artykuł przedstawia wyniki badania polskich przedsiębiorstw usługowych przeprowadzonego w celu oceny wpływu liberalizacji usług na unijnym rynku, wprowadzonej dyrektywą usługową z 2006 roku, na konkurencyjność i innowacyjność polskich przedsiębiorstw usługowych. Badanie przeprowadzono wśród 400 przedsiębiorstw świadczących usługi: handlowe, budowlane, rzemieślnicze, specjalistyczne związane z działalnością biznesową, szkoleniowe, edukacyjne i informacyjne, turystyczne, zakwaterowania i wyżywienia. Przedmiotem badania była ocena liberalizacji usług przez podmioty oraz korzyści i barier wynikających z niej dla usługodawców. Na polskim rynku nie ma tak kompleksowego badania przedsiębiorstw usługowych eksportujących swoje usługi na rynki krajów Wspólnoty. Wyniki przedstawione w artykule są efektem oryginalnych, autorskich badań. Sformułowano dwie hipotezy badawcze: (1) dyrektywa usługowa pozytywnie wpływa na konkurencyjność i innowacyjność polskich przedsiębiorstw usługowych na rynku wspólnotowym; (2) przedsiębiorcy w większym stopniu dostrzegają korzyści w obszarze wpływu na konkurencyjność i innowacyjność ich przedsiębiorstw niż bariery implementacji dyrektywy usługowej. Obie hipotezy zostały pozytywnie zweryfikowane. Artykuł ma charakter badawczy.

The challenge for the automotive industry is how to ensure they adopt the circular economy when it comes to the disposal of end-of-life vehicles (ELV). According to the European Commission the UK achieved a total reuse and recovery rate of 88%. This is short of the revised ELV directive target of 95% materials recovery, which requires a minimum of 85% of materials to be recycled or reused. A significant component of the recycling process is the production of automotive shredder residue (ASR). This is currently landfilled across Europe. The additional 10% could be met by processing ASR through either waste-to-energy facilities or Post shredder technology (PST) to recover materials. The UK auto and recycling sectors claimed there would need to be a massive investment by their members in both new capacity and new technology for PST to recover additional recycle materials. It has been shown that 50% of the ASR contains valuable recoverable materials which could be used to meet the Directive target. It is expected in the next 5years that technological innovation in car design will change the composition from easily recoverable metal to difficult polymers. This change in composition will impact on the current drive to integrate the European Circular Economy Package. A positive factor is that main driver for using ASR is coming from the metals recycling industry itself. They are looking to develop the infrastructure for energy generation from ASR and subsequent material recovery. This is driven by the economics of the process rather than meeting the Directive targets. The study undertaken has identified potential pathways and barriers for commercial thermal treatment of ASR. The results of ASR characterisation were used to assess commercial plants from around the world. Whilst there were many claiming that processing of ASR was possible none have so far shown both the technological capability and economic justification. [Abstract copyright: Copyright © 2017 Elsevier Ltd. All rights reserved.]

There is much evidence that diabetes mellitus (DM)-induced hyperglycemia (HG) is responsible for kidney failure or nephropathy leading to cardiovascular complications. Cellular and molecular mechanism(s) whereby DM can damage the kidney is still not fully understood. This study investigated the effect of streptozotocin (STZ)-induced diabetes (T1DM) on the structure and associated molecular alterations of the isolated rat left kidney following 2 and 4 months of the disorder compared to the respective age-matched controls. The results revealed hypertrophy and general disorganized architecture of the kidney characterized by expansion in glomerular borders, tubular atrophy and increased vacuolization of renal tubular epithelial cells in the diabetic groups compared to controls. Electron microscopic analysis revealed ultrastructural alterations in the left kidney highlighted by an increase in glomerular basement membrane width. In addition, increased caspase-3 immunoreactivity was observed in the kidney of T1DM animals compared to age-matched controls. These structural changes were associated with elevated extracellular matrix (ECM) deposition and consequently, altered gene expression profile of ECM key components, together with elevated levels of key mediators (MMP9, integrin 5α, TIMP4, CTGF, vimentin) and reduced expressions of Cx43 and MMP2 of the ECM. Marked hypertrophy of the kidney was highlighted by increased atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) gene expression. These changes also correlated with increased TGFβ1 activity, gene expression in the left kidney and elevated active TGFβ1 in the plasma of T1DM rats compared to control. The results clearly demonstrated that TIDM could elicit severe structural changes and alteration in biochemical markers (remodelling) in the kidney leading to diabetic nephropathy (DN).

For the last three decades, Short Tanddem Repeat (STR) markers and capillary electrophoresis-based DNA sequencers have been the gold standard technology for human identification testing in the forensic field. Massive Parallel Sequencing (MPS) technology has enabled sequencing of several types of genetic loci in one multiplex including single nucleotide polymorphisms (SNPs) and STRs. One hundred and fifty (150) reference samples were profiled using the ForenSeq™ DNA Signature kit. PCR Primer Mix B for this kit was used containing autosomal/Y/X STRs and AISNPS, iSNPs and pSNPs loci. The analyses of the sequence of STR alleles in STRait Razor software were able to determine novel alleles in the autosomal, Y and X STR as well as SNPs loci. This increased number of alleles had a clear advantage in forensic casework. Several of these sequenced-based alleles had not been reported earlier in the literature. This study also confirmed some of the previously determined alleles in several loci. The results have clearly demonstrated the potential use of MPS methods to study the genetics of Qatari population.

Differentiating male lineages using non-recombining Y-chromosomal genetic markers is highly informative for tracing human migration and for forensic studies. The aim of this study was to develop allelic frequency to evaluate the resolution power of Rapidly Mutating Y-STR (RMY-STR) on Qatar population in comparison to Worldwide population. In Qatari population the haplotype resolution of 100 % was achieved. The set of 13 RMY-STR markers showed remarkable haplotype resolution power in the Qatar population, high gene diversity and sufficient robustness. The novel multiplex PCR reaction wasused to type 250 samples from unrelated Qatari males. A total of 250 different haplotypes were det, with no shared haplotypes between individuals. The overall haplotype diversity (HD) was 100 %. It was found that the markers which contributed the most toward high HD were DYF399S1 and DYF403S1a/b. FST values were generated and AMOVA test performed between Qatar population and Middle Eastern/several worldwide population data sets were undertaken for FST values; geography was found to account considerably for the pattern of population sub structuring, although some discrepancies were observed in the relationship between geographical and genetic distance.

Whilst studies refer to the community impact of Organized Crime (OC), no survey currently exists to examine the views of those citizens who reside in areas where Organized Crime Groups (OCGs) proliferate. 431 questionnaires from households co-existing in high density OCGs areas were analysed in relation to: a) demographic information; b) views on the community and the police; and c) how they expected other residents to react to illegal incidents. Overall respondents thought the average citizen would refuse to intervene in 10% - 48% of illegal incidents, with the specific case influencing whether and how they would respond. The analysis then compared three communities who lived in high density OCG areas with a control community (n = 343). The ‘OCG’ communities were more likely to report low collective efficacy and were generally least likely to expect their neighbours to confront a crime in action. Conversely, whilst the control group showed higher levels of collective efficacy and expected the average resident to be more likely in confronting illegal behaviour, this trend did not extend to street drug dealing and serious crime associated with OC. The study discusses the unreported intimidation associated with OCGs and the challenges of policing hostile environments.

Escherichia coli has developed sophisticated means to sense, respond, and adapt in stressed environment. It has served as a model organism for studies in molecular genetics and physiology since the 1960s. Stress response genes are induced whenever a cell needs to adapt and survive under unfavorable growth conditions. Two of the possible important genes are rpoS and bolA. The rpoS gene has been known as the alternative sigma () factor, which controls the expression of a large number of genes, which are involved in responses to various stress factors as well as transition to stationary phase from exponential form of growth. Morphogene bolA response to stressed environment leads to round morphology of E. coli cells, but little is known about its involvement in biofilms and its development or maintenance. This study has been undertaken to address the adherence pattern and formation of biofilms by E. coli on stainless steel, polypropylene, and silicone surfaces after 24 h of growth at 37 °C. Scanning electron microscopy was used for direct examination of the cell attachment and biofilm formation on various surfaces and it was found that, in the presence of bolA, E. coli cells were able to attach to the stainless steel and silicone very well. By contrast, polypropylene surface was not found to be attractive for E. coli cells. This indicates that bolA responded and can play a major role in the presence and absence of rpoS in cell attachment., info:eu-repo/semantics/publishedVersion

Blowfly species of the family Calliphoridae can be used in forensic investigations to estimate the minimum post-mortem interval (PMI¬min). Lucilia caesar and Lucilia illustris (Diptera: Calliphoridae) are closely related and phenotypically similar, making reliable identification difficult, especially if specimens are in poor condition. To identify potential markers to genetically distinguish these species five complete mitochondrial genomes were sequenced: three for L. caesar (KM657111- KM657113) and two mitochondrial genomes for L. illustris (KM657109, KM 657110). The ND6 gene contained the most species-specific SNPs (1.71%), followed by the ND5 gene (1.68%) and then the COI gene (1.56%), identifying ND6 and ND5 as valuable loci for differentiating L. Caesar and L. illustris specimens.

Tricyclic carbazole is an important scaffold in many naturally occurring metabolites, as well as valuable building blocks. Here we report the reconstitution of the ring A formation of the bacterial neocarazostatin A carbazole metabolite. We provide evidence of the involvement of two unusual aromatic polyketide proteins. This finding suggests how new enzymatic activities can be recruited to specific pathways to expand biosynthetic capacities. Finally, we leveraged our bioinformatics survey to identify the untapped capacity of carbazole biosynthesis.

Hypertensive Cardiomyopathy (HTN-CM) is a structural cardiac disorder generally accompanied by concentric or eccentric Left Ventricular Hypertrophy (LVH) associated with diastolic or/and systolic dysfunction in patients with persistent systemic hypertension. It occurs in the absence of other cardiac diseases capable of causing myocardial hypertrophy or cardiac dysfunction. Long standing arterial hypertension (HTN) leads to structural and functional myocardial abnormalities resulting in myocardial ischemia, fibrosis, and hypertrophy. HTN-CM is predominantly a disease of impaired relaxation rather than impaired contractility, although subtle myocardial systolic abnormalities could be detected recently by Global Longitudinal Systolic Strain (GLS) Speckle Tracking Echocardiography (STE). Importantly, the accompanying LVH is itself a risk factor for mortality and morbidity and is considered an independent predictor for Sudden Cardiac Death (SCD). Therefore, early detection of LVH development in patients with Hypertensive Hypertrophic Cardiomyopathy (HTN-CM) is crucial for optimal treatment. In addition to pathological findings, echocardiography and cardiac magnetic resonance imaging are ideal tools for the diagnosis of HTN-CM and can differentiate it from Hypertrophic Cardiomyopathy (HCM). Timely diagnosis of this condition and utilization of appropriate treatment are required to improve morbidity and mortality in hypertensive patients. This review presents an overview of utilization of multidisciplinary imaging modalities approach for proper diagnosis of HTN-CM and its differentiation from HCM. Relevant article highlighted key points in differentiation of HTN-CM from HCM and the effects of hypertension on cardiac hypertrophy and heart failure development are discussed in clinical case study.

For the last three decades, Short Tandem Repeat (STR) markers and capillary electrophoresis-based DNA sequencers have been the gold standard technology for human identification testing in the forensic field. All laboratories globally, including the Forensic Laboratory, have strategies in place for streamlined processing of high numbers of reference and casework samples, using instrumentation and technologies to maximize output using capillary electrophoresis (CE) based technology so far. Massoive Parallel Sequencing (MPS) technology has enabled sequencing of several types of genetic loci in one multiplex including single nucleotide polymorphisms (SNPs) and STRs. including the STRs using commonly in CE based flourescent multiplex kits. Therefore MPS has the potential to replace CE based technology. One hundred and fifty (150) reference samples from Qatari population were profiled using the ForenSeq™ DNA Signature kit. The data generated through MPS were analysed using ForenSeq™ Universal software and STRait Razor V3 for the primary, secondary and tertiary analyses. The average read depth was 20,000 reads for all sequencing runs. The analyses of the sequence of alleles in STRait Razor software were able to determine novel alleles in the Identntiy SNPs loci. The Qatari population has been a melting pot of various populations and this forensic study was the first of its kind to generate new data on the genetics of Qatari population. The 95 identity SNPs allele frequency data for 150 samples were analysed. The results have clearly demonstrated the potential use of MPS methods to study the genetics of Qatari population.

SNPs are good predictors of ethnicity and serval panels have been published [1]. The ForenSeq Signature kit (Illumina) offers coverage of 230 different markers including 55 ancestry SNPs (AISNPs). The ForenSeq Universal Analysis Software (UAS) brings the capability to analyse the sequencing data, represent results and accomplish statistical estimates of biogeographic ancestry. The ancestry prediction results in UAS are based on Principal Component Analysis (PCA) collected on several reference populations comprised in the 1000 Genomes project. This set does not include Qatari population. The data was analysed using STRUCTURE software. These data serves as an addition to the existing Middle Eastern population data for the 55 AISNPs. The Qatari population has been a melting pot of various populations and this forensic study was the first of its kind to generate new data on the genetics of Qatari population. The 55-ancestry marker allele frequency data for 150 samples were analysed and compared to 139 world populations using STRUCTURE software. FROG-kb analysis led to all Qatari samples being correctly assigned to Middle Eastern populations showing that the Illumina software needs to be enhanced by adding more databases from the Middle East. In conclusion, the results have clearly demonstrated the potential use of MPS methods to study the genetics of Qatari population.

Touch DNA profiling is an important tool to solve the mystery of many cases, especially when other biological evidences cannot be found in crime scene. However, there are many variables that influence Touch DNA profiling such as recovery techniques and extraction. In addition, effect of environmental factors on items found outdoor such as sand can impact on the process. Therefore the aim of this experiment was to test how sandy surfaces can affect the recovery of Touch DNA Profiling by validation two recovery methods and two extraction kits that are widely used in the DNA forensic field.