A study of genetic analysis using novel rapidly mutating Y-STR multiplex for Qatari population

Differentiating male lineages using non-recombining Y-chromosomal genetic markers is highly informative for tracing human migration and for forensic studies. The aim of this study was to develop allelic frequency to evaluate the resolution power of Rapidly Mutating Y-STR (RMY-STR) on Qatar population in comparison to Worldwide population. In Qatari population the haplotype resolution of 100 % was achieved. The set of 13 RMY-STR markers showed remarkable haplotype resolution power in the Qatar population, high gene diversity and sufficient robustness. The novel multiplex PCR reaction wasused to type 250 samples from unrelated Qatari males. A total of 250 different haplotypes were det, with no shared haplotypes between individuals. The overall haplotype diversity (HD) was 100 %. It was found that the markers which contributed the most toward high HD were DYF399S1 and DYF403S1a/b. FST values were generated and AMOVA test performed between Qatar population and Middle Eastern/several worldwide population data sets were undertaken for FST values; geography was found to account considerably for the pattern of population sub structuring, although some discrepancies were observed in the relationship between geographical and genetic distance.