27 results on '"Coluccio, M. L."'
Search Results
2. Silica diatom shells tailored with Au nanoparticles enable sensitive analysis of molecules for biological, safety and environment applications
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Onesto, V., Villani, M., Coluccio, M. L., Majewska, R., Alabastri, A., Battista, E., Schirato, A., Calestani, D., Coppedé, N., Cesarelli, M., Amato, F., Di Fabrizio, E., and Gentile, F.
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- 2018
- Full Text
- View/download PDF
3. Relating the rate of growth of metal nanoparticles to cluster size distribution in electroless deposition
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Iatalese, M., primary, Coluccio, M. L., additional, Onesto, V., additional, Amato, F., additional, Di Fabrizio, E., additional, and Gentile, F., additional
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- 2019
- Full Text
- View/download PDF
4. Nano-topography Enhances Communication in Neural Cells Networks
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Onesto, V., primary, Cancedda, L., additional, Coluccio, M. L., additional, Nanni, M., additional, Pesce, M., additional, Malara, N., additional, Cesarelli, M., additional, Di Fabrizio, E., additional, Amato, F., additional, and Gentile, F., additional
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- 2017
- Full Text
- View/download PDF
5. Release of proteinic and non-proteinic compounds from novel ionisable hydrogels: effect of pH on swelling and drug delivery behaviour
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Barbani, N, Tricoli, F, Bertoni, F, Coluccio, M. L., Ciardelli, Gianluca, Silvestri, D, and Cristallini, C.
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- 2006
6. Poly(ethylene-co-vinyl alcohol) membranes with specific adsorption properties for potential clinical applications
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Silvestri, D, Coluccio, M. L., Barbani, N, Ciardelli, Gianluca, Cristallini, C, Pegoraro, C, and Giusti, P.
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- 2006
7. Preparation of Temporary Structures based onGellan/Adipic Acid Materials for Tissue Reconstruction
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Coluccio, M. L., Guerra, G. D., Cristallini, C., Ciardelli, Gianluca, Bertoni, F., Silvestri, D., Giusti, P., and Barbani, N.
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- 2005
8. Poly(Vinyl Alcohol-co-Acrylic acid) Copolymer As Smart Drug delivery System
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Barbani, N., Ciardelli, G., Cristallini, C., Bertoni, F., Coluccio, M. L., Ferri, F., Tricoli, F., and Giusti, Paolo
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- 2004
9. Preparation and characterization of poly(Vinyl Alcohol-co-Acrylic Acid) microparticles as a smart drug delivery system
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Coluccio, M. L., Barbani, N., Bertoni, F., Ciardelli, G., Cristallini, C., and Giusti, Paolo
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- 2004
10. Gellan/hydroxyapatite composites for preparation of temporary structures for bone reconstruction
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Coluccio, M. L., Barbani, N, Guerra, G. D., Ciardelli, Gianluca, Ferri, F, Krajewski, A, Mazzocchi, M, and Ravaglioli, A.
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TGA ,Spotlight FT-IR ,Gellan ,SEM ,Hydroxyapatite - Abstract
Gellan solution in hot water (1% w/v) was mixed with solid hydroxyapatite under stirring, and the hot suspensions rapidly quenched in liquid nitrogen; the gels so obtained were lyophilised. The material, tested by SEM, appears quite porous. FT-IR spectra show that the carboxylate band at about 1600 cm-1 is slightly altered, likely due to some interaction with the ionic hydroxyapatite structure. Thermogravimetric analysis of the composites shows a slight shift towards higher temperatures of the main signal of the gellan decomposition at 258°C; that shift increases with increasing hydroxyapatite to gellan ratio. Such behaviour may be indicative of a slight thermal stabilisation of gellan, due to interaction with hydroxyapatite.
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- 2004
11. Produzione e caratterizzazione di membrane a porosità controllata mediante attacco enzimatico
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Barbani, N, Coluccio, M. L., Ciardelli, G, Cristallini, C, Lazzeri, Luigi, and Giusti, Paolo
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- 2003
12. NEW BIOMATERIALS BASED ON GELLAN GUM AND GELATIN
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Barbani, N, Bertoni, F, Ciardelli, Gianluca, Cristallini, C, Cascone, M. G., Coluccio, M. L., and Giusti, P.
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- 2003
13. Production and characterization of enzymatic controlled porosity membranes
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Barbani, N., Coluccio, M. L., Ciardelli, G., Cristallini, C., Lazzeri, Luigi, Bertoni, F., and Giusti, Paolo
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- 2003
14. Surface modification of poly(Ethylene-co-vinyl alcohol) membranes by molecular imprinting technique for biomedical application
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Coluccio, M. L., Pegoraro, C., Silvestri, D., Cristallini, C., Gianluca Ciardelli, and Barbani, N.
15. Transforming diatomaceous earth into sensing devices by surface modification with gold nanoparticles
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Valentina Onesto, Immanuel Valpapuram, Edmondo Battista, A. Zappettini, Marco Villani, Andrea Schirato, Maria Laura Coluccio, Roksana Majewska, Francesco Gentile, Nicola Coppedè, E. Di Fabrizio, Alessandro Alabastri, Davide Calestani, Francesco Amato, Villani, M., Onesto, V., Coluccio, M. L., Valpapuram, I., Majewska, R., Alabastri, A., Battista, E., Schirato, A., Calestani, D., Coppedé, N., Zappettini, A., Amato, F., Di Fabrizio, E., Gentile, F., and 29675146 - Majewska, Roksana
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Diatomite ,Materials science ,Gold nanoparticle ,Nanoparticle ,lcsh:TK7800-8360 ,symbols.namesake ,lcsh:Technology (General) ,Gold nanoparticles ,Photo-deposition ,Electrical and Electronic Engineering ,Porosity ,Deposition (law) ,Diatoms ,biology ,SERS ,fungi ,lcsh:Electronics ,Diatom ,Condensed Matter Physics ,biology.organism_classification ,Atomic and Molecular Physics, and Optics ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Chemical engineering ,Colloidal gold ,symbols ,Surface modification ,lcsh:T1-995 ,Raman spectroscopy ,Biosensor ,Bio-sensors - Abstract
Diatomaceous earth, or diatomite, is produced through the accumulation of diatom (Bacillariophyceae) skeletons (i.e. cell walls called frustules) made of amorphous silica. The porous, highly symmetrical structure and microscopic size of diatom cell walls make them ideal constituents of sensing devices and analytical chips. Here, we propose chemical methods to purify diatom frustules extracted from diatomaceous earth. Using photo deposition techniques, we grow gold nanoparticles on the surface of diatom skeletons and within the pores of the skeletons, where the size and density of nanoparticles can be controlled by changing the parameters of the synthesis. Resulting devices have an internal porous structure that can harvest molecules from a solution, and an external shell of gold nanoparticles that amplifies the electromagnetic field generated by the measurement laser in Raman or other spectroscopies. The combination of these effects enables the analysis of biological specimens, chemical analytes and pollutants in extremely low abundance ranges. The devices were demonstrated in the analysis of Bovine Serum Albumin in water with a concentration of 100 aM, and mineral oil with a concentration of 50 ppm. Keywords: Diatomite, Diatoms, Photo-deposition, Gold nanoparticles, SERS, Bio-sensors
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- 2019
16. Relating the rate of growth of metal nanoparticles to cluster size distribution in electroless deposition
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Francesco Gentile, M. Iatalese, Francesco Amato, Maria Laura Coluccio, E. Di Fabrizio, Valentina Onesto, Iatalese, M., Coluccio, M. L., Onesto, V., Amato, F., Di Fabrizio, E., and Gentile, F.
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Materials science ,Silicon ,Metal ions in aqueous solution ,Diffusion ,General Engineering ,Analytical chemistry ,chemistry.chemical_element ,Nanoparticle ,Bioengineering ,General Chemistry ,Substrate (electronics) ,Atomic and Molecular Physics, and Optics ,Nanomaterials ,Metal ,chemistry ,visual_art ,Diffusion-limited aggregation ,visual_art.visual_art_medium ,General Materials Science - Abstract
Electroless deposition on patterned silicon substrates enables the formation of metal nanomaterials with tight control over their size and shape. In the technique, metal ions are transported by diffusion from a solution to the active sites of an autocatalytic substrate where they are reduced as metals upon contact. Here, using diffusion limited aggregation models and numerical simulations, we derived relationships that correlate the cluster size distribution to the total mass of deposited particles. We found that the ratio ξ between the rates of growth of two different metals depends on the ratio γ between the rates of growth of clusters formed by those metals through the linearity law ξ = 14(γ − 1). We then validated the model using experiments. Different from other methods, the model derives k using as input the geometry of metal nanoparticle clusters, decoded by SEM or AFM images of samples, and a known reference.
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- 2019
17. A passive microfluidic device for chemotaxis studies
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Maria Antonia D'Attimo, Ennio Carbone, Costanza Maria Cristiani, Enzo Di Fabrizio, Gerardo Perozziello, Ulrich Krühne, Giovanni Cuda, Francesco Guzzi, Elvira Immacolata Parrotta, Patrizio Candeloro, Elisabetta Dattola, Maria Laura Coluccio, E. Lamanna, Coluccio, M. L., D'Attimo, M. A., Cristiani, C. M., Candeloro, P., Parrotta, E., Dattola, E., Guzzi, F., Cuda, G., Lamanna, E., Carbone, E., Kruhne, U., Di Fabrizio, E., and Perozziello, G.
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Materials science ,Microscope ,Gravity force ,Diffusion ,lcsh:Mechanical engineering and machinery ,Microfluidics ,02 engineering and technology ,Mini incubator ,01 natural sciences ,Passive microfluidic device ,Article ,law.invention ,law ,lcsh:TJ1-1570 ,Electrical and Electronic Engineering ,chemotaxis ,business.industry ,Mechanical Engineering ,Chemotaxis ,010401 analytical chemistry ,Incubator ,Chemotaxi ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Volumetric flow rate ,Control and Systems Engineering ,Optoelectronics ,0210 nano-technology ,business ,Concentration gradient - Abstract
This work presents a disposable passive microfluidic system, allowing chemotaxis studies, through the generation of a concentration gradient. The device can handle liquid flows without an external supply of pressure or electric gradients, but simply using gravity force. It is able to ensure flow rates of 10 µ, L/h decreasing linearly with 2.5% in 24 h. The device is made of poly(methylmethacrylate) (PMMA), a biocompatible material, and it is fabricated by micro-milling and solvent assisted bonding. It is assembled into a mini incubator, designed properly for cell biology studies in passive microfluidic devices, which provides control of temperature and humidity levels, a contamination-free environment for cells with air and 5% of CO2. Furthermore, the mini incubator can be mounted on standard inverted optical microscopes. By using our microfluidic device integrated into the mini incubator, we are able to evaluate and follow in real-time the migration of any cell line to a chemotactic agent. The device is validated by showing cell migration at a rate of 0.36 µ, m/min, comparable with the rates present in scientific literature.
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- 2019
18. Combined effect of surface nano-topography and delivery of therapeutics on the adhesion of tumor cells on porous silicon substrates
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Natalia Malara, S. A. De Pascali, Francesco Gentile, Gerardo Perozziello, S. De Vitis, Maria Laura Coluccio, G. Strumbo, P. Candeloro, Francesco Paolo Fanizzi, E. Di Fabrizio, DE VITIS, Salvatore, Coluccio, M. L., Strumbo, G., Malara, N., Fanizzi, Francesco Paolo, DE PASCALI, SANDRA ANGELICA, Perozziello, G., Candeloro, P., Di Fabrizio, E., Gentile, Fabrizio, De Vitis, S., Fanizzi, F. P., De Pascali, S. A., and Gentile, Francesco
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0301 basic medicine ,Atomic and Molecular Physics, and Optic ,Materials science ,Biocompatibility ,Silicon ,Nano-topography ,Surfaces, Coatings and Film ,chemistry.chemical_element ,Nanotechnology ,Condensed Matter Physic ,02 engineering and technology ,Porous silicon ,Nanomaterials ,03 medical and health sciences ,Tissue engineering ,Electrical and Electronic Engineering ,Cell adhesion ,Electronic, Optical and Magnetic Material ,technology, industry, and agriculture ,Adhesion ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Anti-tumor drug ,Atomic and Molecular Physics, and Optics ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,030104 developmental biology ,chemistry ,Drug delivery ,0210 nano-technology - Abstract
Porous silicon is a nano material in which pores with different sizes, densities and depths are infiltrated in conventional silicon imparting it augmented properties including biodegradability, biocompatibility, photoluminescence. Here, we realized porous silicon substrates in which the pore size and the fractal dimension were varied over a significant range. We loaded the described substrates with a PtCl(O,O'-acac)(DMSO) antitumor drug and determined its release profile as a function of pore size over time up to 15days. We observed that the efficacy of delivery augments with the pore size moving from small (~8nm, efficiency of delivery ~0.2) to large (~55nm, efficiency of delivery ~0.7). Then, we verified the adhesion of MCF-7 breast cancer cells on the described substrates with and without the administration of the antitumor drug. This permitted to decouple and understand the coincidental effects of nano-topography and a controlled dosage of drugs on cell adhesion and growth. While large pore sizes guarantee elevated drug dosages, large fractal dimensions boost cell adhesion on a surface. For the particular case of tumor cells and the delivery of an anti-tumor drug, substrates with a small fractal dimension and large pore size hamper cell growth. The competition between nano-topography and a controlled dosage of drugs may either accelerate or block the adhesion of cells on a nanostructured surface, for applications in tissue engineering, regenerative medicine, personalized lab-on-a-chips, and the rational design of implantable drug delivery systems. Display Omitted We realized porous silicon substrates with a varying pore size and fractal dimension.We loaded the substrates with an antitumor drug and determined its release profile over time.We verified the adhesion of MCF-7 cancer cells on the porous substrates.We decoupled the effects of nano-topography and drug delivery on cell adhesion.Large pore sizes boost drug release, large fractal dimensions accelerate cell adhesion.
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- 2016
19. Superhydrophobic lab-on-chip measures secretome protonation state and provides a personalized risk assessment of sporadic tumour
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S. Bonacci, Ivan Presta, Rosario Sacco, Elisabetta Ferraro, Nicola Coppedè, M. Greco, Natalia Malara, Ugo Bottoni, Domenica Scumaci, Giuseppe Donato, Gianni Cuda, Roksana Majewska, Volpentesta G, Francesco Gentile, A. Donato, Valentina Trunzo, Giusy Guzzi, Nadia Innaro, Domenico Augusto Francesco Maisano, Valentina Onesto, A. Castellini, C. K. Pirrone, P. Candeloro, Chiara Mignogna, Gerardo Perozziello, Francesco Amato, F. Casale, Maria Laura Coluccio, F. Givigliano, Lorenzo Ferrara, C. Voci, M. Renne, E. Di Fabrizio, Vincenzo Mollace, Marco Giannetto, Giuseppe Sena, Angelo Lavano, Elisabetta Scali, Maria Careri, Malara, N., Gentile, F., Coppedè, N., Coluccio, M. L., Candeloro, P., Perozziello, G., Ferrara, L., Giannetto, M., Careri, M., Castellini, A., Mignogna, C., Presta, I., Pirrone, C. K., Maisano, D., Donato, A., Donato, G., Greco, M., Scumaci, D., Cuda, G., Casale, F., Ferraro, E., Bonacci, S., Trunzo, V., Mollace, V., Onesto, V., Majewska, R., Amato, F., Renne, M., Innaro, N., Sena, G., Sacco, R., Givigliano, F., Voci, C., Volpentesta, G., Guzzi, G., Lavano, A., Scali, E., Bottoni, U., and Di Fabrizio, E.
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0301 basic medicine ,Oncology ,Cancer Research ,medicine.medical_specialty ,tumor early detection ,lab-on-a-chip ,business.industry ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Cancer Early Detection ,lcsh:RC254-282 ,Article ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Text mining ,030220 oncology & carcinogenesis ,Internal medicine ,oect ,medicine ,False positive paradox ,Cancer risk ,business ,Risk assessment ,Sporadic cancer - Abstract
Secretome of primary cultures is an accessible source of biological markers compared to more complex and less decipherable mixtures such as serum or plasma. The protonation state (PS) of secretome reflects the metabolism of cells and can be used for cancer early detection. Here, we demonstrate a superhydrophobic organic electrochemical device that measures PS in a drop of secretome derived from liquid biopsies. Using data from the sensor and principal component analysis (PCA), we developed algorithms able to efficiently discriminate tumour patients from non-tumour patients. We then validated the results using mass spectrometry and biochemical analysis of samples. For the 36 patients across three independent cohorts, the method identified tumour patients with high sensitivity and identification as high as 100% (no false positives) with declared subjects at-risk, for sporadic cancer onset, by intermediate values of PS. This assay could impact on cancer risk management, individual’s diagnosis and/or help clarify risk in healthy populations., Diagnostics: Proton state of secreted proteins in blood helps identify cancer A blood test that measures whether molecules secreted by cells contain titratable proton atoms can accurately discriminate between patients who have cancer and those who don’t. Titratable species may in turn influence the protonation state of a solution, i.e. the number of protons added to and the net charge of that solution. A team led by Natalia Malara from University Magna Graecia in Catanzaro, Italy and Enzo Di Fabrizio from the King Abdullah University of Science and Technology in Thuwal, Saudi Arabia, Francesco Gentile from the University Federico II in Naples, Italy, and Nicola Coppedè from the Institute of Materials for Electronics and Magnetism in Parma, Italy, created an eletrochemical device that can detect faulty metabolism by quantifying the proportion of secreted proteins with and without extra protons—an indicator of abnormal cell division, proliferation and invasion. The researchers tested the device on blood samples from patients with solid tumors and healthy controls. The method identified cancer patients with a high degree of accuracy. If the findings are confirmed in larger trials, the test could help with the screening, diagnosis and management of cancer.
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- 2018
20. Silica diatom shells tailored with Au nanoparticles enable sensitive analysis of molecules for biological, safety and environment applications
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Roksana Majewska, Nicola Coppedè, Francesco Gentile, Davide Calestani, Francesco Amato, Andrea Schirato, Marco Villani, Edmondo Battista, Maria Laura Coluccio, Valentina Onesto, E. Di Fabrizio, Mario Cesarelli, Alessandro Alabastri, Onesto, V., Villani, M., Coluccio, M. L., Majewska, R., Alabastri, A., Battista, E., Schirato, A., Calestani, D., Coppedé, N., Cesarelli, M., Amato, F., Di Fabrizio, E., and Gentile, F.
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Au functionalization ,Materials science ,Sensing device ,Silicon dioxide ,Nanochemistry ,Nanoparticle ,Nanotechnology ,02 engineering and technology ,01 natural sciences ,Biological sensing ,chemistry.chemical_compound ,0103 physical sciences ,lcsh:TA401-492 ,medicine ,Molecule ,Gold Nanoparticles ,General Materials Science ,Mineral oil ,010302 applied physics ,Diatoms ,biology ,Nano Express ,SERS ,fungi ,Diatom ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,biology.organism_classification ,Sensing devices ,chemistry ,Colloidal gold ,Frustule ,lcsh:Materials of engineering and construction. Mechanics of materials ,Frustules ,Materials Science (all) ,Safety ,0210 nano-technology ,Drug carrier ,medicine.drug - Abstract
Diatom shells are a natural, theoretically unlimited material composed of silicon dioxide, with regular patterns of pores penetrating through their surface. For their characteristics, diatom shells show promise to be used as low cost, highly efficient drug carriers, sensor devices or other micro-devices. Here, we demonstrate diatom shells functionalized with gold nanoparticles for the harvesting and detection of biological analytes (bovine serum albumin—BSA) and chemical pollutants (mineral oil) in low abundance ranges, for applications in bioengineering, medicine, safety, and pollution monitoring. Electronic supplementary material The online version of this article (10.1186/s11671-018-2507-4) contains supplementary material, which is available to authorized users.
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- 2018
21. Nano-topography Enhances Communication in Neural Cells Networks
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Valentina Onesto, Francesco Amato, Mario Cesarelli, Francesco Gentile, Natalia Malara, Mattia Pesce, Laura Cancedda, E. Di Fabrizio, Maria Laura Coluccio, M. Nanni, Onesto, V, Cancedda, L, Coluccio, M. L, Nanni, M, Pesce, M, Malara, N, Cesarelli, Mario, Di Fabrizio, E, Amato, F, and Gentile, Francesco
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0301 basic medicine ,Cell signaling ,Computer science ,lcsh:Medicine ,02 engineering and technology ,Cell Communication ,Microscopy, Atomic Force ,Regenerative medicine ,Article ,03 medical and health sciences ,Mice ,Calcium imaging ,Tissue engineering ,Pregnancy ,Animals ,lcsh:Science ,Topology (chemistry) ,Cells, Cultured ,Neurons ,Multidisciplinary ,Artificial neural network ,Regeneration (biology) ,lcsh:R ,021001 nanoscience & nanotechnology ,Molecular Imaging ,030104 developmental biology ,Microscopy, Fluorescence ,lcsh:Q ,Calcium ,Female ,Neural Networks, Computer ,Nerve Net ,0210 nano-technology ,Biological system ,Energy (signal processing) - Abstract
Neural cells are the smallest building blocks of the central and peripheral nervous systems. Information in neural networks and cell-substrate interactions have been heretofore studied separately. Understanding whether surface nano-topography can direct nerve cells assembly into computational efficient networks may provide new tools and criteria for tissue engineering and regenerative medicine. In this work, we used information theory approaches and functional multi calcium imaging (fMCI) techniques to examine how information flows in neural networks cultured on surfaces with controlled topography. We found that substrate roughness S a affects networks topology. In the low nano-meter range, S a = 0–30 nm, information increases with S a . Moreover, we found that energy density of a network of cells correlates to the topology of that network. This reinforces the view that information, energy and surface nano-topography are tightly inter-connected and should not be neglected when studying cell-cell interaction in neural tissue repair and regeneration.
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- 2017
22. Microfluidics & nanotechnology: towards fully integrated analytical devices for the detection of cancer biomarkers
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Giovanni Cuda, E. Di Fabrizio, Patrizio Candeloro, Francesca Pardeo, Rossella Catalano, Horacio D. Espinosa, Andrea Adamo, Maria Laura Coluccio, Annalisa Nicastri, Elvira Immacolata Parrotta, Angela Mena Perri, Francesco Gentile, Gerardo Perozziello, Perozziello, G., Candeloro, P., Gentile, Francesco, Nicastri, A., Perri, A., Coluccio, M. L., Adamo, A., Pardeo, F., Catalano, R., Parrotta, E., Espinosa, H. D., Cuda, G., and Di Fabrizio, E.
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Materials science ,Resolution (mass spectrometry) ,General Chemical Engineering ,Chemistry (all) ,Microfluidics ,Nanotechnology ,General Chemistry ,symbols.namesake ,Small peptide ,symbols ,Coming out ,Chemical Engineering (all) ,Raman spectroscopy ,Biosensor ,Hydrodynamic flow ,Raman scattering - Abstract
In this paper, we describe an innovative modular microfluidic platform allowing filtering, concentration and analysis of peptides from a complex mixture. The platform is composed of a microfluidic filtering device and a superhydrophobic surface integrating surface enhanced Raman scattering (SERS) sensors. The microfluidic device was used to filter specific peptides (MW 1553.73 D) derived from the BRCA1 protein, a tumor-suppressor molecule which plays a pivotal role in the development of breast cancers, from albumin (66.5 KD), the most represented protein in human plasma. The filtering process consisted of driving the complex mixture through a porous membrane having a cut-off of 12–14 kD by hydrodynamic flow. The filtered samples coming out of the microfluidic device were subsequently deposited on a superhydrophobic surface formed by micro pillars on top of which nanograins were fabricated. The nanograins coupled to a Raman spectroscopy instrument acted as a SERS sensor and allowed analysis of the filtered sample on top of the surface once it evaporated. By using the presented platform, we demonstrate being able to sort small peptides from bigger proteins and to detect them by using a label-free technique at a resolution down to 0.1 ng μL−1. The combination of microfluidics and nanotechnology to develop the presented microfluidic platform may give rise to a new generation of biosensors capable of detecting low concentration samples from complex mixtures without the need for any sample pretreatment or labelling. The developed devices could have future applications in the field of early diagnosis of severe illnesses, e.g. early cancer detection.
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- 2014
23. Non periodic patterning of super-hydrophobic surfaces for the manipulation of few molecules
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E. Rondanina, Francesco Gentile, E. Di Fabrizio, Angelo Accardo, Daniele Di Mascolo, Marco Francardi, Patrizio Candeloro, Maria Laura Coluccio, F. De Angelis, S. Santoriello, Gentile, Francesco, Coluccio, M. L., Rondanina, E., Santoriello, S., Di Mascolo, D., Accardo, A., Francardi, M., De Angelis, F., Candeloro, P., and Di Fabrizio, E.
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Surface (mathematics) ,Atomic and Molecular Physics, and Optic ,Materials science ,Mathematical representation of surfaces ,Super-hydrophobic ,Evaporation ,Surfaces, Coatings and Film ,Nanotechnology ,02 engineering and technology ,01 natural sciences ,Mathematical representation of surface ,Rhodamine ,chemistry.chemical_compound ,0103 physical sciences ,Single molecule detection ,Molecule ,Electrical and Electronic Engineering ,Fourier transform infrared spectroscopy ,Spectroscopy ,SEIRA ,010302 applied physics ,Bio inspired materials ,SERS ,Surfaces ,Electronic, Optical and Magnetic Material ,Resolution (electron density) ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Atomic and Molecular Physics, and Optics ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Surface ,chemistry ,Bio inspired material ,Wetting ,0210 nano-technology - Abstract
Super-hydrophobic (SH) surfaces are bio-inspired, nanotechnology artifacts which feature a reduced friction coefficient whereby they can be used for a number of very practical applications including, on the medical side, the manipulation of biological solutions. These surfaces can be combined with bio-photonic devices to obtain an integrated lab-on-a-chip system where, on a first stage, the SH surface would vehicle or transport the analytes of interest into a small area and, on a second stage, the bio-sensors would permit, in that area, the detection of the solute with the resolution of a single molecule. This novel diagnostic modality offers realistic possibilities for the early detection of cancers. Nevertheless, as it stands, the device still suffers from the severe disadvantage that the exact final position of the solute, upon evaporation, is unpredictable, and thus the localization and recognition of few molecules would be impractical. Conventional SH surfaces typically comprise micro pillars combined to form a regular hexagonal motif. Here, the periodicity of those pillars was broken introducing artificial gradients of wettability over the surface. In doing so, some regions are rendered more hydrophilic than others and, on account of this, a solute would preferentially target these hydrophilic regions upon evaporation. In this work, such non regular geometries were realized and used to condense diluted Rhodamine solutions in a small area. Randomly distributed silver nano aggregates, conveniently positioned upon the micropillars, permitted the identification of few molecules using enhanced Fourier transform infrared spectroscopy (FTIR) spectroscopy.
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- 2013
- Full Text
- View/download PDF
24. Inclusion of gold nanoparticles in meso-porous silicon for the SERS analysis of cell adhesion on nano-structured surfaces
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Maria Laura Coluccio, S. De Vitis, P. Candeloro, Francesco Gentile, Gerardo Perozziello, G. Strumbo, E. Di Fabrizio, Coluccio, M. L, De Vitis, S., Strumbo, G., Candeloro, P., Perozziello, G., Di Fabrizio, E., and Gentile, Francesco
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0301 basic medicine ,Materials science ,Gold nanoparticle ,Atomic and Molecular Physics, and Optic ,Silicon ,chemistry.chemical_element ,Nano-topography ,Surfaces, Coatings and Film ,Nanotechnology ,Context (language use) ,02 engineering and technology ,Substrate (electronics) ,Condensed Matter Physic ,Porous silicon ,03 medical and health sciences ,symbols.namesake ,Electroless deposition ,Cell surface interaction ,Electrical and Electronic Engineering ,Cell adhesion ,Cell adhesion molecule ,SERS ,Electronic, Optical and Magnetic Material ,technology, industry, and agriculture ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Atomic and Molecular Physics, and Optics ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,030104 developmental biology ,chemistry ,Colloidal gold ,symbols ,0210 nano-technology ,Raman spectroscopy - Abstract
The study and the comprehension of the mechanism of cell adhesion and cell interaction with a substrate is a key point when biology and medicine meet engineering. This is the case of several biomedical applications, from regenerative medicine and tissue engineering to lab on chip and many others, in which the realization of the appropriate artificial surface allows the control of cell adhesion and proliferation.In this context, we aimed to design and develop a fabrication method of mesoporous (MeP) silicon substrates, doped with gold nanoparticles, in which we combine the capability of porous surfaces to support cell adhesion with the SERS capabilities of gold nanoparticles, to understand the chemical mechanisms of cell/surface interaction.MeP Si surfaces were realized by anodization of a Si wafer, creating the device for cell adhesion and growth. Gold nanoparticles were deposited on porous silicon by an electroless technique. We thus obtained devices with superior SERS capabilities, whereby cell activity may be controlled using Raman spectroscopy. MCF-7 breast cancer cells were cultured on the described substrates and SERS maps revealing the different expression and distribution of adhesion molecules were obtained by Raman spectroscopic analyses. Display Omitted designing and developing of a fabrication method of mesoporous (MeP) silicon substrates decorated with gold nanoparticlesthese devices are able to support cell adhesion and proliferation and simultaneously they works as SERS substratesSERS Raman analysis of cells cultured on MeP Si substrates provide indication about cells behaviour on them.
- Published
- 2016
25. Breaking the diffusion limit with super-hydrophobic delivery of molecules to plasmonic nanofocusing SERS structures
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Remo Proietti Zaccaria, Francesco Gentile, Gheorghe Cojoc, Federico Mecarini, Roberto Cingolani, Andrea Toma, Carlo Liberale, E. Di Fabrizio, F. De Angelis, Maria Laura Coluccio, Gerardo Perozziello, Gobind Das, Giovanni Cuda, Manola Moretti, Patrizio Candeloro, Luca Tirinato, Angelo Accardo, De Angelis, F., Gentile, Francesco, Mecarini, F., Das, G., Moretti, M., Candeloro, P., Coluccio, M. L., Cojoc, G., Accardo, A., Liberale, C., Zaccaria, R. P., Perozziello, G., Tirinato, L., Toma, A., Cuda, G., Cingolani, R., and Di Fabrizio, E.
- Subjects
Materials science ,Electronic, Optical and Magnetic Material ,Evaporation ,Nanotechnology ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Atomic and Molecular Physics, and Optics ,0104 chemical sciences ,Electronic, Optical and Magnetic Materials ,symbols.namesake ,symbols ,Molecule ,Diffusion limit ,0210 nano-technology ,Spectroscopy ,Raman spectroscopy ,Plasmon - Abstract
The detection of a few molecules in a highly diluted solution is of paramount interest in fields including biomedicine, safety and eco-pollution in relation to rare and dangerous chemicals. Nanosensors based on plasmonics are promising devices in this regard, in that they combine the features of high sensitivity, label-free detection and miniaturization. However, plasmonic-based nanosensors, in common with general sensors with sensitive areas on the scale of nanometres, cannot be used directly to detect molecules dissolved in femto- or attomolar solutions. In other words, they are diffusion-limited and their detection times become impractical at such concentrations. In this Article, we demonstrate, by combining super-hydrophobic artificial surfaces and nanoplasmonic structures, that few molecules can be localized and detected even at attomolar (10−18 mol l−1) concentration. Moreover, the detection can be combined with fluorescence and Raman spectroscopy, such that the chemical signature of the molecules can be clearly determined.
- Published
- 2011
- Full Text
- View/download PDF
26. Relating the rate of growth of metal nanoparticles to cluster size distribution in electroless deposition.
- Author
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Iatalese M, Coluccio ML, Onesto V, Amato F, Di Fabrizio E, and Gentile F
- Abstract
Electroless deposition on patterned silicon substrates enables the formation of metal nanomaterials with tight control over their size and shape. In the technique, metal ions are transported by diffusion from a solution to the active sites of an autocatalytic substrate where they are reduced as metals upon contact. Here, using diffusion limited aggregation models and numerical simulations, we derived relationships that correlate the cluster size distribution to the total mass of deposited particles. We found that the ratio ξ between the rates of growth of two different metals depends on the ratio γ between the rates of growth of clusters formed by those metals through the linearity law ξ = 14( γ - 1). We then validated the model using experiments. Different from other methods, the model derives k using as input the geometry of metal nanoparticle clusters, decoded by SEM or AFM images of samples, and a known reference., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)
- Published
- 2018
- Full Text
- View/download PDF
27. In vitro expansion of tumour cells derived from blood and tumour tissue is useful to redefine personalized treatment in non-small cell lung cancer patients.
- Author
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Malara NM, Givigliano F, Trunzo V, Macrina L, Raso C, Amodio N, Aprigliano S, Minniti AM, Russo V, Roveda L, Coluccio ML, Fini M, Voci P, Prati U, Di Fabrizio E, and Mollace V
- Subjects
- Aged, Aged, 80 and over, Biopsy, Carcinoma, Non-Small-Cell Lung therapy, Cell Cycle, Humans, Lung Neoplasms therapy, Lymphocytes, Tumor-Infiltrating pathology, Male, Middle Aged, Precision Medicine, Carcinoma, Non-Small-Cell Lung pathology, Lung Neoplasms pathology, Neoplastic Cells, Circulating
- Abstract
The clinical development of locally and advanced non-small cell lung cancer (NSCLC) suffers from a lack of biomarkers as a guide in the selection of optimal prognostic prediction. Circulating Tumour Cells (CTCs) are correlated to prognosis and show efficacy in cancer monitoring in patients. However, their enumeration alone might be inadequate; it might also be critical to understand the viability, the apoptotic state and the kinetics of these cells. Here, we report what we believe to be a new and selective approach to visually detect tumour specific CTCs. Firstly, using labelled human lung cancer cells, we detected a specific density interval in which NSCL-CTCs were concentrated. Secondly, to better characterize CTCs in respect to their heterogeneous composition and tumour reference, blood and tumour biopsy were performed on specimens taken from the same patient. The approach consisted in comparing phenotype profile of CTCs, and their progenitor Tumour Stem Cells, (TSCs). Moreover, NSCL-CTCs were cultivated in short-time human cultures to provide response to drug sensitivity. Our bimodal approach allowed to reveal two items. Firstly, that one part of a tumour, proximal to the bronchial structure, displays a predominance of CD133+. Secondly, specific NSCL-CTCs Epithelial Cell Adhesion Molecule (EpCAM)+CD29+ can be used as a negative prognostic factor as well the high expression of CTCs EpCAM+. These data were confirmed by drug-sensitivity tests, in vitro, and by the survival curves, in vivo.
- Published
- 2014
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