Search

Your search keyword '"Bortnik, V"' showing total 4 results
Start Over Author "Bortnik, V" Search Limiters Full Text
4 results on '"Bortnik, V"'

1. Loss of HPV type 16 E7 restores cGAS-STING responses in human papilloma virus-positive oropharyngeal squamous cell carcinomas cells

Author

Bortnik, V, Wu, M, Julcher, B, Salinas, A, Nikolic, I, Simpson, KJ, McMillan, NA, Idris, A, Bortnik, V, Wu, M, Julcher, B, Salinas, A, Nikolic, I, Simpson, KJ, McMillan, NA, and Idris, A

Abstract

Human papilloma viruses (HPV) are the main culprit in cervical and oropharyngeal cancers. HPV positive (+) cancers are regarded as 'oncogene addicted', displaying an absolute requirement for the continued expression of the oncogenes for their viability owing their survival, and thus making these genes salient targets for developing specific therapeutic agents. There is a strong association between HPV and oropharyngeal squamous cell carcinomas (OPSCC), a subset of head and neck cancers (HNCs). Alarmingly, HPV-associated OPSCC are on the rise globally, and the number of cases of HPV + OPSCCs surpasses that of cervical cancer in the USA. Here, we show that major HPV oncogenes, E6 and E7, are essential for the survival of HPV positive (+) OPSCCs, making these oncogenes salient targets for HPV-driven OPSCCs. HPV E7 is known to interact with STING, a component of the viral DNA-sensing cGAS-STING machinery which activates a pro-typical anti-viral type I interferon (IFN) response. Our recent work showed that E7 from HPV type 16 is responsible for the blockade of cGAS-STING responses in HPV + OPSCC cells. In this study, we show that CRISPR/Cas9-mediated loss of E7 from HPV + OPSCC cells, SCC2 and SCC104, restored cGAS-STING responses. Future work could involve HPV oncogene targeting leading to HPV + OPSCC tumour regression and that the combined use of STING agonists would induce favourable tumour clearance by activating appropriate anti-tumour responses.

Published
2021

2. «Погляд у майбутнє приладобудування», ХII Всеукраїнська науково-практична конференція студентів, аспірантів та молодих вчених

Author

Bortnik, V. A. and Borovytsky, V. M.

Subjects
KCF, minimal resolvable distance, secondary image, experiment, UAV, microscopic objective, ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION, Physics::Optics, Abbe theory, MOSSE, 681.5, primary image, spectrum, 535.015, OpenCV, diffraction grating, correlation filters
Abstract

The article presents the key stages of the history of the development of the wave theory of the formation of a diffraction grating image in a microscope system of coherent, non-coherent and partly coherent illumination.

Published
2019

3. Efficiency of Calibration of Distortion in UAV Vision Systems

Author

Bortnik, V. A. and Borovytsky, V. M.

Subjects
UAV, ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION, distortion calibration, image stitching, 681.5
Abstract

The UAV vision system is used for various tasks, such as cartography, the construction of 3d object models, the creation of gigapixel shots, and for navigation. Many of these tasks require image stitching. An obstacle to high-quality image stitching is the presence of aberrations in the lens of the camera, especially the distortion. Therefore, elimination of this aberration will allow more accurately and with less computer expenses to perform the task of image stitchin.

Published
2018

4. Loss of HPV type 16 E7 restores cGAS-STING responses in human papilloma virus-positive oropharyngeal squamous cell carcinomas cells.

Author

Bortnik V, Wu M, Julcher B, Salinas A, Nikolic I, Simpson KJ, McMillan NA, and Idris A

Subjects
Cell Line, DNA, Viral genetics, HeLa Cells, Head and Neck Neoplasms virology, Humans, Papillomaviridae classification, Squamous Cell Carcinoma of Head and Neck virology, Carcinoma, Squamous Cell virology, Host-Pathogen Interactions genetics, Membrane Proteins genetics, Nucleotidyltransferases genetics, Papillomaviridae genetics, Papillomavirus E7 Proteins genetics
Abstract

Human papilloma viruses (HPV) are the main culprit in cervical and oropharyngeal cancers. HPV positive (+) cancers are regarded as 'oncogene addicted', displaying an absolute requirement for the continued expression of the oncogenes for their viability owing their survival, and thus making these genes salient targets for developing specific therapeutic agents. There is a strong association between HPV and oropharyngeal squamous cell carcinomas (OPSCC), a subset of head and neck cancers (HNCs). Alarmingly, HPV-associated OPSCC are on the rise globally, and the number of cases of HPV + OPSCCs surpasses that of cervical cancer in the USA. Here, we show that major HPV oncogenes, E6 and E7, are essential for the survival of HPV positive (+) OPSCCs, making these oncogenes salient targets for HPV-driven OPSCCs. HPV E7 is known to interact with STING, a component of the viral DNA-sensing cGAS-STING machinery which activates a pro-typical anti-viral type I interferon (IFN) response. Our recent work showed that E7 from HPV type 16 is responsible for the blockade of cGAS-STING responses in HPV + OPSCC cells. In this study, we show that CRISPR/Cas9-mediated loss of E7 from HPV + OPSCC cells, SCC2 and SCC104, restored cGAS-STING responses. Future work could involve HPV oncogene targeting leading to HPV + OPSCC tumour regression and that the combined use of STING agonists would induce favourable tumour clearance by activating appropriate anti-tumour responses., Competing Interests: Declaration of competing interest Authors have no conflict of interest to declare., (Copyright © 2020. Published by Elsevier B.V.)

Published
2021
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results