1. Photosensitivity of Different Nanodiamond–PMO Nanoparticles in Two-Photon-Excited Photodynamic Therapy
- Author
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Nicolas Bondon, Denis Durand, Kamel Hadj-Kaddour, Lamiaa M. A. Ali, Rabah Boukherroub, Nadir Bettache, Magali Gary-Bobo, Laurence Raehm, Jean-Olivier Durand, Christophe Nguyen, Clarence Charnay, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Université de Montpellier (UM), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), We acknowledge the imaging facility MRI, member of the France-BioImaging national infrastructure, supported by the French National Research Agency (ANR-10-INBS-04, 'Investments for the future') and Erwan Olivieiro from the MEA platform, Université de Montpellier, for the STEM-EDX experiments. ANR-19-CE09-0034 MSN-2hv, ANR-19-CE09-0034,MSN-2hv,Matériaux théranostiques associant les fonctionnalités d'imagerie 2 photons et de thérapie photodynamique pour le ciblage de bactéries et le contrôle des infections lors de la cicatrisation(2019), ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and We acknowledge the imaging facility MRI, member of the France-BioImaging national infrastructure, supported by the French National Research Agency (ANR-10-INBS-04, 'Investments for the future') and Erwan Olivieiro from the MEA platform, Université de Montpellier, for the STEM-EDX experiments.ANR-19-CE09-0034 MSN-2hv
- Subjects
K. Ali ,R. Bettache ,theranostics ,J.-O. Nguyen ,Hadj-Kaddour ,Gary-Bobo ,K ,J.-O ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,L.M.A ,Bondon ,Raehm ,General Biochemistry, Genetics and Molecular Biology ,M ,[SPI]Engineering Sciences [physics] ,C ,et al nanodiamond ,Boukherroub ,N. Durand ,nanodiamond ,two-photon excitation ,mesoporous organosilica ,cancer cells ,Durand ,L. Durand ,Bettache ,Ecology, Evolution, Behavior and Systematics ,R ,D. Hadj-Kaddour ,Paleontology ,L.M.A. Boukherroub ,[CHIM.MATE]Chemical Sciences/Material chemistry ,N ,L ,N. Gary-Bobo ,Ali ,M. Raehm ,Space and Planetary Science ,C. et al nanodiamond two-photon excitation mesoporous organosilica theranostics cancer cells ,D ,[SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic ,Nguyen - Abstract
Background: In addition to their great optical properties, nanodiamonds (NDs) have recently proved useful for two-photon-excited photodynamic therapy (TPE-PDT) applications. Indeed, they are able to produce reactive oxygen species (ROS) directly upon two-photon excitation but not with one-photon excitation; Methods: Fluorescent NDs (FNDs) with a 100 nm diameter and detonation NDs (DNDs) of 30 nm were compared. In order to use the gems for cancer-cell theranostics, they were encapsulated in a bis(triethoxysilyl)ethylene-based (ENE) periodic mesoporous organosilica (PMO) shell, and the surface of the formed nanoparticles (NPs) was modified by the direct grafting of polyethylene glycol (PEG) and amino groups using PEG-hexyltriethoxysilane and aminoundecyltriethoxysilane during the sol–gel process. The NPs’ phototoxicity and interaction with MDA-MB-231 breast cancer cells were evaluated afterwards; Results: Transmission electronic microscopy images showed the formation of core–shell NPs. Infrared spectra and zeta-potential measurements confirmed the grafting of PEG and NH2 groups. The encapsulation of the NDs allowed for the imaging of cancer cells with NDs and for the performance of TPE-PDT of MDA-MB-231 cancer cells with significant mortality. Conclusions: Multifunctional ND@PMO core–shell nanosystems were successfully prepared. The NPs demonstrated high biocompatibility and TPE-PDT efficiency in vitro in the cancer cell model. Such systems hold good potential for two-photon-excited PDT applications.
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- 2022