70 results on '"Antonini S."'
Search Results
2. Yield and quality of curly kale grown using organic fertilizers
- Author
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VERRUMA-BERNARDI, M. R., PIMENTA, D. M., LEVRERO, G. R. R., FORTI, V. A., MEDEIROS, S. D. S. DE, CECCATO-ANTONINI, S. R., COVRE, E. A., FERREIRA, M. D., MORET, R., BERNARDI, A. C. DE. C., SALA, F. C., MARTA REGINA VERRUMA-BERNARDI, UFSCAR, DANIELLA MARTINS PIMENTA, UFSCAR, GABRIEL ROBERTO ROMANO LEVRERO, UFSCAR, VICTOR AUGUSTO FORTI, UFSCAR, SIMONE DANIELA SARTORIO DE MEDEIROS, UFSC, SANDRA REGINA CECCATO-ANTONINI, UFSCAR, ELIZABETE APARECIDA COVRE, UFSCAR, MARCOS DAVID FERREIRA, CNPDIA, RAISSA MORET, UFSCAR, ALBERTO CARLOS DE CAMPOS BERNARDI, CPPSE, and FERNANDO CÉSAR SALA, UFSCAR.
- Subjects
Brassica oleracea var acephala ,Minerals ,Weight loss ,Perda de massa ,Post-harvest ,Minerais ,Brassica Oleracea Acephala ,Pós-Colheita ,Color ,Harvesting ,Coloração da Couve - Abstract
Kale is a vegetable that has high nutrition content, and balanced fertilization is essential to ensure high yield and quality of agricultural products. Curly kale, less known than regular leaf kale, is a new possibility for consumption. However, little is known about the influence of the type of fertilization on nutritional characteristics. This study aims to evaluate the influence of using organic fertilizers on the productivity, microbiological, and physico-chemical characteristics of Darkibor hybrid curly kale. The treatments consisted of three sources of organic fertilizers, one of organomineral fertilizer, and the control (without fertilization). The highest productivity was observed for kale that was treated with fertilizer in the organomineral composition in all harvests. For microbiological analyses, there was no contamination in all treatments, following legislation. There was no significant difference between treatments for the physicochemical composition, highlighting the high levels of phenolic compounds and proteins in curly kale. There was no difference between treatments regarding the mineral content of the leaves. Organic and organomineral fertilizers made it possible to produce curly kale with adequate physicochemical composition, free from microbiological contamination and heavy metals. Made available in DSpace on 2021-06-17T13:01:10Z (GMT). No. of bitstreams: 1 Yield-Quality-Curly.pdf: 865387 bytes, checksum: eb9d25fe3bca3f59e4baa95f8c4f3bab (MD5) Previous issue date: 2021
- Published
- 2021
3. Growth hormone pharmacogenetics: the interactive effect of a microsatellite in the IGF1 promoter region with the GHR-exon 3 and −202 A/C IGFBP3 variants on treatment outcomes of children with severe GH deficiency
- Author
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Costalonga, E F, Antonini, S R R, Guerra-Junior, G, Coletta, R R D, Franca, M M, Braz, A F, Mendonca, B B, Arnhold, I J P, and Jorge, A A L
- Published
- 2012
- Full Text
- View/download PDF
4. Smoking, dietary calcium and vitamin D deficiency in women: a population-based study
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Morabia, A, Bernstein, MS, and Antonini, S
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- 2000
- Full Text
- View/download PDF
5. Growth hormone pharmacogenetics: the interactive effect of a microsatellite in the IGF1 promoter region with the GHR-exon 3 and -202 A/C IGFBP3 variants on treatment outcomes of children with severe GH deficiency
- Author
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Costalonga, E F, Antonini, S RR, Guerra-Junior, G, Coletta, R RD, Franca, M M, Braz, A F, Mendonca, B B, Arnhold, I JP, and Jorge, A AL
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- 2012
- Full Text
- View/download PDF
6. Juggling reference frames in the microworld Mak-Trace: the case of a student with MLD
- Author
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Baccaglini-Frank, A, Antonini, S, Robotti, E, and Santi, G
- Published
- 2014
7. I TUMORI IN ITALIA - RAPPORTO 2013: Tumori multipli = ITALIAN CANCER FIGURES - REPORT 2013: Multiple tumours
- Author
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Adamo, Ms, Alessi, D, Aletta, P, Amodio, R, Andreone, S, Angelin, T, Anghinoni, E, Annulli, Ml, Antonini, S, Artioli, Me, Autelitano, M, Balducci, C, Balottari, P, Baracco, M, Battisti, W, Bella, F, Bellatalla, C, Belluardo, C, Benatti, P, Benedetto, G, Benfatto, L, Bernazza, E, Bianconi, F, Biavati, P, Bidoli, E, Birri, S, Bizzoco, S, Bonelli, L, Bonini, A, Borciani, E, Bovo, E, Bozzani, F, Bozzeda, A, Braghiroli, B, Brucculeri, Ma, Brunori, V, Bucalo, G, Bucchi, L, Bugliarello, E, Bulatko, A, Busco, S, Busso, P, Buzzoni, C, Calabretta, L, Caldarella, A, Candela, G, Canu, L, Cappelletti, M, Caprara, L, Carboni, D, Carletti, N, Caroli, S, Carone, S, Cascio, Ma, Cascone, G, Casella, C, Castaing, M, Cecconami, L, Celesia, Mv, Cena, T, Cercato, Mc, Cesaraccio, R, Chiesa, R, Cirilli, C, Civaschi, A, Cocchioni, Mario, Codazzi, T, Cogno, R, Colamartini, A, Colanino Ziino, A, Cometti, I, Contiero, P, Contrino, Ml, Corbinelli, A, Cordaro, C, Corti, M, Costa, A, Costarelli, D, Cremone, L, Crocetti, E, Curatella, S, Cusimano, R, D'Alò, D, D'Angelo, S, Dal Cappello, T, Dal Cin, A, Dal Maso, L, Dall'Acqua, M, Dalsasso, F, Davini, C, De Dottori, M, De Maria, V, De Santis, E, De Valiere, E, Dei Tos AP, Demurtas, G, Devigli, E, Di Felice, E, di Grazia, L, Di Gregorio, C, Di Prima, A, Distefano, R, Doa, N, Domati, F, Fabiano, S, Facchinelli, G, Falcini, F, Falk, M, Fanetti, Ac, Fattoruso, S, Federico, M, Ferrari, L, Ferretti, S, Fidelbo, M, Filipazzi, L, Fiore, Ar, Fiori, G, Foca, F, Forgiarini, O, Frasca, G, Frassoldi, E, Frizza, J, Fusco, M, Gada, D, Garrone, E, Gasparotti, C, Gatti, L, Gaudiano, C, Gennaro, V, Gentilini, M, Gerevini, C, Ghilardi, S, Ghisleni, S, Giacomin, A, Giavazzi, L, Gilardi, F, Giorgetti, S, Giubelli, C, Giuliani, O, Giurdanella, Mc, Gola, G, Goldoni, Ca, Golizia, Mg, Grandi, L, Greco, A, Guarda, L, Guttadauro, A, Guzzinati, S, Iachetta, F, Iannelli, A, Ieni, A, Intrieri, T, Kaleci, S, La Rosa, F, Lando, C, Lavecchia, Am, Lazzarato, F, Leone, A, Leone, R, Lonati, F, Lottero, B, Lucchi, S, Luminari, S, Macci, L, Macerata, V, Madeddu, A, Maffei, S, Maghini, A, Magnani, C, Magnani, G, Magoni, M, Mameli, G, Mancini, S, Mancuso, P, Mangone, L, Manneschi, G, Mannino, R, Mannino, S, Marani, E, Mariani, F, Martorana, C, Marzola, L, Maspero, S, Maule, M, Mazzei, A, Mazzoleni, G, Mazzucco, G, Melcarne, A, Merletti, F, Michiara, M, Migliari, E, Minerba, S, Minicuzzi, A, Mizzi, M, Monetti, D, Morana, G, Moroni, E, Mosso, Ml, Muni, A, Mura, F, Natali, M, Nemcova, L, Nicita, C, Ocello, C, Paci, E, Pala, F, Palumbo, M, Panico, M, Pannozzo, F, Pascucci, Cristiana, Pastore, G, Patriarca, S, Pedroni, M, Pellegri, C, Perrotta, C, Pesce, P, Petrinelli, Am, Petrucci, C, Pezzarossi, A, Piffer, S, Pintori, N, Pirani, M, Pirino, D, Pironi, V, Ponz de Leon, M, Prandi, R, Prazzoli, R, Preto, L, Puleio, M, Puppo, A, Quaglia, A, Quarta, F, Quattrocchi, M, Raho, Am, Ramazzotti, V, Rashid, I, Ravaioli, A, Ravazzolo, B, Ravegnani, M, Reggiani Bonetti, L, Ribaudo, M, Rinaldi, E, Ricci, P, Rizzello, R, Rollo, Pc, Roncucci, L, Rosano, A, Rossi, F, Rossi, G, Rossi, M, Rossini, S, Rosso, S, Rudisi, G, Ruggeri, Mg, Russo, Ag, Russo, M, Sacchettini, C, Sacco, G, Sacerdote, C, Salvatore, S, Salvi, O, Sampietro, G, Sandrini, M, Santucci, C, Scheibel, M, Schiacchitano, S, Sciacca, S, Sciacchitano, C, Scuderi, T, Sechi, O, Seghini, P, Senatore, G, Serafini, G, Serraino, D, Sgargi, P, Sigona, A, Sini, Gm, Sobrato, I, Soddu, M, Solimene, C, Spano, F, Spata, E, Sperduti, I, Staiti, R, Stocco, C, Stracci, F, Sunseri, R, Sardo, As, Tagliabue, G, Tamburo, L, Tamburrino, S, Tanzarella, M, Terracini, B, Tessandori, R, Tisano, F, Tittarelli, A, Tognazzo, S, Torrisi, A, Traina, A, Trapani, C, Tschugguel, B, Tumino, R, Usala, M, Vacirca, S, Valerio, O, Valla, K, Varvarà, M, Vasquez, E, Vassante, B, Vattiato, R, Vercelli, M, Vercellino, Pc, Vicentini, M, Villa, M, Vitale, F, Vitale, Mf, Vitali, B, Vitarelli, Susanna, Zanchi, A, Zanetti, R, Zani, G, Zanier, L, Zappa, M, Zarcone, M, Zevola, A, Zucchetto, A, and Zucchi, A.
- Published
- 2013
8. Mesenchymal Stem Cell-Based Immunomodulation in Allogeneic Heterotopic Heart-Lung Transplantation
- Author
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Longoni, Biancamaria, Szilàgyi, E, Puviani, L, Mazzanti, B, Paoli, Gt, Urbani, S, Quaranta, P, Antonini, S, Tripodi, S, Caselli, C, Cintorino, M, Saccardi, R, Nardo, B, and Mosca, F.
- Subjects
Lung ,business.industry ,Mesenchymal stem cell ,Cell ,Hematopoietic stem cell ,In vitro ,medicine.anatomical_structure ,In vivo ,Immunology ,Cancer research ,medicine ,Cytokine secretion ,business ,Heart-Lung Transplantation - Abstract
Mesenchymal stem cells are able to differentiate in various cell lineages and they have shown immunomodulatory properties in vitro, altering the cytokine secretion profile of T helper, T effector and dendritic cells and stimulating natural killer cells towards an anti-inflammatory and tolerant phenotype. In vivo they prolong skin allograft survival and may decrease graft-versus-host disease after hematopoietic stem cell transplants. In this work we studied the effects of mesenchymal stem cell treatment in an allogeneic heterotopic heart-lung transplant model. The following experimental groups were formed: A) Control B) Immunosuppressive therapy (Cyclosporine A) C) Mesenchymal stem-cell intravenous infusion D) Mesenchymal stem-cell infusion plus immunosuppressive treatment. The infusion of mesenchymal stem cells improved the mean graft survival up to 14.5±3.7 days with respect to the control group (3±0.6 days). Treatment with Cyclosporine A plus mesenchymal stem cells (group D) produced a mean survival time of 18.25±4.9 days, and was not significantly different to the results for group B (21.75±3.5 days). Furthermore, in the immunosuppressive treatment and the mesenchymal stem cell treatment, histological analysis revealed a reduction in the grade of rejection in heart and lung grafts. This decrease was most significant in group D. In conclusion, mesenchymal stem cells alone or in combination with Cyclosporine A were able to prolong graft survival time. These data suggest that, in vivo, mesenchymal stem cells retain their ability, already shown in vitro, to suppress lymphocyte activation and proliferation.
- Published
- 2012
9. Anisakid nematodes in some fishes collected in the middle Adriatic sea: epidemiological analysis and molecular characterization of recombinant genotypes
- Author
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Burzacca, F., Antonini, S., D'Amelio, Stefano, Cavallero, Serena, Angeletti, M., and Favia, G.
- Published
- 2012
10. I tumori in Italia. Rapporto 2011: La sopravvivenza dei pazienti oncologici in Italia
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Fusco M, Airtum Working G. R. O. U. P., Buzzoni, C., Coviello, E., Rashid, I., Bianconi, F., Cuccaro, F., Castaing, M., Angelis, R., Giacomin, A., Guzzinati, S., Mosso, Ml, Pisani, P., Quaglia, A., Randi, G., Ramazzotti, V., Russo, A., Senatore, G., Stracci, F., Traina, A., Vercelli, M., Zarcone, M., Ferretti, S., Mazzoleni, G., Bellú, F., Tschugguel, B., Valiere, E., Facchinelli, G., Falk, M., Dal Cappello, T., Vercellino, Pc, Andreone, S., Busato, A., Marzola, L., Migliari, E., Carletti, N., Nenci, I., Crocetti, E., Caldarella, A., Corbinelli, A., Giusti, F., Intrieri, T., Manneschi, G., Nemcova, L., Romeo, G., Sacchettini, C., Zappa, M., Paci, E., Serraino, D., Angelin, T., Bidoli, E., Dal Maso, L., Dottori, M., Paoli, A., Santis, E., Forgiarini, O., Lise, M., Zucchetto, A., Zanier, L., Orengo, Ma, Casella, C., Marani, E., Puppo, A., Celesia, Mv, Cogno, R., Manenti, S., Garrone, E., Pannozzo, F., Busco, S., Cercato, Mc, Battisti, W., Sperduti, I., Macci, L., Bugliarello, E., Bernazza, E., Tamburo, L., Rossi, M., Curatella, S., Francesco, C., Tamburrino, S., Bisanti, L., Autelitano, M., Ghilardi, S., Leone, R., Filipazzi, L., Bonini, A., Giubelli, C., Federico, Massimo, Artioli, Me, Valla, K., Braghiroli, B., Cirilli, C., stefano luminari, Pirani, M., Ferrari, L., Bellatalla, C., Fusco, M., Panico, M., Perrotta, C., Vassante, B., Vitale, Mf, Michiara, M., Bozzani, F., Sgargi, P., Tumino, R., La Rosa MG, Cascone, G., Frasca, G., Giurdanella, Mc, Martorana, C., Morana, G., Nicita, C., Rollo, Pc, Ruggeri, Mg, Sigona, A., Spata, E., Vacirca, S., Mangone, L., Di Felice, E., Pezzarossi, A., Caroli, S., Pellegri, C., Vicentini, M., Storchi, C., Cavuto, S., Costa, J., Falcini, F., Colamartini, A., Bucchi, L., Balducci, C., Ravegnani, M., Vitali, B., Cordaro, C., Caprara, L., Giuliani, O., Giorgetti, S., Salvatore, S., Palumbo, M., Vattiato, R., Ravaioli, A., Foca, F., Rinaldi, E., Mancini, S., Tonelli, C., Amadori, M., Cremone, L., Iannelli, A., Zevola, A., Budroni, M., Cesaraccio, R., Pirino, D., Carboni, D., Fiori, G., Soddu, M., Mameli, G., Mura, F., Contrino, Ml, Madeddu, A., Tisano, F., Sciacca, S., Muni, A., Mizzi, M., Russo, M., Sacco, G., Aletta, P., Colanino Ziino, A., Tessandori, R., Fanetti, Ac, Maspero, S., Annulli, Ml, Moroni, E., Sanoja Gonzalez ME, Zanetti, R., Rosso, S., Patriarca, S., Prandi, R., Sobrato, I., Gilardi, F., Busso, P., Piffer, S., Gentilini, Ma, Battisti, L., Rizzello, R., Cappelletti, M., Moser, M., La Rosa, F., D Alò, D., Scheibel, M., Costarelli, D., Spano, F., Rossini, S., Santucci, C., Petrinelli, Am, Solimene, C., Brunori, V., Crosignani, P., Tagliabue, G., Contiero, P., Preto, L., Tittarelli, A., Maghini, A., Codazzi, T., Frassoldi, E., Gada, D., Costa, E., Di Grazia, L., Zambon, P., Baracco, M., Bovo, E., Dal Cin, A., Fiore, Ar, Greco, A., Monetti, D., Rosano, A., Stocco, C., Tognazzo, S., Donato, F., Limina, Rm, Adorni, A., Andreis, P., Zani, G., Piovani, F., Salvi, O., Puleio, M., Vitarelli, S., Antonini, S., Candela, G., Pappalardo, G., Scuderi, T., Lottero, B., Ribaudo, M., Ricci, P., Guarda, L., Gatti, L., Bozzeda, A., Dall Acqua, M., Pironi, V., Sutera Sardo, A., Mazzei, A., Sirianni, N., Lavecchia, Am, Mancuso, P., Usala, M., Pala, F., Sini, Gm, Pintori, N., Canu, L., Demurtas, G., Doa, N., Maurizio Ponz de Leon, Domati, Federica, Rossi, Giuseppina, Goldoni, Ca, Rossi, F., Gaetani, C., Piero Benatti, Luca Roncucci, Di Gregorio, C., Pedroni, Monica, Pezzi, A., Maffei, Stefania, Mariani, Francesco, Borsi, E., Carruba, G., Cusimano, R., Amodio, R., Dolcemascolo, C., Staiti, R., Pastore, G., Magnani, C., Terracini, B., Cena, T., Alessi, D., Baussano, I., Merletti, F., Maule, M., Macerata, V., Cocchioni, M., Pascucci, C., Gennaro, V., Lazzarotto, A., Benfatto, L., Mazzucco, G., and Montanaro, F.
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Survival ,cancer - Published
- 2011
11. Students' utilization schemes of pantographs for geometrical transformations: a first classification
- Author
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Martignone, Francesca and Antonini, S.
- Subjects
geometrical transformations ,Mathematical Machines ,utilization schemes ,pantographs ,cognitive processes - Published
- 2010
12. Exploring the Mathematical Machines for geometrical transformations: a cognitive analysis
- Author
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Martignone, Francesca and Antonini, S.
- Subjects
exploration processes ,pantographs ,utilization schemes - Published
- 2008
13. La dimostrazione per assurdo: un modello per un 'analisi cognitiva e didattica
- Author
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Antonini, S. and Mariotti, MARIA ALESSANDRA
- Subjects
Proof ,Indirect proo ,Proof , Argumentation ,Indirect proo,f Proof by contradiction , Proof by contraposition ,Argumentation ,Proof by contraposition ,f Proof by contradiction - Published
- 2006
14. New bioactive motifs and their use in functionalized self-assembling peptides for NSC differentiation and neural tissue engineering
- Author
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Gelain, F, Cigognini, D, Caprini, A, Silva, D, Colleoni, B, Donegá, M, Antonini, S, Cohen, B, Vescovi, A, Cohen, BE, VESCOVI, ANGELO LUIGI, Gelain, F, Cigognini, D, Caprini, A, Silva, D, Colleoni, B, Donegá, M, Antonini, S, Cohen, B, Vescovi, A, Cohen, BE, and VESCOVI, ANGELO LUIGI
- Abstract
Developing functionalized biomaterials for enhancing transplanted cell engraftment in vivo and stimulating the regeneration of injured tissues requires a multi-disciplinary approach customized for the tissue to be regenerated. In particular, nervous tissue engineering may take a great advantage from the discovery of novel functional motifs fostering transplanted stem cell engraftment and nervous fiber regeneration. Using phage display technology we have discovered new peptide sequences that bind to murine neural stem cell (NSC)-derived neural precursor cells (NPCs), and promote their viability and differentiation in vitro when linked to LDLK12 self-assembling peptide (SAPeptide). We characterized the newly functionalized LDLK12 SAPeptides via atomic force microscopy, circular dichroism and rheology, obtaining nanostructured hydrogels that support human and murine NSC proliferation and differentiation in vitro. One functionalized SAPeptide (Ac-FAQ), showing the highest stem cell viability and neural differentiation in vitro, was finally tested in acute contusive spinal cord injury in rats, where it fostered nervous tissue regrowth and improved locomotor recovery. Interestingly, animals treated with the non-functionalized LDLK12 had an axon sprouting/regeneration intermediate between Ac-FAQ-treated animals and controls. These results suggest that hydrogels functionalized with phage-derived peptides may constitute promising biomimetic scaffolds for in vitro NSC differentiation, as well as regenerative therapy of the injured nervous system. Moreover, this multi-disciplinary approach can be used to customize SAPeptides for other specific tissue engineering applications. © 2012 The Royal Society of Chemistry.
- Published
- 2012
15. Pericytes resident in postnatal skeletal muscle differentiate into muscle fibres and generate satellite cells
- Author
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Dellavalle, A, Maroli, G, Covarello, D, Azzoni, E, Innocenzi, A, Perani, L, Antonini, S, Sambasivan, R, Brunelli, S, Tajbakhsh, S, Cossu, G, Cossu, G., AZZONI, EMANUELE, BRUNELLI, SILVIA, Dellavalle, A, Maroli, G, Covarello, D, Azzoni, E, Innocenzi, A, Perani, L, Antonini, S, Sambasivan, R, Brunelli, S, Tajbakhsh, S, Cossu, G, Cossu, G., AZZONI, EMANUELE, and BRUNELLI, SILVIA
- Abstract
Skeletal muscle fibres form by fusion of mesoderm progenitors called myoblasts. After birth, muscle fibres do not increase in number but continue to grow in size because of fusion of satellite cells, the postnatal myogenic cells, responsible for muscle growth and regeneration. Numerous studies suggest that, on transplantation, non-myogenic cells also may contribute to muscle regeneration. However, there is currently no evidence that such a contribution represents a natural developmental option of these non-myogenic cells, rather than a consequence of experimental manipulation resulting in cell fusion. Here we show that pericytes, transgenically labelled with an inducible Alkaline Phosphatase CreERT2, but not endothelial cells, fuse with developing myofibres and enter the satellite cell compartment during unperturbed postnatal development. This contribution increases significantly during acute injury or in chronically regenerating dystrophic muscle. These data show that pericytes, resident in small vessels of skeletal muscle, contribute to its growth and regeneration during postnatal life.
- Published
- 2011
16. Transplantation of nanostructured composite scaffolds results in the regeneration of chronically injured spinal cords
- Author
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Gelain, F, Panseri, S, Antonini, S, Cunha, C, Donega, M, Lowery, J, Taraballi, F, Cerri, G, Montagna, M, Baldissera, F, Vescovi, A, VESCOVI, ANGELO LUIGI, Gelain, F, Panseri, S, Antonini, S, Cunha, C, Donega, M, Lowery, J, Taraballi, F, Cerri, G, Montagna, M, Baldissera, F, Vescovi, A, and VESCOVI, ANGELO LUIGI
- Abstract
The destruction and hollowing of entire tissue segments represent an insurmountable barrier to axonal regeneration and therapeutics in chronic spinal cord injury. To circumvent this problem, we engineered neural prosthetics, by assembling electrospun nanofibers and self-assembling peptides into composite guidance channels and transplanted them into the cysts of a postcontusive, chronic spinal cord injury rat model, also providing delivery of proregenerative cytokines. Six months later conspicuous cord reconstruction was observed. The cyst was replaced by newly formed tissue comprising neural and stromal cells. Nerve fibers were interspersed between and inside the guidance channels, spanning the lesion, amidst a well-developed vascular network, basal lamina, and myelin. This was accompanied by a significant improvement in the activity of ascending and descending motor pathways and the global locomotion score. Thus by engineering nanostructured matrices into neuroprosthetics, it is possible to recreate an anatomical, structural, and histological framework, which leads to the replacement of large, hollow tissue gaps in the chronically injured spinal cord, fostering axonal regeneration and neurological recovery.
- Published
- 2011
17. Polymorphisms of the adiponectin gene in gestational hypertension and pre-eclampsia
- Author
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Machado, J S R, primary, Palei, A C T, additional, Amaral, L M, additional, Bueno, A C, additional, Antonini, S R, additional, Duarte, G, additional, Tanus-Santos, J E, additional, Sandrim, V C, additional, and Cavalli, R C, additional
- Published
- 2013
- Full Text
- View/download PDF
18. RB1 deletion in gonadoblastoma in an XY female
- Author
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A. C. C. Barbosa, Carla Rosenberg, Antonini S, Angela Maria Vianna-Morgante, Angela Silva Barbosa, and Moreira-Filho Ac
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Tumor suppressor gene ,Adolescent ,Gonadoblastoma ,Gonadal dysgenesis ,Locus (genetics) ,Biology ,Polymerase Chain Reaction ,Loss of heterozygosity ,Genetics ,medicine ,Humans ,Genes, Retinoblastoma ,Genetics (clinical) ,In Situ Hybridization, Fluorescence ,Chromosome 13 ,Gonadal Dysgenesis, 46,XY ,Chromosomes, Human, Pair 13 ,Karyotype ,Exons ,Sequence Analysis, DNA ,medicine.disease ,Molecular biology ,eye diseases ,Chromosome Banding ,Testis determining factor ,Female ,Gene Deletion - Abstract
Cytogenetic studies of normal and tumor cells in a patient with gonadal dysgenesis and bilateral gonadoblastoma were performed. The karyotype was 46,XY in peripheral blood lymphocytes and skin fibroblasts. The conserved region of the SRY gene was detected by polymerase chain reaction amplification. Sequencing of this region did not reveal any alterations. A 46,XY chromosome constitution was observed in the right gonadoblastoma, but a partial deletion of chromosome 13 was present in the left tumor. This deletion included band 13q14, where the retinoblastoma gene is mapped. The study of the polymorphism of the variable number of tandem repeats region in intron 17 of the RB1 locus disclosed loss of heterozygosity in both the left tumor, which showed the deletion of chromosome 13, and in the right tumor, where no chromosome alterations of chromosome 13 were detected. In situ hybridization covering 130 kb of RB1 showed that a partial deletion of one of the RB1 alleles had occurred in the right tumor. Since the deletions affected different alleles in each tumor, independent events must have been involved in the development of the tumors. These findings point toward a significant role of RB1 in the development of gonadoblastoma.
- Published
- 1997
19. Growth hormone pharmacogenetics: the interactive effect of a microsatellite in the IGF1 promoter region with the GHR-exon 3 and −202 A/C IGFBP3 variants on treatment outcomes of children with severe GH deficiency
- Author
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Costalonga, E F, primary, Antonini, S R R, additional, Guerra-Junior, G, additional, Coletta, R R D, additional, Franca, M M, additional, Braz, A F, additional, Mendonca, B B, additional, Arnhold, I J P, additional, and Jorge, A A L, additional
- Published
- 2011
- Full Text
- View/download PDF
20. Pericytes resident in post-natal skeletal muscle differentiate into muscle fibers and enter the satellite cell pool
- Author
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Dellavalle, A., Maroli, G., Covarello, D., Azzoni, E., Innocenzi, A., Perani, L., Antonini, S., Sambasivan, R., Brunelli, S., Tajbakhsh, S., and Cossu, G.
- Published
- 2011
- Full Text
- View/download PDF
21. Il progetto 'Significati, congetture, dimostrazioni: dalle ricerche di base in didattica della matematica alle implicazioni curricolari (2005 – 2007)
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Antonini, S. and Maria Giuseppina Bartolini
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didattica della matematica ,congetture ,dimostrazioni ,significati
22. Management of acute dyarrhea: Current and future trends
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Franceschi, F., Scaldaferri, F., Riccioni, M. E., Casagranda, I., Forte, E., Gerardi, V., Cordischi, C., Antonini, S., Tortora, A., Di Rienzo, T., D Angelo, G., Giuseppe Merra, Costamagna, G., Zuccalà, G., and Gasbarrini, A.
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Adult ,Diarrhea ,Settore MED/12 - GASTROENTEROLOGIA ,Acute Disease ,Gelatin ,Humans ,dyarrhea ,Settore MED/49 ,Tannins - Abstract
Acute diarrhea is a very common symptom, which may recognize different causes and is basically the expression of an altered homeostasis of the bowel, which overcame current classifications. When approaching patients with acute diarrhea, we should firstly check body temperature and vital parameters and secondly provide a general medical examination mainly focused on the abdomen, in order to exclude surgical causes of diarrhea, such as acute appendicitis, diverticulitis, intestinal occlusion and others. Another important aspect is the assessment of the level of hydration in order to provide the right amount of fluids. There is no current indication for the administration of loperamide in infectious diarrhea, but there is a strong rationale for new class of drugs, which may be defined as "mucous regenerators", such as gelatin tannate. Further studies are needed on this matter in order to test the effect of gelatin tannate in adult patients with acute diarrhea.
23. Salivary cortisol as a tool for physiological studies and diagnostic strategies
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Castro M., Elias P.C.L., Martinelli Jr. C.E., Antonini S.R.R., Santiago L., and Moreira A.C.
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salivary cortisol ,ontogeny ,Cushing's syndrome ,Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
Salivary cortisol is an index of plasma free cortisol and is obtained by a noninvasive procedure. We have been using salivary cortisol as a tool for physiological and diagnostic studies, among them the emergence of circadian rhythm in preterm and term infants. The salivary cortisol circadian rhythm in term and premature infants was established between 8 and 12 postnatal weeks. In the preterm infants the emergence of circadian rhythm was parallel to the onset of sleep rhythm. We also studied the use of salivary cortisol for screening for Cushing's syndrome (CS) in control and obese outpatients based on circadian rhythm and the overnight 1 mg dexamethasone (DEX) suppression test. Salivary cortisol was suppressed to less than 100 ng/dl after 1 mg DEX in control and obese patients. A single salivary cortisol measurement at 23:00 h and again after 1 mg DEX above the 90th percentile of the obese group values had sensitivity and specificity of 93 and 93% (23:00 h), and 91 and 94% (after DEX), respectively. The sensitivity improved to 100% when we combined both parameters. We also studied 11 CS children and 21 age-matched primary obese children for whom salivary cortisol sensitivity and specificity were 100/95% (23:00 h), and 100/95% (1 mg DEX), respectively. Similar to adults, sensitivity and specificity of 100% were obtained by combining 23:00 h and 1 mg DEX. The measurement of salivary cortisol is a useful tool for physiological studies and for the diagnosis of CS in children and adults on an outpatient basis.
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- 2000
24. Development and validation of a prediction model for severe respiratory failure in hospitalized patients with SARS-Cov-2 infection: a multicenter cohort study (PREDI-CO study)
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Ciro Fulgaro, Ioannis Tzimas, Luigi Raumer, Marianna Meschiari, Marianna Menozzi, Gabriella Verucchi, Giada Rossini, Filippo Trapani, Giacomo Fornaro, Michela Semprini, Alessandra Cascavilla, Emanuele Campaci, Maddalena Giannella, Luigia Scudeller, Alessandro Zuccotti, Irid Baxhaku, Lucia Angelelli, Eleonora Zamparini, Annalisa Saracino, Alberto Zuppiroli, Cristina Basso, Elisabetta Pierucci, Agostino Rossi, Giulia Santangelo, Paolo Gaibani, Francesco Cristini, Francesca Volpato, Elisa Fronti, Giovanni Guaraldi, Alberto Sarti, Giorgio Legnani, Mattia Neri, Mauro Codeluppi, Adriana Badeanu, Giulio Virgili, Chiara Pironi, Lorenzo Marconi, Sara K. Tedeschi, Vidak Koprivika, Francesco Barchiesi, Luciano Attard, Matteo Rinaldi, Paola Laghetti, Stefano Antonini, Linda Bussini, Caterina Campoli, Giacomo Urbinati, Marco Merli, Nicholas Roncagli, Agnese Pratelli, Elena Rosselli Del Turco, Silvia Rapuano, Luca Guerra, Stefano Ianniruberto, Francesco Dell'Omo, Michele Bartoletti, Livia Pancaldi, Viola Guardigni, Fabio Tumietto, Giuseppe Sasdelli, Vito Marco Ranieri, Flovia Dauti, Giovanni Fasulo, Eugenia Francalanci, Nicola Dentale, Amalia Sanna Passino, Tommaso Zanaboni, Arianna Rubin, Davide Fiore Bavaro, Idina Zavatta, Massimo Puoti, Letizia Pasinelli, Maria Cristina Leoni, Pierluigi Viale, Oana Vatamanu, Elena Piccini, Renato Pascale, Cristina Mussini, Luca Esposito, Simona Coladonato, Alice Gori, Giulia Tesini, Lorenzo Badia, Mara D'Onofrio, Alberto Licci, Enrico Evangelisti, Guido Maria Liuzzi, Giacinto Pizzilli, Nicolò Rossi, Tommaso Tonetti, Marina Tadolini, Zeno Pasquini, Caterina Vocale, Bartoletti M., Giannella M., Scudeller L., Tedeschi S., Rinaldi M., Bussini L., Fornaro G., Pascale R., Pancaldi L., Pasquini Z., Trapani F., Badia L., Campoli C., Tadolini M., Attard L., Puoti M., Merli M., Mussini C., Menozzi M., Meschiari M., Codeluppi M., Barchiesi F., Cristini F., Saracino A., Licci A., Rapuano S., Tonetti T., Gaibani P., Ranieri V.M., Viale P., Raumer L., Guerra L., Tumietto F., Cascavilla A., Zamparini E., Verucchi G., Coladonato S., Rubin A., Ianniruberto S., Francalanci E., Volpato F., Virgili G., Rossi N., Del Turco E.R., Guardigni V., Fasulo G., Dentale N., Fulgaro C., Legnani G., Campaci E., Basso C., Zuppiroli A., Passino A.S., Tesini G., Angelelli L., Badeanu A., Rossi A., Santangelo G., Dauti F., Koprivika V., Roncagli N., Tzimas I., Liuzzi G.M., Baxhaku I., Pasinelli L., Neri M., Zanaboni T., Dell'Omo F., Vatamanu O., Gori A., Zavatta I., Antonini S., Pironi C., Piccini E., Esposito L., Zuccotti A., Urbinati G., Pratelli A., Sarti A., Semprini M., Evangelisti E., D'Onofrio M., Sasdelli G., Pizzilli G., Pierucci E., Rossini G., Vocale C., Marconi L., Leoni M.C., Fronti E., Guaraldi G., Bavaro D., Laghetti P., Bartoletti, M, Giannella, M, Scudeller, L, Tedeschi, S, Rinaldi, M, Bussini, L, Fornaro, G, Pascale, R, Pancaldi, L, Pasquini, Z, Trapani, F, Badia, L, Campoli, C, Tadolini, M, Attard, L, Puoti, M, Merli, M, Mussini, C, Menozzi, M, Meschiari, M, Codeluppi, M, Barchiesi, F, Cristini, F, Saracino, A, Licci, A, Rapuano, S, Tonetti, T, Gaibani, P, Ranieri, V, Viale, P, Raumer, L, Guerra, L, Tumietto, F, Cascavilla, A, Zamparini, E, Verucchi, G, Coladonato, S, Rubin, A, Ianniruberto, S, Francalanci, E, Volpato, F, Virgili, G, Rossi, N, Del Turco, E, Guardigni, V, Fasulo, G, Dentale, N, Fulgaro, C, Legnani, G, Campaci, E, Basso, C, Zuppiroli, A, Passino, A, Tesini, G, Angelelli, L, Badeanu, A, Rossi, A, Santangelo, G, Dauti, F, Koprivika, V, Roncagli, N, Tzimas, I, Liuzzi, G, Baxhaku, I, Pasinelli, L, Neri, M, Zanaboni, T, Dell'Omo, F, Vatamanu, O, Gori, A, Zavatta, I, Antonini, S, Pironi, C, Piccini, E, Esposito, L, Zuccotti, A, Urbinati, G, Pratelli, A, Sarti, A, Semprini, M, Evangelisti, E, D'Onofrio, M, Sasdelli, G, Pizzilli, G, Pierucci, E, Rossini, G, Vocale, C, Marconi, L, Leoni, M, Fronti, E, Guaraldi, G, Bavaro, D, and Laghetti, P
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0301 basic medicine ,Male ,Logistic regression ,prognostic tool ,0302 clinical medicine ,Risk Factors ,Positive predicative value ,Severe acute respiratory syndrome coronavirus 2 ,030212 general & internal medicine ,Child ,Aged, 80 and over ,Framingham Risk Score ,Coronavirus disease 2019 ,Respiratory distress ,Lactate dehydrogenase ,General Medicine ,Middle Aged ,Prognosis ,Hospitalization ,Infectious Diseases ,Italy ,Child, Preschool ,Female ,Coronavirus Infections ,Respiratory Insufficiency ,Cohort study ,Microbiology (medical) ,Adult ,medicine.medical_specialty ,Respiratory rate ,Adolescent ,COVID-19 ,SARS-CoV-2 ,severe respiratory failure ,030106 microbiology ,Pneumonia, Viral ,Risk Assessment ,Sensitivity and Specificity ,Article ,03 medical and health sciences ,Betacoronavirus ,Young Adult ,Age ,Internal medicine ,medicine ,Humans ,Obesity ,Pandemics ,Aged ,Retrospective Studies ,business.industry ,Reproducibility of Results ,Retrospective cohort study ,Logistic Models ,Respiratory failure ,Multivariate Analysis ,business ,C-reactive proteine - Abstract
Objectives: We aimed to develop and validate a risk score to predict severe respiratory failure (SRF) among patients hospitalized with coronavirus disease-2019 (COVID-19). Methods: We performed a multicentre cohort study among hospitalized (>24 hours) patients diagnosed with COVID-19 from 22 February to 3 April 2020, at 11 Italian hospitals. Patients were divided into derivation and validation cohorts according to random sorting of hospitals. SRF was assessed from admission to hospital discharge and was defined as: SpO2 30 breaths/min or respiratory distress. Multivariable logistic regression models were built to identify predictors of SRF, β-coefficients were used to develop a risk score. Trial Registration NCT04316949. Results: We analysed 1113 patients (644 derivation, 469 validation cohort). Mean (±SD) age was 65.7 (±15) years, 704 (63.3%) were male. SRF occurred in 189/644 (29%) and 187/469 (40%) patients in the derivation and validation cohorts, respectively. At multivariate analysis, risk factors for SRF in the derivation cohort assessed at hospitalization were age ≥70 years (OR 2.74; 95% CI 1.66–4.50), obesity (OR 4.62; 95% CI 2.78–7.70), body temperature ≥38°C (OR 1.73; 95% CI 1.30–2.29), respiratory rate ≥22 breaths/min (OR 3.75; 95% CI 2.01–7.01), lymphocytes ≤900 cells/mm3 (OR 2.69; 95% CI 1.60–4.51), creatinine ≥1 mg/dL (OR 2.38; 95% CI 1.59–3.56), C-reactive protein ≥10 mg/dL (OR 5.91; 95% CI 4.88–7.17) and lactate dehydrogenase ≥350 IU/L (OR 2.39; 95% CI 1.11–5.11). Assigning points to each variable, an individual risk score (PREDI-CO score) was obtained. Area under the receiver-operator curve was 0.89 (0.86–0.92). At a score of >3, sensitivity, specificity, and positive and negative predictive values were 71.6% (65%–79%), 89.1% (86%–92%), 74% (67%–80%) and 89% (85%–91%), respectively. PREDI-CO score showed similar prognostic ability in the validation cohort: area under the receiver-operator curve 0.85 (0.81–0.88). At a score of >3, sensitivity, specificity, and positive and negative predictive values were 80% (73%–85%), 76% (70%–81%), 69% (60%–74%) and 85% (80%–89%), respectively. Conclusion: PREDI-CO score can be useful to allocate resources and prioritize treatments during the COVID-19 pandemic.
- Published
- 2020
25. Prevention of recurrent respiratory infections
- Author
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Katia Perruccio, Giulia Trippella, Martina Ciarcià, Paolo Becherucci, Alberto Villani, Marco Zecca, Valeria Caldarelli, Sergio Bottero, Maria Laura Panatta, Diego Peroni, Giuseppe Di Mauro, Angela Pasinato, Lorenzo Pignataro, Sofia D’Elios, Diletta Valentini, Maurizio de Martino, Guido Morbin, Fabio Cardinale, Vito Leonardo Miniello, Massimo Pifferi, Luciana Indinnimeo, Luisa Galli, Marco Antonio Motisi, Francesca Santamaria, Gian Luigi Marseglia, Francesco Macrì, Chiara Tersigni, Anna Teresa Palamara, Michele Miraglia Del Giudice, Guido Castelli Gattinara, Paolo Biasci, Emanuela Sitzia, Renato Cutrera, Elena Chiappini, Andrea Lo Vecchio, Roberto Mattina, Daniele Ciofi, Claudio Vicini, Sandro Valentini, Paola Marchisio, Attilio Varricchio, Mattia Doria, Sara Torretta, Irene Trambusti, Andrea Novelli, Barbara Bortone, Giorgio Piacentini, Maria Carmen Verga, Sara Antonini, Chiappini, E., Santamaria, F., Marseglia, G. L., Marchisio, P., Galli, L., Cutrera, R., de Martino, M., Antonini, S., Becherucci, P., Biasci, P., Bortone, B., Bottero, S., Caldarelli, V., Cardinale, F., Gattinara, G. C., Ciarcia, M., Ciofi, D., D'Elios, S., Di Mauro, G., Doria, M., Indinnimeo, L., Lo Vecchio, A., Macri, F., Mattina, R., Miniello, V. L., del Giudice, M. M., Morbin, G., Motisi, M. A., Novelli, A., Palamara, A. T., Panatta, M. L., Pasinato, A., Peroni, D., Perruccio, K., Piacentini, G., Pifferi, M., Pignataro, L., Sitzia, E., Tersigni, C., Torretta, S., Trambusti, I., Trippella, G., Valentini, D., Valentini, S., Varricchio, A., Verga, M. C., Vicini, C., Zecca, M., and Villani, A.
- Subjects
Pneumococcal Vaccine ,Complementary Therapies ,medicine.medical_specialty ,Prebiotic ,Review ,Probiotic ,Recurrent respiratory infection ,Antioxidants ,Adenoidectomy ,Pneumococcal Vaccines ,Adjuvants, Immunologic ,Quality of life ,Recurrence ,medicine ,Antibiotic Prophylaxi ,Respiratory Tract Infection ,Humans ,Recurrent respiratory infections ,Thiazolidine ,Hyaluronic Acid ,Child ,Intensive care medicine ,Respiratory Tract Infections ,Children ,Administration, Intranasal ,Tonsillectomy ,Maternal and child health ,business.industry ,Probiotics ,Prevention ,Vitamins ,Antibiotic Prophylaxis ,Settore MED/38 ,Complete resolution ,Pyrrolidonecarboxylic Acid ,Algorithm ,Prebiotics ,Immune system ,Complementary Therapie ,Influenza Vaccines ,Resveratrol ,Thiazolidines ,Antioxidant ,Influenza Vaccine ,business ,Algorithms ,Human - Abstract
Recurrent respiratory infections (RRIs) are a common clinical condition in children, in fact about 25% of children under 1 year and 6% of children during the first 6 years of life have RRIs. In most cases, infections occur with mild clinical manifestations and the frequency of episodes tends to decrease over time with a complete resolution by 12 years of age. However, RRIs significantly reduce child and family quality of life and lead to significant medical and social costs. Despite the importance of this condition, there is currently no agreed definition of the term RRIs in the literature, especially concerning the frequency and type of infectious episodes to be considered. The aim of this consensus document is to propose an updated definition and provide recommendations with the intent of guiding the physician in the complex process of diagnosis, management and prevention of RRIs. Supplementary Information The online version contains supplementary material available at 10.1186/s13052-021-01150-0.
- Published
- 2021
26. Singularity-free and non-chaotic inhomogeneous Mixmaster in polymer representation for the volume of the universe
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Stefano Antonini, Giovanni Montani, Antonini, S., and Montani, G.
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Nuclear and High Energy Physics ,polymer ,Big Bounce ,Inhomogeneous ,Mixmaster ,Polymer ,FOS: Physical sciences ,mixmaster ,inhomogeneous ,big bounce ,Inhomogeneou ,Cosmological constant ,General Relativity and Quantum Cosmology (gr-qc) ,01 natural sciences ,General Relativity and Quantum Cosmology ,De Sitter universe ,0103 physical sciences ,010306 general physics ,Scale factor (cosmology) ,Loop quantum cosmology ,Physics ,010308 nuclear & particles physics ,lcsh:QC1-999 ,Classical mechanics ,Gravitational singularity ,Scalar field ,lcsh:Physics ,Scalar curvature - Abstract
We analyze the semiclassical polymer dynamics of the inhomogeneous Mixmaster model by choosing the cubed scale factor as the discretized configurational variable, while the anisotropies remain pure Einsteinian variables. Such a modified theory of gravity should be regarded as the appropriate framework to describe the behavior of quantum mean values, both in polymer quantum mechanics and in Loop Quantum Cosmology. We first construct the generalized Kasner solution, including a massless scalar field and a cosmological constant in the dynamics. They account for a quasi-isotropization, inflationary-like mechanism. The resulting scenario links a singularity-free Kasner-like regime with a homogeneous and isotropic de Sitter phase. Subsequently, we investigate the role of the three-dimensional scalar curvature, demonstrating that a bounce of the point-universe against the potential walls can always occur within the polymer framework, also in the presence of a scalar field. However, the absence of the singularity implies that the curvature is bounded. Therefore, the point-universe undergoes an oscillatory regime until it oversteps the potential walls (if the Big Bounce is not reached before). After that, a final stable Kasner-like regime will last all the way toward the Big Bounce. Thus, the present study demonstrates that, as soon as a discretization of the volume of the universe is performed, the generic cosmological solution is non-chaotic and free from singularities. It is likely that the same result can be achieved also in the loop quantum cosmology approach., 11 pages, 4 figures, to appear on Physics Letters B
- Published
- 2019
27. Study of adhesion and migration dynamics in ubiquitin E3A ligase (UBE3A)-silenced SYSH5Y neuroblastoma cells by micro-structured surfaces
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Roberta Mezzena, Federico Cremisi, Martin Scheffner, Cecilia Masciullo, Sara Antonini, Marco Cecchini, Ilaria Tonazzini, Mezzena, R., Masciullo, C., Antonini, S., Cremisi, F., Scheffner, M., Cecchini, M., and Tonazzini, I.
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Materials science ,SH-SY5Y ,Mechanotransduction ,Surface Properties ,Ubiquitin-Protein Ligases ,Integrin ,Bioengineering ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Extracellular matrix ,Focal adhesion ,Settore BIO/06 - Anatomia Comparata e Citologia ,Neuroblastoma ,Contact guidance ,Cell Movement ,ddc:570 ,Cell Line, Tumor ,Cell Adhesion ,UBE3A ,Humans ,General Materials Science ,Gene Silencing ,Electrical and Electronic Engineering ,Paxillin ,mechanotransduction ,Neurons ,Micrograting ,Focal Adhesions ,biology ,Mechanical Engineering ,General Chemistry ,Transfection ,021001 nanoscience & nanotechnology ,Nanostructures ,0104 chemical sciences ,Cell biology ,contact guidance ,micrograting ,Mechanics of Materials ,biology.protein ,Angelman Syndrome ,0210 nano-technology ,Neuronal Differentiation - Abstract
During neuronal development, neuronal cells read extracellular stimuli from the micro/nano-environment within which they exist, retrieving essential directionality and wiring information. Here, focal adhesions (FAs-protein clusters anchoring integrins to cytoskeleton) act as sensors, by integrating signals from both the extracellular matrix environment and chemotactic factors, contributing to the final neuronal pathfinding and migration. In the processes that orchestrate neuronal development, the important function of ubiquitin E3A ligase (UBE3A) is emerging. UBE3A has crucial functions in the brain and changes in its expression levels lead to neurodevelopmental disorders: the lack of UBE3A leads to Angelman syndrome (AS, OMIN 105830), while its increase causes autisms (Dup15q-autism). By using nano/micro-structured anisotropic substrates we previously showed that UBE3A-deficient neurons have deficits in contact guidance (Tonazzini et al, Mol Autism 2019). Here, we investigate the adhesion and migration dynamics of UBE3A-silenced SH-SY5Y neuroblastoma cells in vitro by exploiting nano/micro-grooved substrates. We analyze the molecular processes regulating the development of FAs by transfection with EGFP-vector encoding for paxillin, a protein of FA clusters, and by live-cell total-internal-reflection-fluorescence microscopy. We show that UBE3A-silenced SH-SY5Y cells have impaired FA morphological development and pathway activation, which lead to a delayed adhesion and also explain the defective contact guidance in response to directional topographical stimuli. However, UBE3A-silenced SH-SY5Y cells show an overall normal migration behavior, in terms of speed and ability to follow the GRs directional stimulus. Only the collective cell migration upon cell gaps was slightly delayed for UBE3Ash SHs. Overall, the deficits of UBE3Ash SHS-SY5Y cells in FA maturation/sensing and in collective migration may have patho-physiological implications, in AS condition, considering the much more complex stimuli that neurons find in vivo during the neurodevelopment.
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- 2020
28. Development, embryonic genome activity and mitochondrial characteristics of bovine–pig inter-family nuclear transfer embryos
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Irina Lagutina, Helena Fulka, Josef Fulka, Silvia Colleoni, Dario Brunetti, Giovanna Lazzari, Cesare Galli, Stefania Antonini, Tiziana A. L. Brevini, Fulvio Gandolfi, Lagutina I, Fulka H, Brevini TA, Antonini S, Brunetti D, Colleoni S, Gandolfi F, Lazzari G, Fulka J Jr, and Galli C
- Subjects
INTERSPECIES ,Male ,Cytoplasm ,Nuclear Transfer Techniques ,Embryology ,Swine ,Nucleolus ,Embryonic Development ,RNA polymerase II ,Collagen Type VI ,Polymerase Chain Reaction ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Endocrinology ,Pregnancy ,RNA Polymerase I ,RNA polymerase ,RNA polymerase I ,Animals ,Gene ,030304 developmental biology ,Cell Nucleus ,0303 health sciences ,030219 obstetrics & reproductive medicine ,biology ,SCNT ,Obstetrics and Gynecology ,RNA ,DNA ,Cell Biology ,Blastomere ,GENOME ACTIVATION ,Embryo, Mammalian ,Molecular biology ,Mitochondria ,Reproductive Medicine ,chemistry ,embryonic structures ,biology.protein ,Somatic cell nuclear transfer ,Cattle ,Female ,RNA Polymerase II - Abstract
The best results of inter-species somatic cell nuclear transfer (iSCNT) in mammals were obtained using closely related species that can hybridise naturally. However, in the last years, many reports describing blastocyst development following iSCNT between species with distant taxonomical relations (inter-classes, inter-order and inter-family) have been published. This indicates that embryonic genome activation (EGA) in xeno-cytoplasm is possible, albeit very rarely. Using a bovine–pig (inter-family) iSCNT model, we studied the basic characteristics of EGA: expression and activity of RNA polymerase II (RNA Pol II), formation of nucleoli (as an indicator of RNA polymerase I (RNA Pol I) activity), expression of the key pluripotency geneNANOGand alteration of mitochondrial mass. In control embryos (obtained by IVF or iSCNT), EGA was characterised by RNA Pol II accumulation and massive production of poly-adenylated transcripts (detected with oligo dT probes) in blastomere nuclei, and formation of nucleoli as a result of RNA Pol I activity. Conversely, iSCNT embryos were characterised by the absence of accumulation and low activity of RNA Pol II and inability to form active mature nucleoli. Moreover, in iSCNT embryos,NANOGwas not expressed, and mitochondria mass was significantly lower than in intra-species embryos. Finally, the complete developmental block at the 16–25-cell stage for pig–bovine iSCNT embryos and at the four-cell stage for bovine–pig iSCNT embryos strongly suggests that EGA is not taking place in iSCNT embryos. Thus, our experiments clearly demonstrate poor nucleus–cytoplasm compatibility between these animal species.
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- 2010
29. Sub-micron lateral topography affects endothelial migration by modulation of focal adhesion dynamics
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Sara Antonini, Sandro Meucci, Mirko Klingauf, Fabio Beltram, Aldo Ferrari, Dimos Poulikakos, Marco Cecchini, Emanuela Jacchetti, Antonini, S, Meucci, S, Jacchetti, Emanuela, Klingauf, Mirko, Beltram, Fabio, Poulikakos, D, Cecchini, M, and Ferrari, A.
- Subjects
Cell physiology ,Surface Properties ,Cell ,Biomedical Engineering ,Bioengineering ,Nanotechnology ,Biocompatible Materials ,Biology ,migration ,Regenerative Medicine ,Mechanotransduction, Cellular ,Biomaterials ,Focal adhesion ,In vivo ,Cell Movement ,Cell polarity ,Materials Testing ,medicine ,Human Umbilical Vein Endothelial Cells ,Humans ,focal adhesion ,Mechanotransduction ,Focal Adhesions ,Rational design ,Endothelial Cells ,contact guidance ,medicine.anatomical_structure ,endothelial cell ,nanograting ,Biophysics ,Function (biology) - Abstract
Through the interaction with topographical features, endothelial cells tune their ability to populate target substrates, both in vivo and in vitro. Basal textures interfere with the establishment and maturation of focal adhesions (FAs) thus inducing specific cell-polarization patterns and regulating a plethora of cell activities that govern the overall endothelial function. In this study, we analyze the effect of topographical features on FAs in primary human endothelial cells. Reported data demonstrate a functional link between FA dynamics and cell polarization and spreading on structured substrates presenting variable lateral feature size. Our results reveal that gratings with 2 µm lateral periodicity maximize contact guidance. The effect is linked to the dynamical state of FAs. We argue that these results are readily applicable to the rational design of active surfaces at the interface with the blood stream.
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- 2015
30. Pericytes resident in postnatal skeletal muscle differentiate into muscle fibres and generate satellite cells
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Giulio Cossu, Ramkumar Sambasivan, Shahragim Tajbakhsh, Laura Perani, Emanuele Azzoni, G. Maroli, Silvia Brunelli, Anna Innocenzi, Diego Covarello, Stefania Antonini, Arianna Dellavalle, Dellavalle, A, Maroli, G, Covarello, D, Azzoni, E, Innocenzi, A, Perani, L, Antonini, S, Sambasivan, R, Brunelli, S, Tajbakhsh, S, and Cossu, G
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Cellular differentiation ,General Physics and Astronomy ,Mice, Transgenic ,Biology ,Real-Time Polymerase Chain Reaction ,General Biochemistry, Genetics and Molecular Biology ,Muscle hypertrophy ,Mice ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Animals ,Regeneration ,Myocyte ,Progenitor cell ,Muscle, Skeletal ,Pericyte ,030304 developmental biology ,0303 health sciences ,Multidisciplinary ,Mesoangioblast ,Reverse Transcriptase Polymerase Chain Reaction ,Animal ,Regeneration (biology) ,Skeletal muscle ,Cell Differentiation ,General Chemistry ,Immunohistochemistry ,Molecular biology ,Cell biology ,Transplantation ,medicine.anatomical_structure ,Pericytes ,030217 neurology & neurosurgery - Abstract
Skeletal muscle fibres form by fusion of mesoderm progenitors called myoblasts. After birth, muscle fibres do not increase in number but continue to grow in size because of fusion of satellite cells, the postnatal myogenic cells, responsible for muscle growth and regeneration. Numerous studies suggest that, on transplantation, non-myogenic cells also may contribute to muscle regeneration. However, there is currently no evidence that such a contribution represents a natural developmental option of these non-myogenic cells, rather than a consequence of experimental manipulation resulting in cell fusion. Here we show that pericytes, transgenically labelled with an inducible Alkaline Phosphatase CreERT2, but not endothelial cells, fuse with developing myofibres and enter the satellite cell compartment during unperturbed postnatal development. This contribution increases significantly during acute injury or in chronically regenerating dystrophic muscle. These data show that pericytes, resident in small vessels of skeletal muscle, contribute to its growth and regeneration during postnatal life.
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- 2011
31. Evaluation of Anorexia in Cancer and Its Association with Autonomic Nervous System Activity Assessed by Heart Rate Variability.
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Molfino A, Gallicchio C, Imbimbo G, Melena M, Antonini S, Gigante A, and Muscaritoli M
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- Humans, Heart Rate physiology, Autonomic Nervous System, Electrocardiography, Ambulatory, Anorexia etiology, Neoplasms complications
- Abstract
Alterations in the central nervous system in cancer patients are pivotal in determining appetite dysregulation and body weight loss (BWL). Autonomic nervous system activity was tested by measuring heart rate variability (HRV) in cancer patients presenting with anorexia. We considered inpatients with different types of cancer and investigated anorexia using their FAACT scores. HRV was evaluated by a three-channel Holter ECG. The domains of low frequencies (LF, sympathetic activity) and high frequencies (HF, parasympathetic activity) were calculated. Also, SDNN (autonomic activity) and RMSSD (parasympathetic activity) were assessed. We enrolled 56 patients with cancer and 23 controls. In cancer patients, RMSSD and SDNN were lower than in controls ( p < 0.001 and p = 0.009). Sympathetic activity (LF nu) was lower in cancer patients than in controls ( p = 0.023), including sympathovagal balance (LF/HF nu ratio) ( p = 0.025). RMSSD was reduced in anorexic ( p < 0.001) and non-anorexic ( p = 0.003) cancer patients compared to controls. The SDNN was lower in anorexic cancer patients than in non-anorexic cancer patients ( p = 0.025), and it was lower in anorexic cancer patients than in controls ( p = 0.001). LF nu was lower in anorexic cancer patients than in controls ( p = 0.015), as was LF/HF ( p = 0.031). SDNN was negatively correlated with BWL in the cancer group (rho = -0.40; p = 0.007). Our data support the hypothesis that autonomic nervous system dysregulation exists in patients with cancer presenting with anorexia, with implications for its diagnosis and treatment.
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- 2023
- Full Text
- View/download PDF
32. ACTH Stimulation Test for the Diagnosis of Secondary Adrenal Insufficiency: Light and Shadow.
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Birtolo MF, Antonini S, Saladino A, Zampetti B, Lavezzi E, Chiodini I, Mazziotti G, Lania AGA, and Cozzi R
- Abstract
Secondary Adrenal Insufficiency (SAI) is a condition characterized by inappropriately low ACTH secretion due to a disease or injury to the hypothalamus or the pituitary. The evaluation when suspected is often challenging for the non-specific symptoms, the rarity of the disease, and the pitfalls associated with laboratory tests. A prompt and correct diagnosis of SAI is essential because although an adequate hormonal replacement therapy could be lifesaving, inappropriate life-long therapy with steroids can be harmful. The gold standard test for assessing the hypothalamus-pituitary-adrenal axis (HPA) is the insulin tolerance test (ITT), but due to safety issues is not widely used. Conversely, the ACTH stimulation test is a safer and well-tolerated tool for SAI diagnosis. However, data about its diagnostic accuracy show great variability due to both technical and interpretative aspects, such as dose, route of administration, the timing of the test, and assay used for cortisol measurements. Consequently, the clinical background of the patient and the pretest probability of HPA axis impairment become of paramount importance. We aimed to summarize the recent literature evidence in the conduction and interpretation of the ACTH stimulation test for the diagnosis of SAI to provide updated insights on its correct use in clinical practice.
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- 2023
- Full Text
- View/download PDF
33. Hemophagocytic lymphohistiocytosis in gastric cancer: A rare syndrome for the oncologist. Case report and brief review.
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Monti M, Marconi G, Ambrosini-Spaltro A, Gallio C, Ghini V, Esposito L, Antonini S, Montanari D, and Frassineti GL
- Abstract
Hemophagocytic lymphohistiocytosis (HLH) is a rare and life-threatening condition characterized by uncontrolled activation of the immune system. HLH is a reactive mononuclear phagocytic response that occurs in association with a constellation of conditions such as malignancies and infections. The clinical diagnosis of HLH remains challenging because HLH can present with symptoms that significantly overlap with other causes of cytopenia, such as sepsis, autoimmune diseases, hematological cancers, and multiorgan failure. A 50-year-old man went to the emergency room (ER) for hyperchromic urine, melena, gingivorrhagia, and spontaneous abdominal wall hematomas. The first blood tests showed severe thrombocytopenia, alteration of the INR, and consumption of fibrinogen, and therefore, a diagnosis of disseminated intravascular coagulation (DIC) was made. A bone marrow aspirate showed numerous images of hemophagocytosis. With the suspicion of immune-mediated cytopenia, oral etoposide, intravenous immunoglobulin, and intravenous methylprednisolone were administered. Then, a diagnosis of gastric carcinoma was performed with a lymph node biopsy and gastroscopy. On the 30th day, the patient was transferred to the oncology ward of another hospital. On admission, he had serious piastrinopenia, anemia, hypertriglyceridemia, and hyperferritinemia. He was supported with a platelet transfusion and underwent a bone biopsy that showed a picture compatible with myelophthisis from diffuse medullary localization of a carcinoma of gastric origin. A diagnosis of HLH secondary to solid neoplasm was formulated. The patient started chemotherapy with oxaliplatin, calcium levofolinate, 5-fluorouracil bolus, 5-fluorouracil for 48 h (mFOLFOX6), and methylprednisolone. Six days after the third cycle of mFOLFOX6, the patient was discharged with the stabilization of his piastrinopenia condition. The patient continued chemotherapy with an improvement in his clinical conditions and normalization of hematological values. After 12 cycles of mFOLFOX, it was decided to start maintenance chemotherapy with capecitabine but, unfortunately, after only one cycle, HLH reappeared. The oncologist has to keep in mind the existence of HLH when there is an unusual clinical presentation of cancer, such as cytopenia affecting ≥2 lineages and alterations of ferritin and triglycerides other than fibrinogen and coagulation. Increased attention and additional research as well as a close collaboration with hematologists are needed to benefit patients with solid tumors complicated by HLH., Competing Interests: GM worked as a consultant for Astellas and Servier; received honoraria and participated in the speaker bureau of Astellas, Menarini/Stemline, Pfizer, Servier, and Syros; and received research support from Abbvie, Astrazeneca, and Pfizer. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Monti, Marconi, Ambrosini-Spaltro, Gallio, Ghini, Esposito, Antonini, Montanari and Frassineti.)
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- 2023
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34. "Surgery First" vs "Traditional Sequence" Surgery: A Qualitative Study of Health Experiences in 46 Bimaxillary Orthognathic Patients.
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Beccuti ML, Cozzani M, Antonini S, Doldo T, and Raffaini M
- Abstract
Introduction: Orthognathic patients are advocating an active role in selecting their appropriate ortho-surgical treatment, between the surgery first (SF) and the traditional sequence (TS) approaches. The aim of this study was to evaluate, through qualitative analysis, the subjective perceptions of the outcomes of each protocol., Methods: In-depth interviews were conducted with 46 (10 male and 36 female) orthognathic patients (23 SF and 23 TS) treated with bimaxillary orthognathic surgery by the same surgeon, between 2013 and 2015. Average treatment duration was 6.5 months for SF and 12 months for TS. Inclusion criteria were: the presence of Class III or Class II asymmetries and open bite. Patients were excluded if they refused interviews or stopped attending post-treatment follow-up. Investigated health experiences included overall satisfaction with appearance, self-confidence after surgery, perceived treatment time, functional recovery, and diet restrictions., Results: All SF and TS patients showed overall satisfaction with their appearance (though TS showed more enthusiastic tones) and approved their degree of functional recovery after surgery. Class III SF patients had earlier improvements in self-confidence after surgery. Orthodontics was considered enduring by both SF and TS patients., Conclusions: SF patients expressed a higher degree of satisfaction with the reduction in overall treatment time and with the early psychological benefit deriving therefrom. Both SF and TS patients completely approved of the aesthetic outcomes and the functional recovery from which they benefitted due to the entire procedure., Competing Interests: Conflict of interestThe authors have no conflicts of interest to declare and report no proprietary, commercial, or financial interest in the companies or products described in this article., (© The Association of Oral and Maxillofacial Surgeons of India 2021.)
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- 2022
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35. Osilodrostat in Cushing's disease: the management of its efficacy and the pitfalls of post-surgical results.
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Antonini S, Brunetti A, Zampetti B, Boeris D, Saladino A, and Cesare Cozzi R
- Abstract
Summary: Osilodrostat is a novel, orally administered cortisol synthesis inhibitor, approved in 2020 by the European Medicines Agency (EMA) for the treatment of Cushing's syndrome in adults. A significant amount of the studies currently available in the literature focus on treatment in patients with Cushing's disease. However, data collected from patients treated with osilodrostat in real-life settings still represents a small entity. For this reason, in this article, we will discuss two real-life cases of patients with Cushing's disease treated with this drug. The first report is about a 35-year-old woman with an adrenocorticotrophic hormone (ACTH)-secreting adenoma. After non-curative trans-nasal-sphenoidal (TNS) surgery, due to a small remnant of the adenoma, medical therapy with osilodrostat achieved fast and effective biochemical and clinical response. During treatment, progressive increase of ACTH levels and an enlargement of the pituitary remnant were documented, with planned radiosurgical treatment. The second case reports a 32-year-old man diagnosed with Cushing's disease in 2020, who, after surgery refusal, started osilodrostat at progressively up-titrated doses, according to 24 h urinary free cortisol levels, up to 5 mg twice a day. With osilodrostat, the patient reached biochemical and clinical control of disease until TNS surgery in October 2021, with complete remission. The first post-surgical biochemical assessment was equivocal in spite of a transient clinical hypoadrenalism, reverted after 2 months with the restoration of physiological hypothalamic-pituitary-adrenal axis (HPA) function., Learning Points: Osilodrostat is a potent oral drug viable for Cushing's disease as medical therapy when surgery is not feasible or remission cannot be reached. Osilodrostat proves to be a safe drug and its main adverse effect is hypoadrenalism, due to the adrenolytic action of the compound. Osilodrostat needs a very tailored approach in its clinical use because there is no correlation between the level of hypercortisolism pre-treatment and the dose required to reach disease control.
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- 2022
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36. SCROTAL THERMOGRAPHY IN THE EVALUATION OF SURGICAL OUTCOME AFTER VARICOCELE REPAIR: A CASE REPORT.
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Karlović K, Kuliš T, Lukić I, Kolarić D, Milas I, Miškić B, Antonini S, and Kaštelan Ž
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- Male, Humans, Thermography methods, Physical Examination, Treatment Outcome, Scrotum, Varicocele diagnosis, Varicocele surgery
- Abstract
Scrotal thermography is a diagnostic method for varicocele. In short, there are five diagnostic thermographic criteria for varicocele, i.e., pattern of scrotal thermographic image indicative of varicocele, temperature at pampiniform plexus ≥34 C°, temperature difference between left and right pampiniform plexus ≥0.5 C°, enhancement of image during Valsalva maneuver, and temperature at pampiniform plexus ≥ temperature at ipsilateral thigh. Three or more positive signs are indicative of varicocele. The aim of this report is to present the use of digital thermography as a diagnostic method to evaluate the outcome of varicocele repair. We present a case of a student diagnosed with varicocele grade III, and assessed preoperatively and followed up postoperatively by scrotal thermography. According to thermographic indicators, our patient was positive for varicocele diagnosis before surgical treatment. Three months after varicocele repair, the patient did not show positive thermographic indicators of varicocele while physical examination and color Doppler ultrasound were equivocal. This case report suggests that infrared digital thermography of scrotum could be very valuable for monitoring patients in the period after surgery for varicocele, however, it should be confirmed in a larger number of patients.
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- 2022
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37. Clinical Management of Acromegaly: Therapeutic Frontiers and New Perspectives for Somatostatin Receptor Ligands (SRLs).
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Brunetti A, Antonini S, Saladino A, Lavezzi E, Zampetti B, and Cozzi R
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- Humans, Ligands, Receptors, Somatostatin therapeutic use, Acromegaly drug therapy
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Somatostatin receptor ligands (SRLs) represent a true milestone in the medical therapy for acromegaly. The first-generation SRLs (FG-SRLs), octreotide and lanreotide, have demonstrated good efficacy in disease control and tumor shrinkage, and are still considered first-line medical therapies. The development of long-acting release (LAR) formulations has certainly improved the therapeutic tolerability of these drugs, although many patients still experience therapy-related burden. As such, new formulations have recently been developed to improve adherence and therapeutic efficacy and more solutions are on the way. In the case of FG-SRL-resistant disease, pasireotide, the only second generation SRL currently available, demonstrated superiority in disease control and tumor shrinkage compared to FG-SRLs. However, its use in clinical practice is still limited due to concern for impairment in glucose homeostasis. In this review, we discuss the news about the present and future role of SRLs in acromegaly, exploring the therapeutical frontiers of this drug class. Moreover, we provide practical guidance on the use of pasireotide, based on the data in the literature and our clinical experience.
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- 2022
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38. Recurrent pneumothorax in a patient with liposarcoma as either a complication of lung micrometastasis or a potential adverse event from antibiotic therapy: A case report.
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Monti M, Sullo FG, Iamurri AP, Gianni C, Silimbani P, Bartolini G, Valgiusti M, Esposito L, Montanari D, Antonini S, and Frassineti GL
- Abstract
Spontaneous pneumothorax (PNX) is an infrequent manifestation of primary lung cancer, soft tissue sarcoma and metastasis. There are no easily accessible data in the literature regarding the correlation between PNX and antibiotics, whereas cases of PNX following chemotherapy have been observed. Only 1-10% of treatment-related adverse events are estimated to be reported to the Food and Drug Administration. The present study described a case of PNX of the left lung in a 70-year-old treatment-naive patient with retroperitoneal liposarcoma. The PNX developed after 8 days of treatment with levofloxacin and after 6 days of piperacillin/tazobactam treatment for a suspicious inflammatory area in the right lung detected by an FDG-PET scan before the patient started chemotherapy. A chest CT scan confirmed the presence of metastasis in the right lung, but neither FDG-PET/CT nor CT showed metastatic disease in the left lung. A total of 14 days after the end of the third cycle of doxorubicin (2 months after the initial diagnosis of PNX), the patient manifested a massive PNX of the right lung. In conclusion, these findings indicated that spontaneous PNX could be linked to the use of some antibiotics., Competing Interests: The authors declare that they have no competing interests., (Copyright: © Monti et al.)
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- 2022
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39. Prevention of recurrent respiratory infections : Inter-society Consensus.
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Chiappini E, Santamaria F, Marseglia GL, Marchisio P, Galli L, Cutrera R, de Martino M, Antonini S, Becherucci P, Biasci P, Bortone B, Bottero S, Caldarelli V, Cardinale F, Gattinara GC, Ciarcià M, Ciofi D, D'Elios S, Di Mauro G, Doria M, Indinnimeo L, Lo Vecchio A, Macrì F, Mattina R, Miniello VL, Del Giudice MM, Morbin G, Motisi MA, Novelli A, Palamara AT, Panatta ML, Pasinato A, Peroni D, Perruccio K, Piacentini G, Pifferi M, Pignataro L, Sitzia E, Tersigni C, Torretta S, Trambusti I, Trippella G, Valentini D, Valentini S, Varricchio A, Verga MC, Vicini C, Zecca M, and Villani A
- Subjects
- Adenoidectomy, Adjuvants, Immunologic therapeutic use, Administration, Intranasal, Algorithms, Antibiotic Prophylaxis, Antioxidants administration & dosage, Child, Complementary Therapies, Humans, Hyaluronic Acid administration & dosage, Influenza Vaccines, Pneumococcal Vaccines, Prebiotics, Probiotics therapeutic use, Pyrrolidonecarboxylic Acid analogs & derivatives, Pyrrolidonecarboxylic Acid therapeutic use, Recurrence, Resveratrol administration & dosage, Thiazolidines therapeutic use, Tonsillectomy, Vitamins therapeutic use, Respiratory Tract Infections prevention & control
- Abstract
Recurrent respiratory infections (RRIs) are a common clinical condition in children, in fact about 25% of children under 1 year and 6% of children during the first 6 years of life have RRIs. In most cases, infections occur with mild clinical manifestations and the frequency of episodes tends to decrease over time with a complete resolution by 12 years of age. However, RRIs significantly reduce child and family quality of life and lead to significant medical and social costs.Despite the importance of this condition, there is currently no agreed definition of the term RRIs in the literature, especially concerning the frequency and type of infectious episodes to be considered. The aim of this consensus document is to propose an updated definition and provide recommendations with the intent of guiding the physician in the complex process of diagnosis, management and prevention of RRIs., (© 2021. The Author(s).)
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- 2021
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40. Visual System Impairment in a Mouse Model of Krabbe Disease: The Twitcher Mouse.
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Tonazzini I, Cerri C, Del Grosso A, Antonini S, Allegra M, Caleo M, and Cecchini M
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- Animals, Brain metabolism, Brain pathology, Disease Models, Animal, Electrophysiological Phenomena, Galactosylceramidase metabolism, Humans, Leukodystrophy, Globoid Cell metabolism, Leukodystrophy, Globoid Cell pathology, Lysosomal Storage Diseases metabolism, Lysosomal Storage Diseases pathology, Mice, Myelin Sheath metabolism, Psychosine genetics, Psychosine metabolism, Visual Cortex pathology, Galactosylceramidase genetics, Leukodystrophy, Globoid Cell genetics, Lysosomal Storage Diseases genetics, Visual Cortex metabolism
- Abstract
Krabbe disease (KD, or globoid cell leukodystrophy; OMIM #245200) is an inherited neurodegenerative condition belonging to the class of the lysosomal storage disorders. It is caused by genetic alterations in the gene encoding for the enzyme galactosylceramidase, which is responsible for cleaving the glycosydic linkage of galatosylsphingosine (psychosine or PSY), a highly cytotoxic molecule. Here, we describe morphological and functional alterations in the visual system of the Twitcher (TWI) mouse, the most used animal model of Krabbe disease. We report in vivo electrophysiological recordings showing defective basic functional properties of the TWI primary visual cortex. In particular, we demonstrate a reduced visual acuity and contrast sensitivity, and a delayed visual response. Specific neuropathological alterations are present in the TWI visual cortex, with reduced myelination, increased astrogliosis and microglia activation, and around the whole brain. Finally, we quantify PSY content in the brain and optic nerves by high-pressure liquid chromatography-mass spectrometry methods. An increasing PSY accumulation with time, the characteristic hallmark of KD, is found in both districts. These results represent the first complete characterization of the TWI visual system. Our data set a baseline for an easy testing of potential therapies for this district, which is also dramatically affected in KD patients.
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- 2020
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41. A New Proposal: a Digital Flow for the Construction of a Haas-Inspired Rapid Maxillary Expander (HIRME).
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Cozzani M, Antonini S, Lupini D, Decesari D, Anelli F, and Doldo T
- Abstract
Maxillary expansion is a common orthodontic treatment used for the correction of posterior crossbite resulting from reduced maxillary width. Transverse maxillomandibular discrepancies are a major cause of several malocclusions and may be corrected in different manners; in particular, the rapid maxillary expansion (RME) performed in the early mixed dentition has now become a routine procedure in orthodontic practice. The aim of this study is to propose a procedure that reduces the patient cooperation as well as the lab work required in preparing a customized Haas-inspired rapid maxillary expander (HIRME) that can be anchored to deciduous teeth and can be utilized in mixed dentition with tubes on the molars and hooks and brackets on the canines. This article thus presents an expander that is completely digitally developed, from the first moment of taking the impression with an optical scanner to the final solidification phase by the use of a 3D printer. This digital flow takes place in a CAD environment and it starts with the creation of the appliance on the optical impression; this design is then exported as an stl extension and is sent to the print service to obtain a solid model of the device through a laser sintering process. This "rough" device goes through a post-processing procedure; finally, a commercial expansion screw is laser-welded. This expander has all the advantages of a cast-metal Haas-type RME that rests on deciduous teeth; moreover, it has the characteristic of being developed with a completely digitized and individualized process, for the mouth of the young patient, as well as being made completely of cobalt-chrome, thus ensuring greater adaptability and stability in the patient's mouth., Competing Interests: The authors declare no conflict of interest
- Published
- 2020
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42. The Transcription Factor Nfix Requires RhoA-ROCK1 Dependent Phagocytosis to Mediate Macrophage Skewing during Skeletal Muscle Regeneration.
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Saclier M, Lapi M, Bonfanti C, Rossi G, Antonini S, and Messina G
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- Animals, Cell Differentiation, Cell Proliferation, Inflammation, Macrophages metabolism, Mice, Mice, Knockout, Muscle, Skeletal metabolism, Myoblasts, Skeletal cytology, NFI Transcription Factors genetics, Macrophages physiology, Muscle, Skeletal physiology, NFI Transcription Factors metabolism, Phagocytosis physiology, Regeneration, rho-Associated Kinases metabolism, rhoA GTP-Binding Protein metabolism
- Abstract
Macrophages (MPs) are immune cells which are crucial for tissue repair. In skeletal muscle regeneration, pro-inflammatory cells first infiltrate to promote myogenic cell proliferation, then they switch into an anti-inflammatory phenotype to sustain myogenic cells differentiation and myofiber formation. This phenotypical switch is induced by dead cell phagocytosis. We previously demonstrated that the transcription factor Nfix, a member of the nuclear factor I (Nfi) family, plays a pivotal role during muscle development, regeneration and in the progression of muscular dystrophies. Here, we show that Nfix is mainly expressed by anti-inflammatory macrophages. Upon acute injury, mice deleted for Nfix in myeloid line displayed a significant defect in the process of muscle regeneration. Indeed, Nfix is involved in the macrophage phenotypical switch and macrophages lacking Nfix failed to adopt an anti-inflammatory phenotype and interact with myogenic cells. Moreover, we demonstrated that phagocytosis induced by the inhibition of the RhoA-ROCK1 pathway leads to Nfix expression and, consequently, to acquisition of the anti-inflammatory phenotype. Our study identified Nfix as a link between RhoA-ROCK1-dependent phagocytosis and the MP phenotypical switch, thus establishing a new role for Nfix in macrophage biology for the resolution of inflammation and tissue repair., Competing Interests: The authors declare no conflict of interest.
- Published
- 2020
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43. Nutritional intervention with cyanidin hinders the progression of muscular dystrophy.
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Saclier M, Bonfanti C, Antonini S, Angelini G, Mura G, Zanaglio F, Taglietti V, Romanello V, Sandri M, Tonelli C, Petroni K, Cassano M, and Messina G
- Subjects
- Animals, Disease Models, Animal, Disease Progression, Female, Male, Mice, Knockout, Mitochondria, Muscle pathology, Muscle, Skeletal pathology, Organelle Biogenesis, Phenotype, Protein Carbonylation, Sarcoglycanopathies genetics, Sarcoglycanopathies metabolism, Sarcoglycanopathies pathology, Sarcoglycans deficiency, Sarcoglycans genetics, Anthocyanins administration & dosage, Dietary Supplements, Inflammation Mediators metabolism, Mitochondria, Muscle metabolism, Muscle, Skeletal metabolism, Oxidative Stress, Sarcoglycanopathies diet therapy
- Abstract
Muscular Dystrophies are severe genetic diseases due to mutations in structural genes, characterized by progressive muscle wasting that compromises patients' mobility and respiratory functions. Literature underlined oxidative stress and inflammation as key drivers of these pathologies. Interestingly among different myofiber classes, type I fibers display a milder dystrophic phenotype showing increased oxidative metabolism. This work shows the benefits of a cyanidin-enriched diet, that promotes muscle fiber-type switch and reduced inflammation in dystrophic alpha-sarcoglyan (Sgca) null mice having, as a net outcome, morphological and functional rescue. Notably, this benefit is achieved also when the diet is administered in dystrophic animals when the signs of the disease are seriously evident. Our work provides compelling evidence that a cyanidin-rich diet strongly delays the progression of muscular dystrophies, paving the way for a combinatorial approach where nutritional-based reduction of muscle inflammation and oxidative stress facilitate the successful perspectives of definitive treatments.
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- 2020
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44. Autologous Cell Therapy Approach for Duchenne Muscular Dystrophy using PiggyBac Transposons and Mesoangioblasts.
- Author
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Iyer PS, Mavoungou LO, Ronzoni F, Zemla J, Schmid-Siegert E, Antonini S, Neff LA, Dorchies OM, Jaconi M, Lekka M, Messina G, and Mermod N
- Subjects
- Animals, Cell Line, Disease Models, Animal, Dystrophin genetics, Fluorescent Antibody Technique, Gene Dosage, Gene Expression, Gene Order, Genes, Reporter, Male, Mice, Mice, Inbred mdx, Mice, SCID, Muscular Dystrophy, Duchenne pathology, Muscular Dystrophy, Duchenne physiopathology, Muscular Dystrophy, Duchenne therapy, Phenotype, Transgenes, Transplantation, Autologous, Cell- and Tissue-Based Therapy methods, DNA Transposable Elements, Gene Transfer Techniques, Genetic Vectors genetics, Muscular Dystrophy, Duchenne genetics, Myoblasts metabolism, Myoblasts transplantation
- Abstract
Duchenne muscular dystrophy (DMD) is a lethal muscle-wasting disease currently without cure. We investigated the use of the PiggyBac transposon for full-length dystrophin expression in murine mesoangioblast (MABs) progenitor cells. DMD murine MABs were transfected with transposable expression vectors for full-length dystrophin and transplanted intramuscularly or intra-arterially into mdx/SCID mice. Intra-arterial delivery indicated that the MABs could migrate to regenerating muscles to mediate dystrophin expression. Intramuscular transplantation yielded dystrophin expression in 11%-44% of myofibers in murine muscles, which remained stable for the assessed period of 5 months. The satellite cells isolated from transplanted muscles comprised a fraction of MAB-derived cells, indicating that the transfected MABs may colonize the satellite stem cell niche. Transposon integration site mapping by whole-genome sequencing indicated that 70% of the integrations were intergenic, while none was observed in an exon. Muscle resistance assessment by atomic force microscopy indicated that 80% of fibers showed elasticity properties restored to those of wild-type muscles. As measured in vivo, transplanted muscles became more resistant to fatigue. This study thus provides a proof-of-principle that PiggyBac transposon vectors may mediate full-length dystrophin expression as well as functional amelioration of the dystrophic muscles within a potential autologous cell-based therapeutic approach of DMD., (Copyright © 2018 The American Society of Gene and Cell Therapy. Published by Elsevier Inc. All rights reserved.)
- Published
- 2018
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45. Silencing Nfix rescues muscular dystrophy by delaying muscle regeneration.
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Rossi G, Bonfanti C, Antonini S, Bastoni M, Monteverde S, Innocenzi A, Saclier M, Taglietti V, and Messina G
- Subjects
- Animals, Female, Gene Silencing, Male, Mice, Muscles pathology, Muscular Dystrophies pathology, Sarcoglycans genetics, Muscles physiology, Muscular Dystrophies genetics, NFI Transcription Factors physiology, Regeneration
- Abstract
Muscular dystrophies are severe disorders due to mutations in structural genes, and are characterized by skeletal muscle wasting, compromised patient mobility, and respiratory functions. Although previous works suggested enhancing regeneration and muscle mass as therapeutic strategies, these led to no long-term benefits in humans. Mice lacking the transcription factor Nfix have delayed regeneration and a shift toward an oxidative fiber type. Here, we show that ablating or silencing the transcription factor Nfix ameliorates pathology in several forms of muscular dystrophy. Silencing Nfix in postnatal dystrophic mice, when the first signs of the disease already occurred, rescues the pathology and, conversely, Nfix overexpression in dystrophic muscles increases regeneration and markedly exacerbates the pathology. We therefore offer a proof of principle for a novel therapeutic approach for muscular dystrophies based on delaying muscle regeneration.
- Published
- 2017
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46. Quantum dots labelling allows detection of the homing of mesenchymal stem cells administered as immunomodulatory therapy in an experimental model of pancreatic islets transplantation.
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Mannucci S, Calderan L, Quaranta P, Antonini S, Mosca F, Longoni B, Marzola P, and Boschi F
- Subjects
- Animals, Male, Models, Animal, Optical Imaging, Rats, Rats, Inbred Lew, Rats, Wistar, Cell Movement physiology, Islets of Langerhans Transplantation methods, Mesenchymal Stem Cell Transplantation methods, Quantum Dots
- Abstract
Cell transplantation is considered a promising therapeutic approach in several pathologies but still needs innovative and non-invasive imaging technologies to be validated. The use of mesenchymal stem cells (MSCs) attracts major interest in clinical transplantation thanks to their regenerative properties, low immunogenicity and ability to regulate immune responses. In several animal models, MSCs are used in co-transplantation with pancreatic islets (PIs) for the treatment of type I diabetes, supporting graft survival and prolonging normal glycaemia levels. In this study we investigated the homing of systemically administered MSCs in a rat model of pancreatic portal vein transplantation. MSCs labelled with quantum dots (Qdots) were systemically injected by tail vein and monitored by optical fluorescence imaging. The fluorescence signal of the liver in animals co-transplanted with MSCs and PIs was significantly higher than in control animals in which MSCs alone were transplanted. By using magnetic labelling of PIs, the homing of PIs into liver was independently confirmed. These results demonstrate that MSCs injected in peripheral blood vessels preferentially accumulate into liver when PIs are transplanted in the same organ. Moreover, we prove that bimodal MRI-fluorescence imaging allows specific monitoring of the fate of two types of cells., (© 2016 Anatomical Society.)
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- 2017
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47. Nanotopography Induced Human Bone Marrow Mesangiogenic Progenitor Cells (MPCs) to Mesenchymal Stromal Cells (MSCs) Transition.
- Author
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Antonini S, Montali M, Jacchetti E, Meucci S, Parchi PD, Barachini S, Panvini FM, Pacini S, Petrini I, and Cecchini M
- Abstract
Mesangiogenic progenitor cells (MPCs) are a very peculiar population of cells present in the human adult bone marrow, only recently discovered and characterized. Owing to their differentiation potential, MPCs can be considered progenitors for mesenchymal stromal cells (MSCs), and for this reason they potentially represent a promising cell population to apply for skeletal tissue regeneration applications. Here, we evaluate the effects of surface nanotopography on MPCs, considering the possibility that this specific physical stimulus alone can trigger MPC differentiation toward the mesenchymal lineage. In particular, we exploit nanogratings to deliver a mechanical, directional stimulus by contact interaction to promote cell morphological polarization and stretching. Following this interaction, we study the MPC-MSC transition by i. analyzing the change in cell morphotype by immunostaining of the key cell-adhesion structures and confocal fluorescence microscopy, and ii. quantifying the expression of cell-phenotype characterizing markers by flow cytometry. We demonstrate that the MPC mesengenic differentiation can be induced by the solely interaction with the NGs, in absence of any other external, chemical stimulus. This aspect is of particular interest in the case of multipotent progenitors as MPCs that, retaining both mesengenic and angiogenic potential, possess a high clinical appeal.
- Published
- 2016
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48. Nfix Regulates Temporal Progression of Muscle Regeneration through Modulation of Myostatin Expression.
- Author
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Rossi G, Antonini S, Bonfanti C, Monteverde S, Vezzali C, Tajbakhsh S, Cossu G, and Messina G
- Subjects
- Animals, Cell Differentiation, Cell Line, Cells, Cultured, Gene Silencing, Mice, Transgenic, Myoblasts physiology, Myostatin metabolism, Muscle Fibers, Skeletal physiology, Myostatin genetics, NFI Transcription Factors physiology, Regeneration
- Abstract
Nfix belongs to a family of four highly conserved proteins that act as transcriptional activators and/or repressors of cellular and viral genes. We previously showed a pivotal role for Nfix in regulating the transcriptional switch from embryonic to fetal myogenesis. Here, we show that Nfix directly represses the Myostatin promoter, thus controlling the proper timing of satellite cell differentiation and muscle regeneration. Nfix-null mice display delayed regeneration after injury, and this deficit is reversed upon in vivo Myostatin silencing. Conditional deletion of Nfix in satellite cells results in a similar delay in regeneration, confirming the functional requirement for Nfix in satellite cells. Moreover, mice lacking Nfix show reduced myofiber cross sectional area and a predominant slow twitching phenotype. These data define a role for Nfix in postnatal skeletal muscle and unveil a mechanism for Myostatin regulation, thus providing insights into the modulation of its complex signaling pathway., (Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2016
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49. PW1/Peg3 expression regulates key properties that determine mesoangioblast stem cell competence.
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Bonfanti C, Rossi G, Tedesco FS, Giannotta M, Benedetti S, Tonlorenzi R, Antonini S, Marazzi G, Dejana E, Sassoon D, Cossu G, and Messina G
- Subjects
- Animals, Blotting, Western, Cells, Cultured, Chromatin Immunoprecipitation, DNA Primers genetics, Dogs, Gene Silencing, Genetic Vectors genetics, Humans, Kruppel-Like Transcription Factors genetics, Luciferases, Mice, Microscopy, Fluorescence, Muscle Development physiology, Muscular Dystrophies therapy, MyoD Protein metabolism, Retroviridae, Reverse Transcriptase Polymerase Chain Reaction, Biomarkers metabolism, Blood Vessels cytology, Kruppel-Like Transcription Factors metabolism, Stem Cells physiology
- Abstract
Mesoangioblasts are vessel-associated progenitor cells that show therapeutic promise for the treatment of muscular dystrophy. Mesoangioblasts have the ability to undergo skeletal muscle differentiation and cross the blood vessel wall regardless of the developmental stage at which they are isolated. Here we show that PW1/Peg3 is expressed at high levels in mesoangioblasts obtained from mouse, dog and human tissues and its level of expression correlates with their myogenic competence. Silencing PW1/Peg3 markedly inhibits myogenic potential of mesoangioblasts in vitro through MyoD degradation. Moreover, lack of PW1/Peg3 abrogates mesoangioblast ability to cross the vessel wall and to engraft into damaged myofibres through the modulation of the junctional adhesion molecule-A. We conclude that PW1/Peg3 function is essential for conferring proper mesoangioblast competence and that the determination of PW1/Peg3 levels in human mesoangioblasts may serve as a biomarker to identify the best donor populations for therapeutic application in muscular dystrophies.
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- 2015
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50. Pathogenic mutations in GLI2 cause a specific phenotype that is distinct from holoprosencephaly.
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Bear KA, Solomon BD, Antonini S, Arnhold IJ, França MM, Gerkes EH, Grange DK, Hadley DW, Jääskeläinen J, Paulo SS, Rump P, Stratakis CA, Thompson EM, Willis M, Winder TL, Jorge AA, Roessler E, and Muenke M
- Subjects
- Abnormalities, Multiple pathology, Face pathology, Fingers pathology, Holoprosencephaly, Humans, Infant, Phenotype, Toes pathology, Zinc Finger Protein Gli2, Abnormalities, Multiple genetics, Kruppel-Like Transcription Factors genetics, Mutation genetics, Nuclear Proteins genetics
- Abstract
Background: Mutations in GLI2 have been associated with holoprosencephaly (HPE), a neuroanatomic anomaly resulting from incomplete cleavage of the developing forebrain, and an HPE-like phenotype involving pituitary anomalies and polydactyly., Objective: To characterise the genotypic and phenotypic findings in individuals with GLI2 variants and clarify clinical findings in individuals with loss-of-function mutations., Methods: Through the National Institutes of Health and collaborating centres, ∼400 individuals with HPE spectrum disorders, endocrine disorders or craniofacial anomalies were screened for GLI2 mutations. Results were combined with all published cases. We compared the clinical and molecular features of individuals with truncating mutations to individuals with variants of unknown significance (defined as not resulting in protein truncation, reported in normal controls and/or deemed unlikely to be pathogenic by functional prediction software)., Results: 112 individuals with variants in GLI2 were identified, with 43 having truncating mutations. Individuals with truncating mutations were more likely to have both pituitary anomalies and polydactyly versus those with variants of unknown significance (p<0.0001 by Fisher's exact test); only 1 of 43 had frank HPE. These individuals were more likely to have recognised penetrance (polydactyly or pituitary anomalies or both) than those without truncating mutations (p=0.0036 by Fisher's exact test). A common facial phenotype was seen in individuals (with midface hypoplasia, cleft lip/palate and hypotelorism) with truncating mutations., Conclusions: Individuals with truncating mutations in GLI2 typically present with pituitary anomalies, polydactyly and subtle facial features rather than HPE. This will be helpful in screening populations for GLI2 mutations and for counselling affected patients., Trial Registration: 98-HG-0249/04-HG-0093., (Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.)
- Published
- 2014
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