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The aim of this in vitro study was to evaluate the shear bond strength (SBS) and adhesive remnant index (ARI) scores of two different adhesive applications of aesthetic brackets produced from permanent crown material using 3D-printing technology.

Objectives: To determine if an association exists between the prevalence of white spot lesions (WSLs) and orthodontic treatment using clear aligners.

Wheat is an important staple crop in arid and semi-arid areas of the Tigray region of Ethiopia. However, the yield and quality of the crop is markedly constrained by low soil fertility, inappropriate rate and timing of nitrogen application. Therefore, an experiment was conducted to elucidate the effect of different rates and timing of nitrogen fertilizer application on the yield, quality, and nitrogen use efficiency of bread wheat varieties during 2013 and 2014 main cropping seasons. The treatments consisted of four rates of nitrogen (23, 46, 69, and 92 kg N ha-1), three timing of N application (½ at sowing + ½ at tillering, ¼ at sowing + ½ at tillering + ¼ at anthesis, and 1/3rd at sowing + 1/3 rd at tillering + 1/3rd at anthesis) and two bread wheat varieties (Picaflor and Mekelle I) and 0 kg N ha-1 (control). The experiment was laid out as a randomized complete block design in a factorial arrangement with three replications. During both cropping years, increasing the rate of nitrogen application up to 69 kg N ha-1 significantly (P< 0.01) increased the total aboveground biomass and grain yields of the crop and decreased with further increase of applied N fertilizer. The highest aboveground biomass (6871 kg ha-1) and grain yields (2775 kg ha-1) of the crop were obtained in response to the application of 69 kg N ha-1, in 2013. The equivalent value for total aboveground biomass and grain yields during 2014 cropping season were (8815 kg ha-1and 3695 kg ha-1), respectively . The highest apparent recovery efficiency of 75% was obtained in response to the rate of 69 kg N ha-1applied in three splits of 1/4 at planting, ½ at tillering, and 1/4 at anthesis. The equivalent values for the highest apparent recovery was 81% in the 2014 cropping season. The highest agronomic efficiency of 25 kg kg-1 and utilization efficiency of 55 kg kg-1 were recorded at the rate of 46 kg N ha-1 in three splits of 1/4 at planting, ½ at tillering and ¼ at anthesis. The equivalent values in 2014 were 34 kg kg-1 and 78 kg kg-1, respectively. Grain protein concentration increased from 10.57% to 11.82% in 2013 and from 8.97% to 9.84% in 2014 cropping season as the rate increased from 23 kg N ha-1 to 69 kg N ha-1. The acceptable marginal rate of return (1618%) and highest net benefit (34141ETB ha-1) was obtained from 69 kg N ha-1 for the variety Picaflor. In conclusion, applying 69 kg N ha-1 in three splits of ¼ at planting, 1/2 at tillering and ¼ at anthesis resulted in optimum grain yield, protein concentration and economic return of the crop.

Drought is a significant factor that causes yield loss in essential cereal crops such as sorghum [Sorghum bicolor (L.) Moench], necessitating the development of drought-tolerant varieties adaptable to various water conditions. This study aimed to pinpoint drought-tolerant sorghum lines and genomic regions for tolerance by utilizing 216 sorghum accessions in stressed and non-stressed environments at two locations. Genetic diversity was evident among accessions in terms of grain yield under different watering regimes. Drought stress indices such as the stress tolerance index, mean productivity, geometric mean productivity, harmonic mean productivity, yield stability index, and yield index were identified as effective measures for selecting drought-tolerant sorghum. Cluster analysis classified genotypes into four groups based on their association with grain yield, highlighting Acc. #28546 and Acc. #216739 as highly drought tolerant across environments. This study identified 32 and 22 quantitative trait nucleotides (QTNs) for drought indices and grain yield under stress and non-stress conditions, respectively, at two locations, with five common QTNs linked to multiple drought indices. Colocation analysis revealed that these QTNs were associated with known stay-green-related quantitative trait loci (QTLs), and 47 putative genes near these QTNs potentially influenced drought tolerance traits. It is suggested that accession selection considers multiple indices for robust evaluation. Understanding the identified genes and their functions provides insights into the genetic mechanisms governing plant responses to drought stress, offering prospects for developing improved drought-resistant sorghum varieties through further genetic research., (© 2024 The Author(s). The Plant Genome published by Wiley Periodicals LLC on behalf of Crop Science Society of America.)

Objectives: The aim of this study was to evaluate the effect of in-vivo produced Nisin which is an antimicrobial peptide (AMP) added to adhesive resin on shear bond strength (SBS) and the adhesive remnant index (ARI) of orthodontic brackets., Methods: Bacterial AMP was produced by fermentation and the ideal AMP/Bond concentration and antimicrobial efficacy of the mixture were tested. To evaluate the SBS and ARI scores of AMP-added adhesive resins, 80 maxillary premolar teeth extracted for orthodontic purposes were used and randomly assigned into 2 groups (n = 40). Group 1: Control Group (teeth bonded with standard adhesive resin); Group 2: Experimental Group (teeth bonded with AMP-added adhesive resin). Statistical analysis was performed using the SPSS package program and applying the Mann-Whitney U and Fisher's exact tests. P < 0.05 was considered as statistically significant., Results: Nisin synthesized in-vivo from Lactococcus lactis (L. lactis) (ATCC 7962) bacteria was provided to form a homogenous solution at an ideal concentration To find the minimum AMP/Bond mixture ratio that showed maximum antimicrobial activity, AMP and Bond mixtures were tested at various concentration levels between 1/160 and 1/2 (AMP/Bond). As a result, the optimum ratio was determined as 1/40. The antimicrobial efficacy of Nisin-added adhesive resin was tested against Streptococcus mutans (S. mutans) (ATCC 35,688) and Lactobacillus strains (cariogenic microorganisms). AMP formed a 2.7 cm diameter zone alone, while 1/40 AMP-bond mixture formed a 1.2 cm diameter zone. SBS values of the teeth bonded with Nisin added adhesive (17.49 ± 5.31) were significantly higher than the control group (14.54 ± 4.96) (P = 0.004). According to the four point scale, Nisin added adhesive provided a higher ARI score in favour of the adhesive and tooth compared to the control group (ARI = 3, n = 20)., Conclusions: Nisin produced from L. lactis (ATCC 7962) had greater antimicrobial effects after mixing with adhesive bond against cariogenic microorganisms S. mutans (ATCC 35,688) and Lactobacillus strains. Nisin added adhesive increased shear bond strength (SBS) of orthodontic brackets and ARI scores in favor of adhesive & teeth., Clinical Relevance: Clinicians should take into account that using Nisin-added adhesive resin in orthodontic treatments can provide prophylaxis against tooth decay, especially in patients with poor oral hygiene., (© 2024. The Author(s).)

Ethiopian subsistence farmers traditionally store their grain harvests, leaving them open to storage pests and fungi that can cause contamination of major staple crops. Applying the most effective strategy requires a precise understanding of the insect species, infestation rates, storage losses, and storage conditions in the various types of farmers' grain stores. This study did a complete literature analysis on post-harvest pest and management measures with a focus on Ethiopia. The most frequent insect pests of stored cereals in this study were weevils ( Sitophilus spp.), the lesser grain borer ( Rhyzopertha dominica ), rust-red flour beetle ( Tribolium sp.), sawtoothed grain beetle ( Oryzaephilus sp.), grain beetle ( Cryptolestes spp.), Indian meal moth ( Plodia interpunctella ), and Angoumois grain moth ( Sitotroga cerealella ). Flour beetles ( Tribolium spp.), sawtoothed beetles ( Oryzaephilus sp.), flat grain beetles ( Cryptolestes pusillus ), and some moths have been identified as common stored product pests of stored oil seed, while bruchid beetles ( Callosobruchus chinensis ) and the moths were reported for pulses. Additionally, the storage pests in Ethiopia under varied conditions caused storage losses of 9-64.5%, 13-95%, 36.9-51.9%, and 2-94.7% in maize, sorghum, chickpeas, and sesame, respectively. To reduce the losses incurred, preventative measures can be taken before infestations or as soon as infestations are discovered. A variety of pest population monitoring systems for harvested products and retailers have been developed and recommended. In this context, reducing post-harvest grain losses is an urgent concern for improving food accessibility and availability for many smallholder farmers in Ethiopia and ensuring the nation's long-term food security.

Objectives: The present study aimed to determine the levels of happiness among dentists and to investigate their associations with emotional well-being and satisfaction with life., Methods: Dentists in Turkey were surveyed with 25 questions on age, gender, place of work, and years of experience, and a five-item life satisfaction scale., Results: A total of 486 dentists completed the survey. Dentists' workplaces and professional titles were associated with their life satisfaction scores. Most dentists (n = 373; 76.25%) reported feeling pressured in their work environment, and the institution where they worked was significantly associated with the degree of pressure ( p  < 0.001). Institution ( p  < 0.001), job title ( p  < 0.001), and work experience ( p  < 0.019) were significant factors in whether they would recommend their profession to others. Dentists' institutions and titles were significantly associated with life satisfaction ( p  < 0.001)., Conclusions: Our findings suggest that dentists in Turkey have a low life satisfaction with respect to that of other professionals and dentists from other countries. In addition, the factors associated with life satisfaction vary., (© 2022 [The Author/The Authors].)

Background: Mother to child transmission is responsible for 90% of child infection with human immune deficiency virus (HIV). Dual contraceptive use is one of the best actions to prevent mother's human immune deficiency virus transmission to her child and partner. This study aimed at assessing the prevalence and factors associated with dual contraceptive use among sexually active women on antiretroviral therapy in Gondar City, northwest, Ethiopia., Methods: An institution based cross sectional study was conducted in Gondar City public health facilities from December 1 to 31, 2018. Systematic random sampling technique was utilized to include 563 study participants. Data were collected by interview using a structured questionnaire. Descriptive analysis was made to compute mean, median and proportion. Finally, multivariable logistic regression model was fitted to identify the factors associated with dual contraceptive method utilization. Analysis was performed by using Statistical Package for Social Sciences (SPSS) software version 20., Results: The overall prevalence of dual contraceptive method utilization among sexually active women on antiretroviral therapy was 28.8% (95% CI: 24.9, 32.7). Women aged 35-49 years (Adjusted odds ratio (AOR): 6.99; 95% CI: 3.11, 15.71)), who lived in urban areas (AOR: 4.81; 95% CI: 2.04, 11.31), attended secondary and above education (AOR: 4.43; 95% CI: 1.92, 10.22), and disclosed HIV status to sexual partners (AOR: 9.84; 95% CI: 3.48, 27.81) were more likely to use dual contraceptive method., Conclusion: In this study, the proportion of women who utilized dual contraceptive method was low. Age, place of residence, educational status and disclosure of HIV status were factors associated with dual contraceptive use. Therefore, providing education about the advantages of disclosing HIV status to sexual partners and strengthening of counseling about the advantages of dual contraceptive use will be helpful in enhancing the use of dual contraceptive method among sexually active women on antiretroviral therapy.

Background: Hematological abnormalities are common in pulmonary tuberculosis (PTB) patients, which is one of the major public health problems worldwide. However, there is paucity of information about the hematological profile of PTB patients with and without HIV in the study area. Therefore, this study aimed to assess hematological abnormalities of pulmonary tuberculosis patients with and without HIV at the University of Gondar Hospital, Northwest Ethiopia., Methods: A comparative cross-sectional study was conducted at the University of Gondar Hospital. Sociodemographic data was collected using a pretested, structured questionnaire. Five milliliters of venous blood sample was collected and divided into a 3 ml EDTA tube for complete blood count with the Cell Dyn 1800 hematological analyzer and a 2 ml citrated tube for erythrocyte sedimentation rate determination. Data were entered into Epi Info version 3.5.3 and then transferred to SPSS 20 for analysis. The independent samples t -test was used to compare the mean values of hematological parameters between PTB patients and PTB-HIV coinfected patients., Result: A total of 100 study subjects (50 PTB and 50 PTB-HIV coinfected) were included with a mean age of 31.3 ± 10.3 years for PTB patients and 32.1 ± 9.2 years for PTB-HIV coinfected patients. In this study, there were significantly lower mean values of Hgb ( P = 0.049), platelet count ( P < 0.001), and neutrophils counts ( P = 0.007) among PTB-HIV coinfected patients when compared with PTB patients. Of the PTB infected patients 46% were anemic, 6% leukopenic, 22% neutropenic, 8% lymphopenic, and 8% thrombocytopenic. On the other hand, of the PTB-HIV coinfected patients 60% were anemic, 14% leukopenic, 66% neutropenic, 12% lymphopenic, and 20% thrombocytopenic. ESR value was increased in all patients., Conclusion: This study demonstrated high prevalence of neutropenia, anemia, and thrombocytopenia among PTB-HIV coinfected patients. HIV coinfection worsens hematological abnormalities of PTB patients. Assessment of hematological parameters can be used as an indicator in the diagnosis and follow-up of PTB patients coinfected with HIV. We recommended assessment of PTB patients with or without HIV for various hematological disorders such as neutropenia, anemia, and thrombocytopenia.

Lactic acid bacteria (LAB) and yeasts were enumerated and identified from naturally fermented buttermilk. Isolates were first subjected to chemical tests and then to molecular characterization. Molecular identification involved pure sequencing of 16s rRNA (LAB) and 18s rRNA (yeast) genes. Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (DGGE) was used for detection of microbiota composition. Eighty-five LAB and 26 yeast isolates obtained from 16 small-scale dairy farms were isolated and identified. The microbial composition was dominated by strains of Lactococcus lactis ssp. lactis, Lactobacillus plantarum and Saccharomyces cerevisiae. Molecular techniques enabled not only genetic confirmation but also detection of some uncultivated strains. The presence of diverse strains of LAB and yeasts in NFB indicated a potential for development of different starter cultures to make new dairy products.

Farmers' innovation and selection of barley varieties were studied in the Tigray Region in northern Ethiopia. Two districts each in the central and southern zones and three districts in the eastern zone of Tigray were randomly selected for this study, which sought to understand the current status of local barley varieties and to measure their relative preference by farmers. Household surveys were conducted covering 240 households to elicit farmers' views on the values, constraints, and opportunities of growing local varieties of barley. This was supported by focus-group and informal discussions with elders, key informants, and women's groups. Case studies were made of local farmers whom the community recognized as barley breeders. Twenty-four barley varieties and their major descriptors were recorded. Seed and varietal-selection criteria depended on the environmental and varietal characteristics. Investigation of intrahousehold decision making indicated that, while men tended to decide on the type of variety to grow, seed storage and processing were exclusively the responsibility of women. Farmers undertook preharvest and postharvest selection, giving emphasis mainly to earliness and spike characteristics. The distinct varietal-selection and seed-renewal procedures revealed their potential for use in further plant breeding. The case-study analysis of farmer-developed varieties provided knowledge that, if combined with scientists' knowledge, could lead to identification and development of valuable cultivars with a wide potential for use in semiarid areas of Tigray and other parts of Ethiopia.