1. Neuroprotective effects of macranthoin G from Eucommia ulmoides against hydrogen peroxide-induced apoptosis in PC12 cells via inhibiting NF-κB activation.
- Author
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Hu W, Wang G, Li P, Wang Y, Si CL, He J, Long W, Bai Y, Feng Z, and Wang X
- Subjects
- Animals, Caspase 3 metabolism, Catalase metabolism, Chlorogenic Acid pharmacology, Glutathione metabolism, Glutathione Peroxidase metabolism, Hydrogen Peroxide pharmacology, Lipid Peroxidation drug effects, Malondialdehyde metabolism, Membrane Potential, Mitochondrial drug effects, Mitogen-Activated Protein Kinase 3 metabolism, NF-KappaB Inhibitor alpha metabolism, Oxidative Stress drug effects, PC12 Cells, Phosphorylation, Rats, Reactive Oxygen Species metabolism, Signal Transduction, Superoxide Dismutase metabolism, Transcription Factor RelA metabolism, p38 Mitogen-Activated Protein Kinases metabolism, Antioxidants pharmacology, Apoptosis drug effects, Chlorogenic Acid analogs & derivatives, Eucommiaceae chemistry, Neuroprotective Agents pharmacology, Transcription Factor RelA antagonists & inhibitors
- Abstract
Oxidative stress-mediated cellular injury has been considered as a major cause of neurodegenerative diseases including Alzheimer and Parkinson diseases. The scavenging of reactive oxygen species (ROS) mediated by antioxidants may be a potential strategy for retarding the disease's progression. Macranthoin G (MCG), isolated from Eucommia ulmoides, is a derivative from chlorogenic acid methyl ester and caffeic acid. This study is aimed to investigate the protective role of MCG against the cytotoxicity induced by hydrogen peroxide (H2O2) and to elucidate potential protective mechanisms in rat pheochromocytoma (PC12) cells. The results showed that the treatment of PC12 cells with MCG prior to H2O2 exposure effectively increased the cell viability, and stabilized the mitochondria membrane potential (MMP); furthermore, it enhanced the antioxidant enzyme activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) and the levels of intracellular glutathione (GSH); it also decreased the malondialdehyde (MDA) content, intracellular ROS, caspase-3 activation, as well as cell apoptosis. In addition, the MCG treatment minimized the cell injury by H2O2 via down-regulation of the NF-κB pathway as well as activation of phosphorylation of IκBα, p38, and the extracellular signal-regulated kinase (ERK). These results showed that that MCG is promising as a potential therapeutic agent for neurodegenerative diseases induced by oxidative damage and should be encouraged for further research., (Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2014
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