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Neuroprotective effects of macranthoin G from Eucommia ulmoides against hydrogen peroxide-induced apoptosis in PC12 cells via inhibiting NF-κB activation.
- Source :
-
Chemico-biological interactions [Chem Biol Interact] 2014 Dec 05; Vol. 224, pp. 108-16. Date of Electronic Publication: 2014 Oct 19. - Publication Year :
- 2014
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Abstract
- Oxidative stress-mediated cellular injury has been considered as a major cause of neurodegenerative diseases including Alzheimer and Parkinson diseases. The scavenging of reactive oxygen species (ROS) mediated by antioxidants may be a potential strategy for retarding the disease's progression. Macranthoin G (MCG), isolated from Eucommia ulmoides, is a derivative from chlorogenic acid methyl ester and caffeic acid. This study is aimed to investigate the protective role of MCG against the cytotoxicity induced by hydrogen peroxide (H2O2) and to elucidate potential protective mechanisms in rat pheochromocytoma (PC12) cells. The results showed that the treatment of PC12 cells with MCG prior to H2O2 exposure effectively increased the cell viability, and stabilized the mitochondria membrane potential (MMP); furthermore, it enhanced the antioxidant enzyme activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) and the levels of intracellular glutathione (GSH); it also decreased the malondialdehyde (MDA) content, intracellular ROS, caspase-3 activation, as well as cell apoptosis. In addition, the MCG treatment minimized the cell injury by H2O2 via down-regulation of the NF-κB pathway as well as activation of phosphorylation of IκBα, p38, and the extracellular signal-regulated kinase (ERK). These results showed that that MCG is promising as a potential therapeutic agent for neurodegenerative diseases induced by oxidative damage and should be encouraged for further research.<br /> (Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.)
- Subjects :
- Animals
Caspase 3 metabolism
Catalase metabolism
Chlorogenic Acid pharmacology
Glutathione metabolism
Glutathione Peroxidase metabolism
Hydrogen Peroxide pharmacology
Lipid Peroxidation drug effects
Malondialdehyde metabolism
Membrane Potential, Mitochondrial drug effects
Mitogen-Activated Protein Kinase 3 metabolism
NF-KappaB Inhibitor alpha metabolism
Oxidative Stress drug effects
PC12 Cells
Phosphorylation
Rats
Reactive Oxygen Species metabolism
Signal Transduction
Superoxide Dismutase metabolism
Transcription Factor RelA metabolism
p38 Mitogen-Activated Protein Kinases metabolism
Antioxidants pharmacology
Apoptosis drug effects
Chlorogenic Acid analogs & derivatives
Eucommiaceae chemistry
Neuroprotective Agents pharmacology
Transcription Factor RelA antagonists & inhibitors
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7786
- Volume :
- 224
- Database :
- MEDLINE
- Journal :
- Chemico-biological interactions
- Publication Type :
- Academic Journal
- Accession number :
- 25451577
- Full Text :
- https://doi.org/10.1016/j.cbi.2014.10.011